Rroxscaffold_7G00215510

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011871
Physical Location & Seq
Forward (+)
66136691 .. 66138382
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215510.1

Sequence Viewer

Length: 1032 bp
ATGGGTTCTCTTCAGGTTTTCATTTCTGCAGCAGCATTACTAGTTCTGGTTTTGGCAGCCATTGATGGAGCTCAAGCAAATACCTTGGTCAGTGGCACAGTCTTCTGTGACCAATGCAAAGATGGTGAAAGGTCCCTCTTTGATTATCCAATCTATGGAATTAAAGTACAAGTGGCATGTAGTGACAGTAATGGCCAAGTGACATTGTCAAGGGAGGAGACTACAAACTGGTTTGGGAACTATGCTATGCAATTTGATGGGACACCAGATTTGAGCGGCTGTAATGCCAAGGTTTTAAGCTCAGGGCAAGGCTCATGTGGGATTCCTGCTGGCCCTGCTCAGAGTCTTAAACTTGTGTTTAGGATGTTTGACATGGGAATGTATGCTGTGGACTCTTTGCTCACTCAGCCTGCTCAGCCTATGTCATTTTGCCCGAAGTCATCTAATCCTGTGCCTGCTCCTGTAACACCGGCACCGCCTAGACAAGTAGCACCGGATCCTCCTAGGCAAGTAACACCTGCACCGCCTAGACAAGTAACACCAGCCAGTCCTCCACCTTTCAGGCTTCCCCCAATTGCTCCATTGCCGCCGATGCCTCACTTGCCACCATTGCCTCCACTGCCACCGCTACCACCAATGCCACCAGTGCCCTTCTTGGAACTCACAGCATGCCCACATCAGAGTTGGACAAGGCCAGAGTACAAATGCTACTGGAGGGCAGTGAATCCAGACACAAAGGTAGCTGTTGTTTTCGGATTGCTTGCAGCAAGAAGATATGGGACTGACCTAACATTGTGGCAAGGCCTACAGGGAAGAGGAGACCCTTATAGGACTCTCCTAAGGGAAGGCATCACTGCCTTTCTCAACTCCTACAACAGTCTTCAATTTCCTTACAATACCATTGCTGTTGTGCAGCATATGAATTCGGGATTGATGGGTTCCGAGAGGAATGTCCTTTATACCGCCTTGAGGTTCATCAGGGCTAATTCAGGCTATGGCAAAGTCACCTGCAAATTTACTTCTTGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

343

Amino Acids

37.17

Weight (kDa)

8.9

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 30 - 111 5e-13 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 526, 1016
Acc36I ACCTGC 2 cut(s) 526, 1016
AccB1I GGYRCC 1 cut(s) 472
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 276
AciI CCGC 6 cut(s) 276, 476, 524, 587, 626, 963
AclWI GGATC 2 cut(s) 491, 504
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 2 cut(s) 922, 1013
AfaI GTAC 2 cut(s) 168, 701
AfiI CCNNNNNNNGG 2 cut(s) 155, 969
AgsI TTSAA 1 cut(s) 884
AhlI ACTAGT 1 cut(s) 40
AluBI AGCT 3 cut(s) 71, 300, 743
AluI AGCT 3 cut(s) 71, 300, 743
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 2 cut(s) 212, 813
AlwI GGATC 2 cut(s) 491, 504
AoxI GGCC 4 cut(s) 193, 331, 692, 802
ApeKI GCWGC 5 cut(s) 29, 32, 56, 764, 913
ApoI RAATTY 2 cut(s) 922, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspA2I CCTAGG 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 132, 332
AsuHPI GGTGA 2 cut(s) 137, 997
AvaII GGWCC 1 cut(s) 132
AvrII CCTAGG 1 cut(s) 503
AxyI CCTNAGG 1 cut(s) 839
BaeGI GKGCMC 1 cut(s) 651
BalI TGGCCA 1 cut(s) 195
BamHI GGATCC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 73
BbsI GAAGAC 2 cut(s) 94, 872
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 5 cut(s) 41, 44, 68, 776, 925
BccI CCATC 4 cut(s) 59, 116, 251, 928
BcoDI GTCTC 2 cut(s) 212, 813
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 480, 504, 528
BfmI CTRYAG 2 cut(s) 27, 806
BfuAI ACCTGC 2 cut(s) 526, 1016
BisI GCNGC 7 cut(s) 30, 33, 57, 277, 587, 765, 914
BlnI CCTAGG 1 cut(s) 503
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 7 cut(s) 31, 34, 58, 278, 588, 766, 915
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 132, 332
BmiI GGNNCC 4 cut(s) 134, 474, 498, 940
BmsI GCATC 2 cut(s) 582, 858
BpiI GAAGAC 2 cut(s) 94, 872
BpmI CTGGAG 1 cut(s) 733
Bpu10I CCTNAGC 1 cut(s) 301
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 57, 988
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 3 cut(s) 84, 288, 503
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 969
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 4 cut(s) 233, 546, 644, 716
Bse21I CCTNAGG 1 cut(s) 839
Bse3DI GCAATG 3 cut(s) 581, 608, 900
BseDI CCNNGG 3 cut(s) 84, 288, 503
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 155, 969
BseMI GCAATG 3 cut(s) 581, 608, 900
BseMII CTCAG 4 cut(s) 315, 353, 419, 428
BseNI ACTGG 4 cut(s) 233, 546, 644, 716
BseRI GAGGAG 2 cut(s) 230, 831
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 5 cut(s) 41, 44, 68, 776, 925
BsgI GTGCAG 2 cut(s) 504, 932
BshFI GGCC 4 cut(s) 195, 333, 694, 804
BshNI GGYRCC 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 470, 494
BslFI GGGAC 3 cut(s) 118, 274, 793
BslI CCNNNNNNNGG 2 cut(s) 155, 969
BsmAI GTCTC 2 cut(s) 212, 813
BsmFI GGGAC 3 cut(s) 118, 274, 793
BsnI GGCC 4 cut(s) 195, 333, 694, 804
Bso31I GGTCTC 1 cut(s) 813
Bsp1286I GDGCHC 2 cut(s) 73, 651
Bsp143I GATC 1 cut(s) 496
Bsp1720I GCTNAGC 1 cut(s) 414
BspACI CCGC 6 cut(s) 276, 476, 524, 587, 626, 963
BspANI GGCC 4 cut(s) 195, 333, 694, 804
BspCNI CTCAG 4 cut(s) 314, 352, 418, 427
BspLI GGNNCC 4 cut(s) 134, 474, 498, 940
BspMAI CTGCAG 1 cut(s) 31
BspMI ACCTGC 2 cut(s) 526, 1016
BspPI GGATC 2 cut(s) 491, 504
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 813
BsrBI CCGCTC 1 cut(s) 276
BsrDI GCAATG 3 cut(s) 581, 608, 900
BsrFI RCCGGY 1 cut(s) 469
BsrI ACTGG 4 cut(s) 233, 546, 644, 716
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 84, 288, 503
BssMI GATC 1 cut(s) 496
BssT1I CCWWGG 3 cut(s) 84, 288, 503
Bst4CI ACNGT 3 cut(s) 100, 188, 878
Bst6I CTCTTC 2 cut(s) 15, 808
BstC8I GCNNGC 5 cut(s) 331, 411, 456, 670, 762
BstDEI CTNAG 5 cut(s) 301, 339, 405, 414, 839
BstF5I GGATG 1 cut(s) 369
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 2 cut(s) 212, 813
BstMBI GATC 1 cut(s) 496
BstMWI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
BstNSI RCATGY 2 cut(s) 180, 672
BstSFI CTRYAG 2 cut(s) 27, 806
BstSLI GKGCMC 1 cut(s) 651
BstV1I GCAGC 5 cut(s) 41, 44, 68, 776, 925
BstV2I GAAGAC 2 cut(s) 94, 872
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
Bsu36I CCTNAGG 1 cut(s) 839
BsuRI GGCC 4 cut(s) 195, 333, 694, 804
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 3 cut(s) 617, 726, 852
BtsIMutI CAGTG 5 cut(s) 97, 617, 651, 726, 852
BveI ACCTGC 2 cut(s) 526, 1016
Cac8I GCNNGC 5 cut(s) 331, 411, 456, 670, 762
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 2 cut(s) 132, 332
Csp6I GTAC 2 cut(s) 167, 700
CviAII CATG 4 cut(s) 177, 315, 373, 669
CviQI GTAC 2 cut(s) 167, 700
DdeI CTNAG 5 cut(s) 301, 339, 405, 414, 839
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 15, 808
EarI CTCTTC 2 cut(s) 15, 808
Ecl136II GAGCTC 1 cut(s) 71
Eco130I CCWWGG 3 cut(s) 84, 288, 503
Eco147I AGGCCT 1 cut(s) 804
Eco24I GRGCYC 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 813
Eco47I GGWCC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 71
Eco81I CCTNAGG 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 3 cut(s) 84, 288, 503
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 3 cut(s) 84, 288, 503
FaeI CATG 4 cut(s) 180, 318, 376, 672
FaqI GGGAC 3 cut(s) 118, 274, 793
FatI CATG 4 cut(s) 176, 314, 372, 668
FauNDI CATATG 1 cut(s) 918
Fnu4HI GCNGC 7 cut(s) 30, 33, 57, 277, 587, 765, 914
FokI GGATG 1 cut(s) 376
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 30, 33, 57, 277, 587, 765, 914
FspBI CTAG 4 cut(s) 41, 480, 504, 528
GluI GCNGC 7 cut(s) 30, 33, 57, 277, 587, 765, 914
GsuI CTGGAG 1 cut(s) 733
HaeIII GGCC 4 cut(s) 195, 333, 694, 804
HapII CCGG 2 cut(s) 470, 494
Hin1II CATG 4 cut(s) 180, 318, 376, 672
HinfI GANTC 5 cut(s) 322, 343, 392, 724, 832
HpaII CCGG 2 cut(s) 470, 494
HphI GGTGA 2 cut(s) 137, 997
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 342, 681, 755, 943
Hpy188III TCNNGA 2 cut(s) 728, 927
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 2 cut(s) 661, 839
HpyCH4III ACNGT 3 cut(s) 100, 188, 878
HpyCH4V TGCA 8 cut(s) 29, 117, 250, 521, 764, 913, 1011, 1026
HpyF10VI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
HpyF3I CTNAG 5 cut(s) 301, 339, 405, 414, 839
Hsp92II CATG 4 cut(s) 180, 318, 376, 672
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 3 cut(s) 68, 463, 583
Lsp1109I GCAGC 5 cut(s) 41, 44, 68, 776, 925
LweI GCATC 2 cut(s) 582, 858
MaeI CTAG 4 cut(s) 41, 480, 504, 528
MaeIII GTNAC 7 cut(s) 107, 182, 199, 463, 511, 535, 1003
MalI GATC 1 cut(s) 498
MbiI CCGCTC 1 cut(s) 276
MboI GATC 1 cut(s) 496
MboII GAAGA 4 cut(s) 94, 783, 825, 872
MfeI CAATTG 1 cut(s) 573
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 73, 651
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 7 cut(s) 159, 251, 573, 884, 922, 985, 1013
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 3 cut(s) 352, 386, 826
MmeI TCCRAC 1 cut(s) 665
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
MseI TTAA 3 cut(s) 162, 296, 348
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 195
MspI CCGG 2 cut(s) 470, 494
MunI CAATTG 1 cut(s) 573
MwoI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
NdeI CATATG 1 cut(s) 918
NdeII GATC 1 cut(s) 496
NlaIII CATG 4 cut(s) 180, 318, 376, 672
NlaIV GGNNCC 4 cut(s) 134, 474, 498, 940
NmuCI GTSAC 4 cut(s) 107, 182, 199, 1003
NspI RCATGY 2 cut(s) 180, 672
PaeI GCATGC 1 cut(s) 672
PaqCI CACCTGC 2 cut(s) 526, 1016
PceI AGGCCT 1 cut(s) 804
PfeI GAWTC 2 cut(s) 322, 724
PflFI GACNNNGTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 7 cut(s) 31, 34, 58, 278, 588, 766, 915
PleI GAGTC 3 cut(s) 351, 386, 826
PpsI GAGTC 3 cut(s) 351, 386, 826
PpuMI RGGWCCY 1 cut(s) 132
Psp124BI GAGCTC 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 132
PspN4I GGNNCC 4 cut(s) 134, 474, 498, 940
PspPI GGNCC 2 cut(s) 132, 332
PspPPI RGGWCCY 1 cut(s) 132
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 496
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 2 cut(s) 168, 701
RsaNI GTAC 2 cut(s) 167, 700
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 3 cut(s) 162, 296, 348
SatI GCNGC 7 cut(s) 30, 33, 57, 277, 587, 765, 914
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 2 cut(s) 132, 332
SchI GAGTC 3 cut(s) 352, 386, 826
SduI GDGCHC 2 cut(s) 73, 651
SfaNI GCATC 2 cut(s) 582, 858
SfcI CTRYAG 2 cut(s) 27, 806
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 72, 967
SmoI CTYRAG 2 cut(s) 72, 967
SpeI ACTAGT 1 cut(s) 40
SphI GCATGC 1 cut(s) 672
Sse9I AATT 7 cut(s) 159, 251, 573, 884, 922, 985, 1013
SseBI AGGCCT 1 cut(s) 804
SsiI CCGC 6 cut(s) 276, 476, 524, 587, 626, 963
SspMI CTAG 4 cut(s) 41, 480, 504, 528
SstI GAGCTC 1 cut(s) 73
StuI AGGCCT 1 cut(s) 804
StyI CCWWGG 3 cut(s) 84, 288, 503
TaaI ACNGT 3 cut(s) 100, 188, 878
TasI AATT 7 cut(s) 159, 251, 573, 884, 922, 985, 1013
TatI WGTACW 2 cut(s) 166, 699
TauI GCSGC 2 cut(s) 279, 589
TfiI GAWTC 2 cut(s) 322, 724
Tru1I TTAA 3 cut(s) 162, 296, 348
Tru9I TTAA 3 cut(s) 162, 296, 348
TscAI CASTG 5 cut(s) 97, 624, 651, 726, 859
TseFI GTSAC 4 cut(s) 107, 182, 199, 1003
TseI GCWGC 5 cut(s) 29, 32, 56, 764, 913
Tsp45I GTSAC 4 cut(s) 107, 182, 199, 1003
TspDTI ATGAA 3 cut(s) 10, 935, 964
TspRI CASTG 5 cut(s) 97, 624, 651, 726, 859
Tth111I GACNNNGTC 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 2 cut(s) 922, 1013
XceI RCATGY 2 cut(s) 180, 672
XcmI CCANNNNNNNNNTGG 2 cut(s) 119, 681
XmaJI CCTAGG 1 cut(s) 503
XspI CTAG 4 cut(s) 41, 480, 504, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.