Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1700 |
| AccII | CGCG | 1 cut(s) | 216 |
| AccIII | TCCGGA | 1 cut(s) | 2067 |
| AciI | CCGC | 3 cut(s) | 214, 1159, 1957 |
| AclWI | GGATC | 8 cut(s) | 47, 462, 749, 900, 1471, 1522, 1866, 1936 |
| AcoI | YGGCCR | 1 cut(s) | 1480 |
| AcsI | RAATTY | 6 cut(s) | 84, 219, 399, 572, 1735, 2056 |
| AcuI | CTGAAG | 3 cut(s) | 213, 827, 1430 |
| AfaI | GTAC | 4 cut(s) | 331, 1305, 1351, 1577 |
| AfeI | AGCGCT | 1 cut(s) | 738 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 932, 1535, 1891 |
| AjnI | CCWGG | 2 cut(s) | 1324, 1420 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 489, 521 |
| Alw26I | GTCTC | 2 cut(s) | 1652, 2160 |
| AlwI | GGATC | 8 cut(s) | 47, 462, 749, 900, 1471, 1522, 1866, 1936 |
| Aor13HI | TCCGGA | 1 cut(s) | 2067 |
| Aor51HI | AGCGCT | 1 cut(s) | 738 |
| AoxI | GGCC | 5 cut(s) | 517, 872, 908, 1156, 1480 |
| ApeKI | GCWGC | 4 cut(s) | 524, 763, 1295, 1469 |
| ApoI | RAATTY | 6 cut(s) | 84, 219, 399, 572, 1735, 2056 |
| AseI | ATTAAT | 1 cut(s) | 300 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2009 |
| AspLEI | GCGC | 1 cut(s) | 739 |
| AspS9I | GGNCC | 4 cut(s) | 908, 1322, 1538, 1604 |
| AsuHPI | GGTGA | 7 cut(s) | 301, 506, 521, 1298, 1401, 1941, 1972 |
| AvaII | GGWCC | 3 cut(s) | 1322, 1538, 1604 |
| AxyI | CCTNAGG | 1 cut(s) | 585 |
| BanII | GRGCYC | 1 cut(s) | 1594 |
| BbsI | GAAGAC | 2 cut(s) | 201, 1119 |
| BbvI | GCAGC | 4 cut(s) | 511, 750, 1282, 1456 |
| BccI | CCATC | 9 cut(s) | 646, 1023, 1112, 1277, 1312, 1559, 1595, 1657, 1809 |
| BceAI | ACGGC | 1 cut(s) | 1218 |
| BciT130I | CCWGG | 2 cut(s) | 1326, 1422 |
| BclI | TGATCA | 1 cut(s) | 1900 |
| BcoDI | GTCTC | 2 cut(s) | 1652, 2160 |
| BfaI | CTAG | 2 cut(s) | 1778, 1868 |
| BfmI | CTRYAG | 3 cut(s) | 1218, 1530, 2190 |
| BfoI | RGCGCY | 1 cut(s) | 740 |
| BisI | GCNGC | 5 cut(s) | 525, 764, 1159, 1296, 1470 |
| BlpI | GCTNAGC | 1 cut(s) | 1233 |
| BlsI | GCNGC | 5 cut(s) | 526, 765, 1160, 1297, 1471 |
| Bme1390I | CCNGG | 2 cut(s) | 1326, 1422 |
| Bme18I | GGWCC | 3 cut(s) | 1322, 1538, 1604 |
| BmgT120I | GGNCC | 4 cut(s) | 908, 1322, 1538, 1604 |
| BmiI | GGNNCC | 8 cut(s) | 158, 188, 488, 582, 1323, 1390, 1606, 1920 |
| BmrFI | CCNGG | 2 cut(s) | 1326, 1422 |
| BmsI | GCATC | 8 cut(s) | 651, 806, 1131, 1170, 1415, 1762, 2023, 2176 |
| BpiI | GAAGAC | 2 cut(s) | 201, 1119 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1706, 1738 |
| BpmI | CTGGAG | 2 cut(s) | 954, 2157 |
| Bpu10I | CCTNAGC | 2 cut(s) | 759, 816 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1233 |
| BpuEI | CTTGAG | 4 cut(s) | 215, 221, 443, 1041 |
| BsaAI | YACGTR | 1 cut(s) | 1500 |
| BsaBI | GATNNNNATC | 2 cut(s) | 641, 1014 |
| BsaJI | CCNNGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| BsaWI | WCCGGW | 2 cut(s) | 1891, 2067 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 932, 1535, 1891 |
| Bse118I | RCCGGY | 1 cut(s) | 1482 |
| Bse1I | ACTGG | 4 cut(s) | 64, 2027, 2140, 2180 |
| Bse21I | CCTNAGG | 1 cut(s) | 585 |
| Bse3DI | GCAATG | 2 cut(s) | 970, 2141 |
| Bse8I | GATNNNNATC | 2 cut(s) | 641, 1014 |
| BseAI | TCCGGA | 1 cut(s) | 2067 |
| BseBI | CCWGG | 2 cut(s) | 1326, 1422 |
| BseDI | CCNNGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| BseGI | GGATG | 7 cut(s) | 642, 688, 1015, 1161, 1672, 1760, 2191 |
| BseJI | GATNNNNATC | 2 cut(s) | 641, 1014 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 932, 1535, 1891 |
| BseMI | GCAATG | 2 cut(s) | 970, 2141 |
| BseMII | CTCAG | 6 cut(s) | 576, 750, 825, 1150, 1224, 2165 |
| BseNI | ACTGG | 4 cut(s) | 64, 2027, 2140, 2180 |
| BseRI | GAGGAG | 3 cut(s) | 111, 218, 1731 |
| BseXI | GCAGC | 4 cut(s) | 511, 750, 1282, 1456 |
| BsgI | GTGCAG | 1 cut(s) | 250 |
| Bsh1236I | CGCG | 1 cut(s) | 216 |
| BshFI | GGCC | 5 cut(s) | 519, 874, 910, 1158, 1482 |
| BsiSI | CCGG | 3 cut(s) | 1483, 1892, 2068 |
| BslFI | GGGAC | 1 cut(s) | 1335 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 932, 1535, 1891 |
| BsmAI | GTCTC | 2 cut(s) | 1652, 2160 |
| BsmBI | CGTCTC | 1 cut(s) | 1652 |
| BsmFI | GGGAC | 1 cut(s) | 1335 |
| BsmI | GAATGC | 2 cut(s) | 686, 1069 |
| BsnI | GGCC | 5 cut(s) | 519, 874, 910, 1158, 1482 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1594 |
| Bsp13I | TCCGGA | 1 cut(s) | 2067 |
| Bsp1407I | TGTACA | 1 cut(s) | 1349 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1233 |
| Bsp19I | CCATGG | 2 cut(s) | 1477, 1704 |
| BspACI | CCGC | 3 cut(s) | 214, 1159, 1957 |
| BspANI | GGCC | 5 cut(s) | 519, 874, 910, 1158, 1482 |
| BspCNI | CTCAG | 6 cut(s) | 577, 751, 824, 1149, 1225, 2164 |
| BspEI | TCCGGA | 1 cut(s) | 2067 |
| BspFNI | CGCG | 1 cut(s) | 216 |
| BspHI | TCATGA | 2 cut(s) | 255, 2112 |
| BspLI | GGNNCC | 8 cut(s) | 158, 188, 488, 582, 1323, 1390, 1606, 1920 |
| BspPI | GGATC | 8 cut(s) | 47, 462, 749, 900, 1471, 1522, 1866, 1936 |
| BsrDI | GCAATG | 2 cut(s) | 970, 2141 |
| BsrFI | RCCGGY | 1 cut(s) | 1482 |
| BsrGI | TGTACA | 1 cut(s) | 1349 |
| BsrI | ACTGG | 4 cut(s) | 64, 2027, 2140, 2180 |
| BssAI | RCCGGY | 1 cut(s) | 1482 |
| BssECI | CCNNGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| BssT1I | CCWWGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| Bst2UI | CCWGG | 2 cut(s) | 1326, 1422 |
| Bst4CI | ACNGT | 3 cut(s) | 112, 342, 2099 |
| Bst6I | CTCTTC | 5 cut(s) | 591, 1055, 1485, 1683, 2163 |
| BstAUI | TGTACA | 1 cut(s) | 1349 |
| BstBAI | YACGTR | 1 cut(s) | 1500 |
| BstDSI | CCRYGG | 2 cut(s) | 1477, 1704 |
| BstEII | GGTNACC | 1 cut(s) | 655 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| BstF5I | GGATG | 7 cut(s) | 642, 688, 1015, 1161, 1672, 1760, 2191 |
| BstFNI | CGCG | 1 cut(s) | 216 |
| BstH2I | RGCGCY | 1 cut(s) | 740 |
| BstHHI | GCGC | 1 cut(s) | 739 |
| BstMAI | GTCTC | 2 cut(s) | 1652, 2160 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 960 |
| BstNI | CCWGG | 2 cut(s) | 1326, 1422 |
| BstPI | GGTNACC | 1 cut(s) | 655 |
| BstSCI | CCNGG | 2 cut(s) | 1324, 1420 |
| BstSFI | CTRYAG | 3 cut(s) | 1218, 1530, 2190 |
| BstSNI | TACGTA | 1 cut(s) | 1500 |
| BstUI | CGCG | 1 cut(s) | 216 |
| BstV1I | GCAGC | 4 cut(s) | 511, 750, 1282, 1456 |
| BstV2I | GAAGAC | 2 cut(s) | 201, 1119 |
| BstX2I | RGATCY | 5 cut(s) | 454, 754, 892, 1463, 1928 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1565 |
| BstYI | RGATCY | 5 cut(s) | 454, 754, 892, 1463, 1928 |
| Bsu36I | CCTNAGG | 1 cut(s) | 585 |
| BsuRI | GGCC | 5 cut(s) | 519, 874, 910, 1158, 1482 |
| BtgI | CCRYGG | 2 cut(s) | 1477, 1704 |
| BtsCI | GGATG | 7 cut(s) | 642, 688, 1015, 1161, 1672, 1760, 2191 |
| BtsI | GCAGTG | 1 cut(s) | 384 |
| BtsIMutI | CAGTG | 4 cut(s) | 384, 1454, 2133, 2187 |
| CciI | TCATGA | 2 cut(s) | 255, 2112 |
| CfoI | GCGC | 1 cut(s) | 739 |
| Cfr10I | RCCGGY | 1 cut(s) | 1482 |
| Cfr13I | GGNCC | 4 cut(s) | 908, 1322, 1538, 1604 |
| CsiI | ACCWGGT | 1 cut(s) | 1324 |
| Csp6I | GTAC | 4 cut(s) | 330, 1304, 1350, 1576 |
| CviQI | GTAC | 4 cut(s) | 330, 1304, 1350, 1576 |
| DraI | TTTAAA | 1 cut(s) | 240 |
| EaeI | YGGCCR | 1 cut(s) | 1480 |
| Eam1104I | CTCTTC | 5 cut(s) | 591, 1055, 1485, 1683, 2163 |
| EarI | CTCTTC | 5 cut(s) | 591, 1055, 1485, 1683, 2163 |
| Eco105I | TACGTA | 1 cut(s) | 1500 |
| Eco130I | CCWWGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| Eco24I | GRGCYC | 1 cut(s) | 1594 |
| Eco32I | GATATC | 2 cut(s) | 1243, 1585 |
| Eco47I | GGWCC | 3 cut(s) | 1322, 1538, 1604 |
| Eco47III | AGCGCT | 1 cut(s) | 738 |
| Eco57I | CTGAAG | 3 cut(s) | 213, 827, 1430 |
| Eco81I | CCTNAGG | 1 cut(s) | 585 |
| Eco91I | GGTNACC | 1 cut(s) | 655 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1322 |
| EcoO65I | GGTNACC | 1 cut(s) | 655 |
| EcoRI | GAATTC | 1 cut(s) | 572 |
| EcoRII | CCWGG | 2 cut(s) | 1324, 1420 |
| EcoRV | GATATC | 2 cut(s) | 1243, 1585 |
| EcoT14I | CCWWGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| EcoT22I | ATGCAT | 1 cut(s) | 1408 |
| EcoT38I | GRGCYC | 1 cut(s) | 1594 |
| ErhI | CCWWGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| Esp3I | CGTCTC | 1 cut(s) | 1652 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 153, 185, 910, 942 |
| FaqI | GGGAC | 1 cut(s) | 1335 |
| FauI | CCCGC | 1 cut(s) | 1950 |
| FauNDI | CATATG | 1 cut(s) | 1384 |
| FbaI | TGATCA | 1 cut(s) | 1900 |
| Fnu4HI | GCNGC | 5 cut(s) | 525, 764, 1159, 1296, 1470 |
| FokI | GGATG | 7 cut(s) | 649, 675, 1002, 1148, 1679, 1747, 2198 |
| FriOI | GRGCYC | 1 cut(s) | 1594 |
| Fsp4HI | GCNGC | 5 cut(s) | 525, 764, 1159, 1296, 1470 |
| FspBI | CTAG | 2 cut(s) | 1778, 1868 |
| GlaI | GCGC | 1 cut(s) | 738 |
| GluI | GCNGC | 5 cut(s) | 525, 764, 1159, 1296, 1470 |
| GsuI | CTGGAG | 2 cut(s) | 954, 2157 |
| HaeII | RGCGCY | 1 cut(s) | 740 |
| HaeIII | GGCC | 5 cut(s) | 519, 874, 910, 1158, 1482 |
| HapII | CCGG | 3 cut(s) | 1483, 1892, 2068 |
| HhaI | GCGC | 1 cut(s) | 739 |
| Hin6I | GCGC | 1 cut(s) | 737 |
| HinP1I | GCGC | 1 cut(s) | 737 |
| HincII | GTYRAC | 2 cut(s) | 367, 619 |
| HindII | GTYRAC | 2 cut(s) | 367, 619 |
| HindIII | AAGCTT | 1 cut(s) | 167 |
| HinfI | GANTC | 3 cut(s) | 445, 1610, 1636 |
| HpaI | GTTAAC | 1 cut(s) | 367 |
| HpaII | CCGG | 3 cut(s) | 1483, 1892, 2068 |
| HphI | GGTGA | 7 cut(s) | 301, 506, 521, 1298, 1401, 1941, 1972 |
| Hpy166II | GTNNAC | 4 cut(s) | 321, 367, 619, 1541 |
| Hpy8I | GTNNAC | 4 cut(s) | 321, 367, 619, 1541 |
| Hpy99I | CGWCG | 2 cut(s) | 1107, 2090 |
| HpyAV | CCTTC | 5 cut(s) | 131, 1148, 1505, 1529, 1677 |
| HpyCH4III | ACNGT | 3 cut(s) | 112, 342, 2099 |
| HpyCH4IV | ACGT | 1 cut(s) | 1499 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 960 |
| HpySE526I | ACGT | 1 cut(s) | 1499 |
| HspAI | GCGC | 1 cut(s) | 737 |
| Kpn2I | TCCGGA | 1 cut(s) | 2067 |
| Ksp22I | TGATCA | 1 cut(s) | 1900 |
| KspAI | GTTAAC | 1 cut(s) | 367 |
| LmnI | GCTCC | 5 cut(s) | 98, 586, 820, 1909, 2138 |
| Lsp1109I | GCAGC | 4 cut(s) | 511, 750, 1282, 1456 |
| LweI | GCATC | 8 cut(s) | 651, 806, 1131, 1170, 1415, 1762, 2023, 2176 |
| MabI | ACCWGGT | 1 cut(s) | 1324 |
| MaeI | CTAG | 2 cut(s) | 1778, 1868 |
| MaeII | ACGT | 1 cut(s) | 1499 |
| MaeIII | GTNAC | 4 cut(s) | 307, 464, 655, 1669 |
| MflI | RGATCY | 5 cut(s) | 454, 754, 892, 1463, 1928 |
| MhlI | GDGCHC | 1 cut(s) | 1594 |
| MmeI | TCCRAC | 1 cut(s) | 541 |
| Mph1103I | ATGCAT | 1 cut(s) | 1408 |
| MroI | TCCGGA | 1 cut(s) | 2067 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2009 |
| MseI | TTAA | 9 cut(s) | 12, 239, 300, 366, 414, 1130, 1428, 1934, 2200 |
| MslI | CAYNNNNRTG | 4 cut(s) | 314, 429, 1020, 1563 |
| MspI | CCGG | 3 cut(s) | 1483, 1892, 2068 |
| MspR9I | CCNGG | 2 cut(s) | 1326, 1422 |
| Mva1269I | GAATGC | 2 cut(s) | 686, 1069 |
| MvaI | CCWGG | 2 cut(s) | 1326, 1422 |
| MvnI | CGCG | 1 cut(s) | 216 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 960 |
| NcoI | CCATGG | 2 cut(s) | 1477, 1704 |
| NdeI | CATATG | 1 cut(s) | 1384 |
| NlaIV | GGNNCC | 8 cut(s) | 158, 188, 488, 582, 1323, 1390, 1606, 1920 |
| NmuCI | GTSAC | 2 cut(s) | 307, 464 |
| NsiI | ATGCAT | 1 cut(s) | 1408 |
| PagI | TCATGA | 2 cut(s) | 255, 2112 |
| PctI | GAATGC | 2 cut(s) | 686, 1069 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2009 |
| PfeI | GAWTC | 3 cut(s) | 445, 1610, 1636 |
| PkrI | GCNGC | 5 cut(s) | 526, 765, 1160, 1297, 1471 |
| Ppu21I | YACGTR | 1 cut(s) | 1500 |
| PpuMI | RGGWCCY | 1 cut(s) | 1322 |
| PshBI | ATTAAT | 1 cut(s) | 300 |
| PsiI | TTATAA | 1 cut(s) | 1700 |
| Psp5II | RGGWCCY | 1 cut(s) | 1322 |
| Psp6I | CCWGG | 2 cut(s) | 1324, 1420 |
| PspEI | GGTNACC | 1 cut(s) | 655 |
| PspGI | CCWGG | 2 cut(s) | 1324, 1420 |
| PspN4I | GGNNCC | 8 cut(s) | 158, 188, 488, 582, 1323, 1390, 1606, 1920 |
| PspPI | GGNCC | 4 cut(s) | 908, 1322, 1538, 1604 |
| PspPPI | RGGWCCY | 1 cut(s) | 1322 |
| PsuI | RGATCY | 5 cut(s) | 454, 754, 892, 1463, 1928 |
| RsaI | GTAC | 4 cut(s) | 331, 1305, 1351, 1577 |
| RsaNI | GTAC | 4 cut(s) | 330, 1304, 1350, 1576 |
| RseI | CAYNNNNRTG | 4 cut(s) | 314, 429, 1020, 1563 |
| SaqAI | TTAA | 9 cut(s) | 12, 239, 300, 366, 414, 1130, 1428, 1934, 2200 |
| SatI | GCNGC | 5 cut(s) | 525, 764, 1159, 1296, 1470 |
| Sau96I | GGNCC | 4 cut(s) | 908, 1322, 1538, 1604 |
| ScrFI | CCNGG | 2 cut(s) | 1326, 1422 |
| SduI | GDGCHC | 1 cut(s) | 1594 |
| SexAI | ACCWGGT | 1 cut(s) | 1324 |
| SfaNI | GCATC | 8 cut(s) | 651, 806, 1131, 1170, 1415, 1762, 2023, 2176 |
| SfcI | CTRYAG | 3 cut(s) | 1218, 1530, 2190 |
| SinI | GGWCC | 3 cut(s) | 1322, 1538, 1604 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 314, 429, 1020, 1563 |
| SmlI | CTYRAG | 4 cut(s) | 200, 230, 458, 1056 |
| SmoI | CTYRAG | 4 cut(s) | 200, 230, 458, 1056 |
| SnaBI | TACGTA | 1 cut(s) | 1500 |
| SsiI | CCGC | 3 cut(s) | 214, 1159, 1957 |
| SspMI | CTAG | 2 cut(s) | 1778, 1868 |
| StyD4I | CCNGG | 2 cut(s) | 1324, 1420 |
| StyI | CCWWGG | 4 cut(s) | 490, 1477, 1704, 1725 |
| TaaI | ACNGT | 3 cut(s) | 112, 342, 2099 |
| TaiI | ACGT | 1 cut(s) | 1502 |
| TaqI | TCGA | 1 cut(s) | 2085 |
| TatI | WGTACW | 1 cut(s) | 1349 |
| TauI | GCSGC | 1 cut(s) | 1161 |
| TfiI | GAWTC | 3 cut(s) | 445, 1610, 1636 |
| Tru1I | TTAA | 9 cut(s) | 12, 239, 300, 366, 414, 1130, 1428, 1934, 2200 |
| Tru9I | TTAA | 9 cut(s) | 12, 239, 300, 366, 414, 1130, 1428, 1934, 2200 |
| TscAI | CASTG | 4 cut(s) | 384, 1454, 2140, 2187 |
| TseFI | GTSAC | 2 cut(s) | 307, 464 |
| TseI | GCWGC | 4 cut(s) | 524, 763, 1295, 1469 |
| Tsp45I | GTSAC | 2 cut(s) | 307, 464 |
| TspGWI | ACGGA | 1 cut(s) | 1120 |
| TspRI | CASTG | 4 cut(s) | 384, 1454, 2140, 2187 |
| VpaK11BI | GGWCC | 3 cut(s) | 1322, 1538, 1604 |
| VspI | ATTAAT | 1 cut(s) | 300 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| XapI | RAATTY | 6 cut(s) | 84, 219, 399, 572, 1735, 2056 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2009 |
| XspI | CTAG | 2 cut(s) | 1778, 1868 |
| Zsp2I | ATGCAT | 1 cut(s) | 1408 |