AT1G54520

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000368
Physical Location & Seq
Forward (+)
20363393 .. 20366123
2731 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54520.1

Sequence Viewer

Length: 1176 bp
ATGGCGTCTTCTTCCACCTTTCTGGAATTAACTCCATTTCAATGGAATCAACCTCTCCCATACACACAACGACCCCATCATCGTACTGTTCTTCTCTACTCCAAACCTCAAAGGAGATCAAATTCGATTCGTCTCCAAATCTCCGTCAAATACAAGCAATCAACATCTTCTTCAGATCCAGATTTGAGATCCAATTTCAATCCATTTGAACAAATCGCGATTCAGGTCAAAAAAGCGTTAGATTCGTTGAAGAAACCTGCTATAGCTGCGGTTTTACTTGGTTTGCTTTTGTTCTATGATCCTAATTCGGCTTTAGCTGCGTCTGGTGGTAGAATTGGTGGTAATTCCTTCTCGTCGAGGTCTCGGAGCTCTTCTTCTTCTTCTTCGCAATCGTATTCTGTGCCGAGAACATCGAATCCGAGCTTTTCGTACTCTGCTAGGACCGCTCCGTACTATGGACCGTCACCTTTCGGTGGGGGTTTTGTGGGCCCGGCGGTTGGTTTTGGGTTTGGTGGTTTTTCGAGCTTCTCCTTGATTTTGGTTGGTTTCGCTGCGTTTGTTTTGGTCTCTGGATTCCTCTCAGATCGGTCACAGGATGATAGCATTTTAACAGATACTCAGAAAACTAGCGTCATTAAGCTACAGGTGGGGTTGCTTGGTTTGGGGAGGACACTACAACAAGATTTTAATCGTCTTGCTGAAAGTTCGGATACATCCACACCGGAGGGTCTGAGCTATGTTTTAACCGAGGCAACATTAGCTTTGCTGAGACACCCTGATTACTGCATCTCTTGTTATTCATCAGTGGATGTGAAGCCGAGTATAGAAAAGGGAGAGAAGCGATTTAATCAACTCTCCATTGAAGAACGAGGAAAATTTGATGAGGAGACACTTGTTAACGTGAATAGCATAAAGAGACAAAGCTCGAAGATTCGGAAAGCTAGCGGTTTCAGCAATGAATATATTGTGGTAACCATCTTGATGGCTGCAGAGGGTATACATAAACTGCCACCAATAAACGGAACTACAGATCTGAAGGAAGCCTTACTGAAACTCGGGTCAATACCAAGAAACAAAATAATGGCAGTAGAAGTACTATGGACACCGCAGAACGAAGCCGACGCATTGTCAGAAAGGGAACTACTTGAAGATTACCCACTTTTGAGGCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

391

Amino Acids

42.84

Weight (kDa)

9.22

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 209 - 391 1.4e-64 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54520
fragaria_vesca FvH4_4g09430 FvH4_5g29750 FvH4_6g03490 FvH4_6g03500 FvH4_6g03510 FvH4_6g03510 FvH4_6g03510 FvH4_6g03520 FvH4_6g03530 FvH4_6g03530 FvH4_6g03530
malus_domestica MD04G1220400.v1.1 MD12G1236800.v1.1
prunus_persica Prupe.6G339400_v2.0.a1 Prupe.6G339400_v2.0.a1
pyrus_communis pycom04g19510 pycom12g21790
rosa_chinensis RchiOBHm_Chr3g0451981 RchiOBHm_Chr3g0451991 RchiOBHm_Chr3g0452001 RchiOBHm_Chr3g0452011 RchiOBHm_Chr3g0452041 RchiOBHm_Chr3g0452111 RchiOBHm_Chr3g0452121
rosa_laevigata RLG00000009056 RLG00000025627 RLG00000025628 RLG00000025630 RLG00000025631 RLG00000025632 RLG00000035582
rosa_multiflora Rmu_co8213316.1_g000001 Rmu_co8226081.1_g000001 Rmu_co8290691.1_g000001 Rmu_sc0001358.1_g000002 Rmu_sc0001358.1_g000004 Rmu_sc0001358.1_g000008 Rmu_sc0001358.1_g000011 Rmu_sc0001448.1_g000001 Rmu_sc0001448.1_g000005 Rmu_sc0001448.1_g000009 Rmu_sc0001729.1_g000006 Rmu_sc0001729.1_g000012 Rmu_sc0001729.1_g000017
rosa_roxburghii Rroxscaffold_164G00436060 Rroxscaffold_164G00436070 Rroxscaffold_164G00436100 Rroxscaffold_164G00436110 Rroxscaffold_164G00436120 Rroxscaffold_164G00436130 Rroxscaffold_4G00296230 Rroxscaffold_6G00389530 Rroxscaffold_6G00429160 Rroxscaffold_6G00429170 Rroxscaffold_6G00429200 Rroxscaffold_6G00429220 Rroxscaffold_6G00429240
rosa_rugosa Rorug01G0052400 Rorug01G0052500 Rorug02G0637900 Rorug02G0637900 Rorug02G0638000 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638100 Rorug02G0638200 Rorug02G0638300 Rorug02G0638400 Rorug04G0037400
rosa_samantha Rh2DG349300 Rh3AG039600 Rh3AG039700 Rh3AG039800 Rh3AG039900 Rh3AG040200 Rh3AG040300 Rh3BG041100 Rh3BG041200 Rh3BG041300 Rh3BG041500 Rh3BG041700 Rh3BG041800 Rh3CG039500 Rh3CG039600 Rh3CG039700 Rh3CG039800 Rh3CG040000 Rh3CG040200 Rh3DG040200 Rh3DG040400 Rh3DG040500 Rh3DG041000 Rh3DG041100 Rh4BG107500 Rh6BG085400 Rh6DG400800
rosa_wichuraiana Rw0G023520 Rw3G002980 Rw3G002990 Rw3G003010 Rw3G003030 Rw3G003040 Rw3G003060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 265
AccBSI CCGCTC 1 cut(s) 446
AccI GTMKAC 1 cut(s) 997
AccII CGCG 1 cut(s) 218
AciI CCGC 5 cut(s) 269, 444, 494, 945, 1106
AclWI GGATC 3 cut(s) 170, 183, 293
AcsI RAATTY 2 cut(s) 121, 875
AcuI CTGAAG 2 cut(s) 156, 1055
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 85, 431, 452, 1095
AfiI CCNNNNNNNGG 4 cut(s) 455, 473, 497, 1019
AgsI TTSAA 6 cut(s) 41, 199, 209, 250, 863, 1148
AhdI GACNNNNNGTC 1 cut(s) 1126
AloI GAACNNNNNNTCC 2 cut(s) 400, 432
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 6 cut(s) 137, 366, 571, 763, 881, 910
AlwI GGATC 3 cut(s) 170, 183, 293
Ama87I CYCGRG 1 cut(s) 1055
AoxI GGCC 2 cut(s) 487, 1166
ApaI GGGCCC 1 cut(s) 491
ApeKI GCWGC 4 cut(s) 266, 317, 551, 986
ApoI RAATTY 2 cut(s) 121, 875
AspS9I GGNCC 4 cut(s) 441, 458, 487, 488
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 456
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 1 cut(s) 1055
AvaII GGWCC 2 cut(s) 441, 458
BaeGI GKGCMC 1 cut(s) 491
BanII GRGCYC 2 cut(s) 371, 491
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 4 cut(s) 253, 304, 538, 973
BccI CCATC 3 cut(s) 84, 976, 983
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 1 cut(s) 491
BcoDI GTCTC 6 cut(s) 137, 366, 571, 763, 881, 910
BfaI CTAG 3 cut(s) 438, 627, 942
BfmI CTRYAG 4 cut(s) 261, 641, 987, 1026
BfuAI ACCTGC 1 cut(s) 265
BfuI GTATCC 1 cut(s) 703
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 4 cut(s) 267, 318, 552, 987
BlsI GCNGC 4 cut(s) 268, 319, 553, 988
BmcAI AGTACT 1 cut(s) 1095
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 441, 458
BmeRI GACNNNNNGTC 1 cut(s) 1126
BmeT110I CYCGRG 1 cut(s) 1055
BmgT120I GGNCC 4 cut(s) 441, 458, 487, 488
BmiI GGNNCC 1 cut(s) 489
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 795
BmtI GCTAGC 1 cut(s) 945
BpuMI CCSGG 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 687
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 366, 571
BsaJI CCNNGG 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 4 cut(s) 455, 473, 497, 1019
Bse3DI GCAATG 1 cut(s) 961
Bse8I GATNNNNATC 1 cut(s) 687
BseDI CCNNGG 1 cut(s) 747
BseGI GGATG 3 cut(s) 601, 713, 814
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 4 cut(s) 455, 473, 497, 1019
BseMI GCAATG 1 cut(s) 961
BseMII CTCAG 4 cut(s) 594, 632, 722, 758
BseRI GAGGAG 1 cut(s) 899
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 4 cut(s) 253, 304, 538, 973
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 2 cut(s) 489, 1168
BsiHKAI GWGCWC 1 cut(s) 371
BsiHKCI CYCGRG 1 cut(s) 1055
BsiSI CCGG 2 cut(s) 491, 722
BslI CCNNNNNNNGG 4 cut(s) 455, 473, 497, 1019
BsmAI GTCTC 6 cut(s) 137, 366, 571, 763, 881, 910
BsmBI CGTCTC 1 cut(s) 137
BsnI GGCC 2 cut(s) 489, 1168
Bso31I GGTCTC 2 cut(s) 366, 571
BsoBI CYCGRG 1 cut(s) 1055
Bsp120I GGGCCC 1 cut(s) 487
Bsp1286I GDGCHC 2 cut(s) 371, 491
Bsp143I GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
Bsp68I TCGCGA 1 cut(s) 218
BspACI CCGC 5 cut(s) 269, 444, 494, 945, 1106
BspANI GGCC 2 cut(s) 489, 1168
BspCNI CTCAG 4 cut(s) 593, 631, 723, 759
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 1 cut(s) 489
BspMAI CTGCAG 1 cut(s) 991
BspMI ACCTGC 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 3 cut(s) 170, 183, 293
BspQI GCTCTTC 1 cut(s) 376
BspTNI GGTCTC 2 cut(s) 366, 571
BsrBI CCGCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 961
BssECI CCNNGG 1 cut(s) 747
BssMI GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
BssNAI GTATAC 1 cut(s) 998
BssNI GRCGYC 1 cut(s) 5
Bst1107I GTATAC 1 cut(s) 998
Bst4CI ACNGT 2 cut(s) 88, 462
Bst6I CTCTTC 1 cut(s) 376
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 943
BstDEI CTNAG 4 cut(s) 580, 618, 731, 767
BstEII GGTNACC 1 cut(s) 970
BstF5I GGATG 3 cut(s) 601, 713, 814
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 6 cut(s) 119, 178, 191, 301, 586, 1033
BstMAI GTCTC 6 cut(s) 137, 366, 571, 763, 881, 910
BstMBI GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
BstMWI GCNNNNNNNGC 5 cut(s) 266, 317, 443, 758, 951
BstPI GGTNACC 1 cut(s) 970
BstSCI CCNGG 1 cut(s) 489
BstSFI CTRYAG 4 cut(s) 261, 641, 987, 1026
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 4 cut(s) 253, 304, 538, 973
BstX2I RGATCY 3 cut(s) 175, 188, 1030
BstXI CCANNNNNNTGG 3 cut(s) 22, 42, 982
BstYI RGATCY 3 cut(s) 175, 188, 1030
BstZ17I GTATAC 1 cut(s) 998
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 2 cut(s) 489, 1168
BtsCI GGATG 3 cut(s) 601, 713, 814
BtsIMutI CAGTG 1 cut(s) 810
BtuMI TCGCGA 1 cut(s) 218
BveI ACCTGC 1 cut(s) 265
Cac8I GCNNGC 1 cut(s) 943
Cfr13I GGNCC 4 cut(s) 441, 458, 487, 488
CseI GACGC 3 cut(s) 309, 619, 1130
Csp6I GTAC 4 cut(s) 84, 430, 451, 1094
CviQI GTAC 4 cut(s) 84, 430, 451, 1094
DdeI CTNAG 4 cut(s) 580, 618, 731, 767
DpnI GATC 6 cut(s) 118, 177, 190, 300, 585, 1032
DpnII GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
DriI GACNNNNNGTC 1 cut(s) 1126
Eam1104I CTCTTC 1 cut(s) 376
Eam1105I GACNNNNNGTC 1 cut(s) 1126
EarI CTCTTC 1 cut(s) 376
Ecl136II GAGCTC 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 1168
Eco24I GRGCYC 2 cut(s) 371, 491
Eco31I GGTCTC 2 cut(s) 366, 571
Eco47I GGWCC 2 cut(s) 441, 458
Eco53kI GAGCTC 1 cut(s) 369
Eco57I CTGAAG 2 cut(s) 156, 1055
Eco88I CYCGRG 1 cut(s) 1055
Eco91I GGTNACC 1 cut(s) 970
EcoICRI GAGCTC 1 cut(s) 369
EcoO65I GGTNACC 1 cut(s) 970
EcoT38I GRGCYC 2 cut(s) 371, 491
Esp3I CGTCTC 1 cut(s) 137
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FblI GTMKAC 1 cut(s) 997
Fnu4HI GCNGC 4 cut(s) 267, 318, 552, 987
FokI GGATG 3 cut(s) 608, 700, 821
FriOI GRGCYC 2 cut(s) 371, 491
Fsp4HI GCNGC 4 cut(s) 267, 318, 552, 987
FspBI CTAG 3 cut(s) 438, 627, 942
GluI GCNGC 4 cut(s) 267, 318, 552, 987
HaeIII GGCC 2 cut(s) 489, 1168
HapII CCGG 2 cut(s) 491, 722
HgaI GACGC 3 cut(s) 309, 619, 1130
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 898
HindII GTYRAC 1 cut(s) 898
HinfI GANTC 7 cut(s) 46, 127, 220, 242, 415, 573, 931
HpaI GTTAAC 1 cut(s) 898
HpaII CCGG 2 cut(s) 491, 722
HphI GGTGA 1 cut(s) 456
Hpy166II GTNNAC 2 cut(s) 898, 998
Hpy188III TCNNGA 5 cut(s) 23, 179, 217, 570, 979
Hpy8I GTNNAC 2 cut(s) 898, 998
Hpy99I CGWCG 2 cut(s) 358, 1124
HpyAV CCTTC 2 cut(s) 358, 1030
HpyCH4III ACNGT 2 cut(s) 88, 462
HpyCH4IV ACGT 1 cut(s) 900
HpyCH4V TGCA 2 cut(s) 786, 989
HpyF10VI GCNNNNNNNGC 5 cut(s) 266, 317, 443, 758, 951
HpyF3I CTNAG 4 cut(s) 580, 618, 731, 767
HpySE526I ACGT 1 cut(s) 900
Hsp92I GRCGYC 1 cut(s) 5
KspAI GTTAAC 1 cut(s) 898
Kzo9I GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
LguI GCTCTTC 1 cut(s) 376
LmnI GCTCC 2 cut(s) 366, 451
Lsp1109I GCAGC 4 cut(s) 253, 304, 538, 973
LweI GCATC 1 cut(s) 795
MaeI CTAG 3 cut(s) 438, 627, 942
MaeII ACGT 1 cut(s) 900
MaeIII GTNAC 3 cut(s) 462, 588, 970
MalI GATC 6 cut(s) 118, 177, 190, 300, 585, 1032
MbiI CCGCTC 1 cut(s) 446
MboI GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
MflI RGATCY 3 cut(s) 175, 188, 1030
MhlI GDGCHC 2 cut(s) 371, 491
MluCI AATT 7 cut(s) 26, 121, 193, 304, 333, 343, 875
MseI TTAA 7 cut(s) 29, 608, 636, 687, 743, 846, 897
MslI CAYNNNNRTG 2 cut(s) 40, 980
MspI CCGG 2 cut(s) 491, 722
MspR9I CCNGG 1 cut(s) 491
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 5 cut(s) 266, 317, 443, 758, 951
NciI CCSGG 1 cut(s) 491
NdeII GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
NheI GCTAGC 1 cut(s) 941
NlaIV GGNNCC 1 cut(s) 489
NmeAIII GCCGAG 2 cut(s) 429, 843
NmuCI GTSAC 2 cut(s) 462, 588
NruI TCGCGA 1 cut(s) 218
PceI AGGCCT 1 cut(s) 1168
PciSI GCTCTTC 1 cut(s) 376
PfeI GAWTC 7 cut(s) 46, 127, 220, 242, 415, 573, 931
PkrI GCNGC 4 cut(s) 268, 319, 553, 988
Psp124BI GAGCTC 1 cut(s) 371
PspEI GGTNACC 1 cut(s) 970
PspN4I GGNNCC 1 cut(s) 489
PspOMI GGGCCC 1 cut(s) 487
PspPI GGNCC 4 cut(s) 441, 458, 487, 488
PstI CTGCAG 1 cut(s) 991
PsuI RGATCY 3 cut(s) 175, 188, 1030
RruI TCGCGA 1 cut(s) 218
RsaI GTAC 4 cut(s) 85, 431, 452, 1095
RsaNI GTAC 4 cut(s) 84, 430, 451, 1094
RseI CAYNNNNRTG 2 cut(s) 40, 980
SacI GAGCTC 1 cut(s) 371
SapI GCTCTTC 1 cut(s) 376
SaqAI TTAA 7 cut(s) 29, 608, 636, 687, 743, 846, 897
SatI GCNGC 4 cut(s) 267, 318, 552, 987
Sau3AI GATC 6 cut(s) 116, 175, 188, 298, 583, 1030
Sau96I GGNCC 4 cut(s) 441, 458, 487, 488
ScaI AGTACT 1 cut(s) 1095
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 2 cut(s) 371, 491
SfaNI GCATC 1 cut(s) 795
SfcI CTRYAG 4 cut(s) 261, 641, 987, 1026
SinI GGWCC 2 cut(s) 441, 458
SmiMI CAYNNNNRTG 2 cut(s) 40, 980
Sse9I AATT 7 cut(s) 26, 121, 193, 304, 333, 343, 875
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 5 cut(s) 269, 444, 494, 945, 1106
SspMI CTAG 3 cut(s) 438, 627, 942
SstI GAGCTC 1 cut(s) 371
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 1 cut(s) 489
TaaI ACNGT 2 cut(s) 88, 462
TaiI ACGT 1 cut(s) 903
TaqI TCGA 5 cut(s) 125, 356, 413, 521, 926
TaqII GACCGA 1 cut(s) 576
TasI AATT 7 cut(s) 26, 121, 193, 304, 333, 343, 875
TatI WGTACW 1 cut(s) 1093
TfiI GAWTC 7 cut(s) 46, 127, 220, 242, 415, 573, 931
Tru1I TTAA 7 cut(s) 29, 608, 636, 687, 743, 846, 897
Tru9I TTAA 7 cut(s) 29, 608, 636, 687, 743, 846, 897
TscAI CASTG 1 cut(s) 810
TseFI GTSAC 2 cut(s) 462, 588
TseI GCWGC 4 cut(s) 266, 317, 551, 986
Tsp45I GTSAC 2 cut(s) 462, 588
TspDTI ATGAA 2 cut(s) 789, 972
TspGWI ACGGA 3 cut(s) 133, 438, 1035
TspRI CASTG 1 cut(s) 810
VpaK11BI GGWCC 2 cut(s) 441, 458
XapI RAATTY 2 cut(s) 121, 875
XmiI GTMKAC 1 cut(s) 997
XspI CTAG 3 cut(s) 438, 627, 942
ZrmI AGTACT 1 cut(s) 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.