MD04G1215200.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005149
Physical Location & Seq
Forward (+)
29828186 .. 29836059
7874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1215200.v1.1.491

Sequence Viewer

Length: 2952 bp
ATGTGCATATGCTGCCCTGCTATGAGGTCGAGATCTAGACAACCTGTTAAGCGTTACAAAAAGCTGCTTGCGGAAATCTTTCCCAAGGCTCCTGATGGCCCTCCCAATGAAAGAAAAATTGTTAAGTTATGTGAATATGCTGCCAAAAACCCTTTTCGAATCCCAAAGATTGCTAAATATCTTGAAGATAGGTGCTACAAAGAGCTTAGACTGGGGCACATCAAATTCATCAATATTGTTGCAGACACTTACAACAAGTTGCTTTGCCTGTGTAAGGAGCAGATGGCATGTTTTGCAGTCAGTCTGGTGAGTGTGGTTACTGAGCTCCTGGACAACTCTAAGCAAGATACTGTGCGCATACTTGGATGCCAAACTCTAACTAATTTCATCTATAGTCAGACAGACTGCACTTACACGCATACCATTGAGAGTTTGGTTCACAAAGTATGTAAGCTTGCACGTCAAAGTGGTGAAGATCAACAAAGCTTGAGAGCATCAAGTTTGCAGTGCCTTTCAGCCATGGTGAAATTCATGGCAGAATTTTCATATATTTTTGTTGATTTTGATGAGATTATTCATGTCACCTTGGATAACTATGAGCCAGATACACATAATGAAGATGATGAGAGAGGGCAGCTACATCATAATTGGGTGGATGAAGTGGTTCGAAGTGAAAGCAGAGTTGGTGTAGTTGGTGATGATGCTAGTCCTAGGTTCAAGATCATCAGGCCGCGACCAGAAAAGAAGGATCCTGCTCTTCTGACAAGAGAAGAGATTGAAACACCAAAAGTGTGGGCTCAAATTTGTATTCAGAGAATGATAGAGTTATCCAAGGAGAGCACAACGATGCGCCGAGTATTGGATCCAATGTTTGTCTACTTTGATTCTGGGCACCACTGGGTTCCTCGGCAAGGGTTGGCCATGCTGGTTTTGTCTGATATGTCATACTTTATGGAGGCTTCAGGGAATCAACAGTTGATTTTAACCTATGTGATCCGTCATCTTGACCACAAAAACATTTCACAAGATCCACAACTCAAATCTTCTGTCATTCAAGTTGCTAGTGCTTTGGCTAGCCAGATTCGATCTGGTGCAGTGTTGGCAGAAATTGGATTTGTCTCTGACCTATGTAGGCATTTGAGGAAGAGTCTTCAAGCCACTGCTGAATCAGTTGGAGAGCAAGAATCAAACATTAATATCATGCTACAAAATTCCATTGAAGACTGCTTACTAGAAATTTCGAGAGGCATTGGCAATGTTCGGCCACTATTTGACATGATGGCGATAACTCTGGAGAATCTGCCATCTGGAATTGTTGCTAGGGCAACACTTGGATCTTTAATGATTGTTGCTCATATGATCTCATTAGCGTTGATCTCTTCACGTACACAGCAGGTGTTTCCTGAGTCCCTTCTTGTCCAACTTCTGAAAGTCATGGTGCATCCAGATGTTGAGGTTCGTGTTGGAGCACACCAGATATTTTCTATTCTTCTCATTCCAACTTCTAACCAGCCCCGGCACGAATCTTCATCTTTACAATCTGGTTTTGGATACCAATCAAGAGGATGGCAGTCGAATACAGCATCTACCTTTGCATCAATCACAGCAAGACTTGAAAAGCTGAGGAAGGAAAAAGATGGCCCCAAAGCCGAAAAGCACGGGACCAGTACTGCTTGTGATGATTTTAGAGATAGAGAGACTGCTGAAGAAGTCTGGAAGCAGGGGTGTAACCGAAAAAATTCCCCTAATGTTTACAAAATTAGCTCAATTATTGACAGGACGGCTGGAACAGTCAGCTTAACAGAGCCGGATCCGTATGTTATGAAGTTTAATGAGGATCAAATATCACACTTGCTGTCTGCTCTTTGGATACAAACAAATCTCTTGGATAATTTGCCTTCAAATATTGAGGCTATAGCTCATTCTTTTACTTTAGTGCTTATCTCTTCACATTTGAAGAATCCAACTGACAACCTTATGGTTCGCTTCTTTCAACTTCTGCTGTCTCTGAGGAATGTATCCCTTGATTCTAACAATGGGATGTTGCCTCCAGCATGTCAAAGATCTATGCTTGTACTCTCGATTGGCATGTTGATGTTTGCGGCTAAGATCTATCACATTCCTCTTCTCAAGTCATTTATACCGTATGATATTCAGGTTGATCCATATTTAGGCATCAGTGACGATCTTCAAATTTATGTAAAGCCTGATGCAGATGTTAGTAAATATGGATCTGTTACTGATAACGAGATGGCTTCATCGTTTCTATCTGATTTGCGGAACAAAATATACGAATCAGATAATATCATGATGGAAATCTTAGTTCAGTTTCTATCAAAGGTTTGTGAGATGAAGGCAGAAGATGTGGCGAACCAACTTTCAGAGTCATTCACACCTGATGATGCATTCATGTTTGGCCCACAGTCAATGCTTGACTGTGACCAAAATCAAATGCCAGTCCATTCGAAGGAATCATCATTTGATGGGGAATTTCCAACAAATTCATCAGTGGAGGATGATGTGACAAGTGAAGCATCTGTTGCTGACCTCTCACGTTTCATTCCCAGAGTACCTACATCCTCCTCTATACCGCCCCATGTTATCAGCATTGGACAGCTGATAGAGTCGGCATTTGAGGTAGCTGGTCAAGTAGCAGGGACAGCTGTCTCTACATCACCTCTCCCCTACAACACCATGGCAAGCCAGTGCGAAGCCCTTGGCACAGGGACAAGGAAGAAACTCTCCAACTGGTTGGCCCATGAAAATCATCAGAGCAGTGTTCGTGACAGATTGTTTCCAGCATTTCCTGCAGATGGACGCGCAGCACTCCAGAAGATAACGAGTGACATTGGGCCTGCTCATGGAGCTGCATCGGCACAGGATCCCTGGTTGGCTATGAGGCTGCCTCCTGCTAGTCCATTTGACAACTTCCTCAAGGCAGCTGGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

984

Amino Acids

109.81

Weight (kDa)

5.89

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 51 - 144 6.5e-21 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1384
Acc16I TGCGCA 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 1384
AccB1I GGYRCC 1 cut(s) 891
AccI GTMKAC 1 cut(s) 876
AccII CGCG 2 cut(s) 733, 2820
AciI CCGC 5 cut(s) 71, 731, 2102, 2278, 2591
AcoI YGGCCR 2 cut(s) 918, 1262
AcuI CTGAAG 2 cut(s) 945, 1725
AfaI GTAC 4 cut(s) 1387, 1669, 2076, 2571
AfiI CCNNNNNNNGG 7 cut(s) 274, 859, 911, 2171, 2467, 2813, 2861
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 2 cut(s) 327, 2885
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
Alw21I GWGCWC 3 cut(s) 327, 842, 1471
Alw26I GTCTC 4 cut(s) 1123, 1691, 2010, 2671
AoxI GGCC 8 cut(s) 97, 728, 918, 1262, 1639, 2416, 2754, 2852
ApeKI GCWGC 8 cut(s) 12, 64, 140, 634, 2822, 2867, 2902, 2939
ArsI GACNNNNNNTTYG 2 cut(s) 1032, 1064
AseI ATTAAT 1 cut(s) 1194
Asp700I GAANNNNTTC 3 cut(s) 78, 663, 1738
AspA2I CCTAGG 1 cut(s) 710
AspLEI GCGC 3 cut(s) 357, 852, 2822
AspS9I GGNCC 6 cut(s) 98, 1640, 1662, 2417, 2755, 2852
AsuC2I CCSGG 1 cut(s) 1516
AsuHPI GGTGA 6 cut(s) 319, 482, 535, 574, 707, 2667
AsuII TTCGAA 3 cut(s) 157, 667, 2465
AsuNHI GCTAGC 1 cut(s) 1073
AvaII GGWCC 1 cut(s) 1662
AvrII CCTAGG 1 cut(s) 710
BaeGI GKGCMC 2 cut(s) 219, 894
BalI TGGCCA 1 cut(s) 920
BamHI GGATCC 4 cut(s) 748, 862, 1810, 2881
BanI GGYRCC 1 cut(s) 891
BanII GRGCYC 2 cut(s) 327, 799
BarI GAAGNNNNNNTAC 2 cut(s) 1212, 1244
BbsI GAAGAC 2 cut(s) 1142, 1227
Bbv12I GWGCWC 3 cut(s) 327, 842, 1471
BbvCI CCTCAGC 1 cut(s) 1622
BbvI GCAGC 6 cut(s) 51, 127, 646, 2834, 2854, 2889
BceAI ACGGC 1 cut(s) 1797
BciT130I CCWGG 2 cut(s) 329, 2887
BciVI GTATCC 3 cut(s) 1544, 1863, 2029
BcnI CCSGG 1 cut(s) 1516
BcoDI GTCTC 4 cut(s) 1123, 1691, 2010, 2671
BfaI CTAG 8 cut(s) 36, 705, 711, 1062, 1074, 1232, 1320, 2913
BfmI CTRYAG 3 cut(s) 391, 1914, 2808
BfuAI ACCTGC 1 cut(s) 1384
BfuI GTATCC 3 cut(s) 1544, 1863, 2029
BglII AGATCT 3 cut(s) 32, 2063, 2109
BlnI CCTAGG 1 cut(s) 710
BmcAI AGTACT 1 cut(s) 1669
Bme1390I CCNGG 3 cut(s) 329, 1516, 2887
Bme18I GGWCC 1 cut(s) 1662
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 6 cut(s) 98, 1640, 1662, 2417, 2755, 2852
BmiI GGNNCC 9 cut(s) 90, 750, 864, 893, 903, 1642, 1663, 1812, 2883
BmrFI CCNGG 3 cut(s) 329, 1516, 2887
BmrI ACTGGG 2 cut(s) 221, 907
BmtI GCTAGC 1 cut(s) 1077
BmuI ACTGGG 2 cut(s) 221, 907
BoxI GACNNNNGTC 1 cut(s) 2663
BpiI GAAGAC 2 cut(s) 1142, 1227
BplI GAGNNNNNCTC 2 cut(s) 2890, 2922
BpmI CTGGAG 3 cut(s) 1313, 2034, 2813
Bpu10I CCTNAGC 1 cut(s) 1622
Bpu14I TTCGAA 3 cut(s) 157, 667, 2465
BpuEI CTTGAG 3 cut(s) 508, 2114, 2918
BpuMI CCSGG 1 cut(s) 1516
BsaAI YACGTR 1 cut(s) 1385
BsaBI GATNNNNATC 2 cut(s) 1555, 2315
Bsc4I CCNNNNNNNGG 7 cut(s) 274, 859, 911, 2171, 2467, 2813, 2861
Bse1I ACTGG 6 cut(s) 216, 902, 1665, 2456, 2704, 2753
Bse3DI GCAATG 1 cut(s) 1261
Bse8I GATNNNNATC 2 cut(s) 1555, 2315
BseBI CCWGG 2 cut(s) 329, 2887
BseGI GGATG 7 cut(s) 371, 661, 1441, 1571, 2046, 2521, 2576
BseJI GATNNNNATC 2 cut(s) 1555, 2315
BseLI CCNNNNNNNGG 7 cut(s) 274, 859, 911, 2171, 2467, 2813, 2861
BseMI GCAATG 1 cut(s) 1261
BseMII CTCAG 4 cut(s) 312, 1395, 1613, 2000
BseNI ACTGG 6 cut(s) 216, 902, 1665, 2456, 2704, 2753
BseRI GAGGAG 1 cut(s) 2572
BseSI GKGCMC 2 cut(s) 219, 894
BseXI GCAGC 6 cut(s) 51, 127, 646, 2834, 2854, 2889
BseYI CCCAGC 1 cut(s) 2942
BsgI GTGCAG 2 cut(s) 391, 1113
Bsh1236I CGCG 2 cut(s) 733, 2820
BshFI GGCC 8 cut(s) 99, 730, 920, 1264, 1641, 2418, 2756, 2854
BshNI GGYRCC 1 cut(s) 891
BsiHKAI GWGCWC 3 cut(s) 327, 842, 1471
BsiSI CCGG 2 cut(s) 1516, 1808
BslFI GGGAC 4 cut(s) 1393, 1675, 2671, 2740
BslI CCNNNNNNNGG 7 cut(s) 274, 859, 911, 2171, 2467, 2813, 2861
BsmAI GTCTC 4 cut(s) 1123, 1691, 2010, 2671
BsmFI GGGAC 4 cut(s) 1393, 1675, 2671, 2740
BsmI GAATGC 1 cut(s) 2405
BsnI GGCC 8 cut(s) 99, 730, 920, 1264, 1641, 2418, 2756, 2854
Bsp119I TTCGAA 3 cut(s) 157, 667, 2465
Bsp1286I GDGCHC 6 cut(s) 219, 327, 799, 842, 894, 1471
Bsp19I CCATGG 2 cut(s) 519, 2694
BspACI CCGC 5 cut(s) 71, 731, 2102, 2278, 2591
BspANI GGCC 8 cut(s) 99, 730, 920, 1264, 1641, 2418, 2756, 2854
BspCNI CTCAG 4 cut(s) 313, 1396, 1614, 2001
BspFNI CGCG 2 cut(s) 733, 2820
BspHI TCATGA 1 cut(s) 2307
BspLI GGNNCC 9 cut(s) 90, 750, 864, 893, 903, 1642, 1663, 1812, 2883
BspMAI CTGCAG 1 cut(s) 2812
BspMI ACCTGC 1 cut(s) 1384
BspOI GCTAGC 1 cut(s) 1077
BspQI GCTCTTC 1 cut(s) 762
BspT104I TTCGAA 3 cut(s) 157, 667, 2465
BspT107I GGYRCC 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 1261
BsrI ACTGG 6 cut(s) 216, 902, 1665, 2456, 2704, 2753
BssT1I CCWWGG 7 cut(s) 84, 519, 585, 710, 831, 2694, 2716
Bst2UI CCWGG 2 cut(s) 329, 2887
Bst4CI ACNGT 6 cut(s) 352, 975, 1792, 2145, 2424, 2438
Bst6I CTCTTC 6 cut(s) 762, 765, 1139, 1384, 1951, 2130
BstAPI GCANNNNNTGC 4 cut(s) 12, 293, 2540, 2807
BstBAI YACGTR 1 cut(s) 1385
BstBI TTCGAA 3 cut(s) 157, 667, 2465
BstC8I GCNNGC 5 cut(s) 69, 456, 1075, 2701, 2856
BstDEI CTNAG 8 cut(s) 206, 321, 339, 1404, 1622, 2009, 2106, 2320
BstDSI CCRYGG 2 cut(s) 519, 2694
BstENI CCTNNNNNAGG 2 cut(s) 272, 909
BstF5I GGATG 7 cut(s) 371, 661, 1441, 1571, 2046, 2521, 2576
BstFNI CGCG 2 cut(s) 733, 2820
BstHHI GCGC 3 cut(s) 357, 852, 2822
BstMAI GTCTC 4 cut(s) 1123, 1691, 2010, 2671
BstMWI GCNNNNNNNGC 8 cut(s) 12, 293, 1100, 2540, 2660, 2807, 2864, 2873
BstNI CCWGG 2 cut(s) 329, 2887
BstNSI RCATGY 3 cut(s) 291, 2058, 2092
BstPAI GACNNNNGTC 1 cut(s) 2663
BstSCI CCNGG 3 cut(s) 327, 1514, 2885
BstSFI CTRYAG 3 cut(s) 391, 1914, 2808
BstSLI GKGCMC 2 cut(s) 219, 894
BstUI CGCG 2 cut(s) 733, 2820
BstV1I GCAGC 6 cut(s) 51, 127, 646, 2834, 2854, 2889
BstV2I GAAGAC 2 cut(s) 1142, 1227
BstXI CCANNNNNNTGG 2 cut(s) 792, 2752
BsuI GTATCC 3 cut(s) 1544, 1863, 2029
BsuRI GGCC 8 cut(s) 99, 730, 920, 1264, 1641, 2418, 2756, 2854
BtgI CCRYGG 2 cut(s) 519, 2694
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 7 cut(s) 371, 661, 1441, 1571, 2046, 2521, 2576
BtsI GCAGTG 4 cut(s) 512, 1101, 1158, 2782
BtsIMutI CAGTG 8 cut(s) 512, 895, 1101, 1158, 2185, 2514, 2711, 2782
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 5 cut(s) 69, 456, 1075, 2701, 2856
CciI TCATGA 1 cut(s) 2307
CfoI GCGC 3 cut(s) 357, 852, 2822
Cfr13I GGNCC 6 cut(s) 98, 1640, 1662, 2417, 2755, 2852
CseI GACGC 1 cut(s) 2826
Csp6I GTAC 4 cut(s) 1386, 1668, 2075, 2570
CviQI GTAC 4 cut(s) 1386, 1668, 2075, 2570
DdeI CTNAG 8 cut(s) 206, 321, 339, 1404, 1622, 2009, 2106, 2320
EaeI YGGCCR 2 cut(s) 918, 1262
Eam1104I CTCTTC 6 cut(s) 762, 765, 1139, 1384, 1951, 2130
EarI CTCTTC 6 cut(s) 762, 765, 1139, 1384, 1951, 2130
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 7 cut(s) 84, 519, 585, 710, 831, 2694, 2716
Eco24I GRGCYC 2 cut(s) 327, 799
Eco47I GGWCC 1 cut(s) 1662
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 2 cut(s) 945, 1725
EcoICRI GAGCTC 1 cut(s) 325
EcoNI CCTNNNNNAGG 2 cut(s) 272, 909
EcoRII CCWGG 2 cut(s) 327, 2885
EcoT14I CCWWGG 7 cut(s) 84, 519, 585, 710, 831, 2694, 2716
EcoT22I ATGCAT 1 cut(s) 2407
EcoT38I GRGCYC 2 cut(s) 327, 799
ErhI CCWWGG 7 cut(s) 84, 519, 585, 710, 831, 2694, 2716
FaqI GGGAC 4 cut(s) 1393, 1675, 2671, 2740
FauNDI CATATG 2 cut(s) 8, 1356
FblI GTMKAC 1 cut(s) 876
FokI GGATG 7 cut(s) 378, 668, 1428, 1578, 2053, 2528, 2563
FriOI GRGCYC 2 cut(s) 327, 799
FspAI RTGCGCAY 1 cut(s) 356
FspBI CTAG 8 cut(s) 36, 705, 711, 1062, 1074, 1232, 1320, 2913
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 3 cut(s) 356, 851, 2821
GsaI CCCAGC 1 cut(s) 2946
GsuI CTGGAG 3 cut(s) 1313, 2034, 2813
HaeIII GGCC 8 cut(s) 99, 730, 920, 1264, 1641, 2418, 2756, 2854
HapII CCGG 2 cut(s) 1516, 1808
HgaI GACGC 1 cut(s) 2826
HhaI GCGC 3 cut(s) 357, 852, 2822
Hin6I GCGC 3 cut(s) 355, 850, 2820
HinP1I GCGC 3 cut(s) 355, 850, 2820
HindIII AAGCTT 2 cut(s) 452, 484
HpaII CCGG 2 cut(s) 1516, 1808
HphI GGTGA 6 cut(s) 319, 482, 535, 574, 707, 2667
Hpy166II GTNNAC 4 cut(s) 439, 877, 1388, 1753
Hpy8I GTNNAC 4 cut(s) 439, 877, 1388, 1753
HpyAV CCTTC 6 cut(s) 739, 1421, 1621, 1908, 2347, 2461
HpyCH4III ACNGT 6 cut(s) 352, 975, 1792, 2145, 2424, 2438
HpyCH4IV ACGT 3 cut(s) 460, 1384, 2554
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 293, 1100, 2540, 2660, 2807, 2864, 2873
HpyF3I CTNAG 8 cut(s) 206, 321, 339, 1404, 1622, 2009, 2106, 2320
HpySE526I ACGT 3 cut(s) 460, 1384, 2554
HspAI GCGC 3 cut(s) 355, 850, 2820
LguI GCTCTTC 1 cut(s) 762
LmnI GCTCC 5 cut(s) 94, 277, 330, 1466, 2864
Lsp1109I GCAGC 6 cut(s) 51, 127, 646, 2834, 2854, 2889
MaeI CTAG 8 cut(s) 36, 705, 711, 1062, 1074, 1232, 1320, 2913
MaeII ACGT 3 cut(s) 460, 1384, 2554
MhlI GDGCHC 6 cut(s) 219, 327, 799, 842, 894, 1471
MlsI TGGCCA 1 cut(s) 920
MluNI TGGCCA 1 cut(s) 920
MlyI GAGTC 4 cut(s) 1156, 1415, 2393, 2633
MmeI TCCRAC 7 cut(s) 1153, 1444, 1444, 1523, 1988, 2519, 2769
Mox20I TGGCCA 1 cut(s) 920
Mph1103I ATGCAT 1 cut(s) 2407
MroXI GAANNNNTTC 3 cut(s) 78, 663, 1738
MscI TGGCCA 1 cut(s) 920
MseI TTAA 7 cut(s) 48, 123, 983, 1194, 1340, 1799, 1830
MslI CAYNNNNRTG 3 cut(s) 845, 1446, 2093
Msp20I TGGCCA 1 cut(s) 920
MspA1I CMGCKG 3 cut(s) 2617, 2663, 2942
MspI CCGG 2 cut(s) 1516, 1808
MspR9I CCNGG 3 cut(s) 329, 1516, 2887
Mva1269I GAATGC 1 cut(s) 2405
MvaI CCWGG 2 cut(s) 329, 2887
MvnI CGCG 2 cut(s) 733, 2820
MwoI GCNNNNNNNGC 8 cut(s) 12, 293, 1100, 2540, 2660, 2807, 2864, 2873
NciI CCSGG 1 cut(s) 1516
NcoI CCATGG 2 cut(s) 519, 2694
NdeI CATATG 2 cut(s) 8, 1356
NheI GCTAGC 1 cut(s) 1073
NlaIV GGNNCC 9 cut(s) 90, 750, 864, 893, 903, 1642, 1663, 1812, 2883
NmeAIII GCCGAG 2 cut(s) 878, 886
NmuCI GTSAC 6 cut(s) 580, 2180, 2438, 2521, 2783, 2843
NsbI TGCGCA 1 cut(s) 356
NsiI ATGCAT 1 cut(s) 2407
NspI RCATGY 3 cut(s) 291, 2058, 2092
NspV TTCGAA 3 cut(s) 157, 667, 2465
PagI TCATGA 1 cut(s) 2307
PaqCI CACCTGC 1 cut(s) 1384
PciSI GCTCTTC 1 cut(s) 762
PctI GAATGC 1 cut(s) 2405
PdmI GAANNNNTTC 3 cut(s) 78, 663, 1738
PfoI TCCNGGA 1 cut(s) 327
PleI GAGTC 4 cut(s) 1155, 1414, 2392, 2632
PpsI GAGTC 4 cut(s) 1155, 1414, 2392, 2632
Ppu21I YACGTR 1 cut(s) 1385
PshAI GACNNNNGTC 1 cut(s) 2663
PshBI ATTAAT 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 2 cut(s) 327, 2885
PspFI CCCAGC 1 cut(s) 2942
PspGI CCWGG 2 cut(s) 327, 2885
PspN4I GGNNCC 9 cut(s) 90, 750, 864, 893, 903, 1642, 1663, 1812, 2883
PspPI GGNCC 6 cut(s) 98, 1640, 1662, 2417, 2755, 2852
PsrI GAACNNNNNNTAC 2 cut(s) 2273, 2305
PstI CTGCAG 1 cut(s) 2812
PvuII CAGCTG 3 cut(s) 2617, 2663, 2942
RsaI GTAC 4 cut(s) 1387, 1669, 2076, 2571
RsaNI GTAC 4 cut(s) 1386, 1668, 2075, 2570
RseI CAYNNNNRTG 3 cut(s) 845, 1446, 2093
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 7 cut(s) 48, 123, 983, 1194, 1340, 1799, 1830
Sau96I GGNCC 6 cut(s) 98, 1640, 1662, 2417, 2755, 2852
ScaI AGTACT 1 cut(s) 1669
SchI GAGTC 4 cut(s) 1156, 1415, 2393, 2633
ScrFI CCNGG 3 cut(s) 329, 1516, 2887
SduI GDGCHC 6 cut(s) 219, 327, 799, 842, 894, 1471
SfcI CTRYAG 3 cut(s) 391, 1914, 2808
SfuI TTCGAA 3 cut(s) 157, 667, 2465
SinI GGWCC 1 cut(s) 1662
SmiMI CAYNNNNRTG 3 cut(s) 845, 1446, 2093
SmlI CTYRAG 3 cut(s) 487, 2129, 2933
SmoI CTYRAG 3 cut(s) 487, 2129, 2933
SsiI CCGC 5 cut(s) 71, 731, 2102, 2278, 2591
SspI AATATT 2 cut(s) 235, 1906
SspMI CTAG 8 cut(s) 36, 705, 711, 1062, 1074, 1232, 1320, 2913
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 3 cut(s) 327, 1514, 2885
StyI CCWWGG 7 cut(s) 84, 519, 585, 710, 831, 2694, 2716
TaaI ACNGT 6 cut(s) 352, 975, 1792, 2145, 2424, 2438
TaiI ACGT 3 cut(s) 463, 1387, 2557
TaqI TCGA 8 cut(s) 29, 157, 667, 1084, 1241, 1574, 2081, 2465
TatI WGTACW 2 cut(s) 1667, 2074
TauI GCSGC 2 cut(s) 733, 2105
Tru1I TTAA 7 cut(s) 48, 123, 983, 1194, 1340, 1799, 1830
Tru9I TTAA 7 cut(s) 48, 123, 983, 1194, 1340, 1799, 1830
TscAI CASTG 8 cut(s) 512, 902, 1101, 1165, 2185, 2514, 2711, 2782
TseFI GTSAC 6 cut(s) 580, 2180, 2438, 2521, 2783, 2843
TseI GCWGC 8 cut(s) 12, 64, 140, 634, 2822, 2867, 2902, 2939
Tsp45I GTSAC 6 cut(s) 580, 2180, 2438, 2521, 2783, 2843
TspGWI ACGGA 2 cut(s) 986, 1803
TspRI CASTG 8 cut(s) 512, 902, 1101, 1165, 2185, 2514, 2711, 2782
VpaK11BI GGWCC 1 cut(s) 1662
VspI ATTAAT 1 cut(s) 1194
XagI CCTNNNNNAGG 2 cut(s) 272, 909
XbaI TCTAGA 1 cut(s) 35
XceI RCATGY 3 cut(s) 291, 2058, 2092
XcmI CCANNNNNNNNNTGG 2 cut(s) 430, 1085
XmaJI CCTAGG 1 cut(s) 710
XmiI GTMKAC 1 cut(s) 876
XmnI GAANNNNTTC 3 cut(s) 78, 663, 1738
XspI CTAG 8 cut(s) 36, 705, 711, 1062, 1074, 1232, 1320, 2913
ZrmI AGTACT 1 cut(s) 1669
Zsp2I ATGCAT 1 cut(s) 2407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.