MD04G1220400.v1.1

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000368
Physical Location & Seq
Forward (+)
30144558 .. 30148145
3588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1220400.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGGCGGCAGCAGCTTCGTTGCTCCAACCCAACCCATTGAAATGGAAGCAGCACAATCTACTCCCCCGTCTCCGCCCCCTCCCCACAAACCCAATCAAATTCAAAAACTTCAATTTCCAAATCCAAAAAATCCCCTGCAAATTCAAAGTTCAATGCTTTAAGCAGTTATCCGACTCAAAATCCCAGTCCCTTGTCGTCGAAGAAAACCCAGCAAACCCAATTGGGATTATCGCCGGAAAGCTGTTGGAAGCTATGAAGGCGTTGAGAAAGCCGGCAATGGTCGCCGTGTTGTTGGGTCTGCTGCTGATGTCCGACCCCAATTCGGCATTGGCCGCCTCGGGCGGTCGGGTCGGGGGAAGCTCGTTTTCTTCGCGCTCGTCGTCTTCCTCTTCGTCCTCGAGGAGTTACTCGGTGCCGAGGACTCCGGGCTCAAGGCCCGGTTTTTCGTACTCCGCGCCGTACTACGCGCCTTCGCCTTTCGGGTTCGGTGGCGGGGGTGGTGGATTTTACGTGGGGCCGGCATTTGGGTTCGGAGTTGGAGCCGGGTCGAGCTTCTTCCTGATCTTGACGGGTTTTGCGGCGTTTGTTTTGGTTTCCGGGTTCCTTTCGGATCGGTCTGAGGGAAGTGTGCTTACTGCTACTGAGAAAACAAGCGTTCTCAAGCTTCAGGTTGGTTTGTTGGGCATGGGACGAACGCTCCAAAGGGATCTTAATCGGATTGCTGAAACTGCTGATACTTCTACCCCTGATGGTTTGGGCTATGTTTTGACAGAGACAACACTAGCTTTGCTACGACATCCAGATTATTGCATCTCCGGTCATTCATCTGCTGCTCTAAAGCGAGGCATAGAGGATGCAGAGAAACGCTTTAATCAGCTTTCTATCGAAGAGAGGGGTAAATTTGACGAAGAGACACTTGTCAATGTGAACAACATAAGAAAGCAAAGCTCAACGAGCCGGAGTGCTAATGGATTCCGCAACGAATATATAGTGATAACAATATTGGTGGCTGCTGAAGGAGTGCATAAGTTGCCTGCCATCAATGGCAGTGGTGACTTGAAGGAAGCATTGCAAAAGCTTGGATCCATTCCCTCGAACAAAATAATGGCAGTAGAGGTCTTGTGGACTCCTCAGAATGAAACTGACACTCTGTCTGAGCGGGAACTACTTGAAGACTACCCGCTTTTGAGGCCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

400

Amino Acids

42.86

Weight (kDa)

9.44

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 217 - 399 1.1e-70 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 397
AccB1I GGYRCC 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 1159
AccII CGCG 3 cut(s) 373, 455, 467
AclWI GGATC 4 cut(s) 618, 714, 1077, 1090
AcoI YGGCCR 1 cut(s) 330
AcsI RAATTY 3 cut(s) 98, 140, 899
AcuI CTGAAG 2 cut(s) 650, 1035
AfaI GTAC 2 cut(s) 449, 461
AfiI CCNNNNNNNGG 2 cut(s) 322, 524
AgsI TTSAA 7 cut(s) 40, 103, 112, 145, 152, 1060, 1172
AhdI GACNNNNNGTC 1 cut(s) 1150
Alw26I GTCTC 3 cut(s) 74, 767, 905
AlwI GGATC 4 cut(s) 618, 714, 1077, 1090
Ama87I CYCGRG 2 cut(s) 337, 397
AoxI GGCC 4 cut(s) 330, 434, 515, 1190
ApeKI GCWGC 6 cut(s) 8, 11, 49, 301, 830, 1010
ApoI RAATTY 3 cut(s) 98, 140, 899
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspLEI GCGC 3 cut(s) 375, 457, 469
AspS9I GGNCC 2 cut(s) 435, 515
AsuC2I CCSGG 4 cut(s) 426, 438, 544, 598
AsuHPI GGTGA 1 cut(s) 1064
AvaI CYCGRG 2 cut(s) 337, 397
BamHI GGATCC 1 cut(s) 1082
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 431
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BbsI GAAGAC 2 cut(s) 375, 1179
BbvI GCAGC 6 cut(s) 20, 23, 61, 288, 817, 997
BccI CCATC 2 cut(s) 743, 1046
BceAI ACGGC 2 cut(s) 269, 442
BcgI CGANNNNNNTGC 1 cut(s) 31
BcnI CCSGG 4 cut(s) 426, 438, 544, 598
BcoDI GTCTC 3 cut(s) 74, 767, 905
BfaI CTAG 1 cut(s) 782
BisI GCNGC 9 cut(s) 6, 9, 12, 50, 302, 333, 579, 831, 1011
BlsI GCNGC 9 cut(s) 7, 10, 13, 51, 303, 334, 580, 832, 1012
Bme1390I CCNGG 4 cut(s) 426, 438, 544, 598
BmeRI GACNNNNNGTC 1 cut(s) 1150
BmeT110I CYCGRG 2 cut(s) 337, 397
BmgT120I GGNCC 2 cut(s) 435, 515
BmiI GGNNCC 5 cut(s) 414, 516, 541, 602, 1084
BmrFI CCNGG 4 cut(s) 426, 438, 544, 598
BmrI ACTGGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 819, 844
BmuI ACTGGG 1 cut(s) 178
BoxI GACNNNNGTC 1 cut(s) 917
BpiI GAAGAC 2 cut(s) 375, 1179
BpuEI CTTGAG 2 cut(s) 415, 644
BpuMI CCSGG 4 cut(s) 426, 438, 544, 598
BsaAI YACGTR 1 cut(s) 511
BsaBI GATNNNNATC 1 cut(s) 711
BsaJI CCNNGG 2 cut(s) 336, 416
BsaWI WCCGGW 1 cut(s) 815
BsaXI ACNNNNNCTCC 2 cut(s) 681, 711
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 524
Bse118I RCCGGY 2 cut(s) 271, 517
Bse1I ACTGG 1 cut(s) 184
Bse3DI GCAATG 2 cut(s) 282, 1067
Bse8I GATNNNNATC 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 336, 416
BseGI GGATG 2 cut(s) 796, 859
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 322, 524
BseMI GCAATG 2 cut(s) 282, 1067
BseMII CTCAG 4 cut(s) 609, 633, 1145, 1146
BseNI ACTGG 1 cut(s) 184
BseRI GAGGAG 2 cut(s) 415, 1119
BseXI GCAGC 6 cut(s) 20, 23, 61, 288, 817, 997
BseYI CCCAGC 1 cut(s) 208
Bsh1236I CGCG 3 cut(s) 373, 455, 467
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 4 cut(s) 332, 436, 517, 1192
BshNI GGYRCC 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 346
BsiHKCI CYCGRG 2 cut(s) 337, 397
BsiSI CCGG 9 cut(s) 234, 272, 425, 438, 518, 543, 597, 816, 958
BslFI GGGAC 2 cut(s) 172, 702
BslI CCNNNNNNNGG 2 cut(s) 322, 524
BsmAI GTCTC 3 cut(s) 74, 767, 905
BsmBI CGTCTC 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 172, 702
BsnI GGCC 4 cut(s) 332, 436, 517, 1192
BsoBI CYCGRG 2 cut(s) 337, 397
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 4 cut(s) 561, 610, 706, 1082
BspANI GGCC 4 cut(s) 332, 436, 517, 1192
BspCNI CTCAG 4 cut(s) 610, 634, 1144, 1147
BspFNI CGCG 3 cut(s) 373, 455, 467
BspLI GGNNCC 5 cut(s) 414, 516, 541, 602, 1084
BspPI GGATC 4 cut(s) 618, 714, 1077, 1090
BspT107I GGYRCC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 1159
BsrDI GCAATG 2 cut(s) 282, 1067
BsrFI RCCGGY 2 cut(s) 271, 517
BsrI ACTGG 1 cut(s) 184
BssAI RCCGGY 2 cut(s) 271, 517
BssECI CCNNGG 2 cut(s) 336, 416
BssMI GATC 4 cut(s) 561, 610, 706, 1082
Bst6I CTCTTC 3 cut(s) 394, 882, 903
BstAPI GCANNNNNTGC 1 cut(s) 1030
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 3 cut(s) 273, 519, 1035
BstDEI CTNAG 4 cut(s) 618, 642, 1131, 1155
BstF5I GGATG 2 cut(s) 796, 859
BstFNI CGCG 3 cut(s) 373, 455, 467
BstHHI GCGC 3 cut(s) 375, 457, 469
BstKTI GATC 4 cut(s) 564, 613, 709, 1085
BstMAI GTCTC 3 cut(s) 74, 767, 905
BstMBI GATC 4 cut(s) 561, 610, 706, 1082
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 8 cut(s) 11, 257, 281, 332, 728, 954, 1030, 1189
BstPAI GACNNNNGTC 1 cut(s) 917
BstSCI CCNGG 4 cut(s) 424, 436, 542, 596
BstUI CGCG 3 cut(s) 373, 455, 467
BstV1I GCAGC 6 cut(s) 20, 23, 61, 288, 817, 997
BstV2I GAAGAC 2 cut(s) 375, 1179
BstX2I RGATCY 2 cut(s) 706, 1082
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 2 cut(s) 706, 1082
BsuRI GGCC 4 cut(s) 332, 436, 517, 1192
BtsCI GGATG 2 cut(s) 796, 859
BtsI GCAGTG 1 cut(s) 1054
BtsIMutI CAGTG 1 cut(s) 1054
Cac8I GCNNGC 3 cut(s) 273, 519, 1035
CfoI GCGC 3 cut(s) 375, 457, 469
Cfr10I RCCGGY 2 cut(s) 271, 517
Cfr13I GGNCC 2 cut(s) 435, 515
Csp6I GTAC 2 cut(s) 448, 460
CspCI CAANNNNNGTGG 4 cut(s) 987, 1022, 1030, 1065
CviAII CATG 1 cut(s) 685
CviQI GTAC 2 cut(s) 448, 460
DdeI CTNAG 4 cut(s) 618, 642, 1131, 1155
DpnI GATC 4 cut(s) 563, 612, 708, 1084
DpnII GATC 4 cut(s) 561, 610, 706, 1082
DriI GACNNNNNGTC 1 cut(s) 1150
EaeI YGGCCR 1 cut(s) 330
Eam1104I CTCTTC 3 cut(s) 394, 882, 903
Eam1105I GACNNNNNGTC 1 cut(s) 1150
EarI CTCTTC 3 cut(s) 394, 882, 903
EciI GGCGGA 1 cut(s) 62
Eco147I AGGCCT 1 cut(s) 1192
Eco24I GRGCYC 1 cut(s) 431
Eco57I CTGAAG 2 cut(s) 650, 1035
Eco88I CYCGRG 2 cut(s) 337, 397
EcoT38I GRGCYC 1 cut(s) 431
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 1 cut(s) 688
FaiI YATR 9 cut(s) 254, 686, 762, 848, 935, 987, 989, 1026, 1198
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 2 cut(s) 172, 702
FatI CATG 1 cut(s) 684
FauI CCCGC 3 cut(s) 485, 1152, 1188
Fnu4HI GCNGC 9 cut(s) 6, 9, 12, 50, 302, 333, 579, 831, 1011
FokI GGATG 2 cut(s) 783, 866
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 9 cut(s) 6, 9, 12, 50, 302, 333, 579, 831, 1011
FspBI CTAG 1 cut(s) 782
GlaI GCGC 3 cut(s) 374, 456, 468
GluI GCNGC 9 cut(s) 6, 9, 12, 50, 302, 333, 579, 831, 1011
GsaI CCCAGC 1 cut(s) 212
HaeIII GGCC 4 cut(s) 332, 436, 517, 1192
HapII CCGG 9 cut(s) 234, 272, 425, 438, 518, 543, 597, 816, 958
HhaI GCGC 3 cut(s) 375, 457, 469
Hin1II CATG 1 cut(s) 688
Hin6I GCGC 3 cut(s) 373, 455, 467
HinP1I GCGC 3 cut(s) 373, 455, 467
HindIII AAGCTT 2 cut(s) 662, 1076
HinfI GANTC 4 cut(s) 173, 421, 972, 1126
HpaII CCGG 9 cut(s) 234, 272, 425, 438, 518, 543, 597, 816, 958
HphI GGTGA 1 cut(s) 1064
Hpy166II GTNNAC 2 cut(s) 928, 1125
Hpy188I TCNGA 8 cut(s) 172, 313, 533, 610, 619, 717, 1134, 1156
Hpy188III TCNNGA 3 cut(s) 559, 565, 800
Hpy8I GTNNAC 2 cut(s) 928, 1125
Hpy99I CGWCG 2 cut(s) 200, 382
HpyAV CCTTC 4 cut(s) 250, 480, 1010, 1054
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 5 cut(s) 138, 810, 857, 1024, 1072
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 257, 281, 332, 728, 954, 1030, 1189
HpyF3I CTNAG 4 cut(s) 618, 642, 1131, 1155
HpySE526I ACGT 1 cut(s) 510
Hsp92II CATG 1 cut(s) 688
HspAI GCGC 3 cut(s) 373, 455, 467
KroI GCCGGC 2 cut(s) 271, 517
KroNI GCCGGC 2 cut(s) 273, 519
Kzo9I GATC 4 cut(s) 561, 610, 706, 1082
LmnI GCTCC 3 cut(s) 27, 539, 702
Lsp1109I GCAGC 6 cut(s) 20, 23, 61, 288, 817, 997
LweI GCATC 2 cut(s) 819, 844
MaeI CTAG 1 cut(s) 782
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 2 cut(s) 404, 1052
MalI GATC 4 cut(s) 563, 612, 708, 1084
MbiI CCGCTC 1 cut(s) 1159
MboI GATC 4 cut(s) 561, 610, 706, 1082
MboII GAAGA 8 cut(s) 212, 360, 375, 381, 547, 899, 920, 1184
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 2 cut(s) 706, 1082
MhlI GDGCHC 1 cut(s) 431
MluCI AATT 6 cut(s) 98, 112, 140, 219, 319, 899
MlyI GAGTC 3 cut(s) 167, 415, 1120
MmeI TCCRAC 5 cut(s) 49, 195, 225, 336, 517
MroNI GCCGGC 2 cut(s) 271, 517
MseI TTAA 3 cut(s) 159, 711, 870
MslI CAYNNNNRTG 1 cut(s) 40
MspI CCGG 9 cut(s) 234, 272, 425, 438, 518, 543, 597, 816, 958
MspR9I CCNGG 4 cut(s) 426, 438, 544, 598
MunI CAATTG 1 cut(s) 219
MvnI CGCG 3 cut(s) 373, 455, 467
MwoI GCNNNNNNNGC 8 cut(s) 11, 257, 281, 332, 728, 954, 1030, 1189
NaeI GCCGGC 2 cut(s) 273, 519
NciI CCSGG 4 cut(s) 426, 438, 544, 598
NdeII GATC 4 cut(s) 561, 610, 706, 1082
NgoMIV GCCGGC 2 cut(s) 271, 517
NlaIII CATG 1 cut(s) 688
NlaIV GGNNCC 5 cut(s) 414, 516, 541, 602, 1084
NmeAIII GCCGAG 1 cut(s) 441
NmuCI GTSAC 1 cut(s) 1052
PaeR7I CTCGAG 1 cut(s) 397
PceI AGGCCT 1 cut(s) 1192
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PdiI GCCGGC 2 cut(s) 273, 519
PfeI GAWTC 1 cut(s) 972
PkrI GCNGC 9 cut(s) 7, 10, 13, 51, 303, 334, 580, 832, 1012
PleI GAGTC 3 cut(s) 167, 415, 1120
PpsI GAGTC 3 cut(s) 167, 415, 1120
Ppu21I YACGTR 1 cut(s) 511
PshAI GACNNNNGTC 1 cut(s) 917
PspFI CCCAGC 1 cut(s) 208
PspN4I GGNNCC 5 cut(s) 414, 516, 541, 602, 1084
PspPI GGNCC 2 cut(s) 435, 515
PspXI VCTCGAGB 1 cut(s) 397
PsuI RGATCY 2 cut(s) 706, 1082
RsaI GTAC 2 cut(s) 449, 461
RsaNI GTAC 2 cut(s) 448, 460
RseI CAYNNNNRTG 1 cut(s) 40
SaqAI TTAA 3 cut(s) 159, 711, 870
SatI GCNGC 9 cut(s) 6, 9, 12, 50, 302, 333, 579, 831, 1011
Sau3AI GATC 4 cut(s) 561, 610, 706, 1082
Sau96I GGNCC 2 cut(s) 435, 515
SchI GAGTC 3 cut(s) 167, 415, 1120
ScrFI CCNGG 4 cut(s) 426, 438, 544, 598
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 2 cut(s) 819, 844
Sfr274I CTCGAG 1 cut(s) 397
SlaI CTCGAG 1 cut(s) 397
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 3 cut(s) 397, 430, 659
SmoI CTYRAG 3 cut(s) 397, 430, 659
Sse9I AATT 6 cut(s) 98, 112, 140, 219, 319, 899
SseBI AGGCCT 1 cut(s) 1192
SspI AATATT 1 cut(s) 1002
SspMI CTAG 1 cut(s) 782
StuI AGGCCT 1 cut(s) 1192
StyD4I CCNGG 4 cut(s) 424, 436, 542, 596
TaiI ACGT 1 cut(s) 513
TaqI TCGA 5 cut(s) 198, 398, 548, 885, 1094
TaqII GACCGA 1 cut(s) 603
TasI AATT 6 cut(s) 98, 112, 140, 219, 319, 899
TauI GCSGC 3 cut(s) 8, 335, 581
TfiI GAWTC 1 cut(s) 972
Tru1I TTAA 3 cut(s) 159, 711, 870
Tru9I TTAA 3 cut(s) 159, 711, 870
TscAI CASTG 1 cut(s) 1054
TseFI GTSAC 1 cut(s) 1052
TseI GCWGC 6 cut(s) 8, 11, 49, 301, 830, 1010
Tsp45I GTSAC 1 cut(s) 1052
TspDTI ATGAA 3 cut(s) 269, 813, 1152
TspRI CASTG 1 cut(s) 1054
XapI RAATTY 3 cut(s) 98, 140, 899
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 397
XspI CTAG 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.