RLG00000025246

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005149
Physical Location & Seq
Forward (+)
43359370 .. 43366190
6821 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025246

Sequence Viewer

Length: 3015 bp
ATGGGTATCATATCCAGGAAGATTTTCCCAGCATGTGGGAACATGTGTGTATGCTGCCCTGCTATGAGGTCAAGATCTAGACAACCTGTTAAGCGTTACAAAAAACTGCTTGCAGAAATCTTTCCCAAGTCTCCTGATGGCCCTCCCAATGAAAGGAAAATTGTTAAGTTATGCGAATATGCTGCAAAGAATCCTTTTAGAATCCCTAAGATTGCCAAACATCTTGAAGATAGGTGTTACAAAGAGCTGCGACTTGAGCATGTCAAATTCATCAATATTGTTGCAGAGGCCTACAACAAGTTACTTTGCCTCTGTAAGGACCAGATGGCTTATTTTGCTGTCAGTGTGCTAAACGTAGTCACTGAGCTTCTGGACAACTCTAAGCAAGATGCTGTCCGTATAATTGGATGCCAAACTCTGACGAGGTTCATCTACACTCAGGCAGATGGAACTTATACACACAACATTGAGAGTTTGGTTCACAAAGTATGTAAGCTGGCACATGAGAGAGGTGAAGACCATCAAAAGCGCTGCTTGAGAGCATCAAGTTTGCAGTGCCTTTCAGCCATGGTCCAATTCATGACTGAATTTTCATATATTTTTGTTGATTTTGATGAGATTGTGCATGCCACTTTGGATAACTATGAGCCTGATGCAAATGATGATGATGATGATGATGAGGGAGGGGAGTCGCATCATAATTGGGTGGATGAAGTAGTTCGAAGTGAAGGTAGAGTTGGTGCAATTGCCGGTGGGTATTCTAGCCCTAGTTATAAGGTCATCAGGCCACGGCCAGAAAAAAAGGATCCTTCTCTTCTCACAAGAGAAGAGACAGAGACGCCCAACATTTGGGCTCAAATTTGTATCCAGAGAATGATTGAATTAGCCAAGGAGAGCACAACAATGCGCCGGGTATTGGATCCAGTGTTTGTCTACTTTGACTCCAGGCACCATTGGGTTCCTCGACAAGGGTTGGCTTTGTCAGTTTTGTCTGATATGTCATACTTTTTGGAGACTTCAGGAAATCAGCAGATGATTTTAGCCTATGCAATCCGTCATCTTGACCACAAAAACGTTTCACATGATCCACAACTCAAATCTCAGATCATTCAAGTTGCTAGTGCTTTGGCAAGCCAAATTCGATCTGGAACCGTGCTAGAAGAAATTGGTTTTGTCTCTGACCTATGCAGGCATTTGAGGAAAAGTCTTCAAGCTACTGTTGAATCAGTTGGAGAGCAAGAGTCTAACATTAATACCATGCTACAGAGTTCCATTGAAGATTGCTTATTAGAAATTGCTAGAGGGATTGGAAATATAAGACCATTGTTTGACATGATGTCTATATCTCTGGAGAAGCTTCCATCTGGAACTGTTGCCAGGGCGACCATGGGATCATTGATGATTGTTGCTCACATGATCTCATTAGCATTGATCTCTTCACATTCACAGCAGGCGTTTCCTGAATCCCTTCTTGTCCAGCTTTTGAAAGTGATGATGCATCCAGATGTTGAGGTGCGTGTTGGAGCACACCAGATATTTTCTGTTCTTCTCATTCCATGTTCTAATCGGCCCCGACATGAAGTTGCTCCTCTACAATCTGGTTATGTATACCAGTCAAGAAAACGGAATTCAAATACAGCGTCTATTGCTTCAATTACGGCTAGACTTGAAAAGCTTAGGAGGGAAAAAGATGGCCCCAAAGCAGAAAATCAGGAGACTGATGCTCGTGATGATTTTAAAGATAGAGATACTGCAGAAGAAGATTGCAAGCAAGGGCATACCCACAAGAGTTCCCCAAACTTCTATAAAATTAGCTCTATTATTGACAAGACTGCTGGGTCAAGCCTAACAGAGCCTGAACCGTATGTAATGAAGTTTAGTGAGGATCAAATAGCAGACTTGCTGTCTGCTTTTTGGATACAAGCCAATCTCCCAGATAATTTGCCTTCAAATATTGAAGCTATAGCTCATTCTTTCATTTTAATGATAGTTTCTTCTCATTTGAAGAATCCAAATGGCAACCTTATGGTTCATTCCTTTCAATTTCTGCTATCTCTGAGGAATATCTCCCTTGATCCTAACAATGGGATGTTGCCTCCAGCTTGCCAGAGATCTATCCTTGTGCTATCGATTGGCCTGTTGATGTTTTCTGCTCAGATCTATAACATTCCTAATCTAAATGATCTGCTCAAATCATTAATACCATCTGATGTTGATCCATATTTGGGAATCAGTGATGACCTTCAAGTTTTTCTAAGGCCTGAGGCAGACATCACAAAATATGGATCTGTTATCGATAACCAGCTAGCTTCATCATTACTATTTGATTTGCGGAAAAAATTATATGAATCTGACCATGTCATAAGGGAAATTTTAGTTCAATTTCTATCAGACATTACTGAGATGGAGGCAGAGGCTGTGGCTAACCAACTTTTGGAGTCATTCGTACCTGATGATGCATTCGTGTTTGGTCCACAATCGATGCTTGACTTTGATGAAAATCAAATGCCAGGCCATTCGAAAGAATCACTTTCCTTTGATGAGGAATTTCCGACAAATTCATCAGTTGAGGATGATGCAACAAGTGAAGCATCAGTTGTTGCTGACTTCTCCCGGTTCATTCCTAGAGTCCCCTCATCCACTTCTGTCTCCCATGTTATCAGTGTTGGACAGCTTCTGGAATCAGCACTTGAGGTTGCTGGTCAAGTAGCAGGAACATCTGTATCTACATCACCTCTCCCATACAACACCATGGCAAGACAGTGTGAAGCCCTTGGCACAGGCACAAGGAAGAAGCTTTCTAATTGGTTGGCCCATGAACATCATCAAAGCAACACTGTCCGCGACATTTTGTCTCCACCATTTCTTGCAGGTGGATGCACAGCACTCCAGAAGTTGATTAATGAACCTGGGGTTACCCAGGGAGCTGCATTGGCACAGGACTCGTGGTTAGCTGCTATCAGGTTGCCTCCTGCTAGTCCGTTTGACAACTTCCTTAAGGCAGCTGGTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1005

Amino Acids

111.97

Weight (kDa)

5.66

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFR3_ARM PF21052 65 - 159 3.1e-21 EFR3, armadillo repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 774
AarI CACCTGC 1 cut(s) 2861
AasI GACNNNNNNGTC 1 cut(s) 1837
Acc36I ACCTGC 1 cut(s) 2861
AccB1I GGYRCC 1 cut(s) 948
AccB7I CCANNNNNTGG 3 cut(s) 35, 849, 2434
AccI GTMKAC 2 cut(s) 933, 1608
AccII CGCG 1 cut(s) 2844
AciI CCGC 2 cut(s) 2332, 2842
AclI AACGTT 1 cut(s) 1074
AcoI YGGCCR 1 cut(s) 791
AcsI RAATTY 8 cut(s) 266, 587, 858, 1137, 1627, 2370, 2546, 2557
AcuI CTGAAG 1 cut(s) 1002
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 1 cut(s) 2448
AfeI AGCGCT 1 cut(s) 530
AfiI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 849, 916, 968, 2084, 2225, 2434
AflII CTTAAG 1 cut(s) 2996
AflIII ACRYGT 1 cut(s) 42
AhdI GACNNNNNGTC 3 cut(s) 1336, 1903, 2851
AjnI CCWGG 6 cut(s) 14, 944, 1376, 2509, 2908, 2919
AjuI GAANNNNNNNTTGG 2 cut(s) 720, 752
Alw21I GWGCWC 2 cut(s) 899, 1528
Alw26I GTCTC 8 cut(s) 135, 824, 830, 1007, 1180, 1709, 2653, 2859
AlwNI CAGNNNCTG 3 cut(s) 1856, 2417, 2677
Aor51HI AGCGCT 1 cut(s) 530
ApeKI GCWGC 7 cut(s) 54, 182, 247, 531, 2927, 2954, 3002
ApoI RAATTY 8 cut(s) 266, 587, 858, 1137, 1627, 2370, 2546, 2557
ArsI GACNNNNNNTTYG 2 cut(s) 1625, 1657
AseI ATTAAT 3 cut(s) 1251, 2198, 2901
Asp700I GAANNNNTTC 4 cut(s) 23, 120, 717, 1467
AspLEI GCGC 2 cut(s) 531, 909
AspS9I GGNCC 7 cut(s) 140, 319, 571, 1569, 1694, 2471, 2812
AsuC2I CCSGG 2 cut(s) 911, 2614
AsuHPI GGTGA 2 cut(s) 524, 2724
AsuII TTCGAA 2 cut(s) 721, 2519
AsuNHI GCTAGC 1 cut(s) 2305
AvaII GGWCC 3 cut(s) 319, 571, 2471
AxyI CCTNAGG 1 cut(s) 2262
BaeI ACNNNNGTAYC 2 cut(s) 2706, 2739
BamHI GGATCC 2 cut(s) 805, 919
BanI GGYRCC 1 cut(s) 948
BanII GRGCYC 1 cut(s) 856
BauI CACGAG 2 cut(s) 1725, 2944
BbsI GAAGAC 2 cut(s) 522, 1199
Bbv12I GWGCWC 2 cut(s) 899, 1528
BbvI GCAGC 6 cut(s) 41, 169, 234, 518, 2914, 2941
BccI CCATC 8 cut(s) 131, 319, 440, 528, 1369, 1685, 2212, 2398
BceAI ACGGC 2 cut(s) 806, 1674
BcgI CGANNNNNNTGC 4 cut(s) 164, 198, 2925, 2959
BciT130I CCWGG 6 cut(s) 16, 946, 1378, 2511, 2910, 2921
BciVI GTATCC 2 cut(s) 875, 1911
BcnI CCSGG 2 cut(s) 911, 2614
BcoDI GTCTC 8 cut(s) 135, 824, 830, 1007, 1180, 1709, 2653, 2859
BfmI CTRYAG 3 cut(s) 1262, 1752, 1962
BfoI RGCGCY 1 cut(s) 532
BfrI CTTAAG 1 cut(s) 2996
BfuAI ACCTGC 1 cut(s) 2861
BfuI GTATCC 2 cut(s) 875, 1911
BglII AGATCT 3 cut(s) 74, 2111, 2157
BisI GCNGC 7 cut(s) 55, 183, 248, 532, 2928, 2955, 3003
BlsI GCNGC 7 cut(s) 56, 184, 249, 533, 2929, 2956, 3004
Bme1390I CCNGG 8 cut(s) 16, 911, 946, 1378, 2511, 2614, 2910, 2921
Bme18I GGWCC 3 cut(s) 319, 571, 2471
BmeRI GACNNNNNGTC 3 cut(s) 1336, 1903, 2851
BmgT120I GGNCC 7 cut(s) 140, 319, 571, 1569, 1694, 2471, 2812
BmiI GGNNCC 7 cut(s) 807, 921, 950, 960, 1150, 1571, 1696
BmrFI CCNGG 8 cut(s) 16, 911, 946, 1378, 2511, 2614, 2910, 2921
BmtI GCTAGC 1 cut(s) 2309
BpiI GAAGAC 2 cut(s) 522, 1199
BpmI CTGGAG 4 cut(s) 928, 1370, 2082, 2873
Bpu10I CCTNAGC 1 cut(s) 1676
Bpu14I TTCGAA 2 cut(s) 721, 2519
BpuEI CTTGAG 3 cut(s) 275, 556, 2711
BpuMI CCSGG 2 cut(s) 911, 2614
Bsa29I ATCGAT 3 cut(s) 2129, 2295, 2480
BsaBI GATNNNNATC 2 cut(s) 2214, 2499
BsaHI GRCGYC 1 cut(s) 839
BsaXI ACNNNNNCTCC 2 cut(s) 926, 956
Bsc4I CCNNNNNNNGG 9 cut(s) 35, 153, 316, 849, 916, 968, 2084, 2225, 2434
Bse118I RCCGGY 1 cut(s) 749
Bse1I ACTGG 2 cut(s) 923, 1612
Bse21I CCTNAGG 1 cut(s) 2262
Bse8I GATNNNNATC 2 cut(s) 2214, 2499
BseBI CCWGG 6 cut(s) 16, 946, 1378, 2511, 2910, 2921
BseCI ATCGAT 3 cut(s) 2129, 2295, 2480
BseGI GGATG 7 cut(s) 413, 715, 1498, 2094, 2578, 2636, 2882
BseJI GATNNNNATC 2 cut(s) 2214, 2499
BseLI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 849, 916, 968, 2084, 2225, 2434
BseMII CTCAG 7 cut(s) 354, 452, 1115, 2048, 2168, 2253, 2391
BseNI ACTGG 2 cut(s) 923, 1612
BseRI GAGGAG 1 cut(s) 1578
BseXI GCAGC 6 cut(s) 41, 169, 234, 518, 2914, 2941
BseYI CCCAGC 2 cut(s) 28, 1835
Bsh1236I CGCG 1 cut(s) 2844
BshNI GGYRCC 1 cut(s) 948
BshVI ATCGAT 3 cut(s) 2129, 2295, 2480
BsiHKAI GWGCWC 2 cut(s) 899, 1528
BsiSI CCGG 3 cut(s) 750, 910, 2614
BslFI GGGAC 1 cut(s) 2615
BslI CCNNNNNNNGG 9 cut(s) 35, 153, 316, 849, 916, 968, 2084, 2225, 2434
BsmAI GTCTC 8 cut(s) 135, 824, 830, 1007, 1180, 1709, 2653, 2859
BsmBI CGTCTC 1 cut(s) 830
BsmFI GGGAC 1 cut(s) 2615
BsmI GAATGC 1 cut(s) 2459
Bsp119I TTCGAA 2 cut(s) 721, 2519
Bsp1286I GDGCHC 3 cut(s) 856, 899, 1528
Bsp19I CCATGG 3 cut(s) 567, 1386, 2751
BspACI CCGC 2 cut(s) 2332, 2842
BspCNI CTCAG 7 cut(s) 355, 451, 1114, 2049, 2167, 2254, 2392
BspDI ATCGAT 3 cut(s) 2129, 2295, 2480
BspFNI CGCG 1 cut(s) 2844
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 7 cut(s) 807, 921, 950, 960, 1150, 1571, 1696
BspMAI CTGCAG 1 cut(s) 1756
BspMI ACCTGC 1 cut(s) 2861
BspOI GCTAGC 1 cut(s) 2309
BspT104I TTCGAA 2 cut(s) 721, 2519
BspT107I GGYRCC 1 cut(s) 948
BspTI CTTAAG 1 cut(s) 2996
BsrFI RCCGGY 1 cut(s) 749
BsrI ACTGG 2 cut(s) 923, 1612
BssAI RCCGGY 1 cut(s) 749
BssNAI GTATAC 1 cut(s) 1609
BssNI GRCGYC 1 cut(s) 839
BssSI CACGAG 2 cut(s) 1725, 2944
BssT1I CCWWGG 5 cut(s) 567, 888, 1386, 2751, 2773
Bst1107I GTATAC 1 cut(s) 1609
Bst2BI CACGAG 2 cut(s) 1725, 2944
Bst2UI CCWGG 6 cut(s) 16, 946, 1378, 2511, 2910, 2921
Bst4CI ACNGT 6 cut(s) 1153, 1219, 1372, 1863, 2763, 2839
Bst6I CTCTTC 3 cut(s) 819, 822, 1441
BstACI GRCGYC 1 cut(s) 839
BstAFI CTTAAG 1 cut(s) 2996
BstBI TTCGAA 2 cut(s) 721, 2519
BstC8I GCNNGC 9 cut(s) 111, 498, 627, 1132, 1190, 1452, 1769, 2104, 2307
BstDSI CCRYGG 4 cut(s) 567, 788, 1386, 2751
BstEII GGTNACC 1 cut(s) 2914
BstENI CCTNNNNNAGG 2 cut(s) 314, 966
BstF5I GGATG 7 cut(s) 413, 715, 1498, 2094, 2578, 2636, 2882
BstFNI CGCG 1 cut(s) 2844
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 2 cut(s) 531, 909
BstMAI GTCTC 8 cut(s) 135, 824, 830, 1007, 1180, 1709, 2653, 2859
BstMWI GCNNNNNNNGC 4 cut(s) 256, 335, 1646, 2933
BstNI CCWGG 6 cut(s) 16, 946, 1378, 2511, 2910, 2921
BstNSI RCATGY 4 cut(s) 36, 46, 263, 629
BstPI GGTNACC 1 cut(s) 2914
BstSCI CCNGG 8 cut(s) 14, 909, 944, 1376, 2509, 2612, 2908, 2919
BstSFI CTRYAG 3 cut(s) 1262, 1752, 1962
BstUI CGCG 1 cut(s) 2844
BstV1I GCAGC 6 cut(s) 41, 169, 234, 518, 2914, 2941
BstV2I GAAGAC 2 cut(s) 522, 1199
BstX2I RGATCY 6 cut(s) 74, 805, 919, 2111, 2157, 2285
BstYI RGATCY 6 cut(s) 74, 805, 919, 2111, 2157, 2285
BstZ17I GTATAC 1 cut(s) 1609
Bsu15I ATCGAT 3 cut(s) 2129, 2295, 2480
Bsu36I CCTNAGG 1 cut(s) 2262
BsuI GTATCC 2 cut(s) 875, 1911
BsuTUI ATCGAT 3 cut(s) 2129, 2295, 2480
BtgI CCRYGG 4 cut(s) 567, 788, 1386, 2751
BtsCI GGATG 7 cut(s) 413, 715, 1498, 2094, 2578, 2636, 2882
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 8 cut(s) 349, 360, 560, 930, 2239, 2668, 2768, 2835
BveI ACCTGC 1 cut(s) 2861
Cac8I GCNNGC 9 cut(s) 111, 498, 627, 1132, 1190, 1452, 1769, 2104, 2307
CaiI CAGNNNCTG 3 cut(s) 1856, 2417, 2677
CciI TCATGA 1 cut(s) 579
CfoI GCGC 2 cut(s) 531, 909
Cfr10I RCCGGY 1 cut(s) 749
Cfr13I GGNCC 7 cut(s) 140, 319, 571, 1569, 1694, 2471, 2812
ClaI ATCGAT 3 cut(s) 2129, 2295, 2480
CseI GACGC 2 cut(s) 847, 1629
Csp6I GTAC 1 cut(s) 2447
CviQI GTAC 1 cut(s) 2447
DraI TTTAAA 1 cut(s) 1738
DrdI GACNNNNNNGTC 1 cut(s) 1837
DriI GACNNNNNGTC 3 cut(s) 1336, 1903, 2851
DseDI GACNNNNNNGTC 1 cut(s) 1837
EaeI YGGCCR 1 cut(s) 791
Eam1104I CTCTTC 3 cut(s) 819, 822, 1441
Eam1105I GACNNNNNGTC 3 cut(s) 1336, 1903, 2851
EarI CTCTTC 3 cut(s) 819, 822, 1441
Eco130I CCWWGG 5 cut(s) 567, 888, 1386, 2751, 2773
Eco147I AGGCCT 2 cut(s) 290, 2260
Eco24I GRGCYC 1 cut(s) 856
Eco47I GGWCC 3 cut(s) 319, 571, 2471
Eco47III AGCGCT 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 1002
Eco81I CCTNAGG 1 cut(s) 2262
Eco91I GGTNACC 1 cut(s) 2914
EcoNI CCTNNNNNAGG 2 cut(s) 314, 966
EcoO65I GGTNACC 1 cut(s) 2914
EcoRI GAATTC 1 cut(s) 1627
EcoRII CCWGG 6 cut(s) 14, 944, 1376, 2509, 2908, 2919
EcoT14I CCWWGG 5 cut(s) 567, 888, 1386, 2751, 2773
EcoT22I ATGCAT 2 cut(s) 1500, 2461
EcoT38I GRGCYC 1 cut(s) 856
ErhI CCWWGG 5 cut(s) 567, 888, 1386, 2751, 2773
Esp3I CGTCTC 1 cut(s) 830
FalI AAGNNNNNCTT 4 cut(s) 518, 550, 2514, 2546
FaqI GGGAC 1 cut(s) 2615
FblI GTMKAC 2 cut(s) 933, 1608
Fnu4HI GCNGC 7 cut(s) 55, 183, 248, 532, 2928, 2955, 3003
FokI GGATG 7 cut(s) 420, 722, 1485, 2101, 2585, 2623, 2889
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 7 cut(s) 55, 183, 248, 532, 2928, 2955, 3003
GlaI GCGC 2 cut(s) 530, 908
GluI GCNGC 7 cut(s) 55, 183, 248, 532, 2928, 2955, 3003
GsaI CCCAGC 2 cut(s) 32, 1839
GsuI CTGGAG 4 cut(s) 928, 1370, 2082, 2873
HaeII RGCGCY 1 cut(s) 532
HapII CCGG 3 cut(s) 750, 910, 2614
HgaI GACGC 2 cut(s) 847, 1629
HhaI GCGC 2 cut(s) 531, 909
Hin1I GRCGYC 1 cut(s) 839
Hin6I GCGC 2 cut(s) 529, 907
HinP1I GCGC 2 cut(s) 529, 907
HindIII AAGCTT 3 cut(s) 1355, 1673, 2795
HpaII CCGG 3 cut(s) 750, 910, 2614
HphI GGTGA 2 cut(s) 524, 2724
Hpy166II GTNNAC 4 cut(s) 481, 934, 1609, 2474
Hpy8I GTNNAC 4 cut(s) 481, 934, 1609, 2474
HpyAV CCTTC 5 cut(s) 722, 819, 1478, 1956, 2252
HpyCH4III ACNGT 6 cut(s) 1153, 1219, 1372, 1863, 2763, 2839
HpyCH4IV ACGT 2 cut(s) 354, 1074
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 335, 1646, 2933
HpySE526I ACGT 2 cut(s) 354, 1074
Hsp92I GRCGYC 1 cut(s) 839
HspAI GCGC 2 cut(s) 529, 907
LmnI GCTCC 3 cut(s) 1523, 1591, 2924
Lsp1109I GCAGC 6 cut(s) 41, 169, 234, 518, 2914, 2941
MaeII ACGT 2 cut(s) 354, 1074
MaeIII GTNAC 5 cut(s) 95, 236, 300, 358, 2914
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 6 cut(s) 74, 805, 919, 2111, 2157, 2285
MhlI GDGCHC 3 cut(s) 856, 899, 1528
MlyI GAGTC 6 cut(s) 698, 935, 1250, 2447, 2637, 2936
MmeI TCCRAC 4 cut(s) 1210, 1501, 2576, 2647
Mph1103I ATGCAT 2 cut(s) 1500, 2461
MroXI GAANNNNTTC 4 cut(s) 23, 120, 717, 1467
MseI TTAA 8 cut(s) 90, 165, 1251, 1737, 1982, 2198, 2901, 2997
MslI CAYNNNNRTG 3 cut(s) 902, 1503, 1982
MspA1I CMGCKG 1 cut(s) 3005
MspCI CTTAAG 1 cut(s) 2996
MspI CCGG 3 cut(s) 750, 910, 2614
MspR9I CCNGG 8 cut(s) 16, 911, 946, 1378, 2511, 2614, 2910, 2921
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 2459
MvaI CCWGG 6 cut(s) 16, 946, 1378, 2511, 2910, 2921
MvnI CGCG 1 cut(s) 2844
MwoI GCNNNNNNNGC 4 cut(s) 256, 335, 1646, 2933
NciI CCSGG 2 cut(s) 911, 2614
NcoI CCATGG 3 cut(s) 567, 1386, 2751
NheI GCTAGC 1 cut(s) 2305
NlaIV GGNNCC 7 cut(s) 807, 921, 950, 960, 1150, 1571, 1696
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 2 cut(s) 1500, 2461
NspI RCATGY 4 cut(s) 36, 46, 263, 629
NspV TTCGAA 2 cut(s) 721, 2519
PaeI GCATGC 1 cut(s) 629
PagI TCATGA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 2861
PasI CCCWGGG 1 cut(s) 2920
PceI AGGCCT 2 cut(s) 290, 2260
PciI ACATGT 1 cut(s) 42
PctI GAATGC 1 cut(s) 2459
PdmI GAANNNNTTC 4 cut(s) 23, 120, 717, 1467
PfeI GAWTC 9 cut(s) 190, 201, 1223, 1463, 2008, 2229, 2348, 2525, 2681
PflFI GACNNNGTC 1 cut(s) 2357
PflMI CCANNNNNTGG 3 cut(s) 35, 849, 2434
PfoI TCCNGGA 1 cut(s) 14
PkrI GCNGC 7 cut(s) 56, 184, 249, 533, 2929, 2956, 3004
PleI GAGTC 6 cut(s) 697, 935, 1249, 2446, 2636, 2936
PpsI GAGTC 6 cut(s) 697, 935, 1249, 2446, 2636, 2936
PscI ACATGT 1 cut(s) 42
PshBI ATTAAT 3 cut(s) 1251, 2198, 2901
PsiI TTATAA 1 cut(s) 774
Psp1406I AACGTT 1 cut(s) 1074
Psp6I CCWGG 6 cut(s) 14, 944, 1376, 2509, 2908, 2919
PspEI GGTNACC 1 cut(s) 2914
PspFI CCCAGC 2 cut(s) 28, 1835
PspGI CCWGG 6 cut(s) 14, 944, 1376, 2509, 2908, 2919
PspN4I GGNNCC 7 cut(s) 807, 921, 950, 960, 1150, 1571, 1696
PspPI GGNCC 7 cut(s) 140, 319, 571, 1569, 1694, 2471, 2812
PstI CTGCAG 1 cut(s) 1756
PstNI CAGNNNCTG 3 cut(s) 1856, 2417, 2677
PsuI RGATCY 6 cut(s) 74, 805, 919, 2111, 2157, 2285
PsyI GACNNNGTC 1 cut(s) 2357
PvuII CAGCTG 1 cut(s) 3005
RsaI GTAC 1 cut(s) 2448
RsaNI GTAC 1 cut(s) 2447
RseI CAYNNNNRTG 3 cut(s) 902, 1503, 1982
SaqAI TTAA 8 cut(s) 90, 165, 1251, 1737, 1982, 2198, 2901, 2997
SatI GCNGC 7 cut(s) 55, 183, 248, 532, 2928, 2955, 3003
Sau96I GGNCC 7 cut(s) 140, 319, 571, 1569, 1694, 2471, 2812
SchI GAGTC 6 cut(s) 698, 935, 1250, 2447, 2637, 2936
ScrFI CCNGG 8 cut(s) 16, 911, 946, 1378, 2511, 2614, 2910, 2921
SduI GDGCHC 3 cut(s) 856, 899, 1528
SfcI CTRYAG 3 cut(s) 1262, 1752, 1962
SfuI TTCGAA 2 cut(s) 721, 2519
SinI GGWCC 3 cut(s) 319, 571, 2471
SmiMI CAYNNNNRTG 3 cut(s) 902, 1503, 1982
SmlI CTYRAG 4 cut(s) 254, 535, 2690, 2996
SmoI CTYRAG 4 cut(s) 254, 535, 2690, 2996
SphI GCATGC 1 cut(s) 629
SseBI AGGCCT 2 cut(s) 290, 2260
SsiI CCGC 2 cut(s) 2332, 2842
SspI AATATT 2 cut(s) 277, 1954
StuI AGGCCT 2 cut(s) 290, 2260
StyD4I CCNGG 8 cut(s) 14, 909, 944, 1376, 2509, 2612, 2908, 2919
StyI CCWWGG 5 cut(s) 567, 888, 1386, 2751, 2773
TaaI ACNGT 6 cut(s) 1153, 1219, 1372, 1863, 2763, 2839
TaiI ACGT 2 cut(s) 357, 1077
TaqI TCGA 7 cut(s) 721, 964, 1141, 2129, 2295, 2480, 2519
TfiI GAWTC 9 cut(s) 190, 201, 1223, 1463, 2008, 2229, 2348, 2525, 2681
Tru1I TTAA 8 cut(s) 90, 165, 1251, 1737, 1982, 2198, 2901, 2997
Tru9I TTAA 8 cut(s) 90, 165, 1251, 1737, 1982, 2198, 2901, 2997
TscAI CASTG 8 cut(s) 349, 367, 560, 930, 2239, 2668, 2768, 2842
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 7 cut(s) 54, 182, 247, 531, 2927, 2954, 3002
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 4 cut(s) 386, 1043, 1639, 2970
TspRI CASTG 8 cut(s) 349, 367, 560, 930, 2239, 2668, 2768, 2842
Tth111I GACNNNGTC 1 cut(s) 2357
Van91I CCANNNNNTGG 3 cut(s) 35, 849, 2434
Vha464I CTTAAG 1 cut(s) 2996
VpaK11BI GGWCC 3 cut(s) 319, 571, 2471
VspI ATTAAT 3 cut(s) 1251, 2198, 2901
XagI CCTNNNNNAGG 2 cut(s) 314, 966
XapI RAATTY 8 cut(s) 266, 587, 858, 1137, 1627, 2370, 2546, 2557
XbaI TCTAGA 1 cut(s) 77
XceI RCATGY 4 cut(s) 36, 46, 263, 629
XcmI CCANNNNNNNNNTGG 2 cut(s) 1142, 1384
XmiI GTMKAC 2 cut(s) 933, 1608
XmnI GAANNNNTTC 4 cut(s) 23, 120, 717, 1467
Zsp2I ATGCAT 2 cut(s) 1500, 2461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.