Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001186

Physical Location & Seq

Genomic Coordinates Forward (+)

16574283 .. 16578057

Gene Structure 3775 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1192500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGAATGGTGGTACAGCTTCTGAAGAAGAATTCTCTGAGTTTGTAGAGGTTGATCCTACTGGAAGATATGGAAGATACAACGAAGTTCTTGGCAAAGGAGCTTCAAAAACAGTATATAGGGCCTTTGATGAGTATGAAGGGATTGAAGTGGCATGGAATCAGGTGAAGCTTTATGATTTTCTGAGAAACCCTGAGGATCTCGAGAGGCTATACCGCGAAATTCATCTACTCAAGACCTTGAAACACAACAATATCATGAAGTTTTACACCTCTTGGGTTGATACTACCAACAGAAATATCAACTTTGTTACTGAGATGTTTACTTCTGGTACTCTCAGGCAGTATAGGCTAAAACACAAGAGAGTTAATATCAGGGCAGTGAAGCATTGGTGCAGGCAGATTTTGAAAGGGCTTCTCTATCTTCACAGCCATGACCCTCCTGTGATCCACAGAGATCTCAAGTGTGACAACATTTTCATCAATGGGAACCAAGGTGAGGTCAAGATTGGTGATCTTGGCCTCGCTGCTGTCCTCCGTAAATCTCACGCTGATCGTTGTGTTGGAACACCGGAGTTCATGGCTCCAGAGGTTTACGAAGAGGAATACAATGAGTTGGTTGACATTTATTCTTTTGGGATGTGCATCTTGGAGATGGTTACCTTTGAATATCCATATAGCGAATGCAATAATCCTGCTCAAATCTATAAGAAAGTTATCACTGGGAAAAAGCCAGAAGCCCTATTCAAGGTGAAGGATCCTGAGGTGCGGCGTTTTGTTGAGAAGTGCTTAGTGACCGTGTCTTGTAGGCTTTCTGCTAGGGAGCTTTTGAGAGACCCCTTTCTCCAGGTTGATGACTACGGATACAATTTAAGGCCAACAGAGGATCAAAGGGATATGTATGAAATAGGGCCTCTCTTAAGACAACCTCACTCTTCACTTCATCAAAGTAACAGCTCTTTGAGCAACGGGCATGGCAATTATCTTGGTTACGAAACAGAAAATGATTTGGACTACAATCCGATTGATTATGGATTGAGCGAAATTGATCTCTTCGCTTGTCAAGAGGATGAGCATTTTGGAGATGTTGACCTCACAATCAAAGGGAGGCGGAGAGAAGACGATGACATCTTTTTAAGGCTCAGAATTGCAGATAAAGAAGGCCGCATCCGAAACATCTATTTCCCTTTTGATACTGAGAATGACACGGCATTGAGTGTTGCCAGAGAGATGGTTTCTGAGCTTGATATCACAGACCAAGATGTTACAAAAATAGCAGAGATGATTGATGGTGAAATTACTACCTTGGTACCGGAGTGGATTAAGGGACGTGGCATAGAAGAAATTCCCCATTGCAATAATGCAAGTTTTTGTCAAAATTGTGCTTCGTATGGTTCCATTTTGGATTATGCATCACCGGATATTCCTGGTGTCAAGCACCTCCAAGTTCTTCAGTGCTCAAAACACGGATCAGCTGCTGTCCATGGACGTTTCGAAGAGATTACATACCAAGTTGAAGGCGCAGAGCGTAGTCTTACAGGTGCACCTAGAACATCAAACCATTCTGATGGCTTGCACTATGCTGATATCTGGGCTCAAAGAGAAGGACCGGAATTAAGCTCACAAGGTTCAGGAGATTCGAGTGATATCCAATGCAACGAAAATCATGAAACATTGGATGTAACAAACTTTGGAAAGGAAGAGAGAATTATAATGATGGACAATCAGAGTGATTCGAAAGCAAGAAATTCTATCTCAGAGAAACTTTCAGCCACTTCTGATCGGTTAGATGATTATGAGAATGAGCTTCGCCAAGAACTGCGATGGCTCAAAGCAACGTATCAAATGCAGTTGAGGGAGATTAGGGACCAACAGCTTGGAGCTAGACCGAAAGCTTCAAGCCTAATTCCAAATTCAAATCACTCAGAGAACAGGGAGACAAATGGAGCTTCAATGTCCTTGGCTTCGCCCCAGCGGACAAAAGAAAACAAAAAACCTTCCTTGAAATCCTTTTCCTCGGGGAAGCATTTCACATATTTTCCAGTGGATGCTGAAAAGAAACATTTGGCAAGCCCTGGTGTCCAAAATCACGAGGCATTTGATGGGGCTTACAGTCCTGAACAAATCATTACTGCAAAGAGTTTCTATATTGGTGCATTGCTTCCACACTCTCTGCACCGGGCAACTTCTCTACCGGTCGATGCTATAGATGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0009987 GO:0010228 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048511 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0061458 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

738

Amino Acids

84.1

Weight (kDa)

5.35

Isoelectric Point (pI)

39.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	27 - 281	2.7e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	27 - 263	7.9e-33	Protein tyrosine and serine/threonine kinase
OSR1_C	PF12202	377 - 431	7.7e-06	Oxidative-stress-responsive kinase 1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001186)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04910 AT3G04910 AT3G04910 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080 AT5G28080
fragaria_vesca	FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_4g15470 FvH4_6g03470 FvH4_6g03470
malus_domestica	MD04G1220500.v1.1 MD12G1236900.v1.1 MD13G1192500.v1.1 MD16G1193000.v1.1
prunus_persica	Prupe.1G013100_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1 Prupe.6G339500_v2.0.a1
pyrus_communis	pycom04g19520 pycom12g21800 pycom13g16490 pycom16g16300
rosa_chinensis	RchiOBHm_Chr3g0451971 RchiOBHm_Chr4g0416321
rosa_laevigata	RLG00000007995 RLG00000025633
rosa_multiflora	Rmu_sc0001145.1_g000025 Rmu_sc0006436.1_g000001
rosa_roxburghii	Rroxscaffold_164G00436150 Rroxscaffold_5G00360170 Rroxscaffold_6G00429140
rosa_rugosa	Rorug02G0637900 Rorug04G0140700 Rorug04G0140700
rosa_samantha	Rh3AG039500 Rh3BG040900 Rh3CG039400 Rh3DG040100 Rh4AG202900 Rh4BG200300 Rh4CG214500 Rh4DG200200
rosa_wichuraiana	Rw3G002970 Rw4G017170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1709
Acc65I	GGTACC	1 cut(s)	1306
AccB1I	GGYRCC	1 cut(s)	1306
AccII	CGCG	1 cut(s)	216
AciI	CCGC	5 cut(s)	214, 766, 1108, 1162, 1972
AclWI	GGATC	7 cut(s)	47, 204, 439, 749, 762, 891, 1474
AcsI	RAATTY	5 cut(s)	29, 219, 1341, 1744, 1909
AcuI	CTGAAG	2 cut(s)	42, 1433
AfaI	GTAC	3 cut(s)	13, 331, 1308
AfiI	CCNNNNNNNGG	2 cut(s)	496, 745
AflII	CTTAAG	1 cut(s)	916
AgeI	ACCGGT	1 cut(s)	2191
AjiI	CACGTC	1 cut(s)	1328
AjnI	CCWGG	3 cut(s)	843, 1423, 2071
Alw21I	GWGCWC	2 cut(s)	1457, 1543
Alw26I	GTCTC	2 cut(s)	825, 1928
Alw44I	GTGCAC	1 cut(s)	1539
AlwI	GGATC	7 cut(s)	47, 204, 439, 749, 762, 891, 1474
AlwNI	CAGNNNCTG	2 cut(s)	20, 1475
Ama87I	CYCGRG	2 cut(s)	200, 2014
AoxI	GGCC	5 cut(s)	120, 517, 872, 908, 1159
ApaLI	GTGCAC	1 cut(s)	1539
ApeKI	GCWGC	2 cut(s)	524, 1472
ApoI	RAATTY	5 cut(s)	29, 219, 1341, 1744, 1909
AsiGI	ACCGGT	1 cut(s)	2191
Asp700I	GAANNNNTTC	2 cut(s)	1341, 2024
Asp718I	GGTACC	1 cut(s)	1306
AspLEI	GCGC	1 cut(s)	1520
AspS9I	GGNCC	4 cut(s)	120, 908, 1604, 1864
AsuC2I	CCSGG	1 cut(s)	2177
AsuHPI	GGTGA	6 cut(s)	175, 506, 521, 760, 1301, 1404
AsuII	TTCGAA	2 cut(s)	1491, 1733
AvaI	CYCGRG	2 cut(s)	200, 2014
AvaII	GGWCC	2 cut(s)	1604, 1864
AxyI	CCTNAGG	2 cut(s)	192, 759
BaeGI	GKGCMC	1 cut(s)	1543
BaeI	ACNNNNGTAYC	4 cut(s)	1290, 1298, 1323, 1331
BamHI	GGATCC	1 cut(s)	754
BanI	GGYRCC	1 cut(s)	1306
BanII	GRGCYC	1 cut(s)	1594
BauI	CACGAG	1 cut(s)	2087
BbsI	GAAGAC	1 cut(s)	1122
Bbv12I	GWGCWC	2 cut(s)	1457, 1543
BbvI	GCAGC	2 cut(s)	511, 1459
BccI	CCATC	7 cut(s)	646, 1222, 1280, 1559, 1708, 1815, 2093
BceAI	ACGGC	1 cut(s)	1221
BciT130I	CCWGG	3 cut(s)	845, 1425, 2073
BciVI	GTATCC	1 cut(s)	854
BcnI	CCSGG	1 cut(s)	2177
BcoDI	GTCTC	2 cut(s)	825, 1928
BfaI	CTAG	3 cut(s)	816, 1545, 1881
BfmI	CTRYAG	1 cut(s)	2202
BfrI	CTTAAG	1 cut(s)	916
BfuI	GTATCC	1 cut(s)	854
BglII	AGATCT	1 cut(s)	454
BisI	GCNGC	4 cut(s)	525, 767, 1162, 1473
BlsI	GCNGC	4 cut(s)	526, 768, 1163, 1474
Bme1390I	CCNGG	4 cut(s)	845, 1425, 2073, 2177
Bme18I	GGWCC	2 cut(s)	1604, 1864
BmeT110I	CYCGRG	2 cut(s)	200, 2014
BmgBI	CACGTC	1 cut(s)	1328
BmgT120I	GGNCC	4 cut(s)	120, 908, 1604, 1864
BmiI	GGNNCC	6 cut(s)	488, 582, 756, 1308, 1393, 1865
BmrFI	CCNGG	4 cut(s)	845, 1425, 2073, 2177
BmrI	ACTGGG	1 cut(s)	729
BmsI	GCATC	5 cut(s)	651, 1173, 1418, 2035, 2188
BmuI	ACTGGG	1 cut(s)	729
BpiI	GAAGAC	1 cut(s)	1122
BpmI	CTGGAG	2 cut(s)	567, 827
Bpu14I	TTCGAA	2 cut(s)	1491, 1733
BpuEI	CTTGAG	2 cut(s)	215, 443
BpuMI	CCSGG	1 cut(s)	2177
BsaBI	GATNNNNATC	1 cut(s)	641
BsaI	GGTCTC	1 cut(s)	825
BsaJI	CCNNGG	6 cut(s)	490, 1302, 1480, 1956, 2013, 2071
BsaWI	WCCGGW	5 cut(s)	568, 1309, 1414, 1606, 2191
Bsc4I	CCNNNNNNNGG	2 cut(s)	496, 745
Bse118I	RCCGGY	1 cut(s)	2191
Bse1I	ACTGG	3 cut(s)	64, 724, 2039
Bse21I	CCTNAGG	2 cut(s)	192, 759
Bse3DI	GCAATG	2 cut(s)	1348, 2153
Bse8I	GATNNNNATC	1 cut(s)	641
BseBI	CCWGG	3 cut(s)	845, 1425, 2073
BseDI	CCNNGG	6 cut(s)	490, 1302, 1480, 1956, 2013, 2071
BseGI	GGATG	5 cut(s)	642, 1072, 1164, 1681, 2050
BseJI	GATNNNNATC	1 cut(s)	641
BseLI	CCNNNNNNNGG	2 cut(s)	496, 745
BseMI	GCAATG	2 cut(s)	1348, 2153
BseNI	ACTGG	3 cut(s)	64, 724, 2039
BseSI	GKGCMC	1 cut(s)	1543
BseXI	GCAGC	2 cut(s)	511, 1459
BseYI	CCCAGC	1 cut(s)	1968
BsgI	GTGCAG	2 cut(s)	412, 2156
Bsh1236I	CGCG	1 cut(s)	216
Bsh1285I	CGRYCG	1 cut(s)	2196
BshFI	GGCC	5 cut(s)	122, 519, 874, 910, 1161
BshNI	GGYRCC	1 cut(s)	1306
BshTI	ACCGGT	1 cut(s)	2191
BsiEI	CGRYCG	1 cut(s)	2196
BsiHKAI	GWGCWC	2 cut(s)	1457, 1543
BsiHKCI	CYCGRG	2 cut(s)	200, 2014
BsiSI	CCGG	6 cut(s)	569, 1310, 1415, 1607, 2176, 2192
BslFI	GGGAC	2 cut(s)	1338, 1877
BslI	CCNNNNNNNGG	2 cut(s)	496, 745
BsmAI	GTCTC	2 cut(s)	825, 1928
BsmFI	GGGAC	2 cut(s)	1338, 1877
BsmI	GAATGC	1 cut(s)	686
BsnI	GGCC	5 cut(s)	122, 519, 874, 910, 1161
Bso31I	GGTCTC	1 cut(s)	825
BsoBI	CYCGRG	2 cut(s)	200, 2014
Bsp119I	TTCGAA	2 cut(s)	1491, 1733
Bsp1286I	GDGCHC	3 cut(s)	1457, 1543, 1594
Bsp19I	CCATGG	1 cut(s)	1480
BspACI	CCGC	5 cut(s)	214, 766, 1108, 1162, 1972
BspANI	GGCC	5 cut(s)	122, 519, 874, 910, 1161
BspFNI	CGCG	1 cut(s)	216
BspHI	TCATGA	2 cut(s)	255, 1663
BspLI	GGNNCC	6 cut(s)	488, 582, 756, 1308, 1393, 1865
BspPI	GGATC	7 cut(s)	47, 204, 439, 749, 762, 891, 1474
BspT104I	TTCGAA	2 cut(s)	1491, 1733
BspT107I	GGYRCC	1 cut(s)	1306
BspTI	CTTAAG	1 cut(s)	916
BspTNI	GGTCTC	1 cut(s)	825
BsrDI	GCAATG	2 cut(s)	1348, 2153
BsrFI	RCCGGY	1 cut(s)	2191
BsrI	ACTGG	3 cut(s)	64, 724, 2039
BssAI	RCCGGY	1 cut(s)	2191
BssECI	CCNNGG	6 cut(s)	490, 1302, 1480, 1956, 2013, 2071
BssSI	CACGAG	1 cut(s)	2087
BssT1I	CCWWGG	4 cut(s)	490, 1302, 1480, 1956
Bst2BI	CACGAG	1 cut(s)	2087
Bst2UI	CCWGG	3 cut(s)	845, 1425, 2073
Bst4CI	ACNGT	3 cut(s)	112, 796, 2111
Bst6I	CTCTTC	5 cut(s)	591, 937, 1055, 1488, 1692
BstAFI	CTTAAG	1 cut(s)	916
BstBI	TTCGAA	2 cut(s)	1491, 1733
BstC8I	GCNNGC	3 cut(s)	395, 1571, 2068
BstDSI	CCRYGG	1 cut(s)	1480
BstEII	GGTNACC	1 cut(s)	655
BstENI	CCTNNNNNAGG	1 cut(s)	743
BstF5I	GGATG	5 cut(s)	642, 1072, 1164, 1681, 2050
BstFNI	CGCG	1 cut(s)	216
BstHHI	GCGC	1 cut(s)	1520
BstMAI	GTCTC	2 cut(s)	825, 1928
BstMCI	CGRYCG	1 cut(s)	2196
BstMWI	GCNNNNNNNGC	2 cut(s)	346, 960
BstNI	CCWGG	3 cut(s)	845, 1425, 2073
BstPI	GGTNACC	1 cut(s)	655
BstSCI	CCNGG	4 cut(s)	843, 1423, 2071, 2175
BstSFI	CTRYAG	1 cut(s)	2202
BstSLI	GKGCMC	1 cut(s)	1543
BstUI	CGCG	1 cut(s)	216
BstV1I	GCAGC	2 cut(s)	511, 1459
BstV2I	GAAGAC	1 cut(s)	1122
BstX2I	RGATCY	3 cut(s)	196, 454, 754
BstXI	CCANNNNNNTGG	3 cut(s)	1228, 1565, 1874
BstYI	RGATCY	3 cut(s)	196, 454, 754
Bsu36I	CCTNAGG	2 cut(s)	192, 759
BsuI	GTATCC	1 cut(s)	854
BsuRI	GGCC	5 cut(s)	122, 519, 874, 910, 1161
BtgI	CCRYGG	1 cut(s)	1480
BtgZI	GCGATG	1 cut(s)	1834
BtrI	CACGTC	1 cut(s)	1328
BtsCI	GGATG	5 cut(s)	642, 1072, 1164, 1681, 2050
BtsI	GCAGTG	1 cut(s)	384
BtsIMutI	CAGTG	4 cut(s)	384, 717, 1457, 2046
Cac8I	GCNNGC	3 cut(s)	395, 1571, 2068
CaiI	CAGNNNCTG	2 cut(s)	20, 1475
CciI	TCATGA	2 cut(s)	255, 1663
CfoI	GCGC	1 cut(s)	1520
Cfr10I	RCCGGY	1 cut(s)	2191
Cfr13I	GGNCC	4 cut(s)	120, 908, 1604, 1864
Csp6I	GTAC	3 cut(s)	12, 330, 1307
CspAI	ACCGGT	1 cut(s)	2191
CviAII	CATG	7 cut(s)	153, 256, 431, 577, 971, 1481, 1664
CviQI	GTAC	3 cut(s)	12, 330, 1307
Eam1104I	CTCTTC	5 cut(s)	591, 937, 1055, 1488, 1692
EarI	CTCTTC	5 cut(s)	591, 937, 1055, 1488, 1692
EciI	GGCGGA	1 cut(s)	1123
Eco130I	CCWWGG	4 cut(s)	490, 1302, 1480, 1956
Eco24I	GRGCYC	1 cut(s)	1594
Eco31I	GGTCTC	1 cut(s)	825
Eco32I	GATATC	3 cut(s)	1246, 1585, 1645
Eco47I	GGWCC	2 cut(s)	1604, 1864
Eco57I	CTGAAG	2 cut(s)	42, 1433
Eco81I	CCTNAGG	2 cut(s)	192, 759
Eco88I	CYCGRG	2 cut(s)	200, 2014
Eco91I	GGTNACC	1 cut(s)	655
EcoNI	CCTNNNNNAGG	1 cut(s)	743
EcoO109I	RGGNCCY	2 cut(s)	120, 908
EcoO65I	GGTNACC	1 cut(s)	655
EcoRI	GAATTC	1 cut(s)	29
EcoRII	CCWGG	3 cut(s)	843, 1423, 2071
EcoRV	GATATC	3 cut(s)	1246, 1585, 1645
EcoT14I	CCWWGG	4 cut(s)	490, 1302, 1480, 1956
EcoT22I	ATGCAT	1 cut(s)	1411
EcoT38I	GRGCYC	1 cut(s)	1594
ErhI	CCWWGG	4 cut(s)	490, 1302, 1480, 1956
FaeI	CATG	7 cut(s)	156, 259, 434, 580, 974, 1484, 1667
FaqI	GGGAC	2 cut(s)	1338, 1877
FatI	CATG	7 cut(s)	152, 255, 430, 576, 970, 1480, 1663
Fnu4HI	GCNGC	4 cut(s)	525, 767, 1162, 1473
FokI	GGATG	5 cut(s)	649, 1079, 1151, 1688, 2057
FriOI	GRGCYC	1 cut(s)	1594
Fsp4HI	GCNGC	4 cut(s)	525, 767, 1162, 1473
FspBI	CTAG	3 cut(s)	816, 1545, 1881
GlaI	GCGC	1 cut(s)	1519
GluI	GCNGC	4 cut(s)	525, 767, 1162, 1473
GsaI	CCCAGC	1 cut(s)	1972
GsuI	CTGGAG	2 cut(s)	567, 827
HaeIII	GGCC	5 cut(s)	122, 519, 874, 910, 1161
HapII	CCGG	6 cut(s)	569, 1310, 1415, 1607, 2176, 2192
HhaI	GCGC	1 cut(s)	1520
Hin1II	CATG	7 cut(s)	156, 259, 434, 580, 974, 1484, 1667
Hin6I	GCGC	1 cut(s)	1518
HinP1I	GCGC	1 cut(s)	1518
HincII	GTYRAC	2 cut(s)	619, 1087
HindII	GTYRAC	2 cut(s)	619, 1087
HindIII	AAGCTT	2 cut(s)	167, 1890
HinfI	GANTC	3 cut(s)	157, 1634, 1730
HpaII	CCGG	6 cut(s)	569, 1310, 1415, 1607, 2176, 2192
HphI	GGTGA	6 cut(s)	175, 506, 521, 760, 1301, 1404
Hpy166II	GTNNAC	5 cut(s)	321, 592, 619, 1087, 1541
Hpy8I	GTNNAC	5 cut(s)	321, 592, 619, 1087, 1541
HpyAV	CCTTC	6 cut(s)	131, 745, 1151, 1508, 1595, 2004
HpyCH4III	ACNGT	3 cut(s)	112, 796, 2111
HpyCH4IV	ACGT	3 cut(s)	1327, 1486, 1835
HpyF10VI	GCNNNNNNNGC	2 cut(s)	346, 960
HpySE526I	ACGT	3 cut(s)	1327, 1486, 1835
Hsp92II	CATG	7 cut(s)	156, 259, 434, 580, 974, 1484, 1667
HspAI	GCGC	1 cut(s)	1518
KpnI	GGTACC	1 cut(s)	1310
LmnI	GCTCC	5 cut(s)	98, 586, 820, 1877, 1943
Lsp1109I	GCAGC	2 cut(s)	511, 1459
LweI	GCATC	5 cut(s)	651, 1173, 1418, 2035, 2188
MaeI	CTAG	3 cut(s)	816, 1545, 1881
MaeII	ACGT	3 cut(s)	1327, 1486, 1835
MaeIII	GTNAC	8 cut(s)	307, 464, 655, 790, 947, 986, 1261, 1678
MflI	RGATCY	3 cut(s)	196, 454, 754
MhlI	GDGCHC	3 cut(s)	1457, 1543, 1594
MmeI	TCCRAC	1 cut(s)	541
Mph1103I	ATGCAT	1 cut(s)	1411
MroXI	GAANNNNTTC	2 cut(s)	1341, 2024
MseI	TTAA	6 cut(s)	366, 869, 917, 1133, 1320, 1613
MslI	CAYNNNNRTG	2 cut(s)	429, 1563
MspA1I	CMGCKG	2 cut(s)	1472, 1972
MspCI	CTTAAG	1 cut(s)	916
MspI	CCGG	6 cut(s)	569, 1310, 1415, 1607, 2176, 2192
MspR9I	CCNGG	4 cut(s)	845, 1425, 2073, 2177
Mva1269I	GAATGC	1 cut(s)	686
MvaI	CCWGG	3 cut(s)	845, 1425, 2073
MvnI	CGCG	1 cut(s)	216
MwoI	GCNNNNNNNGC	2 cut(s)	346, 960
NciI	CCSGG	1 cut(s)	2177
NcoI	CCATGG	1 cut(s)	1480
NlaIII	CATG	7 cut(s)	156, 259, 434, 580, 974, 1484, 1667
NlaIV	GGNNCC	6 cut(s)	488, 582, 756, 1308, 1393, 1865
NmuCI	GTSAC	2 cut(s)	464, 790
NsiI	ATGCAT	1 cut(s)	1411
NspV	TTCGAA	2 cut(s)	1491, 1733
PaeR7I	CTCGAG	1 cut(s)	200
PagI	TCATGA	2 cut(s)	255, 1663
PctI	GAATGC	1 cut(s)	686
PdmI	GAANNNNTTC	2 cut(s)	1341, 2024
PfeI	GAWTC	3 cut(s)	157, 1634, 1730
PflFI	GACNNNGTC	1 cut(s)	796
PinAI	ACCGGT	1 cut(s)	2191
PkrI	GCNGC	4 cut(s)	526, 768, 1163, 1474
PsiI	TTATAA	1 cut(s)	1709
Psp6I	CCWGG	3 cut(s)	843, 1423, 2071
PspEI	GGTNACC	1 cut(s)	655
PspFI	CCCAGC	1 cut(s)	1968
PspGI	CCWGG	3 cut(s)	843, 1423, 2071
PspN4I	GGNNCC	6 cut(s)	488, 582, 756, 1308, 1393, 1865
PspPI	GGNCC	4 cut(s)	120, 908, 1604, 1864
PstNI	CAGNNNCTG	2 cut(s)	20, 1475
PsuI	RGATCY	3 cut(s)	196, 454, 754
PsyI	GACNNNGTC	1 cut(s)	796
PvuII	CAGCTG	1 cut(s)	1472
RsaI	GTAC	3 cut(s)	13, 331, 1308
RsaNI	GTAC	3 cut(s)	12, 330, 1307
RseI	CAYNNNNRTG	2 cut(s)	429, 1563
SaqAI	TTAA	6 cut(s)	366, 869, 917, 1133, 1320, 1613
SatI	GCNGC	4 cut(s)	525, 767, 1162, 1473
Sau96I	GGNCC	4 cut(s)	120, 908, 1604, 1864
ScrFI	CCNGG	4 cut(s)	845, 1425, 2073, 2177
SduI	GDGCHC	3 cut(s)	1457, 1543, 1594
SfaNI	GCATC	5 cut(s)	651, 1173, 1418, 2035, 2188
SfcI	CTRYAG	1 cut(s)	2202
Sfr274I	CTCGAG	1 cut(s)	200
SfuI	TTCGAA	2 cut(s)	1491, 1733
SinI	GGWCC	2 cut(s)	1604, 1864
SlaI	CTCGAG	1 cut(s)	200
SmiMI	CAYNNNNRTG	2 cut(s)	429, 1563
SmlI	CTYRAG	4 cut(s)	200, 230, 458, 916
SmoI	CTYRAG	4 cut(s)	200, 230, 458, 916
SsiI	CCGC	5 cut(s)	214, 766, 1108, 1162, 1972
SspMI	CTAG	3 cut(s)	816, 1545, 1881
StyD4I	CCNGG	4 cut(s)	843, 1423, 2071, 2175
StyI	CCWWGG	4 cut(s)	490, 1302, 1480, 1956
TaaI	ACNGT	3 cut(s)	112, 796, 2111
TaiI	ACGT	3 cut(s)	1330, 1489, 1838
TaqI	TCGA	5 cut(s)	201, 1491, 1637, 1733, 2196
TaqII	GACCGA	1 cut(s)	1900
TauI	GCSGC	2 cut(s)	769, 1164
TfiI	GAWTC	3 cut(s)	157, 1634, 1730
Tru1I	TTAA	6 cut(s)	366, 869, 917, 1133, 1320, 1613
Tru9I	TTAA	6 cut(s)	366, 869, 917, 1133, 1320, 1613
TscAI	CASTG	4 cut(s)	384, 724, 1457, 2046
TseFI	GTSAC	2 cut(s)	464, 790
TseI	GCWGC	2 cut(s)	524, 1472
Tsp45I	GTSAC	2 cut(s)	464, 790
TspDTI	ATGAA	9 cut(s)	17, 150, 212, 272, 466, 565, 915, 929, 1680
TspGWI	ACGGA	3 cut(s)	524, 873, 1479
TspRI	CASTG	4 cut(s)	384, 724, 1457, 2046
Tth111I	GACNNNGTC	1 cut(s)	796
Vha464I	CTTAAG	1 cut(s)	916
VneI	GTGCAC	1 cut(s)	1539
VpaK11BI	GGWCC	2 cut(s)	1604, 1864
XagI	CCTNNNNNAGG	1 cut(s)	743
XapI	RAATTY	5 cut(s)	29, 219, 1341, 1744, 1909
XhoI	CTCGAG	1 cut(s)	200
XmnI	GAANNNNTTC	2 cut(s)	1341, 2024
XspI	CTAG	3 cut(s)	816, 1545, 1881
Zsp2I	ATGCAT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.