Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0005285

Physical Location & Seq

Genomic Coordinates Forward (+)

1344551 .. 1345763

Gene Structure 1213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1004600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGATGAGCAATACATGGAGGGACGAACAACATCCATCCTTCATCAACTTCATCTCCACTTTCCTCGCCGCAAACTCTTTCCGCCTCAACTTTGTCCCAATTACCCCCGACTTCATTTTCAACTGTGGGGGTTCATCTGTGGCATTCATTTTCATGACTAGCTTGGATTCCGGTACCATCTCGCAATTCTTCGGCAGAGTTCAGAAGCTGAAGCTGCAATTTACAAATCTATATGTTGTCATCACGCTCCCAACCAAGGAGCAAAACGATTTGTTTGTTCGTTCTTACTTCAAATTTGGGATGGAACTCGGCAAGCCAACATTCGTTCTGGTTAAGGACTTGGAAATGGGGTTCGAGAAGATAACGAAAATAGCTCATTCTCGTGGGGTGTGCAAGCGAGGGGATGCCACAACAAAATTGAAGGCTGAGGACCTCAAAACATCCCCATTTTGTTCAATTTTGTTGGAGAAGAGGAAGCAAACAGTGCAAACACCAGGCATATTTCAAAAAGTGATCACAGCCATTCCGGGCATCGACAATCATGATGCAAATGCGCTTAACCAGGCTATCGGATCGATCGAAGGAATTGCCAAAGCATCAAAGGAGCACATTATGGAAAACACAGACCTCTCAGCTGACAAGGCACAAGTGCTTTCAAGGTTTTTCAGAGATCCAAAGTTTTACCTCAGTCCTAAGATCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000018 GO:0000280 GO:0000712 GO:0003006 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0007140 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009555 GO:0009893 GO:0009987 GO:0010520 GO:0010564 GO:0010604 GO:0010638 GO:0010845 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0031323 GO:0031325 GO:0032501 GO:0032502 GO:0032504 GO:0033043 GO:0034293 GO:0034641 GO:0035825 GO:0040020 GO:0043170 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044703 GO:0045132 GO:0045787 GO:0045836 GO:0045911 GO:0045935 GO:0046483 GO:0048229 GO:0048232 GO:0048236 GO:0048285 GO:0048518 GO:0048522 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051304 GO:0051307 GO:0051321 GO:0051445 GO:0051446 GO:0051704 GO:0051726 GO:0051783 GO:0051785 GO:0060255 GO:0060631 GO:0060903 GO:0061982 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090068 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HHH_2 HHH_5

Protein Analysis

235

Amino Acids

26.35

Weight (kDa)

9.32

Isoelectric Point (pI)

25.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HHH_5	PF14520	172 - 218	3.1e-06	Helix-hairpin-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005285)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12790
fragaria_vesca	FvH4_2g00851 FvH4_2g00851
malus_domestica	MD05G1003900.v1.1 MD05G1004600.v1.1
prunus_persica	Prupe.8G007100_v2.0.a1 Prupe.8G007100_v2.0.a1 Prupe.8G007100_v2.0.a1 Prupe.8G007100_v2.0.a1 Prupe.8G007100_v2.0.a1 Prupe.8G007100_v2.0.a1
pyrus_communis	pycom05g00340
rosa_chinensis	RchiOBHm_Chr6g0251241 RchiOBHm_Chr6g0275321
rosa_laevigata	RLG00000015471
rosa_multiflora	Rmu_sc0001637.1_g000004 Rmu_sc0015396.1_g000004
rosa_roxburghii	Rroxscaffold_7G00216890
rosa_rugosa	Rorug05G0501100
rosa_samantha	Rh6AG010500 Rh6BG001500 Rh6BG001600 Rh6CG007500 Rh6DG007900
rosa_wichuraiana	Rw2G011930 Rw5G012820 Rw6G001020 Rw6G001130 Rw6G001190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	173
AccB1I	GGYRCC	1 cut(s)	173
AciI	CCGC	2 cut(s)	69, 82
AclWI	GGATC	2 cut(s)	580, 665
AcsI	RAATTY	1 cut(s)	293
AcuI	CTGAAG	1 cut(s)	230
AfaI	GTAC	1 cut(s)	175
AfiI	CCNNNNNNNGG	1 cut(s)	256
AgsI	TTSAA	6 cut(s)	121, 292, 421, 456, 506, 657
AjnI	CCWGG	2 cut(s)	493, 561
AloI	GAACNNNNNNTCC	2 cut(s)	335, 367
AluBI	AGCT	5 cut(s)	162, 208, 214, 374, 635
AluI	AGCT	5 cut(s)	162, 208, 214, 374, 635
Alw21I	GWGCWC	1 cut(s)	609
AlwI	GGATC	2 cut(s)	580, 665
AlwNI	CAGNNNCTG	1 cut(s)	208
ApeKI	GCWGC	1 cut(s)	214
ApoI	RAATTY	1 cut(s)	293
Asp718I	GGTACC	1 cut(s)	173
AspLEI	GCGC	1 cut(s)	556
AspS9I	GGNCC	1 cut(s)	430
AsuC2I	CCSGG	1 cut(s)	528
AvaII	GGWCC	1 cut(s)	430
BanI	GGYRCC	1 cut(s)	173
BauI	CACGAG	1 cut(s)	381
Bbv12I	GWGCWC	1 cut(s)	609
BbvCI	CCTCAGC	1 cut(s)	426
BbvI	GCAGC	1 cut(s)	201
BccI	CCATC	3 cut(s)	43, 185, 295
BcgI	CGANNNNNNTGC	2 cut(s)	569, 603
BciT130I	CCWGG	2 cut(s)	495, 563
BclI	TGATCA	1 cut(s)	513
BcnI	CCSGG	1 cut(s)	528
BfaI	CTAG	1 cut(s)	159
BisI	GCNGC	2 cut(s)	69, 215
BlsI	GCNGC	2 cut(s)	70, 216
Bme1390I	CCNGG	3 cut(s)	495, 528, 563
Bme18I	GGWCC	1 cut(s)	430
BmgT120I	GGNCC	1 cut(s)	430
BmiI	GGNNCC	1 cut(s)	175
BmrFI	CCNGG	3 cut(s)	495, 528, 563
BmsI	GCATC	4 cut(s)	394, 535, 540, 605
Bpu10I	CCTNAGC	1 cut(s)	426
BpuMI	CCSGG	1 cut(s)	528
Bsa29I	ATCGAT	1 cut(s)	575
BsaJI	CCNNGG	1 cut(s)	255
BsaWI	WCCGGW	1 cut(s)	170
BsaXI	ACNNNNNCTCC	2 cut(s)	38, 68
Bsc4I	CCNNNNNNNGG	1 cut(s)	256
BseBI	CCWGG	2 cut(s)	495, 563
BseCI	ATCGAT	1 cut(s)	575
BseDI	CCNNGG	1 cut(s)	255
BseGI	GGATG	5 cut(s)	31, 35, 306, 409, 440
BseLI	CCNNNNNNNGG	1 cut(s)	256
BseMII	CTCAG	3 cut(s)	417, 645, 700
BseXI	GCAGC	1 cut(s)	201
Bsh1285I	CGRYCG	1 cut(s)	579
BshNI	GGYRCC	1 cut(s)	173
BshVI	ATCGAT	1 cut(s)	575
BsiEI	CGRYCG	1 cut(s)	579
BsiHKAI	GWGCWC	1 cut(s)	609
BsiSI	CCGG	2 cut(s)	171, 527
BslFI	GGGAC	2 cut(s)	35, 80
BslI	CCNNNNNNNGG	1 cut(s)	256
BsmFI	GGGAC	2 cut(s)	35, 80
BsmI	GAATGC	1 cut(s)	143
Bsp1286I	GDGCHC	1 cut(s)	609
Bsp143I	GATC	5 cut(s)	513, 572, 576, 670, 696
BspACI	CCGC	2 cut(s)	69, 82
BspCNI	CTCAG	3 cut(s)	418, 644, 699
BspDI	ATCGAT	1 cut(s)	575
BspHI	TCATGA	2 cut(s)	153, 541
BspLI	GGNNCC	1 cut(s)	175
BspPI	GGATC	2 cut(s)	580, 665
BspT107I	GGYRCC	1 cut(s)	173
BssECI	CCNNGG	1 cut(s)	255
BssMI	GATC	5 cut(s)	513, 572, 576, 670, 696
BssSI	CACGAG	1 cut(s)	381
BssT1I	CCWWGG	1 cut(s)	255
Bst2BI	CACGAG	1 cut(s)	381
Bst2UI	CCWGG	2 cut(s)	495, 563
Bst4CI	ACNGT	2 cut(s)	125, 484
Bst6I	CTCTTC	1 cut(s)	464
BstAPI	GCANNNNNTGC	1 cut(s)	484
BstC8I	GCNNGC	2 cut(s)	314, 395
BstDEI	CTNAG	4 cut(s)	426, 631, 686, 693
BstF5I	GGATG	5 cut(s)	31, 35, 306, 409, 440
BstHHI	GCGC	1 cut(s)	556
BstKTI	GATC	5 cut(s)	516, 575, 579, 673, 699
BstMBI	GATC	5 cut(s)	513, 572, 576, 670, 696
BstMCI	CGRYCG	1 cut(s)	579
BstMWI	GCNNNNNNNGC	3 cut(s)	214, 484, 641
BstNI	CCWGG	2 cut(s)	495, 563
BstSCI	CCNGG	3 cut(s)	493, 526, 561
BstV1I	GCAGC	1 cut(s)	201
BstX2I	RGATCY	1 cut(s)	670
BstYI	RGATCY	1 cut(s)	670
Bsu15I	ATCGAT	1 cut(s)	575
BsuTUI	ATCGAT	1 cut(s)	575
BtsCI	GGATG	5 cut(s)	31, 35, 306, 409, 440
BtsIMutI	CAGTG	1 cut(s)	489
Cac8I	GCNNGC	2 cut(s)	314, 395
CaiI	CAGNNNCTG	1 cut(s)	208
CciI	TCATGA	2 cut(s)	153, 541
CfoI	GCGC	1 cut(s)	556
Cfr13I	GGNCC	1 cut(s)	430
ClaI	ATCGAT	1 cut(s)	575
Csp6I	GTAC	1 cut(s)	174
CviAII	CATG	3 cut(s)	15, 154, 542
CviJI	RGCY	9 cut(s)	162, 208, 214, 316, 374, 425, 521, 566, 635
CviKI_1	RGCY	9 cut(s)	162, 208, 214, 316, 374, 425, 521, 566, 635
CviQI	GTAC	1 cut(s)	174
DdeI	CTNAG	4 cut(s)	426, 631, 686, 693
DpnI	GATC	5 cut(s)	515, 574, 578, 672, 698
DpnII	GATC	5 cut(s)	513, 572, 576, 670, 696
Eam1104I	CTCTTC	1 cut(s)	464
EarI	CTCTTC	1 cut(s)	464
EciI	GGCGGA	1 cut(s)	71
Eco130I	CCWWGG	1 cut(s)	255
Eco47I	GGWCC	1 cut(s)	430
Eco57I	CTGAAG	1 cut(s)	230
EcoO109I	RGGNCCY	1 cut(s)	430
EcoRII	CCWGG	2 cut(s)	493, 561
EcoT14I	CCWWGG	1 cut(s)	255
ErhI	CCWWGG	1 cut(s)	255
FaeI	CATG	3 cut(s)	18, 157, 545
FaiI	YATR	7 cut(s)	16, 155, 232, 234, 500, 543, 614
FaqI	GGGAC	2 cut(s)	35, 80
FatI	CATG	3 cut(s)	14, 153, 541
FbaI	TGATCA	1 cut(s)	513
Fnu4HI	GCNGC	2 cut(s)	69, 215
FokI	GGATG	5 cut(s)	18, 22, 313, 416, 427
Fsp4HI	GCNGC	2 cut(s)	69, 215
FspBI	CTAG	1 cut(s)	159
GlaI	GCGC	1 cut(s)	555
GluI	GCNGC	2 cut(s)	69, 215
HapII	CCGG	2 cut(s)	171, 527
HhaI	GCGC	1 cut(s)	556
Hin1II	CATG	3 cut(s)	18, 157, 545
Hin6I	GCGC	1 cut(s)	554
HinP1I	GCGC	1 cut(s)	554
HinfI	GANTC	1 cut(s)	167
HpaII	CCGG	2 cut(s)	171, 527
Hpy188I	TCNGA	3 cut(s)	204, 572, 668
Hpy188III	TCNNGA	3 cut(s)	154, 355, 542
HpyAV	CCTTC	3 cut(s)	49, 415, 575
HpyCH4III	ACNGT	2 cut(s)	125, 484
HpyCH4V	TGCA	4 cut(s)	217, 393, 487, 548
HpyF10VI	GCNNNNNNNGC	3 cut(s)	214, 484, 641
HpyF3I	CTNAG	4 cut(s)	426, 631, 686, 693
Hsp92II	CATG	3 cut(s)	18, 157, 545
HspAI	GCGC	1 cut(s)	554
KpnI	GGTACC	1 cut(s)	177
Ksp22I	TGATCA	1 cut(s)	513
Kzo9I	GATC	5 cut(s)	513, 572, 576, 670, 696
LmnI	GCTCC	3 cut(s)	252, 259, 604
LpnPI	CCDG	7 cut(s)	184, 314, 480, 507, 540, 548, 575
Lsp1109I	GCAGC	1 cut(s)	201
LweI	GCATC	4 cut(s)	394, 535, 540, 605
MaeI	CTAG	1 cut(s)	159
MalI	GATC	5 cut(s)	515, 574, 578, 672, 698
MboI	GATC	5 cut(s)	513, 572, 576, 670, 696
MboII	GAAGA	3 cut(s)	181, 370, 481
MflI	RGATCY	1 cut(s)	670
MhlI	GDGCHC	1 cut(s)	609
MluCI	AATT	7 cut(s)	99, 185, 218, 293, 416, 456, 585
MmeI	TCCRAC	1 cut(s)	444
MnlI	CCTC	9 cut(s)	12, 74, 95, 392, 421, 443, 465, 638, 695
MseI	TTAA	2 cut(s)	333, 558
MslI	CAYNNNNRTG	2 cut(s)	152, 381
MspA1I	CMGCKG	1 cut(s)	635
MspI	CCGG	2 cut(s)	171, 527
MspR9I	CCNGG	3 cut(s)	495, 528, 563
Mva1269I	GAATGC	1 cut(s)	143
MvaI	CCWGG	2 cut(s)	495, 563
MwoI	GCNNNNNNNGC	3 cut(s)	214, 484, 641
NciI	CCSGG	1 cut(s)	528
NdeII	GATC	5 cut(s)	513, 572, 576, 670, 696
NlaIII	CATG	3 cut(s)	18, 157, 545
NlaIV	GGNNCC	1 cut(s)	175
NmeAIII	GCCGAG	1 cut(s)	288
PagI	TCATGA	2 cut(s)	153, 541
PcsI	WCGNNNNNNNCGW	1 cut(s)	576
PctI	GAATGC	1 cut(s)	143
PfeI	GAWTC	1 cut(s)	167
PkrI	GCNGC	2 cut(s)	70, 216
Ple19I	CGATCG	1 cut(s)	579
PpuMI	RGGWCCY	1 cut(s)	430
Psp5II	RGGWCCY	1 cut(s)	430
Psp6I	CCWGG	2 cut(s)	493, 561
PspGI	CCWGG	2 cut(s)	493, 561
PspN4I	GGNNCC	1 cut(s)	175
PspPI	GGNCC	1 cut(s)	430
PspPPI	RGGWCCY	1 cut(s)	430
PstNI	CAGNNNCTG	1 cut(s)	208
PsuI	RGATCY	1 cut(s)	670
PvuI	CGATCG	1 cut(s)	579
PvuII	CAGCTG	1 cut(s)	635
RsaI	GTAC	1 cut(s)	175
RsaNI	GTAC	1 cut(s)	174
RseI	CAYNNNNRTG	2 cut(s)	152, 381
SaqAI	TTAA	2 cut(s)	333, 558
SatI	GCNGC	2 cut(s)	69, 215
Sau3AI	GATC	5 cut(s)	513, 572, 576, 670, 696
Sau96I	GGNCC	1 cut(s)	430
ScrFI	CCNGG	3 cut(s)	495, 528, 563
SduI	GDGCHC	1 cut(s)	609
SetI	ASST	9 cut(s)	164, 210, 216, 376, 435, 630, 637, 662, 687
SfaNI	GCATC	4 cut(s)	394, 535, 540, 605
SinI	GGWCC	1 cut(s)	430
SmiMI	CAYNNNNRTG	2 cut(s)	152, 381
Sse9I	AATT	7 cut(s)	99, 185, 218, 293, 416, 456, 585
SsiI	CCGC	2 cut(s)	69, 82
SspMI	CTAG	1 cut(s)	159
StyD4I	CCNGG	3 cut(s)	493, 526, 561
StyI	CCWWGG	1 cut(s)	255
TaaI	ACNGT	2 cut(s)	125, 484
TaqI	TCGA	4 cut(s)	354, 534, 575, 579
TasI	AATT	7 cut(s)	99, 185, 218, 293, 416, 456, 585
TauI	GCSGC	1 cut(s)	71
TfiI	GAWTC	1 cut(s)	167
Tru1I	TTAA	2 cut(s)	333, 558
Tru9I	TTAA	2 cut(s)	333, 558
TscAI	CASTG	1 cut(s)	489
TseI	GCWGC	1 cut(s)	214
TspDTI	ATGAA	6 cut(s)	31, 40, 103, 123, 136, 142
TspRI	CASTG	1 cut(s)	489
VpaK11BI	GGWCC	1 cut(s)	430
XapI	RAATTY	1 cut(s)	293
XspI	CTAG	1 cut(s)	159

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.