MD10G1120000.v1.1

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011871
Physical Location & Seq
Forward (+)
19685708 .. 19686466
759 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1120000.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGTGAGTGGCACTGTCTTCTGTGACCAATGCAAAGATGGTGACAGATCCCTCTTTGATTACCCCATCTACGGAGTTAAAGTACAAGTGGCATGTAGTGACAGTAATGGGCAGATCACAATGTCAAGGGAAGAGACTACAAATTGGTTTGGAAACTATGCCATCGGGTTTGATGGGACACCAGATTTGAGCGGCTGTTATGCTCAGATTTCTTCAAGCACAGGGAGTGGCTGTGTGGTTAGTGCTGGTCCTGCTCAAAATCTTAGACTTGTGTTTAGAATGTTTGACATGGGGATGTATGTTGTGGACTCTTTGCTCACTCAGCCTGCTCAGCCCATGTCATTTTGCCCCAAGTCATCTAATCCTGTGCCTGCACCGGTAACGCTGGTTAACCCTCTGCCTAAACCAGTGACGCCGGCAAGCCCTCCGCCTTTTAGGCTCCCCCCCCCAATGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

153

Amino Acids

16.26

Weight (kDa)

4.81

Isoelectric Point (pI)

58.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 2 - 81 4.2e-12 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 192
AciI CCGC 2 cut(s) 192, 428
AclWI GGATC 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 71
AgeI ACCGGT 1 cut(s) 376
AgsI TTSAA 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 128
AlwI GGATC 1 cut(s) 42
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
AsiGI ACCGGT 1 cut(s) 376
AspS9I GGNCC 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 16, 53
AvaII GGWCC 1 cut(s) 248
BbsI GAAGAC 1 cut(s) 10
BccI CCATC 4 cut(s) 32, 74, 167, 170
BcoDI GTCTC 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 436
BisI GCNGC 1 cut(s) 193
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 1 cut(s) 194
Bme18I GGWCC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 1 cut(s) 440
BpiI GAAGAC 1 cut(s) 10
Bpu1102I GCTNAGC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 413
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 217, 247
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse118I RCCGGY 2 cut(s) 376, 415
Bse1I ACTGG 1 cut(s) 407
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMII CTCAG 3 cut(s) 218, 335, 344
BseNI ACTGG 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 357
BshTI ACCGGT 1 cut(s) 376
BsiSI CCGG 2 cut(s) 377, 416
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 71
BsmAI GTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 190
Bsp143I GATC 2 cut(s) 47, 114
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 2 cut(s) 192, 428
BspCNI CTCAG 3 cut(s) 217, 334, 343
BspLI GGNNCC 1 cut(s) 440
BspPI GGATC 1 cut(s) 42
BsrBI CCGCTC 1 cut(s) 192
BsrFI RCCGGY 2 cut(s) 376, 415
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 2 cut(s) 376, 415
BssMI GATC 2 cut(s) 47, 114
BssNI GRCGYC 1 cut(s) 413
Bst4CI ACNGT 2 cut(s) 16, 104
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 327, 372, 417, 421
BstDEI CTNAG 4 cut(s) 204, 263, 321, 330
BstF5I GGATG 1 cut(s) 300
BstKTI GATC 2 cut(s) 50, 117
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 2 cut(s) 47, 114
BstMWI GCNNNNNNNGC 4 cut(s) 251, 322, 331, 436
BstNSI RCATGY 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 10
BstX2I RGATCY 1 cut(s) 47
BstYI RGATCY 1 cut(s) 47
BtsCI GGATG 1 cut(s) 300
BtsIMutI CAGTG 2 cut(s) 12, 414
Cac8I GCNNGC 4 cut(s) 327, 372, 417, 421
Cfr10I RCCGGY 2 cut(s) 376, 415
Cfr13I GGNCC 1 cut(s) 248
CseI GACGC 1 cut(s) 421
Csp6I GTAC 1 cut(s) 83
CspAI ACCGGT 1 cut(s) 376
CviAII CATG 3 cut(s) 93, 289, 337
CviJI RGCY 6 cut(s) 195, 231, 325, 334, 423, 439
CviKI_1 RGCY 6 cut(s) 195, 231, 325, 334, 423, 439
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 4 cut(s) 204, 263, 321, 330
DpnI GATC 2 cut(s) 49, 116
DpnII GATC 2 cut(s) 47, 114
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
EciI GGCGGA 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 248
FaeI CATG 3 cut(s) 96, 292, 340
FaiI YATR 6 cut(s) 94, 159, 201, 290, 300, 338
FaqI GGGAC 1 cut(s) 190
FatI CATG 3 cut(s) 92, 288, 336
Fnu4HI GCNGC 1 cut(s) 193
FokI GGATG 1 cut(s) 307
Fsp4HI GCNGC 1 cut(s) 193
GluI GCNGC 1 cut(s) 193
HapII CCGG 2 cut(s) 377, 416
HgaI GACGC 1 cut(s) 421
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 3 cut(s) 96, 292, 340
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 1 cut(s) 308
HpaI GTTAAC 1 cut(s) 391
HpaII CCGG 2 cut(s) 377, 416
HphI GGTGA 2 cut(s) 16, 53
Hpy166II GTNNAC 2 cut(s) 307, 391
Hpy188I TCNGA 1 cut(s) 207
Hpy8I GTNNAC 2 cut(s) 307, 391
HpyCH4III ACNGT 2 cut(s) 16, 104
HpyCH4V TGCA 2 cut(s) 33, 374
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 322, 331, 436
HpyF3I CTNAG 4 cut(s) 204, 263, 321, 330
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 3 cut(s) 96, 292, 340
KroI GCCGGC 1 cut(s) 415
KroNI GCCGGC 1 cut(s) 417
KspAI GTTAAC 1 cut(s) 391
Kzo9I GATC 2 cut(s) 47, 114
LmnI GCTCC 1 cut(s) 444
MaeIII GTNAC 5 cut(s) 23, 41, 98, 379, 409
MalI GATC 2 cut(s) 49, 116
MbiI CCGCTC 1 cut(s) 192
MboI GATC 2 cut(s) 47, 114
MboII GAAGA 3 cut(s) 10, 143, 204
MflI RGATCY 1 cut(s) 47
MluCI AATT 1 cut(s) 142
MlyI GAGTC 1 cut(s) 302
MnlI CCTC 3 cut(s) 62, 405, 435
MroNI GCCGGC 1 cut(s) 415
MseI TTAA 2 cut(s) 78, 390
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 2 cut(s) 377, 416
MwoI GCNNNNNNNGC 4 cut(s) 251, 322, 331, 436
NaeI GCCGGC 1 cut(s) 417
NdeII GATC 2 cut(s) 47, 114
NgoMIV GCCGGC 1 cut(s) 415
NlaIII CATG 3 cut(s) 96, 292, 340
NlaIV GGNNCC 1 cut(s) 440
NmuCI GTSAC 4 cut(s) 23, 41, 98, 409
NspI RCATGY 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 417
PinAI ACCGGT 1 cut(s) 376
PkrI GCNGC 1 cut(s) 194
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 440
PspPI GGNCC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 47
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 2 cut(s) 78, 390
SatI GCNGC 1 cut(s) 193
Sau3AI GATC 2 cut(s) 47, 114
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 1 cut(s) 302
SinI GGWCC 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 1 cut(s) 142
SsiI CCGC 2 cut(s) 192, 428
TaaI ACNGT 2 cut(s) 16, 104
TasI AATT 1 cut(s) 142
TatI WGTACW 1 cut(s) 82
TauI GCSGC 1 cut(s) 195
Tru1I TTAA 2 cut(s) 78, 390
Tru9I TTAA 2 cut(s) 78, 390
TscAI CASTG 2 cut(s) 19, 414
TseFI GTSAC 4 cut(s) 23, 41, 98, 409
Tsp45I GTSAC 4 cut(s) 23, 41, 98, 409
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 2 cut(s) 19, 414
VpaK11BI GGWCC 1 cut(s) 248
XceI RCATGY 1 cut(s) 96
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.