MD12G1205600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005149
Physical Location & Seq
Forward (+)
28608122 .. 28615977
7856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1205600.v1.1.491

Sequence Viewer

Length: 3003 bp
ATGTTGGAAATGGGGGTCATCTCCAGAAAGCTGTTCCCAGCGTGTGAGAGCATGTGCATATGCTGCCCTGCTATGAGGTCAAGATCTAGACAACCCGTTAAGCGTTACAAAAAGCTGCTTGCCGAAATCTTTCCCAAGTCTCCTGATTGCCCTCCCAATGAAAGAAAAATTGTTAAGTTATGCGAATATGCTGCCAAAAATCCTTTTCGAATCCCAAAGATTGCCAAATATCTTGAAGATAGATGCTACAAAGAGCTTAGATTGGAGCACATCAAATTCATCAATATTGTTGTGGAGGCTTACAACAAGTTGCTTTGCCTGTGTAAGGAGCAGATGGCATGTTTTGCAGTCAGTCTGGTGCATGTGGTCACTGAGCTCCTGGACAACTCTAAGCAAGATGCTATGCGCATACTTGGATGCCAAACCCTAACGAATTTCATCTATAGTCAGACAGACGGCACTTACACACATACCATTGAGAGTTTGGTTCACAAAGTATGTAAGCTTGCACGTCAAAGTGGTGAAGTTCATCAAAGCTTGAGAGCATCAAGTTTGCAATGCCTTTCAGCCATGGTGCGATTCATGGCAGAATTTTCGTATATTTTTGTTGATTTTGATGAGATTGTACATGTCACCTTGGATAACTATGAGCCAGATACACATAATGAAGATGATGAGAGAGGGCAGCCACATCATAATTGGGTGGATGAAGTGGTTCGAAGTGAAAGCAGAGTTGGTGTAGTTGGCGATGACGCTAGCCCTAGCTGCAAGATCATCAGGCCACGACCCGAAAAGAAGGATCCTGCTCTTCTGACAAGAGAAGAAATTGAAACACCAAAAGTGTGGGCTCAAATTTGTATTCAGAGAATGATAGAGTTATCCAAGGAGAGCACAACAATGCGCCGAGTATTGGATCCAATGTTTGTCTACTTTGATTCTGGGCACCACTGGGTTCCTCGACAAGGGTTGGCCATGCTGGTTTTGTCTGATATGTCATACTTTATGGAGGCTTCAGGGAATCAGCAGTTGATTTTAGCCTATGTGATCCGTCATCTTGACCACAAAAACATTTCGCATGATCCGCAACTCAAATCTTCTGTCATTCAAGTTGCTAGTGCTTTGGCTAGCCAGATTCGATCTGGTGCAGTGTTGGCAGAAATTGGATTTGTGTCTGACCTATGTAGGCATTTGAGGAAGAGTCTTCAAGCCACTGCTGAATCAGTTGGAGAGCAAGAATCAAACATTAACATCATGCTACAAAATTCCATTGAAGACTGCTTACTAGAAATTTCGAGAGGAATTGGCAACGTACGACCACTATTTGACATGATGGCTATAACTCTGGAGAAGCTTCCATCTGGAATTGTTGCTAGGGCAACCATTGGATCGTTAATGATTGTTGCTCACATGATCTCTTTAGCGTTGATCTCTTCACGTACACAGCAGGTGTTTCCCGAGTCCCTTCTTGTCCAACTTCTGAAAGTCATGGTGCATCCAGATGTTGAGGTCCGTGTTGGAGCACACCAGATATTTTCTATTCTTCTCATTCCAAGTTCTAACCGGTCCCGACATGAAGCTTCATCTCTACAATCTGGTTTTGGATACCAATCAAGAGGATGGCAGTCGAATACAGCATCTACCTTTGCTTCAATCACAGCCAGACTTGAAAAGCTGAGGAAGGAAAAAGATGGCCCCAAAGCCGAAAAGCACGGGAACAATAATGCTTGTGATGATTTTAAAGATAAAGAGCCTGCTGAAGAAGACTGGAAGCAGGGGCATAACCGAAAAAATTCCCCTAATTTTTACAAAATTAGCTCTATTATTGACAGGACGGCTGGAACCGTCAGCTTAATAGAGCCAGATCCGTATGTTATGAAGTTTAGTGAGGATCAAATATCACACTTGCTGTCTGCTTTTTGGATACAAGCCAATCTCCCAGATAATTTGCCTTCAAATATTGAAGCTGTAGGCCATTCTTTTAGTTTAGTGCTTATTTCTTCACATTTGAAGAATCCAACAGACAACCTTATAGTTCGTTTCATTCAATTTCTGCTGTCTCTGAGGAATATATCCCTTGATTCTAACAAAGGGACGTTCCCCCCAGCATGTCGGAGATCTATGCTTGTACTATCGATTGGCATGTTGATGTTTGCGGCTAAGATCTATCACATTCCTTTGCTTAAGTCATTAATACCATATGATATTCAGGTTGATCCATATTTGGGCATCAGTGACGATCTTCAGATTTATGTAAAGCCTAATGCAGATGTTAGTAAATATGGATCTGTTTCCGATAACCAGATGGCTTCATCATTACTATCTGATTTGCGGAACAAGATATACAAATCAGACAATATCATGATGGAAATTTTGATTCAGTTTCTATCAAAGGTTTCTGAGATGAAGGGAGAAGATGTGGCAAAGCAACTTTTGGAGTCATTCACACCTGATGATATATTCATGTTTGGCCCACAGTCAATGCTTGACTTTGACCAAAATCAAATGCCAGGCCATTCGAAGGAATCATCGTTTGATGGGGAATTTCCGACAAATTCATCAGAGGAGGATGATGCGACAAGTGAAGCATCTGTCGCTGACCTCTCTCGTTTCATTCCCAGAGTGCCTACATCCTCCTCTGTGCCGCCCCATGTTATCAGCATTGGACAGCTGATGGAATCGGCACTTGAGGTAGCCGGTCAAGTAGCGGGAACAGCTGTCTCTACATCACCTCTCCCCTACAACACCATGGCAAGCCGGTGCGAAGCCCTTGGCACAGGGACAAGGAAGAAACTCTCGAACTGGTTGGCCTATGAAAATCATCAGAGCAGTGTCCGTGACAGATTGTTTCCAGCAGTTCCTGCAGATGGACGCGCAGCACTCCAGAAGATAACGAGTGACATTGGACCTGCTCATGGAGCTGCATCGTCACAAGATCCCTGGTTGGCTATAAGGCTGCCTCCTGCTAGTCCATTTGACAACTTCCTCAAGGCAGCTGGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1001

Amino Acids

111.73

Weight (kDa)

6.32

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 68 - 161 4.5e-23 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1435
Acc16I TGCGCA 1 cut(s) 407
Acc36I ACCTGC 2 cut(s) 1435, 2914
AccB1I GGYRCC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 927
AccII CGCG 1 cut(s) 2871
AciI CCGC 5 cut(s) 1082, 2153, 2329, 2642, 2705
AcoI YGGCCR 1 cut(s) 969
AcuI CTGAAG 3 cut(s) 996, 1776, 2225
AfaI GTAC 4 cut(s) 627, 1311, 1438, 2127
AfiI CCNNNNNNNGG 7 cut(s) 325, 910, 962, 2222, 2518, 2864, 2912
AflII CTTAAG 1 cut(s) 2180
AflIII ACRYGT 1 cut(s) 628
AgeI ACCGGT 1 cut(s) 1560
AjiI CACGTC 1 cut(s) 512
AjnI CCWGG 3 cut(s) 378, 2506, 2936
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
Alw21I GWGCWC 4 cut(s) 270, 378, 893, 1522
Alw26I GTCTC 3 cut(s) 144, 2061, 2722
AlwNI CAGNNNCTG 1 cut(s) 2858
Ama87I CYCGRG 1 cut(s) 1454
AoxI GGCC 7 cut(s) 779, 969, 1690, 1969, 2467, 2509, 2805
ApeKI GCWGC 9 cut(s) 63, 115, 191, 685, 765, 2873, 2918, 2953, 2990
ArsI GACNNNNNNTTYG 2 cut(s) 1083, 1115
AseI ATTAAT 1 cut(s) 2189
AsiGI ACCGGT 1 cut(s) 1560
Asp700I GAANNNNTTC 3 cut(s) 129, 714, 1789
AspLEI GCGC 3 cut(s) 408, 903, 2873
AspS9I GGNCC 5 cut(s) 1507, 1563, 1691, 2468, 2903
AsuHPI GGTGA 3 cut(s) 533, 625, 2718
AsuII TTCGAA 3 cut(s) 208, 718, 2516
AsuNHI GCTAGC 2 cut(s) 755, 1124
AvaI CYCGRG 1 cut(s) 1454
AvaII GGWCC 3 cut(s) 1507, 1563, 2903
BaeGI GKGCMC 1 cut(s) 945
BalI TGGCCA 1 cut(s) 971
BamHI GGATCC 2 cut(s) 799, 913
BanI GGYRCC 1 cut(s) 942
BanII GRGCYC 2 cut(s) 378, 850
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BbsI GAAGAC 3 cut(s) 1193, 1278, 1767
Bbv12I GWGCWC 4 cut(s) 270, 378, 893, 1522
BbvCI CCTCAGC 1 cut(s) 1673
BbvI GCAGC 8 cut(s) 50, 102, 178, 697, 752, 2885, 2905, 2940
BceAI ACGGC 2 cut(s) 472, 1848
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 576, 610
BciT130I CCWGG 3 cut(s) 380, 2508, 2938
BciVI GTATCC 2 cut(s) 1595, 1914
BcoDI GTCTC 3 cut(s) 144, 2061, 2722
BfaI CTAG 8 cut(s) 87, 756, 762, 1113, 1125, 1283, 1371, 2964
BfmI CTRYAG 3 cut(s) 442, 1965, 2859
BfrI CTTAAG 1 cut(s) 2180
BfuAI ACCTGC 2 cut(s) 1435, 2914
BfuI GTATCC 2 cut(s) 1595, 1914
BglII AGATCT 3 cut(s) 83, 2114, 2160
Bme1390I CCNGG 3 cut(s) 380, 2508, 2938
Bme18I GGWCC 3 cut(s) 1507, 1563, 2903
BmeT110I CYCGRG 1 cut(s) 1454
BmgBI CACGTC 1 cut(s) 512
BmgT120I GGNCC 5 cut(s) 1507, 1563, 1691, 2468, 2903
BmiI GGNNCC 7 cut(s) 801, 915, 944, 954, 1565, 1693, 1840
BmrFI CCNGG 3 cut(s) 380, 2508, 2938
BmrI ACTGGG 1 cut(s) 958
BmtI GCTAGC 2 cut(s) 759, 1128
BmuI ACTGGG 1 cut(s) 958
BpiI GAAGAC 3 cut(s) 1193, 1278, 1767
BpmI CTGGAG 3 cut(s) 7, 1364, 2864
Bpu10I CCTNAGC 1 cut(s) 1673
Bpu14I TTCGAA 3 cut(s) 208, 718, 2516
BpuEI CTTGAG 3 cut(s) 559, 2705, 2969
Bsa29I ATCGAT 1 cut(s) 2132
BsaAI YACGTR 1 cut(s) 1436
BsaBI GATNNNNATC 1 cut(s) 1606
BsaJI CCNNGG 6 cut(s) 570, 636, 882, 2745, 2767, 2936
BsaWI WCCGGW 1 cut(s) 1560
BsaXI ACNNNNNCTCC 2 cut(s) 2400, 2430
Bsc4I CCNNNNNNNGG 7 cut(s) 325, 910, 962, 2222, 2518, 2864, 2912
Bse118I RCCGGY 3 cut(s) 1560, 2693, 2754
Bse1I ACTGG 3 cut(s) 953, 1769, 2804
Bse3DI GCAATG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 1606
BseBI CCWGG 3 cut(s) 380, 2508, 2938
BseCI ATCGAT 1 cut(s) 2132
BseDI CCNNGG 6 cut(s) 570, 636, 882, 2745, 2767, 2936
BseGI GGATG 6 cut(s) 422, 712, 1492, 1622, 2572, 2627
BseJI GATNNNNATC 1 cut(s) 1606
BseLI CCNNNNNNNGG 7 cut(s) 325, 910, 962, 2222, 2518, 2864, 2912
BseMI GCAATG 1 cut(s) 563
BseMII CTCAG 4 cut(s) 363, 1664, 2051, 2388
BseNI ACTGG 3 cut(s) 953, 1769, 2804
BseRI GAGGAG 2 cut(s) 2576, 2623
BseSI GKGCMC 1 cut(s) 945
BseXI GCAGC 8 cut(s) 50, 102, 178, 697, 752, 2885, 2905, 2940
BseYI CCCAGC 3 cut(s) 37, 2101, 2993
BsgI GTGCAG 1 cut(s) 1164
Bsh1236I CGCG 1 cut(s) 2871
BshFI GGCC 7 cut(s) 781, 971, 1692, 1971, 2469, 2511, 2807
BshNI GGYRCC 1 cut(s) 942
BshTI ACCGGT 1 cut(s) 1560
BshVI ATCGAT 1 cut(s) 2132
BsiHKAI GWGCWC 4 cut(s) 270, 378, 893, 1522
BsiHKCI CYCGRG 1 cut(s) 1454
BsiSI CCGG 3 cut(s) 1561, 2694, 2755
BsiWI CGTACG 1 cut(s) 1309
BslFI GGGAC 4 cut(s) 1444, 1549, 2104, 2791
BslI CCNNNNNNNGG 7 cut(s) 325, 910, 962, 2222, 2518, 2864, 2912
BsmAI GTCTC 3 cut(s) 144, 2061, 2722
BsmFI GGGAC 4 cut(s) 1444, 1549, 2104, 2791
BsnI GGCC 7 cut(s) 781, 971, 1692, 1971, 2469, 2511, 2807
BsoBI CYCGRG 1 cut(s) 1454
Bsp119I TTCGAA 3 cut(s) 208, 718, 2516
Bsp1286I GDGCHC 6 cut(s) 270, 378, 850, 893, 945, 1522
Bsp1407I TGTACA 1 cut(s) 625
Bsp19I CCATGG 2 cut(s) 570, 2745
BspACI CCGC 5 cut(s) 1082, 2153, 2329, 2642, 2705
BspANI GGCC 7 cut(s) 781, 971, 1692, 1971, 2469, 2511, 2807
BspCNI CTCAG 4 cut(s) 364, 1665, 2052, 2389
BspDI ATCGAT 1 cut(s) 2132
BspFNI CGCG 1 cut(s) 2871
BspHI TCATGA 1 cut(s) 2358
BspLI GGNNCC 7 cut(s) 801, 915, 944, 954, 1565, 1693, 1840
BspMAI CTGCAG 1 cut(s) 2863
BspMI ACCTGC 2 cut(s) 1435, 2914
BspOI GCTAGC 2 cut(s) 759, 1128
BspQI GCTCTTC 1 cut(s) 813
BspT104I TTCGAA 3 cut(s) 208, 718, 2516
BspT107I GGYRCC 1 cut(s) 942
BspTI CTTAAG 1 cut(s) 2180
BsrDI GCAATG 1 cut(s) 563
BsrFI RCCGGY 3 cut(s) 1560, 2693, 2754
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 3 cut(s) 953, 1769, 2804
BssAI RCCGGY 3 cut(s) 1560, 2693, 2754
BssECI CCNNGG 6 cut(s) 570, 636, 882, 2745, 2767, 2936
BssT1I CCWWGG 5 cut(s) 570, 636, 882, 2745, 2767
Bst2UI CCWGG 3 cut(s) 380, 2508, 2938
Bst4CI ACNGT 2 cut(s) 1843, 2475
Bst6I CTCTTC 3 cut(s) 813, 1190, 1435
BstAFI CTTAAG 1 cut(s) 2180
BstAPI GCANNNNNTGC 3 cut(s) 63, 344, 2858
BstAUI TGTACA 1 cut(s) 625
BstBAI YACGTR 1 cut(s) 1436
BstBI TTCGAA 3 cut(s) 208, 718, 2516
BstC8I GCNNGC 6 cut(s) 120, 507, 757, 1126, 1752, 2752
BstDEI CTNAG 7 cut(s) 257, 372, 390, 1673, 2060, 2157, 2397
BstDSI CCRYGG 2 cut(s) 570, 2745
BstENI CCTNNNNNAGG 2 cut(s) 323, 960
BstF5I GGATG 6 cut(s) 422, 712, 1492, 1622, 2572, 2627
BstFNI CGCG 1 cut(s) 2871
BstHHI GCGC 3 cut(s) 408, 903, 2873
BstMAI GTCTC 3 cut(s) 144, 2061, 2722
BstMWI GCNNNNNNNGC 9 cut(s) 63, 344, 765, 1081, 1151, 2591, 2711, 2858, 2915
BstNI CCWGG 3 cut(s) 380, 2508, 2938
BstNSI RCATGY 6 cut(s) 55, 342, 365, 632, 2109, 2143
BstSCI CCNGG 3 cut(s) 378, 2506, 2936
BstSFI CTRYAG 3 cut(s) 442, 1965, 2859
BstSLI GKGCMC 1 cut(s) 945
BstUI CGCG 1 cut(s) 2871
BstV1I GCAGC 8 cut(s) 50, 102, 178, 697, 752, 2885, 2905, 2940
BstV2I GAAGAC 3 cut(s) 1193, 1278, 1767
BstX2I RGATCY 8 cut(s) 83, 799, 913, 1861, 2114, 2160, 2282, 2932
BstXI CCANNNNNNTGG 1 cut(s) 843
BstYI RGATCY 8 cut(s) 83, 799, 913, 1861, 2114, 2160, 2282, 2932
Bsu15I ATCGAT 1 cut(s) 2132
BsuI GTATCC 2 cut(s) 1595, 1914
BsuRI GGCC 7 cut(s) 781, 971, 1692, 1971, 2469, 2511, 2807
BsuTUI ATCGAT 1 cut(s) 2132
BtgI CCRYGG 2 cut(s) 570, 2745
BtgZI GCGATG 1 cut(s) 762
BtrI CACGTC 1 cut(s) 512
BtsCI GGATG 6 cut(s) 422, 712, 1492, 1622, 2572, 2627
BtsI GCAGTG 3 cut(s) 1152, 1209, 2833
BtsIMutI CAGTG 6 cut(s) 369, 946, 1152, 1209, 2236, 2833
BveI ACCTGC 2 cut(s) 1435, 2914
Cac8I GCNNGC 6 cut(s) 120, 507, 757, 1126, 1752, 2752
CaiI CAGNNNCTG 1 cut(s) 2858
CciI TCATGA 1 cut(s) 2358
CfoI GCGC 3 cut(s) 408, 903, 2873
Cfr10I RCCGGY 3 cut(s) 1560, 2693, 2754
Cfr13I GGNCC 5 cut(s) 1507, 1563, 1691, 2468, 2903
ClaI ATCGAT 1 cut(s) 2132
CseI GACGC 2 cut(s) 761, 2877
Csp6I GTAC 4 cut(s) 626, 1310, 1437, 2126
CspAI ACCGGT 1 cut(s) 1560
CviQI GTAC 4 cut(s) 626, 1310, 1437, 2126
DdeI CTNAG 7 cut(s) 257, 372, 390, 1673, 2060, 2157, 2397
DraI TTTAAA 1 cut(s) 1738
EaeI YGGCCR 1 cut(s) 969
Eam1104I CTCTTC 3 cut(s) 813, 1190, 1435
EarI CTCTTC 3 cut(s) 813, 1190, 1435
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 5 cut(s) 570, 636, 882, 2745, 2767
Eco24I GRGCYC 2 cut(s) 378, 850
Eco47I GGWCC 3 cut(s) 1507, 1563, 2903
Eco53kI GAGCTC 1 cut(s) 376
Eco57I CTGAAG 3 cut(s) 996, 1776, 2225
Eco88I CYCGRG 1 cut(s) 1454
EcoICRI GAGCTC 1 cut(s) 376
EcoNI CCTNNNNNAGG 2 cut(s) 323, 960
EcoRII CCWGG 3 cut(s) 378, 2506, 2936
EcoT14I CCWWGG 5 cut(s) 570, 636, 882, 2745, 2767
EcoT38I GRGCYC 2 cut(s) 378, 850
ErhI CCWWGG 5 cut(s) 570, 636, 882, 2745, 2767
FaqI GGGAC 4 cut(s) 1444, 1549, 2104, 2791
FauI CCCGC 1 cut(s) 2698
FauNDI CATATG 2 cut(s) 59, 2197
FblI GTMKAC 1 cut(s) 927
FokI GGATG 6 cut(s) 429, 719, 1479, 1629, 2579, 2614
FriOI GRGCYC 2 cut(s) 378, 850
FspAI RTGCGCAY 1 cut(s) 407
FspBI CTAG 8 cut(s) 87, 756, 762, 1113, 1125, 1283, 1371, 2964
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 3 cut(s) 407, 902, 2872
GsaI CCCAGC 3 cut(s) 41, 2105, 2997
GsuI CTGGAG 3 cut(s) 7, 1364, 2864
HaeIII GGCC 7 cut(s) 781, 971, 1692, 1971, 2469, 2511, 2807
HapII CCGG 3 cut(s) 1561, 2694, 2755
HgaI GACGC 2 cut(s) 761, 2877
HhaI GCGC 3 cut(s) 408, 903, 2873
Hin6I GCGC 3 cut(s) 406, 901, 2871
HinP1I GCGC 3 cut(s) 406, 901, 2871
HindIII AAGCTT 4 cut(s) 503, 535, 1349, 1575
HpaII CCGG 3 cut(s) 1561, 2694, 2755
HphI GGTGA 3 cut(s) 533, 625, 2718
Hpy166II GTNNAC 3 cut(s) 490, 928, 1439
Hpy8I GTNNAC 3 cut(s) 490, 928, 1439
HpyAV CCTTC 6 cut(s) 790, 1472, 1672, 1959, 2398, 2512
HpyCH4III ACNGT 2 cut(s) 1843, 2475
HpyCH4IV ACGT 4 cut(s) 511, 1308, 1435, 2093
HpyF10VI GCNNNNNNNGC 9 cut(s) 63, 344, 765, 1081, 1151, 2591, 2711, 2858, 2915
HpyF3I CTNAG 7 cut(s) 257, 372, 390, 1673, 2060, 2157, 2397
HpySE526I ACGT 4 cut(s) 511, 1308, 1435, 2093
HspAI GCGC 3 cut(s) 406, 901, 2871
LguI GCTCTTC 1 cut(s) 813
LmnI GCTCC 5 cut(s) 265, 328, 381, 1517, 2915
Lsp1109I GCAGC 8 cut(s) 50, 102, 178, 697, 752, 2885, 2905, 2940
MaeI CTAG 8 cut(s) 87, 756, 762, 1113, 1125, 1283, 1371, 2964
MaeII ACGT 4 cut(s) 511, 1308, 1435, 2093
MaeIII GTNAC 7 cut(s) 104, 367, 631, 2231, 2834, 2894, 2925
MflI RGATCY 8 cut(s) 83, 799, 913, 1861, 2114, 2160, 2282, 2932
MhlI GDGCHC 6 cut(s) 270, 378, 850, 893, 945, 1522
MlsI TGGCCA 1 cut(s) 971
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 3 cut(s) 1207, 1466, 2444
MmeI TCCRAC 6 cut(s) 1204, 1495, 1495, 2039, 2090, 2570
Mox20I TGGCCA 1 cut(s) 971
MroXI GAANNNNTTC 3 cut(s) 129, 714, 1789
MscI TGGCCA 1 cut(s) 971
MseI TTAA 8 cut(s) 99, 174, 1245, 1391, 1737, 1850, 2181, 2189
MslI CAYNNNNRTG 3 cut(s) 896, 1497, 2144
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 3 cut(s) 2668, 2714, 2993
MspCI CTTAAG 1 cut(s) 2180
MspI CCGG 3 cut(s) 1561, 2694, 2755
MspR9I CCNGG 3 cut(s) 380, 2508, 2938
MvaI CCWGG 3 cut(s) 380, 2508, 2938
MvnI CGCG 1 cut(s) 2871
MwoI GCNNNNNNNGC 9 cut(s) 63, 344, 765, 1081, 1151, 2591, 2711, 2858, 2915
NcoI CCATGG 2 cut(s) 570, 2745
NdeI CATATG 2 cut(s) 59, 2197
NheI GCTAGC 2 cut(s) 755, 1124
NlaIV GGNNCC 7 cut(s) 801, 915, 944, 954, 1565, 1693, 1840
NmeAIII GCCGAG 1 cut(s) 929
NmuCI GTSAC 6 cut(s) 367, 631, 2231, 2834, 2894, 2925
NsbI TGCGCA 1 cut(s) 407
NspI RCATGY 6 cut(s) 55, 342, 365, 632, 2109, 2143
NspV TTCGAA 3 cut(s) 208, 718, 2516
PagI TCATGA 1 cut(s) 2358
PaqCI CACCTGC 1 cut(s) 1435
PciI ACATGT 1 cut(s) 628
PciSI GCTCTTC 1 cut(s) 813
PdmI GAANNNNTTC 3 cut(s) 129, 714, 1789
Pfl23II CGTACG 1 cut(s) 1309
PfoI TCCNGGA 1 cut(s) 378
PinAI ACCGGT 1 cut(s) 1560
PleI GAGTC 3 cut(s) 1206, 1465, 2443
PpsI GAGTC 3 cut(s) 1206, 1465, 2443
Ppu21I YACGTR 1 cut(s) 1436
PscI ACATGT 1 cut(s) 628
PshBI ATTAAT 1 cut(s) 2189
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 3 cut(s) 378, 2506, 2936
PspFI CCCAGC 3 cut(s) 37, 2101, 2993
PspGI CCWGG 3 cut(s) 378, 2506, 2936
PspLI CGTACG 1 cut(s) 1309
PspN4I GGNNCC 7 cut(s) 801, 915, 944, 954, 1565, 1693, 1840
PspPI GGNCC 5 cut(s) 1507, 1563, 1691, 2468, 2903
PsrI GAACNNNNNNTAC 2 cut(s) 2324, 2356
PstI CTGCAG 1 cut(s) 2863
PstNI CAGNNNCTG 1 cut(s) 2858
PsuI RGATCY 8 cut(s) 83, 799, 913, 1861, 2114, 2160, 2282, 2932
PvuII CAGCTG 3 cut(s) 2668, 2714, 2993
RsaI GTAC 4 cut(s) 627, 1311, 1438, 2127
RsaNI GTAC 4 cut(s) 626, 1310, 1437, 2126
RseI CAYNNNNRTG 3 cut(s) 896, 1497, 2144
SacI GAGCTC 1 cut(s) 378
SapI GCTCTTC 1 cut(s) 813
SaqAI TTAA 8 cut(s) 99, 174, 1245, 1391, 1737, 1850, 2181, 2189
Sau96I GGNCC 5 cut(s) 1507, 1563, 1691, 2468, 2903
SchI GAGTC 3 cut(s) 1207, 1466, 2444
ScrFI CCNGG 3 cut(s) 380, 2508, 2938
SduI GDGCHC 6 cut(s) 270, 378, 850, 893, 945, 1522
SfcI CTRYAG 3 cut(s) 442, 1965, 2859
SfuI TTCGAA 3 cut(s) 208, 718, 2516
SinI GGWCC 3 cut(s) 1507, 1563, 2903
SmiMI CAYNNNNRTG 3 cut(s) 896, 1497, 2144
SmlI CTYRAG 4 cut(s) 538, 2180, 2684, 2984
SmoI CTYRAG 4 cut(s) 538, 2180, 2684, 2984
SsiI CCGC 5 cut(s) 1082, 2153, 2329, 2642, 2705
SspI AATATT 2 cut(s) 286, 1957
SspMI CTAG 8 cut(s) 87, 756, 762, 1113, 1125, 1283, 1371, 2964
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 3 cut(s) 378, 2506, 2936
StyI CCWWGG 5 cut(s) 570, 636, 882, 2745, 2767
TaaI ACNGT 2 cut(s) 1843, 2475
TaiI ACGT 4 cut(s) 514, 1311, 1438, 2096
TaqI TCGA 9 cut(s) 208, 718, 958, 1135, 1292, 1625, 2132, 2516, 2795
TatI WGTACW 2 cut(s) 625, 2125
TauI GCSGC 2 cut(s) 2156, 2644
Tru1I TTAA 8 cut(s) 99, 174, 1245, 1391, 1737, 1850, 2181, 2189
Tru9I TTAA 8 cut(s) 99, 174, 1245, 1391, 1737, 1850, 2181, 2189
TscAI CASTG 6 cut(s) 376, 953, 1152, 1216, 2236, 2833
TseFI GTSAC 6 cut(s) 367, 631, 2231, 2834, 2894, 2925
TseI GCWGC 9 cut(s) 63, 115, 191, 685, 765, 2873, 2918, 2953, 2990
Tsp45I GTSAC 6 cut(s) 367, 631, 2231, 2834, 2894, 2925
TspGWI ACGGA 4 cut(s) 1037, 1499, 1854, 2822
TspRI CASTG 6 cut(s) 376, 953, 1152, 1216, 2236, 2833
Vha464I CTTAAG 1 cut(s) 2180
VpaK11BI GGWCC 3 cut(s) 1507, 1563, 2903
VspI ATTAAT 1 cut(s) 2189
XagI CCTNNNNNAGG 2 cut(s) 323, 960
XbaI TCTAGA 1 cut(s) 86
XceI RCATGY 6 cut(s) 55, 342, 365, 632, 2109, 2143
XcmI CCANNNNNNNNNTGG 3 cut(s) 481, 696, 1136
XmiI GTMKAC 1 cut(s) 927
XmnI GAANNNNTTC 3 cut(s) 129, 714, 1789
XspI CTAG 8 cut(s) 87, 756, 762, 1113, 1125, 1283, 1371, 2964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.