Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008941

Physical Location & Seq

Genomic Coordinates Forward (+)

27606955 .. 27611453

Gene Structure 4499 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1144400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3399 bp

ATGGCAACTTCCCAGAATAAAGAACCGTCGAAGAAGGCGATAAAAAACCCTGGGGGTTCTGGTAGCTCTCAAGGCAAAACTAATTCGAGTGGCCAAACTGTTAAGTTTGCGCGAAGGACTTCAAGTGGACGGTATGTGAGTCTGTCAAGAGAAGACCTTGATATGTCCGGGGAATTATCTGGGGACTATATGAACTACACAGTTCATATTCCACCCACCCCGGATAACCAGCCCATGGACACATCCGTGGCTGTAAAGGCAGAGGAGCAATATGTTTCGAATTCTTTATTTACCGGAGGGTTCAATAGCGTGACTCGTGCACATCTCATGGATAAGGTGATTGATTCGGAGGTGACTCATCCTCAGATGGCTGGAGCCAAAGGCTCTGCATGCATGATGCCTGCTTGTGATGGTAAGGTGATGAAGGATGAGAGAGGAGTTGATATAACCCCTTGTGATTGCAGGTTCAAAATCTGCAGAGATTGCTATTTGGATGCACAGAAGGACACTGGCCTTTGTCCAGGCTGCAAGGAGCAATACAGAGTAGGAGACGAATATGATGAGCCATCGGATTACAACAGTGGAACGCTGCAATTGCCTGGTCCTGACGGAAAAAGGGATAACATGTCTGTGATGAAGAGGAACCAAACGGGAGAATTTGATCACAATAGGTGGTTGTTTGAGACAAAGGGGACTTATGGTGTTGGCAATGCTTTCAATCCCCAAGATGACGGGTATGGTGATGGCGGTGGTGATGGCTTCCCAGGGGGCTCGCTGGATGCAGATGACAAGCCCTGGAAGCCCCTCAGCAGGATATTGCCAATCCCTGCTGCCATTATCAGCCCCTACAGGTTACTGATCTTCGTTAGATTAATCGTGCTATCATTCTTCCTGCATTGGAGAGTAGTCAATCCAAACAATGATGCAAGATGGCTGTGGCTCATGTCAATTATCTGCGAAATATGGTTCGCCTTCTCTTGGATTCTTGATCAGACTCCAAAGTTTTTCCCCATTAATCGTCAGACCGATCTTGAAGTCCTCCACGACAAGTTTGACATGCCATCACCATCCAATCCAACGGGCCGGTCTGACCTCCCTGGCATTGACTTCTATGTATCGACTGCTGATCCTGACAAAGAGCCACCTCTCACCACTGCCAATACCATCCTTTCAATCCTAGCCGTTGATTACCCGGTTGAAAAGATAGCATGCTACATCTCTGATGATGGAGGTGCCCTCCTCACCTTCGAGGCAATGGCGGAGGCTGCTAGTTTCGCGGACTTGTGGGTCCCCTTCTGCCGGAAGCACGACATTGAGCCGAGGAATCCTGAGAGTTACTTCGCGTTGAAAGTTGACCCAACAAAGAACAAGAGTAGTCTGGACTTTGTGAAGGATAGGAGGAAGATCAAGAGGGAGTATGATGAGTTCAAGGTGAGGATCAACGGGCTTCCGGATTCGATCAGGAGGCGGTCTGATGCTTTCCATGCCAGGGAGGAAATGAAGCAGTTGAAGAATATGAGGGAGAATGGAACTGACCCTCTGGAGCAAGTCAAAGTCCCCAAGGCTACATGGATGGCTGATGGCACACATTGGCCTGGTACTTGGGCTGTTCCTTCCCATGACCATGCCAAAGGTGACCATTCCGGAATTCTTCAGGTGATGTTGAAGCCTCCTAGTCCCGACTCACTATTGGGAAGTGCCGATGATGACAAGCTCATAGATTTCACAGATGTGGATATACGCCTCCCGATGTTTGTCTACATGTCGCGAGAAAAGCGGCCGGGCTATGATCACAACAAGAAAGCTGGCGCCATGAATGCGCTGGTGAGAGCATCCGCCATCTTGTCGAACGGCCCTTTCATTCTCAACCTTGACTGTGATCACTACATCAACAACTGCAAAGCTATCCGTGAAGGGATGTGCTTCATGATGGACAGAGGTGGTGAAAACATCTGCTACATTCAGTTTCCTCAGAGATTCGAAGGAATTGATCCTTCTGATCGCTATGCCAATCACAACACCGTGTTTTTCGACGGCAATATGCGTGCGCTTGATGGTTTGCAGGGTCCGATGTATGTGGGAACCGGGACCATGTTCCGGCGTTTTGCCTTGTACGGTTTTGATCCACCAAATCCTGACAAGCTGCCGGTGAAGAAGGATACTGAGACACCAGGAGAGCCTTTGACACAGTCGACTATAGAACCTTTGACAGCCTGTGACTTTGACCCGGATCTTGACACCAATCTACTTCCCAAGCGTTTTGGAAATTCGACAATGCTGGCGGAATCCATACCAGTTGCTGAGTACCAAGGCCGACCCCTAGCTGATCATCCTGCAGTGAAATTTGGACGGCCTCCGGGCGTTCTCAGAGCTCCTCGTGATCCACTCGATGCCACAAGTGTTGCTGAAGCCGTGTCTTCCATTTCTTGCTGGTACGAGGACAAGACCGAATGGGGAGACCGTGTGGGGTGGATTTACGGGTCGGTGACAGAAGACGTGGTGACTGGGTACAGAATGCACAACCGAGGATGGCGCTCAGTGTACTGCGTTACCAAGCGTGACGCATTTCGAGGTTCAGCTCCCATTAATCTCACTGATCGACTCCACCAAGTGCTCCGTTGGGCAACAGGTTCTGTCGAAATTTTCTTCTCTCGGAACAATGCCTTCCTCGCCTCAATGCGCCTCAAATTACTACAGCGCCTTTCCTACGTCAATGTCGGTGTCTACCCTTTCACCTCGATATTTCTCATCGTGTACTGCTTCCTCCCAGCGCTCTCGCTCTTCACTGGACAGTTCATCGTGGCGAATCTCAACGTCACGTTTTTGATCTACTTGCTAACCATAACCATATGCCTCATTGCTCTGGCCCTCCTGGAGGTGAGGTGGTCGGGGGTCGCGTTGGAAGACTGGTGGCGAAACGAGCAGTTTTGGCTCATCTCCGGAACCAGCGCTCACTTGGCTGCTGTGGTGCAAGGGCTTCTAAAAGTGATGGCAGGGATTGAAATTTCCTTTACCTTGACAGCCAAGTCAGCTGGAGACGACAATGATGATATATATGCTGACCTCTACCTTGTGAAGTGGACTTCCCTCATGATCCCTCCAATTGTGATTGGAATGGTGAACATAATAGCCATTATCGTCGCATTTTCAAGGGAGGTTTATGCTCCGAATCCTCAGTGGGCGAGGTTTATCGGCGGTGCCTTCTTCAGCTTTTGGGTTTTGGCTCACTTGTATCCTTTTGCCAAGGGTTTGATGGGAAGAAGAAGGAAGACGCCTACCATTGTGTTTGTTTGGTCAGGTCTCATTGCCATTACACTTTCCTTGCTCTGGGTCGCCATTAACCCACCAGCTCCTGGTGCTGTCGCTGGCGCTGCAGGAGGCGGGTTCCAATTTCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000030 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008152 GO:0009846 GO:0009856 GO:0009987 GO:0012505 GO:0016740 GO:0016757 GO:0016758 GO:0019187 GO:0022414 GO:0032501 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0051704 GO:0051753 GO:0070085 GO:0071554 GO:0071669 GO:0097502

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1133

Amino Acids

126.31

Weight (kDa)

5.93

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	133 - 181	5.4e-15	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	368 - 716	1.1e-169	Cellulose synthase
Cellulose_synt	PF03552	745 - 1116	2.7e-187	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008941)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G38190
fragaria_vesca	FvH4_2g17120
malus_domestica	MD05G1144100.v1.1 MD05G1144400.v1.1 MD10G1143700.v1.1
prunus_persica	Prupe.8G182500_v2.0.a1
pyrus_communis	pycom05g13840 pycom05g13860 pycom05g13910 pycom05g13940 pycom10g12370
rosa_chinensis	RchiOBHm_Chr6g0281541
rosa_laevigata	RLG00000012965
rosa_multiflora	Rmu_sc0012649.1_g000003
rosa_roxburghii	Rroxscaffold_7G00187020
rosa_rugosa	Rorug06G0141300 Rorug06G0141400
rosa_samantha	Rh6AG253900 Rh6BG256800 Rh6CG256000 Rh6DG248000
rosa_wichuraiana	Rw6G022040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1286, 3025
Acc36I	ACCTGC	1 cut(s)	453
AccB1I	GGYRCC	3 cut(s)	1232, 1808, 3197
AccB7I	CCANNNNNTGG	2 cut(s)	235, 3353
AccI	GTMKAC	3 cut(s)	1758, 2192, 2724
AccII	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
AccIII	TCCGGA	3 cut(s)	1450, 1643, 2939
AciI	CCGC	9 cut(s)	747, 1259, 1277, 1468, 1777, 1836, 2282, 3195, 3381
AclWI	GGATC	7 cut(s)	1121, 1445, 1985, 2117, 2238, 2375, 3088
AcoI	YGGCCR	2 cut(s)	91, 1778
AcsI	RAATTY	7 cut(s)	280, 656, 1647, 2266, 2342, 2640, 3003
AcuI	CTGAAG	3 cut(s)	1637, 2427, 3190
AcyI	GRCGYC	2 cut(s)	1809, 3272
AdeI	CACNNNGTG	1 cut(s)	2611
AfaI	GTAC	7 cut(s)	1600, 2114, 2306, 2435, 2510, 2543, 2756
AfeI	AGCGCT	2 cut(s)	2772, 2950
AfiI	CCNNNNNNNGG	9 cut(s)	235, 810, 978, 1299, 1489, 2097, 2875, 3327, 3353
AflIII	ACRYGT	2 cut(s)	624, 1761
AjiI	CACGTC	1 cut(s)	2497
AleI	CACNNNNGTG	2 cut(s)	245, 1730
Alw21I	GWGCWC	3 cut(s)	322, 2374, 2616
Alw26I	GTCTC	6 cut(s)	543, 677, 2159, 2451, 3030, 3305
Alw44I	GTGCAC	1 cut(s)	318
AlwI	GGATC	7 cut(s)	1121, 1445, 1985, 2117, 2238, 2375, 3088
AlwNI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
Aor13HI	TCCGGA	3 cut(s)	1450, 1643, 2939
Aor51HI	AGCGCT	2 cut(s)	2772, 2950
AoxI	GGCC	9 cut(s)	91, 511, 1081, 1592, 1778, 1852, 2311, 2351, 2865
ApaLI	GTGCAC	1 cut(s)	318
ApeKI	GCWGC	7 cut(s)	525, 589, 830, 1265, 2143, 2960, 3371
ApoI	RAATTY	7 cut(s)	280, 656, 1647, 2266, 2342, 2640, 3003
AseI	ATTAAT	3 cut(s)	872, 1014, 2586
Asp700I	GAANNNNTTC	1 cut(s)	118
AspS9I	GGNCC	7 cut(s)	602, 1081, 1288, 1853, 2066, 2088, 2866
AsuC2I	CCSGG	7 cut(s)	169, 221, 1193, 1782, 2086, 2228, 2358
AsuII	TTCGAA	2 cut(s)	278, 1980
AvaII	GGWCC	4 cut(s)	602, 1288, 2066, 2088
BaeGI	GKGCMC	2 cut(s)	322, 1237
BalI	TGGCCA	1 cut(s)	93
BanI	GGYRCC	3 cut(s)	1232, 1808, 3197
BanII	GRGCYC	2 cut(s)	773, 2374
BauI	CACGAG	2 cut(s)	315, 2376
BbsI	GAAGAC	5 cut(s)	159, 2409, 2499, 2910, 3275
Bbv12I	GWGCWC	3 cut(s)	322, 2374, 2616
BbvCI	CCTCAGC	1 cut(s)	806
BbvI	GCAGC	7 cut(s)	512, 576, 817, 1252, 2130, 2947, 3358
BceAI	ACGGC	5 cut(s)	1166, 1867, 2050, 2366, 2396
BciVI	GTATCC	2 cut(s)	2152, 3243
BclI	TGATCA	5 cut(s)	661, 988, 1789, 1879, 2326
BcnI	CCSGG	7 cut(s)	169, 221, 1193, 1782, 2086, 2228, 2358
BcoDI	GTCTC	6 cut(s)	543, 677, 2159, 2451, 3030, 3305
BfaI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
BfmI	CTRYAG	6 cut(s)	475, 847, 2196, 2334, 2693, 3372
BfoI	RGCGCY	6 cut(s)	1812, 2536, 2701, 2774, 2952, 3372
BfuAI	ACCTGC	1 cut(s)	453
BfuI	GTATCC	2 cut(s)	2152, 3243
BisI	GCNGC	8 cut(s)	526, 590, 831, 1266, 1778, 2144, 2961, 3372
BlsI	GCNGC	8 cut(s)	527, 591, 832, 1267, 1779, 2145, 2962, 3373
Bme18I	GGWCC	4 cut(s)	602, 1288, 2066, 2088
BmgBI	CACGTC	1 cut(s)	2497
BmgT120I	GGNCC	7 cut(s)	602, 1081, 1288, 1853, 2066, 2088, 2866
BmrI	ACTGGG	1 cut(s)	2514
BmsI	GCATC	7 cut(s)	387, 484, 769, 913, 1465, 1841, 2380
BmuI	ACTGGG	1 cut(s)	2514
BpiI	GAAGAC	5 cut(s)	159, 2409, 2499, 2910, 3275
BplI	GAGNNNNNCTC	2 cut(s)	1221, 1253
BpmI	CTGGAG	4 cut(s)	393, 1562, 2894, 3054
Bpu10I	CCTNAGC	1 cut(s)	806
Bpu14I	TTCGAA	2 cut(s)	278, 1980
BpuEI	CTTGAG	1 cut(s)	54
BpuMI	CCSGG	7 cut(s)	169, 221, 1193, 1782, 2086, 2228, 2358
BsaHI	GRCGYC	2 cut(s)	1809, 3272
BsaI	GGTCTC	2 cut(s)	2451, 3305
BsaWI	WCCGGW	4 cut(s)	293, 1450, 1643, 2939
BsaXI	ACNNNNNCTCC	4 cut(s)	2448, 2478, 2867, 2897
Bsc4I	CCNNNNNNNGG	9 cut(s)	235, 810, 978, 1299, 1489, 2097, 2875, 3327, 3353
Bse118I	RCCGGY	2 cut(s)	1083, 2146
Bse1I	ACTGG	5 cut(s)	514, 2294, 2509, 2791, 2912
Bse3DI	GCAATG	4 cut(s)	715, 1260, 2856, 3303
BseAI	TCCGGA	3 cut(s)	1450, 1643, 2939
BseLI	CCNNNNNNNGG	9 cut(s)	235, 810, 978, 1299, 1489, 2097, 2875, 3327, 3353
BseMI	GCAATG	4 cut(s)	715, 1260, 2856, 3303
BseMII	CTCAG	9 cut(s)	377, 820, 1320, 1985, 2154, 2292, 2380, 2550, 3188
BseNI	ACTGG	5 cut(s)	514, 2294, 2509, 2791, 2912
BseRI	GAGGAG	4 cut(s)	278, 450, 1229, 2364
BseSI	GKGCMC	2 cut(s)	322, 1237
BseX3I	CGGCCG	1 cut(s)	1778
BseXI	GCAGC	7 cut(s)	512, 576, 817, 1252, 2130, 2947, 3358
BseYI	CCCAGC	1 cut(s)	2767
Bsh1236I	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
Bsh1285I	CGRYCG	1 cut(s)	1781
BshFI	GGCC	9 cut(s)	93, 513, 1083, 1594, 1780, 1854, 2313, 2353, 2867
BshNI	GGYRCC	3 cut(s)	1232, 1808, 3197
BsiEI	CGRYCG	1 cut(s)	1781
BsiHKAI	GWGCWC	3 cut(s)	322, 2374, 2616
BslFI	GGGAC	6 cut(s)	197, 706, 1274, 1541, 1662, 2101
BslI	CCNNNNNNNGG	9 cut(s)	235, 810, 978, 1299, 1489, 2097, 2875, 3327, 3353
BsmAI	GTCTC	6 cut(s)	543, 677, 2159, 2451, 3030, 3305
BsmBI	CGTCTC	2 cut(s)	543, 3030
BsmFI	GGGAC	6 cut(s)	197, 706, 1274, 1541, 1662, 2101
BsmI	GAATGC	2 cut(s)	1822, 2520
BsnI	GGCC	9 cut(s)	93, 513, 1083, 1594, 1780, 1854, 2313, 2353, 2867
Bso31I	GGTCTC	2 cut(s)	2451, 3305
Bsp119I	TTCGAA	2 cut(s)	278, 1980
Bsp1286I	GDGCHC	5 cut(s)	322, 773, 1237, 2374, 2616
Bsp13I	TCCGGA	3 cut(s)	1450, 1643, 2939
Bsp19I	CCATGG	1 cut(s)	234
Bsp68I	TCGCGA	1 cut(s)	1768
BspACI	CCGC	9 cut(s)	747, 1259, 1277, 1468, 1777, 1836, 2282, 3195, 3381
BspANI	GGCC	9 cut(s)	93, 513, 1083, 1594, 1780, 1854, 2313, 2353, 2867
BspCNI	CTCAG	9 cut(s)	376, 819, 1321, 1984, 2155, 2293, 2379, 2549, 3187
BspEI	TCCGGA	3 cut(s)	1450, 1643, 2939
BspFNI	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
BspHI	TCATGA	2 cut(s)	1926, 3090
BspMAI	CTGCAG	3 cut(s)	479, 2338, 3376
BspMI	ACCTGC	1 cut(s)	453
BspPI	GGATC	7 cut(s)	1121, 1445, 1985, 2117, 2238, 2375, 3088
BspQI	GCTCTTC	1 cut(s)	2786
BspT104I	TTCGAA	2 cut(s)	278, 1980
BspT107I	GGYRCC	3 cut(s)	1232, 1808, 3197
BspTNI	GGTCTC	2 cut(s)	2451, 3305
BsrDI	GCAATG	4 cut(s)	715, 1260, 2856, 3303
BsrFI	RCCGGY	2 cut(s)	1083, 2146
BsrI	ACTGG	5 cut(s)	514, 2294, 2509, 2791, 2912
BssAI	RCCGGY	2 cut(s)	1083, 2146
BssNI	GRCGYC	2 cut(s)	1809, 3272
BssSI	CACGAG	2 cut(s)	315, 2376
BssT1I	CCWWGG	4 cut(s)	234, 1560, 2308, 3243
Bst2BI	CACGAG	2 cut(s)	315, 2376
Bst6I	CTCTTC	2 cut(s)	632, 2786
BstACI	GRCGYC	2 cut(s)	1809, 3272
BstAPI	GCANNNNNTGC	1 cut(s)	483
BstBI	TTCGAA	2 cut(s)	278, 1980
BstC8I	GCNNGC	8 cut(s)	391, 402, 773, 1210, 1807, 2046, 2280, 3367
BstDEI	CTNAG	9 cut(s)	363, 806, 1329, 1971, 2163, 2301, 2366, 2536, 3174
BstDSI	CCRYGG	2 cut(s)	234, 246
BstEII	GGTNACC	1 cut(s)	1634
BstENI	CCTNNNNNAGG	1 cut(s)	2873
BstFNI	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
BstH2I	RGCGCY	6 cut(s)	1812, 2536, 2701, 2774, 2952, 3372
BstMAI	GTCTC	6 cut(s)	543, 677, 2159, 2451, 3030, 3305
BstMCI	CGRYCG	1 cut(s)	1781
BstNSI	RCATGY	5 cut(s)	393, 628, 1060, 1212, 1765
BstPI	GGTNACC	1 cut(s)	1634
BstSFI	CTRYAG	6 cut(s)	475, 847, 2196, 2334, 2693, 3372
BstSLI	GKGCMC	2 cut(s)	322, 1237
BstUI	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
BstV1I	GCAGC	7 cut(s)	512, 576, 817, 1252, 2130, 2947, 3358
BstV2I	GAAGAC	5 cut(s)	159, 2409, 2499, 2910, 3275
BstX2I	RGATCY	1 cut(s)	2230
BstYI	RGATCY	1 cut(s)	2230
BstZI	CGGCCG	1 cut(s)	1778
BsuI	GTATCC	2 cut(s)	2152, 3243
BsuRI	GGCC	9 cut(s)	93, 513, 1083, 1594, 1780, 1854, 2313, 2353, 2867
BtgI	CCRYGG	2 cut(s)	234, 246
BtrI	CACGTC	1 cut(s)	2497
BtsI	GCAGTG	2 cut(s)	1152, 2343
BtsIMutI	CAGTG	8 cut(s)	507, 586, 1152, 2343, 2544, 2592, 2784, 3182
BtuMI	TCGCGA	1 cut(s)	1768
BveI	ACCTGC	1 cut(s)	453
Cac8I	GCNNGC	8 cut(s)	391, 402, 773, 1210, 1807, 2046, 2280, 3367
CaiI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
CciI	TCATGA	2 cut(s)	1926, 3090
Cfr10I	RCCGGY	2 cut(s)	1083, 2146
Cfr13I	GGNCC	7 cut(s)	602, 1081, 1288, 1853, 2066, 2088, 2866
CseI	GACGC	2 cut(s)	2570, 3280
Csp6I	GTAC	7 cut(s)	1599, 2113, 2305, 2434, 2509, 2542, 2755
CspCI	CAANNNNNGTGG	2 cut(s)	2383, 2418
CviQI	GTAC	7 cut(s)	1599, 2113, 2305, 2434, 2509, 2542, 2755
DdeI	CTNAG	9 cut(s)	363, 806, 1329, 1971, 2163, 2301, 2366, 2536, 3174
DinI	GGCGCC	1 cut(s)	1810
DraIII	CACNNNGTG	1 cut(s)	2611
DrdI	GACNNNNNNGTC	2 cut(s)	1286, 3025
DseDI	GACNNNNNNGTC	2 cut(s)	1286, 3025
EaeI	YGGCCR	2 cut(s)	91, 1778
EagI	CGGCCG	1 cut(s)	1778
Eam1104I	CTCTTC	2 cut(s)	632, 2786
EarI	CTCTTC	2 cut(s)	632, 2786
EciI	GGCGGA	3 cut(s)	1274, 1825, 2297
Ecl136II	GAGCTC	1 cut(s)	2372
EclXI	CGGCCG	1 cut(s)	1778
Eco130I	CCWWGG	4 cut(s)	234, 1560, 2308, 3243
Eco24I	GRGCYC	2 cut(s)	773, 2374
Eco31I	GGTCTC	2 cut(s)	2451, 3305
Eco47I	GGWCC	4 cut(s)	602, 1288, 2066, 2088
Eco47III	AGCGCT	2 cut(s)	2772, 2950
Eco52I	CGGCCG	1 cut(s)	1778
Eco53kI	GAGCTC	1 cut(s)	2372
Eco57I	CTGAAG	3 cut(s)	1637, 2427, 3190
Eco91I	GGTNACC	1 cut(s)	1634
EcoICRI	GAGCTC	1 cut(s)	2372
EcoNI	CCTNNNNNAGG	1 cut(s)	2873
EcoO109I	RGGNCCY	1 cut(s)	1288
EcoO65I	GGTNACC	1 cut(s)	1634
EcoRI	GAATTC	2 cut(s)	280, 1647
EcoT14I	CCWWGG	4 cut(s)	234, 1560, 2308, 3243
EcoT22I	ATGCAT	1 cut(s)	395
EcoT38I	GRGCYC	2 cut(s)	773, 2374
EgeI	GGCGCC	1 cut(s)	1810
EheI	GGCGCC	1 cut(s)	1810
ErhI	CCWWGG	4 cut(s)	234, 1560, 2308, 3243
Esp3I	CGTCTC	2 cut(s)	543, 3030
FaqI	GGGAC	6 cut(s)	197, 706, 1274, 1541, 1662, 2101
FauI	CCCGC	1 cut(s)	3374
FauNDI	CATATG	1 cut(s)	2849
FbaI	TGATCA	5 cut(s)	661, 988, 1789, 1879, 2326
FblI	GTMKAC	3 cut(s)	1758, 2192, 2724
Fnu4HI	GCNGC	8 cut(s)	526, 590, 831, 1266, 1778, 2144, 2961, 3372
FriOI	GRGCYC	2 cut(s)	773, 2374
Fsp4HI	GCNGC	8 cut(s)	526, 590, 831, 1266, 1778, 2144, 2961, 3372
FspBI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
GluI	GCNGC	8 cut(s)	526, 590, 831, 1266, 1778, 2144, 2961, 3372
GsaI	CCCAGC	1 cut(s)	2771
GsuI	CTGGAG	4 cut(s)	393, 1562, 2894, 3054
HaeII	RGCGCY	6 cut(s)	1812, 2536, 2701, 2774, 2952, 3372
HaeIII	GGCC	9 cut(s)	93, 513, 1083, 1594, 1780, 1854, 2313, 2353, 2867
HgaI	GACGC	2 cut(s)	2570, 3280
Hin1I	GRCGYC	2 cut(s)	1809, 3272
HincII	GTYRAC	2 cut(s)	1354, 2193
HindII	GTYRAC	2 cut(s)	1354, 2193
Hpy99I	CGWCG	3 cut(s)	31, 2036, 3143
HpyCH4IV	ACGT	4 cut(s)	2496, 2709, 2814, 2819
HpyF3I	CTNAG	9 cut(s)	363, 806, 1329, 1971, 2163, 2301, 2366, 2536, 3174
HpySE526I	ACGT	4 cut(s)	2496, 2709, 2814, 2819
Hsp92I	GRCGYC	2 cut(s)	1809, 3272
KasI	GGCGCC	1 cut(s)	1808
KflI	GGGWCCC	1 cut(s)	1288
Kpn2I	TCCGGA	3 cut(s)	1450, 1643, 2939
Ksp22I	TGATCA	5 cut(s)	661, 988, 1789, 1879, 2326
LguI	GCTCTTC	1 cut(s)	2786
LmnI	GCTCC	9 cut(s)	265, 374, 532, 1543, 2377, 2584, 2619, 3169, 3355
Lsp1109I	GCAGC	7 cut(s)	512, 576, 817, 1252, 2130, 2947, 3358
LweI	GCATC	7 cut(s)	387, 484, 769, 913, 1465, 1841, 2380
MaeI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
MaeII	ACGT	4 cut(s)	2496, 2709, 2814, 2819
MfeI	CAATTG	2 cut(s)	593, 3102
MflI	RGATCY	1 cut(s)	2230
MhlI	GDGCHC	5 cut(s)	322, 773, 1237, 2374, 2616
MlsI	TGGCCA	1 cut(s)	93
MluNI	TGGCCA	1 cut(s)	93
Mly113I	GGCGCC	1 cut(s)	1809
MlyI	GAGTC	6 cut(s)	148, 307, 349, 988, 1676, 2595
MmeI	TCCRAC	2 cut(s)	1100, 2880
Mox20I	TGGCCA	1 cut(s)	93
Mph1103I	ATGCAT	1 cut(s)	395
MroI	TCCGGA	3 cut(s)	1450, 1643, 2939
MroXI	GAANNNNTTC	1 cut(s)	118
MscI	TGGCCA	1 cut(s)	93
MseI	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
MslI	CAYNNNNRTG	4 cut(s)	245, 629, 1623, 1730
Msp20I	TGGCCA	1 cut(s)	93
MspA1I	CMGCKG	1 cut(s)	3032
MunI	CAATTG	2 cut(s)	593, 3102
Mva1269I	GAATGC	2 cut(s)	1822, 2520
MvnI	CGCG	5 cut(s)	112, 1277, 1343, 1768, 2897
NarI	GGCGCC	1 cut(s)	1809
NciI	CCSGG	7 cut(s)	169, 221, 1193, 1782, 2086, 2228, 2358
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	2849
NmeAIII	GCCGAG	1 cut(s)	1344
NmuCI	GTSAC	8 cut(s)	310, 352, 1634, 2216, 2485, 2500, 2558, 2815
NruI	TCGCGA	1 cut(s)	1768
NsiI	ATGCAT	1 cut(s)	395
NspI	RCATGY	5 cut(s)	393, 628, 1060, 1212, 1765
NspV	TTCGAA	2 cut(s)	278, 1980
OliI	CACNNNNGTG	2 cut(s)	245, 1730
PaeI	GCATGC	2 cut(s)	393, 1212
PagI	TCATGA	2 cut(s)	1926, 3090
PasI	CCCWGGG	2 cut(s)	50, 764
PciI	ACATGT	2 cut(s)	624, 1761
PciSI	GCTCTTC	1 cut(s)	2786
PcsI	WCGNNNNNNNCGW	2 cut(s)	35, 2894
PctI	GAATGC	2 cut(s)	1822, 2520
PdmI	GAANNNNTTC	1 cut(s)	118
PfeI	GAWTC	8 cut(s)	344, 982, 1324, 1454, 1977, 2285, 2806, 3169
PflFI	GACNNNGTC	1 cut(s)	2188
PflMI	CCANNNNNTGG	2 cut(s)	235, 3353
PfoI	TCCNGGA	1 cut(s)	2871
PkrI	GCNGC	8 cut(s)	527, 591, 832, 1267, 1779, 2145, 2962, 3373
PleI	GAGTC	6 cut(s)	147, 307, 349, 988, 1676, 2595
PluTI	GGCGCC	1 cut(s)	1812
PpsI	GAGTC	6 cut(s)	147, 307, 349, 988, 1676, 2595
PpuMI	RGGWCCY	1 cut(s)	1288
PscI	ACATGT	2 cut(s)	624, 1761
PshBI	ATTAAT	3 cut(s)	872, 1014, 2586
Psp124BI	GAGCTC	1 cut(s)	2374
Psp5II	RGGWCCY	1 cut(s)	1288
PspEI	GGTNACC	1 cut(s)	1634
PspFI	CCCAGC	1 cut(s)	2767
PspPI	GGNCC	7 cut(s)	602, 1081, 1288, 1853, 2066, 2088, 2866
PspPPI	RGGWCCY	1 cut(s)	1288
PsrI	GAACNNNNNNTAC	2 cut(s)	1409, 1441
PstI	CTGCAG	3 cut(s)	479, 2338, 3376
PstNI	CAGNNNCTG	3 cut(s)	2300, 2633, 3353
PsuI	RGATCY	1 cut(s)	2230
PsyI	GACNNNGTC	1 cut(s)	2188
PvuII	CAGCTG	1 cut(s)	3032
RruI	TCGCGA	1 cut(s)	1768
RsaI	GTAC	7 cut(s)	1600, 2114, 2306, 2435, 2510, 2543, 2756
RsaNI	GTAC	7 cut(s)	1599, 2113, 2305, 2434, 2509, 2542, 2755
RseI	CAYNNNNRTG	4 cut(s)	245, 629, 1623, 1730
SacI	GAGCTC	1 cut(s)	2374
SalI	GTCGAC	1 cut(s)	2191
SapI	GCTCTTC	1 cut(s)	2786
SaqAI	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
SatI	GCNGC	8 cut(s)	526, 590, 831, 1266, 1778, 2144, 2961, 3372
Sau96I	GGNCC	7 cut(s)	602, 1081, 1288, 1853, 2066, 2088, 2866
SchI	GAGTC	6 cut(s)	148, 307, 349, 988, 1676, 2595
SduI	GDGCHC	5 cut(s)	322, 773, 1237, 2374, 2616
SfaNI	GCATC	7 cut(s)	387, 484, 769, 913, 1465, 1841, 2380
SfcI	CTRYAG	6 cut(s)	475, 847, 2196, 2334, 2693, 3372
SfoI	GGCGCC	1 cut(s)	1810
SfuI	TTCGAA	2 cut(s)	278, 1980
SinI	GGWCC	4 cut(s)	602, 1288, 2066, 2088
SmiMI	CAYNNNNRTG	4 cut(s)	245, 629, 1623, 1730
SmlI	CTYRAG	1 cut(s)	69
SmoI	CTYRAG	1 cut(s)	69
SphI	GCATGC	2 cut(s)	393, 1212
SsiI	CCGC	9 cut(s)	747, 1259, 1277, 1468, 1777, 1836, 2282, 3195, 3381
SspDI	GGCGCC	1 cut(s)	1808
SspMI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
SstI	GAGCTC	1 cut(s)	2374
StyI	CCWWGG	4 cut(s)	234, 1560, 2308, 3243
TaiI	ACGT	4 cut(s)	2499, 2712, 2817, 2822
TaqII	GACCGA	2 cut(s)	1040, 2462
TatI	WGTACW	2 cut(s)	2541, 2754
TauI	GCSGC	1 cut(s)	1780
TfiI	GAWTC	8 cut(s)	344, 982, 1324, 1454, 1977, 2285, 2806, 3169
Tru1I	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
Tru9I	TTAA	5 cut(s)	102, 872, 1014, 2586, 3339
TscAI	CASTG	8 cut(s)	514, 586, 1159, 2343, 2544, 2599, 2791, 3182
TseFI	GTSAC	8 cut(s)	310, 352, 1634, 2216, 2485, 2500, 2558, 2815
TseI	GCWGC	7 cut(s)	525, 589, 830, 1265, 2143, 2960, 3371
Tsp45I	GTSAC	8 cut(s)	310, 352, 1634, 2216, 2485, 2500, 2558, 2815
TspDTI	ATGAA	9 cut(s)	194, 206, 437, 650, 1514, 1829, 1849, 1915, 2785
TspGWI	ACGGA	5 cut(s)	235, 624, 1898, 2606, 3384
TspRI	CASTG	8 cut(s)	514, 586, 1159, 2343, 2544, 2599, 2791, 3182
Tth111I	GACNNNGTC	1 cut(s)	2188
Van91I	CCANNNNNTGG	2 cut(s)	235, 3353
VneI	GTGCAC	1 cut(s)	318
VpaK11BI	GGWCC	4 cut(s)	602, 1288, 2066, 2088
VspI	ATTAAT	3 cut(s)	872, 1014, 2586
XagI	CCTNNNNNAGG	1 cut(s)	2873
XapI	RAATTY	7 cut(s)	280, 656, 1647, 2266, 2342, 2640, 3003
XceI	RCATGY	5 cut(s)	393, 628, 1060, 1212, 1765
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1819, 2953, 3250
XmiI	GTMKAC	3 cut(s)	1758, 2192, 2724
XmnI	GAANNNNTTC	1 cut(s)	118
XspI	CTAG	4 cut(s)	1178, 1269, 1674, 2321
Zsp2I	ATGCAT	1 cut(s)	395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.