pycom05g03490

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014098
Physical Location & Seq
Reverse (-)
4021869 .. 4022609
741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g03490.1

Sequence Viewer

Length: 741 bp
ATGGAAAAGGGTGAATCGAAAGTACTGTCCATATATCACATGGAGTCCTCCTCACCTTCTGATCATGTAAGTGGTGGACGATGCCAAACCCAAACTGTCTCAAAAAATTGTGACACAATATTTCCAGCCATGAAGAAACATCAATATTTTTCTCAAAGAACACCATCATTTTCCTCCTCCTCATCGAGGTCGTCGTCTTCATCTTTCGTATTATCGTCCTGTAATCTCGACCATGATTCTTCTCCTCCTATTAGTCCGGCAACCCCGCTTCCGTTTTCCGGTGTCCCATTCTCTTGGGAACATTTTCCAGGTATTCCAAAGAAACCTAGCAGCAGCAGCAGCAGCAACAACAAGGAATTACAATTACATTCCTCATTGAAGCGTCTCCCACAGCCTCCTCCCACACTCAGTAATCAGAAGATCTCCTCCAAAAAGTTGATCATGGATGATATTATTCAGATCGGACACAACTCCAAAAGGCATACTGCTAATCTCCCAAAGGATCCTTTCTTTGCTGCACTAGTTGAGTGTTCCAAGGACGATGACAGCAACAACAACGACGATGATCAAGGATACTCAAGTGGTGCCGCCAAGGTCTCAAGGAGTGTGGGTGAAAGGTTTGGATTTTTCAATCTTTCTGCATTATCTTGTAAGACAACTTGTGCAGTTTCTGAATCAATCATACACGTTCAAAGGCCGAGTACAACTTCTTATCATCTGATTAATCGCCGTTCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF688

Protein Analysis

247

Amino Acids

26.96

Weight (kDa)

9.14

Isoelectric Point (pI)

69.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014098)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G22680 AT1G71970
fragaria_vesca FvH4_2g11630
malus_domestica MD05G1042300.v1.1
prunus_persica Prupe.8G058900_v2.0.a1
pyrus_communis pycom05g03490 pycom10g03480
rosa_chinensis RchiOBHm_Chr6g0270871
rosa_laevigata RLG00000013763
rosa_multiflora Rmu_sc0010417.1_g000001
rosa_roxburghii Rroxscaffold_3G00238570 Rroxscaffold_7G00197560
rosa_samantha Rh6AG176400 Rh6BG179800 Rh6CG175800 Rh6DG168100
rosa_wichuraiana Rw6G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 584
AciI CCGC 2 cut(s) 266, 588
AclWI GGATC 2 cut(s) 497, 510
AfaI GTAC 2 cut(s) 24, 703
AfiI CCNNNNNNNGG 2 cut(s) 186, 278
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 3 cut(s) 379, 631, 692
AhlI ACTAGT 1 cut(s) 520
AjnI CCWGG 1 cut(s) 307
Alw26I GTCTC 3 cut(s) 103, 389, 601
AlwI GGATC 2 cut(s) 497, 510
AlwNI CAGNNNCTG 1 cut(s) 671
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 6 cut(s) 330, 333, 336, 339, 342, 515
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AseI ATTAAT 1 cut(s) 723
Asp700I GAANNNNTTC 1 cut(s) 303
AsuHPI GGTGA 3 cut(s) 23, 45, 623
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BamHI GGATCC 1 cut(s) 502
BanI GGYRCC 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 6 cut(s) 342, 345, 348, 351, 354, 502
BccI CCATC 1 cut(s) 172
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 1 cut(s) 309
BciVI GTATCC 1 cut(s) 566
BclI TGATCA 3 cut(s) 61, 438, 565
BcoDI GTCTC 3 cut(s) 103, 389, 601
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 2 cut(s) 327, 521
BfuI GTATCC 1 cut(s) 566
BglII AGATCT 1 cut(s) 420
BisI GCNGC 7 cut(s) 331, 334, 337, 340, 343, 516, 588
BlsI GCNGC 7 cut(s) 332, 335, 338, 341, 344, 517, 589
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 504, 586
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpuEI CTTGAG 2 cut(s) 562, 583
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 2 cut(s) 534, 591
BsaWI WCCGGW 1 cut(s) 278
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 278
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 534, 591
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 2 cut(s) 186, 278
BseMII CTCAG 1 cut(s) 421
BseRI GAGGAG 6 cut(s) 40, 166, 169, 234, 387, 415
BseXI GCAGC 6 cut(s) 342, 345, 348, 351, 354, 502
BsgI GTGCAG 2 cut(s) 501, 684
BshFI GGCC 1 cut(s) 697
BshNI GGYRCC 1 cut(s) 584
BsiSI CCGG 2 cut(s) 257, 279
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 2 cut(s) 186, 278
BsmAI GTCTC 3 cut(s) 103, 389, 601
BsmBI CGTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 1 cut(s) 697
Bso31I GGTCTC 1 cut(s) 601
Bsp143I GATC 6 cut(s) 61, 420, 438, 459, 502, 565
BspACI CCGC 2 cut(s) 266, 588
BspANI GGCC 1 cut(s) 697
BspCNI CTCAG 1 cut(s) 420
BspLI GGNNCC 2 cut(s) 504, 586
BspPI GGATC 2 cut(s) 497, 510
BspT107I GGYRCC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 534, 591
BssMI GATC 6 cut(s) 61, 420, 438, 459, 502, 565
BssT1I CCWWGG 2 cut(s) 534, 591
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 2 cut(s) 27, 97
BstDEI CTNAG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 184
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 6 cut(s) 64, 423, 441, 462, 505, 568
BstMAI GTCTC 3 cut(s) 103, 389, 601
BstMBI GATC 6 cut(s) 61, 420, 438, 459, 502, 565
BstMWI GCNNNNNNNGC 3 cut(s) 336, 339, 342
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstV1I GCAGC 6 cut(s) 342, 345, 348, 351, 354, 502
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 420, 502
BstXI CCANNNNNNTGG 1 cut(s) 294
BstYI RGATCY 2 cut(s) 420, 502
BsuI GTATCC 1 cut(s) 566
BsuRI GGCC 1 cut(s) 697
BtsCI GGATG 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 671
CseI GACGC 1 cut(s) 371
Csp6I GTAC 2 cut(s) 23, 702
CspCI CAANNNNNGTGG 2 cut(s) 588, 623
CviAII CATG 5 cut(s) 40, 65, 130, 233, 442
CviJI RGCY 3 cut(s) 128, 394, 697
CviKI_1 RGCY 3 cut(s) 128, 394, 697
CviQI GTAC 2 cut(s) 23, 702
DdeI CTNAG 1 cut(s) 407
DpnI GATC 6 cut(s) 63, 422, 440, 461, 504, 567
DpnII GATC 6 cut(s) 61, 420, 438, 459, 502, 565
Eco130I CCWWGG 2 cut(s) 534, 591
Eco31I GGTCTC 1 cut(s) 601
EcoNI CCTNNNNNAGG 1 cut(s) 184
EcoRII CCWGG 1 cut(s) 307
EcoT14I CCWWGG 2 cut(s) 534, 591
ErhI CCWWGG 2 cut(s) 534, 591
Esp3I CGTCTC 1 cut(s) 389
FaeI CATG 5 cut(s) 43, 68, 133, 236, 445
FaiI YATR 9 cut(s) 32, 34, 41, 66, 131, 234, 443, 483, 683
FaqI GGGAC 1 cut(s) 269
FatI CATG 5 cut(s) 39, 64, 129, 232, 441
FauI CCCGC 1 cut(s) 273
FbaI TGATCA 3 cut(s) 61, 438, 565
Fnu4HI GCNGC 7 cut(s) 331, 334, 337, 340, 343, 516, 588
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 7 cut(s) 331, 334, 337, 340, 343, 516, 588
FspBI CTAG 2 cut(s) 327, 521
GluI GCNGC 7 cut(s) 331, 334, 337, 340, 343, 516, 588
HaeIII GGCC 1 cut(s) 697
HapII CCGG 2 cut(s) 257, 279
HgaI GACGC 1 cut(s) 371
Hin1II CATG 5 cut(s) 43, 68, 133, 236, 445
HinfI GANTC 4 cut(s) 14, 44, 236, 674
HpaII CCGG 2 cut(s) 257, 279
HphI GGTGA 3 cut(s) 23, 45, 623
Hpy166II GTNNAC 1 cut(s) 77
Hpy188I TCNGA 6 cut(s) 61, 417, 459, 464, 673, 720
Hpy188III TCNNGA 2 cut(s) 227, 735
Hpy8I GTNNAC 1 cut(s) 77
Hpy99I CGWCG 2 cut(s) 196, 563
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 27, 97
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 3 cut(s) 518, 641, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 336, 339, 342
HpyF3I CTNAG 1 cut(s) 407
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 5 cut(s) 43, 68, 133, 236, 445
Ksp22I TGATCA 3 cut(s) 61, 438, 565
Kzo9I GATC 6 cut(s) 61, 420, 438, 459, 502, 565
LpnPI CCDG 6 cut(s) 138, 232, 270, 292, 294, 321
Lsp1109I GCAGC 6 cut(s) 342, 345, 348, 351, 354, 502
LweI GCATC 1 cut(s) 71
MaeI CTAG 2 cut(s) 327, 521
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 1 cut(s) 110
MalI GATC 6 cut(s) 63, 422, 440, 461, 504, 567
MboI GATC 6 cut(s) 61, 420, 438, 459, 502, 565
MboII GAAGA 4 cut(s) 145, 189, 231, 430
MflI RGATCY 2 cut(s) 420, 502
MluCI AATT 3 cut(s) 106, 356, 362
MlyI GAGTC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 303
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 1 cut(s) 69
MspI CCGG 2 cut(s) 257, 279
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 3 cut(s) 336, 339, 342
NdeII GATC 6 cut(s) 61, 420, 438, 459, 502, 565
NlaIII CATG 5 cut(s) 43, 68, 133, 236, 445
NlaIV GGNNCC 2 cut(s) 504, 586
NmeAIII GCCGAG 1 cut(s) 723
NmuCI GTSAC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 191, 733
PdmI GAANNNNTTC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 14, 236, 674
PkrI GCNGC 7 cut(s) 332, 335, 338, 341, 344, 517, 589
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PshBI ATTAAT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 2 cut(s) 504, 586
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 2 cut(s) 420, 502
RsaI GTAC 2 cut(s) 24, 703
RsaNI GTAC 2 cut(s) 23, 702
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 7 cut(s) 331, 334, 337, 340, 343, 516, 588
Sau3AI GATC 6 cut(s) 61, 420, 438, 459, 502, 565
ScaI AGTACT 1 cut(s) 24
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 309
SetI ASST 7 cut(s) 58, 191, 313, 328, 597, 620, 690
SfaNI GCATC 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 69
SmlI CTYRAG 2 cut(s) 577, 598
SmoI CTYRAG 2 cut(s) 577, 598
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 3 cut(s) 106, 356, 362
SsiI CCGC 2 cut(s) 266, 588
SspI AATATT 2 cut(s) 120, 146
SspMI CTAG 2 cut(s) 327, 521
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 2 cut(s) 534, 591
TaaI ACNGT 2 cut(s) 27, 97
TaiI ACGT 1 cut(s) 690
TaqI TCGA 3 cut(s) 17, 185, 228
TasI AATT 3 cut(s) 106, 356, 362
TatI WGTACW 2 cut(s) 22, 701
TauI GCSGC 1 cut(s) 590
TfiI GAWTC 3 cut(s) 14, 236, 674
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 6 cut(s) 330, 333, 336, 339, 342, 515
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 146, 189
TspGWI ACGGA 1 cut(s) 261
VspI ATTAAT 1 cut(s) 723
XagI CCTNNNNNAGG 1 cut(s) 184
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 303
XspI CTAG 2 cut(s) 327, 521
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.