Rh3DG082500

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005149
Physical Location & Seq
Reverse (-)
6330347 .. 6338416
8070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG082500.1

Sequence Viewer

Length: 2703 bp
ATGGGTATCATATCCAGGAAGATTTTCCCAGCATGTGGGAACATGTGTGTATGCTGCCCTGCTATGAGGTCAAGATCTAGACAACCTGTTAAGCGTTACAAAAAACTGCTTGCAGAAATCTTTCCCAAGTCTCCTGATGGCCCTCCCAATGAAAGGAAAATTGTTAAGTTATGCGAATATGCTGCAAAGAATCCTTTTAGAATCCCTAAGATTGCCAAACATCTTGAAGATAGGTGTTACAAAGAGCTACGACTTGAGCATGTCAAATTCATCAATATTGTTGCAGAGGCCTACAAAAAGTTACTTTGCCTCTGTAAGGACCAGATGGCTTATTTTGCTGTCAGTGTGCTAAACGTAGTCACTGAGCTTCTGGACAACTCTAAGCAAGACGCTCTCCGTATAATTGGATGCCAAACTCTGACGAGGTTCATCTACACTCAGGCAGATGGAACTTATACACACAACATTGAGAGTTTGGTTCACAAAGTATGTAAGTTGGCACATGAGAGTGGTGAAGACCATCAAAAGCGCTGCTTGAGAGCATCAAGTTTGCAGTGCCTTTCAGCCATGGTCCAATTCATGACTGAATTTTCATATATTTTTGTTGATTTTGATGAGATTGTACATGCCACTTTGGATAACTATGAGCCTGATGCAAATGATGATGATGATGAGGGAGCGGAGTCGCATCATAATTGGGTGGATGAAGTAGTTCGAAGTGAAGGTAGAGTTGGTGCAATTGCCGGTGGGTATTCTAGCCCTAGTTGTAAGGTCATCAGGCCACGGCCAGAAAAAAAGGATCCTTCTCTTCTCACAAGAGAAGAGACAGAGACGCCAAACATTTGGGCTCAAATTTGTATCCAGAGAATGATTGAATTAGCCAAGGAGAGCACAACAATGCGCCGGGTATTGGATCCAATGTTTGTCTACTTTGACTCCAGGCACCATTGGGTTCCTCAACAAGGGTTGGCTTTGTCAGTTTTGTCTGATATGTCATACTTTTTGGAGACTTCAGGAAATCAGCAGATGATTTTAGCCTATGCAATCCGTCATCTTGACCACAAAAACGTTTCATATGATCCACAACTCAAATCTCAGATCATTCAAGTTGCTAGTGCTTTGGCAAGCCAAATTCGATCCGGAACCGTGCTAGAAGAAATTGGTTTTGTCTCTGACCTATGCAGGCATTTGAGGAAGAGTCTTCAAGCTACTGTTGAATCAGTTGGAGAGCAAGAGTCTAACATTAATACCATGCTACAGAGTTCCATTGAAGATTGCTTATTAGAAATTGCTAGAGGGATTGGAAATATAAGGCCATTGTTTGACATGATGTCTATATCTCTGGAGAAGCTTCCATCTGGAACTGTTGCCAGGGCGACCATGGGATCATTGATGATTGTTGCTCACATGATCTCATTAGCATTGATCTCTTCACATTCACAGCAGGTGTTTCCTGAATCCCTTCTTGTCCAGCTTTTGAAAGTGATGATGCATCCAGATGTTGAGGTGCGTGTTGGAGCACACCAGATATTTTCTGTTCTTCTCATTCCGTGTTCTAATCGGCCCCGACATGAAGTTGCTCCTCTACAATCTGGTTATGTATACCAGTCAAGAAAAGGGAATTCAAATACAGCATCTATTGCTTCAATTACAGCTAGACTTGAAAAGCTTAGGAGGGAAAAAGATGGCCCCAAAGCAGAAAATCAGGAGACTGATGCTCGTGATGATTTTAAAGATAGAGATACTGCAGAAGAAGATTGCAAGCAAGGGCATACCCACAAGAGTTCCCCGAACTTCTATAAAATTAGCTCTATTATTGACAAGACTGCTGGGTCAAGCCTAACAGAGCCTGAACCGTACGTAATGAAGTTTAGTGAGGATCAAATAGCAGACTTGCTGTCTGCTTTTTGGATACAAGCCAATCTCCCAGATAATTTGCCTTCAAATATTGAAGCTATAGCTCATTCTTTCATTTTAATGATAGTTTCTTCTCATTTGAAGAATCCAAATGGCAACCTTATGGTTCGTTCCTTTCAACTTCTGCTATCTCTGAGGAATATCTCCCTTGATCCTAACAATGGGATGTTGCCTCCAGCTTGCCAGAGATCTATCCTTGTGCTATCGATTGGCCTGTTGATGTTTTCTGCTCAGATCTATCACATTCCTAATCTAAATAATCTGCTCAAATCATTAATACCATCTGATGTTGATCCATATTTGGGAATCAGTGATGACCTTCAAGTTTTTCTAAGGCCTGAGGCAGACATCACAAAATATGGATCTGTTATCGATAACCAGCTAGCTTCATCATTACTATTTGATTTGCTGAAAAAATTATGTGAATCTGACGATGTCATAAGGGAAATCTTAGTTCAATTTCTATCAGACATTACTGAGATGGAGGCAGAGGCTGTGGCTAACCAACTTTTGGAGTCATTCGTACCTGATGATGCGTTCATGTTTGGTCCACAATCAATGCTTGACTTTGATGAAAATCAAATGCCAGGCCATTCGAAAGAATCACTTTCCTTTGATGGGGAATTTCCAACAAATTCATCAGTTGAGGATGATGCAACAAGTGAAGCATCAGTTGTTGCTGATTTCTCCCGGTTCATTCCTAGAGTCCCTTCATCCTCTTCTGTCTCCCATGTTATCAGTGTTGGACAGCTTCTGGAATCAGTAACCACTTCCTCCCTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

900

Amino Acids

100.77

Weight (kDa)

5.51

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 159 3.1e-21 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1435
AasI GACNNNNNNGTC 1 cut(s) 1831
Acc36I ACCTGC 1 cut(s) 1435
AccB1I GGYRCC 1 cut(s) 942
AccB7I CCANNNNNTGG 2 cut(s) 35, 2428
AccBSI CCGCTC 1 cut(s) 680
AccI GTMKAC 2 cut(s) 927, 1602
AccIII TCCGGA 1 cut(s) 1139
AciI CCGC 1 cut(s) 680
AclI AACGTT 1 cut(s) 1068
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 7 cut(s) 266, 587, 852, 1131, 1621, 2540, 2551
AcuI CTGAAG 1 cut(s) 996
AcyI GRCGYC 1 cut(s) 833
AfaI GTAC 3 cut(s) 624, 1859, 2442
AfeI AGCGCT 1 cut(s) 530
AfiI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2078, 2219, 2428, 2535
AflIII ACRYGT 1 cut(s) 42
AhdI GACNNNNNGTC 2 cut(s) 1330, 1897
AjnI CCWGG 4 cut(s) 14, 938, 1370, 2503
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
Alw21I GWGCWC 2 cut(s) 893, 1522
Alw26I GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 1703, 2647
AlwNI CAGNNNCTG 3 cut(s) 1850, 2411, 2671
Aor13HI TCCGGA 1 cut(s) 1139
Aor51HI AGCGCT 1 cut(s) 530
ApeKI GCWGC 3 cut(s) 54, 182, 531
ApoI RAATTY 7 cut(s) 266, 587, 852, 1131, 1621, 2540, 2551
AseI ATTAAT 2 cut(s) 1245, 2192
Asp700I GAANNNNTTC 4 cut(s) 23, 120, 711, 1461
AspLEI GCGC 2 cut(s) 531, 903
AspS9I GGNCC 6 cut(s) 140, 319, 571, 1563, 1688, 2465
AsuC2I CCSGG 2 cut(s) 905, 2608
AsuHPI GGTGA 1 cut(s) 524
AsuII TTCGAA 2 cut(s) 715, 2513
AsuNHI GCTAGC 1 cut(s) 2299
AvaII GGWCC 3 cut(s) 319, 571, 2465
AxyI CCTNAGG 1 cut(s) 2256
BamHI GGATCC 2 cut(s) 799, 913
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 1 cut(s) 850
BauI CACGAG 1 cut(s) 1719
BbsI GAAGAC 2 cut(s) 522, 1193
Bbv12I GWGCWC 2 cut(s) 893, 1522
BbvI GCAGC 3 cut(s) 41, 169, 518
BccI CCATC 9 cut(s) 131, 319, 440, 528, 1363, 1679, 2206, 2392, 2528
BceAI ACGGC 1 cut(s) 800
BcgI CGANNNNNNTGC 2 cut(s) 164, 198
BciT130I CCWGG 4 cut(s) 16, 940, 1372, 2505
BciVI GTATCC 2 cut(s) 869, 1905
BcnI CCSGG 2 cut(s) 905, 2608
BcoDI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 1703, 2647
BfaI CTAG 9 cut(s) 78, 756, 762, 1113, 1151, 1293, 1656, 2300, 2619
BfmI CTRYAG 3 cut(s) 1256, 1746, 1956
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 1 cut(s) 1435
BfuI GTATCC 2 cut(s) 869, 1905
BglII AGATCT 3 cut(s) 74, 2105, 2151
BisI GCNGC 3 cut(s) 55, 183, 532
BlsI GCNGC 3 cut(s) 56, 184, 533
Bme1390I CCNGG 6 cut(s) 16, 905, 940, 1372, 2505, 2608
Bme18I GGWCC 3 cut(s) 319, 571, 2465
BmeRI GACNNNNNGTC 2 cut(s) 1330, 1897
BmgT120I GGNCC 6 cut(s) 140, 319, 571, 1563, 1688, 2465
BmiI GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1565, 1690
BmrFI CCNGG 6 cut(s) 16, 905, 940, 1372, 2505, 2608
BmtI GCTAGC 1 cut(s) 2303
BpiI GAAGAC 2 cut(s) 522, 1193
BpmI CTGGAG 3 cut(s) 922, 1364, 2076
Bpu10I CCTNAGC 1 cut(s) 1670
Bpu14I TTCGAA 2 cut(s) 715, 2513
BpuEI CTTGAG 2 cut(s) 275, 556
BpuMI CCSGG 2 cut(s) 905, 2608
Bsa29I ATCGAT 2 cut(s) 2123, 2289
BsaAI YACGTR 1 cut(s) 1861
BsaBI GATNNNNATC 2 cut(s) 2208, 2493
BsaHI GRCGYC 1 cut(s) 833
BsaJI CCNNGG 5 cut(s) 567, 782, 882, 1371, 1380
BsaWI WCCGGW 1 cut(s) 1139
BsaXI ACNNNNNCTCC 2 cut(s) 920, 950
Bsc4I CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2078, 2219, 2428, 2535
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 1 cut(s) 1606
Bse21I CCTNAGG 1 cut(s) 2256
Bse8I GATNNNNATC 2 cut(s) 2208, 2493
BseAI TCCGGA 1 cut(s) 1139
BseBI CCWGG 4 cut(s) 16, 940, 1372, 2505
BseCI ATCGAT 2 cut(s) 2123, 2289
BseDI CCNNGG 5 cut(s) 567, 782, 882, 1371, 1380
BseGI GGATG 6 cut(s) 413, 709, 1492, 2088, 2572, 2630
BseJI GATNNNNATC 2 cut(s) 2208, 2493
BseLI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2078, 2219, 2428, 2535
BseMII CTCAG 7 cut(s) 354, 452, 1109, 2042, 2162, 2247, 2385
BseNI ACTGG 1 cut(s) 1606
BseRI GAGGAG 1 cut(s) 1572
BseXI GCAGC 3 cut(s) 41, 169, 518
BseYI CCCAGC 2 cut(s) 28, 1829
BshNI GGYRCC 1 cut(s) 942
BshVI ATCGAT 2 cut(s) 2123, 2289
BsiHKAI GWGCWC 2 cut(s) 893, 1522
BsiSI CCGG 4 cut(s) 744, 904, 1140, 2608
BsiWI CGTACG 1 cut(s) 1857
BslFI GGGAC 1 cut(s) 2609
BslI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2078, 2219, 2428, 2535
BsmAI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 1703, 2647
BsmBI CGTCTC 1 cut(s) 824
BsmFI GGGAC 1 cut(s) 2609
Bsp119I TTCGAA 2 cut(s) 715, 2513
Bsp1286I GDGCHC 3 cut(s) 850, 893, 1522
Bsp13I TCCGGA 1 cut(s) 1139
Bsp1407I TGTACA 1 cut(s) 622
Bsp19I CCATGG 2 cut(s) 567, 1380
BspACI CCGC 1 cut(s) 680
BspCNI CTCAG 7 cut(s) 355, 451, 1108, 2043, 2161, 2248, 2386
BspDI ATCGAT 2 cut(s) 2123, 2289
BspEI TCCGGA 1 cut(s) 1139
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1565, 1690
BspMAI CTGCAG 1 cut(s) 1750
BspMI ACCTGC 1 cut(s) 1435
BspOI GCTAGC 1 cut(s) 2303
BspT104I TTCGAA 2 cut(s) 715, 2513
BspT107I GGYRCC 1 cut(s) 942
BsrBI CCGCTC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 1 cut(s) 1606
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 5 cut(s) 567, 782, 882, 1371, 1380
BssNAI GTATAC 1 cut(s) 1603
BssNI GRCGYC 1 cut(s) 833
BssSI CACGAG 1 cut(s) 1719
BssT1I CCWWGG 3 cut(s) 567, 882, 1380
Bst1107I GTATAC 1 cut(s) 1603
Bst2BI CACGAG 1 cut(s) 1719
Bst2UI CCWGG 4 cut(s) 16, 940, 1372, 2505
Bst4CI ACNGT 4 cut(s) 1147, 1213, 1366, 1857
Bst6I CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2641
BstACI GRCGYC 1 cut(s) 833
BstAPI GCANNNNNTGC 1 cut(s) 1640
BstAUI TGTACA 1 cut(s) 622
BstBAI YACGTR 1 cut(s) 1861
BstBI TTCGAA 2 cut(s) 715, 2513
BstC8I GCNNGC 6 cut(s) 111, 1126, 1184, 1763, 2098, 2301
BstDSI CCRYGG 3 cut(s) 567, 782, 1380
BstENI CCTNNNNNAGG 2 cut(s) 314, 960
BstF5I GGATG 6 cut(s) 413, 709, 1492, 2088, 2572, 2630
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 2 cut(s) 531, 903
BstMAI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 1703, 2647
BstMWI GCNNNNNNNGC 2 cut(s) 335, 1640
BstNI CCWGG 4 cut(s) 16, 940, 1372, 2505
BstNSI RCATGY 4 cut(s) 36, 46, 263, 629
BstSCI CCNGG 6 cut(s) 14, 903, 938, 1370, 2503, 2606
BstSFI CTRYAG 3 cut(s) 1256, 1746, 1956
BstSNI TACGTA 1 cut(s) 1861
BstV1I GCAGC 3 cut(s) 41, 169, 518
BstV2I GAAGAC 2 cut(s) 522, 1193
BstX2I RGATCY 6 cut(s) 74, 799, 913, 2105, 2151, 2279
BstXI CCANNNNNNTGG 1 cut(s) 843
BstYI RGATCY 6 cut(s) 74, 799, 913, 2105, 2151, 2279
BstZ17I GTATAC 1 cut(s) 1603
Bsu15I ATCGAT 2 cut(s) 2123, 2289
Bsu36I CCTNAGG 1 cut(s) 2256
BsuI GTATCC 2 cut(s) 869, 1905
BsuTUI ATCGAT 2 cut(s) 2123, 2289
BtgI CCRYGG 3 cut(s) 567, 782, 1380
BtsCI GGATG 6 cut(s) 413, 709, 1492, 2088, 2572, 2630
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 5 cut(s) 349, 360, 560, 2233, 2662
BveI ACCTGC 1 cut(s) 1435
Cac8I GCNNGC 6 cut(s) 111, 1126, 1184, 1763, 2098, 2301
CaiI CAGNNNCTG 3 cut(s) 1850, 2411, 2671
CciI TCATGA 1 cut(s) 579
CfoI GCGC 2 cut(s) 531, 903
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 6 cut(s) 140, 319, 571, 1563, 1688, 2465
ClaI ATCGAT 2 cut(s) 2123, 2289
CseI GACGC 2 cut(s) 398, 841
Csp6I GTAC 3 cut(s) 623, 1858, 2441
CviQI GTAC 3 cut(s) 623, 1858, 2441
DraI TTTAAA 1 cut(s) 1732
DrdI GACNNNNNNGTC 1 cut(s) 1831
DriI GACNNNNNGTC 2 cut(s) 1330, 1897
DseDI GACNNNNNNGTC 1 cut(s) 1831
EaeI YGGCCR 1 cut(s) 785
Eam1104I CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2641
Eam1105I GACNNNNNGTC 2 cut(s) 1330, 1897
EarI CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2641
Eco105I TACGTA 1 cut(s) 1861
Eco130I CCWWGG 3 cut(s) 567, 882, 1380
Eco147I AGGCCT 2 cut(s) 290, 2254
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 3 cut(s) 319, 571, 2465
Eco47III AGCGCT 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 996
Eco81I CCTNAGG 1 cut(s) 2256
EcoNI CCTNNNNNAGG 2 cut(s) 314, 960
EcoRI GAATTC 1 cut(s) 1621
EcoRII CCWGG 4 cut(s) 14, 938, 1370, 2503
EcoT14I CCWWGG 3 cut(s) 567, 882, 1380
EcoT22I ATGCAT 1 cut(s) 1494
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 3 cut(s) 567, 882, 1380
Esp3I CGTCTC 1 cut(s) 824
FalI AAGNNNNNCTT 4 cut(s) 518, 550, 2508, 2540
FaqI GGGAC 1 cut(s) 2609
FauNDI CATATG 1 cut(s) 1075
FblI GTMKAC 2 cut(s) 927, 1602
Fnu4HI GCNGC 3 cut(s) 55, 183, 532
FokI GGATG 6 cut(s) 420, 716, 1479, 2095, 2579, 2617
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 3 cut(s) 55, 183, 532
FspBI CTAG 9 cut(s) 78, 756, 762, 1113, 1151, 1293, 1656, 2300, 2619
GlaI GCGC 2 cut(s) 530, 902
GluI GCNGC 3 cut(s) 55, 183, 532
GsaI CCCAGC 2 cut(s) 32, 1833
GsuI CTGGAG 3 cut(s) 922, 1364, 2076
HaeII RGCGCY 1 cut(s) 532
HapII CCGG 4 cut(s) 744, 904, 1140, 2608
HgaI GACGC 2 cut(s) 398, 841
HhaI GCGC 2 cut(s) 531, 903
Hin1I GRCGYC 1 cut(s) 833
Hin6I GCGC 2 cut(s) 529, 901
HinP1I GCGC 2 cut(s) 529, 901
HindIII AAGCTT 2 cut(s) 1349, 1667
HpaII CCGG 4 cut(s) 744, 904, 1140, 2608
HphI GGTGA 1 cut(s) 524
Hpy166II GTNNAC 4 cut(s) 481, 928, 1603, 2468
Hpy8I GTNNAC 4 cut(s) 481, 928, 1603, 2468
HpyAV CCTTC 6 cut(s) 716, 813, 1472, 1950, 2246, 2637
HpyCH4III ACNGT 4 cut(s) 1147, 1213, 1366, 1857
HpyCH4IV ACGT 3 cut(s) 354, 1068, 1860
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 1640
HpySE526I ACGT 3 cut(s) 354, 1068, 1860
Hsp92I GRCGYC 1 cut(s) 833
HspAI GCGC 2 cut(s) 529, 901
Kpn2I TCCGGA 1 cut(s) 1139
LmnI GCTCC 3 cut(s) 677, 1517, 1585
Lsp1109I GCAGC 3 cut(s) 41, 169, 518
MaeI CTAG 9 cut(s) 78, 756, 762, 1113, 1151, 1293, 1656, 2300, 2619
MaeII ACGT 3 cut(s) 354, 1068, 1860
MaeIII GTNAC 5 cut(s) 95, 236, 300, 358, 2680
MbiI CCGCTC 1 cut(s) 680
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 6 cut(s) 74, 799, 913, 2105, 2151, 2279
MhlI GDGCHC 3 cut(s) 850, 893, 1522
MlyI GAGTC 6 cut(s) 692, 929, 1207, 1244, 2441, 2631
MmeI TCCRAC 4 cut(s) 1204, 1495, 2570, 2641
Mph1103I ATGCAT 1 cut(s) 1494
MroI TCCGGA 1 cut(s) 1139
MroXI GAANNNNTTC 4 cut(s) 23, 120, 711, 1461
MseI TTAA 6 cut(s) 90, 165, 1245, 1731, 1976, 2192
MslI CAYNNNNRTG 4 cut(s) 507, 896, 1497, 1976
MspI CCGG 4 cut(s) 744, 904, 1140, 2608
MspR9I CCNGG 6 cut(s) 16, 905, 940, 1372, 2505, 2608
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 4 cut(s) 16, 940, 1372, 2505
MwoI GCNNNNNNNGC 2 cut(s) 335, 1640
NciI CCSGG 2 cut(s) 905, 2608
NcoI CCATGG 2 cut(s) 567, 1380
NdeI CATATG 1 cut(s) 1075
NheI GCTAGC 1 cut(s) 2299
NlaIV GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1565, 1690
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 1494
NspI RCATGY 4 cut(s) 36, 46, 263, 629
NspV TTCGAA 2 cut(s) 715, 2513
PagI TCATGA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 1435
PceI AGGCCT 2 cut(s) 290, 2254
PciI ACATGT 1 cut(s) 42
PdmI GAANNNNTTC 4 cut(s) 23, 120, 711, 1461
PfeI GAWTC 9 cut(s) 190, 201, 1217, 1457, 2002, 2223, 2342, 2519, 2675
Pfl23II CGTACG 1 cut(s) 1857
PflFI GACNNNGTC 1 cut(s) 2351
PflMI CCANNNNNTGG 2 cut(s) 35, 2428
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 3 cut(s) 56, 184, 533
PleI GAGTC 6 cut(s) 691, 929, 1206, 1243, 2440, 2630
PpsI GAGTC 6 cut(s) 691, 929, 1206, 1243, 2440, 2630
Ppu21I YACGTR 1 cut(s) 1861
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 2 cut(s) 1245, 2192
Psp1406I AACGTT 1 cut(s) 1068
Psp6I CCWGG 4 cut(s) 14, 938, 1370, 2503
PspFI CCCAGC 2 cut(s) 28, 1829
PspGI CCWGG 4 cut(s) 14, 938, 1370, 2503
PspLI CGTACG 1 cut(s) 1857
PspN4I GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1565, 1690
PspPI GGNCC 6 cut(s) 140, 319, 571, 1563, 1688, 2465
PstI CTGCAG 1 cut(s) 1750
PstNI CAGNNNCTG 3 cut(s) 1850, 2411, 2671
PsuI RGATCY 6 cut(s) 74, 799, 913, 2105, 2151, 2279
PsyI GACNNNGTC 1 cut(s) 2351
RsaI GTAC 3 cut(s) 624, 1859, 2442
RsaNI GTAC 3 cut(s) 623, 1858, 2441
RseI CAYNNNNRTG 4 cut(s) 507, 896, 1497, 1976
SaqAI TTAA 6 cut(s) 90, 165, 1245, 1731, 1976, 2192
SatI GCNGC 3 cut(s) 55, 183, 532
Sau96I GGNCC 6 cut(s) 140, 319, 571, 1563, 1688, 2465
SchI GAGTC 6 cut(s) 692, 929, 1207, 1244, 2441, 2631
ScrFI CCNGG 6 cut(s) 16, 905, 940, 1372, 2505, 2608
SduI GDGCHC 3 cut(s) 850, 893, 1522
SfcI CTRYAG 3 cut(s) 1256, 1746, 1956
SfuI TTCGAA 2 cut(s) 715, 2513
SinI GGWCC 3 cut(s) 319, 571, 2465
SmiMI CAYNNNNRTG 4 cut(s) 507, 896, 1497, 1976
SmlI CTYRAG 2 cut(s) 254, 535
SmoI CTYRAG 2 cut(s) 254, 535
SnaBI TACGTA 1 cut(s) 1861
SseBI AGGCCT 2 cut(s) 290, 2254
SsiI CCGC 1 cut(s) 680
SspI AATATT 2 cut(s) 277, 1948
SspMI CTAG 9 cut(s) 78, 756, 762, 1113, 1151, 1293, 1656, 2300, 2619
StuI AGGCCT 2 cut(s) 290, 2254
StyD4I CCNGG 6 cut(s) 14, 903, 938, 1370, 2503, 2606
StyI CCWWGG 3 cut(s) 567, 882, 1380
TaaI ACNGT 4 cut(s) 1147, 1213, 1366, 1857
TaiI ACGT 3 cut(s) 357, 1071, 1863
TaqI TCGA 5 cut(s) 715, 1135, 2123, 2289, 2513
TatI WGTACW 1 cut(s) 622
TfiI GAWTC 9 cut(s) 190, 201, 1217, 1457, 2002, 2223, 2342, 2519, 2675
Tru1I TTAA 6 cut(s) 90, 165, 1245, 1731, 1976, 2192
Tru9I TTAA 6 cut(s) 90, 165, 1245, 1731, 1976, 2192
TscAI CASTG 5 cut(s) 349, 367, 560, 2233, 2662
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 3 cut(s) 54, 182, 531
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 3 cut(s) 386, 1037, 1539
TspRI CASTG 5 cut(s) 349, 367, 560, 2233, 2662
Tth111I GACNNNGTC 1 cut(s) 2351
Van91I CCANNNNNTGG 2 cut(s) 35, 2428
VpaK11BI GGWCC 3 cut(s) 319, 571, 2465
VspI ATTAAT 2 cut(s) 1245, 2192
XagI CCTNNNNNAGG 2 cut(s) 314, 960
XapI RAATTY 7 cut(s) 266, 587, 852, 1131, 1621, 2540, 2551
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 4 cut(s) 36, 46, 263, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 1378
XmiI GTMKAC 2 cut(s) 927, 1602
XmnI GAANNNNTTC 4 cut(s) 23, 120, 711, 1461
XspI CTAG 9 cut(s) 78, 756, 762, 1113, 1151, 1293, 1656, 2300, 2619
Zsp2I ATGCAT 1 cut(s) 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.