MD12G1236800.v1.1

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000368
Physical Location & Seq
Forward (+)
31054178 .. 31057644
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1236800.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGCGGCAACAGCTTCATTGCTCCAACCCAACCCACTGAGATGGAACAAGCGCAATCTCCTCCCCCCTCTCCGTACCCTCCCCGCAAACCCAATCAAATTCAAAAACGTCAATTTCCAAATCCAAAAACTCCCCTGCAAATTCAAAGTTCAATGCTTTAAGCAGTTACCCGAATCAAAATCTCGGTCCCTGGAAAACCCAGCACACCCAGCAACAAACCCACCAAACCCAATTGGGATTATCACTGGAAAGCTGCTGAATGCTCTGAAGGCGTTGAGGAAGCCGGCAATGGCCGCGGTGTTGTTTGGTCTGCTGCTGATGTCCGACCCGAATTTGGCATTGGCTGCATCGGGCGGGCGGGTCGGGGGAAGCTCGTTCTCGTCGCGCTCGTCGTCTTCCTCGTCGTCGTCGAGGAGTTATTCGGTGCCGAGGACTTCGAGTTCAAGGCCCGATTTTTCGTACTCCGCGCCGTACTACGCGCCTTCCCCGTTCGGGTTCGGTGGCGGGGGAGGTGGGTTTTACGTGGGGCCGGCAGTTGGGGTCGGAGTCGGAGCCGGGTCGAGCTTCTTCCTGATGTTGACGGGCTTCGCGGCGTTTGTTTTGGTTTCCGGGTTCCTTTCGGATCGGTCTGAGGGCAGTGTGCTTACTGATACAGAGAAAACAAGCGTTCTCAAGCTTCAGGTTGGTTTGTTGGGCATGGGACGAACACTCCAAAGGGATCTTAATCGGATTGCTGAAACTGCAGATACTTCTACCGCTGATGGTTTGGGCTATGTTTTGACAGAGACAACACTAGCTTTGCTACGGCATCCTGATTATTGCATCTCCGGCTATTCATCTGTTGCTCTAAAACGAGGCATAGAGGATGCAGAGAAACACTTTAATCAGCTTTCTATCGAAGAAAGGGGCAAATTTGATGAAGAGACACTTGTCAATGTTAATAACATAAGAAAGCAAAGCTCTACAAGCCGGAGTGCTAATGGATTCCGCAACGAATATATAGTGATAACAATATTGGTGGCTGCTGAAGGGGTGCATAAGTTGCCTGCCATCAATGGCAGTGGTGACTTAAAGGAAGCGTTGCAAAAGCTTGCCTCCATTCCGTCGAACAAAATAATGGCAGTAGAGGTCTTGTGGACTCCTCAGAATGAAACTGACACTCTGTCGGAGCGGGAACTACTTGAAGACTACCCGCTTTTGAGGCCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

404

Amino Acids

43.33

Weight (kDa)

9.44

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 221 - 403 8.2e-71 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 1171
AccII CGCG 5 cut(s) 296, 385, 467, 479, 590
AclWI GGATC 2 cut(s) 630, 726
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 4 cut(s) 98, 140, 331, 911
AcuI CTGAAG 3 cut(s) 287, 662, 1047
AfaI GTAC 3 cut(s) 76, 461, 473
AfiI CCNNNNNNNGG 3 cut(s) 334, 492, 536
AgsI TTSAA 5 cut(s) 103, 145, 152, 444, 1184
AhdI GACNNNNNGTC 1 cut(s) 1162
AjnI CCWGG 1 cut(s) 189
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 9 cut(s) 14, 253, 372, 564, 676, 797, 889, 960, 1090
AluI AGCT 9 cut(s) 14, 253, 372, 564, 676, 797, 889, 960, 1090
Alw26I GTCTC 2 cut(s) 779, 917
AlwI GGATC 2 cut(s) 630, 726
AoxI GGCC 4 cut(s) 291, 446, 527, 1202
ApeKI GCWGC 4 cut(s) 253, 313, 344, 1022
ApoI RAATTY 4 cut(s) 98, 140, 331, 911
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
AspLEI GCGC 4 cut(s) 54, 387, 469, 481
AspS9I GGNCC 3 cut(s) 186, 447, 527
AsuC2I CCSGG 2 cut(s) 556, 610
AsuHPI GGTGA 1 cut(s) 1076
AvaII GGWCC 1 cut(s) 186
BanI GGYRCC 1 cut(s) 424
BbsI GAAGAC 2 cut(s) 387, 1191
BbvI GCAGC 4 cut(s) 240, 300, 331, 1009
BccI CCATC 3 cut(s) 36, 755, 1058
BceAI ACGGC 2 cut(s) 454, 821
BciT130I CCWGG 1 cut(s) 191
BcnI CCSGG 2 cut(s) 556, 610
BcoDI GTCTC 2 cut(s) 779, 917
BfaI CTAG 1 cut(s) 794
BfmI CTRYAG 1 cut(s) 741
BisI GCNGC 7 cut(s) 6, 254, 294, 314, 345, 591, 1023
BlsI GCNGC 7 cut(s) 7, 255, 295, 315, 346, 592, 1024
Bme1390I CCNGG 3 cut(s) 191, 556, 610
Bme18I GGWCC 1 cut(s) 186
BmeRI GACNNNNNGTC 1 cut(s) 1162
BmgT120I GGNCC 3 cut(s) 186, 447, 527
BmiI GGNNCC 5 cut(s) 188, 426, 528, 553, 614
BmrFI CCNGG 3 cut(s) 191, 556, 610
BmsI GCATC 4 cut(s) 356, 817, 831, 856
BoxI GACNNNNGTC 1 cut(s) 929
BpiI GAAGAC 2 cut(s) 387, 1191
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 2 cut(s) 556, 610
BsaAI YACGTR 1 cut(s) 523
BsaBI GATNNNNATC 1 cut(s) 723
BsaJI CCNNGG 3 cut(s) 189, 294, 428
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 334, 492, 536
Bse118I RCCGGY 2 cut(s) 283, 529
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 2 cut(s) 17, 294
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 3 cut(s) 189, 294, 428
BseGI GGATG 2 cut(s) 808, 871
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 334, 492, 536
BseMI GCAATG 2 cut(s) 17, 294
BseMII CTCAG 3 cut(s) 29, 621, 1157
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 3 cut(s) 50, 427, 1131
BseXI GCAGC 4 cut(s) 240, 300, 331, 1009
BseYI CCCAGC 2 cut(s) 199, 208
Bsh1236I CGCG 5 cut(s) 296, 385, 467, 479, 590
BshFI GGCC 4 cut(s) 293, 448, 529, 1204
BshNI GGYRCC 1 cut(s) 424
BsiSI CCGG 6 cut(s) 284, 530, 555, 609, 828, 970
BslFI GGGAC 2 cut(s) 172, 714
BslI CCNNNNNNNGG 3 cut(s) 334, 492, 536
BsmAI GTCTC 2 cut(s) 779, 917
BsmFI GGGAC 2 cut(s) 172, 714
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 4 cut(s) 293, 448, 529, 1204
Bsp143I GATC 2 cut(s) 622, 718
BspANI GGCC 4 cut(s) 293, 448, 529, 1204
BspCNI CTCAG 3 cut(s) 30, 622, 1156
BspFNI CGCG 5 cut(s) 296, 385, 467, 479, 590
BspLI GGNNCC 5 cut(s) 188, 426, 528, 553, 614
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 2 cut(s) 630, 726
BspT107I GGYRCC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 1171
BsrDI GCAATG 2 cut(s) 17, 294
BsrFI RCCGGY 2 cut(s) 283, 529
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 2 cut(s) 283, 529
BssECI CCNNGG 3 cut(s) 189, 294, 428
BssMI GATC 2 cut(s) 622, 718
Bst2UI CCWGG 1 cut(s) 191
Bst6I CTCTTC 1 cut(s) 915
BstAPI GCANNNNNTGC 2 cut(s) 344, 1042
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 5 cut(s) 285, 356, 531, 1047, 1092
BstDEI CTNAG 3 cut(s) 38, 630, 1143
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 2 cut(s) 808, 871
BstFNI CGCG 5 cut(s) 296, 385, 467, 479, 590
BstHHI GCGC 4 cut(s) 54, 387, 469, 481
BstKTI GATC 2 cut(s) 625, 721
BstMAI GTCTC 2 cut(s) 779, 917
BstMBI GATC 2 cut(s) 622, 718
BstNI CCWGG 1 cut(s) 191
BstPAI GACNNNNGTC 1 cut(s) 929
BstSCI CCNGG 3 cut(s) 189, 554, 608
BstSFI CTRYAG 1 cut(s) 741
BstUI CGCG 5 cut(s) 296, 385, 467, 479, 590
BstV1I GCAGC 4 cut(s) 240, 300, 331, 1009
BstV2I GAAGAC 2 cut(s) 387, 1191
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 42
BstYI RGATCY 1 cut(s) 718
BsuRI GGCC 4 cut(s) 293, 448, 529, 1204
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 2 cut(s) 808, 871
BtsI GCAGTG 2 cut(s) 643, 1066
BtsIMutI CAGTG 4 cut(s) 35, 243, 643, 1066
Cac8I GCNNGC 5 cut(s) 285, 356, 531, 1047, 1092
CfoI GCGC 4 cut(s) 54, 387, 469, 481
Cfr10I RCCGGY 2 cut(s) 283, 529
Cfr13I GGNCC 3 cut(s) 186, 447, 527
Cfr42I CCGCGG 1 cut(s) 297
Csp6I GTAC 3 cut(s) 75, 460, 472
CspCI CAANNNNNGTGG 4 cut(s) 999, 1034, 1042, 1077
CviAII CATG 1 cut(s) 697
CviQI GTAC 3 cut(s) 75, 460, 472
DdeI CTNAG 3 cut(s) 38, 630, 1143
DpnI GATC 2 cut(s) 624, 720
DpnII GATC 2 cut(s) 622, 718
DriI GACNNNNNGTC 1 cut(s) 1162
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 915
Eam1105I GACNNNNNGTC 1 cut(s) 1162
EarI CTCTTC 1 cut(s) 915
Eco147I AGGCCT 1 cut(s) 1204
Eco47I GGWCC 1 cut(s) 186
Eco57I CTGAAG 3 cut(s) 287, 662, 1047
EcoRII CCWGG 1 cut(s) 189
FaeI CATG 1 cut(s) 700
FaiI YATR 8 cut(s) 698, 774, 860, 947, 999, 1001, 1038, 1210
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 2 cut(s) 172, 714
FatI CATG 1 cut(s) 696
FauI CCCGC 6 cut(s) 91, 347, 351, 497, 1164, 1200
Fnu4HI GCNGC 7 cut(s) 6, 254, 294, 314, 345, 591, 1023
FokI GGATG 2 cut(s) 795, 878
Fsp4HI GCNGC 7 cut(s) 6, 254, 294, 314, 345, 591, 1023
FspBI CTAG 1 cut(s) 794
GlaI GCGC 4 cut(s) 53, 386, 468, 480
GluI GCNGC 7 cut(s) 6, 254, 294, 314, 345, 591, 1023
GsaI CCCAGC 2 cut(s) 203, 212
HaeIII GGCC 4 cut(s) 293, 448, 529, 1204
HapII CCGG 6 cut(s) 284, 530, 555, 609, 828, 970
HhaI GCGC 4 cut(s) 54, 387, 469, 481
Hin1II CATG 1 cut(s) 700
Hin6I GCGC 4 cut(s) 52, 385, 467, 479
HinP1I GCGC 4 cut(s) 52, 385, 467, 479
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 2 cut(s) 674, 1088
HinfI GANTC 4 cut(s) 173, 546, 984, 1138
HpaII CCGG 6 cut(s) 284, 530, 555, 609, 828, 970
HphI GGTGA 1 cut(s) 1076
Hpy166II GTNNAC 2 cut(s) 579, 1137
Hpy188I TCNGA 9 cut(s) 267, 325, 545, 551, 622, 631, 729, 1146, 1168
Hpy188III TCNNGA 2 cut(s) 571, 812
Hpy8I GTNNAC 2 cut(s) 579, 1137
Hpy99I CGWCG 6 cut(s) 385, 394, 406, 409, 412, 1108
HpyAV CCTTC 3 cut(s) 262, 492, 1022
HpyCH4IV ACGT 2 cut(s) 108, 522
HpyCH4V TGCA 7 cut(s) 138, 347, 743, 822, 869, 1036, 1084
HpyF3I CTNAG 3 cut(s) 38, 630, 1143
HpySE526I ACGT 2 cut(s) 108, 522
Hsp92II CATG 1 cut(s) 700
HspAI GCGC 4 cut(s) 52, 385, 467, 479
KroI GCCGGC 2 cut(s) 283, 529
KroNI GCCGGC 2 cut(s) 285, 531
KspI CCGCGG 1 cut(s) 297
Kzo9I GATC 2 cut(s) 622, 718
LmnI GCTCC 3 cut(s) 27, 551, 1168
Lsp1109I GCAGC 4 cut(s) 240, 300, 331, 1009
LweI GCATC 4 cut(s) 356, 817, 831, 856
MaeI CTAG 1 cut(s) 794
MaeII ACGT 2 cut(s) 108, 522
MaeIII GTNAC 2 cut(s) 165, 1064
MalI GATC 2 cut(s) 624, 720
MbiI CCGCTC 1 cut(s) 1171
MboI GATC 2 cut(s) 622, 718
MboII GAAGA 5 cut(s) 387, 559, 911, 932, 1196
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 1 cut(s) 718
MluCI AATT 6 cut(s) 98, 112, 140, 231, 331, 911
MlyI GAGTC 2 cut(s) 555, 1132
MmeI TCCRAC 5 cut(s) 49, 348, 523, 529, 1146
MroNI GCCGGC 2 cut(s) 283, 529
MseI TTAA 5 cut(s) 159, 723, 882, 939, 1070
MslI CAYNNNNRTG 1 cut(s) 40
MspA1I CMGCKG 2 cut(s) 296, 758
MspI CCGG 6 cut(s) 284, 530, 555, 609, 828, 970
MspR9I CCNGG 3 cut(s) 191, 556, 610
MunI CAATTG 1 cut(s) 231
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 5 cut(s) 296, 385, 467, 479, 590
NaeI GCCGGC 2 cut(s) 285, 531
NciI CCSGG 2 cut(s) 556, 610
NdeII GATC 2 cut(s) 622, 718
NgoMIV GCCGGC 2 cut(s) 283, 529
NlaIII CATG 1 cut(s) 700
NlaIV GGNNCC 5 cut(s) 188, 426, 528, 553, 614
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 1 cut(s) 1064
PceI AGGCCT 1 cut(s) 1204
PcsI WCGNNNNNNNCGW 4 cut(s) 386, 389, 398, 407
PctI GAATGC 1 cut(s) 265
PdiI GCCGGC 2 cut(s) 285, 531
PfeI GAWTC 2 cut(s) 173, 984
PkrI GCNGC 7 cut(s) 7, 255, 295, 315, 346, 592, 1024
PleI GAGTC 2 cut(s) 554, 1132
PpsI GAGTC 2 cut(s) 554, 1132
Ppu21I YACGTR 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 929
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 2 cut(s) 199, 208
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 5 cut(s) 188, 426, 528, 553, 614
PspPI GGNCC 3 cut(s) 186, 447, 527
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 3 cut(s) 76, 461, 473
RsaNI GTAC 3 cut(s) 75, 460, 472
RseI CAYNNNNRTG 1 cut(s) 40
SacII CCGCGG 1 cut(s) 297
SaqAI TTAA 5 cut(s) 159, 723, 882, 939, 1070
SatI GCNGC 7 cut(s) 6, 254, 294, 314, 345, 591, 1023
Sau3AI GATC 2 cut(s) 622, 718
Sau96I GGNCC 3 cut(s) 186, 447, 527
SchI GAGTC 2 cut(s) 555, 1132
ScrFI CCNGG 3 cut(s) 191, 556, 610
SfaNI GCATC 4 cut(s) 356, 817, 831, 856
SfcI CTRYAG 1 cut(s) 741
Sfr303I CCGCGG 1 cut(s) 297
SgrBI CCGCGG 1 cut(s) 297
SinI GGWCC 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 6 cut(s) 98, 112, 140, 231, 331, 911
SseBI AGGCCT 1 cut(s) 1204
SspI AATATT 1 cut(s) 1014
SspMI CTAG 1 cut(s) 794
StuI AGGCCT 1 cut(s) 1204
StyD4I CCNGG 3 cut(s) 189, 554, 608
TaiI ACGT 2 cut(s) 111, 525
TaqI TCGA 5 cut(s) 410, 437, 560, 897, 1106
TaqII GACCGA 2 cut(s) 174, 615
TasI AATT 6 cut(s) 98, 112, 140, 231, 331, 911
TauI GCSGC 3 cut(s) 8, 296, 593
TfiI GAWTC 2 cut(s) 173, 984
Tru1I TTAA 5 cut(s) 159, 723, 882, 939, 1070
Tru9I TTAA 5 cut(s) 159, 723, 882, 939, 1070
TscAI CASTG 4 cut(s) 42, 250, 643, 1066
TseFI GTSAC 1 cut(s) 1064
TseI GCWGC 4 cut(s) 253, 313, 344, 1022
Tsp45I GTSAC 1 cut(s) 1064
TspDTI ATGAA 4 cut(s) 6, 825, 933, 1164
TspGWI ACGGA 2 cut(s) 62, 1092
TspRI CASTG 4 cut(s) 42, 250, 643, 1066
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 4 cut(s) 98, 140, 331, 911
XspI CTAG 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.