FvH4_6g03500

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000368
Physical Location & Seq
Reverse (-)
1919981 .. 1921999
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03500.t1

Sequence Viewer

Length: 510 bp
ATGGCTCTCACAGCGCTCAAGGGACCTGCAACGGCGGCCGTACTGCTCTGTTTTCTGTTGACGTATCATCTGAATCCGGCATTGGCTGCCACAGCCGGGTCGGATGATAATTCGGATTCGGATTCGGATTCATATCGGTTTTCCACCGATTTCGTTTCATATCCACGGCGGGGTCCACCGGAGGAGCGTAAAGGCCAGCAGTGGATTTTCGGTTGGTTGATTATGGGAGCATTTGCGGTGCTGGTGTTCAAGTGTTCAACATACGGTAGATCTACCGTAATCAAACTTCAGGTTGGTGAGTCCGGCACGGCGAGAACACTCCAGAAGGATCTTGATCAGATGCCTAAAATTGAGTATGCATGTTTTCCTCAAAAGGATTTGAGCTGTGTTTTGACAGGTGCAACAGCAGCTTTGCTTCGCCATTCTCATGATTGCATATCAGGATATTCTTCTGTTGCTACCATACGAAATATATATTCTGCATACAGTAAAATACTACAATCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

170

Amino Acids

18.44

Weight (kDa)

8.27

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 92 - 163 4.6e-06 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 34
AciI CCGC 3 cut(s) 35, 169, 236
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 272
AfaI GTAC 1 cut(s) 42
AfeI AGCGCT 1 cut(s) 15
AfiI CCNNNNNNNGG 2 cut(s) 96, 170
AgsI TTSAA 2 cut(s) 250, 258
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AluBI AGCT 2 cut(s) 384, 410
AluI AGCT 2 cut(s) 384, 410
AlwI GGATC 1 cut(s) 336
Aor51HI AGCGCT 1 cut(s) 15
AoxI GGCC 2 cut(s) 36, 193
ApeKI GCWGC 2 cut(s) 86, 407
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 23, 173
AsuC2I CCSGG 1 cut(s) 97
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 2 cut(s) 23, 173
BarI GAAGNNNNNNTAC 2 cut(s) 270, 302
BbvI GCAGC 2 cut(s) 73, 419
BceAI ACGGC 4 cut(s) 23, 48, 182, 324
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 97
BfoI RGCGCY 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 34
BglII AGATCT 1 cut(s) 269
BisI GCNGC 3 cut(s) 36, 87, 408
BlsI GCNGC 3 cut(s) 37, 88, 409
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 2 cut(s) 23, 173
BmgT120I GGNCC 2 cut(s) 23, 173
BmiI GGNNCC 2 cut(s) 24, 174
BmrFI CCNGG 1 cut(s) 97
BmsI GCATC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 1 cut(s) 97
BsaBI GATNNNNATC 2 cut(s) 132, 333
BsaJI CCNNGG 1 cut(s) 164
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 170
Bse8I GATNNNNATC 2 cut(s) 132, 333
BseDI CCNNGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 109
BseJI GATNNNNATC 2 cut(s) 132, 333
BseLI CCNNNNNNNGG 2 cut(s) 96, 170
BseRI GAGGAG 1 cut(s) 197
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 73, 419
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 2 cut(s) 38, 195
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 4 cut(s) 77, 96, 179, 303
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 96, 170
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 2 cut(s) 38, 195
Bsp143I GATC 3 cut(s) 269, 328, 334
BspACI CCGC 3 cut(s) 35, 169, 236
BspANI GGCC 2 cut(s) 38, 195
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 2 cut(s) 24, 174
BspMI ACCTGC 1 cut(s) 34
BspPI GGATC 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 164
BssMI GATC 3 cut(s) 269, 328, 334
Bst4CI ACNGT 3 cut(s) 266, 277, 488
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 1 cut(s) 109
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 3 cut(s) 272, 331, 337
BstMBI GATC 3 cut(s) 269, 328, 334
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 5 cut(s) 11, 35, 86, 92, 407
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 73, 419
BstX2I RGATCY 2 cut(s) 269, 328
BstYI RGATCY 2 cut(s) 269, 328
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 2 cut(s) 38, 195
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 206
BtsIMutI CAGTG 1 cut(s) 206
BveI ACCTGC 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 197
CciI TCATGA 1 cut(s) 427
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 23, 173
Csp6I GTAC 1 cut(s) 41
CviAII CATG 2 cut(s) 360, 428
CviJI RGCY 7 cut(s) 5, 38, 86, 95, 195, 384, 410
CviKI_1 RGCY 7 cut(s) 5, 38, 86, 95, 195, 384, 410
CviQI GTAC 1 cut(s) 41
DpnI GATC 3 cut(s) 271, 330, 336
DpnII GATC 3 cut(s) 269, 328, 334
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
EclXI CGGCCG 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 23, 173
Eco47III AGCGCT 1 cut(s) 15
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 23
EcoT22I ATGCAT 1 cut(s) 361
FaeI CATG 2 cut(s) 363, 431
FaqI GGGAC 1 cut(s) 36
FatI CATG 2 cut(s) 359, 427
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 36, 87, 408
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 3 cut(s) 36, 87, 408
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 36, 87, 408
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 2 cut(s) 38, 195
HapII CCGG 4 cut(s) 77, 96, 179, 303
HhaI GCGC 1 cut(s) 16
Hin1II CATG 2 cut(s) 363, 431
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HinfI GANTC 5 cut(s) 73, 116, 122, 128, 299
HpaII CCGG 4 cut(s) 77, 96, 179, 303
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 2 cut(s) 60, 176
Hpy188I TCNGA 6 cut(s) 72, 103, 115, 121, 127, 339
Hpy188III TCNNGA 4 cut(s) 322, 332, 428, 441
Hpy8I GTNNAC 2 cut(s) 60, 176
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 3 cut(s) 266, 277, 488
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 29, 359, 401, 435, 482
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 35, 86, 92, 407
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 2 cut(s) 363, 431
HspAI GCGC 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 269, 328, 334
LmnI GCTCC 2 cut(s) 184, 227
Lsp1109I GCAGC 2 cut(s) 73, 419
LweI GCATC 1 cut(s) 330
MaeII ACGT 1 cut(s) 62
MalI GATC 3 cut(s) 271, 330, 336
MboI GATC 3 cut(s) 269, 328, 334
MboII GAAGA 1 cut(s) 441
MflI RGATCY 2 cut(s) 269, 328
MluCI AATT 3 cut(s) 109, 348, 505
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 2 cut(s) 175, 378
Mph1103I ATGCAT 1 cut(s) 361
MslI CAYNNNNRTG 1 cut(s) 426
MspI CCGG 4 cut(s) 77, 96, 179, 303
MspR9I CCNGG 1 cut(s) 97
MwoI GCNNNNNNNGC 5 cut(s) 11, 35, 86, 92, 407
NciI CCSGG 1 cut(s) 97
NdeII GATC 3 cut(s) 269, 328, 334
NlaIII CATG 2 cut(s) 363, 431
NlaIV GGNNCC 2 cut(s) 24, 174
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 1 cut(s) 363
PagI TCATGA 1 cut(s) 427
PfeI GAWTC 4 cut(s) 73, 116, 122, 128
PkrI GCNGC 3 cut(s) 37, 88, 409
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 23
PspN4I GGNNCC 2 cut(s) 24, 174
PspPI GGNCC 2 cut(s) 23, 173
PspPPI RGGWCCY 1 cut(s) 23
PsuI RGATCY 2 cut(s) 269, 328
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 426
SatI GCNGC 3 cut(s) 36, 87, 408
Sau3AI GATC 3 cut(s) 269, 328, 334
Sau96I GGNCC 2 cut(s) 23, 173
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 97
SetI ASST 6 cut(s) 28, 65, 294, 386, 400, 412
SfaNI GCATC 1 cut(s) 330
SinI GGWCC 2 cut(s) 23, 173
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 3 cut(s) 109, 348, 505
SsiI CCGC 3 cut(s) 35, 169, 236
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 266, 277, 488
TaiI ACGT 1 cut(s) 65
TaqI TCGA 1 cut(s) 503
TasI AATT 3 cut(s) 109, 348, 505
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 4 cut(s) 73, 116, 122, 128
TscAI CASTG 1 cut(s) 206
TseI GCWGC 2 cut(s) 86, 407
TspDTI ATGAA 2 cut(s) 120, 147
TspRI CASTG 1 cut(s) 206
VpaK11BI GGWCC 2 cut(s) 23, 173
XceI RCATGY 1 cut(s) 363
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.