Rorug02G0637900

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000368
Physical Location & Seq
Forward (+)
75097407 .. 75099790
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0637900.1

Sequence Viewer

Length: 1380 bp
ATGGGAACTCAAGCTCCAACCGACGAAAATTCTTCCATTCCGAGCAATATGGAGGAGCAATCAGAGAAGCAATCACAGATAGATGAAAAGCTAGAGAGGCAGAAGCAAATTGATGCTTGGCTTCCAATCACTTCCTCAAGGAATGCAAACTGGTGGCACTCAGCTTTCCATAACGTCAACTCCATGGTTGGAGTTGGTGTCCTAAGTCTTCCTTATGCCATGTCGAATCTAGGATGGGGTCCTGGTGTAGTTGCTTTAGTACTATCATGGGTCATCACTTTATTCACATTATGGCAAATGGTTGAGATGCATGAAATGGTACCTGGAAAGCGATTCGATAGGTACCATGAACTGGGTCAACATGCCTTTGGTGAAAAGCTTGGTCTGTGGATTGTGGTGCCTCAACAGCTGGTCTGCGAAGTTGGTGTGGACATTGTGTATATGGTCGCAGGAGGAAAAACACTGCAGAAGGTGCATAGCATAGTTCGCAAAGGCCAGAACCCGATCAAACTCACCTACTTCATCATGATCTATGCCTCGGCTCAGTTTGTACTCGCACATCTCCCCAACTTTGGCTCCATTGCCGGCGTGTCCTTGACTGCAGCAGTCATGTGTTTAAGTTATTCTACTATTGCATGGACAGCTTCCGTACATAAGGGTGTTCAGGCAGATGTAGAATATGGGTATACAGCTCATAACGCATCGGGAACAGTTTTCAACTTCTTGACTGCCTTGGGAAACGTTGCGTTTGCCTATGCCGGCCATAATGTGGTTTTGGAAATCCAAGCAACAGTCCCTTCTTCACCTGGCAAGCCATCCAAGAAGCCTATGTGGAGAGGGGTGCTCATTGCCTATACAGTGGTCGCTTTGTGCTATTTCCCTGTTGCCCTCATCGGATTTTATACTTTCGGCAATAAAGTCGAGGACAACATCCTAATCTCCTTGGAGAAACCTGCATGGCTAGTCATTACGGCCAACATGTTTGTAGTTATTCATATTATTGGAGGTTATCAGCTATTTGCAATGCCAGTATTCGACATGATAGAAACAGTCCTGGTAAAGAAACTGCATTTTAGGCCTACAAGAACTCTTCGCTTCATTTCGAGGAATGTATACGTTGCACTCACAATGTTCGTGGGCATTAGTTTTCCCTTCTTTGGTGGACTTCTGGGATTCTTTGGAGGATTTGCTTTTTCGCCAACAACATACTTTCTCCCTTGCATTATCTGGCTAGCCATAAGGAAACCACGGAAGTTTAGCTTATCTTGGTTCATTAACTGGATTTGCATTACACTGGGTGTCCTGCTCATGATCCTATCACCTATTGGAGGGTTAAGGCATATAATACTAGAAGCCAAGGACTACCATTTCTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

459

Amino Acids

51.25

Weight (kDa)

9.01

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 48 - 443 3.6e-105 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 60 - 201 8.9e-08 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 961
Acc65I GGTACC 2 cut(s) 319, 342
AccB1I GGYRCC 3 cut(s) 319, 342, 397
AccB7I CCANNNNNTGG 2 cut(s) 352, 769
AccI GTMKAC 2 cut(s) 686, 1113
AclI AACGTT 1 cut(s) 741
AclWI GGATC 1 cut(s) 1306
AcoI YGGCCR 2 cut(s) 760, 972
AcsI RAATTY 1 cut(s) 28
AdeI CACNNNGTG 1 cut(s) 1298
AfaI GTAC 5 cut(s) 261, 321, 344, 552, 651
AfiI CCNNNNNNNGG 5 cut(s) 352, 572, 769, 1157, 1328
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 1 cut(s) 718
AjnI CCWGG 4 cut(s) 241, 322, 805, 1053
AloI GAACNNNNNNTCC 1 cut(s) 30
AluBI AGCT 9 cut(s) 14, 91, 164, 379, 409, 644, 692, 1015, 1260
AluI AGCT 9 cut(s) 14, 91, 164, 379, 409, 644, 692, 1015, 1260
Alw21I GWGCWC 1 cut(s) 846
AlwI GGATC 1 cut(s) 1306
AoxI GGCC 4 cut(s) 493, 760, 972, 1076
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 1 cut(s) 28
Asp718I GGTACC 2 cut(s) 319, 342
AspS9I GGNCC 1 cut(s) 239
AsuHPI GGTGA 4 cut(s) 383, 505, 795, 1311
AsuNHI GCTAGC 1 cut(s) 1231
AvaII GGWCC 1 cut(s) 239
BanI GGYRCC 3 cut(s) 319, 342, 397
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 1 cut(s) 614
BccI CCATC 2 cut(s) 228, 823
BceAI ACGGC 1 cut(s) 987
BcgI CGANNNNNNTGC 2 cut(s) 901, 935
BciT130I CCWGG 4 cut(s) 243, 324, 807, 1055
BfaI CTAG 5 cut(s) 92, 230, 962, 1232, 1349
BfmI CTRYAG 2 cut(s) 464, 600
BfuAI ACCTGC 1 cut(s) 961
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
BmcAI AGTACT 1 cut(s) 261
Bme1390I CCNGG 4 cut(s) 243, 324, 807, 1055
Bme18I GGWCC 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 5 cut(s) 240, 321, 344, 399, 577
BmrFI CCNGG 4 cut(s) 243, 324, 807, 1055
BmrI ACTGGG 2 cut(s) 362, 1304
BmsI GCATC 3 cut(s) 103, 297, 710
BmtI GCTAGC 1 cut(s) 1235
BmuI ACTGGG 2 cut(s) 362, 1304
BpiI GAAGAC 1 cut(s) 200
BplI GAGNNNNNCTC 2 cut(s) 828, 860
BpuEI CTTGAG 1 cut(s) 121
BsaJI CCNNGG 6 cut(s) 183, 537, 732, 942, 1247, 1356
BsaXI ACNNNNNCTCC 7 cut(s) 28, 164, 183, 194, 213, 560, 590
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 572, 769, 1157, 1328
Bse118I RCCGGY 2 cut(s) 584, 758
Bse1I ACTGG 5 cut(s) 155, 357, 1028, 1283, 1299
Bse3DI GCAATG 3 cut(s) 579, 846, 1029
BseBI CCWGG 4 cut(s) 243, 324, 807, 1055
BseDI CCNNGG 6 cut(s) 183, 537, 732, 942, 1247, 1356
BseGI GGATG 3 cut(s) 239, 815, 930
BseLI CCNNNNNNNGG 5 cut(s) 352, 572, 769, 1157, 1328
BseMI GCAATG 3 cut(s) 579, 846, 1029
BseMII CTCAG 2 cut(s) 174, 557
BseNI ACTGG 5 cut(s) 155, 357, 1028, 1283, 1299
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 1 cut(s) 614
BshFI GGCC 4 cut(s) 495, 762, 974, 1078
BshNI GGYRCC 3 cut(s) 319, 342, 397
BsiHKAI GWGCWC 1 cut(s) 846
BsiSI CCGG 2 cut(s) 585, 759
BslFI GGGAC 1 cut(s) 779
BslI CCNNNNNNNGG 5 cut(s) 352, 572, 769, 1157, 1328
BsmFI GGGAC 1 cut(s) 779
BsmI GAATGC 1 cut(s) 148
BsnI GGCC 4 cut(s) 495, 762, 974, 1078
Bsp1286I GDGCHC 1 cut(s) 846
Bsp143I GATC 3 cut(s) 504, 528, 1311
Bsp19I CCATGG 1 cut(s) 183
BspANI GGCC 4 cut(s) 495, 762, 974, 1078
BspCNI CTCAG 2 cut(s) 173, 556
BspHI TCATGA 2 cut(s) 525, 1308
BspLI GGNNCC 5 cut(s) 240, 321, 344, 399, 577
BspMAI CTGCAG 2 cut(s) 468, 604
BspMI ACCTGC 1 cut(s) 961
BspOI GCTAGC 1 cut(s) 1235
BspPI GGATC 1 cut(s) 1306
BspT107I GGYRCC 3 cut(s) 319, 342, 397
BsrDI GCAATG 3 cut(s) 579, 846, 1029
BsrFI RCCGGY 2 cut(s) 584, 758
BsrI ACTGG 5 cut(s) 155, 357, 1028, 1283, 1299
BssAI RCCGGY 2 cut(s) 584, 758
BssECI CCNNGG 6 cut(s) 183, 537, 732, 942, 1247, 1356
BssMI GATC 3 cut(s) 504, 528, 1311
BssNAI GTATAC 2 cut(s) 687, 1114
BssT1I CCWWGG 4 cut(s) 183, 732, 942, 1356
Bst1107I GTATAC 2 cut(s) 687, 1114
Bst2UI CCWGG 4 cut(s) 243, 324, 807, 1055
Bst4CI ACNGT 4 cut(s) 712, 793, 859, 1051
Bst6I CTCTTC 1 cut(s) 1095
BstAPI GCANNNNNTGC 1 cut(s) 472
BstC8I GCNNGC 4 cut(s) 586, 760, 812, 1233
BstDEI CTNAG 3 cut(s) 160, 203, 543
BstDSI CCRYGG 2 cut(s) 183, 1247
BstENI CCTNNNNNAGG 1 cut(s) 1326
BstF5I GGATG 3 cut(s) 239, 815, 930
BstKTI GATC 3 cut(s) 507, 531, 1314
BstMBI GATC 3 cut(s) 504, 528, 1311
BstMWI GCNNNNNNNGC 7 cut(s) 97, 406, 472, 486, 641, 698, 1075
BstNI CCWGG 4 cut(s) 243, 324, 807, 1055
BstNSI RCATGY 2 cut(s) 365, 982
BstSCI CCNGG 4 cut(s) 241, 322, 805, 1053
BstSFI CTRYAG 2 cut(s) 464, 600
BstV1I GCAGC 1 cut(s) 614
BstV2I GAAGAC 1 cut(s) 200
BstZ17I GTATAC 2 cut(s) 687, 1114
BsuRI GGCC 4 cut(s) 495, 762, 974, 1078
BtgI CCRYGG 2 cut(s) 183, 1247
BtsCI GGATG 3 cut(s) 239, 815, 930
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 3 cut(s) 461, 864, 1292
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 4 cut(s) 586, 760, 812, 1233
CciI TCATGA 2 cut(s) 525, 1308
Cfr10I RCCGGY 2 cut(s) 584, 758
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 5 cut(s) 260, 320, 343, 551, 650
CspCI CAANNNNNGTGG 2 cut(s) 1116, 1151
CviQI GTAC 5 cut(s) 260, 320, 343, 551, 650
DdeI CTNAG 3 cut(s) 160, 203, 543
DpnI GATC 3 cut(s) 506, 530, 1313
DpnII GATC 3 cut(s) 504, 528, 1311
DraIII CACNNNGTG 1 cut(s) 1298
EaeI YGGCCR 2 cut(s) 760, 972
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
Eco130I CCWWGG 4 cut(s) 183, 732, 942, 1356
Eco147I AGGCCT 1 cut(s) 1078
Eco47I GGWCC 1 cut(s) 239
EcoNI CCTNNNNNAGG 1 cut(s) 1326
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 4 cut(s) 241, 322, 805, 1053
EcoT14I CCWWGG 4 cut(s) 183, 732, 942, 1356
EcoT22I ATGCAT 1 cut(s) 312
ErhI CCWWGG 4 cut(s) 183, 732, 942, 1356
FalI AAGNNNNNCTT 4 cut(s) 196, 228, 1244, 1276
FaqI GGGAC 1 cut(s) 779
FblI GTMKAC 2 cut(s) 686, 1113
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 3 cut(s) 246, 802, 917
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 5 cut(s) 92, 230, 962, 1232, 1349
GluI GCNGC 1 cut(s) 603
HaeIII GGCC 4 cut(s) 495, 762, 974, 1078
HapII CCGG 2 cut(s) 585, 759
HincII GTYRAC 2 cut(s) 178, 359
HindII GTYRAC 2 cut(s) 178, 359
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 226, 333, 1173
HpaII CCGG 2 cut(s) 585, 759
HphI GGTGA 4 cut(s) 383, 505, 795, 1311
Hpy166II GTNNAC 6 cut(s) 178, 359, 430, 687, 1114, 1163
Hpy188I TCNGA 3 cut(s) 42, 64, 896
Hpy188III TCNNGA 4 cut(s) 526, 705, 724, 1309
Hpy8I GTNNAC 6 cut(s) 178, 359, 430, 687, 1114, 1163
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 463, 807, 1162
HpyCH4III ACNGT 4 cut(s) 712, 793, 859, 1051
HpyCH4IV ACGT 3 cut(s) 174, 741, 1116
HpyF10VI GCNNNNNNNGC 7 cut(s) 97, 406, 472, 486, 641, 698, 1075
HpyF3I CTNAG 3 cut(s) 160, 203, 543
HpySE526I ACGT 3 cut(s) 174, 741, 1116
KpnI GGTACC 2 cut(s) 323, 346
KroI GCCGGC 2 cut(s) 584, 758
KroNI GCCGGC 2 cut(s) 586, 760
Kzo9I GATC 3 cut(s) 504, 528, 1311
LmnI GCTCC 3 cut(s) 19, 55, 581
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 3 cut(s) 103, 297, 710
MaeI CTAG 5 cut(s) 92, 230, 962, 1232, 1349
MaeII ACGT 3 cut(s) 174, 741, 1116
MalI GATC 3 cut(s) 506, 530, 1313
MboI GATC 3 cut(s) 504, 528, 1311
MboII GAAGA 4 cut(s) 24, 200, 792, 1082
MhlI GDGCHC 1 cut(s) 846
MluCI AATT 3 cut(s) 28, 108, 1375
MmeI TCCRAC 2 cut(s) 41, 169
Mph1103I ATGCAT 1 cut(s) 312
MroNI GCCGGC 2 cut(s) 584, 758
MseI TTAA 4 cut(s) 617, 1275, 1334, 1378
MslI CAYNNNNRTG 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 409
MspI CCGG 2 cut(s) 585, 759
MspR9I CCNGG 4 cut(s) 243, 324, 807, 1055
Mva1269I GAATGC 1 cut(s) 148
MvaI CCWGG 4 cut(s) 243, 324, 807, 1055
MwoI GCNNNNNNNGC 7 cut(s) 97, 406, 472, 486, 641, 698, 1075
NaeI GCCGGC 2 cut(s) 586, 760
NcoI CCATGG 1 cut(s) 183
NdeII GATC 3 cut(s) 504, 528, 1311
NgoMIV GCCGGC 2 cut(s) 584, 758
NheI GCTAGC 1 cut(s) 1231
NlaIV GGNNCC 5 cut(s) 240, 321, 344, 399, 577
NmeAIII GCCGAG 1 cut(s) 518
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 2 cut(s) 365, 982
PagI TCATGA 2 cut(s) 525, 1308
PceI AGGCCT 1 cut(s) 1078
PciI ACATGT 1 cut(s) 978
PctI GAATGC 1 cut(s) 148
PdiI GCCGGC 2 cut(s) 586, 760
PfeI GAWTC 3 cut(s) 226, 333, 1173
PflMI CCANNNNNTGG 2 cut(s) 352, 769
PkrI GCNGC 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 978
Psp1406I AACGTT 1 cut(s) 741
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 4 cut(s) 241, 322, 805, 1053
PspGI CCWGG 4 cut(s) 241, 322, 805, 1053
PspN4I GGNNCC 5 cut(s) 240, 321, 344, 399, 577
PspPI GGNCC 1 cut(s) 239
PspPPI RGGWCCY 1 cut(s) 239
PstI CTGCAG 2 cut(s) 468, 604
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 5 cut(s) 261, 321, 344, 552, 651
RsaNI GTAC 5 cut(s) 260, 320, 343, 551, 650
RseI CAYNNNNRTG 1 cut(s) 657
SaqAI TTAA 4 cut(s) 617, 1275, 1334, 1378
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 3 cut(s) 504, 528, 1311
Sau96I GGNCC 1 cut(s) 239
ScaI AGTACT 1 cut(s) 261
ScrFI CCNGG 4 cut(s) 243, 324, 807, 1055
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 3 cut(s) 103, 297, 710
SfcI CTRYAG 2 cut(s) 464, 600
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 657
SmlI CTYRAG 2 cut(s) 9, 136
SmoI CTYRAG 2 cut(s) 9, 136
Sse9I AATT 3 cut(s) 28, 108, 1375
SseBI AGGCCT 1 cut(s) 1078
SspMI CTAG 5 cut(s) 92, 230, 962, 1232, 1349
StuI AGGCCT 1 cut(s) 1078
StyD4I CCNGG 4 cut(s) 241, 322, 805, 1053
StyI CCWWGG 4 cut(s) 183, 732, 942, 1356
TaaI ACNGT 4 cut(s) 712, 793, 859, 1051
TaiI ACGT 3 cut(s) 177, 744, 1119
TaqI TCGA 5 cut(s) 224, 336, 921, 1035, 1103
TasI AATT 3 cut(s) 28, 108, 1375
TatI WGTACW 2 cut(s) 259, 550
TfiI GAWTC 3 cut(s) 226, 333, 1173
Tru1I TTAA 4 cut(s) 617, 1275, 1334, 1378
Tru9I TTAA 4 cut(s) 617, 1275, 1334, 1378
TscAI CASTG 3 cut(s) 468, 864, 1299
TseI GCWGC 1 cut(s) 602
TspDTI ATGAA 7 cut(s) 99, 327, 363, 511, 983, 1087, 1261
TspGWI ACGGA 2 cut(s) 637, 1264
TspRI CASTG 3 cut(s) 468, 864, 1299
Van91I CCANNNNNTGG 2 cut(s) 352, 769
VpaK11BI GGWCC 1 cut(s) 239
XagI CCTNNNNNAGG 1 cut(s) 1326
XapI RAATTY 1 cut(s) 28
XceI RCATGY 2 cut(s) 365, 982
XmiI GTMKAC 2 cut(s) 686, 1113
XspI CTAG 5 cut(s) 92, 230, 962, 1232, 1349
ZrmI AGTACT 1 cut(s) 261
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.