Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0005072

Physical Location & Seq

Genomic Coordinates Reverse (-)

45253596 .. 45260990

Gene Structure 7395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00424010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGGCTGCTGCGAGGGTTGAGCGCGGAGATCTGTGGAAGAGCAATGCCCTCTCCGTACAACGCCGGCTCAGGGAGCGGTTCAGAGTCGAGGTGGACCGCCGGTTCAGGCACCACCCGGTTTTCGCTGACGGTTACTTCGCCTCCACCGTGCAGCGTTGGCTCCAGCGGTTCCGTGATTTCAAGAGGGACTCGCTTCCGTCCTCCACCGTCTTCTATCGCAAACGAGTGGGCAAGGAAATCAACGCTGAAGAAGAGTCGGCCATTCTTCGCATGGTGCAAGCTGTTGCTGTGCCGGTCATTGGCAATGTTTGCCATGTGTTCATGAATGGATTAAATCGTGTTCAGGTCTATGGTTTAGAAAAATTACATGACGCATTGCTACGCAGACCCAAAGACAGGCCTCTTATAACGGTTAGCAATCATGTGGCTTCTATGGATGATCCGCTTGTTATTGCTGCACTGCTTCCTCCAAATGTTCTTCTGGATGCTAAGAACTTGAGGTGGACACTCTGTGCATCTGATAGGTGTTTTTCGAATCCTGTAACTTCAGCATTTTTCCGATCTGTCAAAGTCTTGCCAGTTGCTCGTGGTGATGGGATTTATCAAAAGGGTATGGACATTGCTATTTCAAAATTAAATCAAGGTGGCTGGGTTCACATCTTCCCAGAAGGTAGTCGTTCTCGAGATGGTGGTAAAACCCTGGGGTCACCTAAGAGAGGTGTTGGGAGGTTGGTCCTTGACGCTGACAATATTCCCATGGTTATTCCATTTGTACATAGCGGGATGCAGGACATCATGCCTATTGGTGCCAGCTTCCCGAGGATTGGCAAGACGGTGACGGTGGTTATTGGTGATCCAATCTACTTTGATGATTTACTAAATAATGAAGGAGCAAAGCATTTATCAAGAGGAAAGTTATATGATGCAGTATCCTCAAGAATCGGTCATCGACTACGTCAACTGAAAGTTCAGGTTGACAAACTGACTCAAGGGACACAATTACTAAATGATGCCAGAGGCTTATTTACGAACCGTAGAGCAGGGATCAACCAAACGGCACCATGTGAAGGCGAGCTCCGTGGGAATGAGTGTGCTTTGGATGGAGAAATACTTAGATATGTGGGTGAAGCTTATCCAAAAGGAGTCTGCTCAGTGTACTGTGGGAATGGTAAAGATGCAGAGGGCCCAGGATCTGACTTTGAGCTCGTGGTAGTGATTTCAGCTGCTAGACATAGCCCACAAAACTTCTGGTCTATGAAACTTAATGCATTAGATTTACTCCTGCAGGCATCCTATTCAAACTTGCCCGATACCACTTTTAAGGATCTTCGTAGAAAGCTTCCAGTTACTCGCACCCTATTTCCATGGCAGAATACTTTACAGTTTAGCTTGACAAGAGATATCACAAAAGAACTTGGCATTGGAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0007275 GO:0007507 GO:0008150 GO:0008152 GO:0008374 GO:0009987 GO:0016020 GO:0016043 GO:0016740 GO:0016746 GO:0016747 GO:0019637 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0032048 GO:0032501 GO:0032502 GO:0035965 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046471 GO:0046486 GO:0048513 GO:0048731 GO:0048856 GO:0061024 GO:0071704 GO:0071840 GO:0072359

KEGG Pathways

Metabolic & Signaling

ko00564 map00564

Pfam Domains

Protein Families

Acyltransferase

Protein Analysis

476

Amino Acids

53.23

Weight (kDa)

9.78

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Acyltransferase	PF01553	113 - 258	6.2e-18	Acyltransferase
F-actin_cap_A	PF01267	354 - 418	1.8e-06	F-actin capping protein alpha subunit
F-actin_cap_A	PF01267	427 - 467	1.4e-09	F-actin capping protein alpha subunit

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05510 AT3G05510
fragaria_vesca	FvH4_6g06570
malus_domestica	MD04G1200600.v1.1 MD12G1213100.v1.1
prunus_persica	Prupe.2G037700_v2.0.a1 Prupe.6G320700_v2.0.a1 Prupe.6G320700_v2.0.a1
pyrus_communis	pycom04g17680 pycom12g19900
rosa_chinensis	RchiOBHm_Chr1g0322071 RchiOBHm_Chr1g0322081 RchiOBHm_Chr1g0322141 RchiOBHm_Chr3g0455731
rosa_laevigata	RLG00000025335
rosa_multiflora	Rmu_sc0002883.1_g000013
rosa_roxburghii	Rroxscaffold_2G00141120 Rroxscaffold_5G00351290 Rroxscaffold_6G00423510 Rroxscaffold_6G00424010
rosa_rugosa	Rorug03G0009600
rosa_samantha	Rh1AG049300 Rh1AG049900 Rh3AG069200 Rh3BG071700 Rh3CG070000 Rh3DG072400
rosa_wichuraiana	Rw1G004550 Rw3G005590 Rw3G005940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	407
AccB1I	GGYRCC	3 cut(s)	108, 806, 1057
AccBSI	CCGCTC	1 cut(s)	76
AccII	CGCG	1 cut(s)	24
AciI	CCGC	6 cut(s)	24, 76, 97, 166, 443, 780
AclWI	GGATC	5 cut(s)	434, 848, 1052, 1198, 1332
AcoI	YGGCCR	1 cut(s)	258
AcuI	CTGAAG	2 cut(s)	267, 531
AdeI	CACNNNGTG	2 cut(s)	512, 1064
AfaI	GTAC	3 cut(s)	57, 774, 1157
AfiI	CCNNNNNNNGG	6 cut(s)	70, 299, 396, 716, 824, 1067
AgsI	TTSAA	3 cut(s)	181, 630, 1299
AjnI	CCWGG	2 cut(s)	699, 1186
AjuI	GAANNNNNNNTTGG	1 cut(s)	1404
AloI	GAACNNNNNNTCC	2 cut(s)	86, 118
AluBI	AGCT	8 cut(s)	281, 813, 1075, 1130, 1204, 1223, 1339, 1389
AluI	AGCT	8 cut(s)	281, 813, 1075, 1130, 1204, 1223, 1339, 1389
Alw21I	GWGCWC	2 cut(s)	1077, 1206
AlwI	GGATC	5 cut(s)	434, 848, 1052, 1198, 1332
AlwNI	CAGNNNCTG	1 cut(s)	1193
Ama87I	CYCGRG	2 cut(s)	681, 817
AoxI	GGCC	3 cut(s)	258, 398, 1183
ApaI	GGGCCC	1 cut(s)	1187
ApeKI	GCWGC	5 cut(s)	5, 8, 151, 455, 1223
ArsI	GACNNNNNNTTYG	2 cut(s)	119, 151
AspLEI	GCGC	1 cut(s)	24
AspS9I	GGNCC	4 cut(s)	94, 733, 1183, 1184
AsuC2I	CCSGG	1 cut(s)	116
AsuHPI	GGTGA	5 cut(s)	602, 699, 847, 863, 1136
AsuII	TTCGAA	1 cut(s)	533
AvaI	CYCGRG	2 cut(s)	681, 817
AvaII	GGWCC	2 cut(s)	94, 733
BaeGI	GKGCMC	1 cut(s)	1187
BanI	GGYRCC	3 cut(s)	108, 806, 1057
BanII	GRGCYC	3 cut(s)	1077, 1187, 1206
BauI	CACGAG	2 cut(s)	585, 1205
BbsI	GAAGAC	1 cut(s)	202
Bbv12I	GWGCWC	2 cut(s)	1077, 1206
BbvI	GCAGC	3 cut(s)	163, 442, 1210
BccI	CCATC	3 cut(s)	587, 680, 1094
BceAI	ACGGC	1 cut(s)	1071
BciT130I	CCWGG	2 cut(s)	701, 1188
BciVI	GTATCC	1 cut(s)	940
BcnI	CCSGG	1 cut(s)	116
BfaI	CTAG	1 cut(s)	1227
BfmI	CTRYAG	1 cut(s)	1283
BfuI	GTATCC	1 cut(s)	940
BglII	AGATCT	1 cut(s)	28
BisI	GCNGC	5 cut(s)	6, 9, 152, 456, 1224
BlsI	GCNGC	5 cut(s)	7, 10, 153, 457, 1225
Bme1390I	CCNGG	3 cut(s)	116, 701, 1188
Bme18I	GGWCC	2 cut(s)	94, 733
BmeT110I	CYCGRG	2 cut(s)	681, 817
BmgT120I	GGNCC	4 cut(s)	94, 733, 1183, 1184
BmiI	GGNNCC	6 cut(s)	110, 161, 170, 808, 1059, 1185
BmrFI	CCNGG	3 cut(s)	116, 701, 1188
BmsI	GCATC	7 cut(s)	475, 524, 774, 913, 1000, 1165, 1298
BpiI	GAAGAC	1 cut(s)	202
BpmI	CTGGAG	1 cut(s)	146
Bpu10I	CCTNAGC	1 cut(s)	68
Bpu14I	TTCGAA	1 cut(s)	533
BpuEI	CTTGAG	3 cut(s)	517, 919, 972
BpuMI	CCSGG	1 cut(s)	116
BsaJI	CCNNGG	7 cut(s)	699, 700, 756, 818, 1078, 1186, 1364
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	6 cut(s)	70, 299, 396, 716, 824, 1067
Bse118I	RCCGGY	3 cut(s)	63, 99, 292
Bse1I	ACTGG	2 cut(s)	578, 1343
Bse3DI	GCAATG	4 cut(s)	49, 310, 374, 618
BseBI	CCWGG	2 cut(s)	701, 1188
BseDI	CCNNGG	7 cut(s)	699, 700, 756, 818, 1078, 1186, 1364
BseGI	GGATG	5 cut(s)	442, 490, 789, 1105, 1289
BseLI	CCNNNNNNNGG	6 cut(s)	70, 299, 396, 716, 824, 1067
BseMI	GCAATG	4 cut(s)	49, 310, 374, 618
BseMII	CTCAG	2 cut(s)	82, 1164
BseNI	ACTGG	2 cut(s)	578, 1343
BseSI	GKGCMC	1 cut(s)	1187
BseXI	GCAGC	3 cut(s)	163, 442, 1210
BseYI	CCCAGC	1 cut(s)	648
BsgI	GTGCAG	2 cut(s)	170, 441
Bsh1236I	CGCG	1 cut(s)	24
BshFI	GGCC	3 cut(s)	260, 400, 1185
BshNI	GGYRCC	3 cut(s)	108, 806, 1057
BsiHKAI	GWGCWC	2 cut(s)	1077, 1206
BsiHKCI	CYCGRG	2 cut(s)	681, 817
BsiSI	CCGG	4 cut(s)	64, 100, 116, 293
BslFI	GGGAC	2 cut(s)	200, 1006
BslI	CCNNNNNNNGG	6 cut(s)	70, 299, 396, 716, 824, 1067
BsmFI	GGGAC	2 cut(s)	200, 1006
BsnI	GGCC	3 cut(s)	260, 400, 1185
BsoBI	CYCGRG	2 cut(s)	681, 817
Bsp119I	TTCGAA	1 cut(s)	533
Bsp120I	GGGCCC	1 cut(s)	1183
Bsp1286I	GDGCHC	3 cut(s)	1077, 1187, 1206
Bsp1407I	TGTACA	1 cut(s)	772
Bsp143I	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
Bsp19I	CCATGG	2 cut(s)	756, 1364
BspACI	CCGC	6 cut(s)	24, 76, 97, 166, 443, 780
BspANI	GGCC	3 cut(s)	260, 400, 1185
BspCNI	CTCAG	2 cut(s)	81, 1163
BspFNI	CGCG	1 cut(s)	24
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	6 cut(s)	110, 161, 170, 808, 1059, 1185
BspMAI	CTGCAG	1 cut(s)	1287
BspPI	GGATC	5 cut(s)	434, 848, 1052, 1198, 1332
BspQI	GCTCTTC	1 cut(s)	32
BspT104I	TTCGAA	1 cut(s)	533
BspT107I	GGYRCC	3 cut(s)	108, 806, 1057
BsrBI	CCGCTC	1 cut(s)	76
BsrDI	GCAATG	4 cut(s)	49, 310, 374, 618
BsrFI	RCCGGY	3 cut(s)	63, 99, 292
BsrGI	TGTACA	1 cut(s)	772
BsrI	ACTGG	2 cut(s)	578, 1343
BssAI	RCCGGY	3 cut(s)	63, 99, 292
BssECI	CCNNGG	7 cut(s)	699, 700, 756, 818, 1078, 1186, 1364
BssMI	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
BssSI	CACGAG	2 cut(s)	585, 1205
BssT1I	CCWWGG	2 cut(s)	756, 1364
Bst2BI	CACGAG	2 cut(s)	585, 1205
Bst2UI	CCWGG	2 cut(s)	701, 1188
Bst4CI	ACNGT	9 cut(s)	131, 148, 208, 412, 835, 841, 1034, 1160, 1383
Bst6I	CTCTTC	2 cut(s)	32, 246
BstAPI	GCANNNNNTGC	1 cut(s)	309
BstAUI	TGTACA	1 cut(s)	772
BstBI	TTCGAA	1 cut(s)	533
BstC8I	GCNNGC	5 cut(s)	65, 279, 811, 1073, 1287
BstDEI	CTNAG	5 cut(s)	68, 489, 711, 1112, 1150
BstDSI	CCRYGG	3 cut(s)	756, 1078, 1364
BstEII	GGTNACC	1 cut(s)	705
BstENI	CCTNNNNNAGG	1 cut(s)	714
BstF5I	GGATG	5 cut(s)	442, 490, 789, 1105, 1289
BstFNI	CGCG	1 cut(s)	24
BstHHI	GCGC	1 cut(s)	24
BstKTI	GATC	7 cut(s)	31, 442, 563, 856, 1047, 1193, 1327
BstMBI	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
BstMWI	GCNNNNNNNGC	3 cut(s)	73, 157, 309
BstNI	CCWGG	2 cut(s)	701, 1188
BstPI	GGTNACC	1 cut(s)	705
BstSCI	CCNGG	3 cut(s)	114, 699, 1186
BstSFI	CTRYAG	1 cut(s)	1283
BstSLI	GKGCMC	1 cut(s)	1187
BstUI	CGCG	1 cut(s)	24
BstV1I	GCAGC	3 cut(s)	163, 442, 1210
BstV2I	GAAGAC	1 cut(s)	202
BstX2I	RGATCY	3 cut(s)	28, 1190, 1324
BstYI	RGATCY	3 cut(s)	28, 1190, 1324
BsuI	GTATCC	1 cut(s)	940
BsuRI	GGCC	3 cut(s)	260, 400, 1185
BtgI	CCRYGG	3 cut(s)	756, 1078, 1364
BtsCI	GGATG	5 cut(s)	442, 490, 789, 1105, 1289
BtsI	GCAGTG	1 cut(s)	458
BtsIMutI	CAGTG	2 cut(s)	458, 1158
Cac8I	GCNNGC	5 cut(s)	65, 279, 811, 1073, 1287
CaiI	CAGNNNCTG	1 cut(s)	1193
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	1 cut(s)	24
Cfr10I	RCCGGY	3 cut(s)	63, 99, 292
Cfr13I	GGNCC	4 cut(s)	94, 733, 1183, 1184
CseI	GACGC	2 cut(s)	380, 749
Csp6I	GTAC	3 cut(s)	56, 773, 1156
CviAII	CATG	9 cut(s)	271, 314, 322, 368, 422, 757, 796, 1062, 1365
CviQI	GTAC	3 cut(s)	56, 773, 1156
DdeI	CTNAG	5 cut(s)	68, 489, 711, 1112, 1150
DpnI	GATC	7 cut(s)	30, 441, 562, 855, 1046, 1192, 1326
DpnII	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
DraIII	CACNNNGTG	2 cut(s)	512, 1064
EaeI	YGGCCR	1 cut(s)	258
Eam1104I	CTCTTC	2 cut(s)	32, 246
EarI	CTCTTC	2 cut(s)	32, 246
Ecl136II	GAGCTC	2 cut(s)	1075, 1204
Eco130I	CCWWGG	2 cut(s)	756, 1364
Eco147I	AGGCCT	1 cut(s)	400
Eco24I	GRGCYC	3 cut(s)	1077, 1187, 1206
Eco32I	GATATC	1 cut(s)	1402
Eco47I	GGWCC	2 cut(s)	94, 733
Eco53kI	GAGCTC	2 cut(s)	1075, 1204
Eco57I	CTGAAG	2 cut(s)	267, 531
Eco88I	CYCGRG	2 cut(s)	681, 817
Eco91I	GGTNACC	1 cut(s)	705
EcoICRI	GAGCTC	2 cut(s)	1075, 1204
EcoNI	CCTNNNNNAGG	1 cut(s)	714
EcoO109I	RGGNCCY	1 cut(s)	1183
EcoO65I	GGTNACC	1 cut(s)	705
EcoRII	CCWGG	2 cut(s)	699, 1186
EcoRV	GATATC	1 cut(s)	1402
EcoT14I	CCWWGG	2 cut(s)	756, 1364
EcoT22I	ATGCAT	1 cut(s)	1270
EcoT38I	GRGCYC	3 cut(s)	1077, 1187, 1206
ErhI	CCWWGG	2 cut(s)	756, 1364
FaeI	CATG	9 cut(s)	274, 317, 325, 371, 425, 760, 799, 1065, 1368
FaqI	GGGAC	2 cut(s)	200, 1006
FatI	CATG	9 cut(s)	270, 313, 321, 367, 421, 756, 795, 1061, 1364
FauI	CCCGC	1 cut(s)	773
Fnu4HI	GCNGC	5 cut(s)	6, 9, 152, 456, 1224
FokI	GGATG	5 cut(s)	449, 497, 796, 1112, 1276
FriOI	GRGCYC	3 cut(s)	1077, 1187, 1206
Fsp4HI	GCNGC	5 cut(s)	6, 9, 152, 456, 1224
FspBI	CTAG	1 cut(s)	1227
GlaI	GCGC	1 cut(s)	23
GluI	GCNGC	5 cut(s)	6, 9, 152, 456, 1224
GsaI	CCCAGC	1 cut(s)	652
GsuI	CTGGAG	1 cut(s)	146
HaeIII	GGCC	3 cut(s)	260, 400, 1185
HapII	CCGG	4 cut(s)	64, 100, 116, 293
HgaI	GACGC	2 cut(s)	380, 749
HhaI	GCGC	1 cut(s)	24
Hin1II	CATG	9 cut(s)	274, 317, 325, 371, 425, 760, 799, 1065, 1368
Hin6I	GCGC	1 cut(s)	22
HinP1I	GCGC	1 cut(s)	22
HincII	GTYRAC	2 cut(s)	959, 976
HindII	GTYRAC	2 cut(s)	959, 976
HindIII	AAGCTT	2 cut(s)	1128, 1337
HinfI	GANTC	7 cut(s)	84, 188, 254, 535, 939, 985, 1143
HpaII	CCGG	4 cut(s)	64, 100, 116, 293
HphI	GGTGA	5 cut(s)	602, 699, 847, 863, 1136
Hpy166II	GTNNAC	6 cut(s)	94, 504, 655, 959, 976, 1156
Hpy188I	TCNGA	4 cut(s)	83, 520, 560, 1195
Hpy188III	TCNNGA	8 cut(s)	181, 322, 482, 681, 683, 817, 906, 936
Hpy8I	GTNNAC	6 cut(s)	94, 504, 655, 959, 976, 1156
HpyAV	CCTTC	3 cut(s)	662, 881, 1061
HpyCH4III	ACNGT	9 cut(s)	131, 148, 208, 412, 835, 841, 1034, 1160, 1383
HpyCH4IV	ACGT	1 cut(s)	955
HpyCH4V	TGCA	9 cut(s)	151, 277, 458, 515, 787, 926, 1178, 1268, 1285
HpyF10VI	GCNNNNNNNGC	3 cut(s)	73, 157, 309
HpyF3I	CTNAG	5 cut(s)	68, 489, 711, 1112, 1150
HpySE526I	ACGT	1 cut(s)	955
Hsp92II	CATG	9 cut(s)	274, 317, 325, 371, 425, 760, 799, 1065, 1368
HspAI	GCGC	1 cut(s)	22
KroI	GCCGGC	1 cut(s)	63
KroNI	GCCGGC	1 cut(s)	65
Kzo9I	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
LguI	GCTCTTC	1 cut(s)	32
LmnI	GCTCC	4 cut(s)	73, 165, 890, 1080
Lsp1109I	GCAGC	3 cut(s)	163, 442, 1210
LweI	GCATC	7 cut(s)	475, 524, 774, 913, 1000, 1165, 1298
MaeI	CTAG	1 cut(s)	1227
MaeII	ACGT	1 cut(s)	955
MaeIII	GTNAC	5 cut(s)	131, 541, 705, 835, 1345
MalI	GATC	7 cut(s)	30, 441, 562, 855, 1046, 1192, 1326
MbiI	CCGCTC	1 cut(s)	76
MboI	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
MboII	GAAGA	8 cut(s)	49, 202, 257, 260, 263, 470, 652, 1319
MflI	RGATCY	3 cut(s)	28, 1190, 1324
MhlI	GDGCHC	3 cut(s)	1077, 1187, 1206
MluCI	AATT	3 cut(s)	362, 632, 998
MlyI	GAGTC	5 cut(s)	93, 182, 263, 979, 1152
Mph1103I	ATGCAT	1 cut(s)	1270
MroNI	GCCGGC	1 cut(s)	63
MseI	TTAA	4 cut(s)	332, 635, 1263, 1320
MspA1I	CMGCKG	2 cut(s)	166, 1223
MspI	CCGG	4 cut(s)	64, 100, 116, 293
MspR9I	CCNGG	3 cut(s)	116, 701, 1188
MvaI	CCWGG	2 cut(s)	701, 1188
MvnI	CGCG	1 cut(s)	24
MwoI	GCNNNNNNNGC	3 cut(s)	73, 157, 309
NaeI	GCCGGC	1 cut(s)	65
NciI	CCSGG	1 cut(s)	116
NcoI	CCATGG	2 cut(s)	756, 1364
NdeII	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
NgoMIV	GCCGGC	1 cut(s)	63
NlaIII	CATG	9 cut(s)	274, 317, 325, 371, 425, 760, 799, 1065, 1368
NlaIV	GGNNCC	6 cut(s)	110, 161, 170, 808, 1059, 1185
NmuCI	GTSAC	2 cut(s)	705, 835
NsiI	ATGCAT	1 cut(s)	1270
NspV	TTCGAA	1 cut(s)	533
PaeR7I	CTCGAG	1 cut(s)	681
PagI	TCATGA	1 cut(s)	321
PasI	CCCWGGG	1 cut(s)	700
PceI	AGGCCT	1 cut(s)	400
PciSI	GCTCTTC	1 cut(s)	32
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PdiI	GCCGGC	1 cut(s)	65
PfeI	GAWTC	2 cut(s)	535, 939
PflFI	GACNNNGTC	1 cut(s)	954
PkrI	GCNGC	5 cut(s)	7, 10, 153, 457, 1225
PleI	GAGTC	5 cut(s)	92, 182, 262, 979, 1151
PpsI	GAGTC	5 cut(s)	92, 182, 262, 979, 1151
PsiI	TTATAA	1 cut(s)	407
Psp124BI	GAGCTC	2 cut(s)	1077, 1206
Psp6I	CCWGG	2 cut(s)	699, 1186
PspEI	GGTNACC	1 cut(s)	705
PspFI	CCCAGC	1 cut(s)	648
PspGI	CCWGG	2 cut(s)	699, 1186
PspN4I	GGNNCC	6 cut(s)	110, 161, 170, 808, 1059, 1185
PspOMI	GGGCCC	1 cut(s)	1183
PspPI	GGNCC	4 cut(s)	94, 733, 1183, 1184
PstI	CTGCAG	1 cut(s)	1287
PstNI	CAGNNNCTG	1 cut(s)	1193
PsuI	RGATCY	3 cut(s)	28, 1190, 1324
PsyI	GACNNNGTC	1 cut(s)	954
PvuII	CAGCTG	1 cut(s)	1223
RsaI	GTAC	3 cut(s)	57, 774, 1157
RsaNI	GTAC	3 cut(s)	56, 773, 1156
SacI	GAGCTC	2 cut(s)	1077, 1206
SapI	GCTCTTC	1 cut(s)	32
SaqAI	TTAA	4 cut(s)	332, 635, 1263, 1320
SatI	GCNGC	5 cut(s)	6, 9, 152, 456, 1224
Sau3AI	GATC	7 cut(s)	28, 439, 560, 853, 1044, 1190, 1324
Sau96I	GGNCC	4 cut(s)	94, 733, 1183, 1184
SbfI	CCTGCAGG	1 cut(s)	1287
SchI	GAGTC	5 cut(s)	93, 182, 263, 979, 1152
ScrFI	CCNGG	3 cut(s)	116, 701, 1188
SdaI	CCTGCAGG	1 cut(s)	1287
SduI	GDGCHC	3 cut(s)	1077, 1187, 1206
SfaNI	GCATC	7 cut(s)	475, 524, 774, 913, 1000, 1165, 1298
SfcI	CTRYAG	1 cut(s)	1283
Sfr274I	CTCGAG	1 cut(s)	681
SfuI	TTCGAA	1 cut(s)	533
SinI	GGWCC	2 cut(s)	94, 733
SlaI	CTCGAG	1 cut(s)	681
SmlI	CTYRAG	4 cut(s)	496, 681, 934, 987
SmoI	CTYRAG	4 cut(s)	496, 681, 934, 987
Sse8387I	CCTGCAGG	1 cut(s)	1287
Sse9I	AATT	3 cut(s)	362, 632, 998
SseBI	AGGCCT	1 cut(s)	400
SsiI	CCGC	6 cut(s)	24, 76, 97, 166, 443, 780
SspI	AATATT	1 cut(s)	751
SspMI	CTAG	1 cut(s)	1227
SstI	GAGCTC	2 cut(s)	1077, 1206
StuI	AGGCCT	1 cut(s)	400
StyD4I	CCNGG	3 cut(s)	114, 699, 1186
StyI	CCWWGG	2 cut(s)	756, 1364
TaaI	ACNGT	9 cut(s)	131, 148, 208, 412, 835, 841, 1034, 1160, 1383
TaiI	ACGT	1 cut(s)	958
TaqI	TCGA	4 cut(s)	87, 533, 682, 949
TaqII	GACCGA	1 cut(s)	932
TasI	AATT	3 cut(s)	362, 632, 998
TatI	WGTACW	2 cut(s)	772, 1155
TfiI	GAWTC	2 cut(s)	535, 939
Tru1I	TTAA	4 cut(s)	332, 635, 1263, 1320
Tru9I	TTAA	4 cut(s)	332, 635, 1263, 1320
TscAI	CASTG	2 cut(s)	465, 1158
TseFI	GTSAC	2 cut(s)	705, 835
TseI	GCWGC	5 cut(s)	5, 8, 151, 455, 1223
Tsp45I	GTSAC	2 cut(s)	705, 835
TspDTI	ATGAA	4 cut(s)	310, 338, 900, 1271
TspGWI	ACGGA	4 cut(s)	43, 161, 186, 1067
TspRI	CASTG	2 cut(s)	465, 1158
Tth111I	GACNNNGTC	1 cut(s)	954
VpaK11BI	GGWCC	2 cut(s)	94, 733
XagI	CCTNNNNNAGG	1 cut(s)	714
XcmI	CCANNNNNNNNNTGG	2 cut(s)	268, 1245
XhoI	CTCGAG	1 cut(s)	681
XspI	CTAG	1 cut(s)	1227
Zsp2I	ATGCAT	1 cut(s)	1270

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.