RchiOBHm_Chr6g0244771

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011871
Physical Location & Seq
Reverse (-)
760203 .. 762121
1919 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21935

Sequence Viewer

Length: 1032 bp
ATGGGTTCTCTTCAGGTTTTCATTTCTGCAGCAGCATTACTAGTTCTGGTTTTGGCAGCCATTGATGAAGCTCAAGCAAATACCGTGGTCAGTGGCACAGTCTTCTGTGACCAATGCAAAGATGGTGAAAGGTCCCTCTTTGATTATCCAATCTATGGAATTAAAGTACAAGTGGCATGTAGTGACAGTAATGGCCAAGTGACAATGTCAAGGGAGGAGACTACCAATTGGTTTGGGAACTATGCTATGCAATTTGATGGGACACCAGATTTGAGCGGCTGTTATGCCAAGGTTTTAAGCTCAGGGCAAGGCTCATGTGGGATTCCTGCTGGCCCTGCTCAGAGTCTTAAACTTGTGTTTAGGATGTTTGGCATGGGAATGTATGCTGTGGACTCTTTGCTCACTCAGCCTGCTCAGCCTATGTCATTTTGCCCAAAGTCATCTAATCCTGTGCCTGCTCCTGTAACACCAGCGCCGCCTAGACAAGTAGCACCGGCTCCTCCTAGGCAAGTAACACCGGCACCGCCTAGACAAGTAACGCCAGCCAGTCCTCCTCCTTTCAGGCTTCCCCCAATTGCTCCATTGCCGCCAATGCCTCACTTGCCACCATTGCCGCCACTGCCACCGCTACCACCAATGCCACCAATGCCCTTCTTGGAACTCACAGCATGCCCACATCAGAGTTGGACAAGGCCAGAGTACAAATGCTACTGGAGGGCAGTGAATCCAGACACAAAGGTTGCTGTTGTTTTCGGATTGCTTGCAGCAAGAAGATATGGGACTGACCTAACATTGTGGCAAGGCCTACAGGGAAGAGGAGACCCTTATAGGACTCTCCTAAGGGAAGGCATCACTGCCTTTCTCAACTCCTACAACAGTCTTCAATTTCCTTACAATACCATTGCTGTTGTGCAGCATATGAATTCTGGATTGATGGGTTCCGAGAGGAATGTCCTCTATACAGCCTTGAGGTTCATCAGGGCTAATTCAGGCTATGGCAAAGTCACCTGCAAATTTACTTCTTGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

343

Amino Acids

37.22

Weight (kDa)

8.99

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 30 - 111 6.6e-13 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1016
Acc36I ACCTGC 1 cut(s) 1016
AccB1I GGYRCC 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 276
AciI CCGC 6 cut(s) 276, 476, 524, 587, 614, 626
AcoI YGGCCR 1 cut(s) 193
AcsI RAATTY 2 cut(s) 922, 1013
AfaI GTAC 2 cut(s) 168, 701
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 1 cut(s) 884
AhlI ACTAGT 1 cut(s) 40
AluBI AGCT 2 cut(s) 71, 300
AluI AGCT 2 cut(s) 71, 300
Alw26I GTCTC 2 cut(s) 212, 813
AoxI GGCC 4 cut(s) 193, 331, 692, 802
ApeKI GCWGC 5 cut(s) 29, 32, 56, 764, 913
ApoI RAATTY 2 cut(s) 922, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspA2I CCTAGG 1 cut(s) 503
AspLEI GCGC 1 cut(s) 475
AspS9I GGNCC 2 cut(s) 132, 332
AsuHPI GGTGA 2 cut(s) 137, 997
AvaII GGWCC 1 cut(s) 132
AvrII CCTAGG 1 cut(s) 503
AxyI CCTNAGG 1 cut(s) 839
BalI TGGCCA 1 cut(s) 195
BanI GGYRCC 1 cut(s) 520
BbsI GAAGAC 2 cut(s) 94, 872
BbvI GCAGC 5 cut(s) 41, 44, 68, 776, 925
BccI CCATC 3 cut(s) 116, 251, 928
BcoDI GTCTC 2 cut(s) 212, 813
BcuI ACTAGT 1 cut(s) 40
BfaI CTAG 4 cut(s) 41, 480, 504, 528
BfmI CTRYAG 2 cut(s) 27, 806
BfoI RGCGCY 1 cut(s) 476
BfuAI ACCTGC 1 cut(s) 1016
BisI GCNGC 9 cut(s) 30, 33, 57, 277, 476, 587, 614, 765, 914
BlnI CCTAGG 1 cut(s) 503
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 9 cut(s) 31, 34, 58, 278, 477, 588, 615, 766, 915
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 132, 332
BmiI GGNNCC 4 cut(s) 134, 498, 522, 940
BmsI GCATC 1 cut(s) 858
BpiI GAAGAC 2 cut(s) 94, 872
BpmI CTGGAG 1 cut(s) 733
Bpu10I CCTNAGC 1 cut(s) 301
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 57, 988
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 3 cut(s) 84, 288, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 2 cut(s) 493, 517
Bse1I ACTGG 2 cut(s) 546, 716
Bse21I CCTNAGG 1 cut(s) 839
Bse3DI GCAATG 3 cut(s) 581, 608, 900
BseDI CCNNGG 3 cut(s) 84, 288, 503
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 3 cut(s) 581, 608, 900
BseMII CTCAG 4 cut(s) 315, 353, 419, 428
BseNI ACTGG 2 cut(s) 546, 716
BseRI GAGGAG 4 cut(s) 230, 489, 543, 831
BseXI GCAGC 5 cut(s) 41, 44, 68, 776, 925
BsgI GTGCAG 1 cut(s) 932
BshFI GGCC 4 cut(s) 195, 333, 694, 804
BshNI GGYRCC 1 cut(s) 520
BsiSI CCGG 2 cut(s) 494, 518
BslFI GGGAC 3 cut(s) 118, 274, 793
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 212, 813
BsmFI GGGAC 3 cut(s) 118, 274, 793
BsnI GGCC 4 cut(s) 195, 333, 694, 804
Bso31I GGTCTC 1 cut(s) 813
Bsp1720I GCTNAGC 1 cut(s) 414
BspACI CCGC 6 cut(s) 276, 476, 524, 587, 614, 626
BspANI GGCC 4 cut(s) 195, 333, 694, 804
BspCNI CTCAG 4 cut(s) 314, 352, 418, 427
BspLI GGNNCC 4 cut(s) 134, 498, 522, 940
BspMAI CTGCAG 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 1016
BspT107I GGYRCC 1 cut(s) 520
BspTNI GGTCTC 1 cut(s) 813
BsrBI CCGCTC 1 cut(s) 276
BsrDI GCAATG 3 cut(s) 581, 608, 900
BsrFI RCCGGY 2 cut(s) 493, 517
BsrI ACTGG 2 cut(s) 546, 716
BssAI RCCGGY 2 cut(s) 493, 517
BssECI CCNNGG 3 cut(s) 84, 288, 503
BssT1I CCWWGG 2 cut(s) 288, 503
Bst4CI ACNGT 4 cut(s) 85, 100, 188, 878
Bst6I CTCTTC 2 cut(s) 15, 808
BstC8I GCNNGC 6 cut(s) 331, 411, 456, 543, 670, 762
BstDEI CTNAG 5 cut(s) 301, 339, 405, 414, 839
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 476
BstHHI GCGC 1 cut(s) 475
BstMAI GTCTC 2 cut(s) 212, 813
BstMWI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
BstNSI RCATGY 2 cut(s) 180, 672
BstSFI CTRYAG 2 cut(s) 27, 806
BstV1I GCAGC 5 cut(s) 41, 44, 68, 776, 925
BstV2I GAAGAC 2 cut(s) 94, 872
Bsu36I CCTNAGG 1 cut(s) 839
BsuRI GGCC 4 cut(s) 195, 333, 694, 804
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 3 cut(s) 617, 726, 852
BtsIMutI CAGTG 4 cut(s) 97, 617, 726, 852
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 6 cut(s) 331, 411, 456, 543, 670, 762
CfoI GCGC 1 cut(s) 475
Cfr10I RCCGGY 2 cut(s) 493, 517
Cfr13I GGNCC 2 cut(s) 132, 332
Csp6I GTAC 2 cut(s) 167, 700
CspCI CAANNNNNGTGG 2 cut(s) 66, 101
CviAII CATG 4 cut(s) 177, 315, 373, 669
CviQI GTAC 2 cut(s) 167, 700
DdeI CTNAG 5 cut(s) 301, 339, 405, 414, 839
EaeI YGGCCR 1 cut(s) 193
Eam1104I CTCTTC 2 cut(s) 15, 808
EarI CTCTTC 2 cut(s) 15, 808
Eco130I CCWWGG 2 cut(s) 288, 503
Eco147I AGGCCT 1 cut(s) 804
Eco31I GGTCTC 1 cut(s) 813
Eco47I GGWCC 1 cut(s) 132
Eco81I CCTNAGG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 922
EcoT14I CCWWGG 2 cut(s) 288, 503
ErhI CCWWGG 2 cut(s) 288, 503
FaeI CATG 4 cut(s) 180, 318, 376, 672
FaqI GGGAC 3 cut(s) 118, 274, 793
FatI CATG 4 cut(s) 176, 314, 372, 668
FauNDI CATATG 1 cut(s) 918
Fnu4HI GCNGC 9 cut(s) 30, 33, 57, 277, 476, 587, 614, 765, 914
FokI GGATG 1 cut(s) 376
Fsp4HI GCNGC 9 cut(s) 30, 33, 57, 277, 476, 587, 614, 765, 914
FspBI CTAG 4 cut(s) 41, 480, 504, 528
GlaI GCGC 1 cut(s) 474
GluI GCNGC 9 cut(s) 30, 33, 57, 277, 476, 587, 614, 765, 914
GsuI CTGGAG 1 cut(s) 733
HaeII RGCGCY 1 cut(s) 476
HaeIII GGCC 4 cut(s) 195, 333, 694, 804
HapII CCGG 2 cut(s) 494, 518
HhaI GCGC 1 cut(s) 475
Hin1II CATG 4 cut(s) 180, 318, 376, 672
Hin6I GCGC 1 cut(s) 473
HinP1I GCGC 1 cut(s) 473
HinfI GANTC 5 cut(s) 322, 343, 392, 724, 832
HpaII CCGG 2 cut(s) 494, 518
HphI GGTGA 2 cut(s) 137, 997
Hpy166II GTNNAC 1 cut(s) 391
Hpy188I TCNGA 4 cut(s) 342, 681, 755, 943
Hpy188III TCNNGA 2 cut(s) 728, 927
Hpy8I GTNNAC 1 cut(s) 391
HpyAV CCTTC 2 cut(s) 661, 839
HpyCH4III ACNGT 4 cut(s) 85, 100, 188, 878
HpyCH4V TGCA 7 cut(s) 29, 117, 250, 764, 913, 1011, 1026
HpyF10VI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
HpyF3I CTNAG 5 cut(s) 301, 339, 405, 414, 839
Hsp92II CATG 4 cut(s) 180, 318, 376, 672
HspAI GCGC 1 cut(s) 473
LmnI GCTCC 3 cut(s) 463, 502, 583
Lsp1109I GCAGC 5 cut(s) 41, 44, 68, 776, 925
LweI GCATC 1 cut(s) 858
MaeI CTAG 4 cut(s) 41, 480, 504, 528
MaeIII GTNAC 7 cut(s) 107, 182, 199, 463, 511, 535, 1003
MbiI CCGCTC 1 cut(s) 276
MboII GAAGA 4 cut(s) 94, 783, 825, 872
MfeI CAATTG 2 cut(s) 226, 573
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 8 cut(s) 159, 226, 251, 573, 884, 922, 985, 1013
MluNI TGGCCA 1 cut(s) 195
MlyI GAGTC 3 cut(s) 352, 386, 826
MmeI TCCRAC 1 cut(s) 665
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
MseI TTAA 3 cut(s) 162, 296, 348
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 195
MspI CCGG 2 cut(s) 494, 518
MunI CAATTG 2 cut(s) 226, 573
MwoI GCNNNNNNNGC 8 cut(s) 335, 406, 415, 592, 601, 610, 619, 646
NdeI CATATG 1 cut(s) 918
NlaIII CATG 4 cut(s) 180, 318, 376, 672
NlaIV GGNNCC 4 cut(s) 134, 498, 522, 940
NmuCI GTSAC 4 cut(s) 107, 182, 199, 1003
NspI RCATGY 2 cut(s) 180, 672
PaeI GCATGC 1 cut(s) 672
PaqCI CACCTGC 1 cut(s) 1016
PceI AGGCCT 1 cut(s) 804
PfeI GAWTC 2 cut(s) 322, 724
PflFI GACNNNGTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 9 cut(s) 31, 34, 58, 278, 477, 588, 615, 766, 915
PleI GAGTC 3 cut(s) 351, 386, 826
PpsI GAGTC 3 cut(s) 351, 386, 826
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
PspN4I GGNNCC 4 cut(s) 134, 498, 522, 940
PspPI GGNCC 2 cut(s) 132, 332
PspPPI RGGWCCY 1 cut(s) 132
PstI CTGCAG 1 cut(s) 31
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 2 cut(s) 168, 701
RsaNI GTAC 2 cut(s) 167, 700
RseI CAYNNNNRTG 1 cut(s) 377
SaqAI TTAA 3 cut(s) 162, 296, 348
SatI GCNGC 9 cut(s) 30, 33, 57, 277, 476, 587, 614, 765, 914
Sau96I GGNCC 2 cut(s) 132, 332
SchI GAGTC 3 cut(s) 352, 386, 826
SetI ASST 9 cut(s) 18, 73, 134, 294, 302, 741, 789, 974, 1010
SfaNI GCATC 1 cut(s) 858
SfcI CTRYAG 2 cut(s) 27, 806
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 72, 967
SmoI CTYRAG 2 cut(s) 72, 967
SpeI ACTAGT 1 cut(s) 40
SphI GCATGC 1 cut(s) 672
Sse9I AATT 8 cut(s) 159, 226, 251, 573, 884, 922, 985, 1013
SseBI AGGCCT 1 cut(s) 804
SsiI CCGC 6 cut(s) 276, 476, 524, 587, 614, 626
SspMI CTAG 4 cut(s) 41, 480, 504, 528
StuI AGGCCT 1 cut(s) 804
StyI CCWWGG 2 cut(s) 288, 503
TaaI ACNGT 4 cut(s) 85, 100, 188, 878
TasI AATT 8 cut(s) 159, 226, 251, 573, 884, 922, 985, 1013
TatI WGTACW 2 cut(s) 166, 699
TauI GCSGC 4 cut(s) 279, 478, 589, 616
TfiI GAWTC 2 cut(s) 322, 724
Tru1I TTAA 3 cut(s) 162, 296, 348
Tru9I TTAA 3 cut(s) 162, 296, 348
TscAI CASTG 4 cut(s) 97, 624, 726, 859
TseFI GTSAC 4 cut(s) 107, 182, 199, 1003
TseI GCWGC 5 cut(s) 29, 32, 56, 764, 913
Tsp45I GTSAC 4 cut(s) 107, 182, 199, 1003
TspDTI ATGAA 4 cut(s) 10, 81, 935, 964
TspRI CASTG 4 cut(s) 97, 624, 726, 859
Tth111I GACNNNGTC 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 155
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 2 cut(s) 922, 1013
XceI RCATGY 2 cut(s) 180, 672
XcmI CCANNNNNNNNNTGG 2 cut(s) 119, 681
XmaJI CCTAGG 1 cut(s) 503
XspI CTAG 4 cut(s) 41, 480, 504, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.