Rorug05G0510500

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011871
Physical Location & Seq
Forward (+)
69447578 .. 69455455
7878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0510500.1

Sequence Viewer

Length: 522 bp
ATGGGAACCCTAAACGACCACGTGTACATGTGCTACGAGACACACGAGACTGCCAAGTCCCTCATGGACGCTCTCACCGCCACTTACACCCAACCCTCGATGACGAAGCGGATGTACAAGCTCCGCAACTACGTGGGGCACAAGATGGCCGAGGGCAAATCCGTCCATGAGCATATTATGGAGATGGGCTCGATGGCTTATGATCTCCAATGCGAGGGGTTGAAGATTCCGGAGGAAGTTCAGGCTGTGATGCTGATGAATAGCGTGCCGGAGAGTTGGGACGAGATGATGACCGTGGTGAGGTTGAACATGGATTTCGATAAGTCTAAGTCGTCGGGTGAGCCAGATTTCGGTTTGCACAAGGTGAGCAGCAGGCTCAGGGAGATCGGGGCTTTGAAAAAGTTGTATCGGAGGCAGGAGGAGGAGGAGGCCAAGCAGAGGAGGCCGCATTTCAATGGCCATTGCCATACTTGTGGAGAATATGGGCATCACCGTAATCACTGCACCATTGCCATGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

173

Amino Acids

20.06

Weight (kDa)

6.86

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 4 - 101 7.9e-17 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 229
AciI CCGC 4 cut(s) 78, 109, 124, 446
AcoI YGGCCR 2 cut(s) 147, 457
AcvI CACGTG 1 cut(s) 22
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 2 cut(s) 26, 116
AfiI CCNNNNNNNGG 4 cut(s) 214, 300, 350, 438
AflIII ACRYGT 2 cut(s) 21, 27
AgsI TTSAA 4 cut(s) 223, 307, 397, 454
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 32, 41
Aor13HI TCCGGA 1 cut(s) 229
AoxI GGCC 4 cut(s) 147, 429, 443, 457
ApeKI GCWGC 1 cut(s) 369
AsuHPI GGTGA 5 cut(s) 67, 310, 350, 376, 482
BaeGI GKGCMC 1 cut(s) 141
BalI TGGCCA 1 cut(s) 459
BanII GRGCYC 1 cut(s) 191
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 1 cut(s) 44
BbrPI CACGTG 1 cut(s) 22
BbvI GCAGC 1 cut(s) 381
BccI CCATC 3 cut(s) 139, 178, 187
BcoDI GTCTC 2 cut(s) 32, 41
BisI GCNGC 2 cut(s) 370, 446
BlsI GCNGC 2 cut(s) 371, 447
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 2 cut(s) 240, 496
BplI GAGNNNNNCTC 2 cut(s) 173, 205
Bpu10I CCTNAGC 1 cut(s) 377
BsaAI YACGTR 2 cut(s) 22, 133
BsaJI CCNNGG 2 cut(s) 150, 294
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 4 cut(s) 214, 300, 350, 438
Bse3DI GCAATG 2 cut(s) 460, 507
BseAI TCCGGA 1 cut(s) 229
BseDI CCNNGG 2 cut(s) 150, 294
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 4 cut(s) 214, 300, 350, 438
BseMI GCAATG 2 cut(s) 460, 507
BseMII CTCAG 1 cut(s) 391
BseRI GAGGAG 4 cut(s) 434, 437, 440, 454
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 1 cut(s) 381
BsgI GTGCAG 1 cut(s) 487
BshFI GGCC 4 cut(s) 149, 431, 445, 459
BsiSI CCGG 2 cut(s) 230, 269
BslFI GGGAC 2 cut(s) 43, 293
BslI CCNNNNNNNGG 4 cut(s) 214, 300, 350, 438
BsmAI GTCTC 2 cut(s) 32, 41
BsmFI GGGAC 2 cut(s) 43, 293
BsnI GGCC 4 cut(s) 149, 431, 445, 459
Bsp1286I GDGCHC 2 cut(s) 141, 191
Bsp13I TCCGGA 1 cut(s) 229
Bsp1407I TGTACA 2 cut(s) 24, 114
Bsp143I GATC 2 cut(s) 202, 384
BspACI CCGC 4 cut(s) 78, 109, 124, 446
BspANI GGCC 4 cut(s) 149, 431, 445, 459
BspCNI CTCAG 1 cut(s) 390
BspEI TCCGGA 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 7
BsrDI GCAATG 2 cut(s) 460, 507
BsrGI TGTACA 2 cut(s) 24, 114
BssECI CCNNGG 2 cut(s) 150, 294
BssMI GATC 2 cut(s) 202, 384
BssSI CACGAG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 295, 494
BstAUI TGTACA 2 cut(s) 24, 114
BstBAI YACGTR 2 cut(s) 22, 133
BstC8I GCNNGC 2 cut(s) 266, 374
BstDEI CTNAG 2 cut(s) 327, 377
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 1 cut(s) 117
BstKTI GATC 2 cut(s) 205, 387
BstMAI GTCTC 2 cut(s) 32, 41
BstMBI GATC 2 cut(s) 202, 384
BstMWI GCNNNNNNNGC 2 cut(s) 77, 442
BstNSI RCATGY 1 cut(s) 31
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 381
BstXI CCANNNNNNTGG 1 cut(s) 473
BsuRI GGCC 4 cut(s) 149, 431, 445, 459
BtgI CCRYGG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 117
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 266, 374
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 25, 115
CviAII CATG 5 cut(s) 28, 64, 167, 310, 514
CviQI GTAC 2 cut(s) 25, 115
DdeI CTNAG 2 cut(s) 327, 377
DpnI GATC 2 cut(s) 204, 386
DpnII GATC 2 cut(s) 202, 384
DraIII CACNNNGTG 1 cut(s) 364
DrdI GACNNNNNNGTC 1 cut(s) 55
DseDI GACNNNNNNGTC 1 cut(s) 55
EaeI YGGCCR 2 cut(s) 147, 457
Eco24I GRGCYC 1 cut(s) 191
Eco72I CACGTG 1 cut(s) 22
EcoT38I GRGCYC 1 cut(s) 191
FaeI CATG 5 cut(s) 31, 67, 170, 313, 517
FaqI GGGAC 2 cut(s) 43, 293
FatI CATG 5 cut(s) 27, 63, 166, 309, 513
Fnu4HI GCNGC 2 cut(s) 370, 446
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 370, 446
GluI GCNGC 2 cut(s) 370, 446
HaeIII GGCC 4 cut(s) 149, 431, 445, 459
HapII CCGG 2 cut(s) 230, 269
HgaI GACGC 1 cut(s) 77
Hin1II CATG 5 cut(s) 31, 67, 170, 313, 517
HinfI GANTC 1 cut(s) 226
HpaII CCGG 2 cut(s) 230, 269
HphI GGTGA 5 cut(s) 67, 310, 350, 376, 482
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 1 cut(s) 411
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 1 cut(s) 25
Hpy99I CGWCG 1 cut(s) 337
HpyCH4III ACNGT 2 cut(s) 295, 494
HpyCH4IV ACGT 2 cut(s) 21, 132
HpyCH4V TGCA 2 cut(s) 358, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 442
HpyF3I CTNAG 2 cut(s) 327, 377
HpySE526I ACGT 2 cut(s) 21, 132
Hsp92II CATG 5 cut(s) 31, 67, 170, 313, 517
Kpn2I TCCGGA 1 cut(s) 229
Kzo9I GATC 2 cut(s) 202, 384
LmnI GCTCC 1 cut(s) 126
LpnPI CCDG 7 cut(s) 227, 243, 282, 357, 358, 364, 401
Lsp1109I GCAGC 1 cut(s) 381
LweI GCATC 2 cut(s) 240, 496
MaeII ACGT 2 cut(s) 21, 132
MalI GATC 2 cut(s) 204, 386
MboI GATC 2 cut(s) 202, 384
MboII GAAGA 1 cut(s) 235
MhlI GDGCHC 2 cut(s) 141, 191
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
Mox20I TGGCCA 1 cut(s) 459
MroI TCCGGA 1 cut(s) 229
MscI TGGCCA 1 cut(s) 459
MslI CAYNNNNRTG 3 cut(s) 453, 471, 512
Msp20I TGGCCA 1 cut(s) 459
MspI CCGG 2 cut(s) 230, 269
MwoI GCNNNNNNNGC 2 cut(s) 77, 442
NdeII GATC 2 cut(s) 202, 384
NlaIII CATG 5 cut(s) 31, 67, 170, 313, 517
NlaIV GGNNCC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 175
NspI RCATGY 1 cut(s) 31
PciI ACATGT 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 2 cut(s) 371, 447
PmaCI CACGTG 1 cut(s) 22
PmlI CACGTG 1 cut(s) 22
Ppu21I YACGTR 2 cut(s) 22, 133
PscI ACATGT 1 cut(s) 27
PspCI CACGTG 1 cut(s) 22
PspN4I GGNNCC 1 cut(s) 7
RsaI GTAC 2 cut(s) 26, 116
RsaNI GTAC 2 cut(s) 25, 115
RseI CAYNNNNRTG 3 cut(s) 453, 471, 512
SatI GCNGC 2 cut(s) 370, 446
Sau3AI GATC 2 cut(s) 202, 384
SduI GDGCHC 2 cut(s) 141, 191
SetI ASST 5 cut(s) 24, 123, 135, 305, 366
SfaNI GCATC 2 cut(s) 240, 496
SmiMI CAYNNNNRTG 3 cut(s) 453, 471, 512
SsiI CCGC 4 cut(s) 78, 109, 124, 446
TaaI ACNGT 2 cut(s) 295, 494
TaiI ACGT 2 cut(s) 24, 135
TaqI TCGA 3 cut(s) 98, 191, 318
TatI WGTACW 2 cut(s) 24, 114
TauI GCSGC 1 cut(s) 448
TfiI GAWTC 1 cut(s) 226
TscAI CASTG 1 cut(s) 506
TseI GCWGC 1 cut(s) 369
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 1 cut(s) 506
XceI RCATGY 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.