RchiOBHm_Chr3g0457131

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005149
Physical Location & Seq
Reverse (-)
6351001 .. 6359675
8675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42389

Sequence Viewer

Length: 3012 bp
ATGGGTATCATATCCAGGAAGATTTTCCCAGCATGTGGGAACATGTGTGTGTGCTGCCCTGCTATGAGGTCAAGATCTAGACAACCTGTTAAGCGTTACAAAAAACTGCTTGCAGAAATCTTTCCCAAGTCTCCTGATGGCCCTCCCAATGAAAGGAAAATTGTTAAGTTATGCGAATATGCTGCAAAGAATCCTTTTAGAATCCCCAAGATTGCCAAACATCTTGAAGATAGGTGTTACAAAGAGCTGCGACTTGAGCATGTCAAATTCATCAATATTGTTGCAGAGGCCTACAACAAGTTACTTTGCCTCTGTAAGGACCAGATGGCTTATTTTGCTGTCAGTGTGCTAAACGTAGTCACTGAGCTTCTGGACAACTCTAAGCAAGACGCTCTCCGTATAATTGGATGCCAAACTCTGACGAGGTTCATCTACACTCAGGCAGATGGAACTTATACACACAACATTGAGAGTTTGGTTCACAAAGTATGTAAGTTGGCACATGAGAGTGGTGAAGACCATCAAAAGCGCTGCTTGAGAGCATCAAGTTTGCAGTGCCTTTCAGCCATGGTCCAATTCATGACTGAATTTTCATATATTTTTGTTGATTTTGATGAGATTGTACATGCCACTTTGGATAACTATGAGCCTGATGCAAATGATGATGATGATGAGGGAGCGGAGTCGCATCATAATTGGGTGGATGAAGTAGTTCGAAGTGAAGGTAGAGTTGGTGCAATTGCCGGTGGGTATTCTAGCCCTAGTTGTAAGGTCATCAGGCCACGGCCAGAAAAAAAGGATCCTTCTCTTCTCACAAGAGAAGAGACAGAGACGCCAAACATTTGGGCTCAAATTTGTATCCAGAGAATGATTGAATTAGCCAAGGAGAGCACAACAATGCGCCGGGTATTGGATCCAATGTTTGTCTACTTTGACTCTAGGCACCATTGGGTTCCTCGACAAGGGTTGGCTTTGTCAGTTTTGTCTGATATGTCATACTTTTTGGAGACTTCAGGAAATCAGCAGATGATTTTAGCCTATGCAATCCGTCATCTTGACCACAAAAACGTTTCACATGATCCACAACTCAAATCTCAGATCATTCAAGTTGCTAGTGCTTTGGCAAGCCAAATTCGATCCGGAACCGTGCTAGAAGAAATTGGTTTTGTCTCTGACCTATGCAGGCATTTGAGGAAGAGTCTTCAAGCTACTGTTGAATCAGTTGGAGAGCAAGAGTCTAACATTAATACCATGCTACAGAGTTCCATTGAAGATTGCTTATTAGAAATTGCTAGAGGGATTGGAAATATAAGACCATTGTTTGACATGATGTCTATATCTCTGGAGAAGCTTCCATCTGGAACTGTTGCCAGGGCGACCATGGGATCATTGATGATTGTTGCTCACATGATCTCATTAGCATTGATCTCTTCACATTCACAGCAGCAGGTGTTTCCTGAATCCCTTCTTGTCCAGCTTTTGAAAGTGATGATGCATCCAGATGTTGAGGTGCGTGTTGGAGCACACCAGATATTTTCTGTTCTTCTCATTCCATGTTCTAATCGGCCCCGACATGAAGTTGCTCCTCTACAATCTGGTTATGTATACCAGTCAAGAAAAGGGAATTCAAATACAGCATCTATTGCTTCAATTACGGCTAGACTTGAAAAGCTTAGGAGGGAAAAAGATGGCCCCAAAGCAGAAAATCAGGAGATTGATGCTCGTGATGATTTTAAAGATAGAGATACTGCAGAAGAAGATTGCAAGCAAGGGCATACCCACAAGAGTTCCCCGAACTTCTATAAAATTAGCTCTATTATTGACAAGACTGCTGGGTCAAGCCTAACAGAGCCTGAACCGTACGTAATGAAGTTTAGTGAGGATCAAATAGCAGACTTGTTGTCTGCTTTTTGGATACAAGCCAATCTCCCAGATAATTTACCTTCAAATATTGAAGCTATAGCTCATTCTTTCATTTTAATGATAGTTTCTTCTCATTTGAAGAATCCAAATGGCAACCTTATGGTTCGTTCCTTTCAACTTCTGCTATCTCTGAGGAATATCTCCCTTGATCCTAACAATGGGATGTTGCCTCCAGCTTGCCAGAGATCTATCCTTGTGCTATCGATTGGCCTGTTGATGTTTTCTGCTCAGATCTATCACATTCCTAATCTAAATAATCTGCTCAAATCATTAATACCATCTGATGTTGATCCATATTTGGGAATCAGTGATGACCTTCAAGTTTTTCTAAGGCCTGAGGCAGACATCACAAAATATGGATCTGTTATTGATAACCAGCTAGCTTCATCAGTACTATTTGATTTGCGGAAAAAATTATATGAATCTGACGATCTCATAAGGGAAATCTTAGTTCAATTTCTATCAGACATTACTGAGATGGAGGCAGAGGCTGTGGCTAACCTACTTTTGGAGTCATTCGTACCTGATGATGCGTTCATGTTTGGTCCACAATCAATGCTTGACTTTGATGAAAATCAAATGCCAGGCCATTCGAAAGAATCACTTTCCTTTGATGGGGAATTTCCGACAAATTCATCAATTGAGGATGATGCAACAAGTGAAGCATCAGTTGTTGCTGATTTCTCCCGGTTCATTCCTAGAGTCCCTTCATCCTCTTCTGTCTCCCATGTTATCAGTGTTGGACAGCTTCTGGAATCAGCACTTGAGGTTGCTGGTCAAGTAGCAGGAACATCTGTATCTACATCACCCCTCCCGTACAACACCATGGCAAGACACTGTGAAGCCCTTGGCACAGGCACAAGGAAGAAGCTTTCTAATTGGTTGGCCCATGAACATCATCAAAGCAACACTGTCCGTGACATTTTGTCTCCACCATTTCTTGCAGGTGGATGCCCAGCACTCCAGAAGCTAATTAATGAACCAGGCATTACCCGGGGAGCTGCATCGGCACAGGACTCGTGGTTAGCTGCTATCAGGTTGCCTCCTGCTAGTCCGTTTGACAACTTCCTCAAGGCAGCTGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1003

Amino Acids

111.69

Weight (kDa)

5.77

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 159 1.9e-21 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1438, 2858
AasI GACNNNNNNGTC 1 cut(s) 1834
Acc36I ACCTGC 2 cut(s) 1438, 2858
AccB1I GGYRCC 1 cut(s) 942
AccB7I CCANNNNNTGG 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 680
AccI GTMKAC 2 cut(s) 927, 1605
AccIII TCCGGA 1 cut(s) 1139
AciI CCGC 2 cut(s) 680, 2329
AclI AACGTT 1 cut(s) 1068
AcoI YGGCCR 1 cut(s) 785
AcsI RAATTY 7 cut(s) 266, 587, 852, 1131, 1624, 2543, 2554
AcuI CTGAAG 1 cut(s) 996
AcyI GRCGYC 1 cut(s) 833
AfaI GTAC 5 cut(s) 624, 1862, 2316, 2445, 2741
AfeI AGCGCT 1 cut(s) 530
AfiI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2081, 2222, 2431, 2538
AflIII ACRYGT 1 cut(s) 42
AhdI GACNNNNNGTC 3 cut(s) 1330, 1900, 2848
AjnI CCWGG 4 cut(s) 14, 1370, 2506, 2905
AjuI GAANNNNNNNTTGG 2 cut(s) 714, 746
Alw21I GWGCWC 2 cut(s) 893, 1525
Alw26I GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 2650, 2856
AlwNI CAGNNNCTG 3 cut(s) 1853, 2414, 2674
Ama87I CYCGRG 1 cut(s) 2916
Aor13HI TCCGGA 1 cut(s) 1139
Aor51HI AGCGCT 1 cut(s) 530
ApeKI GCWGC 8 cut(s) 54, 182, 247, 531, 1444, 2924, 2951, 2999
ApoI RAATTY 7 cut(s) 266, 587, 852, 1131, 1624, 2543, 2554
AseI ATTAAT 3 cut(s) 1245, 2195, 2898
Asp700I GAANNNNTTC 4 cut(s) 23, 120, 711, 1464
AspLEI GCGC 2 cut(s) 531, 903
AspS9I GGNCC 7 cut(s) 140, 319, 571, 1566, 1691, 2468, 2809
AsuC2I CCSGG 4 cut(s) 905, 2611, 2917, 2918
AsuHPI GGTGA 2 cut(s) 524, 2721
AsuII TTCGAA 2 cut(s) 715, 2516
AsuNHI GCTAGC 1 cut(s) 2302
AvaI CYCGRG 1 cut(s) 2916
AvaII GGWCC 3 cut(s) 319, 571, 2468
AxyI CCTNAGG 1 cut(s) 2259
BaeI ACNNNNGTAYC 2 cut(s) 2703, 2736
BamHI GGATCC 2 cut(s) 799, 913
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 1 cut(s) 850
BauI CACGAG 2 cut(s) 1722, 2941
BbsI GAAGAC 2 cut(s) 522, 1193
Bbv12I GWGCWC 2 cut(s) 893, 1525
BbvI GCAGC 7 cut(s) 41, 169, 234, 518, 1456, 2911, 2938
BccI CCATC 9 cut(s) 131, 319, 440, 528, 1363, 1682, 2209, 2395, 2531
BceAI ACGGC 2 cut(s) 800, 1671
BcgI CGANNNNNNTGC 4 cut(s) 164, 198, 2922, 2956
BciT130I CCWGG 4 cut(s) 16, 1372, 2508, 2907
BciVI GTATCC 2 cut(s) 869, 1908
BcnI CCSGG 4 cut(s) 905, 2611, 2917, 2918
BcoDI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 2650, 2856
BfmI CTRYAG 3 cut(s) 1256, 1749, 1959
BfoI RGCGCY 1 cut(s) 532
BfuAI ACCTGC 2 cut(s) 1438, 2858
BfuI GTATCC 2 cut(s) 869, 1908
BglII AGATCT 3 cut(s) 74, 2108, 2154
BisI GCNGC 8 cut(s) 55, 183, 248, 532, 1445, 2925, 2952, 3000
BlsI GCNGC 8 cut(s) 56, 184, 249, 533, 1446, 2926, 2953, 3001
BmcAI AGTACT 1 cut(s) 2316
Bme1390I CCNGG 8 cut(s) 16, 905, 1372, 2508, 2611, 2907, 2917, 2918
Bme18I GGWCC 3 cut(s) 319, 571, 2468
BmeRI GACNNNNNGTC 3 cut(s) 1330, 1900, 2848
BmeT110I CYCGRG 1 cut(s) 2916
BmgT120I GGNCC 7 cut(s) 140, 319, 571, 1566, 1691, 2468, 2809
BmiI GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1568, 1693
BmrFI CCNGG 8 cut(s) 16, 905, 1372, 2508, 2611, 2907, 2917, 2918
BmtI GCTAGC 1 cut(s) 2306
BpiI GAAGAC 2 cut(s) 522, 1193
BpmI CTGGAG 3 cut(s) 1364, 2079, 2870
Bpu10I CCTNAGC 1 cut(s) 1673
Bpu14I TTCGAA 2 cut(s) 715, 2516
BpuEI CTTGAG 4 cut(s) 275, 556, 2708, 2978
BpuMI CCSGG 4 cut(s) 905, 2611, 2917, 2918
Bsa29I ATCGAT 1 cut(s) 2126
BsaAI YACGTR 1 cut(s) 1864
BsaBI GATNNNNATC 2 cut(s) 2211, 2496
BsaHI GRCGYC 1 cut(s) 833
BsaJI CCNNGG 9 cut(s) 567, 782, 882, 1371, 1380, 2748, 2770, 2916, 2917
BsaWI WCCGGW 1 cut(s) 1139
Bsc4I CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2081, 2222, 2431, 2538
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 1 cut(s) 1609
Bse21I CCTNAGG 1 cut(s) 2259
Bse8I GATNNNNATC 2 cut(s) 2211, 2496
BseAI TCCGGA 1 cut(s) 1139
BseBI CCWGG 4 cut(s) 16, 1372, 2508, 2907
BseCI ATCGAT 1 cut(s) 2126
BseDI CCNNGG 9 cut(s) 567, 782, 882, 1371, 1380, 2748, 2770, 2916, 2917
BseGI GGATG 7 cut(s) 413, 709, 1495, 2091, 2575, 2633, 2879
BseJI GATNNNNATC 2 cut(s) 2211, 2496
BseLI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2081, 2222, 2431, 2538
BseMII CTCAG 7 cut(s) 354, 452, 1109, 2045, 2165, 2250, 2388
BseNI ACTGG 1 cut(s) 1609
BseRI GAGGAG 1 cut(s) 1575
BseXI GCAGC 7 cut(s) 41, 169, 234, 518, 1456, 2911, 2938
BseYI CCCAGC 3 cut(s) 28, 1832, 2878
BshNI GGYRCC 1 cut(s) 942
BshVI ATCGAT 1 cut(s) 2126
BsiHKAI GWGCWC 2 cut(s) 893, 1525
BsiHKCI CYCGRG 1 cut(s) 2916
BsiSI CCGG 5 cut(s) 744, 904, 1140, 2611, 2917
BsiWI CGTACG 1 cut(s) 1860
BslFI GGGAC 1 cut(s) 2612
BslI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 910, 962, 2081, 2222, 2431, 2538
BsmAI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 2650, 2856
BsmBI CGTCTC 1 cut(s) 824
BsmFI GGGAC 1 cut(s) 2612
BsoBI CYCGRG 1 cut(s) 2916
Bsp119I TTCGAA 2 cut(s) 715, 2516
Bsp1286I GDGCHC 3 cut(s) 850, 893, 1525
Bsp13I TCCGGA 1 cut(s) 1139
Bsp1407I TGTACA 1 cut(s) 622
Bsp19I CCATGG 3 cut(s) 567, 1380, 2748
BspACI CCGC 2 cut(s) 680, 2329
BspCNI CTCAG 7 cut(s) 355, 451, 1108, 2046, 2164, 2251, 2389
BspDI ATCGAT 1 cut(s) 2126
BspEI TCCGGA 1 cut(s) 1139
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1568, 1693
BspMAI CTGCAG 1 cut(s) 1753
BspMI ACCTGC 2 cut(s) 1438, 2858
BspOI GCTAGC 1 cut(s) 2306
BspT104I TTCGAA 2 cut(s) 715, 2516
BspT107I GGYRCC 1 cut(s) 942
BsrBI CCGCTC 1 cut(s) 680
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 1 cut(s) 1609
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 9 cut(s) 567, 782, 882, 1371, 1380, 2748, 2770, 2916, 2917
BssNAI GTATAC 1 cut(s) 1606
BssNI GRCGYC 1 cut(s) 833
BssSI CACGAG 2 cut(s) 1722, 2941
BssT1I CCWWGG 5 cut(s) 567, 882, 1380, 2748, 2770
Bst1107I GTATAC 1 cut(s) 1606
Bst2BI CACGAG 2 cut(s) 1722, 2941
Bst2UI CCWGG 4 cut(s) 16, 1372, 2508, 2907
Bst4CI ACNGT 6 cut(s) 1147, 1213, 1366, 1860, 2762, 2836
Bst6I CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2644
BstACI GRCGYC 1 cut(s) 833
BstAPI GCANNNNNTGC 1 cut(s) 1643
BstAUI TGTACA 1 cut(s) 622
BstBAI YACGTR 1 cut(s) 1864
BstBI TTCGAA 2 cut(s) 715, 2516
BstC8I GCNNGC 6 cut(s) 111, 1126, 1184, 1766, 2101, 2304
BstDSI CCRYGG 4 cut(s) 567, 782, 1380, 2748
BstENI CCTNNNNNAGG 2 cut(s) 314, 960
BstF5I GGATG 7 cut(s) 413, 709, 1495, 2091, 2575, 2633, 2879
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 2 cut(s) 531, 903
BstMAI GTCTC 7 cut(s) 135, 818, 824, 1001, 1174, 2650, 2856
BstMWI GCNNNNNNNGC 4 cut(s) 256, 335, 1643, 2930
BstNI CCWGG 4 cut(s) 16, 1372, 2508, 2907
BstNSI RCATGY 4 cut(s) 36, 46, 263, 629
BstSCI CCNGG 8 cut(s) 14, 903, 1370, 2506, 2609, 2905, 2915, 2916
BstSFI CTRYAG 3 cut(s) 1256, 1749, 1959
BstSNI TACGTA 1 cut(s) 1864
BstV1I GCAGC 7 cut(s) 41, 169, 234, 518, 1456, 2911, 2938
BstV2I GAAGAC 2 cut(s) 522, 1193
BstX2I RGATCY 6 cut(s) 74, 799, 913, 2108, 2154, 2282
BstXI CCANNNNNNTGG 1 cut(s) 843
BstYI RGATCY 6 cut(s) 74, 799, 913, 2108, 2154, 2282
BstZ17I GTATAC 1 cut(s) 1606
Bsu15I ATCGAT 1 cut(s) 2126
Bsu36I CCTNAGG 1 cut(s) 2259
BsuI GTATCC 2 cut(s) 869, 1908
BsuTUI ATCGAT 1 cut(s) 2126
BtgI CCRYGG 4 cut(s) 567, 782, 1380, 2748
BtsCI GGATG 7 cut(s) 413, 709, 1495, 2091, 2575, 2633, 2879
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 7 cut(s) 349, 360, 560, 2236, 2665, 2758, 2832
BveI ACCTGC 2 cut(s) 1438, 2858
Cac8I GCNNGC 6 cut(s) 111, 1126, 1184, 1766, 2101, 2304
CaiI CAGNNNCTG 3 cut(s) 1853, 2414, 2674
CciI TCATGA 1 cut(s) 579
CfoI GCGC 2 cut(s) 531, 903
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 7 cut(s) 140, 319, 571, 1566, 1691, 2468, 2809
Cfr9I CCCGGG 1 cut(s) 2916
ClaI ATCGAT 1 cut(s) 2126
CseI GACGC 2 cut(s) 398, 841
Csp6I GTAC 5 cut(s) 623, 1861, 2315, 2444, 2740
CviQI GTAC 5 cut(s) 623, 1861, 2315, 2444, 2740
DraI TTTAAA 1 cut(s) 1735
DrdI GACNNNNNNGTC 1 cut(s) 1834
DriI GACNNNNNGTC 3 cut(s) 1330, 1900, 2848
DseDI GACNNNNNNGTC 1 cut(s) 1834
EaeI YGGCCR 1 cut(s) 785
Eam1104I CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2644
Eam1105I GACNNNNNGTC 3 cut(s) 1330, 1900, 2848
EarI CTCTTC 5 cut(s) 813, 816, 1190, 1435, 2644
Eco105I TACGTA 1 cut(s) 1864
Eco130I CCWWGG 5 cut(s) 567, 882, 1380, 2748, 2770
Eco147I AGGCCT 2 cut(s) 290, 2257
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 3 cut(s) 319, 571, 2468
Eco47III AGCGCT 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 996
Eco81I CCTNAGG 1 cut(s) 2259
Eco88I CYCGRG 1 cut(s) 2916
EcoNI CCTNNNNNAGG 2 cut(s) 314, 960
EcoRI GAATTC 1 cut(s) 1624
EcoRII CCWGG 4 cut(s) 14, 1370, 2506, 2905
EcoT14I CCWWGG 5 cut(s) 567, 882, 1380, 2748, 2770
EcoT22I ATGCAT 1 cut(s) 1497
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 5 cut(s) 567, 882, 1380, 2748, 2770
Esp3I CGTCTC 1 cut(s) 824
FalI AAGNNNNNCTT 4 cut(s) 518, 550, 2511, 2543
FaqI GGGAC 1 cut(s) 2612
FblI GTMKAC 2 cut(s) 927, 1605
Fnu4HI GCNGC 8 cut(s) 55, 183, 248, 532, 1445, 2925, 2952, 3000
FokI GGATG 7 cut(s) 420, 716, 1482, 2098, 2582, 2620, 2886
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 8 cut(s) 55, 183, 248, 532, 1445, 2925, 2952, 3000
GlaI GCGC 2 cut(s) 530, 902
GluI GCNGC 8 cut(s) 55, 183, 248, 532, 1445, 2925, 2952, 3000
GsaI CCCAGC 3 cut(s) 32, 1836, 2882
GsuI CTGGAG 3 cut(s) 1364, 2079, 2870
HaeII RGCGCY 1 cut(s) 532
HapII CCGG 5 cut(s) 744, 904, 1140, 2611, 2917
HgaI GACGC 2 cut(s) 398, 841
HhaI GCGC 2 cut(s) 531, 903
Hin1I GRCGYC 1 cut(s) 833
Hin6I GCGC 2 cut(s) 529, 901
HinP1I GCGC 2 cut(s) 529, 901
HindIII AAGCTT 3 cut(s) 1349, 1670, 2792
HpaII CCGG 5 cut(s) 744, 904, 1140, 2611, 2917
HphI GGTGA 2 cut(s) 524, 2721
Hpy166II GTNNAC 4 cut(s) 481, 928, 1606, 2471
Hpy8I GTNNAC 4 cut(s) 481, 928, 1606, 2471
HpyAV CCTTC 6 cut(s) 716, 813, 1475, 1953, 2249, 2640
HpyCH4III ACNGT 6 cut(s) 1147, 1213, 1366, 1860, 2762, 2836
HpyCH4IV ACGT 3 cut(s) 354, 1068, 1863
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 335, 1643, 2930
HpySE526I ACGT 3 cut(s) 354, 1068, 1863
Hsp92I GRCGYC 1 cut(s) 833
HspAI GCGC 2 cut(s) 529, 901
Kpn2I TCCGGA 1 cut(s) 1139
LmnI GCTCC 4 cut(s) 677, 1520, 1588, 2921
Lsp1109I GCAGC 7 cut(s) 41, 169, 234, 518, 1456, 2911, 2938
MaeII ACGT 3 cut(s) 354, 1068, 1863
MaeIII GTNAC 5 cut(s) 95, 236, 300, 358, 2840
MbiI CCGCTC 1 cut(s) 680
MfeI CAATTG 2 cut(s) 738, 2562
MflI RGATCY 6 cut(s) 74, 799, 913, 2108, 2154, 2282
MhlI GDGCHC 3 cut(s) 850, 893, 1525
MlyI GAGTC 7 cut(s) 692, 929, 1207, 1244, 2444, 2634, 2933
MmeI TCCRAC 4 cut(s) 1204, 1498, 2573, 2644
Mph1103I ATGCAT 1 cut(s) 1497
MroI TCCGGA 1 cut(s) 1139
MroXI GAANNNNTTC 4 cut(s) 23, 120, 711, 1464
MseI TTAA 7 cut(s) 90, 165, 1245, 1734, 1979, 2195, 2898
MslI CAYNNNNRTG 5 cut(s) 47, 507, 896, 1500, 1979
MspA1I CMGCKG 1 cut(s) 3002
MspI CCGG 5 cut(s) 744, 904, 1140, 2611, 2917
MspR9I CCNGG 8 cut(s) 16, 905, 1372, 2508, 2611, 2907, 2917, 2918
MunI CAATTG 2 cut(s) 738, 2562
MvaI CCWGG 4 cut(s) 16, 1372, 2508, 2907
MwoI GCNNNNNNNGC 4 cut(s) 256, 335, 1643, 2930
NciI CCSGG 4 cut(s) 905, 2611, 2917, 2918
NcoI CCATGG 3 cut(s) 567, 1380, 2748
NheI GCTAGC 1 cut(s) 2302
NlaIV GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1568, 1693
NmuCI GTSAC 2 cut(s) 358, 2840
NsiI ATGCAT 1 cut(s) 1497
NspI RCATGY 4 cut(s) 36, 46, 263, 629
NspV TTCGAA 2 cut(s) 715, 2516
PagI TCATGA 1 cut(s) 579
PaqCI CACCTGC 2 cut(s) 1438, 2858
PceI AGGCCT 2 cut(s) 290, 2257
PciI ACATGT 1 cut(s) 42
PdmI GAANNNNTTC 4 cut(s) 23, 120, 711, 1464
PfeI GAWTC 9 cut(s) 190, 201, 1217, 1460, 2005, 2226, 2345, 2522, 2678
Pfl23II CGTACG 1 cut(s) 1860
PflMI CCANNNNNTGG 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 8 cut(s) 56, 184, 249, 533, 1446, 2926, 2953, 3001
PleI GAGTC 7 cut(s) 691, 929, 1206, 1243, 2443, 2633, 2933
PpsI GAGTC 7 cut(s) 691, 929, 1206, 1243, 2443, 2633, 2933
Ppu21I YACGTR 1 cut(s) 1864
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 3 cut(s) 1245, 2195, 2898
Psp1406I AACGTT 1 cut(s) 1068
Psp6I CCWGG 4 cut(s) 14, 1370, 2506, 2905
PspFI CCCAGC 3 cut(s) 28, 1832, 2878
PspGI CCWGG 4 cut(s) 14, 1370, 2506, 2905
PspLI CGTACG 1 cut(s) 1860
PspN4I GGNNCC 7 cut(s) 801, 915, 944, 954, 1144, 1568, 1693
PspPI GGNCC 7 cut(s) 140, 319, 571, 1566, 1691, 2468, 2809
PstI CTGCAG 1 cut(s) 1753
PstNI CAGNNNCTG 3 cut(s) 1853, 2414, 2674
PsuI RGATCY 6 cut(s) 74, 799, 913, 2108, 2154, 2282
PvuII CAGCTG 1 cut(s) 3002
RsaI GTAC 5 cut(s) 624, 1862, 2316, 2445, 2741
RsaNI GTAC 5 cut(s) 623, 1861, 2315, 2444, 2740
RseI CAYNNNNRTG 5 cut(s) 47, 507, 896, 1500, 1979
SaqAI TTAA 7 cut(s) 90, 165, 1245, 1734, 1979, 2195, 2898
SatI GCNGC 8 cut(s) 55, 183, 248, 532, 1445, 2925, 2952, 3000
Sau96I GGNCC 7 cut(s) 140, 319, 571, 1566, 1691, 2468, 2809
ScaI AGTACT 1 cut(s) 2316
SchI GAGTC 7 cut(s) 692, 929, 1207, 1244, 2444, 2634, 2933
ScrFI CCNGG 8 cut(s) 16, 905, 1372, 2508, 2611, 2907, 2917, 2918
SduI GDGCHC 3 cut(s) 850, 893, 1525
SfcI CTRYAG 3 cut(s) 1256, 1749, 1959
SfuI TTCGAA 2 cut(s) 715, 2516
SinI GGWCC 3 cut(s) 319, 571, 2468
SmaI CCCGGG 1 cut(s) 2918
SmiMI CAYNNNNRTG 5 cut(s) 47, 507, 896, 1500, 1979
SmlI CTYRAG 4 cut(s) 254, 535, 2687, 2993
SmoI CTYRAG 4 cut(s) 254, 535, 2687, 2993
SnaBI TACGTA 1 cut(s) 1864
SseBI AGGCCT 2 cut(s) 290, 2257
SsiI CCGC 2 cut(s) 680, 2329
SspI AATATT 2 cut(s) 277, 1951
StuI AGGCCT 2 cut(s) 290, 2257
StyD4I CCNGG 8 cut(s) 14, 903, 1370, 2506, 2609, 2905, 2915, 2916
StyI CCWWGG 5 cut(s) 567, 882, 1380, 2748, 2770
TaaI ACNGT 6 cut(s) 1147, 1213, 1366, 1860, 2762, 2836
TaiI ACGT 3 cut(s) 357, 1071, 1866
TaqI TCGA 5 cut(s) 715, 958, 1135, 2126, 2516
TatI WGTACW 2 cut(s) 622, 2314
TfiI GAWTC 9 cut(s) 190, 201, 1217, 1460, 2005, 2226, 2345, 2522, 2678
Tru1I TTAA 7 cut(s) 90, 165, 1245, 1734, 1979, 2195, 2898
Tru9I TTAA 7 cut(s) 90, 165, 1245, 1734, 1979, 2195, 2898
TscAI CASTG 7 cut(s) 349, 367, 560, 2236, 2665, 2765, 2839
TseFI GTSAC 2 cut(s) 358, 2840
TseI GCWGC 8 cut(s) 54, 182, 247, 531, 1444, 2924, 2951, 2999
Tsp45I GTSAC 2 cut(s) 358, 2840
TspGWI ACGGA 4 cut(s) 386, 1037, 2828, 2967
TspMI CCCGGG 1 cut(s) 2916
TspRI CASTG 7 cut(s) 349, 367, 560, 2236, 2665, 2765, 2839
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 319, 571, 2468
VspI ATTAAT 3 cut(s) 1245, 2195, 2898
XagI CCTNNNNNAGG 2 cut(s) 314, 960
XapI RAATTY 7 cut(s) 266, 587, 852, 1131, 1624, 2543, 2554
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 4 cut(s) 36, 46, 263, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 1378
XmaI CCCGGG 1 cut(s) 2916
XmiI GTMKAC 2 cut(s) 927, 1605
XmnI GAANNNNTTC 4 cut(s) 23, 120, 711, 1464
ZrmI AGTACT 1 cut(s) 2316
Zsp2I ATGCAT 1 cut(s) 1497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.