MD05G1116900.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011871
Physical Location & Seq
Forward (+)
23804038 .. 23806174
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1116900.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGATTGGCCGGAGAATTTAATTTTGGCAGCAATTCTGGTTTTGTGTGCAGTCATTGATGGAGCTCAGGCAAATACAATGGTGAGTGGCACTGTCTTCTGTGACCAATGCAAAGATGGTGAAATATCCCTCTTTGATTACCCCATCTATGGAGTTAAGGTGCAAGTGGCATGTAGTGACAGTAATGGGCAGATCACAATGTCAAGGGAGGAGACTACAAATTTGTTCGGAAACTATGCCATCAAGTTTGATGGGACACCAGACTTGAGTGGTTGTTATGCTCAGGTTTCTTCAAGCACAGGTAGTGGCTGTGTGGTTAGTGCCGGTCCTGCTCAAAGTCTTAAACTTATGTTTAGGATGTTTGACATGGAGATGTATGCCGTCGACTCTTTGCTCACTCAGCCTGCTCAGCCTATGTCTTTTTGCCCTAAGTCATCTTATAATCCTGTGCCTGCACCCGTAACGCCGGTTAACCCTCCGCCTATACCAGTAGCGCCAACTAACCCTCTGCCTAAACCAGTAACGCCGGCTAGCCCTCCGCCTAAACCAGTGACGCCTTGGATCCCTCCGCCTTTTAGGCTTCCCCCAATGCCTCGATTGCCCCCACTGCCACCACTGCCCTCATTGCCTCCAATGCCACCAATGCCCTTCGTGGAAGCTACAGCATGCCCACATCAGCAGTGGACATTGCGGGAGCACAACTGCTACTGGAGGGCTGTGAACCCGAACACCAAGGTAGCCGTTATTTTCGGACCGCTGGCCGCTAGGAGATATGGGACTGACCTAACATTGTGGCAAGGCCTACAAGGAAGAGGAGACCCTTATAGGACACTCCTAAGGGAAGGGATTACCGCCCTTCTCAACTCCTACAACAGTCTTCAATATCCTTATAATTCAATTGCTGTGGTTCAGCATTTGAATTGGGGCTTGATTGGCTCCAACAAAAATGTCCTCCTCACAGGACTGAGGTTCATTAGGGCTAATTCAGGTTATGGGAAAGTCCCTTGCAAATTTACTGCTTGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

343

Amino Acids

37.25

Weight (kDa)

8.59

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 28 - 107 1.1e-11 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 441, 891
AccI GTMKAC 1 cut(s) 384
AciI CCGC 7 cut(s) 479, 539, 569, 691, 755, 762, 852
AclWI GGATC 2 cut(s) 556, 569
AcoI YGGCCR 2 cut(s) 8, 759
AcsI RAATTY 3 cut(s) 16, 220, 1010
AcyI GRCGYC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 4 cut(s) 294, 881, 897, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 2 cut(s) 65, 659
AluI AGCT 2 cut(s) 65, 659
Alw21I GWGCWC 2 cut(s) 67, 699
Alw26I GTCTC 2 cut(s) 206, 810
AlwI GGATC 2 cut(s) 556, 569
AoxI GGCC 3 cut(s) 8, 759, 799
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 3 cut(s) 16, 220, 1010
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 2 cut(s) 326, 752
AsuHPI GGTGA 2 cut(s) 94, 131
AsuNHI GCTAGC 1 cut(s) 530
AvaII GGWCC 2 cut(s) 326, 752
AxyI CCTNAGG 1 cut(s) 836
BamHI GGATCC 1 cut(s) 561
BanII GRGCYC 1 cut(s) 67
BbsI GAAGAC 2 cut(s) 88, 869
Bbv12I GWGCWC 2 cut(s) 67, 699
BbvI GCAGC 1 cut(s) 41
BccI CCATC 5 cut(s) 53, 110, 152, 245, 248
BceAI ACGGC 2 cut(s) 365, 725
BcoDI GTCTC 2 cut(s) 206, 810
BfaI CTAG 2 cut(s) 531, 765
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 497
BglI GCCNNNNNGGC 1 cut(s) 577
BisI GCNGC 2 cut(s) 30, 762
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 2 cut(s) 31, 763
Bme18I GGWCC 2 cut(s) 326, 752
BmgT120I GGNCC 2 cut(s) 326, 752
BmiI GGNNCC 2 cut(s) 563, 937
BmtI GCTAGC 1 cut(s) 534
BpiI GAAGAC 2 cut(s) 88, 869
BpmI CTGGAG 1 cut(s) 730
Bpu10I CCTNAGC 2 cut(s) 66, 282
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 286
BsaHI GRCGYC 1 cut(s) 554
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 2 cut(s) 557, 732
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse118I RCCGGY 3 cut(s) 323, 466, 526
Bse1I ACTGG 4 cut(s) 488, 518, 548, 713
Bse21I CCTNAGG 1 cut(s) 836
Bse3DI GCAATG 2 cut(s) 623, 686
BseDI CCNNGG 2 cut(s) 557, 732
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 2 cut(s) 623, 686
BseMII CTCAG 5 cut(s) 80, 296, 413, 422, 956
BseNI ACTGG 4 cut(s) 488, 518, 548, 713
BseRI GAGGAG 3 cut(s) 224, 828, 944
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 2 cut(s) 69, 438
BshFI GGCC 3 cut(s) 10, 761, 801
BsiHKAI GWGCWC 2 cut(s) 67, 699
BsiSI CCGG 4 cut(s) 11, 324, 467, 527
BslFI GGGAC 3 cut(s) 268, 790, 986
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 2 cut(s) 206, 810
BsmFI GGGAC 3 cut(s) 268, 790, 986
BsnI GGCC 3 cut(s) 10, 761, 801
Bso31I GGTCTC 1 cut(s) 810
Bsp1286I GDGCHC 2 cut(s) 67, 699
Bsp143I GATC 2 cut(s) 192, 561
Bsp1720I GCTNAGC 1 cut(s) 408
BspACI CCGC 7 cut(s) 479, 539, 569, 691, 755, 762, 852
BspANI GGCC 3 cut(s) 10, 761, 801
BspCNI CTCAG 5 cut(s) 79, 295, 412, 421, 957
BspLI GGNNCC 2 cut(s) 563, 937
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 2 cut(s) 556, 569
BspTNI GGTCTC 1 cut(s) 810
BsrDI GCAATG 2 cut(s) 623, 686
BsrFI RCCGGY 3 cut(s) 323, 466, 526
BsrI ACTGG 4 cut(s) 488, 518, 548, 713
BssAI RCCGGY 3 cut(s) 323, 466, 526
BssECI CCNNGG 2 cut(s) 557, 732
BssMI GATC 2 cut(s) 192, 561
BssNI GRCGYC 1 cut(s) 554
BssT1I CCWWGG 2 cut(s) 557, 732
Bst4CI ACNGT 3 cut(s) 94, 182, 875
Bst6I CTCTTC 1 cut(s) 805
BstACI GRCGYC 1 cut(s) 554
BstC8I GCNNGC 7 cut(s) 405, 453, 528, 532, 667, 759, 1021
BstDEI CTNAG 7 cut(s) 66, 282, 399, 408, 429, 836, 965
BstF5I GGATG 1 cut(s) 363
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 2 cut(s) 195, 564
BstMAI GTCTC 2 cut(s) 206, 810
BstMBI GATC 2 cut(s) 192, 561
BstNSI RCATGY 2 cut(s) 174, 669
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 2 cut(s) 88, 869
BstX2I RGATCY 1 cut(s) 561
BstYI RGATCY 1 cut(s) 561
Bsu36I CCTNAGG 1 cut(s) 836
BsuRI GGCC 3 cut(s) 10, 761, 801
BtsCI GGATG 1 cut(s) 363
BtsI GCAGTG 3 cut(s) 605, 614, 686
BtsIMutI CAGTG 5 cut(s) 90, 555, 605, 614, 686
Cac8I GCNNGC 7 cut(s) 405, 453, 528, 532, 667, 759, 1021
CfoI GCGC 1 cut(s) 496
Cfr10I RCCGGY 3 cut(s) 323, 466, 526
Cfr13I GGNCC 2 cut(s) 326, 752
CpoI CGGWCCG 1 cut(s) 752
CseI GACGC 1 cut(s) 562
CspCI CAANNNNNGTGG 2 cut(s) 885, 920
CspI CGGWCCG 1 cut(s) 752
CviAII CATG 3 cut(s) 171, 367, 666
DdeI CTNAG 7 cut(s) 66, 282, 399, 408, 429, 836, 965
DpnI GATC 2 cut(s) 194, 563
DpnII GATC 2 cut(s) 192, 561
EaeI YGGCCR 2 cut(s) 8, 759
Eam1104I CTCTTC 1 cut(s) 805
EarI CTCTTC 1 cut(s) 805
EciI GGCGGA 3 cut(s) 468, 528, 558
Ecl136II GAGCTC 1 cut(s) 65
Eco130I CCWWGG 2 cut(s) 557, 732
Eco147I AGGCCT 1 cut(s) 801
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 810
Eco47I GGWCC 2 cut(s) 326, 752
Eco53kI GAGCTC 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 65
EcoT14I CCWWGG 2 cut(s) 557, 732
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 557, 732
FaeI CATG 3 cut(s) 174, 370, 669
FaqI GGGAC 3 cut(s) 268, 790, 986
FatI CATG 3 cut(s) 170, 366, 665
FauI CCCGC 1 cut(s) 684
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 2 cut(s) 30, 762
FokI GGATG 1 cut(s) 370
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 2 cut(s) 30, 762
FspBI CTAG 2 cut(s) 531, 765
GlaI GCGC 1 cut(s) 495
GluI GCNGC 2 cut(s) 30, 762
GsuI CTGGAG 1 cut(s) 730
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 3 cut(s) 10, 761, 801
HapII CCGG 4 cut(s) 11, 324, 467, 527
HgaI GACGC 1 cut(s) 562
HhaI GCGC 1 cut(s) 496
Hin1I GRCGYC 1 cut(s) 554
Hin1II CATG 3 cut(s) 174, 370, 669
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HincII GTYRAC 2 cut(s) 385, 472
HindII GTYRAC 2 cut(s) 385, 472
HinfI GANTC 1 cut(s) 386
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 4 cut(s) 11, 324, 467, 527
HphI GGTGA 2 cut(s) 94, 131
Hpy166II GTNNAC 4 cut(s) 385, 472, 684, 721
Hpy188I TCNGA 2 cut(s) 230, 752
Hpy8I GTNNAC 4 cut(s) 385, 472, 684, 721
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 3 cut(s) 658, 836, 866
HpyCH4III ACNGT 3 cut(s) 94, 182, 875
HpyCH4V TGCA 6 cut(s) 50, 111, 163, 455, 1008, 1023
HpyF3I CTNAG 7 cut(s) 66, 282, 399, 408, 429, 836, 965
Hsp92I GRCGYC 1 cut(s) 554
Hsp92II CATG 3 cut(s) 174, 370, 669
HspAI GCGC 1 cut(s) 494
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 2 cut(s) 192, 561
LmnI GCTCC 3 cut(s) 62, 694, 941
Lsp1109I GCAGC 1 cut(s) 41
MaeI CTAG 2 cut(s) 531, 765
MaeIII GTNAC 5 cut(s) 101, 176, 460, 520, 550
MalI GATC 2 cut(s) 194, 563
MboI GATC 2 cut(s) 192, 561
MboII GAAGA 4 cut(s) 88, 282, 822, 869
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 2 cut(s) 67, 699
MluCI AATT 9 cut(s) 16, 21, 33, 220, 892, 897, 919, 982, 1010
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 1 cut(s) 963
MroNI GCCGGC 1 cut(s) 526
MseI TTAA 4 cut(s) 20, 156, 342, 471
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 757
MspI CCGG 4 cut(s) 11, 324, 467, 527
MunI CAATTG 1 cut(s) 897
NaeI GCCGGC 1 cut(s) 528
NdeII GATC 2 cut(s) 192, 561
NgoMIV GCCGGC 1 cut(s) 526
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 3 cut(s) 174, 370, 669
NlaIV GGNNCC 2 cut(s) 563, 937
NmuCI GTSAC 3 cut(s) 101, 176, 550
NspI RCATGY 2 cut(s) 174, 669
PaeI GCATGC 1 cut(s) 669
PceI AGGCCT 1 cut(s) 801
PdiI GCCGGC 1 cut(s) 528
PkrI GCNGC 2 cut(s) 31, 763
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
PsiI TTATAA 2 cut(s) 441, 891
Psp124BI GAGCTC 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 563, 937
PspPI GGNCC 2 cut(s) 326, 752
PsuI RGATCY 1 cut(s) 561
RseI CAYNNNNRTG 1 cut(s) 371
Rsr2I CGGWCCG 1 cut(s) 752
RsrII CGGWCCG 1 cut(s) 752
SacI GAGCTC 1 cut(s) 67
SalI GTCGAC 1 cut(s) 383
SaqAI TTAA 4 cut(s) 20, 156, 342, 471
SatI GCNGC 2 cut(s) 30, 762
Sau3AI GATC 2 cut(s) 192, 561
Sau96I GGNCC 2 cut(s) 326, 752
SchI GAGTC 1 cut(s) 380
SduI GDGCHC 2 cut(s) 67, 699
SetI ASST 9 cut(s) 67, 162, 288, 304, 661, 738, 786, 971, 991
SfcI CTRYAG 1 cut(s) 660
SinI GGWCC 2 cut(s) 326, 752
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 265
SmoI CTYRAG 1 cut(s) 265
SphI GCATGC 1 cut(s) 669
Sse9I AATT 9 cut(s) 16, 21, 33, 220, 892, 897, 919, 982, 1010
SseBI AGGCCT 1 cut(s) 801
SsiI CCGC 7 cut(s) 479, 539, 569, 691, 755, 762, 852
SspMI CTAG 2 cut(s) 531, 765
SstI GAGCTC 1 cut(s) 67
StuI AGGCCT 1 cut(s) 801
StyI CCWWGG 2 cut(s) 557, 732
TaaI ACNGT 3 cut(s) 94, 182, 875
TaqI TCGA 2 cut(s) 384, 595
TasI AATT 9 cut(s) 16, 21, 33, 220, 892, 897, 919, 982, 1010
TauI GCSGC 1 cut(s) 764
Tru1I TTAA 4 cut(s) 20, 156, 342, 471
Tru9I TTAA 4 cut(s) 20, 156, 342, 471
TscAI CASTG 5 cut(s) 97, 555, 612, 621, 686
TseFI GTSAC 3 cut(s) 101, 176, 550
TseI GCWGC 1 cut(s) 29
Tsp45I GTSAC 3 cut(s) 101, 176, 550
TspDTI ATGAA 1 cut(s) 961
TspRI CASTG 5 cut(s) 97, 555, 612, 621, 686
VpaK11BI GGWCC 2 cut(s) 326, 752
XapI RAATTY 3 cut(s) 16, 220, 1010
XceI RCATGY 2 cut(s) 174, 669
XcmI CCANNNNNNNNNTGG 3 cut(s) 113, 555, 678
XmiI GTMKAC 1 cut(s) 384
XspI CTAG 2 cut(s) 531, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.