Rorug01G0052400

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000368
Physical Location & Seq
Forward (+)
8657658 .. 8671874
14217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0052400.1

Sequence Viewer

Length: 3342 bp
ATGGCTTCTTCTATGATGGGTCAACTGGTTTCTCCCTTGGAGAATGGACATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAAGAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAGTTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCACACCGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCACGGATTTGATCGGCCTATATATACTAATGTTGTATATCCATTTCCACTTGATCCACCTTTTGTTCCTGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATCACTGATTATTGTTATCCTTGTGGGACGAACAAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAGGATCATTGGTGGTTATCTGGCATTCATCGTGATGTGCTTCTTCTTTCCAAGCCACAGGTATTTATTGCAGATTATTTCTTCAAATCTAATCTGGCTGAGGATTTCTCTTATGCAGACATACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCTAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAACTGGTACAGTACTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCACCCAGCACAATTCCTGGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGCCGAATCTGTACACTCTGGTTGTGATATTGAAAGATACATCTGGCAATATTGTGGACTGTGAATCATGCCTAGTAGGCATACGGCAAGTATCAAAAGCCCCTAAGCAGCTGCTTGTTAATGGGCATCCGATAATAATAAGGGGTGTGAACAGGCATGAGCATCATCCACGTCTGGGGAAGACAAACATAGAATCGTGCATGGTTAAGGATTTGGTTTTAATGAAGCAATACAATATCAATGCTGTAAGAAACAGCCATTATCCTCAACATCCTCGCTGGTATGAGCTTTGTGACATGTTTGGCATGTACATGATAGATGAAGCCAATATTGAAACCCATGGTTTCGATTATACTGGACATGTGAAGCATCCGACTTTGGAACCCAGTTGGGCAACTGCAATGTTGGACCGTGTGATAGGCATGGTTGAGAGGGACAAAAATCATGCGTGCATCATCTCTTGGTCCTTGGGAAATGAATCTGGATATGGACCTAATCATTCTGCTTTAGCTGGTTGGGTTCGTGGAAAGGATCCTTCACGGCTGTTACATTACGAAGGGGGAGGATCCAGAACCCCGTCTACAGATATTATATGCCCCATGTATATGCGTGTTTGGGACATTGTGAAGATTGCGAAAGATCCAAATGAAACACGTCCTTTAATACTGTGCGAGTATTCACATGCAATGGGAAATAGCAATGGAAATATACATGAATATTGGGAAGCAATTGATGGCACATTTGGTCTTCAAGGAGGCTTTATCTGGGATTGGGTTGATCAGGGCTTACTGAAAGATAGTGCTGATGGTACTAAACACTGGGCATATGGTGGTGACTTTGGTGATGTTCCTAATGATTTAAACTTCTGTTTGAATGGCCTTGTGTGGCCCGATCGAACTCCTCATCCTGCAATGCATGAAGTGAAGTATGTGTACCAGCCAATCAAGGTTTCATTTAGTGAAGGAACACTAAAGATAACAAATACTCACTTCTATGAAACAACAAAAGCATTGGAATTCTGCTGGGCTGCTCATGGAGATGGATGTGAACTTGGATCTGGAAATTTATCCCTTCCCTTGATTGAACCCCAGAAGACTTTTCATATTGAGTTGCAGTCAGCTCCGTGGCATACTCTATGGGCTTCATCATCAGCAGAGGAGTTTTTCTTAACAATAAGTGCTAAGCTTTTGCATTCCACATGCTGGGTTGAAGCTGGTCATGTCATCTCATCTACACAAGTCCAGTTGCCTGCCAAAAGAGAATTTGTTCCTCATGTAGTCATCAAGACTAAAGATACTACATTCCTTAAGGAAATTCTTGGAGATACACTCAAAGTTGGCCAACAGAATGCCTGGGAGATAATACTGAATCTTAAAATGGGAACAGTTGAAAGCTGGAAGGTGGAAGGAGTTCCTTTGATGACAAAAGGCATATTTCCATGTTTTTGGCGAGCACCCACAGATAATGACAAAGGCGGAGGTGCCAGTAGTTATTCGTCCAAGTGGCAAGCTGCTCATATTGATAGCCTTCATTATATTACCAAGAGCTGTTCTGTACAGAATATGACTGACCATCTTGTGAAAGTAGCTGTTGTTTTTCTCGGTGTTCCAAACAGTGGGGACGGTTCTGGGGTGGAAGACACAAATGCCTTAATTGAGATTGATGTGATTTACACAATCTACGGTTCAGGGGACGTTGTTGTTGAATGCAATGTCAGACCAAGTTCGAACCTTCCGCCTCTGCCACGTGTGGGAGTTGAGTTCCACTTGGAGAAGTCAATAGACCAGATTAAATGGTACGGAAGAGGACCATTTGAATGTTATCCAGACAGAAAAGCAGCTGCTCATGTTGGAGTCTATGAGCAGAAAGTGAGTGATCTGCATGTCCCTTATATAGTTCCTGGGGAATGTTCAGGTAGGGCAGACGTTAGGTGGGTGACATTTCAAAACAAGGATGGTTTGGGAATCTATGCTTCAATATATGGCAGCTCTCCACCCATGCAAATAAATGCAAGTTATTACACCACAGCTGAGCTGGATCGGGCAACACACAATGAAGATCTTATCAGAGGAGATGACATTGAGGTTCATCTTGATCACAAGCACATGGGCCTGGCCGGGGATGATAGTTGGTCTCCATGTGTCCATGACAAGTATCTGATTCCTCCTGTTCCAAGCTCATTCTCAGTTAGGCTCTCGCCAATAACTGCTGCAACCTCTGGCCATGATATCTACAAATCTCAACTTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

1113

Amino Acids

125.15

Weight (kDa)

5.59

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 260 1.6e-54 Glycosyl hydrolases family 2, sugar binding domain
Glyco_hydro_2 PF00703 262 - 391 4.1e-13 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 398 - 678 1.7e-97 Glycosyl hydrolases family 2, TIM barrel domain
LacZ_4 PF16353 686 - 779 3e-18 Beta-galactosidase, domain 4
Bgal_small_N PF02929 811 - 1094 4.1e-73 Beta galactosidase small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2567
AccB7I CCANNNNNTGG 3 cut(s) 513, 2289, 2702
AccI GTMKAC 2 cut(s) 610, 1667
AccII CGCG 1 cut(s) 341
AciI CCGC 3 cut(s) 339, 2562, 2822
AcoI YGGCCR 3 cut(s) 2425, 3201, 3307
AcsI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2400
AcuI CTGAAG 1 cut(s) 237
AcvI CACGTG 1 cut(s) 2834
AfaI GTAC 9 cut(s) 675, 947, 952, 1100, 1397, 1897, 2021, 2643, 2885
AfiI CCNNNNNNNGG 6 cut(s) 513, 1262, 2289, 2702, 2837, 3205
AflII CTTAAG 1 cut(s) 2393
AflIII ACRYGT 5 cut(s) 100, 1383, 1447, 1739, 2833
AjiI CACGTC 2 cut(s) 1259, 1742
AjnI CCWGG 5 cut(s) 307, 1024, 2438, 2986, 3198
AjuI GAANNNNNNNTTGG 2 cut(s) 2020, 2052
AloI GAACNNNNNNTCC 2 cut(s) 1975, 2007
Alw21I GWGCWC 1 cut(s) 2542
Alw26I GTCTC 2 cut(s) 1048, 3225
AoxI GGCC 7 cut(s) 407, 1963, 1973, 2425, 3196, 3201, 3307
ApoI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2400
Asp700I GAANNNNTTC 2 cut(s) 2352, 2496
AspS9I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2894, 3196
AsuC2I CCSGG 1 cut(s) 3205
AsuHPI GGTGA 4 cut(s) 1002, 1931, 1940, 3034
AsuII TTCGAA 2 cut(s) 543, 2813
AvaII GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2894
BalI TGGCCA 2 cut(s) 2427, 3309
BamHI GGATCC 2 cut(s) 1618, 1652
BanI GGYRCC 1 cut(s) 2567
BarI GAAGNNNNNNTAC 2 cut(s) 2003, 2035
BbrPI CACGTG 1 cut(s) 2834
BbsI GAAGAC 4 cut(s) 1274, 1826, 2186, 2730
Bbv12I GWGCWC 1 cut(s) 2542
BbvCI CCTCAGC 1 cut(s) 822
BccI CCATC 8 cut(s) 10, 690, 698, 1814, 1886, 2120, 2667, 3035
BceAI ACGGC 2 cut(s) 1187, 1643
BciT130I CCWGG 5 cut(s) 309, 1026, 2440, 2988, 3200
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 2 cut(s) 1864, 3181
BcnI CCSGG 1 cut(s) 3205
BcoDI GTCTC 2 cut(s) 1048, 3225
BfaI CTAG 2 cut(s) 677, 1160
BfmI CTRYAG 6 cut(s) 484, 588, 912, 1080, 1668, 3338
BfrI CTTAAG 1 cut(s) 2393
BfuI GTATCC 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 1164
BglII AGATCT 1 cut(s) 3145
BlpI GCTNAGC 2 cut(s) 2268, 3117
BmcAI AGTACT 1 cut(s) 952
Bme1390I CCNGG 6 cut(s) 309, 1026, 2440, 2988, 3200, 3205
Bme18I GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2894
BmgBI CACGTC 2 cut(s) 1259, 1742
BmgT120I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2894, 3196
BmiI GGNNCC 4 cut(s) 1470, 1620, 1654, 2569
BmrFI CCNGG 6 cut(s) 309, 1026, 2440, 2988, 3200, 3205
BmrI ACTGGG 2 cut(s) 1467, 1915
BmsI GCATC 7 cut(s) 85, 196, 381, 1222, 1258, 1465, 1548
BmuI ACTGGG 2 cut(s) 1467, 1915
BpiI GAAGAC 4 cut(s) 1274, 1826, 2186, 2730
BplI GAGNNNNNCTC 4 cut(s) 815, 847, 2400, 2432
Bpu10I CCTNAGC 2 cut(s) 822, 1191
Bpu1102I GCTNAGC 2 cut(s) 2268, 3117
Bpu14I TTCGAA 2 cut(s) 543, 2813
BpuMI CCSGG 1 cut(s) 3205
BsaAI YACGTR 1 cut(s) 2834
BsaBI GATNNNNATC 1 cut(s) 726
BsaI GGTCTC 1 cut(s) 3225
BsaJI CCNNGG 9 cut(s) 36, 194, 1067, 1426, 1554, 2210, 2439, 2987, 3204
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 573, 603
Bsc4I CCNNNNNNNGG 6 cut(s) 513, 1262, 2289, 2702, 2837, 3205
Bse3DI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2803
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 5 cut(s) 309, 1026, 2440, 2988, 3200
BseDI CCNNGG 9 cut(s) 36, 194, 1067, 1426, 1554, 2210, 2439, 2987, 3204
BseGI GGATG 9 cut(s) 501, 1213, 1252, 1357, 1456, 1990, 2135, 3046, 3214
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 6 cut(s) 513, 1262, 2289, 2702, 2837, 3205
BseMI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2803
BseMII CTCAG 4 cut(s) 345, 813, 3108, 3285
BseRI GAGGAG 3 cut(s) 1977, 2258, 3171
BseYI CCCAGC 3 cut(s) 1013, 2109, 2289
BsgI GTGCAG 2 cut(s) 174, 252
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 1981
BshFI GGCC 7 cut(s) 409, 1965, 1975, 2427, 3198, 3203, 3309
BshNI GGYRCC 1 cut(s) 2567
BsiEI CGRYCG 1 cut(s) 1981
BsiHKAI GWGCWC 1 cut(s) 2542
BsiSI CCGG 1 cut(s) 3204
BslFI GGGAC 6 cut(s) 670, 1535, 1718, 2720, 2792, 2957
BslI CCNNNNNNNGG 6 cut(s) 513, 1262, 2289, 2702, 2837, 3205
BsmAI GTCTC 2 cut(s) 1048, 3225
BsmFI GGGAC 6 cut(s) 670, 1535, 1718, 2720, 2792, 2957
BsmI GAATGC 4 cut(s) 747, 2278, 2440, 2798
BsnI GGCC 7 cut(s) 409, 1965, 1975, 2427, 3198, 3203, 3309
Bso31I GGTCTC 1 cut(s) 3225
Bsp119I TTCGAA 2 cut(s) 543, 2813
Bsp1286I GDGCHC 1 cut(s) 2542
Bsp1407I TGTACA 3 cut(s) 1098, 1395, 2641
Bsp1720I GCTNAGC 2 cut(s) 2268, 3117
Bsp19I CCATGG 1 cut(s) 1426
BspACI CCGC 3 cut(s) 339, 2562, 2822
BspANI GGCC 7 cut(s) 409, 1965, 1975, 2427, 3198, 3203, 3309
BspCNI CTCAG 4 cut(s) 346, 814, 3109, 3284
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 4 cut(s) 1470, 1620, 1654, 2569
BspMAI CTGCAG 1 cut(s) 1084
BspT104I TTCGAA 2 cut(s) 543, 2813
BspT107I GGYRCC 1 cut(s) 2567
BspTI CTTAAG 1 cut(s) 2393
BspTNI GGTCTC 1 cut(s) 3225
BsrDI GCAATG 5 cut(s) 1494, 1779, 1792, 2004, 2803
BsrGI TGTACA 3 cut(s) 1098, 1395, 2641
BssECI CCNNGG 9 cut(s) 36, 194, 1067, 1426, 1554, 2210, 2439, 2987, 3204
BssT1I CCWWGG 5 cut(s) 36, 194, 1067, 1426, 1554
Bst2UI CCWGG 5 cut(s) 309, 1026, 2440, 2988, 3200
Bst6I CTCTTC 2 cut(s) 83, 2884
BstAFI CTTAAG 1 cut(s) 2393
BstAUI TGTACA 3 cut(s) 1098, 1395, 2641
BstBAI YACGTR 1 cut(s) 2834
BstBI TTCGAA 2 cut(s) 543, 2813
BstC8I GCNNGC 5 cut(s) 254, 1537, 2337, 2538, 2595
BstDEI CTNAG 7 cut(s) 295, 354, 822, 1191, 2268, 3117, 3271
BstDSI CCRYGG 2 cut(s) 1426, 2210
BstF5I GGATG 9 cut(s) 501, 1213, 1252, 1357, 1456, 1990, 2135, 3046, 3214
BstFNI CGCG 1 cut(s) 341
BstMAI GTCTC 2 cut(s) 1048, 3225
BstMCI CGRYCG 1 cut(s) 1981
BstMWI GCNNNNNNNGC 2 cut(s) 566, 1164
BstNI CCWGG 5 cut(s) 309, 1026, 2440, 2988, 3200
BstNSI RCATGY 7 cut(s) 104, 1387, 1396, 1451, 1772, 2289, 2972
BstSCI CCNGG 6 cut(s) 307, 1024, 2438, 2986, 3198, 3203
BstSFI CTRYAG 6 cut(s) 484, 588, 912, 1080, 1668, 3338
BstUI CGCG 1 cut(s) 341
BstV2I GAAGAC 4 cut(s) 1274, 1826, 2186, 2730
BstX2I RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3145
BstXI CCANNNNNNTGG 2 cut(s) 1074, 2532
BstYI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3145
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 7 cut(s) 409, 1965, 1975, 2427, 3198, 3203, 3309
BtgI CCRYGG 2 cut(s) 1426, 2210
BtrI CACGTC 2 cut(s) 1259, 1742
BtsCI GGATG 9 cut(s) 501, 1213, 1252, 1357, 1456, 1990, 2135, 3046, 3214
BtsIMutI CAGTG 5 cut(s) 633, 898, 940, 1903, 2707
Cac8I GCNNGC 5 cut(s) 254, 1537, 2337, 2538, 2595
Cfr13I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2894, 3196
Csp6I GTAC 9 cut(s) 674, 946, 951, 1099, 1396, 1896, 2020, 2642, 2884
CviQI GTAC 9 cut(s) 674, 946, 951, 1099, 1396, 1896, 2020, 2642, 2884
DdeI CTNAG 7 cut(s) 295, 354, 822, 1191, 2268, 3117, 3271
DraI TTTAAA 1 cut(s) 1947
EaeI YGGCCR 3 cut(s) 2425, 3201, 3307
Eam1104I CTCTTC 2 cut(s) 83, 2884
EarI CTCTTC 2 cut(s) 83, 2884
EciI GGCGGA 2 cut(s) 2577, 2811
Eco130I CCWWGG 5 cut(s) 36, 194, 1067, 1426, 1554
Eco31I GGTCTC 1 cut(s) 3225
Eco32I GATATC 1 cut(s) 3316
Eco47I GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2894
Eco57I CTGAAG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 2834
EcoRI GAATTC 1 cut(s) 2102
EcoRII CCWGG 5 cut(s) 307, 1024, 2438, 2986, 3198
EcoRV GATATC 1 cut(s) 3316
EcoT14I CCWWGG 5 cut(s) 36, 194, 1067, 1426, 1554
EcoT22I ATGCAT 1 cut(s) 2004
ErhI CCWWGG 5 cut(s) 36, 194, 1067, 1426, 1554
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 6 cut(s) 670, 1535, 1718, 2720, 2792, 2957
FauNDI CATATG 1 cut(s) 1912
FbaI TGATCA 2 cut(s) 1864, 3181
FblI GTMKAC 2 cut(s) 610, 1667
FokI GGATG 9 cut(s) 488, 1200, 1239, 1344, 1443, 1977, 2142, 3053, 3221
FspBI CTAG 2 cut(s) 677, 1160
GsaI CCCAGC 3 cut(s) 1017, 2113, 2293
HaeIII GGCC 7 cut(s) 409, 1965, 1975, 2427, 3198, 3203, 3309
HapII CCGG 1 cut(s) 3204
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 2 cut(s) 998, 2270
HpaII CCGG 1 cut(s) 3204
HphI GGTGA 4 cut(s) 1002, 1931, 1940, 3034
Hpy166II GTNNAC 9 cut(s) 23, 466, 611, 1101, 1144, 1237, 1668, 2020, 2135
Hpy188I TCNGA 6 cut(s) 355, 1218, 1461, 2804, 3155, 3246
Hpy8I GTNNAC 9 cut(s) 23, 466, 611, 1101, 1144, 1237, 1668, 2020, 2135
HpyCH4IV ACGT 6 cut(s) 112, 1258, 1741, 2781, 2833, 3012
HpyF10VI GCNNNNNNNGC 2 cut(s) 566, 1164
HpyF3I CTNAG 7 cut(s) 295, 354, 822, 1191, 2268, 3117, 3271
HpySE526I ACGT 6 cut(s) 112, 1258, 1741, 2781, 2833, 3012
Ksp22I TGATCA 2 cut(s) 1864, 3181
LmnI GCTCC 1 cut(s) 2212
LweI GCATC 7 cut(s) 85, 196, 381, 1222, 1258, 1465, 1548
MaeI CTAG 2 cut(s) 677, 1160
MaeII ACGT 6 cut(s) 112, 1258, 1741, 2781, 2833, 3012
MaeIII GTNAC 5 cut(s) 103, 1379, 1632, 1919, 3022
MfeI CAATTG 1 cut(s) 1815
MflI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3145
MhlI GDGCHC 1 cut(s) 2542
MlsI TGGCCA 2 cut(s) 2427, 3309
MluNI TGGCCA 2 cut(s) 2427, 3309
MlyI GAGTC 2 cut(s) 275, 2949
MmeI TCCRAC 4 cut(s) 207, 1473, 1484, 2917
Mox20I TGGCCA 2 cut(s) 2427, 3309
Mph1103I ATGCAT 1 cut(s) 2004
MroXI GAANNNNTTC 2 cut(s) 2352, 2496
MscI TGGCCA 2 cut(s) 2427, 3309
MslI CAYNNNNRTG 6 cut(s) 756, 1397, 1691, 2079, 2124, 2902
Msp20I TGGCCA 2 cut(s) 2427, 3309
MspA1I CMGCKG 3 cut(s) 1198, 2927, 3116
MspCI CTTAAG 1 cut(s) 2393
MspI CCGG 1 cut(s) 3204
MspR9I CCNGG 6 cut(s) 309, 1026, 2440, 2988, 3200, 3205
MunI CAATTG 1 cut(s) 1815
Mva1269I GAATGC 4 cut(s) 747, 2278, 2440, 2798
MvaI CCWGG 5 cut(s) 309, 1026, 2440, 2988, 3200
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 2 cut(s) 566, 1164
NciI CCSGG 1 cut(s) 3205
NcoI CCATGG 1 cut(s) 1426
NdeI CATATG 1 cut(s) 1912
NlaIV GGNNCC 4 cut(s) 1470, 1620, 1654, 2569
NmuCI GTSAC 4 cut(s) 103, 1379, 1919, 3022
NsiI ATGCAT 1 cut(s) 2004
NspI RCATGY 7 cut(s) 104, 1387, 1396, 1451, 1772, 2289, 2972
NspV TTCGAA 2 cut(s) 543, 2813
PciI ACATGT 3 cut(s) 100, 1383, 1447
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PctI GAATGC 4 cut(s) 747, 2278, 2440, 2798
PdmI GAANNNNTTC 2 cut(s) 2352, 2496
PflMI CCANNNNNTGG 3 cut(s) 513, 2289, 2702
PfoI TCCNGGA 2 cut(s) 307, 1024
Ple19I CGATCG 1 cut(s) 1981
PleI GAGTC 2 cut(s) 274, 2948
PmaCI CACGTG 1 cut(s) 2834
PmlI CACGTG 1 cut(s) 2834
PpsI GAGTC 2 cut(s) 274, 2948
Ppu21I YACGTR 1 cut(s) 2834
PscI ACATGT 3 cut(s) 100, 1383, 1447
Psp6I CCWGG 5 cut(s) 307, 1024, 2438, 2986, 3198
PspCI CACGTG 1 cut(s) 2834
PspFI CCCAGC 3 cut(s) 1013, 2109, 2289
PspGI CCWGG 5 cut(s) 307, 1024, 2438, 2986, 3198
PspN4I GGNNCC 4 cut(s) 1470, 1620, 1654, 2569
PspPI GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2894, 3196
PsrI GAACNNNNNNTAC 4 cut(s) 666, 698, 1651, 1683
PstI CTGCAG 1 cut(s) 1084
PsuI RGATCY 7 cut(s) 134, 959, 1618, 1652, 1726, 2141, 3145
PvuI CGATCG 1 cut(s) 1981
PvuII CAGCTG 3 cut(s) 1198, 2927, 3116
RsaI GTAC 9 cut(s) 675, 947, 952, 1100, 1397, 1897, 2021, 2643, 2885
RsaNI GTAC 9 cut(s) 674, 946, 951, 1099, 1396, 1896, 2020, 2642, 2884
RseI CAYNNNNRTG 6 cut(s) 756, 1397, 1691, 2079, 2124, 2902
SalI GTCGAC 1 cut(s) 609
Sau96I GGNCC 7 cut(s) 64, 1495, 1551, 1577, 1974, 2894, 3196
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 2 cut(s) 275, 2949
ScrFI CCNGG 6 cut(s) 309, 1026, 2440, 2988, 3200, 3205
SduI GDGCHC 1 cut(s) 2542
SfaNI GCATC 7 cut(s) 85, 196, 381, 1222, 1258, 1465, 1548
SfcI CTRYAG 6 cut(s) 484, 588, 912, 1080, 1668, 3338
SfuI TTCGAA 2 cut(s) 543, 2813
SinI GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2894
SmiMI CAYNNNNRTG 6 cut(s) 756, 1397, 1691, 2079, 2124, 2902
SmlI CTYRAG 1 cut(s) 2393
SmoI CTYRAG 1 cut(s) 2393
SsiI CCGC 3 cut(s) 339, 2562, 2822
SspI AATATT 3 cut(s) 1138, 1417, 1805
SspMI CTAG 2 cut(s) 677, 1160
StyD4I CCNGG 6 cut(s) 307, 1024, 2438, 2986, 3198, 3203
StyI CCWWGG 5 cut(s) 36, 194, 1067, 1426, 1554
TaiI ACGT 6 cut(s) 115, 1261, 1744, 2784, 2836, 3015
TaqI TCGA 6 cut(s) 543, 552, 610, 1434, 1981, 2813
TatI WGTACW 5 cut(s) 673, 950, 1098, 1395, 2641
TscAI CASTG 5 cut(s) 640, 898, 940, 1910, 2707
TseFI GTSAC 4 cut(s) 103, 1379, 1919, 3022
Tsp45I GTSAC 4 cut(s) 103, 1379, 1919, 3022
TspGWI ACGGA 3 cut(s) 411, 2199, 2901
TspRI CASTG 5 cut(s) 640, 898, 940, 1910, 2707
Van91I CCANNNNNTGG 3 cut(s) 513, 2289, 2702
Vha464I CTTAAG 1 cut(s) 2393
VpaK11BI GGWCC 5 cut(s) 64, 1495, 1551, 1577, 2894
XapI RAATTY 9 cut(s) 169, 287, 325, 528, 623, 2102, 2149, 2348, 2400
XceI RCATGY 7 cut(s) 104, 1387, 1396, 1451, 1772, 2289, 2972
XcmI CCANNNNNNNNNTGG 1 cut(s) 3118
XmiI GTMKAC 2 cut(s) 610, 1667
XmnI GAANNNNTTC 2 cut(s) 2352, 2496
XspI CTAG 2 cut(s) 677, 1160
ZrmI AGTACT 1 cut(s) 952
Zsp2I ATGCAT 1 cut(s) 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.