MD05G1042300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014098
Physical Location & Seq
Reverse (-)
7125723 .. 7126412
690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1042300.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAGTCCTCCTCACCTTCTGATCATGTAAATGGTGGACGATGCCAAACCCAAACTGTCTCAAAAAATTGTGACACAATATTTCCAGCCATGAAGAAGTATCAATATTCTTCTCAAAGAACACCATCATTTTCTTCCTCCTCATCGTGGTCGTCGTCTTCATCTTTCGTATCATCGTCCTGTAATCTCGACCATGATTCTTCTCCTCCTATTAGTCCGGCAACCCCGCTCCAGTTTTCCGGTGTCCCATTCTCTTGGGAACATTTTCCCGGTATTCCAAAGAAACCTAGCAGCAACAACAAGGAATTACAATTACATTCCTCATTGAAGCGTCTCCCACAGCCTCCTCCCACACTCAGTACTCAGAAGATCTCCTCCAAGAAGTTGATCATGGATGATATTATTCAGATCAGACACAACTCCAAAAGGCATACTGCTAATCTCCCAAGGGATCCTTTCTTTGCTGCACTAGTTGAGTGTTCCAAGGACGACGACGGCAACAACAACGACGATGATCAAGGATACTCAAGTGGTGCTGCCAAGGTCTCAAGGAGTGTGAGTGAAAGGTTTGGATTTTTAAATCTTTCTGCATTATCTTGTAAGACAACTTGTGCAGTTTCTGAATCACTCATACACGTTCAAAGGCCGAGTACAACTTCTTATCATCTGATTAATCGCCGTTCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

230

Amino Acids

25.21

Weight (kDa)

9.18

Isoelectric Point (pI)

68.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF688 PF05097 80 - 187 2.9e-06 Protein of unknown function (DUF688)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22680 AT1G71970
fragaria_vesca FvH4_2g11630
malus_domestica MD05G1042300.v1.1
prunus_persica Prupe.8G058900_v2.0.a1
pyrus_communis pycom05g03490 pycom10g03480
rosa_chinensis RchiOBHm_Chr6g0270871
rosa_laevigata RLG00000013763
rosa_multiflora Rmu_sc0010417.1_g000001
rosa_roxburghii Rroxscaffold_3G00238570 Rroxscaffold_7G00197560
rosa_samantha Rh6AG176400 Rh6BG179800 Rh6CG175800 Rh6DG168100
rosa_wichuraiana Rw6G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 229
AciI CCGC 1 cut(s) 227
AclWI GGATC 2 cut(s) 446, 459
AfaI GTAC 2 cut(s) 361, 652
AfiI CCNNNNNNNGG 1 cut(s) 147
AflIII ACRYGT 1 cut(s) 634
AgsI TTSAA 2 cut(s) 328, 641
AhlI ACTAGT 1 cut(s) 469
Alw26I GTCTC 3 cut(s) 64, 338, 550
AlwI GGATC 2 cut(s) 446, 459
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 3 cut(s) 291, 464, 536
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 264
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 6
BaeI ACNNNNGTAYC 2 cut(s) 514, 547
BamHI GGATCC 1 cut(s) 451
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 303, 451, 523
BccI CCATC 1 cut(s) 133
BceAI ACGGC 2 cut(s) 511, 663
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 3 cut(s) 22, 387, 514
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 64, 338, 550
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 2 cut(s) 288, 470
BfuI GTATCC 1 cut(s) 515
BglII AGATCT 1 cut(s) 369
BisI GCNGC 3 cut(s) 292, 465, 537
BlsI GCNGC 3 cut(s) 293, 466, 538
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 1 cut(s) 270
BmiI GGNNCC 1 cut(s) 453
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 32
BpiI GAAGAC 1 cut(s) 150
BplI GAGNNNNNCTC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 215
BpuEI CTTGAG 2 cut(s) 511, 532
BpuMI CCSGG 1 cut(s) 270
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 3 cut(s) 446, 483, 540
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 446, 483, 540
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMII CTCAG 2 cut(s) 370, 377
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 4 cut(s) 130, 195, 336, 364
BseXI GCAGC 3 cut(s) 303, 451, 523
BsgI GTGCAG 2 cut(s) 450, 633
BshFI GGCC 1 cut(s) 646
BsiSI CCGG 3 cut(s) 218, 240, 270
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 3 cut(s) 64, 338, 550
BsmBI CGTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 1 cut(s) 646
Bso31I GGTCTC 1 cut(s) 550
Bsp143I GATC 6 cut(s) 22, 369, 387, 408, 451, 514
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 2 cut(s) 369, 376
BspLI GGNNCC 1 cut(s) 453
BspPI GGATC 2 cut(s) 446, 459
BspTNI GGTCTC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 229
BsrI ACTGG 1 cut(s) 232
BssECI CCNNGG 3 cut(s) 446, 483, 540
BssMI GATC 6 cut(s) 22, 369, 387, 408, 451, 514
BssT1I CCWWGG 3 cut(s) 446, 483, 540
Bst4CI ACNGT 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 356, 363
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 6 cut(s) 25, 372, 390, 411, 454, 517
BstMAI GTCTC 3 cut(s) 64, 338, 550
BstMBI GATC 6 cut(s) 22, 369, 387, 408, 451, 514
BstSCI CCNGG 1 cut(s) 268
BstV1I GCAGC 3 cut(s) 303, 451, 523
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 369, 451
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 2 cut(s) 369, 451
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 1 cut(s) 646
BtsCI GGATG 1 cut(s) 400
CaiI CAGNNNCTG 1 cut(s) 620
CseI GACGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 360, 651
CviAII CATG 4 cut(s) 26, 91, 194, 391
CviJI RGCY 3 cut(s) 89, 343, 646
CviKI_1 RGCY 3 cut(s) 89, 343, 646
CviQI GTAC 2 cut(s) 360, 651
DdeI CTNAG 2 cut(s) 356, 363
DpnI GATC 6 cut(s) 24, 371, 389, 410, 453, 516
DpnII GATC 6 cut(s) 22, 369, 387, 408, 451, 514
DraI TTTAAA 1 cut(s) 579
Eco130I CCWWGG 3 cut(s) 446, 483, 540
Eco31I GGTCTC 1 cut(s) 550
EcoT14I CCWWGG 3 cut(s) 446, 483, 540
ErhI CCWWGG 3 cut(s) 446, 483, 540
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 4 cut(s) 29, 94, 197, 394
FaiI YATR 6 cut(s) 27, 92, 195, 392, 432, 632
FalI AAGNNNNNCTT 2 cut(s) 439, 471
FaqI GGGAC 1 cut(s) 230
FatI CATG 4 cut(s) 25, 90, 193, 390
FauI CCCGC 1 cut(s) 234
FbaI TGATCA 3 cut(s) 22, 387, 514
Fnu4HI GCNGC 3 cut(s) 292, 465, 537
FokI GGATG 1 cut(s) 407
Fsp4HI GCNGC 3 cut(s) 292, 465, 537
FspBI CTAG 2 cut(s) 288, 470
GluI GCNGC 3 cut(s) 292, 465, 537
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 646
HapII CCGG 3 cut(s) 218, 240, 270
HgaI GACGC 1 cut(s) 320
Hin1II CATG 4 cut(s) 29, 94, 197, 394
HinfI GANTC 3 cut(s) 5, 197, 623
HpaII CCGG 3 cut(s) 218, 240, 270
HphI GGTGA 1 cut(s) 6
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 6 cut(s) 22, 366, 408, 413, 622, 669
Hpy188III TCNNGA 2 cut(s) 188, 684
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 4 cut(s) 157, 494, 497, 512
HpyAV CCTTC 1 cut(s) 27
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 1 cut(s) 636
HpyCH4V TGCA 3 cut(s) 467, 590, 614
HpyF3I CTNAG 2 cut(s) 356, 363
HpySE526I ACGT 1 cut(s) 636
Hsp92II CATG 4 cut(s) 29, 94, 197, 394
Ksp22I TGATCA 3 cut(s) 22, 387, 514
Kzo9I GATC 6 cut(s) 22, 369, 387, 408, 451, 514
LmnI GCTCC 1 cut(s) 234
LpnPI CCDG 6 cut(s) 99, 193, 231, 245, 253, 283
Lsp1109I GCAGC 3 cut(s) 303, 451, 523
LweI GCATC 1 cut(s) 32
MaeI CTAG 2 cut(s) 288, 470
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 1 cut(s) 71
MalI GATC 6 cut(s) 24, 371, 389, 410, 453, 516
MbiI CCGCTC 1 cut(s) 229
MboI GATC 6 cut(s) 22, 369, 387, 408, 451, 514
MboII GAAGA 6 cut(s) 102, 106, 126, 150, 192, 379
MflI RGATCY 2 cut(s) 369, 451
MluCI AATT 3 cut(s) 67, 305, 311
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 19, 22, 148, 151, 216, 331, 354, 357, 385
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 2 cut(s) 578, 672
MslI CAYNNNNRTG 1 cut(s) 30
MspI CCGG 3 cut(s) 218, 240, 270
MspR9I CCNGG 1 cut(s) 270
NciI CCSGG 1 cut(s) 270
NdeII GATC 6 cut(s) 22, 369, 387, 408, 451, 514
NlaIII CATG 4 cut(s) 29, 94, 197, 394
NlaIV GGNNCC 1 cut(s) 453
NmeAIII GCCGAG 1 cut(s) 672
NmuCI GTSAC 1 cut(s) 71
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 682
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 197, 623
PkrI GCNGC 3 cut(s) 293, 466, 538
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PshBI ATTAAT 1 cut(s) 672
PspN4I GGNNCC 1 cut(s) 453
PstNI CAGNNNCTG 1 cut(s) 620
PsuI RGATCY 2 cut(s) 369, 451
RsaI GTAC 2 cut(s) 361, 652
RsaNI GTAC 2 cut(s) 360, 651
RseI CAYNNNNRTG 1 cut(s) 30
SaqAI TTAA 2 cut(s) 578, 672
SatI GCNGC 3 cut(s) 292, 465, 537
Sau3AI GATC 6 cut(s) 22, 369, 387, 408, 451, 514
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 270
SetI ASST 5 cut(s) 19, 289, 546, 569, 639
SfaNI GCATC 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 30
SmlI CTYRAG 2 cut(s) 526, 547
SmoI CTYRAG 2 cut(s) 526, 547
SpeI ACTAGT 1 cut(s) 469
Sse9I AATT 3 cut(s) 67, 305, 311
SsiI CCGC 1 cut(s) 227
SspI AATATT 2 cut(s) 81, 107
SspMI CTAG 2 cut(s) 288, 470
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 3 cut(s) 446, 483, 540
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 1 cut(s) 639
TaqI TCGA 1 cut(s) 189
TasI AATT 3 cut(s) 67, 305, 311
TatI WGTACW 2 cut(s) 359, 650
TfiI GAWTC 2 cut(s) 197, 623
Tru1I TTAA 2 cut(s) 578, 672
Tru9I TTAA 2 cut(s) 578, 672
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 3 cut(s) 291, 464, 536
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 107, 150
VspI ATTAAT 1 cut(s) 672
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 2 cut(s) 288, 470
ZrmI AGTACT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.