Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005072

Physical Location & Seq

Genomic Coordinates Reverse (-)

28711331 .. 28715380

Gene Structure 4050 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1200600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGGCTGCTGCGAGGGTTGAGCGTGGCGATCTATGGAAGAGCAAGGCCCTGTCGGTGCAGCTCCGGCTCAGGCAGCGGTTCAGGGTGGAGGTGGATCGCCGCCTCCGGCACCACCCCATTTTTTCCAACGACGGTTACTTCTCCTCCACATTGCAGCGTTGGCTCGAGCGATTTCGCGACTTCCGCCGTGATTCCCTCCCTTCCTCCACCGCCTTCTACCGCAAACGAGTGGGTAAGGATTTCAATGCTGAAGAAGAATCAATCCTTCTACGCATGCTTCAAGCTGTGGCTGTTCCGGTCATTGGCAATGTTTGTCATGTGTTTATGAATGGATTGAATCAGGTTCAGGTGTATGGTGTAGAAAAATTACATGATGCCGTGCTACGCAGACCCAAAGGCAAGCCTCTTATAACGGTCAGCAATCATGTTGCTTCTATGGATGATCCGCTTGTTCTCTCTGCGTTGCTTCCTCGACATGTTCTTCTGGATGCTCAGAACTTGAGGTGGACACTTTGCGCGTCTGATCGTTGTTTTGCAAATCCTGTAACTTCAGCATTTTTTCGATCTGTCAAAGTCCTGCCAGTTTCTCGTGGTGATGGGATTTATCAAAAGGGAATGGATGAGGCTATTTCAAAATTGAATCAAGGTGGTTGGGTTCACATCTTCCCAGAAGGTAGTCGTTCTCGAGATGGTGGAAAAACCATGGGGTCTTCCAAGAGAGGTGTCGGGAGGTTGGTCCTTGACGCCGACAATATTCCCATGGTTGTTCCATTTGTGCATAGCGGGATGCAGGACATTATGCCTATTGGTGCTAGCATCCCAAGGATAGGCAATACGGTAACGGTGGTTATTGGCGATCCAATCTACTTCGATGATTTGTTGAATTCTGAAGGAGCTAAGCATGTATCAAGAGGAAAGCTGTACGATGCTGTATCTTCAAGGATTGGTCATAGACTAAGCGAACTGAAAGTTCAGGTCGACAAATTAGCTCAAGTAACTCGGTCACAAAATCTTCCTGCGCAGAACACAGACCGAGCTGCAGTGATTTTGCAGCATGTTGATTGGGAATCATTCGGAATGGGGAACCTTACATCTTCCGAAGATGATGGTTCACCGGTGCAAGAAACTGAGATCCAACTAGCTGCTCCTCCCCATACGCAAGAACCCCGGTCTGCTGATTGGAGTTACAGAGTGGGTTTCTCCCGTGAAGGTGGCTTTGCATCACGGATGCGTAGTTTCATGGACCAGAGTGAGTTTATGGGTTTCGCAGCCAGAGGCATATTTATGAACCGTAGAGTCCAAGAAACCTCTCCGAGCGGCCGAGGGGTTGGTCCGTTGAAAGCATGGAGACGATACTTGGAGGCCAATGTATTACCACAATGGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0007507 GO:0008150 GO:0008152 GO:0008374 GO:0009987 GO:0016020 GO:0016043 GO:0016740 GO:0016746 GO:0016747 GO:0019637 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0032048 GO:0032501 GO:0032502 GO:0035965 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046471 GO:0046486 GO:0048513 GO:0048731 GO:0048856 GO:0061024 GO:0071704 GO:0071840 GO:0072359

KEGG Pathways

Metabolic & Signaling

ko00564 map00564

Pfam Domains

Protein Families

Acyltransferase

Protein Analysis

463

Amino Acids

51.83

Weight (kDa)

9.83

Isoelectric Point (pI)

49.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyltransferase	PF01553	115 - 259	1.1e-18	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05510 AT3G05510
fragaria_vesca	FvH4_6g06570
malus_domestica	MD04G1200600.v1.1 MD12G1213100.v1.1
prunus_persica	Prupe.2G037700_v2.0.a1 Prupe.6G320700_v2.0.a1 Prupe.6G320700_v2.0.a1
pyrus_communis	pycom04g17680 pycom12g19900
rosa_chinensis	RchiOBHm_Chr1g0322071 RchiOBHm_Chr1g0322081 RchiOBHm_Chr1g0322141 RchiOBHm_Chr3g0455731
rosa_laevigata	RLG00000025335
rosa_multiflora	Rmu_sc0002883.1_g000013
rosa_roxburghii	Rroxscaffold_2G00141120 Rroxscaffold_5G00351290 Rroxscaffold_6G00423510 Rroxscaffold_6G00424010
rosa_rugosa	Rorug03G0009600
rosa_samantha	Rh1AG049300 Rh1AG049900 Rh3AG069200 Rh3BG071700 Rh3CG070000 Rh3DG072400
rosa_wichuraiana	Rw1G004550 Rw3G005590 Rw3G005940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	410
Acc16I	TGCGCA	1 cut(s)	1020
AccB1I	GGYRCC	1 cut(s)	108
AccBSI	CCGCTC	1 cut(s)	1317
AccI	GTMKAC	1 cut(s)	978
AccII	CGCG	2 cut(s)	177, 518
AciI	CCGC	8 cut(s)	76, 100, 184, 210, 220, 446, 783, 1317
AclWI	GGATC	4 cut(s)	102, 437, 851, 1126
AcoI	YGGCCR	1 cut(s)	1318
AcsI	RAATTY	1 cut(s)	883
AcuI	CTGAAG	3 cut(s)	270, 534, 909
AcyI	GRCGYC	1 cut(s)	744
AfaI	GTAC	1 cut(s)	923
AfiI	CCNNNNNNNGG	2 cut(s)	302, 827
AflIII	ACRYGT	1 cut(s)	475
AgeI	ACCGGT	1 cut(s)	1114
AgsI	TTSAA	8 cut(s)	244, 281, 337, 633, 640, 883, 939, 1339
AluBI	AGCT	7 cut(s)	61, 284, 896, 919, 989, 1037, 1142
AluI	AGCT	7 cut(s)	61, 284, 896, 919, 989, 1037, 1142
Alw26I	GTCTC	1 cut(s)	1342
AlwI	GGATC	4 cut(s)	102, 437, 851, 1126
Ama87I	CYCGRG	2 cut(s)	164, 684
AoxI	GGCC	3 cut(s)	45, 1318, 1362
ApeKI	GCWGC	9 cut(s)	5, 8, 58, 73, 154, 1037, 1051, 1142, 1268
ApoI	RAATTY	1 cut(s)	883
AsiGI	ACCGGT	1 cut(s)	1114
AspLEI	GCGC	2 cut(s)	518, 1021
AspS9I	GGNCC	4 cut(s)	46, 736, 1243, 1331
AsuC2I	CCSGG	1 cut(s)	1168
AsuHPI	GGTGA	2 cut(s)	605, 1104
AsuNHI	GCTAGC	1 cut(s)	812
AvaI	CYCGRG	2 cut(s)	164, 684
AvaII	GGWCC	3 cut(s)	736, 1243, 1331
BanI	GGYRCC	1 cut(s)	108
BauI	CACGAG	1 cut(s)	588
BbsI	GAAGAC	1 cut(s)	702
BbvI	GCAGC	7 cut(s)	70, 85, 166, 1024, 1063, 1129, 1280
BccI	CCATC	3 cut(s)	590, 683, 1100
BceAI	ACGGC	2 cut(s)	171, 362
BcnI	CCSGG	1 cut(s)	1168
BcoDI	GTCTC	1 cut(s)	1342
BfaI	CTAG	2 cut(s)	813, 1139
BfmI	CTRYAG	1 cut(s)	1038
BlpI	GCTNAGC	1 cut(s)	897
Bme1390I	CCNGG	1 cut(s)	1168
Bme18I	GGWCC	3 cut(s)	736, 1243, 1331
BmeT110I	CYCGRG	2 cut(s)	164, 684
BmgT120I	GGNCC	4 cut(s)	46, 736, 1243, 1331
BmiI	GGNNCC	2 cut(s)	110, 1085
BmrFI	CCNGG	1 cut(s)	1168
BmsI	GCATC	7 cut(s)	364, 478, 777, 825, 916, 1218, 1229
BmtI	GCTAGC	1 cut(s)	816
BpiI	GAAGAC	1 cut(s)	702
Bpu10I	CCTNAGC	1 cut(s)	68
Bpu1102I	GCTNAGC	1 cut(s)	897
BpuEI	CTTGAG	2 cut(s)	520, 975
BpuMI	CCSGG	1 cut(s)	1168
BsaHI	GRCGYC	1 cut(s)	744
BsaJI	CCNNGG	5 cut(s)	702, 759, 821, 1166, 1321
BsaWI	WCCGGW	2 cut(s)	295, 1114
BsaXI	ACNNNNNCTCC	2 cut(s)	128, 158
Bsc4I	CCNNNNNNNGG	2 cut(s)	302, 827
Bse118I	RCCGGY	1 cut(s)	1114
Bse1I	ACTGG	1 cut(s)	581
Bse3DI	GCAATG	2 cut(s)	149, 313
BseDI	CCNNGG	5 cut(s)	702, 759, 821, 1166, 1321
BseGI	GGATG	6 cut(s)	445, 493, 625, 792, 816, 1233
BseLI	CCNNNNNNNGG	2 cut(s)	302, 827
BseMI	GCAATG	2 cut(s)	149, 313
BseMII	CTCAG	3 cut(s)	82, 506, 1119
BseNI	ACTGG	1 cut(s)	581
BseRI	GAGGAG	2 cut(s)	133, 1137
BseX3I	CGGCCG	1 cut(s)	1318
BseXI	GCAGC	7 cut(s)	70, 85, 166, 1024, 1063, 1129, 1280
BsgI	GTGCAG	1 cut(s)	77
Bsh1236I	CGCG	2 cut(s)	177, 518
Bsh1285I	CGRYCG	1 cut(s)	1321
BshFI	GGCC	3 cut(s)	47, 1320, 1364
BshNI	GGYRCC	1 cut(s)	108
BshTI	ACCGGT	1 cut(s)	1114
BsiEI	CGRYCG	1 cut(s)	1321
BsiHKCI	CYCGRG	2 cut(s)	164, 684
BsiSI	CCGG	5 cut(s)	64, 106, 296, 1115, 1168
BslI	CCNNNNNNNGG	2 cut(s)	302, 827
BsmAI	GTCTC	1 cut(s)	1342
BsmBI	CGTCTC	1 cut(s)	1342
BsnI	GGCC	3 cut(s)	47, 1320, 1364
BsoBI	CYCGRG	2 cut(s)	164, 684
Bsp143I	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
Bsp1720I	GCTNAGC	1 cut(s)	897
Bsp19I	CCATGG	2 cut(s)	702, 759
Bsp68I	TCGCGA	1 cut(s)	177
BspACI	CCGC	8 cut(s)	76, 100, 184, 210, 220, 446, 783, 1317
BspANI	GGCC	3 cut(s)	47, 1320, 1364
BspCNI	CTCAG	3 cut(s)	81, 505, 1120
BspFNI	CGCG	2 cut(s)	177, 518
BspLI	GGNNCC	2 cut(s)	110, 1085
BspMAI	CTGCAG	1 cut(s)	1042
BspOI	GCTAGC	1 cut(s)	816
BspPI	GGATC	4 cut(s)	102, 437, 851, 1126
BspQI	GCTCTTC	1 cut(s)	32
BspT107I	GGYRCC	1 cut(s)	108
BsrBI	CCGCTC	1 cut(s)	1317
BsrDI	GCAATG	2 cut(s)	149, 313
BsrFI	RCCGGY	1 cut(s)	1114
BsrI	ACTGG	1 cut(s)	581
BssAI	RCCGGY	1 cut(s)	1114
BssECI	CCNNGG	5 cut(s)	702, 759, 821, 1166, 1321
BssMI	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
BssNI	GRCGYC	1 cut(s)	744
BssSI	CACGAG	1 cut(s)	588
BssT1I	CCWWGG	3 cut(s)	702, 759, 821
Bst2BI	CACGAG	1 cut(s)	588
Bst4CI	ACNGT	5 cut(s)	134, 415, 838, 844, 1292
Bst6I	CTCTTC	1 cut(s)	32
BstACI	GRCGYC	1 cut(s)	744
BstC8I	GCNNGC	3 cut(s)	275, 401, 814
BstDEI	CTNAG	5 cut(s)	68, 492, 897, 956, 1128
BstDSI	CCRYGG	2 cut(s)	702, 759
BstF5I	GGATG	6 cut(s)	445, 493, 625, 792, 816, 1233
BstFNI	CGCG	2 cut(s)	177, 518
BstHHI	GCGC	2 cut(s)	518, 1021
BstKTI	GATC	7 cut(s)	31, 97, 445, 526, 566, 859, 1134
BstMAI	GTCTC	1 cut(s)	1342
BstMBI	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
BstMCI	CGRYCG	1 cut(s)	1321
BstMWI	GCNNNNNNNGC	4 cut(s)	64, 73, 160, 183
BstNSI	RCATGY	4 cut(s)	277, 479, 905, 1058
BstSCI	CCNGG	1 cut(s)	1166
BstSFI	CTRYAG	1 cut(s)	1038
BstUI	CGCG	2 cut(s)	177, 518
BstV1I	GCAGC	7 cut(s)	70, 85, 166, 1024, 1063, 1129, 1280
BstV2I	GAAGAC	1 cut(s)	702
BstX2I	RGATCY	1 cut(s)	1131
BstYI	RGATCY	1 cut(s)	1131
BstZI	CGGCCG	1 cut(s)	1318
BsuRI	GGCC	3 cut(s)	47, 1320, 1364
BtgI	CCRYGG	2 cut(s)	702, 759
BtsCI	GGATG	6 cut(s)	445, 493, 625, 792, 816, 1233
BtsI	GCAGTG	1 cut(s)	1047
BtsIMutI	CAGTG	1 cut(s)	1047
BtuMI	TCGCGA	1 cut(s)	177
Cac8I	GCNNGC	3 cut(s)	275, 401, 814
CfoI	GCGC	2 cut(s)	518, 1021
Cfr10I	RCCGGY	1 cut(s)	1114
Cfr13I	GGNCC	4 cut(s)	46, 736, 1243, 1331
CseI	GACGC	2 cut(s)	507, 752
Csp6I	GTAC	1 cut(s)	922
CspAI	ACCGGT	1 cut(s)	1114
CviQI	GTAC	1 cut(s)	922
DdeI	CTNAG	5 cut(s)	68, 492, 897, 956, 1128
DpnI	GATC	7 cut(s)	30, 96, 444, 525, 565, 858, 1133
DpnII	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
EaeI	YGGCCR	1 cut(s)	1318
EagI	CGGCCG	1 cut(s)	1318
Eam1104I	CTCTTC	1 cut(s)	32
EarI	CTCTTC	1 cut(s)	32
EciI	GGCGGA	1 cut(s)	173
EclXI	CGGCCG	1 cut(s)	1318
Eco130I	CCWWGG	3 cut(s)	702, 759, 821
Eco47I	GGWCC	3 cut(s)	736, 1243, 1331
Eco52I	CGGCCG	1 cut(s)	1318
Eco57I	CTGAAG	3 cut(s)	270, 534, 909
Eco88I	CYCGRG	2 cut(s)	164, 684
EcoO109I	RGGNCCY	1 cut(s)	46
EcoRI	GAATTC	1 cut(s)	883
EcoT14I	CCWWGG	3 cut(s)	702, 759, 821
ErhI	CCWWGG	3 cut(s)	702, 759, 821
Esp3I	CGTCTC	1 cut(s)	1342
FauI	CCCGC	1 cut(s)	776
FblI	GTMKAC	1 cut(s)	978
FokI	GGATG	6 cut(s)	452, 500, 632, 799, 803, 1240
FspBI	CTAG	2 cut(s)	813, 1139
FspI	TGCGCA	1 cut(s)	1020
GlaI	GCGC	2 cut(s)	517, 1020
HaeIII	GGCC	3 cut(s)	47, 1320, 1364
HapII	CCGG	5 cut(s)	64, 106, 296, 1115, 1168
HgaI	GACGC	2 cut(s)	507, 752
HhaI	GCGC	2 cut(s)	518, 1021
Hin1I	GRCGYC	1 cut(s)	744
Hin6I	GCGC	2 cut(s)	516, 1019
HinP1I	GCGC	2 cut(s)	516, 1019
HincII	GTYRAC	1 cut(s)	979
HindII	GTYRAC	1 cut(s)	979
HinfI	GANTC	6 cut(s)	191, 257, 337, 640, 1067, 1296
HpaII	CCGG	5 cut(s)	64, 106, 296, 1115, 1168
HphI	GGTGA	2 cut(s)	605, 1104
Hpy166II	GTNNAC	4 cut(s)	507, 658, 979, 1112
Hpy188I	TCNGA	6 cut(s)	495, 523, 889, 1076, 1099, 1314
Hpy188III	TCNNGA	6 cut(s)	176, 485, 684, 686, 727, 909
Hpy8I	GTNNAC	4 cut(s)	507, 658, 979, 1112
Hpy99I	CGWCG	1 cut(s)	134
HpyAV	CCTTC	6 cut(s)	210, 223, 275, 665, 884, 1202
HpyCH4III	ACNGT	5 cut(s)	134, 415, 838, 844, 1292
HpyCH4V	TGCA	9 cut(s)	58, 154, 536, 778, 790, 1040, 1051, 1120, 1220
HpyF10VI	GCNNNNNNNGC	4 cut(s)	64, 73, 160, 183
HpyF3I	CTNAG	5 cut(s)	68, 492, 897, 956, 1128
Hsp92I	GRCGYC	1 cut(s)	744
HspAI	GCGC	2 cut(s)	516, 1019
Kzo9I	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
LguI	GCTCTTC	1 cut(s)	32
LmnI	GCTCC	3 cut(s)	66, 893, 1150
Lsp1109I	GCAGC	7 cut(s)	70, 85, 166, 1024, 1063, 1129, 1280
LweI	GCATC	7 cut(s)	364, 478, 777, 825, 916, 1218, 1229
MaeI	CTAG	2 cut(s)	813, 1139
MaeIII	GTNAC	6 cut(s)	134, 544, 838, 994, 1002, 1184
MalI	GATC	7 cut(s)	30, 96, 444, 525, 565, 858, 1133
MbiI	CCGCTC	1 cut(s)	1317
MboI	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
MflI	RGATCY	1 cut(s)	1131
MluCI	AATT	5 cut(s)	365, 635, 883, 983, 1384
MlyI	GAGTC	1 cut(s)	1305
MmeI	TCCRAC	2 cut(s)	150, 1159
MslI	CAYNNNNRTG	1 cut(s)	1283
MspA1I	CMGCKG	1 cut(s)	76
MspI	CCGG	5 cut(s)	64, 106, 296, 1115, 1168
MspR9I	CCNGG	1 cut(s)	1168
MvnI	CGCG	2 cut(s)	177, 518
MwoI	GCNNNNNNNGC	4 cut(s)	64, 73, 160, 183
NciI	CCSGG	1 cut(s)	1168
NcoI	CCATGG	2 cut(s)	702, 759
NdeII	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
NheI	GCTAGC	1 cut(s)	812
NlaIV	GGNNCC	2 cut(s)	110, 1085
NmeAIII	GCCGAG	1 cut(s)	1346
NmuCI	GTSAC	1 cut(s)	1002
NruI	TCGCGA	1 cut(s)	177
NsbI	TGCGCA	1 cut(s)	1020
NspI	RCATGY	4 cut(s)	277, 479, 905, 1058
PaeI	GCATGC	1 cut(s)	277
PaeR7I	CTCGAG	2 cut(s)	164, 684
PciI	ACATGT	1 cut(s)	475
PciSI	GCTCTTC	1 cut(s)	32
PfeI	GAWTC	5 cut(s)	191, 257, 337, 640, 1067
PinAI	ACCGGT	1 cut(s)	1114
PleI	GAGTC	1 cut(s)	1304
PpsI	GAGTC	1 cut(s)	1304
PscI	ACATGT	1 cut(s)	475
PsiI	TTATAA	1 cut(s)	410
PspN4I	GGNNCC	2 cut(s)	110, 1085
PspPI	GGNCC	4 cut(s)	46, 736, 1243, 1331
PspXI	VCTCGAGB	1 cut(s)	164
PstI	CTGCAG	1 cut(s)	1042
PsuI	RGATCY	1 cut(s)	1131
RruI	TCGCGA	1 cut(s)	177
RsaI	GTAC	1 cut(s)	923
RsaNI	GTAC	1 cut(s)	922
RseI	CAYNNNNRTG	1 cut(s)	1283
SalI	GTCGAC	1 cut(s)	977
SapI	GCTCTTC	1 cut(s)	32
Sau3AI	GATC	7 cut(s)	28, 94, 442, 523, 563, 856, 1131
Sau96I	GGNCC	4 cut(s)	46, 736, 1243, 1331
SchI	GAGTC	1 cut(s)	1305
ScrFI	CCNGG	1 cut(s)	1168
SfaNI	GCATC	7 cut(s)	364, 478, 777, 825, 916, 1218, 1229
SfcI	CTRYAG	1 cut(s)	1038
Sfr274I	CTCGAG	2 cut(s)	164, 684
SgrAI	CRCCGGYG	1 cut(s)	1114
SinI	GGWCC	3 cut(s)	736, 1243, 1331
SlaI	CTCGAG	2 cut(s)	164, 684
SmiMI	CAYNNNNRTG	1 cut(s)	1283
SmlI	CTYRAG	4 cut(s)	164, 499, 684, 990
SmoI	CTYRAG	4 cut(s)	164, 499, 684, 990
SphI	GCATGC	1 cut(s)	277
Sse9I	AATT	5 cut(s)	365, 635, 883, 983, 1384
SsiI	CCGC	8 cut(s)	76, 100, 184, 210, 220, 446, 783, 1317
SspI	AATATT	1 cut(s)	754
SspMI	CTAG	2 cut(s)	813, 1139
StyD4I	CCNGG	1 cut(s)	1166
StyI	CCWWGG	3 cut(s)	702, 759, 821
TaaI	ACNGT	5 cut(s)	134, 415, 838, 844, 1292
TaqI	TCGA	6 cut(s)	165, 472, 562, 685, 870, 978
TaqII	GACCGA	2 cut(s)	990, 1047
TasI	AATT	5 cut(s)	365, 635, 883, 983, 1384
TauI	GCSGC	2 cut(s)	102, 1320
TfiI	GAWTC	5 cut(s)	191, 257, 337, 640, 1067
TscAI	CASTG	1 cut(s)	1047
TseFI	GTSAC	1 cut(s)	1002
TseI	GCWGC	9 cut(s)	5, 8, 58, 73, 154, 1037, 1051, 1142, 1268
Tsp45I	GTSAC	1 cut(s)	1002
TspDTI	ATGAA	3 cut(s)	341, 1228, 1301
TspGWI	ACGGA	2 cut(s)	1240, 1323
TspRI	CASTG	1 cut(s)	1047
VpaK11BI	GGWCC	3 cut(s)	736, 1243, 1331
XapI	RAATTY	1 cut(s)	883
XceI	RCATGY	4 cut(s)	277, 479, 905, 1058
XhoI	CTCGAG	2 cut(s)	164, 684
XmiI	GTMKAC	1 cut(s)	978
XspI	CTAG	2 cut(s)	813, 1139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.