Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008941

Physical Location & Seq

Genomic Coordinates Forward (+)

23075540 .. 23080296

Gene Structure 4757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1143700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3366 bp

ATGGCAGCGTCCCTGAATAGAGAGCCATCGAAAAAGGCAATAAAAAGCCCTGGAGGTTCTGGTAGCTCTCAAGGCAAAGCTAATTCGAGTGGCCAAACTGTGAAGTTTGCGCGAAGAACTTCAAGTGGACGGTATGTGAGTCTGTCTAGAGAAGACCTTGATATGTCCGGAGAATTATCCGGGGACTATATGGACTACACAGTACAGATTCCTGCCACCCCAGATAACCAGCCCATGGACTCATCCGGGGCTGTGAAGGCGGAGGAGCAATATGTTTCTAACTCTTTATTTACCGGAGGGTTCAATAGTACGACGCGTGCGCATCTCATGGATAAGGTGATTGATTCGGAGGTGACTCATCCTCAGATGGCTGGAGCCAAAGGTTCTGCATGTATGATGCCTGCTTGTGATGGTAAGGTGATGAAGGATGAGAGAGGAGTTGATATAACCCCTTGTGATTGCAGGTTCAAAATCTGTAGAGATTGCTATTTGGATGCACAGAAGAACACTGGCCTTTGTCCGGGTTGCAAGGAGCAATACAGAGTAGGAGACGAATTTGGTGATCCATCGGGTTACAACAATGGAACGCTGCAATTGCCTGGTGCCGATGGAAAAAGGGATAACATGTCTGCGATGAAGAGGAACCAAACCGGAGAACTTGATCACAATAGGTGGTTGTTTGAGAACAAGGGGACTTATGGTGATGGCAATGCTTTCGATCCCCAAGATGACGGGTATGGTGATGGCGGTGGTGATGGCTTCGGAGGGGGCTTGCTGGATGATGACAAGCCTTGGAAACCCCTCGGCAAGATATTGCCAATTGCTACTGCCATTATCACCCCCTACAGGATACTGATCTTCGTTCGGTTAATCGTGCTAAGTTTGTTCCTGCATTGGAGAGTAGTCAATCCAAACAATGATGCAAGATGGCTGTGGCTCATGTCGATTATCTGCGAAATATGGTTCGCCTTCTCTTGGATTCTTGATCAGACTCCTAAATTTTTCCCCATTAATCGTCAGACCTATCTTGAAGTCCTCCACGACAAGTTTGACAGGCCATCACCATCCAATCCAACGGGCCGGTCTGACCTCCCTGGCACTGACTTCTTTGTATCTACTGCTGATCCTGACAAAGAGCCACCTCTCACCACTGCCAACACCATCCTTTCAATCCTAGCCATTGATTACCCAGTCGAAAAGCTAGCATGCTACGTCTCTGATGATGGAGGTGCCCTCCTCACCTTTGAGGCAATGGCGGAAGCTGCTAGTTTCGCAGACTTGTGGGTTCCCTTCTGCCGGAAACACAACATTGAGCCGAGGAATCCTGACAGTTACTTTGCGTTGAAAGTTGACCCGACAAAGAACAAGAGTAGGCTGGACTTTGTGAAGGATAGGAGGAAGATCAAGAGGGAGTATGATGAGTTCAAGGTGAGGATCAACAGTCTTCCGGTTTCGATCAGGAGGCGGTCTGATGCTTTCCATGCCAGGGAGGAAATGCAGCAGTTGAAGCATATGAGGGAGAATGGAATTGACCCTTTGGAGCAAGTCAAAGTCCCCAAGGCTACCTGGATGGCTGATGGCACACATTGGCCTGGTGCTTGGTCTGTTCCTTCCAGTGATCATGCCAAAGGTGACCATTCAGGAATTCTTCAGGTGATGCTGAAGCCTCCTAGTCCTGACCCCCTAATGGGAAGTGCCGATGATGACAAGCTCATAGATTTCACAGATGTGGATATACGCCTCCCAATGTTTGTCTACATGTCGCGAGAAAAGCGGCCTGGCTATGATCACAACAAGAAAGCCGGCGCCATGAATGCGCTGGTGAGAGCATCCGCCATCTTGTCAAACGGCCCTTTCATTCTCAACCTTGACTGTGATCACTACATCTACAACTGCAAAGCTGTCCGTGAAGGGATGTGCTTCATGATGGACAAAGGCGGTGAAAACATCTGCTACATTCAGTTTCCTCAGAGATTCGAAGGCATTGATCCCTCCGATCGCTATGCCAATCACAACACCGTGTTTTTCGATGGCAATATGCGTGCACTTGATGGTTTGCAGGGTCCGATGTATGTGGGAACCGGGACCATGTTCCGGCGTTTTGCCTTGTACGGTTTTGATCCACCAAATCCTGACAAGTTGCCGGTGAAGAAGGATGATGAGGCACAAGGAGAGCCTTTGACAACGTCGAATACACAACCTTTGACAGCCAGTGACTTTGACCCGGATCTTGACACCAATCTACTTCCCAAGCGTTTTGGAAATTCAACAATGCTGGCGGAATCCATACCCGTTGCTGAGTACCAAGGCCGCCCCGTAGCTGATCATCCTTCAGTGAAATTCGGACGGCCTCCTGGCGCTCTCAGAGTTCCTCGTGATCCGCTCAATGCCACTACTGTTGCTGAAGCTGTCTCTGCCATTTCTTGCTGGTACGAGGACAAGACCGAATGGGGAGACCGTGTGGGGTGGATTTACGGGTCAGTGACAGAAGATGTGGTGACTGGGTACAGAATGCACAATCGCGGATGGCGCTCGGTGTACTGCGTTACCAAGCGTGACGCATTTCGCGGTTCAGCTCCCATTAATCTTACTGATCGACTCCACCAAGTGCTCCGTTGGGCAACTGGCTCAGTCGAGATTTTTTTCTCCCGGAACAACGCCTTCCTTGCCTCAATGCGCCTCAAGTTACTACAACGTCTTGCCTACATCAATGTCGGCATCTACCCTTTCACCTCGATCTTTCTCATCGTGTACTGCTTCCTCCCTGCGCTTTCGCTCTTCAGTGGACAGTTCATTGTGGCGAATCTCAACATCACATTTTTGGTCTACTTGCTAACCATTACTATATGCCTCATATCTTTGGCTATCCTGGAGGTGAAGTGGTCGGGGATCGCGTTGGAAGAGTGGTGGCGAAATGAGCAGTTTTGGCTGATTTCCGGAACCAGCGCTCACTTGGCTGCTGTGGTGCAAGGCCTTCTAAAGGTGATTGCAGGGATTGAAATTTCCTTTACCTTGACGGCCAAGTCAGTTGGAGAGGACAACGAAGATATATATGCTGACCTCTACCTTGTGAAGTGGACTTCCCTCATGATCCCTCCAATTGTGATTGCAATGGTGAACATAATCGCAATTGCAGTCGCGTTTTCAAGGGAGGTTTACGCTCTGAATCCTCAGTGGGCGAGGTTTATTGGCGGTGCCTTCTTCAGCTTTTGGGTTTTGGCTCACTTGTATCCTTTTGCCAAGGGTTTGATGGGAAGAAGAAGGAAGACCCCTACCATTGTGTTTGTTTGGTCAGGTCTCATTGCAATTACACTTTCCTTGCTCTGGGTCGCCATTAACCCGCCCGCCGTTGTTGTCGTTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000030 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008152 GO:0009846 GO:0009856 GO:0009987 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0019187 GO:0022414 GO:0032501 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0051704 GO:0051753 GO:0070085 GO:0071554 GO:0071669 GO:0097502

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1122

Amino Acids

125.11

Weight (kDa)

6.22

Isoelectric Point (pI)

39.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	133 - 181	3.2e-15	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	368 - 1115	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38190
fragaria_vesca	FvH4_2g17120
malus_domestica	MD05G1144100.v1.1 MD05G1144400.v1.1 MD10G1143700.v1.1
prunus_persica	Prupe.8G182500_v2.0.a1
pyrus_communis	pycom05g13840 pycom05g13860 pycom05g13910 pycom05g13940 pycom10g12370
rosa_chinensis	RchiOBHm_Chr6g0281541
rosa_laevigata	RLG00000012965
rosa_multiflora	Rmu_sc0012649.1_g000003
rosa_roxburghii	Rroxscaffold_7G00187020
rosa_rugosa	Rorug06G0141300 Rorug06G0141400
rosa_samantha	Rh6AG253900 Rh6BG256800 Rh6CG256000 Rh6DG248000
rosa_wichuraiana	Rw6G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	3022
Acc16I	TGCGCA	1 cut(s)	321
Acc36I	ACCTGC	1 cut(s)	453
AccB1I	GGYRCC	4 cut(s)	602, 1229, 1805, 3194
AccB7I	CCANNNNNTGG	1 cut(s)	235
AccBSI	CCGCTC	1 cut(s)	2383
AccI	GTMKAC	2 cut(s)	1755, 2826
AccII	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
AccIII	TCCGGA	2 cut(s)	167, 2936
AcoI	YGGCCR	2 cut(s)	91, 3018
AcsI	RAATTY	6 cut(s)	554, 998, 1644, 2263, 2339, 3000
AcuI	CTGAAG	6 cut(s)	1634, 1682, 2316, 2424, 2764, 3187
AcyI	GRCGYC	1 cut(s)	1806
AdeI	CACNNNGTG	1 cut(s)	2608
AfaI	GTAC	8 cut(s)	204, 310, 2111, 2303, 2432, 2507, 2540, 2753
AfeI	AGCGCT	1 cut(s)	2947
AflIII	ACRYGT	3 cut(s)	314, 624, 1758
AjnI	CCWGG	9 cut(s)	49, 598, 1093, 1484, 1565, 1591, 1777, 2353, 2868
AleI	CACNNNNGTG	1 cut(s)	1727
Alw21I	GWGCWC	2 cut(s)	2047, 2613
Alw26I	GTCTC	5 cut(s)	543, 1219, 2416, 2448, 3302
Alw44I	GTGCAC	1 cut(s)	2043
Aor13HI	TCCGGA	2 cut(s)	167, 2936
Aor51HI	AGCGCT	1 cut(s)	2947
ApaLI	GTGCAC	1 cut(s)	2043
ApeKI	GCWGC	5 cut(s)	5, 589, 1262, 1498, 2957
ApoI	RAATTY	6 cut(s)	554, 998, 1644, 2263, 2339, 3000
ArsI	GACNNNNNNTTYG	2 cut(s)	1319, 1351
AseI	ATTAAT	2 cut(s)	1011, 2583
Asp700I	GAANNNNTTC	1 cut(s)	118
AspLEI	GCGC	9 cut(s)	112, 322, 1808, 1819, 2360, 2532, 2679, 2770, 2948
AspS9I	GGNCC	4 cut(s)	1078, 1850, 2063, 2085
AsuC2I	CCSGG	6 cut(s)	181, 247, 522, 2083, 2225, 2650
AsuII	TTCGAA	1 cut(s)	1977
AsuNHI	GCTAGC	1 cut(s)	1201
AvaII	GGWCC	2 cut(s)	2063, 2085
BaeGI	GKGCMC	2 cut(s)	1234, 2047
BalI	TGGCCA	1 cut(s)	93
BanI	GGYRCC	4 cut(s)	602, 1229, 1805, 3194
BauI	CACGAG	1 cut(s)	2373
BbsI	GAAGAC	3 cut(s)	159, 1436, 3272
Bbv12I	GWGCWC	2 cut(s)	2047, 2613
BbvI	GCAGC	5 cut(s)	17, 576, 1249, 1510, 2944
BceAI	ACGGC	4 cut(s)	1864, 2363, 3033, 3332
BcgI	CGANNNNNNTGC	4 cut(s)	697, 731, 1985, 2019
BciT130I	CCWGG	9 cut(s)	51, 600, 1095, 1486, 1567, 1593, 1779, 2355, 2870
BciVI	GTATCC	2 cut(s)	843, 3240
BclI	TGATCA	6 cut(s)	661, 985, 1618, 1786, 1876, 2323
BcnI	CCSGG	6 cut(s)	181, 247, 522, 2083, 2225, 2650
BcoDI	GTCTC	5 cut(s)	543, 1219, 2416, 2448, 3302
BfaI	CTAG	5 cut(s)	147, 1175, 1202, 1266, 1671
BfmI	CTRYAG	2 cut(s)	475, 844
BfoI	RGCGCY	4 cut(s)	1809, 2361, 2533, 2949
BfuAI	ACCTGC	1 cut(s)	453
BfuI	GTATCC	2 cut(s)	843, 3240
BisI	GCNGC	7 cut(s)	6, 590, 1263, 1499, 1775, 2311, 2958
BlsI	GCNGC	7 cut(s)	7, 591, 1264, 1500, 1776, 2312, 2959
Bme18I	GGWCC	2 cut(s)	2063, 2085
BmgT120I	GGNCC	4 cut(s)	1078, 1850, 2063, 2085
BmrI	ACTGGG	2 cut(s)	1184, 2511
BmsI	GCATC	8 cut(s)	331, 387, 484, 910, 1462, 1647, 1838, 2727
BmtI	GCTAGC	1 cut(s)	1205
BmuI	ACTGGG	2 cut(s)	1184, 2511
BpiI	GAAGAC	3 cut(s)	159, 1436, 3272
BplI	GAGNNNNNCTC	2 cut(s)	1218, 1250
BpmI	CTGGAG	3 cut(s)	72, 393, 2891
Bpu14I	TTCGAA	1 cut(s)	1977
BpuEI	CTTGAG	2 cut(s)	54, 2666
BpuMI	CCSGG	6 cut(s)	181, 247, 522, 2083, 2225, 2650
BsaBI	GATNNNNATC	1 cut(s)	854
BsaHI	GRCGYC	1 cut(s)	1806
BsaI	GGTCTC	2 cut(s)	2448, 3302
BsaWI	WCCGGW	5 cut(s)	167, 293, 650, 1447, 2936
BsaXI	ACNNNNNCTCC	4 cut(s)	2445, 2475, 2864, 2894
Bse118I	RCCGGY	3 cut(s)	1080, 1802, 2143
Bse1I	ACTGG	6 cut(s)	514, 1190, 1614, 2211, 2506, 2629
Bse3DI	GCAATG	4 cut(s)	715, 1257, 3117, 3300
Bse8I	GATNNNNATC	1 cut(s)	854
BseAI	TCCGGA	2 cut(s)	167, 2936
BseBI	CCWGG	9 cut(s)	51, 600, 1095, 1486, 1567, 1593, 1779, 2355, 2870
BseJI	GATNNNNATC	1 cut(s)	854
BseMI	GCAATG	4 cut(s)	715, 1257, 3117, 3300
BseMII	CTCAG	6 cut(s)	377, 1982, 2289, 2377, 2643, 3185
BseNI	ACTGG	6 cut(s)	514, 1190, 1614, 2211, 2506, 2629
BseRI	GAGGAG	3 cut(s)	278, 450, 1226
BseSI	GKGCMC	2 cut(s)	1234, 2047
BseXI	GCAGC	5 cut(s)	17, 576, 1249, 1510, 2944
Bsh1236I	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
Bsh1285I	CGRYCG	1 cut(s)	1999
BshNI	GGYRCC	4 cut(s)	602, 1229, 1805, 3194
BsiEI	CGRYCG	1 cut(s)	1999
BsiHKAI	GWGCWC	2 cut(s)	2047, 2613
BslFI	GGGAC	4 cut(s)	197, 706, 1538, 2098
BsmAI	GTCTC	5 cut(s)	543, 1219, 2416, 2448, 3302
BsmBI	CGTCTC	2 cut(s)	543, 1219
BsmFI	GGGAC	4 cut(s)	197, 706, 1538, 2098
BsmI	GAATGC	2 cut(s)	1819, 2517
Bso31I	GGTCTC	2 cut(s)	2448, 3302
Bsp119I	TTCGAA	1 cut(s)	1977
Bsp1286I	GDGCHC	3 cut(s)	1234, 2047, 2613
Bsp13I	TCCGGA	2 cut(s)	167, 2936
Bsp19I	CCATGG	1 cut(s)	234
Bsp68I	TCGCGA	1 cut(s)	1765
BspCNI	CTCAG	6 cut(s)	376, 1981, 2290, 2376, 2642, 3184
BspEI	TCCGGA	2 cut(s)	167, 2936
BspFNI	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
BspHI	TCATGA	2 cut(s)	1923, 3087
BspMI	ACCTGC	1 cut(s)	453
BspOI	GCTAGC	1 cut(s)	1205
BspQI	GCTCTTC	1 cut(s)	2783
BspT104I	TTCGAA	1 cut(s)	1977
BspT107I	GGYRCC	4 cut(s)	602, 1229, 1805, 3194
BspTNI	GGTCTC	2 cut(s)	2448, 3302
BsrBI	CCGCTC	1 cut(s)	2383
BsrDI	GCAATG	4 cut(s)	715, 1257, 3117, 3300
BsrFI	RCCGGY	3 cut(s)	1080, 1802, 2143
BsrI	ACTGG	6 cut(s)	514, 1190, 1614, 2211, 2506, 2629
BssAI	RCCGGY	3 cut(s)	1080, 1802, 2143
BssNI	GRCGYC	1 cut(s)	1806
BssSI	CACGAG	1 cut(s)	2373
BssT1I	CCWWGG	5 cut(s)	234, 791, 1557, 2305, 3240
Bst2BI	CACGAG	1 cut(s)	2373
Bst2UI	CCWGG	9 cut(s)	51, 600, 1095, 1486, 1567, 1593, 1779, 2355, 2870
Bst6I	CTCTTC	3 cut(s)	632, 2783, 2895
BstACI	GRCGYC	1 cut(s)	1806
BstBI	TTCGAA	1 cut(s)	1977
BstC8I	GCNNGC	9 cut(s)	318, 402, 773, 1203, 1207, 1804, 2043, 2277, 3345
BstDEI	CTNAG	7 cut(s)	363, 878, 1968, 2298, 2363, 2629, 3171
BstDSI	CCRYGG	1 cut(s)	234
BstEII	GGTNACC	1 cut(s)	1631
BstENI	CCTNNNNNAGG	1 cut(s)	2978
BstFNI	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
BstH2I	RGCGCY	4 cut(s)	1809, 2361, 2533, 2949
BstHHI	GCGC	9 cut(s)	112, 322, 1808, 1819, 2360, 2532, 2679, 2770, 2948
BstMAI	GTCTC	5 cut(s)	543, 1219, 2416, 2448, 3302
BstMCI	CGRYCG	1 cut(s)	1999
BstNI	CCWGG	9 cut(s)	51, 600, 1095, 1486, 1567, 1593, 1779, 2355, 2870
BstNSI	RCATGY	4 cut(s)	393, 628, 1209, 1762
BstPI	GGTNACC	1 cut(s)	1631
BstSFI	CTRYAG	2 cut(s)	475, 844
BstSLI	GKGCMC	2 cut(s)	1234, 2047
BstUI	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
BstV1I	GCAGC	5 cut(s)	17, 576, 1249, 1510, 2944
BstV2I	GAAGAC	3 cut(s)	159, 1436, 3272
BstX2I	RGATCY	1 cut(s)	2227
BstYI	RGATCY	1 cut(s)	2227
BsuI	GTATCC	2 cut(s)	843, 3240
BtgI	CCRYGG	1 cut(s)	234
BtgZI	GCGATG	1 cut(s)	647
BtsI	GCAGTG	1 cut(s)	1149
BtsIMutI	CAGTG	9 cut(s)	507, 1098, 1149, 1621, 2218, 2340, 2487, 2788, 3179
BtuMI	TCGCGA	1 cut(s)	1765
BveI	ACCTGC	1 cut(s)	453
Cac8I	GCNNGC	9 cut(s)	318, 402, 773, 1203, 1207, 1804, 2043, 2277, 3345
CciI	TCATGA	2 cut(s)	1923, 3087
CfoI	GCGC	9 cut(s)	112, 322, 1808, 1819, 2360, 2532, 2679, 2770, 2948
Cfr10I	RCCGGY	3 cut(s)	1080, 1802, 2143
Cfr13I	GGNCC	4 cut(s)	1078, 1850, 2063, 2085
CseI	GACGC	2 cut(s)	322, 2567
Csp6I	GTAC	8 cut(s)	203, 309, 2110, 2302, 2431, 2506, 2539, 2752
CspCI	CAANNNNNGTGG	2 cut(s)	2380, 2415
CviQI	GTAC	8 cut(s)	203, 309, 2110, 2302, 2431, 2506, 2539, 2752
DdeI	CTNAG	7 cut(s)	363, 878, 1968, 2298, 2363, 2629, 3171
DinI	GGCGCC	1 cut(s)	1807
DraIII	CACNNNGTG	1 cut(s)	2608
DrdI	GACNNNNNNGTC	1 cut(s)	3022
DseDI	GACNNNNNNGTC	1 cut(s)	3022
EaeI	YGGCCR	2 cut(s)	91, 3018
Eam1104I	CTCTTC	3 cut(s)	632, 2783, 2895
EarI	CTCTTC	3 cut(s)	632, 2783, 2895
EciI	GGCGGA	4 cut(s)	275, 1271, 1822, 2294
Eco130I	CCWWGG	5 cut(s)	234, 791, 1557, 2305, 3240
Eco147I	AGGCCT	1 cut(s)	2973
Eco31I	GGTCTC	2 cut(s)	2448, 3302
Eco47I	GGWCC	2 cut(s)	2063, 2085
Eco47III	AGCGCT	1 cut(s)	2947
Eco57I	CTGAAG	6 cut(s)	1634, 1682, 2316, 2424, 2764, 3187
Eco91I	GGTNACC	1 cut(s)	1631
EcoNI	CCTNNNNNAGG	1 cut(s)	2978
EcoO65I	GGTNACC	1 cut(s)	1631
EcoRI	GAATTC	1 cut(s)	1644
EcoRII	CCWGG	9 cut(s)	49, 598, 1093, 1484, 1565, 1591, 1777, 2353, 2868
EcoT14I	CCWWGG	5 cut(s)	234, 791, 1557, 2305, 3240
EgeI	GGCGCC	1 cut(s)	1807
EheI	GGCGCC	1 cut(s)	1807
ErhI	CCWWGG	5 cut(s)	234, 791, 1557, 2305, 3240
Esp3I	CGTCTC	2 cut(s)	543, 1219
FaqI	GGGAC	4 cut(s)	197, 706, 1538, 2098
FauI	CCCGC	2 cut(s)	3348, 3352
FauNDI	CATATG	1 cut(s)	1512
FbaI	TGATCA	6 cut(s)	661, 985, 1618, 1786, 1876, 2323
FblI	GTMKAC	2 cut(s)	1755, 2826
Fnu4HI	GCNGC	7 cut(s)	6, 590, 1263, 1499, 1775, 2311, 2958
Fsp4HI	GCNGC	7 cut(s)	6, 590, 1263, 1499, 1775, 2311, 2958
FspAI	RTGCGCAY	1 cut(s)	321
FspBI	CTAG	5 cut(s)	147, 1175, 1202, 1266, 1671
FspI	TGCGCA	1 cut(s)	321
GlaI	GCGC	9 cut(s)	111, 321, 1807, 1818, 2359, 2531, 2678, 2769, 2947
GluI	GCNGC	7 cut(s)	6, 590, 1263, 1499, 1775, 2311, 2958
GsuI	CTGGAG	3 cut(s)	72, 393, 2891
HaeII	RGCGCY	4 cut(s)	1809, 2361, 2533, 2949
HgaI	GACGC	2 cut(s)	322, 2567
HhaI	GCGC	9 cut(s)	112, 322, 1808, 1819, 2360, 2532, 2679, 2770, 2948
Hin1I	GRCGYC	1 cut(s)	1806
Hin6I	GCGC	9 cut(s)	110, 320, 1806, 1817, 2358, 2530, 2677, 2768, 2946
HinP1I	GCGC	9 cut(s)	110, 320, 1806, 1817, 2358, 2530, 2677, 2768, 2946
HincII	GTYRAC	1 cut(s)	1351
HindII	GTYRAC	1 cut(s)	1351
Hpy99I	CGWCG	2 cut(s)	316, 2191
HpyCH4IV	ACGT	3 cut(s)	1212, 2186, 2695
HpyF3I	CTNAG	7 cut(s)	363, 878, 1968, 2298, 2363, 2629, 3171
HpySE526I	ACGT	3 cut(s)	1212, 2186, 2695
Hsp92I	GRCGYC	1 cut(s)	1806
HspAI	GCGC	9 cut(s)	110, 320, 1806, 1817, 2358, 2530, 2677, 2768, 2946
KasI	GGCGCC	1 cut(s)	1805
Kpn2I	TCCGGA	2 cut(s)	167, 2936
KroI	GCCGGC	1 cut(s)	1802
KroNI	GCCGGC	1 cut(s)	1804
Ksp22I	TGATCA	6 cut(s)	661, 985, 1618, 1786, 1876, 2323
LguI	GCTCTTC	1 cut(s)	2783
LmnI	GCTCC	6 cut(s)	265, 374, 532, 1540, 2581, 2616
Lsp1109I	GCAGC	5 cut(s)	17, 576, 1249, 1510, 2944
LweI	GCATC	8 cut(s)	331, 387, 484, 910, 1462, 1647, 1838, 2727
MaeI	CTAG	5 cut(s)	147, 1175, 1202, 1266, 1671
MaeII	ACGT	3 cut(s)	1212, 2186, 2695
MbiI	CCGCTC	1 cut(s)	2383
MfeI	CAATTG	4 cut(s)	593, 819, 3099, 3129
MflI	RGATCY	1 cut(s)	2227
MhlI	GDGCHC	3 cut(s)	1234, 2047, 2613
MlsI	TGGCCA	1 cut(s)	93
MluI	ACGCGT	1 cut(s)	314
MluNI	TGGCCA	1 cut(s)	93
Mly113I	GGCGCC	1 cut(s)	1806
MlyI	GAGTC	5 cut(s)	148, 233, 349, 985, 2592
MmeI	TCCRAC	3 cut(s)	1097, 2877, 3010
Mox20I	TGGCCA	1 cut(s)	93
MroI	TCCGGA	2 cut(s)	167, 2936
MroNI	GCCGGC	1 cut(s)	1802
MroXI	GAANNNNTTC	1 cut(s)	118
MscI	TGGCCA	1 cut(s)	93
MseI	TTAA	5 cut(s)	869, 1011, 2583, 3336, 3364
MslI	CAYNNNNRTG	1 cut(s)	1727
Msp20I	TGGCCA	1 cut(s)	93
MunI	CAATTG	4 cut(s)	593, 819, 3099, 3129
Mva1269I	GAATGC	2 cut(s)	1819, 2517
MvaI	CCWGG	9 cut(s)	51, 600, 1095, 1486, 1567, 1593, 1779, 2355, 2870
MvnI	CGCG	7 cut(s)	112, 316, 1765, 2523, 2568, 2894, 3140
NaeI	GCCGGC	1 cut(s)	1804
NarI	GGCGCC	1 cut(s)	1806
NciI	CCSGG	6 cut(s)	181, 247, 522, 2083, 2225, 2650
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	1512
NgoMIV	GCCGGC	1 cut(s)	1802
NheI	GCTAGC	1 cut(s)	1201
NmeAIII	GCCGAG	2 cut(s)	783, 1341
NmuCI	GTSAC	6 cut(s)	352, 1631, 2213, 2482, 2497, 2555
NruI	TCGCGA	1 cut(s)	1765
NsbI	TGCGCA	1 cut(s)	321
NspI	RCATGY	4 cut(s)	393, 628, 1209, 1762
NspV	TTCGAA	1 cut(s)	1977
OliI	CACNNNNGTG	1 cut(s)	1727
PaeI	GCATGC	1 cut(s)	1209
PagI	TCATGA	2 cut(s)	1923, 3087
PceI	AGGCCT	1 cut(s)	2973
PciI	ACATGT	2 cut(s)	624, 1758
PciSI	GCTCTTC	1 cut(s)	2783
PcsI	WCGNNNNNNNCGW	1 cut(s)	2891
PctI	GAATGC	2 cut(s)	1819, 2517
PdiI	GCCGGC	1 cut(s)	1804
PdmI	GAANNNNTTC	1 cut(s)	118
PfeI	GAWTC	8 cut(s)	208, 344, 979, 1321, 1974, 2282, 2803, 3166
PflFI	GACNNNGTC	1 cut(s)	2185
PflMI	CCANNNNNTGG	1 cut(s)	235
PfoI	TCCNGGA	2 cut(s)	2648, 2868
PkrI	GCNGC	7 cut(s)	7, 591, 1264, 1500, 1776, 2312, 2959
Ple19I	CGATCG	1 cut(s)	1999
PleI	GAGTC	5 cut(s)	147, 233, 349, 985, 2592
PluTI	GGCGCC	1 cut(s)	1809
PpsI	GAGTC	5 cut(s)	147, 233, 349, 985, 2592
PscI	ACATGT	2 cut(s)	624, 1758
PshBI	ATTAAT	2 cut(s)	1011, 2583
Psp6I	CCWGG	9 cut(s)	49, 598, 1093, 1484, 1565, 1591, 1777, 2353, 2868
PspEI	GGTNACC	1 cut(s)	1631
PspGI	CCWGG	9 cut(s)	49, 598, 1093, 1484, 1565, 1591, 1777, 2353, 2868
PspPI	GGNCC	4 cut(s)	1078, 1850, 2063, 2085
PsrI	GAACNNNNNNTAC	2 cut(s)	1406, 1438
PsuI	RGATCY	1 cut(s)	2227
PsyI	GACNNNGTC	1 cut(s)	2185
PvuI	CGATCG	1 cut(s)	1999
RruI	TCGCGA	1 cut(s)	1765
RsaI	GTAC	8 cut(s)	204, 310, 2111, 2303, 2432, 2507, 2540, 2753
RsaNI	GTAC	8 cut(s)	203, 309, 2110, 2302, 2431, 2506, 2539, 2752
RseI	CAYNNNNRTG	1 cut(s)	1727
SapI	GCTCTTC	1 cut(s)	2783
SaqAI	TTAA	5 cut(s)	869, 1011, 2583, 3336, 3364
SatI	GCNGC	7 cut(s)	6, 590, 1263, 1499, 1775, 2311, 2958
Sau96I	GGNCC	4 cut(s)	1078, 1850, 2063, 2085
SchI	GAGTC	5 cut(s)	148, 233, 349, 985, 2592
SduI	GDGCHC	3 cut(s)	1234, 2047, 2613
SfaNI	GCATC	8 cut(s)	331, 387, 484, 910, 1462, 1647, 1838, 2727
SfcI	CTRYAG	2 cut(s)	475, 844
SfoI	GGCGCC	1 cut(s)	1807
SfuI	TTCGAA	1 cut(s)	1977
SinI	GGWCC	2 cut(s)	2063, 2085
SmiMI	CAYNNNNRTG	1 cut(s)	1727
SmlI	CTYRAG	2 cut(s)	69, 2681
SmoI	CTYRAG	2 cut(s)	69, 2681
SphI	GCATGC	1 cut(s)	1209
SseBI	AGGCCT	1 cut(s)	2973
SspDI	GGCGCC	1 cut(s)	1805
SspMI	CTAG	5 cut(s)	147, 1175, 1202, 1266, 1671
StuI	AGGCCT	1 cut(s)	2973
StyI	CCWWGG	5 cut(s)	234, 791, 1557, 2305, 3240
TaiI	ACGT	3 cut(s)	1215, 2189, 2698
TaqII	GACCGA	1 cut(s)	2459
TatI	WGTACW	3 cut(s)	202, 2538, 2751
TauI	GCSGC	2 cut(s)	1777, 2313
TfiI	GAWTC	8 cut(s)	208, 344, 979, 1321, 1974, 2282, 2803, 3166
Tru1I	TTAA	5 cut(s)	869, 1011, 2583, 3336, 3364
Tru9I	TTAA	5 cut(s)	869, 1011, 2583, 3336, 3364
TscAI	CASTG	9 cut(s)	514, 1105, 1156, 1621, 2218, 2340, 2487, 2788, 3179
TseFI	GTSAC	6 cut(s)	352, 1631, 2213, 2482, 2497, 2555
TseI	GCWGC	5 cut(s)	5, 589, 1262, 1498, 2957
Tsp45I	GTSAC	6 cut(s)	352, 1631, 2213, 2482, 2497, 2555
TspDTI	ATGAA	6 cut(s)	437, 650, 1826, 1846, 1912, 2782
TspGWI	ACGGA	2 cut(s)	1895, 2603
TspRI	CASTG	9 cut(s)	514, 1105, 1156, 1621, 2218, 2340, 2487, 2788, 3179
Tth111I	GACNNNGTC	1 cut(s)	2185
Van91I	CCANNNNNTGG	1 cut(s)	235
VneI	GTGCAC	1 cut(s)	2043
VpaK11BI	GGWCC	2 cut(s)	2063, 2085
VspI	ATTAAT	2 cut(s)	1011, 2583
XagI	CCTNNNNNAGG	1 cut(s)	2978
XapI	RAATTY	6 cut(s)	554, 998, 1644, 2263, 2339, 3000
XbaI	TCTAGA	1 cut(s)	146
XceI	RCATGY	4 cut(s)	393, 628, 1209, 1762
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1816, 2950, 3247
XmiI	GTMKAC	2 cut(s)	1755, 2826
XmnI	GAANNNNTTC	1 cut(s)	118
XspI	CTAG	5 cut(s)	147, 1175, 1202, 1266, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.