Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001186

Physical Location & Seq

Genomic Coordinates Reverse (-)

30152032 .. 30155444

Gene Structure 3413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1220500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGAATGGTCTCTTACATCTTGAACCAGATTACTGTGAGTTTGTTGAAGTTGATCCAACTGGGAGATATGGCAGATACAATGAAATCCTTGGCAAAGGAGCTTCTAAGATAGTTTACAGAGCATTTGATGAATATGAAGGGATAGAAGTTGCTTGGAACCAGGTCAAGCTTTATGATTTCCTGCAGAGCCCTGAAGATCTTGAGAGGCTCTACTGTGAAATCCACCTCCTCAAGACATTGAAGCACAGAAATATCATGAAATTTTACACTTCCTGGGTTGATACTGCCAACAGAAACATCAATTTTGTCACTGAAATGTTCACCTCTGGGACCCTTAAGCAGTATAGGTTAAAGCACAAGAGAGTGAACATTAGAGCAGTGAAGAATTGGTGTAGGCAGATCTTGAGAGGGCTTCTGTATCTCCATAGCCATGACCCTCCTGTGATCCATAGAGATCTTAAGTGTGACAACATCTTTATCAATGGAAACCAAGGGGAAGTGAAGATTGGTGACCTTGGTTTAGCTGCAATCTTAAGGAAATCGCACGCCGCTCACTGCGTTGGGACACCAGAGTTCATGGCTCCTGAAGTATATGAAGAGGCATATAATGAGCTAGTTGACATCTATTCATTTGGGATGTGCGTTCTGGAAATGGTCACTTTCGAATATCCGTATAGTGAATGCACTCATCCTGCTCAGATCTACAAGAAAGTTGTTTCTGGTAAAAAACCAGATGCTTTGTACAAAGTTAAAGATCCAGAAGTACGAGAATTTGTTGAGAAGTGTCTGGCAACTGCGTCGTTGAGGCTCTCTGCGAGGGAGCTTTTAAACGACCGTTTTCTCCAAATTGATGAATGTGAATATGATTTGAGATCACTAGATTATGGTAGGCACCTAGATGACATGGGTCCTCTTGCAAGGCAGCCTCTATTAGAGCTTCATCGAAGCAACAGTTCCTTTAGTAATGGATACTCGAATGATTATGATTTTGATGCCCCAAATGAGTGGACTGTTGATATAGAATCAACTGGAATCGAACTGTTTGAGCACCATGAGGATGATCATTCTGATGATGTCGACATTAGTATTAAAGGGAAGAGAGGAGAAGACGGTGGCATCTTTTTGAGACTTCGAATTACAGATAAAGAAGGTCGTGTACGCAACATTTATTTCCCATTTGACACTGAGAACGATACAGCATTGAGTGTGGCAACTGAAATGGTTGCAGAGTTGGATATCACCGATCAGGATGAAACCAGAATAGCAAATATGATTGATGGGGAAATTTCCATGTTGGTGCCCGATTGGCAATCAGGACCAGGACTAGAGGAAACACCCCGGTTTGCTAATCAAAGCTTTTGTCACAATTGTGTTTCCAATCACACCTCTAGTGGTTCCATGATGGAGTATCTGTCGAACCACCCTAGCGCTAAGGACTTGCTAGCTCTTCAATGTTGTAAACATGGTTGTCCTTCAATGCACGGTCGTTTTGAGGAGATTACGTACCAAGCTGACGAGTCTGAGCATCATGTGACAAAGGGTGAACCAAATGAATCAGGCCAATCAGACTGCGTTCAATACCAAGAAATTTGGGGACAGCAACAACACGAAAGTCGTGAACTTAGTTTAGTAGGCTCGGGACAGATCCATTCTGACGAAGAATATGAGAAAATGGATCGCTTAGTCACAGTTGAAGATGAGCAAGATTCTAAAGTGGAAAACAATACAGCATCCGAAGAAAGAAATTCCATCAGAAACTTATCAGGTTTACATTCTATTTGCATGAGCCCGCCAATGTACCATGACCTCCCAGACAGTTACGAGAATGGAATTCAGAAGGATTTGAGATGGTTCAAAGCAAAGCACCAGATGGAGCTGAGGGAGCTTCGAGATGAGCAATTAGGAATCATCTCAACATCTTTGCCGAATGCAAGTAAAGAACACAAAATAGACAACGGGTTCTTATCATCTTTGGTATCAAACACAATACAAGGAATCAACAACATAGTTCTTTCAAAATCTTGCGCTTATGATCACCAGCACTTAACTCCAGATTTTCATGCTAATCTTGAAAAGAGCTGCCCCAATTCGGAACCCCAAAGGGCCCGAAATTGCGAGGCAATGGAGTCTCCCAATGCGGCGAATGTGATCACTGGCAAGAGTTTGTACACAGACTCACTGCTTCCTCACTCTCTTCGCAGGACAGTTTCTCTCCCAGTTGATGCTGTTGATTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007623 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

746

Amino Acids

85.13

Weight (kDa)

5.08

Isoelectric Point (pI)

43.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	27 - 281	8.1e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 267	1.6e-31	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001186)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04910 AT3G04910 AT3G04910 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080
fragaria_vesca	FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_6g03470 FvH4_6g03470
malus_domestica	MD04G1220500.v1.1 MD12G1236900.v1.1 MD13G1192500.v1.1 MD16G1193000.v1.1
prunus_persica	Prupe.1G013100_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1
pyrus_communis	pycom04g19520 pycom12g21800 pycom13g16490 pycom16g16300
rosa_chinensis	RchiOBHm_Chr3g0451971 RchiOBHm_Chr4g0416321
rosa_laevigata	RLG00000007995 RLG00000025633
rosa_multiflora	Rmu_sc0001145.1_g000025 Rmu_sc0006436.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436150 Rroxscaffold_5G00360170 Rroxscaffold_6G00429140
rosa_rugosa	Rorug02G0637900 Rorug04G0140700 Rorug04G0140700
rosa_samantha	Rh3AG039500 Rh3BG040900 Rh3CG039400 Rh3DG040100 Rh4AG202900 Rh4BG200300 Rh4CG214500 Rh4DG200200
rosa_wichuraiana	Rw3G002970 Rw4G017170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2236
AccB1I	GGYRCC	2 cut(s)	891, 1297
AccBSI	CCGCTC	1 cut(s)	551
AccI	GTMKAC	1 cut(s)	1077
AciI	CCGC	3 cut(s)	549, 1790, 2138
AclWI	GGATC	5 cut(s)	47, 439, 749, 1639, 1683
AcsI	RAATTY	6 cut(s)	260, 770, 1284, 1587, 1744, 1830
AcuI	CTGAAG	2 cut(s)	213, 606
AfaI	GTAC	6 cut(s)	743, 765, 1158, 1505, 1799, 2168
AfeI	AGCGCT	1 cut(s)	1429
AfiI	CCNNNNNNNGG	1 cut(s)	2089
AflII	CTTAAG	3 cut(s)	335, 458, 532
AjnI	CCWGG	3 cut(s)	159, 272, 1318
AjuI	GAANNNNNNNTTGG	2 cut(s)	489, 521
AleI	CACNNNNGTG	1 cut(s)	1368
Alw21I	GWGCWC	1 cut(s)	1050
Alw26I	GTCTC	3 cut(s)	14, 1120, 2133
AlwI	GGATC	5 cut(s)	47, 439, 749, 1639, 1683
Ama87I	CYCGRG	1 cut(s)	1636
Aor51HI	AGCGCT	1 cut(s)	1429
AoxI	GGCC	2 cut(s)	1558, 2103
ApaI	GGGCCC	1 cut(s)	2107
ApeKI	GCWGC	3 cut(s)	524, 922, 2079
ApoI	RAATTY	6 cut(s)	260, 770, 1284, 1587, 1744, 1830
ArsI	GACNNNNNNTTYG	2 cut(s)	1345, 1377
AspLEI	GCGC	2 cut(s)	1430, 2027
AspS9I	GGNCC	5 cut(s)	330, 908, 1316, 2103, 2104
AsuC2I	CCSGG	1 cut(s)	1339
AsuHPI	GGTGA	5 cut(s)	313, 521, 1231, 1553, 2027
AsuII	TTCGAA	2 cut(s)	663, 1132
AsuNHI	GCTAGC	1 cut(s)	1441
AvaI	CYCGRG	1 cut(s)	1636
AvaII	GGWCC	3 cut(s)	330, 908, 1316
BaeGI	GKGCMC	2 cut(s)	1302, 2107
BanI	GGYRCC	2 cut(s)	891, 1297
BanII	GRGCYC	3 cut(s)	191, 1790, 2107
BarI	GAAGNNNNNNTAC	2 cut(s)	1140, 1172
BbsI	GAAGAC	1 cut(s)	1113
Bbv12I	GWGCWC	1 cut(s)	1050
BbvCI	CCTCAGC	1 cut(s)	1877
BbvI	GCAGC	3 cut(s)	511, 934, 2066
BccI	CCATC	5 cut(s)	1271, 1396, 1757, 1842, 1864
BcgI	CGANNNNNNTGC	2 cut(s)	780, 814
BciT130I	CCWGG	3 cut(s)	161, 274, 1320
BciVI	GTATCC	1 cut(s)	962
BclI	TGATCA	3 cut(s)	1060, 2032, 2148
BcnI	CCSGG	1 cut(s)	1339
BcoDI	GTCTC	3 cut(s)	14, 1120, 2133
BfaI	CTAG	7 cut(s)	614, 878, 896, 1325, 1389, 1425, 1442
BfmI	CTRYAG	1 cut(s)	182
BfoI	RGCGCY	1 cut(s)	1431
BfrI	CTTAAG	3 cut(s)	335, 458, 532
BfuI	GTATCC	1 cut(s)	962
BglI	GCCNNNNNGGC	1 cut(s)	1306
BglII	AGATCT	4 cut(s)	196, 399, 454, 699
BisI	GCNGC	5 cut(s)	525, 549, 923, 2080, 2139
BlsI	GCNGC	5 cut(s)	526, 550, 924, 2081, 2140
Bme1390I	CCNGG	4 cut(s)	161, 274, 1320, 1339
Bme18I	GGWCC	3 cut(s)	330, 908, 1316
BmeT110I	CYCGRG	1 cut(s)	1636
BmgT120I	GGNCC	5 cut(s)	330, 908, 1316, 2103, 2104
BmrFI	CCNGG	4 cut(s)	161, 274, 1320, 1339
BmrI	ACTGGG	2 cut(s)	69, 2210
BmsI	GCATC	6 cut(s)	724, 982, 1125, 1534, 1739, 2212
BmtI	GCTAGC	1 cut(s)	1445
BmuI	ACTGGG	2 cut(s)	69, 2210
BoxI	GACNNNNGTC	1 cut(s)	906
BpiI	GAAGAC	1 cut(s)	1113
BpmI	CTGGAG	1 cut(s)	2034
Bpu10I	CCTNAGC	2 cut(s)	1431, 1877
Bpu14I	TTCGAA	2 cut(s)	663, 1132
BpuEI	CTTGAG	3 cut(s)	215, 221, 424
BpuMI	CCSGG	1 cut(s)	1339
BsaAI	YACGTR	1 cut(s)	1503
BsaI	GGTCTC	1 cut(s)	14
BsaJI	CCNNGG	5 cut(s)	88, 273, 490, 514, 1337
BsaXI	ACNNNNNCTCC	4 cut(s)	1397, 1427, 1487, 1517
Bsc4I	CCNNNNNNNGG	1 cut(s)	2089
Bse1I	ACTGG	4 cut(s)	64, 1033, 2158, 2216
Bse3DI	GCAATG	1 cut(s)	2127
BseBI	CCWGG	3 cut(s)	161, 274, 1320
BseDI	CCNNGG	5 cut(s)	88, 273, 490, 514, 1337
BseGI	GGATG	5 cut(s)	642, 688, 1063, 1255, 1730
BseLI	CCNNNNNNNGG	1 cut(s)	2089
BseMI	GCAATG	1 cut(s)	2127
BseMII	CTCAG	4 cut(s)	710, 1176, 1512, 1868
BseNI	ACTGG	4 cut(s)	64, 1033, 2158, 2216
BseRI	GAGGAG	3 cut(s)	218, 1116, 1508
BseSI	GKGCMC	2 cut(s)	1302, 2107
BseXI	GCAGC	3 cut(s)	511, 934, 2066
Bsh1285I	CGRYCG	2 cut(s)	835, 1486
BshFI	GGCC	2 cut(s)	1560, 2105
BshNI	GGYRCC	2 cut(s)	891, 1297
BsiEI	CGRYCG	2 cut(s)	835, 1486
BsiHKAI	GWGCWC	1 cut(s)	1050
BsiHKCI	CYCGRG	1 cut(s)	1636
BsiSI	CCGG	1 cut(s)	1339
BslFI	GGGAC	4 cut(s)	343, 577, 1608, 1653
BslI	CCNNNNNNNGG	1 cut(s)	2089
BsmAI	GTCTC	3 cut(s)	14, 1120, 2133
BsmFI	GGGAC	4 cut(s)	343, 577, 1608, 1653
BsmI	GAATGC	2 cut(s)	686, 1933
BsnI	GGCC	2 cut(s)	1560, 2105
Bso31I	GGTCTC	1 cut(s)	14
BsoBI	CYCGRG	1 cut(s)	1636
Bsp119I	TTCGAA	2 cut(s)	663, 1132
Bsp120I	GGGCCC	1 cut(s)	2103
Bsp1286I	GDGCHC	5 cut(s)	191, 1050, 1302, 1790, 2107
Bsp1407I	TGTACA	2 cut(s)	741, 2166
BspACI	CCGC	3 cut(s)	549, 1790, 2138
BspANI	GGCC	2 cut(s)	1560, 2105
BspCNI	CTCAG	4 cut(s)	709, 1177, 1513, 1869
BspHI	TCATGA	1 cut(s)	255
BspMAI	CTGCAG	1 cut(s)	186
BspOI	GCTAGC	1 cut(s)	1445
BspPI	GGATC	5 cut(s)	47, 439, 749, 1639, 1683
BspQI	GCTCTTC	1 cut(s)	1452
BspT104I	TTCGAA	2 cut(s)	663, 1132
BspT107I	GGYRCC	2 cut(s)	891, 1297
BspTI	CTTAAG	3 cut(s)	335, 458, 532
BspTNI	GGTCTC	1 cut(s)	14
BsrBI	CCGCTC	1 cut(s)	551
BsrDI	GCAATG	1 cut(s)	2127
BsrGI	TGTACA	2 cut(s)	741, 2166
BsrI	ACTGG	4 cut(s)	64, 1033, 2158, 2216
BssECI	CCNNGG	5 cut(s)	88, 273, 490, 514, 1337
BssT1I	CCWWGG	3 cut(s)	88, 490, 514
Bst2UI	CCWGG	3 cut(s)	161, 274, 1320
Bst6I	CTCTTC	4 cut(s)	591, 1091, 1452, 2199
BstAFI	CTTAAG	3 cut(s)	335, 458, 532
BstAUI	TGTACA	2 cut(s)	741, 2166
BstBAI	YACGTR	1 cut(s)	1503
BstBI	TTCGAA	2 cut(s)	663, 1132
BstC8I	GCNNGC	3 cut(s)	546, 1443, 1790
BstDEI	CTNAG	8 cut(s)	105, 696, 1185, 1431, 1521, 1622, 1681, 1877
BstEII	GGTNACC	1 cut(s)	509
BstF5I	GGATG	5 cut(s)	642, 688, 1063, 1255, 1730
BstH2I	RGCGCY	1 cut(s)	1431
BstHHI	GCGC	2 cut(s)	1430, 2027
BstMAI	GTCTC	3 cut(s)	14, 1120, 2133
BstMCI	CGRYCG	2 cut(s)	835, 1486
BstMWI	GCNNNNNNNGC	2 cut(s)	1306, 1882
BstNI	CCWGG	3 cut(s)	161, 274, 1320
BstPAI	GACNNNNGTC	1 cut(s)	906
BstPI	GGTNACC	1 cut(s)	509
BstSCI	CCNGG	4 cut(s)	159, 272, 1318, 1337
BstSFI	CTRYAG	1 cut(s)	182
BstSLI	GKGCMC	2 cut(s)	1302, 2107
BstSNI	TACGTA	1 cut(s)	1503
BstV1I	GCAGC	3 cut(s)	511, 934, 2066
BstV2I	GAAGAC	1 cut(s)	1113
BstX2I	RGATCY	6 cut(s)	196, 399, 454, 699, 754, 1644
BstXI	CCANNNNNNTGG	1 cut(s)	1005
BstYI	RGATCY	6 cut(s)	196, 399, 454, 699, 754, 1644
BsuI	GTATCC	1 cut(s)	962
BsuRI	GGCC	2 cut(s)	1560, 2105
BtsCI	GGATG	5 cut(s)	642, 688, 1063, 1255, 1730
BtsI	GCAGTG	3 cut(s)	384, 553, 2177
BtsIMutI	CAGTG	6 cut(s)	309, 384, 553, 1182, 2151, 2177
Cac8I	GCNNGC	3 cut(s)	546, 1443, 1790
CciI	TCATGA	1 cut(s)	255
CfoI	GCGC	2 cut(s)	1430, 2027
Cfr13I	GGNCC	5 cut(s)	330, 908, 1316, 2103, 2104
CseI	GACGC	1 cut(s)	786
CsiI	ACCWGGT	1 cut(s)	159
Csp6I	GTAC	6 cut(s)	742, 764, 1157, 1504, 1798, 2167
CviQI	GTAC	6 cut(s)	742, 764, 1157, 1504, 1798, 2167
DdeI	CTNAG	8 cut(s)	105, 696, 1185, 1431, 1521, 1622, 1681, 1877
DraI	TTTAAA	1 cut(s)	828
Eam1104I	CTCTTC	4 cut(s)	591, 1091, 1452, 2199
EarI	CTCTTC	4 cut(s)	591, 1091, 1452, 2199
Eco105I	TACGTA	1 cut(s)	1503
Eco130I	CCWWGG	3 cut(s)	88, 490, 514
Eco24I	GRGCYC	3 cut(s)	191, 1790, 2107
Eco31I	GGTCTC	1 cut(s)	14
Eco32I	GATATC	1 cut(s)	1237
Eco47I	GGWCC	3 cut(s)	330, 908, 1316
Eco47III	AGCGCT	1 cut(s)	1429
Eco57I	CTGAAG	2 cut(s)	213, 606
Eco88I	CYCGRG	1 cut(s)	1636
Eco91I	GGTNACC	1 cut(s)	509
EcoO109I	RGGNCCY	3 cut(s)	330, 908, 2103
EcoO65I	GGTNACC	1 cut(s)	509
EcoRI	GAATTC	1 cut(s)	1830
EcoRII	CCWGG	3 cut(s)	159, 272, 1318
EcoRV	GATATC	1 cut(s)	1237
EcoT14I	CCWWGG	3 cut(s)	88, 490, 514
EcoT38I	GRGCYC	3 cut(s)	191, 1790, 2107
ErhI	CCWWGG	3 cut(s)	88, 490, 514
FaqI	GGGAC	4 cut(s)	343, 577, 1608, 1653
FauI	CCCGC	1 cut(s)	1797
FbaI	TGATCA	3 cut(s)	1060, 2032, 2148
FblI	GTMKAC	1 cut(s)	1077
Fnu4HI	GCNGC	5 cut(s)	525, 549, 923, 2080, 2139
FokI	GGATG	5 cut(s)	649, 675, 1070, 1262, 1717
FriOI	GRGCYC	3 cut(s)	191, 1790, 2107
Fsp4HI	GCNGC	5 cut(s)	525, 549, 923, 2080, 2139
FspBI	CTAG	7 cut(s)	614, 878, 896, 1325, 1389, 1425, 1442
GlaI	GCGC	2 cut(s)	1429, 2026
GluI	GCNGC	5 cut(s)	525, 549, 923, 2080, 2139
GsuI	CTGGAG	1 cut(s)	2034
HaeII	RGCGCY	1 cut(s)	1431
HaeIII	GGCC	2 cut(s)	1560, 2105
HapII	CCGG	1 cut(s)	1339
HgaI	GACGC	1 cut(s)	786
HhaI	GCGC	2 cut(s)	1430, 2027
Hin6I	GCGC	2 cut(s)	1428, 2025
HinP1I	GCGC	2 cut(s)	1428, 2025
HincII	GTYRAC	2 cut(s)	619, 1078
HindII	GTYRAC	2 cut(s)	619, 1078
HindIII	AAGCTT	2 cut(s)	167, 1354
HinfI	GANTC	9 cut(s)	1022, 1032, 1517, 1553, 1706, 1905, 1995, 2126, 2174
HpaII	CCGG	1 cut(s)	1339
HphI	GGTGA	5 cut(s)	313, 521, 1231, 1553, 2027
Hpy188I	TCNGA	9 cut(s)	699, 1069, 1522, 1567, 1654, 1735, 1754, 1836, 2092
Hpy99I	CGWCG	1 cut(s)	802
HpyAV	CCTTC	4 cut(s)	131, 1142, 1482, 1831
HpyCH4IV	ACGT	1 cut(s)	1502
HpyCH4V	TGCA	8 cut(s)	184, 527, 684, 917, 1226, 1480, 1782, 1931
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1306, 1882
HpyF3I	CTNAG	8 cut(s)	105, 696, 1185, 1431, 1521, 1622, 1681, 1877
HpySE526I	ACGT	1 cut(s)	1502
HspAI	GCGC	2 cut(s)	1428, 2025
KflI	GGGWCCC	1 cut(s)	330
Ksp22I	TGATCA	3 cut(s)	1060, 2032, 2148
LguI	GCTCTTC	1 cut(s)	1452
LmnI	GCTCC	5 cut(s)	98, 586, 820, 1873, 1882
Lsp1109I	GCAGC	3 cut(s)	511, 934, 2066
LweI	GCATC	6 cut(s)	724, 982, 1125, 1534, 1739, 2212
MabI	ACCWGGT	1 cut(s)	159
MaeI	CTAG	7 cut(s)	614, 878, 896, 1325, 1389, 1425, 1442
MaeII	ACGT	1 cut(s)	1502
MaeIII	GTNAC	8 cut(s)	307, 464, 509, 655, 1361, 1531, 1684, 1817
MbiI	CCGCTC	1 cut(s)	551
MfeI	CAATTG	1 cut(s)	1366
MflI	RGATCY	6 cut(s)	196, 399, 454, 699, 754, 1644
MhlI	GDGCHC	5 cut(s)	191, 1050, 1302, 1790, 2107
MlyI	GAGTC	3 cut(s)	1526, 2135, 2168
MmeI	TCCRAC	2 cut(s)	80, 1212
MseI	TTAA	8 cut(s)	336, 350, 459, 533, 750, 827, 1089, 2045
MslI	CAYNNNNRTG	7 cut(s)	314, 429, 897, 1056, 1068, 1295, 1368
MspCI	CTTAAG	3 cut(s)	335, 458, 532
MspI	CCGG	1 cut(s)	1339
MspR9I	CCNGG	4 cut(s)	161, 274, 1320, 1339
MunI	CAATTG	1 cut(s)	1366
Mva1269I	GAATGC	2 cut(s)	686, 1933
MvaI	CCWGG	3 cut(s)	161, 274, 1320
MwoI	GCNNNNNNNGC	2 cut(s)	1306, 1882
NciI	CCSGG	1 cut(s)	1339
NheI	GCTAGC	1 cut(s)	1441
NmuCI	GTSAC	7 cut(s)	307, 464, 509, 655, 1361, 1531, 1684
NspV	TTCGAA	2 cut(s)	663, 1132
OliI	CACNNNNGTG	1 cut(s)	1368
PagI	TCATGA	1 cut(s)	255
PciSI	GCTCTTC	1 cut(s)	1452
PctI	GAATGC	2 cut(s)	686, 1933
PfeI	GAWTC	6 cut(s)	1022, 1032, 1553, 1706, 1905, 1995
PkrI	GCNGC	5 cut(s)	526, 550, 924, 2081, 2140
PleI	GAGTC	3 cut(s)	1525, 2134, 2168
PpsI	GAGTC	3 cut(s)	1525, 2134, 2168
Ppu21I	YACGTR	1 cut(s)	1503
PpuMI	RGGWCCY	2 cut(s)	330, 908
PshAI	GACNNNNGTC	1 cut(s)	906
PsiI	TTATAA	1 cut(s)	2236
Psp5II	RGGWCCY	2 cut(s)	330, 908
Psp6I	CCWGG	3 cut(s)	159, 272, 1318
PspEI	GGTNACC	1 cut(s)	509
PspGI	CCWGG	3 cut(s)	159, 272, 1318
PspOMI	GGGCCC	1 cut(s)	2103
PspPI	GGNCC	5 cut(s)	330, 908, 1316, 2103, 2104
PspPPI	RGGWCCY	2 cut(s)	330, 908
PstI	CTGCAG	1 cut(s)	186
PsuI	RGATCY	6 cut(s)	196, 399, 454, 699, 754, 1644
RsaI	GTAC	6 cut(s)	743, 765, 1158, 1505, 1799, 2168
RsaNI	GTAC	6 cut(s)	742, 764, 1157, 1504, 1798, 2167
RseI	CAYNNNNRTG	7 cut(s)	314, 429, 897, 1056, 1068, 1295, 1368
SalI	GTCGAC	1 cut(s)	1076
SapI	GCTCTTC	1 cut(s)	1452
SaqAI	TTAA	8 cut(s)	336, 350, 459, 533, 750, 827, 1089, 2045
SatI	GCNGC	5 cut(s)	525, 549, 923, 2080, 2139
Sau96I	GGNCC	5 cut(s)	330, 908, 1316, 2103, 2104
SchI	GAGTC	3 cut(s)	1526, 2135, 2168
ScrFI	CCNGG	4 cut(s)	161, 274, 1320, 1339
SduI	GDGCHC	5 cut(s)	191, 1050, 1302, 1790, 2107
SexAI	ACCWGGT	1 cut(s)	159
SfaNI	GCATC	6 cut(s)	724, 982, 1125, 1534, 1739, 2212
SfcI	CTRYAG	1 cut(s)	182
SfuI	TTCGAA	2 cut(s)	663, 1132
SinI	GGWCC	3 cut(s)	330, 908, 1316
SmiMI	CAYNNNNRTG	7 cut(s)	314, 429, 897, 1056, 1068, 1295, 1368
SmlI	CTYRAG	6 cut(s)	200, 230, 335, 403, 458, 532
SmoI	CTYRAG	6 cut(s)	200, 230, 335, 403, 458, 532
SnaBI	TACGTA	1 cut(s)	1503
SsiI	CCGC	3 cut(s)	549, 1790, 2138
SspMI	CTAG	7 cut(s)	614, 878, 896, 1325, 1389, 1425, 1442
StyD4I	CCNGG	4 cut(s)	159, 272, 1318, 1337
StyI	CCWWGG	3 cut(s)	88, 490, 514
TaiI	ACGT	1 cut(s)	1505
TaqI	TCGA	8 cut(s)	663, 943, 974, 1035, 1077, 1132, 1415, 1888
TatI	WGTACW	2 cut(s)	741, 2166
TauI	GCSGC	2 cut(s)	551, 2141
TfiI	GAWTC	6 cut(s)	1022, 1032, 1553, 1706, 1905, 1995
Tru1I	TTAA	8 cut(s)	336, 350, 459, 533, 750, 827, 1089, 2045
Tru9I	TTAA	8 cut(s)	336, 350, 459, 533, 750, 827, 1089, 2045
TscAI	CASTG	6 cut(s)	316, 384, 560, 1189, 2158, 2184
TseFI	GTSAC	7 cut(s)	307, 464, 509, 655, 1361, 1531, 1684
TseI	GCWGC	3 cut(s)	524, 922, 2079
Tsp45I	GTSAC	7 cut(s)	307, 464, 509, 655, 1361, 1531, 1684
TspGWI	ACGGA	1 cut(s)	660
TspRI	CASTG	6 cut(s)	316, 384, 560, 1189, 2158, 2184
Vha464I	CTTAAG	3 cut(s)	335, 458, 532
VpaK11BI	GGWCC	3 cut(s)	330, 908, 1316
XapI	RAATTY	6 cut(s)	260, 770, 1284, 1587, 1744, 1830
XmiI	GTMKAC	1 cut(s)	1077
XspI	CTAG	7 cut(s)	614, 878, 896, 1325, 1389, 1425, 1442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.