Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008941

Physical Location & Seq

Genomic Coordinates Forward (+)

27573075 .. 27577814

Gene Structure 4740 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1144100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3396 bp

ATGTCAACTTCCCAGAATGGAGAGCCGTCAAAGAAGGCGATAAAAACCCCAGGTGGTTCTGGTAGCTCTCAAGACAAAGCTAATTTGAGTGGCCAAACTGTTAAGTTTGCGCGAAGAACTTCAAGTGGACGGTATGTGAATCTGTCGAGAGAAGACCTTGATATGTCCGACGAATTATCCGGGGACTATATGAACTACACAGTTCATATTCCTCCCACCCCGGATAACCAGCCCATGGACACATCCGTAGCTGTCAAGGCAGAGGAGCAATATGTTTCAAATTCCTTGTTTACCGGAGGGTTCAATAGCGTGACTCGTGCGCATCTCATGGATAAGGTGATTGATTCAGAGGTGACTCATCCTCAGATGGCTGGAGCCAAAGGCTCTGCATGCATGATGCCTTCTTGTGATGGTAAAGTGATGAAGGATGAGAGAGGAGTTGATATAACCCCTTGTGATTGCCGGTTCAAAATCTGTAGAGATTGTTATCTGGATGCACAGAACGACACTGGCCTTTGTCCGGGCTGCAAGGAGCAATACAAAGTAGGAGACGATTATGATGAGCCATCAGATTACAACAGTGGAACGCTGCAATTGCCTGGTCCTGACGGAAAAAGGGATAACATGTCTGTGATGAAGAGGAACCAAACGGGAGAATTTGATCACAATAGGTGGTTGTTTGAGACCAACGGGACGTATGGCATTGGCAATGCTTTCTATCCCAAAGATGACGGGTATGGTGATGGAGGTGGTGATTGTTTTGCAGGGGGCTCGCTGGATGCAGATGACAAGCCTTGGAAGCCCCTCAGCAGGGTATTGCCGATCCCAGCTGCCATTATCAGCCCCTACAGGTTACTGATCTTCGTTCGATTAATCGTGCTATGTTTGTTCCTGCATTGGAGAATAGTCAATCCAAACAACGATGCAAGATGGCTGTGGCTCATGTCGATTATCTGCGAAATATGGTTCGCCTTCGCTTGGATTCTTGATCAGACTCCTAAGTTTTTCCCCATTAATCGTCTGACCGATCTTGAAGTCCTCCACGACAAGTTTGACATGCCAACACCATCCAATCCAATGGGCCGGTCTGACCTCCCTGGCATTGACATCTTTGTATCCACTGCTGATCCTGACGTGGAGCCACCTCTCACCACTGCCAACACCATCCTTTCAATCCTAGCCGTTGATTACCCGGTTGAAAAGATAGCTTGCTACGTCTCTGATGATGGAGCTGCCCTCCTCACCTTCGAGGCAATGGCGGAGGCTGCTAGTTTCGCGGACTTGTGGGTCCCCTTCTGCCGGAAGCACAACATTGAGCCGAGGAATCCTGACAGTTACTTCGCGAGGAAAGTTGACCCAACAAAGAACAAGAGTAGTCTGGACTTTGTGAAGGATAGGAGGAAGATCAAGAGGGAGTATGATGAGTTCAAGGTGAGGATCAACGGGCTTCCCGATTCGATCAGGAGGCGGTCCGATGCTTTCCATGCCAGGGAGGAAATGAAGCAGTTGAAGCATATGAGGGAGAATGCAACTGACCCTCTGGAGCAAGTCAAAGTCACCAGGGCTACATGGATGGCTGATGGCACACACTGGCCTGGTGCTTGGGCTGTTCCTTCCCATGACCATGCCAAAGCTGACCATTCAGCAGTTCTTCAGGTGATGTTGAAGCCTCCTAGTCCTGACCCCCTATTGGGAAGTGCCGATGATGACAAGCTCATAGATTTCACAGATGTGGATATACGCCTCCCAATGTTTGTCTACATGTCGCGAGAAAAGCGGCCAGGCTATGATCCCAACAAGAAAGCTGGCGCCATGAATGCGCTGGTGAGAGCATCCGCCATCTTGTCGAACGGCCCTTTTATTCTCAACCTTGACTGTGATCACTACATCAACAACTGCAAAGCTATCCGTGAAGGGATGTGCTTCATGATGGACAGAGGCGGTGAAAACATCTGCTACATTCAGTTTCCTCAAAGATTCGATGGCATCGATCCCTCTGATCGCTATGCCAATCACAACACCGTGTTTTTCGATGGCACAATGCGCGCGCTTGATGGTTTGCAGGGTCCGCTGTATGTGGGAACCGGGACCATGTTCCGGCGTTTTGCGTTGTACGGTTTTGATCCACCAAATTCTAAGAAATTGCCAGTGAAGAAGGATGCCGTGAAACAAGGAGAGCCTTTGACACAGTCGAATACCCAACCTTTGACAGCCAATGACTTCGATCCAGATCTTGACACCAATCTACTTCCAAAGCGTTTTGGAAATTCGAAAATGCTGGCGGAATCCATACCAGTTGCTGAGTACCAAGGACGACCCCTAGCTGATCATCCTGCAGTGAAATTTGGACGGCCTCCTGGCATTCTCAGAGTTCCTCGTGATCCGCTCGATGCCACTGCTGTTGCTGAAGCTGTCTCGGCCATTTCTTGCTGGTACGAGGACAAGACCGAATGGGGAGACCATTTGGGGTGGATTTACGGGCCAGTGACAGAAGATGTGGTGACAGGGTACAAAATGCACAACCGAGGATGGCGTTCGGTGTACTGTGTTACCAAGCGTGATGCATTTCGAGGATCAGCGTCCATTAATCTCACTGATCGACTCCACCAAGTACTCCGTTGGGCAACAGGCTCTGTCGAAATTTTCTACTCTCGGAACAATGCCTTCCTTGCCTCATTGCGCCTCAAGTTCCTACAGCGTATTGCCTACATCAATCTCGGTGTCTACCCTTTCACCTCGATCTTTCTCGTCGTGTACTGCTTCCTGCCTGCGCTCTGTCTCTTCACAGGACAGTTCATCGTGGCGAATCTCAGCATCACGTTTTTGATCTACTTGCTAATCATCACCATATGCCTCATTGCTCTGGCCATCCTGGAGGTGAAGTGGTCAGGGATCGAGTTGGAAGAGTGGTGGCGAAACGAGCAGTTTTGGCTCATCTCCGGAACCAGCTCTCACTTGGCTGCTGTTGTGGCAGGGCTTCTAAAAGTGATTGGAGGGATTGAAATTTACTCTACCTCGACTTCCAAGCCAGCTGGAGAGGACAATGATGATATATATGCTGACCTCTACCTTGTGAAGTGGACTTCCCTCATGATCCCTCCAATTGTGATTGGAATGGTGAACCTAATTGCCATTGCCGTCGCGATTTCAAGGGAGATTTATGCTCTGAATCCTGGGTGGGCGAAGCTGATCCGCGGTGTGTTCTTCAGCTTTTGGGTTTTGGCTCACTTGTATCCTTTTGCCAAGGGTTTGATGGGAAGAAGAAGGAAGACGCCTACCATTGTGTTTGTTTGGTCAGGTCTCATTGCCATTACACTTTCCTTGCTCTGGGTCGCCATTAACCCGCCAGCTCCTGGTGCTGCTGGCGTTGCAGGAGCCGGTTTCCAATTTCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000030 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008152 GO:0009846 GO:0009856 GO:0009987 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0019187 GO:0022414 GO:0032501 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0051704 GO:0051753 GO:0070085 GO:0071554 GO:0071669 GO:0097502

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1132

Amino Acids

126.3

Weight (kDa)

6.08

Isoelectric Point (pI)

40.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	133 - 181	5.3e-15	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	368 - 1117	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38190
fragaria_vesca	FvH4_2g17120
malus_domestica	MD05G1144100.v1.1 MD05G1144400.v1.1 MD10G1143700.v1.1
prunus_persica	Prupe.8G182500_v2.0.a1
pyrus_communis	pycom05g13840 pycom05g13860 pycom05g13910 pycom05g13940 pycom10g12370
rosa_chinensis	RchiOBHm_Chr6g0281541
rosa_laevigata	RLG00000012965
rosa_multiflora	Rmu_sc0012649.1_g000003
rosa_roxburghii	Rroxscaffold_7G00187020
rosa_rugosa	Rorug06G0141300 Rorug06G0141400
rosa_samantha	Rh6AG253900 Rh6BG256800 Rh6CG256000 Rh6DG248000
rosa_wichuraiana	Rw6G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1286
Acc16I	TGCGCA	1 cut(s)	321
AccB1I	GGYRCC	1 cut(s)	1808
AccB7I	CCANNNNNTGG	2 cut(s)	235, 3353
AccBSI	CCGCTC	1 cut(s)	2386
AccI	GTMKAC	2 cut(s)	1758, 2724
AccII	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
AccIII	TCCGGA	1 cut(s)	2939
AcoI	YGGCCR	4 cut(s)	91, 1778, 2418, 2865
AcsI	RAATTY	7 cut(s)	280, 656, 2131, 2266, 2342, 2640, 3003
AcuI	CTGAAG	3 cut(s)	1637, 2427, 3190
AcyI	GRCGYC	2 cut(s)	1809, 3272
AfaI	GTAC	7 cut(s)	2114, 2306, 2435, 2510, 2543, 2613, 2756
AflIII	ACRYGT	2 cut(s)	624, 1761
AjiI	CACGTC	1 cut(s)	1135
AleI	CACNNNNGTG	1 cut(s)	1730
Alw26I	GTCTC	7 cut(s)	543, 677, 1222, 2419, 2451, 2783, 3305
AlwNI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
Aor13HI	TCCGGA	1 cut(s)	2939
ApeKI	GCWGC	7 cut(s)	525, 589, 830, 1232, 1265, 2960, 3359
ApoI	RAATTY	7 cut(s)	280, 656, 2131, 2266, 2342, 2640, 3003
ArsI	GACNNNNNNTTYG	2 cut(s)	1322, 1354
AseI	ATTAAT	3 cut(s)	872, 1014, 2586
Asp700I	GAANNNNTTC	1 cut(s)	118
AspLEI	GCGC	9 cut(s)	112, 322, 1811, 1822, 2046, 2048, 2050, 2682, 2773
AspS9I	GGNCC	8 cut(s)	602, 1081, 1288, 1470, 1853, 2066, 2088, 2480
AsuC2I	CCSGG	5 cut(s)	181, 221, 522, 1193, 2086
AsuII	TTCGAA	1 cut(s)	2270
AvaII	GGWCC	5 cut(s)	602, 1288, 1470, 2066, 2088
BalI	TGGCCA	2 cut(s)	93, 2867
BanI	GGYRCC	1 cut(s)	1808
BanII	GRGCYC	1 cut(s)	773
BauI	CACGAG	2 cut(s)	315, 2376
BbsI	GAAGAC	2 cut(s)	159, 3275
BbvCI	CCTCAGC	1 cut(s)	806
BbvI	GCAGC	7 cut(s)	512, 576, 817, 1219, 1252, 2947, 3346
BceAI	ACGGC	6 cut(s)	10, 1166, 1867, 2147, 2366, 3122
BcgI	CGANNNNNNTGC	4 cut(s)	753, 787, 2378, 2412
BciVI	GTATCC	2 cut(s)	1126, 3243
BclI	TGATCA	4 cut(s)	661, 988, 1879, 2326
BcnI	CCSGG	5 cut(s)	181, 221, 522, 1193, 2086
BcoDI	GTCTC	7 cut(s)	543, 677, 1222, 2419, 2451, 2783, 3305
BfaI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
BfmI	CTRYAG	4 cut(s)	475, 847, 2334, 2693
BfoI	RGCGCY	1 cut(s)	1812
BfuI	GTATCC	2 cut(s)	1126, 3243
BglII	AGATCT	1 cut(s)	2230
BisI	GCNGC	8 cut(s)	526, 590, 831, 1233, 1266, 1778, 2961, 3360
BlsI	GCNGC	8 cut(s)	527, 591, 832, 1234, 1267, 1779, 2962, 3361
BmcAI	AGTACT	1 cut(s)	2613
Bme18I	GGWCC	5 cut(s)	602, 1288, 1470, 2066, 2088
BmgBI	CACGTC	1 cut(s)	1135
BmgT120I	GGNCC	8 cut(s)	602, 1081, 1288, 1470, 1853, 2066, 2088, 2480
BpiI	GAAGAC	2 cut(s)	159, 3275
BplI	GAGNNNNNCTC	2 cut(s)	1221, 1253
BpmI	CTGGAG	4 cut(s)	393, 1562, 2894, 3054
Bpu10I	CCTNAGC	1 cut(s)	806
Bpu14I	TTCGAA	1 cut(s)	2270
BpuEI	CTTGAG	2 cut(s)	54, 2669
BpuMI	CCSGG	5 cut(s)	181, 221, 522, 1193, 2086
Bsa29I	ATCGAT	1 cut(s)	1989
BsaBI	GATNNNNATC	2 cut(s)	486, 2229
BsaHI	GRCGYC	2 cut(s)	1809, 3272
BsaI	GGTCTC	3 cut(s)	677, 2451, 3305
BsaWI	WCCGGW	2 cut(s)	293, 2939
BsaXI	ACNNNNNCTCC	2 cut(s)	2867, 2897
Bse118I	RCCGGY	3 cut(s)	462, 1083, 3377
Bse1I	ACTGG	5 cut(s)	514, 1595, 2147, 2294, 2483
Bse3DI	GCAATG	6 cut(s)	715, 1260, 2675, 2856, 3132, 3303
Bse8I	GATNNNNATC	2 cut(s)	486, 2229
BseAI	TCCGGA	1 cut(s)	2939
BseCI	ATCGAT	1 cut(s)	1989
BseJI	GATNNNNATC	2 cut(s)	486, 2229
BseMI	GCAATG	6 cut(s)	715, 1260, 2675, 2856, 3132, 3303
BseMII	CTCAG	5 cut(s)	377, 820, 2292, 2380, 2824
BseNI	ACTGG	5 cut(s)	514, 1595, 2147, 2294, 2483
BsePI	GCGCGC	2 cut(s)	2044, 2046
BseRI	GAGGAG	3 cut(s)	278, 450, 1229
BseXI	GCAGC	7 cut(s)	512, 576, 817, 1219, 1252, 2947, 3346
BseYI	CCCAGC	1 cut(s)	826
Bsh1236I	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
BshNI	GGYRCC	1 cut(s)	1808
BshVI	ATCGAT	1 cut(s)	1989
BslFI	GGGAC	4 cut(s)	197, 706, 1274, 2101
BsmAI	GTCTC	7 cut(s)	543, 677, 1222, 2419, 2451, 2783, 3305
BsmBI	CGTCTC	2 cut(s)	543, 1222
BsmFI	GGGAC	4 cut(s)	197, 706, 1274, 2101
BsmI	GAATGC	3 cut(s)	1531, 1822, 2361
Bso31I	GGTCTC	3 cut(s)	677, 2451, 3305
Bsp119I	TTCGAA	1 cut(s)	2270
Bsp1286I	GDGCHC	1 cut(s)	773
Bsp13I	TCCGGA	1 cut(s)	2939
Bsp19I	CCATGG	1 cut(s)	234
Bsp68I	TCGCGA	3 cut(s)	1343, 1768, 3143
BspCNI	CTCAG	5 cut(s)	376, 819, 2293, 2379, 2823
BspDI	ATCGAT	1 cut(s)	1989
BspEI	TCCGGA	1 cut(s)	2939
BspFNI	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
BspHI	TCATGA	2 cut(s)	1926, 3090
BspMAI	CTGCAG	1 cut(s)	2338
BspT104I	TTCGAA	1 cut(s)	2270
BspT107I	GGYRCC	1 cut(s)	1808
BspTNI	GGTCTC	3 cut(s)	677, 2451, 3305
BsrBI	CCGCTC	1 cut(s)	2386
BsrDI	GCAATG	6 cut(s)	715, 1260, 2675, 2856, 3132, 3303
BsrFI	RCCGGY	3 cut(s)	462, 1083, 3377
BsrI	ACTGG	5 cut(s)	514, 1595, 2147, 2294, 2483
BssAI	RCCGGY	3 cut(s)	462, 1083, 3377
BssHII	GCGCGC	2 cut(s)	2044, 2046
BssNI	GRCGYC	2 cut(s)	1809, 3272
BssSI	CACGAG	2 cut(s)	315, 2376
BssT1I	CCWWGG	4 cut(s)	234, 794, 2308, 3243
Bst2BI	CACGAG	2 cut(s)	315, 2376
Bst6I	CTCTTC	3 cut(s)	632, 2786, 2898
BstACI	GRCGYC	2 cut(s)	1809, 3272
BstBI	TTCGAA	1 cut(s)	2270
BstDEI	CTNAG	7 cut(s)	363, 806, 999, 2136, 2301, 2366, 2810
BstDSI	CCRYGG	2 cut(s)	234, 3193
BstFNI	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
BstH2I	RGCGCY	1 cut(s)	1812
BstHHI	GCGC	9 cut(s)	112, 322, 1811, 1822, 2046, 2048, 2050, 2682, 2773
BstMAI	GTCTC	7 cut(s)	543, 677, 1222, 2419, 2451, 2783, 3305
BstNSI	RCATGY	4 cut(s)	393, 628, 1060, 1765
BstSFI	CTRYAG	4 cut(s)	475, 847, 2334, 2693
BstUI	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
BstV1I	GCAGC	7 cut(s)	512, 576, 817, 1219, 1252, 2947, 3346
BstV2I	GAAGAC	2 cut(s)	159, 3275
BstX2I	RGATCY	1 cut(s)	2230
BstXI	CCANNNNNNTGG	1 cut(s)	1078
BstYI	RGATCY	1 cut(s)	2230
Bsu15I	ATCGAT	1 cut(s)	1989
BsuI	GTATCC	2 cut(s)	1126, 3243
BsuTUI	ATCGAT	1 cut(s)	1989
BtgI	CCRYGG	2 cut(s)	234, 3193
BtrI	CACGTC	1 cut(s)	1135
BtsI	GCAGTG	4 cut(s)	1119, 1152, 2343, 2394
BtuMI	TCGCGA	3 cut(s)	1343, 1768, 3143
CaiI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
CciI	TCATGA	2 cut(s)	1926, 3090
CfoI	GCGC	9 cut(s)	112, 322, 1811, 1822, 2046, 2048, 2050, 2682, 2773
Cfr10I	RCCGGY	3 cut(s)	462, 1083, 3377
Cfr13I	GGNCC	8 cut(s)	602, 1081, 1288, 1470, 1853, 2066, 2088, 2480
Cfr42I	CCGCGG	1 cut(s)	3196
ClaI	ATCGAT	1 cut(s)	1989
CpoI	CGGWCCG	1 cut(s)	1470
CseI	GACGC	2 cut(s)	2568, 3280
Csp6I	GTAC	7 cut(s)	2113, 2305, 2434, 2509, 2542, 2612, 2755
CspCI	CAANNNNNGTGG	2 cut(s)	2383, 2418
CspI	CGGWCCG	1 cut(s)	1470
CviQI	GTAC	7 cut(s)	2113, 2305, 2434, 2509, 2542, 2612, 2755
DdeI	CTNAG	7 cut(s)	363, 806, 999, 2136, 2301, 2366, 2810
DinI	GGCGCC	1 cut(s)	1810
DrdI	GACNNNNNNGTC	1 cut(s)	1286
DseDI	GACNNNNNNGTC	1 cut(s)	1286
EaeI	YGGCCR	4 cut(s)	91, 1778, 2418, 2865
Eam1104I	CTCTTC	3 cut(s)	632, 2786, 2898
EarI	CTCTTC	3 cut(s)	632, 2786, 2898
EciI	GGCGGA	3 cut(s)	1274, 1825, 2297
Eco130I	CCWWGG	4 cut(s)	234, 794, 2308, 3243
Eco24I	GRGCYC	1 cut(s)	773
Eco31I	GGTCTC	3 cut(s)	677, 2451, 3305
Eco47I	GGWCC	5 cut(s)	602, 1288, 1470, 2066, 2088
Eco57I	CTGAAG	3 cut(s)	1637, 2427, 3190
EcoO109I	RGGNCCY	1 cut(s)	1288
EcoT14I	CCWWGG	4 cut(s)	234, 794, 2308, 3243
EcoT22I	ATGCAT	2 cut(s)	395, 2566
EcoT38I	GRGCYC	1 cut(s)	773
EgeI	GGCGCC	1 cut(s)	1810
EheI	GGCGCC	1 cut(s)	1810
ErhI	CCWWGG	4 cut(s)	234, 794, 2308, 3243
Esp3I	CGTCTC	2 cut(s)	543, 1222
FaqI	GGGAC	4 cut(s)	197, 706, 1274, 2101
FauI	CCCGC	1 cut(s)	3351
FauNDI	CATATG	2 cut(s)	1515, 2849
FbaI	TGATCA	4 cut(s)	661, 988, 1879, 2326
FblI	GTMKAC	2 cut(s)	1758, 2724
Fnu4HI	GCNGC	8 cut(s)	526, 590, 831, 1233, 1266, 1778, 2961, 3360
FriOI	GRGCYC	1 cut(s)	773
Fsp4HI	GCNGC	8 cut(s)	526, 590, 831, 1233, 1266, 1778, 2961, 3360
FspAI	RTGCGCAY	1 cut(s)	321
FspBI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	9 cut(s)	111, 321, 1810, 1821, 2045, 2047, 2049, 2681, 2772
GluI	GCNGC	8 cut(s)	526, 590, 831, 1233, 1266, 1778, 2961, 3360
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	4 cut(s)	393, 1562, 2894, 3054
HaeII	RGCGCY	1 cut(s)	1812
HgaI	GACGC	2 cut(s)	2568, 3280
HhaI	GCGC	9 cut(s)	112, 322, 1811, 1822, 2046, 2048, 2050, 2682, 2773
Hin1I	GRCGYC	2 cut(s)	1809, 3272
Hin6I	GCGC	9 cut(s)	110, 320, 1809, 1820, 2044, 2046, 2048, 2680, 2771
HinP1I	GCGC	9 cut(s)	110, 320, 1809, 1820, 2044, 2046, 2048, 2680, 2771
HincII	GTYRAC	2 cut(s)	6, 1354
HindII	GTYRAC	2 cut(s)	6, 1354
Hpy99I	CGWCG	3 cut(s)	173, 2753, 3143
HpyCH4IV	ACGT	4 cut(s)	695, 1134, 1215, 2819
HpyF3I	CTNAG	7 cut(s)	363, 806, 999, 2136, 2301, 2366, 2810
HpySE526I	ACGT	4 cut(s)	695, 1134, 1215, 2819
Hsp92I	GRCGYC	2 cut(s)	1809, 3272
HspAI	GCGC	9 cut(s)	110, 320, 1809, 1820, 2044, 2046, 2048, 2680, 2771
KasI	GGCGCC	1 cut(s)	1808
KflI	GGGWCCC	1 cut(s)	1288
Kpn2I	TCCGGA	1 cut(s)	2939
Ksp22I	TGATCA	4 cut(s)	661, 988, 1879, 2326
KspI	CCGCGG	1 cut(s)	3196
LmnI	GCTCC	8 cut(s)	265, 374, 532, 1138, 1229, 1543, 3355, 3374
Lsp1109I	GCAGC	7 cut(s)	512, 576, 817, 1219, 1252, 2947, 3346
MaeI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
MaeII	ACGT	4 cut(s)	695, 1134, 1215, 2819
MaeIII	GTNAC	8 cut(s)	310, 352, 852, 1334, 1555, 2485, 2500, 2548
MbiI	CCGCTC	1 cut(s)	2386
MfeI	CAATTG	2 cut(s)	593, 3102
MflI	RGATCY	1 cut(s)	2230
MhlI	GDGCHC	1 cut(s)	773
MlsI	TGGCCA	2 cut(s)	93, 2867
MluNI	TGGCCA	2 cut(s)	93, 2867
Mly113I	GGCGCC	1 cut(s)	1809
MlyI	GAGTC	4 cut(s)	307, 349, 988, 2595
MmeI	TCCRAC	2 cut(s)	192, 2880
Mox20I	TGGCCA	2 cut(s)	93, 2867
Mph1103I	ATGCAT	2 cut(s)	395, 2566
MroI	TCCGGA	1 cut(s)	2939
MroXI	GAANNNNTTC	1 cut(s)	118
MscI	TGGCCA	2 cut(s)	93, 2867
MseI	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
MslI	CAYNNNNRTG	3 cut(s)	629, 1623, 1730
Msp20I	TGGCCA	2 cut(s)	93, 2867
MspA1I	CMGCKG	4 cut(s)	830, 2071, 3032, 3195
MunI	CAATTG	2 cut(s)	593, 3102
Mva1269I	GAATGC	3 cut(s)	1531, 1822, 2361
MvnI	CGCG	8 cut(s)	112, 1277, 1343, 1768, 2046, 2048, 3143, 3195
NarI	GGCGCC	1 cut(s)	1809
NciI	CCSGG	5 cut(s)	181, 221, 522, 1193, 2086
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	2 cut(s)	1515, 2849
NmeAIII	GCCGAG	2 cut(s)	1344, 2396
NmuCI	GTSAC	5 cut(s)	310, 352, 1555, 2485, 2500
NruI	TCGCGA	3 cut(s)	1343, 1768, 3143
NsbI	TGCGCA	1 cut(s)	321
NsiI	ATGCAT	2 cut(s)	395, 2566
NspI	RCATGY	4 cut(s)	393, 628, 1060, 1765
NspV	TTCGAA	1 cut(s)	2270
OliI	CACNNNNGTG	1 cut(s)	1730
PaeI	GCATGC	1 cut(s)	393
PagI	TCATGA	2 cut(s)	1926, 3090
PauI	GCGCGC	2 cut(s)	2044, 2046
PciI	ACATGT	2 cut(s)	624, 1761
PcsI	WCGNNNNNNNCGW	2 cut(s)	1449, 1986
PctI	GAATGC	3 cut(s)	1531, 1822, 2361
PdmI	GAANNNNTTC	1 cut(s)	118
PfeI	GAWTC	9 cut(s)	139, 344, 982, 1324, 1454, 1977, 2285, 2806, 3169
PflFI	GACNNNGTC	1 cut(s)	2188
PflMI	CCANNNNNTGG	2 cut(s)	235, 3353
PfoI	TCCNGGA	1 cut(s)	2871
PkrI	GCNGC	8 cut(s)	527, 591, 832, 1234, 1267, 1779, 2962, 3361
PleI	GAGTC	4 cut(s)	307, 349, 988, 2595
PluTI	GGCGCC	1 cut(s)	1812
PpsI	GAGTC	4 cut(s)	307, 349, 988, 2595
PpuMI	RGGWCCY	1 cut(s)	1288
PscI	ACATGT	2 cut(s)	624, 1761
PshBI	ATTAAT	3 cut(s)	872, 1014, 2586
Psp5II	RGGWCCY	1 cut(s)	1288
PspFI	CCCAGC	1 cut(s)	826
PspPI	GGNCC	8 cut(s)	602, 1081, 1288, 1470, 1853, 2066, 2088, 2480
PspPPI	RGGWCCY	1 cut(s)	1288
PsrI	GAACNNNNNNTAC	2 cut(s)	1409, 1441
PstI	CTGCAG	1 cut(s)	2338
PstNI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
PsuI	RGATCY	1 cut(s)	2230
PsyI	GACNNNGTC	1 cut(s)	2188
PteI	GCGCGC	2 cut(s)	2044, 2046
PvuII	CAGCTG	2 cut(s)	830, 3032
RruI	TCGCGA	3 cut(s)	1343, 1768, 3143
RsaI	GTAC	7 cut(s)	2114, 2306, 2435, 2510, 2543, 2613, 2756
RsaNI	GTAC	7 cut(s)	2113, 2305, 2434, 2509, 2542, 2612, 2755
RseI	CAYNNNNRTG	3 cut(s)	629, 1623, 1730
Rsr2I	CGGWCCG	1 cut(s)	1470
RsrII	CGGWCCG	1 cut(s)	1470
SacII	CCGCGG	1 cut(s)	3196
SaqAI	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
SatI	GCNGC	8 cut(s)	526, 590, 831, 1233, 1266, 1778, 2961, 3360
Sau96I	GGNCC	8 cut(s)	602, 1081, 1288, 1470, 1853, 2066, 2088, 2480
ScaI	AGTACT	1 cut(s)	2613
SchI	GAGTC	4 cut(s)	307, 349, 988, 2595
SduI	GDGCHC	1 cut(s)	773
SfcI	CTRYAG	4 cut(s)	475, 847, 2334, 2693
SfoI	GGCGCC	1 cut(s)	1810
Sfr303I	CCGCGG	1 cut(s)	3196
SfuI	TTCGAA	1 cut(s)	2270
SgrBI	CCGCGG	1 cut(s)	3196
SinI	GGWCC	5 cut(s)	602, 1288, 1470, 2066, 2088
SmiMI	CAYNNNNRTG	3 cut(s)	629, 1623, 1730
SmlI	CTYRAG	2 cut(s)	69, 2684
SmoI	CTYRAG	2 cut(s)	69, 2684
SphI	GCATGC	1 cut(s)	393
SspDI	GGCGCC	1 cut(s)	1808
SspMI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
StyI	CCWWGG	4 cut(s)	234, 794, 2308, 3243
TaiI	ACGT	4 cut(s)	698, 1137, 1218, 2822
TaqII	GACCGA	2 cut(s)	1040, 2462
TatI	WGTACW	3 cut(s)	2541, 2611, 2754
TauI	GCSGC	1 cut(s)	1780
TfiI	GAWTC	9 cut(s)	139, 344, 982, 1324, 1454, 1977, 2285, 2806, 3169
Tru1I	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
Tru9I	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
TseFI	GTSAC	5 cut(s)	310, 352, 1555, 2485, 2500
TseI	GCWGC	7 cut(s)	525, 589, 830, 1232, 1265, 2960, 3359
Tsp45I	GTSAC	5 cut(s)	310, 352, 1555, 2485, 2500
TspDTI	ATGAA	8 cut(s)	194, 206, 437, 650, 1514, 1829, 1915, 2785
TspGWI	ACGGA	5 cut(s)	235, 624, 1898, 2606, 3381
Tth111I	GACNNNGTC	1 cut(s)	2188
Van91I	CCANNNNNTGG	2 cut(s)	235, 3353
VpaK11BI	GGWCC	5 cut(s)	602, 1288, 1470, 2066, 2088
VspI	ATTAAT	3 cut(s)	872, 1014, 2586
XapI	RAATTY	7 cut(s)	280, 656, 2131, 2266, 2342, 2640, 3003
XceI	RCATGY	4 cut(s)	393, 628, 1060, 1765
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1819, 2953, 3250
XmiI	GTMKAC	2 cut(s)	1758, 2724
XmnI	GAANNNNTTC	1 cut(s)	118
XspI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
ZrmI	AGTACT	1 cut(s)	2613
Zsp2I	ATGCAT	2 cut(s)	395, 2566

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.