Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0001186

Physical Location & Seq

Genomic Coordinates Forward (+)

12240237 .. 12241656

Gene Structure 1420 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g16300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGTATGAAATAGGGCCTCTCTTAAGGCAACCTCACTCTGCACTTCACCAAAGTAACAGCTCTTTGAGCAACGGGTATGGAAATTATCTTGGTTACGAAACTGAAAATGATTTGGATTACAATCCGGTTGATTACAGATTGAGCGAAATTGATCTCTTAGCTTGTCAAGAGGACGAGCATTTGGGAGATGTTGACCTCACAATCAAAGGGAGGCGGAGAGAAGACGATGGCATCTTTTTAAGGCTCAGAATTGCAGATAAAGAAGGCCGCATCCGAAACATCTACTTCCCTTTTGATACTGAGAATGACACGGCATTGAGTGTTGCTAGAGAGATGGTTTCTGAGCTTGATATCACAGACCAAGATGTTACAAAAATAGCAGAGATGATTGATGGTGAAATTGCTAGCTTGGTACCGGAATGGATTGGGGGACATGGCATGGAAGAAATTCCCCATTGGAATAATGCAAGTTTTTGCCAAAATTGTGCTTCATATGGTTCCATTTTGGATTATGCATCATCAGATATTCCAGGCGTCAATCACCTCCAAGTTCTTCAGTGCTCAAAACACGGATCAGCTGCTGTCCATGGACGTTTCGAAGAAATCACCTACCAAGTTGAAGGCTCAGAGCAAAGTCTTACAGAAGGTGCACCTAGAACATCAAACCATTCTGATGGCTTGCACTATGCCGATATCTGGGCTCAAAGAGAAGGACCGGAATTAAGCTCACAAGGTTCAGGAGATTCGAGAGACACCCAATGCGATGAAAATCATGAAACATTGGATGCAACAAACTTTGGAAAGGAAGAGAGAATCATAACCATGGACAATCAGAGCAGCTCGAAAGCAAGAAATTCTATCTCAGAGAAACTTTCAGCCACTAATGATCCGTTAGATGATTATGAGAATGAGCTTCGTCAAGAACTAAGATGGCTCAAAGCAACGTATCAAATGCAGTTGAGGGAGATTAGGGATCAACAGCTTGGAGCTAAACCGAAAGCTTCAAGCCTAAGTCCAAATTCAGACCACTCAGAACACAGGGAAACAAATGGAGCTTCAATCTCCTTGGCATCGCCCCAGCGGACCAAAGAAAACAAAAAACCTTCCTTGAAACCGTTTTGCTCCGGGAAGCATTTTACATATTTTCCAGTGGATGCCGAAAAGAAACATTTAGCAAACCCTGGTGCCCAAAATCATGAGGCATTTGATGGGGCTTATAGTCCTGAACAAATCGTCACTGCAAAGAGTTTCTACGCTAGTGCGTTGCTTCCGCACTCTCTTCACCGTGCAACTTCTCTACCGGTTGATGCTACAGATGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048511 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

441

Amino Acids

49.12

Weight (kDa)

4.89

Isoelectric Point (pI)

42.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
OSR1_C	PF12202	79 - 134	3.8e-06	Oxidative-stress-responsive kinase 1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001186)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04910 AT3G04910 AT3G04910 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080
fragaria_vesca	FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_6g03470 FvH4_6g03470
malus_domestica	MD04G1220500.v1.1 MD12G1236900.v1.1 MD13G1192500.v1.1 MD16G1193000.v1.1
prunus_persica	Prupe.1G013100_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1
pyrus_communis	pycom04g19520 pycom12g21800 pycom13g16490 pycom16g16300
rosa_chinensis	RchiOBHm_Chr3g0451971 RchiOBHm_Chr4g0416321
rosa_laevigata	RLG00000007995 RLG00000025633
rosa_multiflora	Rmu_sc0001145.1_g000025 Rmu_sc0006436.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436150 Rroxscaffold_5G00360170 Rroxscaffold_6G00429140
rosa_rugosa	Rorug02G0637900 Rorug04G0140700 Rorug04G0140700
rosa_samantha	Rh3AG039500 Rh3BG040900 Rh3CG039400 Rh3DG040100 Rh4AG202900 Rh4BG200300 Rh4CG214500 Rh4DG200200
rosa_wichuraiana	Rw3G002970 Rw4G017170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	412
AccB1I	GGYRCC	2 cut(s)	412, 1184
AciI	CCGC	4 cut(s)	214, 268, 1081, 1271
AclWI	GGATC	3 cut(s)	580, 881, 981
AcsI	RAATTY	3 cut(s)	447, 853, 1018
AcuI	CTGAAG	1 cut(s)	539
AcyI	GRCGYC	1 cut(s)	534
AfaI	GTAC	1 cut(s)	414
AfiI	CCNNNNNNNGG	1 cut(s)	696
AflII	CTTAAG	1 cut(s)	22
AgeI	ACCGGT	1 cut(s)	1300
AgsI	TTSAA	4 cut(s)	620, 1005, 1059, 1111
AjnI	CCWGG	2 cut(s)	529, 1180
Alw21I	GWGCWC	2 cut(s)	563, 652
Alw26I	GTCTC	1 cut(s)	744
Alw44I	GTGCAC	1 cut(s)	648
AlwI	GGATC	3 cut(s)	580, 881, 981
AlwNI	CAGNNNCTG	1 cut(s)	581
AoxI	GGCC	2 cut(s)	14, 265
ApaLI	GTGCAC	1 cut(s)	648
ApeKI	GCWGC	2 cut(s)	578, 837
ApoI	RAATTY	3 cut(s)	447, 853, 1018
AsiGI	ACCGGT	1 cut(s)	1300
Asp700I	GAANNNNTTC	1 cut(s)	447
Asp718I	GGTACC	1 cut(s)	412
AspS9I	GGNCC	3 cut(s)	14, 713, 1083
AsuC2I	CCSGG	1 cut(s)	1126
AsuHPI	GGTGA	5 cut(s)	38, 407, 533, 598, 1274
AsuII	TTCGAA	1 cut(s)	597
AsuNHI	GCTAGC	1 cut(s)	404
AvaII	GGWCC	2 cut(s)	713, 1083
BaeGI	GKGCMC	2 cut(s)	652, 1189
BanI	GGYRCC	2 cut(s)	412, 1184
BanII	GRGCYC	1 cut(s)	703
BbsI	GAAGAC	1 cut(s)	228
Bbv12I	GWGCWC	2 cut(s)	563, 652
BbvI	GCAGC	2 cut(s)	565, 849
BccI	CCATC	6 cut(s)	221, 328, 386, 668, 924, 1202
BceAI	ACGGC	1 cut(s)	327
BciT130I	CCWGG	2 cut(s)	531, 1182
BcnI	CCSGG	1 cut(s)	1126
BcoDI	GTCTC	1 cut(s)	744
BfaI	CTAG	4 cut(s)	327, 405, 654, 1257
BfmI	CTRYAG	1 cut(s)	1311
BfrI	CTTAAG	1 cut(s)	22
BisI	GCNGC	3 cut(s)	268, 579, 838
BlsI	GCNGC	3 cut(s)	269, 580, 839
Bme1390I	CCNGG	3 cut(s)	531, 1126, 1182
Bme18I	GGWCC	2 cut(s)	713, 1083
BmgT120I	GGNCC	3 cut(s)	14, 713, 1083
BmiI	GGNNCC	3 cut(s)	414, 499, 1186
BmrFI	CCNGG	3 cut(s)	531, 1126, 1182
BmsI	GCATC	7 cut(s)	240, 279, 524, 775, 1079, 1144, 1297
BmtI	GCTAGC	1 cut(s)	408
BpiI	GAAGAC	1 cut(s)	228
Bpu14I	TTCGAA	1 cut(s)	597
BpuMI	CCSGG	1 cut(s)	1126
BsaBI	GATNNNNATC	2 cut(s)	120, 768
BsaHI	GRCGYC	1 cut(s)	534
BsaJI	CCNNGG	4 cut(s)	586, 822, 1065, 1180
BsaWI	WCCGGW	4 cut(s)	124, 415, 715, 1300
Bsc4I	CCNNNNNNNGG	1 cut(s)	696
Bse118I	RCCGGY	1 cut(s)	1300
Bse1I	ACTGG	1 cut(s)	1148
Bse8I	GATNNNNATC	2 cut(s)	120, 768
BseBI	CCWGG	2 cut(s)	531, 1182
BseDI	CCNNGG	4 cut(s)	586, 822, 1065, 1180
BseGI	GGATG	3 cut(s)	270, 790, 1159
BseJI	GATNNNNATC	2 cut(s)	120, 768
BseLI	CCNNNNNNNGG	1 cut(s)	696
BseMII	CTCAG	6 cut(s)	259, 291, 333, 639, 876, 1044
BseNI	ACTGG	1 cut(s)	1148
BseSI	GKGCMC	2 cut(s)	652, 1189
BseXI	GCAGC	2 cut(s)	565, 849
BseYI	CCCAGC	1 cut(s)	1077
BsgI	GTGCAG	1 cut(s)	24
BshFI	GGCC	2 cut(s)	16, 267
BshNI	GGYRCC	2 cut(s)	412, 1184
BshTI	ACCGGT	1 cut(s)	1300
BsiHKAI	GWGCWC	2 cut(s)	563, 652
BsiSI	CCGG	5 cut(s)	125, 416, 716, 1125, 1301
BslFI	GGGAC	1 cut(s)	444
BslI	CCNNNNNNNGG	1 cut(s)	696
BsmAI	GTCTC	1 cut(s)	744
BsmFI	GGGAC	1 cut(s)	444
BsnI	GGCC	2 cut(s)	16, 267
Bsp119I	TTCGAA	1 cut(s)	597
Bsp1286I	GDGCHC	4 cut(s)	563, 652, 703, 1189
Bsp143I	GATC	4 cut(s)	151, 572, 886, 973
Bsp19I	CCATGG	2 cut(s)	586, 822
BspACI	CCGC	4 cut(s)	214, 268, 1081, 1271
BspANI	GGCC	2 cut(s)	16, 267
BspCNI	CTCAG	6 cut(s)	258, 292, 334, 638, 875, 1043
BspHI	TCATGA	2 cut(s)	772, 1195
BspLI	GGNNCC	3 cut(s)	414, 499, 1186
BspOI	GCTAGC	1 cut(s)	408
BspPI	GGATC	3 cut(s)	580, 881, 981
BspT104I	TTCGAA	1 cut(s)	597
BspT107I	GGYRCC	2 cut(s)	412, 1184
BspTI	CTTAAG	1 cut(s)	22
BsrFI	RCCGGY	1 cut(s)	1300
BsrI	ACTGG	1 cut(s)	1148
BssAI	RCCGGY	1 cut(s)	1300
BssECI	CCNNGG	4 cut(s)	586, 822, 1065, 1180
BssMI	GATC	4 cut(s)	151, 572, 886, 973
BssNI	GRCGYC	1 cut(s)	534
BssT1I	CCWWGG	3 cut(s)	586, 822, 1065
Bst2UI	CCWGG	2 cut(s)	531, 1182
Bst4CI	ACNGT	2 cut(s)	1116, 1286
Bst6I	CTCTTC	2 cut(s)	801, 1284
BstACI	GRCGYC	1 cut(s)	534
BstAFI	CTTAAG	1 cut(s)	22
BstBI	TTCGAA	1 cut(s)	597
BstC8I	GCNNGC	2 cut(s)	406, 680
BstDEI	CTNAG	9 cut(s)	157, 245, 300, 342, 625, 862, 926, 1010, 1030
BstDSI	CCRYGG	2 cut(s)	586, 822
BstF5I	GGATG	3 cut(s)	270, 790, 1159
BstKTI	GATC	4 cut(s)	154, 575, 889, 976
BstMAI	GTCTC	1 cut(s)	744
BstMBI	GATC	4 cut(s)	151, 572, 886, 973
BstMWI	GCNNNNNNNGC	1 cut(s)	66
BstNI	CCWGG	2 cut(s)	531, 1182
BstSCI	CCNGG	3 cut(s)	529, 1124, 1180
BstSFI	CTRYAG	1 cut(s)	1311
BstSLI	GKGCMC	2 cut(s)	652, 1189
BstV1I	GCAGC	2 cut(s)	565, 849
BstV2I	GAAGAC	1 cut(s)	228
BstXI	CCANNNNNNTGG	1 cut(s)	674
BsuRI	GGCC	2 cut(s)	16, 267
BtgI	CCRYGG	2 cut(s)	586, 822
BtgZI	GCGATG	2 cut(s)	777, 1056
BtsCI	GGATG	3 cut(s)	270, 790, 1159
BtsI	GCAGTG	1 cut(s)	1236
BtsIMutI	CAGTG	3 cut(s)	563, 1155, 1236
Cac8I	GCNNGC	2 cut(s)	406, 680
CaiI	CAGNNNCTG	1 cut(s)	581
CciI	TCATGA	2 cut(s)	772, 1195
Cfr10I	RCCGGY	1 cut(s)	1300
Cfr13I	GGNCC	3 cut(s)	14, 713, 1083
CseI	GACGC	1 cut(s)	523
Csp6I	GTAC	1 cut(s)	413
CspAI	ACCGGT	1 cut(s)	1300
CviAII	CATG	6 cut(s)	434, 439, 587, 773, 823, 1196
CviQI	GTAC	1 cut(s)	413
DdeI	CTNAG	9 cut(s)	157, 245, 300, 342, 625, 862, 926, 1010, 1030
DpnI	GATC	4 cut(s)	153, 574, 888, 975
DpnII	GATC	4 cut(s)	151, 572, 886, 973
Eam1104I	CTCTTC	2 cut(s)	801, 1284
EarI	CTCTTC	2 cut(s)	801, 1284
EciI	GGCGGA	1 cut(s)	229
Eco130I	CCWWGG	3 cut(s)	586, 822, 1065
Eco24I	GRGCYC	1 cut(s)	703
Eco32I	GATATC	2 cut(s)	352, 694
Eco47I	GGWCC	2 cut(s)	713, 1083
Eco57I	CTGAAG	1 cut(s)	539
EcoO109I	RGGNCCY	1 cut(s)	14
EcoRII	CCWGG	2 cut(s)	529, 1180
EcoRV	GATATC	2 cut(s)	352, 694
EcoT14I	CCWWGG	3 cut(s)	586, 822, 1065
EcoT22I	ATGCAT	1 cut(s)	517
EcoT38I	GRGCYC	1 cut(s)	703
ErhI	CCWWGG	3 cut(s)	586, 822, 1065
FaeI	CATG	6 cut(s)	437, 442, 590, 776, 826, 1199
FaqI	GGGAC	1 cut(s)	444
FatI	CATG	6 cut(s)	433, 438, 586, 772, 822, 1195
FauNDI	CATATG	1 cut(s)	493
Fnu4HI	GCNGC	3 cut(s)	268, 579, 838
FokI	GGATG	3 cut(s)	257, 797, 1166
FriOI	GRGCYC	1 cut(s)	703
Fsp4HI	GCNGC	3 cut(s)	268, 579, 838
FspBI	CTAG	4 cut(s)	327, 405, 654, 1257
GluI	GCNGC	3 cut(s)	268, 579, 838
GsaI	CCCAGC	1 cut(s)	1081
HaeIII	GGCC	2 cut(s)	16, 267
HapII	CCGG	5 cut(s)	125, 416, 716, 1125, 1301
HgaI	GACGC	1 cut(s)	523
Hin1I	GRCGYC	1 cut(s)	534
Hin1II	CATG	6 cut(s)	437, 442, 590, 776, 826, 1199
HincII	GTYRAC	1 cut(s)	193
HindII	GTYRAC	1 cut(s)	193
HindIII	AAGCTT	1 cut(s)	999
HinfI	GANTC	2 cut(s)	743, 813
HpaII	CCGG	5 cut(s)	125, 416, 716, 1125, 1301
HphI	GGTGA	5 cut(s)	38, 407, 533, 598, 1274
Hpy166II	GTNNAC	2 cut(s)	193, 650
Hpy188III	TCNNGA	7 cut(s)	167, 738, 747, 773, 920, 1196, 1223
Hpy8I	GTNNAC	2 cut(s)	193, 650
HpyAV	CCTTC	5 cut(s)	257, 614, 638, 704, 1113
HpyCH4III	ACNGT	2 cut(s)	1116, 1286
HpyCH4IV	ACGT	2 cut(s)	592, 944
HpyF10VI	GCNNNNNNNGC	1 cut(s)	66
HpyF3I	CTNAG	9 cut(s)	157, 245, 300, 342, 625, 862, 926, 1010, 1030
HpySE526I	ACGT	2 cut(s)	592, 944
Hsp92I	GRCGYC	1 cut(s)	534
Hsp92II	CATG	6 cut(s)	437, 442, 590, 776, 826, 1199
KpnI	GGTACC	1 cut(s)	416
Kzo9I	GATC	4 cut(s)	151, 572, 886, 973
LmnI	GCTCC	3 cut(s)	986, 1052, 1127
Lsp1109I	GCAGC	2 cut(s)	565, 849
LweI	GCATC	7 cut(s)	240, 279, 524, 775, 1079, 1144, 1297
MaeI	CTAG	4 cut(s)	327, 405, 654, 1257
MaeII	ACGT	2 cut(s)	592, 944
MaeIII	GTNAC	4 cut(s)	53, 92, 367, 1234
MalI	GATC	4 cut(s)	153, 574, 888, 975
MboI	GATC	4 cut(s)	151, 572, 886, 973
MboII	GAAGA	6 cut(s)	233, 455, 545, 611, 818, 1271
MhlI	GDGCHC	4 cut(s)	563, 652, 703, 1189
MluCI	AATT	9 cut(s)	82, 147, 249, 399, 447, 481, 719, 853, 1018
MnlI	CCTC	8 cut(s)	27, 42, 163, 204, 206, 554, 954, 1192
Mph1103I	ATGCAT	1 cut(s)	517
MroXI	GAANNNNTTC	1 cut(s)	447
MseI	TTAA	4 cut(s)	23, 239, 722, 1321
MslI	CAYNNNNRTG	2 cut(s)	672, 821
MspA1I	CMGCKG	2 cut(s)	578, 1081
MspCI	CTTAAG	1 cut(s)	22
MspI	CCGG	5 cut(s)	125, 416, 716, 1125, 1301
MspR9I	CCNGG	3 cut(s)	531, 1126, 1182
MvaI	CCWGG	2 cut(s)	531, 1182
MwoI	GCNNNNNNNGC	1 cut(s)	66
NciI	CCSGG	1 cut(s)	1126
NcoI	CCATGG	2 cut(s)	586, 822
NdeI	CATATG	1 cut(s)	493
NdeII	GATC	4 cut(s)	151, 572, 886, 973
NheI	GCTAGC	1 cut(s)	404
NlaIII	CATG	6 cut(s)	437, 442, 590, 776, 826, 1199
NlaIV	GGNNCC	3 cut(s)	414, 499, 1186
NmuCI	GTSAC	1 cut(s)	1234
NsiI	ATGCAT	1 cut(s)	517
NspV	TTCGAA	1 cut(s)	597
PagI	TCATGA	2 cut(s)	772, 1195
PdmI	GAANNNNTTC	1 cut(s)	447
PfeI	GAWTC	2 cut(s)	743, 813
PfoI	TCCNGGA	1 cut(s)	1124
PinAI	ACCGGT	1 cut(s)	1300
PkrI	GCNGC	3 cut(s)	269, 580, 839
Psp6I	CCWGG	2 cut(s)	529, 1180
PspFI	CCCAGC	1 cut(s)	1077
PspGI	CCWGG	2 cut(s)	529, 1180
PspN4I	GGNNCC	3 cut(s)	414, 499, 1186
PspPI	GGNCC	3 cut(s)	14, 713, 1083
PstNI	CAGNNNCTG	1 cut(s)	581
PvuII	CAGCTG	1 cut(s)	578
RsaI	GTAC	1 cut(s)	414
RsaNI	GTAC	1 cut(s)	413
RseI	CAYNNNNRTG	2 cut(s)	672, 821
SaqAI	TTAA	4 cut(s)	23, 239, 722, 1321
SatI	GCNGC	3 cut(s)	268, 579, 838
Sau3AI	GATC	4 cut(s)	151, 572, 886, 973
Sau96I	GGNCC	3 cut(s)	14, 713, 1083
ScrFI	CCNGG	3 cut(s)	531, 1126, 1182
SduI	GDGCHC	4 cut(s)	563, 652, 703, 1189
SfaNI	GCATC	7 cut(s)	240, 279, 524, 775, 1079, 1144, 1297
SfcI	CTRYAG	1 cut(s)	1311
SfuI	TTCGAA	1 cut(s)	597
SinI	GGWCC	2 cut(s)	713, 1083
SmiMI	CAYNNNNRTG	2 cut(s)	672, 821
SmlI	CTYRAG	1 cut(s)	22
SmoI	CTYRAG	1 cut(s)	22
Sse9I	AATT	9 cut(s)	82, 147, 249, 399, 447, 481, 719, 853, 1018
SsiI	CCGC	4 cut(s)	214, 268, 1081, 1271
SspMI	CTAG	4 cut(s)	327, 405, 654, 1257
StyD4I	CCNGG	3 cut(s)	529, 1124, 1180
StyI	CCWWGG	3 cut(s)	586, 822, 1065
TaaI	ACNGT	2 cut(s)	1116, 1286
TaiI	ACGT	2 cut(s)	595, 947
TaqI	TCGA	3 cut(s)	597, 746, 842
TasI	AATT	9 cut(s)	82, 147, 249, 399, 447, 481, 719, 853, 1018
TauI	GCSGC	1 cut(s)	270
TfiI	GAWTC	2 cut(s)	743, 813
Tru1I	TTAA	4 cut(s)	23, 239, 722, 1321
Tru9I	TTAA	4 cut(s)	23, 239, 722, 1321
TscAI	CASTG	3 cut(s)	563, 1155, 1243
TseFI	GTSAC	1 cut(s)	1234
TseI	GCWGC	2 cut(s)	578, 837
Tsp45I	GTSAC	1 cut(s)	1234
TspDTI	ATGAA	4 cut(s)	21, 480, 780, 789
TspGWI	ACGGA	2 cut(s)	585, 879
TspRI	CASTG	3 cut(s)	563, 1155, 1243
Vha464I	CTTAAG	1 cut(s)	22
VneI	GTGCAC	1 cut(s)	648
VpaK11BI	GGWCC	2 cut(s)	713, 1083
XapI	RAATTY	3 cut(s)	447, 853, 1018
XmnI	GAANNNNTTC	1 cut(s)	447
XspI	CTAG	4 cut(s)	327, 405, 654, 1257
Zsp2I	ATGCAT	1 cut(s)	517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.