pycom12g19350

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005149
Physical Location & Seq
Forward (+)
21219409 .. 21221814
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19350.6

Sequence Viewer

Length: 738 bp
ATGTGCATATGCTGCCCTGCTATGAGGTCAAGATCTAGACAACCCGTTAAGCGTTACAAAAAGCTGCTTGCCGAAATCTTTCCCAAGTCTCCTGATGGCCCTCCCAATGAAAGAAAAATTGTCAAGTTATGCGAATATGCTGCCAAAAATCCTTTTCGAATCCCAAAGATTGCCAAATATCTTGAAGATAGATGCTACAAAGAGCTTAGATTGGAGCACATCAAATTCATCAATATTGTTGTGGAGGCTTACAACAAGTTGCTTTGCCTGTGTAAGGAGCAGATGGCATGTTTTGCAGTCAGTCTGGTGCATGTGGTCACTGAGCTCCTGGACAACTCTAAGCAAGATGTTATGCGCATACTTGGATGCCAAGCCCTAACGAATTTCATCTATAGTCAGACAGACGGCACTTACACACATACCATTGAGAGTTTGGTTCACAAAGTATGTAAGCTTGCACGTCAAAGTGGTGAAGTTCATCAAAGCTTGAGAGCATCAAGTTTGCAATGCCTTTCAGCCATGGTGCGATTCATGGCAGAATTTTCATATATTTTTGTTGATTTTGATGAGATTGTACATGTCACCTTGGATAACTATGAGCCAGATACACATAATGAAGATGATGAGAGAGGGCAGCCACATCATAATTGGGTGGATGAAGTGGTTCGAAGTGAAAGCAGAGTTGGTGTAGTTGGCGATGACGCTAGCCCTAGCTGCAAGATCGATCTCACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

28.1

Weight (kDa)

6.75

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 356
AcsI RAATTY 3 cut(s) 224, 382, 539
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 1 cut(s) 274
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 1 cut(s) 185
AjiI CACGTC 1 cut(s) 461
AjnI CCWGG 1 cut(s) 327
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AluBI AGCT 6 cut(s) 64, 205, 325, 454, 486, 714
AluI AGCT 6 cut(s) 64, 205, 325, 454, 486, 714
Alw21I GWGCWC 2 cut(s) 219, 327
Alw26I GTCTC 1 cut(s) 93
AoxI GGCC 1 cut(s) 97
ApeKI GCWGC 5 cut(s) 12, 64, 140, 634, 714
ApoI RAATTY 3 cut(s) 224, 382, 539
Asp700I GAANNNNTTC 2 cut(s) 78, 663
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 482, 574
AsuII TTCGAA 2 cut(s) 157, 667
AsuNHI GCTAGC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 327
Bbv12I GWGCWC 2 cut(s) 219, 327
BbvI GCAGC 4 cut(s) 51, 127, 646, 701
BccI CCATC 2 cut(s) 89, 277
BceAI ACGGC 1 cut(s) 421
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 1 cut(s) 329
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 3 cut(s) 36, 705, 711
BfmI CTRYAG 1 cut(s) 391
BglII AGATCT 1 cut(s) 32
BisI GCNGC 5 cut(s) 13, 65, 141, 635, 715
BlsI GCNGC 5 cut(s) 14, 66, 142, 636, 716
Bme1390I CCNGG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 98
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 3 cut(s) 182, 356, 503
BmtI GCTAGC 1 cut(s) 708
Bpu14I TTCGAA 2 cut(s) 157, 667
BpuEI CTTGAG 1 cut(s) 508
Bsa29I ATCGAT 1 cut(s) 723
BsaJI CCNNGG 2 cut(s) 519, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 512
BseBI CCWGG 1 cut(s) 329
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 519, 585
BseGI GGATG 2 cut(s) 371, 661
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 312
BseXI GCAGC 4 cut(s) 51, 127, 646, 701
BshFI GGCC 1 cut(s) 99
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 2 cut(s) 219, 327
BslI CCNNNNNNNGG 1 cut(s) 274
BsmAI GTCTC 1 cut(s) 93
BsnI GGCC 1 cut(s) 99
Bsp119I TTCGAA 2 cut(s) 157, 667
Bsp1286I GDGCHC 2 cut(s) 219, 327
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 3 cut(s) 32, 720, 724
Bsp19I CCATGG 1 cut(s) 519
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 313
BspDI ATCGAT 1 cut(s) 723
BspOI GCTAGC 1 cut(s) 708
BspT104I TTCGAA 2 cut(s) 157, 667
BsrDI GCAATG 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 574
BssECI CCNNGG 2 cut(s) 519, 585
BssMI GATC 3 cut(s) 32, 720, 724
BssT1I CCWWGG 2 cut(s) 519, 585
Bst2UI CCWGG 1 cut(s) 329
BstAPI GCANNNNNTGC 2 cut(s) 12, 293
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 2 cut(s) 157, 667
BstC8I GCNNGC 3 cut(s) 69, 456, 706
BstDEI CTNAG 3 cut(s) 206, 321, 339
BstDSI CCRYGG 1 cut(s) 519
BstENI CCTNNNNNAGG 1 cut(s) 272
BstF5I GGATG 2 cut(s) 371, 661
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 3 cut(s) 35, 723, 727
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 3 cut(s) 32, 720, 724
BstMWI GCNNNNNNNGC 3 cut(s) 12, 293, 714
BstNI CCWGG 1 cut(s) 329
BstNSI RCATGY 3 cut(s) 291, 314, 581
BstSCI CCNGG 1 cut(s) 327
BstSFI CTRYAG 1 cut(s) 391
BstV1I GCAGC 4 cut(s) 51, 127, 646, 701
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
Bsu15I ATCGAT 1 cut(s) 723
BsuRI GGCC 1 cut(s) 99
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 519
BtgZI GCGATG 1 cut(s) 711
BtrI CACGTC 1 cut(s) 461
BtsCI GGATG 2 cut(s) 371, 661
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 3 cut(s) 69, 456, 706
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 723
CseI GACGC 1 cut(s) 710
Csp6I GTAC 1 cut(s) 575
CviAII CATG 5 cut(s) 288, 311, 520, 532, 578
CviQI GTAC 1 cut(s) 575
DdeI CTNAG 3 cut(s) 206, 321, 339
DpnI GATC 3 cut(s) 34, 722, 726
DpnII GATC 3 cut(s) 32, 720, 724
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 519, 585
Eco24I GRGCYC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 325
EcoICRI GAGCTC 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 2 cut(s) 519, 585
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 519, 585
FaeI CATG 5 cut(s) 291, 314, 523, 535, 581
FatI CATG 5 cut(s) 287, 310, 519, 531, 577
FauNDI CATATG 1 cut(s) 8
Fnu4HI GCNGC 5 cut(s) 13, 65, 141, 635, 715
FokI GGATG 2 cut(s) 378, 668
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 5 cut(s) 13, 65, 141, 635, 715
FspAI RTGCGCAY 1 cut(s) 356
FspBI CTAG 3 cut(s) 36, 705, 711
FspI TGCGCA 1 cut(s) 356
GlaI GCGC 1 cut(s) 356
GluI GCNGC 5 cut(s) 13, 65, 141, 635, 715
HaeIII GGCC 1 cut(s) 99
HgaI GACGC 1 cut(s) 710
HhaI GCGC 1 cut(s) 357
Hin1II CATG 5 cut(s) 291, 314, 523, 535, 581
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HindIII AAGCTT 2 cut(s) 452, 484
HinfI GANTC 2 cut(s) 159, 528
HphI GGTGA 2 cut(s) 482, 574
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 1 cut(s) 399
Hpy188III TCNNGA 4 cut(s) 30, 36, 92, 182
Hpy8I GTNNAC 1 cut(s) 439
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 6 cut(s) 6, 296, 310, 458, 505, 717
HpyF10VI GCNNNNNNNGC 3 cut(s) 12, 293, 714
HpyF3I CTNAG 3 cut(s) 206, 321, 339
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 5 cut(s) 291, 314, 523, 535, 581
HspAI GCGC 1 cut(s) 355
Kzo9I GATC 3 cut(s) 32, 720, 724
LmnI GCTCC 3 cut(s) 214, 277, 330
LpnPI CCDG 7 cut(s) 30, 105, 281, 290, 314, 341, 615
Lsp1109I GCAGC 4 cut(s) 51, 127, 646, 701
LweI GCATC 3 cut(s) 182, 356, 503
MaeI CTAG 3 cut(s) 36, 705, 711
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 3 cut(s) 53, 316, 580
MalI GATC 3 cut(s) 34, 722, 726
MboI GATC 3 cut(s) 32, 720, 724
MboII GAAGA 2 cut(s) 197, 629
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 2 cut(s) 219, 327
MluCI AATT 5 cut(s) 117, 224, 382, 539, 646
MnlI CCTC 4 cut(s) 18, 111, 238, 623
MroXI GAANNNNTTC 2 cut(s) 78, 663
MseI TTAA 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 12, 293, 714
NcoI CCATGG 1 cut(s) 519
NdeI CATATG 1 cut(s) 8
NdeII GATC 3 cut(s) 32, 720, 724
NheI GCTAGC 1 cut(s) 704
NlaIII CATG 5 cut(s) 291, 314, 523, 535, 581
NmuCI GTSAC 2 cut(s) 316, 580
NsbI TGCGCA 1 cut(s) 356
NspI RCATGY 3 cut(s) 291, 314, 581
NspV TTCGAA 2 cut(s) 157, 667
PciI ACATGT 1 cut(s) 577
PdmI GAANNNNTTC 2 cut(s) 78, 663
PfeI GAWTC 2 cut(s) 159, 528
PfoI TCCNGGA 1 cut(s) 327
PkrI GCNGC 5 cut(s) 14, 66, 142, 636, 716
PscI ACATGT 1 cut(s) 577
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 327
PspGI CCWGG 1 cut(s) 327
PspPI GGNCC 1 cut(s) 98
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
SacI GAGCTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 5 cut(s) 13, 65, 141, 635, 715
Sau3AI GATC 3 cut(s) 32, 720, 724
Sau96I GGNCC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 2 cut(s) 219, 327
SetI ASST 9 cut(s) 29, 66, 207, 327, 456, 463, 488, 587, 716
SfaNI GCATC 3 cut(s) 182, 356, 503
SfcI CTRYAG 1 cut(s) 391
SfuI TTCGAA 2 cut(s) 157, 667
SmlI CTYRAG 1 cut(s) 487
SmoI CTYRAG 1 cut(s) 487
Sse9I AATT 5 cut(s) 117, 224, 382, 539, 646
SspI AATATT 1 cut(s) 235
SspMI CTAG 3 cut(s) 36, 705, 711
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 2 cut(s) 519, 585
TaiI ACGT 1 cut(s) 463
TaqI TCGA 3 cut(s) 157, 667, 723
TasI AATT 5 cut(s) 117, 224, 382, 539, 646
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 2 cut(s) 159, 528
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 2 cut(s) 316, 580
TseI GCWGC 5 cut(s) 12, 64, 140, 634, 714
Tsp45I GTSAC 2 cut(s) 316, 580
TspDTI ATGAA 8 cut(s) 123, 217, 376, 467, 520, 534, 630, 672
TspRI CASTG 1 cut(s) 325
XagI CCTNNNNNAGG 1 cut(s) 272
XapI RAATTY 3 cut(s) 224, 382, 539
XbaI TCTAGA 1 cut(s) 35
XceI RCATGY 3 cut(s) 291, 314, 581
XcmI CCANNNNNNNNNTGG 2 cut(s) 430, 645
XmnI GAANNNNTTC 2 cut(s) 78, 663
XspI CTAG 3 cut(s) 36, 705, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.