Rroxscaffold_164G00436120

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000164
OG0000368
Physical Location & Seq
Forward (+)
124059 .. 126880
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_164G00436120.1

Sequence Viewer

Length: 1398 bp
ATGGCTACATCTGCGTTGCTCGGAGCCAACTCCTTGATCTCATTGAAGCAAACCTCCCCAATCCCCTTCTCCCGTCTTCGTTCTCTCCCCACCAAGCCACTCCAGTTCACCTACTTCACCACAGCCACCTTGAAAACGCAACCCTTATCTTCTTCTTCTCCCAAATATGATCTGAAACTCAAATGCTCCTCGGAGAAGAATCACTTCACATCAGACGATGCCTCTCAGGCAGAAGTCAACCCAACTCAAGACCCACTTGAGACCATCGCCAATGAAATATTATCGAATGTTCTCAAGGGACTGAAAACACCTGCAGTAGAGAACCCACCATTTGATGCTATCTTCAATGAAACATTCTCGAACGCTCTCAAGGGACTCAAAACACCTGCAAAAGAGAATCCTAACCCACTTGAGGCTATCTTCAATGAAACATTAGCGAGAGCTCTCAAGGTACTCAAGACACCTGCCATGGCTGCCGTGTTGTTGAGTTTGTTATTGACGTTTAACCCGAACTGCTCGTGGGCCGCCTCGGGTGGTCGGGTCGGGGGAAGCTCGTTCTCCTCACGCTCTTCGTCGTCATCCTCGTCATGGTCGTCGTCTTATTCTTCTTCATCATCGTCCTATAGATCTTCAAGTTCTTCACCTTCGTATTCGTACACGTCGTATTCGTCATATTCAACGCCTTCATCATCGAGTTCTTCGTCTACTACCTCGATGTCCGAGGTCGTAATTTCTCTGTGCATAATCCTTCTGATTTTGGTTCTGTTGCCTGTCTCTTTGTCCTTTGCTGTAGTGGGCGCGGGCGCGGGCGTGGGCGCGGGCGCAAAGGCAAGTTTTGCTGCGAGCTCAAACGTTGTTAAGCTTCAGGTCGGTTTGTTGGGTACAGCTCGAGCACTCCAAAAAGATCTTGATCGTATCGCCGAAACTGCTAATACTTCTACCCGCAAGGGCTTGAGCTATGTCTTGACAGAGGCAACTGTAGCTTTACTTAGACATCCTGATTATTGGATCTCAAGTTATTCATCTGTTACTCCAAATCATAACATGGAGGATGCAGAGAAATGCTTCAATAAAAATTCTATTGAAGAGAGGGCTAAATTTGATGAAGAGACGCTTGTCAACGTGAACAACCAAAAAAGAAAAAGCTCGACAAGCAAGACTGATGATGGACTCCGAAATGAATATATAGTGGTGACATTGTTGGTTGCGATTGAGGGGAAGCATAAGTTGCCCACCATCAATAGCAGCGCTGACTTGAAGGAAGCATTGAAAAGCCTCGGATCCATTCCTTCAAAGAATATATTAGCAGCAGAAGTATTGTGGACTCCACAGAAAGAAAATGACACACTGTCCGAGGAGGAACTAATAGAAGATTACCCACTTCTGAAGCGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

465

Amino Acids

49.89

Weight (kDa)

8.44

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 284 - 465 6.4e-71 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 319, 394, 472
Acc36I ACCTGC 3 cut(s) 319, 394, 472
AccI GTMKAC 1 cut(s) 704
AccII CGCG 3 cut(s) 800, 806, 818
AciI CCGC 5 cut(s) 525, 800, 806, 818, 943
AclI AACGTT 1 cut(s) 852
AclWI GGATC 3 cut(s) 1016, 1275, 1288
AcsI RAATTY 2 cut(s) 1075, 1097
AcuI CTGAAG 1 cut(s) 848
AfaI GTAC 3 cut(s) 453, 656, 883
AfeI AGCGCT 1 cut(s) 1249
AfiI CCNNNNNNNGG 2 cut(s) 412, 588
AflIII ACRYGT 1 cut(s) 657
AhdI GACNNNNNGTC 1 cut(s) 1348
AjiI CACGTC 1 cut(s) 660
AluBI AGCT 8 cut(s) 443, 552, 846, 862, 887, 957, 983, 1146
AluI AGCT 8 cut(s) 443, 552, 846, 862, 887, 957, 983, 1146
Alw21I GWGCWC 3 cut(s) 445, 848, 895
Alw26I GTCTC 3 cut(s) 254, 778, 1103
AlwI GGATC 3 cut(s) 1016, 1275, 1288
Ama87I CYCGRG 2 cut(s) 529, 888
Aor51HI AGCGCT 1 cut(s) 1249
AoxI GGCC 1 cut(s) 522
ApeKI GCWGC 4 cut(s) 473, 839, 1245, 1307
ApoI RAATTY 2 cut(s) 1075, 1097
Asp700I GAANNNNTTC 2 cut(s) 203, 1064
AspLEI GCGC 5 cut(s) 800, 806, 818, 824, 1250
AspS9I GGNCC 1 cut(s) 522
AsuHPI GGTGA 4 cut(s) 100, 109, 633, 1204
AvaI CYCGRG 2 cut(s) 529, 888
BamHI GGATCC 1 cut(s) 1280
BanII GRGCYC 2 cut(s) 445, 848
BauI CACGAG 1 cut(s) 517
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 3 cut(s) 445, 848, 895
BbvI GCAGC 4 cut(s) 460, 826, 1257, 1319
BccI CCATC 3 cut(s) 272, 1160, 1244
BceAI ACGGC 1 cut(s) 461
BcoDI GTCTC 3 cut(s) 254, 778, 1103
BfmI CTRYAG 4 cut(s) 312, 622, 789, 978
BfoI RGCGCY 1 cut(s) 1251
BfuAI ACCTGC 3 cut(s) 319, 394, 472
BglI GCCNNNNNGGC 1 cut(s) 227
BglII AGATCT 2 cut(s) 626, 904
BisI GCNGC 5 cut(s) 474, 525, 840, 1246, 1308
BlsI GCNGC 5 cut(s) 475, 526, 841, 1247, 1309
BmeRI GACNNNNNGTC 1 cut(s) 1348
BmeT110I CYCGRG 2 cut(s) 529, 888
BmgBI CACGTC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 522
BmiI GGNNCC 2 cut(s) 25, 1282
BmsI GCATC 3 cut(s) 208, 325, 1042
BoxI GACNNNNGTC 1 cut(s) 1115
BpiI GAAGAC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 9 cut(s) 231, 278, 278, 353, 431, 431, 440, 973, 997
BsaBI GATNNNNATC 1 cut(s) 909
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 6 cut(s) 189, 468, 528, 720, 1276, 1353
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 588
Bse1I ACTGG 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 909
BseDI CCNNGG 6 cut(s) 189, 468, 528, 720, 1276, 1353
BseGI GGATG 3 cut(s) 578, 994, 1057
BseJI GATNNNNATC 1 cut(s) 909
BseLI CCNNNNNNNGG 2 cut(s) 412, 588
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 3 cut(s) 178, 550, 1370
BseXI GCAGC 4 cut(s) 460, 826, 1257, 1319
Bsh1236I CGCG 3 cut(s) 800, 806, 818
BshFI GGCC 1 cut(s) 524
BsiHKAI GWGCWC 3 cut(s) 445, 848, 895
BsiHKCI CYCGRG 2 cut(s) 529, 888
BslFI GGGAC 2 cut(s) 312, 387
BslI CCNNNNNNNGG 2 cut(s) 412, 588
BsmAI GTCTC 3 cut(s) 254, 778, 1103
BsmBI CGTCTC 1 cut(s) 1103
BsmFI GGGAC 2 cut(s) 312, 387
BsnI GGCC 1 cut(s) 524
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 2 cut(s) 529, 888
Bsp1286I GDGCHC 3 cut(s) 445, 848, 895
Bsp143I GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
Bsp19I CCATGG 1 cut(s) 468
BspACI CCGC 5 cut(s) 525, 800, 806, 818, 943
BspANI GGCC 1 cut(s) 524
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 3 cut(s) 800, 806, 818
BspLI GGNNCC 2 cut(s) 25, 1282
BspMAI CTGCAG 1 cut(s) 316
BspMI ACCTGC 3 cut(s) 319, 394, 472
BspPI GGATC 3 cut(s) 1016, 1275, 1288
BspQI GCTCTTC 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 254
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 6 cut(s) 189, 468, 528, 720, 1276, 1353
BssMI GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
BssSI CACGAG 1 cut(s) 517
BssT1I CCWWGG 1 cut(s) 468
Bst2BI CACGAG 1 cut(s) 517
Bst4CI ACNGT 2 cut(s) 979, 1350
Bst6I CTCTTC 3 cut(s) 574, 1080, 1101
BstAPI GCANNNNNTGC 2 cut(s) 836, 1228
BstC8I GCNNGC 4 cut(s) 802, 808, 820, 844
BstDEI CTNAG 2 cut(s) 225, 989
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 3 cut(s) 578, 994, 1057
BstFNI CGCG 3 cut(s) 800, 806, 818
BstH2I RGCGCY 1 cut(s) 1251
BstHHI GCGC 5 cut(s) 800, 806, 818, 824, 1250
BstKTI GATC 7 cut(s) 39, 172, 629, 907, 913, 1011, 1283
BstMAI GTCTC 3 cut(s) 254, 778, 1103
BstMBI GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
BstMWI GCNNNNNNNGC 8 cut(s) 11, 227, 473, 836, 926, 980, 1152, 1228
BstPAI GACNNNNGTC 1 cut(s) 1115
BstSFI CTRYAG 4 cut(s) 312, 622, 789, 978
BstUI CGCG 3 cut(s) 800, 806, 818
BstV1I GCAGC 4 cut(s) 460, 826, 1257, 1319
BstV2I GAAGAC 1 cut(s) 68
BstX2I RGATCY 4 cut(s) 626, 904, 1008, 1280
BstYI RGATCY 4 cut(s) 626, 904, 1008, 1280
BsuRI GGCC 1 cut(s) 524
BtgI CCRYGG 1 cut(s) 468
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 1 cut(s) 660
BtsCI GGATG 3 cut(s) 578, 994, 1057
BtsIMutI CAGTG 1 cut(s) 1346
BveI ACCTGC 3 cut(s) 319, 394, 472
Cac8I GCNNGC 4 cut(s) 802, 808, 820, 844
CfoI GCGC 5 cut(s) 800, 806, 818, 824, 1250
Cfr13I GGNCC 1 cut(s) 522
CseI GACGC 1 cut(s) 1120
Csp6I GTAC 3 cut(s) 452, 655, 882
CviAII CATG 3 cut(s) 469, 588, 1045
CviQI GTAC 3 cut(s) 452, 655, 882
DdeI CTNAG 2 cut(s) 225, 989
DpnI GATC 7 cut(s) 38, 171, 628, 906, 912, 1010, 1282
DpnII GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
DriI GACNNNNNGTC 1 cut(s) 1348
Eam1104I CTCTTC 3 cut(s) 574, 1080, 1101
Eam1105I GACNNNNNGTC 1 cut(s) 1348
EarI CTCTTC 3 cut(s) 574, 1080, 1101
Ecl136II GAGCTC 2 cut(s) 443, 846
Eco130I CCWWGG 1 cut(s) 468
Eco24I GRGCYC 2 cut(s) 445, 848
Eco31I GGTCTC 1 cut(s) 254
Eco47III AGCGCT 1 cut(s) 1249
Eco53kI GAGCTC 2 cut(s) 443, 846
Eco57I CTGAAG 1 cut(s) 848
Eco88I CYCGRG 2 cut(s) 529, 888
EcoICRI GAGCTC 2 cut(s) 443, 846
EcoT14I CCWWGG 1 cut(s) 468
EcoT38I GRGCYC 2 cut(s) 445, 848
ErhI CCWWGG 1 cut(s) 468
Esp3I CGTCTC 1 cut(s) 1103
FaeI CATG 3 cut(s) 472, 591, 1048
FalI AAGNNNNNCTT 6 cut(s) 188, 220, 240, 272, 1098, 1130
FaqI GGGAC 2 cut(s) 312, 387
FatI CATG 3 cut(s) 468, 587, 1044
FauI CCCGC 4 cut(s) 793, 799, 811, 950
FblI GTMKAC 1 cut(s) 704
Fnu4HI GCNGC 5 cut(s) 474, 525, 840, 1246, 1308
FokI GGATG 3 cut(s) 565, 981, 1064
FriOI GRGCYC 2 cut(s) 445, 848
Fsp4HI GCNGC 5 cut(s) 474, 525, 840, 1246, 1308
GlaI GCGC 5 cut(s) 799, 805, 817, 823, 1249
GluI GCNGC 5 cut(s) 474, 525, 840, 1246, 1308
GsuI CTGGAG 1 cut(s) 86
HaeII RGCGCY 1 cut(s) 1251
HaeIII GGCC 1 cut(s) 524
HgaI GACGC 1 cut(s) 1120
HhaI GCGC 5 cut(s) 800, 806, 818, 824, 1250
Hin1II CATG 3 cut(s) 472, 591, 1048
Hin6I GCGC 5 cut(s) 798, 804, 816, 822, 1248
HinP1I GCGC 5 cut(s) 798, 804, 816, 822, 1248
HincII GTYRAC 2 cut(s) 238, 1120
HindII GTYRAC 2 cut(s) 238, 1120
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 199, 375, 397, 1170, 1324
HphI GGTGA 4 cut(s) 100, 109, 633, 1204
Hpy166II GTNNAC 7 cut(s) 108, 238, 657, 705, 1120, 1126, 1323
Hpy188III TCNNGA 6 cut(s) 248, 358, 457, 908, 964, 998
Hpy8I GTNNAC 7 cut(s) 108, 238, 657, 705, 1120, 1126, 1323
Hpy99I CGWCG 3 cut(s) 577, 598, 664
HpyAV CCTTC 6 cut(s) 76, 654, 693, 758, 1252, 1299
HpyCH4III ACNGT 2 cut(s) 979, 1350
HpyCH4IV ACGT 4 cut(s) 500, 659, 852, 1122
HpyCH4V TGCA 4 cut(s) 314, 389, 741, 1055
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 227, 473, 836, 926, 980, 1152, 1228
HpyF3I CTNAG 2 cut(s) 225, 989
HpySE526I ACGT 4 cut(s) 500, 659, 852, 1122
Hsp92II CATG 3 cut(s) 472, 591, 1048
HspAI GCGC 5 cut(s) 798, 804, 816, 822, 1248
Kzo9I GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
LguI GCTCTTC 1 cut(s) 574
LmnI GCTCC 2 cut(s) 23, 191
LpnPI CCDG 8 cut(s) 116, 212, 324, 399, 477, 783, 851, 1011
Lsp1109I GCAGC 4 cut(s) 460, 826, 1257, 1319
LweI GCATC 3 cut(s) 208, 325, 1042
MaeII ACGT 4 cut(s) 500, 659, 852, 1122
MaeIII GTNAC 2 cut(s) 1027, 1192
MalI GATC 7 cut(s) 38, 171, 628, 906, 912, 1010, 1282
MboI GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
MflI RGATCY 4 cut(s) 626, 904, 1008, 1280
MhlI GDGCHC 3 cut(s) 445, 848, 895
MluCI AATT 3 cut(s) 729, 1075, 1097
MlyI GAGTC 3 cut(s) 369, 1164, 1318
MroXI GAANNNNTTC 2 cut(s) 203, 1064
MseI TTAA 2 cut(s) 504, 858
MvnI CGCG 3 cut(s) 800, 806, 818
MwoI GCNNNNNNNGC 8 cut(s) 11, 227, 473, 836, 926, 980, 1152, 1228
NcoI CCATGG 1 cut(s) 468
NdeII GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
NlaIII CATG 3 cut(s) 472, 591, 1048
NlaIV GGNNCC 2 cut(s) 25, 1282
NmuCI GTSAC 1 cut(s) 1192
PaeR7I CTCGAG 1 cut(s) 888
PaqCI CACCTGC 3 cut(s) 319, 394, 472
PciSI GCTCTTC 1 cut(s) 574
PcsI WCGNNNNNNNCGW 6 cut(s) 506, 581, 590, 659, 665, 698
PdmI GAANNNNTTC 2 cut(s) 203, 1064
PfeI GAWTC 2 cut(s) 199, 397
PkrI GCNGC 5 cut(s) 475, 526, 841, 1247, 1309
PleI GAGTC 3 cut(s) 369, 1164, 1318
PpsI GAGTC 3 cut(s) 369, 1164, 1318
PshAI GACNNNNGTC 1 cut(s) 1115
Psp124BI GAGCTC 2 cut(s) 445, 848
Psp1406I AACGTT 1 cut(s) 852
PspN4I GGNNCC 2 cut(s) 25, 1282
PspPI GGNCC 1 cut(s) 522
PspXI VCTCGAGB 1 cut(s) 888
PstI CTGCAG 1 cut(s) 316
PsuI RGATCY 4 cut(s) 626, 904, 1008, 1280
RsaI GTAC 3 cut(s) 453, 656, 883
RsaNI GTAC 3 cut(s) 452, 655, 882
SacI GAGCTC 2 cut(s) 445, 848
SapI GCTCTTC 1 cut(s) 574
SaqAI TTAA 2 cut(s) 504, 858
SatI GCNGC 5 cut(s) 474, 525, 840, 1246, 1308
Sau3AI GATC 7 cut(s) 36, 169, 626, 904, 910, 1008, 1280
Sau96I GGNCC 1 cut(s) 522
SchI GAGTC 3 cut(s) 369, 1164, 1318
SduI GDGCHC 3 cut(s) 445, 848, 895
SfaNI GCATC 3 cut(s) 208, 325, 1042
SfcI CTRYAG 4 cut(s) 312, 622, 789, 978
Sfr274I CTCGAG 1 cut(s) 888
SlaI CTCGAG 1 cut(s) 888
Sse9I AATT 3 cut(s) 729, 1075, 1097
SsiI CCGC 5 cut(s) 525, 800, 806, 818, 943
SspI AATATT 1 cut(s) 279
SstI GAGCTC 2 cut(s) 445, 848
StyI CCWWGG 1 cut(s) 468
TaaI ACNGT 2 cut(s) 979, 1350
TaiI ACGT 4 cut(s) 503, 662, 855, 1125
TaqI TCGA 6 cut(s) 284, 359, 692, 713, 889, 1148
TasI AATT 3 cut(s) 729, 1075, 1097
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 2 cut(s) 199, 397
Tru1I TTAA 2 cut(s) 504, 858
Tru9I TTAA 2 cut(s) 504, 858
TscAI CASTG 1 cut(s) 1353
TseFI GTSAC 1 cut(s) 1192
TseI GCWGC 4 cut(s) 473, 839, 1245, 1307
Tsp45I GTSAC 1 cut(s) 1192
TspDTI ATGAA 8 cut(s) 288, 363, 441, 600, 675, 1011, 1119, 1194
TspRI CASTG 1 cut(s) 1353
XapI RAATTY 2 cut(s) 1075, 1097
XhoI CTCGAG 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 704
XmnI GAANNNNTTC 2 cut(s) 203, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.