Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002955

Physical Location & Seq

Genomic Coordinates Forward (+)

30245000 .. 30258261

Gene Structure 13262 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1174400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6120 bp

ATGCATGGATGTAGCTCAGGATCTGCTCTCCTAGTAAATGCAGAGGTTGATTCCATGGGTGGAGTGGTTGACGGCGGAGTTGGTATTGGTTTGAAAACCTCTCCACGCCGAGCAGCAATCGAGAAGGCCCAAGCAGAGCTTAGACAGGAGTATGATGTGAGGGAGGAAAGAAGAAAGGAGCTAGAATTTCTTGAGAAAGGTGGCAATCCTTTGGACTTCAAGGTTGGGAATGGAGCTTCTGTTAGTGTTCAGTCTACTTCACTCACTGATCAGCAACCAGAACAGTTTGTGACCAGTGAAGCAAAAGGTAGTTTTGCATTGACTGCATCAACACATGGGGACTCTGTTGAAAGTAGCGGTCGACCAGAGGTTCCTACACTTTGTGAACCCAATAGTGCTGATAATCTCTTACTCTTTGATGCCGACAATGATGCTCCTGATGGTGAAAGGAATTCTATGCATCTTAGTAGAAGGAATAACAATGGTCCATCAGAACAGTCTTCCCAGATGGATGGGACTCAAAACCCCAAGGAATCAGAAGACTCGGCTATATTCCGTCCATATGCTCGAAGGAACAGGTCCAGACCAAATCGTGATGGCACTCGATCAAATTCAACAGATATGCAGGGTCGTGGAGGTCAGGGTTCTTCTTTACCTTCTCGTGGGCTGTCAAAGGATCCTAAGAGGCCGATATCTGAAACAAACAACCAAAAGGACCAGAATATACCTTCTGGCCCTAACGTAAAGTATGTGAGTTCAAATGGTGATATTGTTCCCAAGATTGTAACTTCTGATAATCAGTTCGATATGGACTTGGAGGGGGTGCAAGCCCCTGAAGTAATTACTGGTCCAATGAAAGATGGATCTCAAAACAAATTGGATGTTACAACTCTTAAAAGTTTGACGGATAGCCAACATAGTCAACCATCTCAGATTGATGCTCAGGAAACCCCCATTGATGTTGTTTCTGAGAGATCTGATGTTGTTGCAGACAGGGAACCACTAGCATCATCTGTCCTTGAGTGTCCTCCTTGTGCTGCTACAACAAAGACTGAAAATGAACTTAGTTCGGTTCAGATGAATGGGTATAACAATCTAAATAGGGAGAGTAAAAGTGTGCCACATGAAGGTCAAATTAGCAGTGCAGCTCTATGCCCAAAGGGTTTAGATTCAGAGTCTTCTTGCACCCAAACTAGTTTAGGATTAGATGTAAATAATGACAGTGACATGTGTACTACTATGAGGAACGCTGATAATGGAAATATTATCGAATCATCAGATGTGGATGGGACACGAAATCTAGCTGGTGGCATAATGGTACAAGAAGATAAGGAAACCAACGCTGTTGACAGTGGTGCTATTGTTAATGATAACCAGGCTTCTGTTTGTCAAAACAACTCTGGCAACAGCGAAGTCAAAGTTGAGGAAGACATGAGTCAAAGTAGATCTGAGTTGCATAATGAAGTTAAGCTTCATTCAAACGTTGAGGGAGAACAACCAAGTGACCCTCTAATATCAGAAGCTGTTAAGAAGGTGGATGAAGCTCTGGATAGTAGTTCCAACATCAACAAAGAGAACCTTTCCACTGGCATATCCCAGGGTCCTCAAGACTCATCAATGCATGAGGTTCCTGGGACTGTTTTGTCAGGGAAAGATACTGCTGCAGGTTCTGACTGCCAGACTCCTAGAGTTCACTTGAAAGTGGTAGACAAGGCACATGAAGATTCTATTCTGGAAAAGGCACGAATTATAGAGGCTAAGCGTAAGAGGATTGCTGAATTATCTGTTCGTTCTTTACCATCGGAGAATTGCCGAAAGTCTCAGTGGGATTTTGTCCTTGAAGAAATGGCTTGGTTGGCAAATGATTTTGCGCAGGAGCGTCTTTGGAAGCTTACTGCTGCTGCTCAAATTTGTCATCGCGCTGCTTTTACTTCTCGGTTGAGAATTGAATCCCAACATCAACATTGGGAGCTAAAAAAAGTGGCTCATGACCTGGCAAAGGCTGTCAACCAATTTTGGGTTTCAGCTGAGACCCTTTTGAAGGGTGATGATTCAAGTTCTTGTCAAAGGGACTGTAACTACGATTTGGTTGGATCAATGAGGATTGATAGGAACAAAACTTCTCAAGACAAGAATGGGGAACCCAATATGGAGCCAAGCAAAGATTTGGAACCACAGCATCCCCAAAAAGATCTTGCACTTTCAGTGCGGGAATACGCAGTGAGATTTTTGAAGTATAACAAATCTCTGGGTCCTGACCTTCAAGCACAAGCACCTGCTACTCCTGAGAGAATTTCTGATTTAGGCATTACAGAAATGTCATGGGAGGATCATCTTACAGAGGAAAATCTCTTCTATGCAGTTCCCTCTGGTGCAATGGAAACCTATAGAAAATTAATCGAATCTCATTTGGTTCAGTTTGAGACGAGAACTGGCAGTAGCATGCAAGAGGAAGTTGAGACATCTATGTATGATGCAGGAGCAGAGTTTGGTTTTCAAGAGGCTGCATATGATGAGGATGAAGGAGAAACAAGCACCTATTATTTGCCTGGAGCATTTGAAGGTAGCAAGTCATTAAAATCTAACCTGAAGAAACAAAAGAACTTGAAGTTATATGCTTCTAGGTCATATGAAGGAGCTGATTTGCCTTACGGCAACTGCACAACAGCAACTCACCAGTCCATGTTGATGGGGAAAAGGCCTGCTAGTCTCAATGTTGGTTCAATTCCAACGAAACGCATGCGCACTGCTTCCAGGCAGAGGGTCGTTAGTCCATTTGGTGCTGGAGCTAATGGGAATGTTCTGGGTCCGATTAAGACAGATGCTTCAAGTGGTGATACTAATTCTTTTCAGGATGATCAAAGTACTTTACATGGTGGATCTCAGTTCCAGAAAAGTGTGGAGGTTGAATCTGTTGGGGACTTTGAAAAGCAGTTACCATATGACTATGCAGAAACATCAATGAAGCCTAAAAAGAAGAAGAAGGCCAAACATCTGGGTTCTGCCTATGATCAAGGATGGCAGCTGGATTCTGCTATACTTAATGAACAGAGGGATCATTCCAAAAAGAGATCGGAGGGTCATCATTATGAATCCAATGGAACAATTGGTTTATACGGGCAACATACAGCCAAGAAGCCAAAGATATTAAAGCAATCTCTAGATAATACTTATGACAGTATTACTCCAATGCCTGGATCCAATCCTTCTCCAGTGGCATCCCAAATGAGTAATATGTCCAATACCAGTAAATTTATTAAGTTGATTGGTGGTCGAGACAGGGGACGGAAAACTAAATCACTAAAGATGTCTACTGGGCAGCCAGGTTCAGGAGGTCCATGGTCCCTTTTTGAAGATCAGGCACTTGTTGTTCTTGTACATGACATGGGCCCAAATTGGGAATTCATAAGTGATGCAATCAATAGCACGCTGCACTTGAAGTGTATTTTTCGCAAGCCTAAAGAGTGCAAGGAGCGTCACAAAATATTGATGGATATGAATTCTGGTGATGGGGCTGATAGTGCTGAAGATTCAGGATCTTCTCAACCTTATCCATCTACAATACCAGGCATTCCGAAGGGCAGTGCCCGACAATTATTTCAACGCTTGCAAGAGCCAATGGAAGAGGATGTTCTCAAATCTCATTTTGAGAAAATAATTAACATTGGTCAGAAGCATCATTATCGGAGGAGTCAGAATGAAAACCAGGATCCGAAACAAATAACGACTGTCCACAATTCTCATGTGGTGGCCCTTTCCCAAGTCTCTCCAAATAACTTGAATGGAGGTGTTCTAACGCCTCTTGATCTGTGTGATACCACTTCATCAAGCTCAGATGTCCTTGGGTATCAAGGATCTCATGCTAGTGGTTTAGCAATGTCAAATCAGGGTGCCATGGCGTCATTACTTCCTTCTGGGCCAAATGCCTCAATACAGGGATCTTCTGGTATGGTTCTTGGAAGCAACTTGCCATCCTCGTCTGGTCCACTTAGTGCCAACGTCAGAGATGGTAGATACAGTAGTCCAAGAACATCATCTTTGCCAGTTGATGAGCAGCAAAGACACTACAATCAAATGTTGTCTGGTAGAAACATTCAACAGTCTGGCTTGTCTGTGCCTGGAGCTCTTCCAGGAACTGATCGTGGAGTTCGTATGGTTCCTGGTGGAAATGGTATGGGCATGATGTGCGGGATAAACAGAGGCATGCCAGTGTCAAGGCCAGGATTTCAGGGAATGGCTTCATCATCTATGCTTAATTCTGGGAATATGCTTTCATCCAGTATGGTGGGGATACCAAGCCCTGTAAATATGCACTCTGGAGCTGGTTCGGGTCAGGGAAACTTGATGTTAAGACCTCGCGATGCTTTGCATATGATGCGGCCTGGCCATAATCCGGAGCATCAAAGGCAACTTATGGTTCCAGAACTTCAAATGCAGGCCACACAAGGGAATGGCCAAGGAGTTGCTCCCTTCAATGGGTTGAGTTCCGGTTTTCCTAATCAGCAAACTCCGCCATCTGCTCAAACATATCCAGGCCATAGCCCGCAGCAGCGTCAGATATCCCCACAACAGTCCCATGCACTCAGCAACCCTCATCACCCTCATCTTCAAGGCCCGAATCATGCTACGGGGTCGCAGCATCAAGCCTATGCCTTCCACGTCGCAAAAGAAAGGCAGTTGCAACAGCGGTATTTGCAGCAGCAGCAACAACAGCAACAGTTTTCTACATCTAATTCTTTGGTGCCACAGGTCCAGCCGCAGGCCCAACTTCCCATGTCATCAACTTTGCAAAATAGTTCCCAACTTCAGTCACAAACTTCACCTCACCCAGTATCTGTGTCCCCTATGACACCTTCTTCCCCAAGGACTCCCATGTCATCCCAGCACCAGCAGAAACATCATCTGCCATCCCATGGCTTTAGCCGGAATCCTGGTGCTAGTGGGATGACCAATCAGATAGGGAAGCAACGACAAAGACAACCACAGCAGCATCATCTTCAGCAATCTGGTAGGCACCACCCTCAGCAGCGGCAACTTACGCAGTCTCAGCAGCAGGCTAAACTTTCAAAGGGAATGGGAAGAGCCAATTCAATGGTGCATCAGAACCTCTCCATTGATCCTGCAAACCTCTCTATTGATTCTTCTCAACTTAATGGACTTGTGCCTCCAGGAAGCCAATCTCTTGAGAACGGAGAACAATTCATGCAATTGATGCAAGGTCAAGGTGCATATTCTGGGTCTGCCCTTAATCCAGCAACATCGAAACCATTGGTCCCCCAATCACCAAACCATTCTCAGTTGCAGCAAAAGTTACTTTCTAGTGCACCGACCAATTCATCAAAGCACCTCCAGCAAATGCCTTCTCATTCTGATAATATCACCCAGGGCCAGGTTCCACCAGTTCCTTCCAATCATACGATATCTGCTTCACATCAAACTGGCTCTCCATCAGGTATTGCTTCCAACCACCAGCAGCTGCAGCCGCAATCACAGGCACAGCAACAACAAAAGCAAGCTAATCAAACTCAGCCTTATGTTCAGAGAGTGCTGCAGCAGAATCACCAAGTGAATTTGGAGATTCCAAATAAGTCTCAAAATGATCTAACTCAAGGTGATGAGCAGCCTGTGAATGGTGCTTCGCCGGTAGGTGTGAGCACGGCGATTCCTCAGTCCTGTATTGATTCATCTAGTCTAGTTCCAGTTCCTTCTGCCATTCCTCAGTGGAAATCGTCAGAAGCAGTATATGATGCTAACATGCCGAATTCAACTGCTCAAGTGGGTCCAGTTGGGAGCCCCCCACTAACAAATTCATCTGGGAATGAGCCTGGGCCACCTATCAGTCAAGGGTTAGGCCCAAGAAGGTTATCTGGAAACTTGCTCTCTCATGGACTTAATGTTGGAGCACAGTGGCAGCAGCAGCAGCTACTGCAACGGTCTCCTTCGCTGCCATCCCCATCTCAACAACATTACCAGCAACAAGAACAACAGCAGCAGCAGCAGGAACAAGAGTCACCCCAACATCAACTGCCTCTGCAACAGCAGTCTCAGCAGCAAATGCAGCATCTGCAAGCAGGGCAAGGAAGTTTGTATACGATGTCTGGTAATTCAAAACCAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0000228 GO:0000785 GO:0000790 GO:0000812 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006464 GO:0006473 GO:0006475 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016569 GO:0016570 GO:0016573 GO:0016604 GO:0016607 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019538 GO:0031248 GO:0031974 GO:0031981 GO:0032991 GO:0035267 GO:0036211 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043968 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0051276 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0097346 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1904949 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HSA Myb_DNA-bind_6

Protein Analysis

2040

Amino Acids

221.53

Weight (kDa)

6.24

Isoelectric Point (pI)

60.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSA	PF07529	602 - 637	8.8e-06	HSA domain
Myb_DNA-bind_6	PF13921	1114 - 1162	6.6e-06	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24870 AT3G24870 AT3G24870 AT3G24870 AT3G24880 AT3G24880 AT3G24880
fragaria_vesca	FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860 FvH4_2g18860
malus_domestica	MD05G1174400.v1.1 MD10G1163500.v1.1
prunus_persica	Prupe.8G201800_v2.0.a1 Prupe.8G201800_v2.0.a1 Prupe.8G201800_v2.0.a1 Prupe.8G201800_v2.0.a1
pyrus_communis	pycom05g16120 pycom10g14100
rosa_chinensis	RchiOBHm_Chr6g0284671
rosa_laevigata	RLG00000012732
rosa_multiflora	Rmu_co8337863.1_g000001 Rmu_sc0007474.1_g000006
rosa_roxburghii	Rroxscaffold_7G00184490
rosa_rugosa	Rorug06G0165200 Rorug06G0165200 Rorug06G0165300 Rorug06G0165400
rosa_samantha	Rh6AG278300 Rh6BG278300 Rh6CG280600 Rh6DG273300
rosa_wichuraiana	Rw6G023920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2284
AasI	GACNNNNNNGTC	2 cut(s)	1642, 4873
Acc16I	TGCGCA	2 cut(s)	1872, 2744
Acc36I	ACCTGC	2 cut(s)	1655, 2284
AccB1I	GGYRCC	3 cut(s)	3890, 4741, 5013
AccB7I	CCANNNNNTGG	2 cut(s)	3194, 4913
AccI	GTMKAC	5 cut(s)	254, 361, 1707, 3311, 6089
AccII	CGCG	2 cut(s)	1920, 4359
AccIII	TCCGGA	1 cut(s)	4393
AclI	AACGTT	1 cut(s)	1482
AcoI	YGGCCR	2 cut(s)	4384, 4453
AcuI	CTGAAG	5 cut(s)	855, 2609, 3546, 4790, 4982
AcyI	GRCGYC	1 cut(s)	3899
AdeI	CACNNNGTG	3 cut(s)	383, 3992, 5566
AfaI	GTAC	4 cut(s)	1234, 1320, 2866, 3378
AflIII	ACRYGT	1 cut(s)	1227
AhlI	ACTAGT	1 cut(s)	1193
AjiI	CACGTC	1 cut(s)	4660
Alw21I	GWGCWC	4 cut(s)	4126, 5326, 5657, 5905
Alw44I	GTGCAC	1 cut(s)	5322
AlwNI	CAGNNNCTG	8 cut(s)	23, 1523, 1670, 4136, 4835, 5476, 5926, 6064
Aor13HI	TCCGGA	1 cut(s)	4393
ApaI	GGGCCC	1 cut(s)	3392
ApaLI	GTGCAC	1 cut(s)	5322
AseI	ATTAAT	1 cut(s)	2396
Asp700I	GAANNNNTTC	1 cut(s)	4237
AspLEI	GCGC	3 cut(s)	1873, 1922, 2745
BaeGI	GKGCMC	3 cut(s)	3392, 3589, 5326
BaeI	ACNNNNGTAYC	2 cut(s)	4006, 4039
BalI	TGGCCA	2 cut(s)	4386, 4455
BamHI	GGATCC	3 cut(s)	676, 3197, 3709
BanI	GGYRCC	3 cut(s)	3890, 4741, 5013
BanII	GRGCYC	3 cut(s)	3392, 4126, 5795
BarI	GAAGNNNNNNTAC	2 cut(s)	6073, 6105
BauI	CACGAG	1 cut(s)	660
BbsI	GAAGAC	4 cut(s)	492, 546, 1170, 1434
Bbv12I	GWGCWC	4 cut(s)	4126, 5326, 5657, 5905
BbvCI	CCTCAGC	1 cut(s)	5022
BceAI	ACGGC	3 cut(s)	88, 2668, 5673
BcgI	CGANNNNNNTGC	6 cut(s)	413, 447, 2722, 2756, 3665, 3699
BciVI	GTATCC	1 cut(s)	4284
BclI	TGATCA	3 cut(s)	268, 2857, 3010
BcuI	ACTAGT	1 cut(s)	1193
BfmI	CTRYAG	4 cut(s)	1662, 2386, 5477, 5549
BfuAI	ACCTGC	2 cut(s)	1655, 2284
BfuI	GTATCC	1 cut(s)	4284
BglII	AGATCT	3 cut(s)	974, 1445, 2191
BlpI	GCTNAGC	1 cut(s)	1758
BmcAI	AGTACT	1 cut(s)	2866
BmgBI	CACGTC	1 cut(s)	4660
BmrI	ACTGGG	2 cut(s)	3324, 4823
BmuI	ACTGGG	2 cut(s)	3324, 4823
BoxI	GACNNNNGTC	1 cut(s)	1434
BpiI	GAAGAC	4 cut(s)	492, 546, 1170, 1434
BpmI	CTGGAG	7 cut(s)	2571, 2805, 3195, 4140, 4338, 5151, 5333
Bpu10I	CCTNAGC	3 cut(s)	16, 942, 5022
Bpu1102I	GCTNAGC	1 cut(s)	1758
BpuEI	CTTGAG	7 cut(s)	212, 1040, 1590, 2109, 5204, 5592, 5757
BsaHI	GRCGYC	1 cut(s)	3899
BsaI	GGTCTC	2 cut(s)	2024, 5940
BsaWI	WCCGGW	2 cut(s)	4393, 4487
BsaXI	ACNNNNNCTCC	4 cut(s)	225, 255, 5428, 5458
Bse118I	RCCGGY	1 cut(s)	5641
Bse3DI	GCAATG	2 cut(s)	2382, 3882
BseAI	TCCGGA	1 cut(s)	4393
BseMI	GCAATG	2 cut(s)	2382, 3882
BseRI	GAGGAG	1 cut(s)	3703
BseSI	GKGCMC	3 cut(s)	3392, 3589, 5326
BseYI	CCCAGC	1 cut(s)	4881
BsgI	GTGCAG	3 cut(s)	1164, 2644, 3416
Bsh1236I	CGCG	2 cut(s)	1920, 4359
Bsh1285I	CGRYCG	1 cut(s)	361
BshNI	GGYRCC	3 cut(s)	3890, 4741, 5013
BsiEI	CGRYCG	1 cut(s)	361
BsiHKAI	GWGCWC	4 cut(s)	4126, 5326, 5657, 5905
BsiSI	CCGG	4 cut(s)	4394, 4488, 4924, 5642
BsmBI	CGTCTC	1 cut(s)	2417
BsmI	GAATGC	1 cut(s)	3570
Bso31I	GGTCTC	2 cut(s)	2024, 5940
Bsp120I	GGGCCC	1 cut(s)	3388
Bsp1286I	GDGCHC	7 cut(s)	3392, 3589, 4126, 5326, 5657, 5795, 5905
Bsp13I	TCCGGA	1 cut(s)	4393
Bsp1407I	TGTACA	1 cut(s)	3376
Bsp1720I	GCTNAGC	1 cut(s)	1758
Bsp19I	CCATGG	4 cut(s)	54, 3338, 3894, 4912
Bsp68I	TCGCGA	1 cut(s)	4359
BspEI	TCCGGA	1 cut(s)	4393
BspFNI	CGCG	2 cut(s)	1920, 4359
BspHI	TCATGA	1 cut(s)	1987
BspMAI	CTGCAG	3 cut(s)	1666, 5481, 5553
BspMI	ACCTGC	2 cut(s)	1655, 2284
BspQI	GCTCTTC	2 cut(s)	4131, 5074
BspT107I	GGYRCC	3 cut(s)	3890, 4741, 5013
BspTNI	GGTCTC	2 cut(s)	2024, 5940
BsrDI	GCAATG	2 cut(s)	2382, 3882
BsrFI	RCCGGY	1 cut(s)	5641
BsrGI	TGTACA	1 cut(s)	3376
BssAI	RCCGGY	1 cut(s)	5641
BssNAI	GTATAC	1 cut(s)	6090
BssNI	GRCGYC	1 cut(s)	3899
BssSI	CACGAG	1 cut(s)	660
BssT1I	CCWWGG	8 cut(s)	54, 528, 3338, 3841, 3894, 4456, 4862, 4912
Bst1107I	GTATAC	1 cut(s)	6090
Bst2BI	CACGAG	1 cut(s)	660
Bst6I	CTCTTC	4 cut(s)	2357, 3618, 4131, 5074
BstACI	GRCGYC	1 cut(s)	3899
BstAPI	GCANNNNNTGC	7 cut(s)	323, 4185, 4375, 5212, 5926, 6055, 6064
BstAUI	TGTACA	1 cut(s)	3376
BstDSI	CCRYGG	4 cut(s)	54, 3338, 3894, 4912
BstENI	CCTNNNNNAGG	2 cut(s)	1997, 2039
BstFNI	CGCG	2 cut(s)	1920, 4359
BstHHI	GCGC	3 cut(s)	1873, 1922, 2745
BstMCI	CGRYCG	1 cut(s)	361
BstNSI	RCATGY	5 cut(s)	1231, 2446, 2743, 4207, 5758
BstPAI	GACNNNNGTC	1 cut(s)	1434
BstSFI	CTRYAG	4 cut(s)	1662, 2386, 5477, 5549
BstSLI	GKGCMC	3 cut(s)	3392, 3589, 5326
BstUI	CGCG	2 cut(s)	1920, 4359
BstV2I	GAAGAC	4 cut(s)	492, 546, 1170, 1434
BstXI	CCANNNNNNTGG	5 cut(s)	512, 2689, 2995, 4285, 5092
BstZ17I	GTATAC	1 cut(s)	6090
BsuI	GTATCC	1 cut(s)	4284
BtgI	CCRYGG	4 cut(s)	54, 3338, 3894, 4912
BtgZI	GCGATG	2 cut(s)	1901, 4374
BtrI	CACGTC	1 cut(s)	4660
BtsI	GCAGTG	4 cut(s)	1147, 2226, 2745, 3589
BtuMI	TCGCGA	1 cut(s)	4359
BveI	ACCTGC	2 cut(s)	1655, 2284
CaiI	CAGNNNCTG	8 cut(s)	23, 1523, 1670, 4136, 4835, 5476, 5926, 6064
CciI	TCATGA	1 cut(s)	1987
CfoI	GCGC	3 cut(s)	1873, 1922, 2745
Cfr10I	RCCGGY	1 cut(s)	5641
CseI	GACGC	4 cut(s)	1868, 3464, 3888, 4541
Csp6I	GTAC	4 cut(s)	1233, 1319, 2865, 3377
CviQI	GTAC	4 cut(s)	1233, 1319, 2865, 3377
DraIII	CACNNNGTG	3 cut(s)	383, 3992, 5566
DrdI	GACNNNNNNGTC	2 cut(s)	1642, 4873
DseDI	GACNNNNNNGTC	2 cut(s)	1642, 4873
EaeI	YGGCCR	2 cut(s)	4384, 4453
Eam1104I	CTCTTC	4 cut(s)	2357, 3618, 4131, 5074
EarI	CTCTTC	4 cut(s)	2357, 3618, 4131, 5074
EciI	GGCGGA	2 cut(s)	90, 4500
Ecl136II	GAGCTC	1 cut(s)	4124
Eco130I	CCWWGG	8 cut(s)	54, 528, 3338, 3841, 3894, 4456, 4862, 4912
Eco147I	AGGCCT	1 cut(s)	2701
Eco24I	GRGCYC	3 cut(s)	3392, 4126, 5795
Eco31I	GGTCTC	2 cut(s)	2024, 5940
Eco32I	GATATC	3 cut(s)	693, 4560, 5421
Eco53kI	GAGCTC	1 cut(s)	4124
Eco57I	CTGAAG	5 cut(s)	855, 2609, 3546, 4790, 4982
EcoICRI	GAGCTC	1 cut(s)	4124
EcoNI	CCTNNNNNAGG	2 cut(s)	1997, 2039
EcoO109I	RGGNCCY	2 cut(s)	1601, 2252
EcoRI	GAATTC	4 cut(s)	451, 3401, 3499, 5761
EcoRV	GATATC	3 cut(s)	693, 4560, 5421
EcoT14I	CCWWGG	8 cut(s)	54, 528, 3338, 3841, 3894, 4456, 4862, 4912
EcoT22I	ATGCAT	3 cut(s)	6, 462, 1623
EcoT38I	GRGCYC	3 cut(s)	3392, 4126, 5795
ErhI	CCWWGG	8 cut(s)	54, 528, 3338, 3841, 3894, 4456, 4862, 4912
Esp3I	CGTCTC	1 cut(s)	2417
FalI	AAGNNNNNCTT	6 cut(s)	123, 155, 1455, 1487, 1563, 1595
FauI	CCCGC	3 cut(s)	2202, 4181, 4551
FauNDI	CATATG	5 cut(s)	562, 2509, 2629, 2941, 4371
FbaI	TGATCA	3 cut(s)	268, 2857, 3010
FblI	GTMKAC	5 cut(s)	254, 361, 1707, 3311, 6089
FriOI	GRGCYC	3 cut(s)	3392, 4126, 5795
FspAI	RTGCGCAY	1 cut(s)	2744
FspI	TGCGCA	2 cut(s)	1872, 2744
GlaI	GCGC	3 cut(s)	1872, 1921, 2744
GsaI	CCCAGC	1 cut(s)	4885
GsuI	CTGGAG	7 cut(s)	2571, 2805, 3195, 4140, 4338, 5151, 5333
HapII	CCGG	4 cut(s)	4394, 4488, 4924, 5642
HgaI	GACGC	4 cut(s)	1868, 3464, 3888, 4541
HhaI	GCGC	3 cut(s)	1873, 1922, 2745
Hin1I	GRCGYC	1 cut(s)	3899
Hin6I	GCGC	3 cut(s)	1871, 1920, 2743
HinP1I	GCGC	3 cut(s)	1871, 1920, 2743
HincII	GTYRAC	5 cut(s)	70, 362, 923, 1348, 2008
HindII	GTYRAC	5 cut(s)	70, 362, 923, 1348, 2008
HindIII	AAGCTT	2 cut(s)	1469, 1889
HpaII	CCGG	4 cut(s)	4394, 4488, 4924, 5642
Hpy99I	CGWCG	1 cut(s)	4664
HpyCH4IV	ACGT	4 cut(s)	741, 1482, 3999, 4659
HpySE526I	ACGT	4 cut(s)	741, 1482, 3999, 4659
Hsp92I	GRCGYC	1 cut(s)	3899
HspAI	GCGC	3 cut(s)	1871, 1920, 2743
Kpn2I	TCCGGA	1 cut(s)	4393
Ksp22I	TGATCA	3 cut(s)	268, 2857, 3010
LguI	GCTCTTC	2 cut(s)	4131, 5074
MaeII	ACGT	4 cut(s)	741, 1482, 3999, 4659
MfeI	CAATTG	2 cut(s)	3105, 5207
MhlI	GDGCHC	7 cut(s)	3392, 3589, 4126, 5326, 5657, 5795, 5905
MlsI	TGGCCA	2 cut(s)	4386, 4455
MluNI	TGGCCA	2 cut(s)	4386, 4455
MmeI	TCCRAC	5 cut(s)	1584, 2071, 2753, 5487, 5878
Mox20I	TGGCCA	2 cut(s)	4386, 4455
Mph1103I	ATGCAT	3 cut(s)	6, 462, 1623
MroI	TCCGGA	1 cut(s)	4393
MroXI	GAANNNNTTC	1 cut(s)	4237
MscI	TGGCCA	2 cut(s)	4386, 4455
MslI	CAYNNNNRTG	4 cut(s)	2687, 3087, 3864, 4208
Msp20I	TGGCCA	2 cut(s)	4386, 4455
MspA1I	CMGCKG	5 cut(s)	2027, 3025, 4687, 5029, 5476
MspI	CCGG	4 cut(s)	4394, 4488, 4924, 5642
MunI	CAATTG	2 cut(s)	3105, 5207
Mva1269I	GAATGC	1 cut(s)	3570
MvnI	CGCG	2 cut(s)	1920, 4359
NcoI	CCATGG	4 cut(s)	54, 3338, 3894, 4912
NdeI	CATATG	5 cut(s)	562, 2509, 2629, 2941, 4371
NmeAIII	GCCGAG	2 cut(s)	134, 524
NmuCI	GTSAC	6 cut(s)	289, 1223, 1502, 3476, 4809, 6009
NruI	TCGCGA	1 cut(s)	4359
NsbI	TGCGCA	2 cut(s)	1872, 2744
NsiI	ATGCAT	3 cut(s)	6, 462, 1623
NspI	RCATGY	5 cut(s)	1231, 2446, 2743, 4207, 5758
PaeI	GCATGC	3 cut(s)	2446, 2743, 4207
PagI	TCATGA	1 cut(s)	1987
PaqCI	CACCTGC	1 cut(s)	2284
PasI	CCCWGGG	2 cut(s)	1597, 5383
PceI	AGGCCT	1 cut(s)	2701
PciI	ACATGT	1 cut(s)	1227
PciSI	GCTCTTC	2 cut(s)	4131, 5074
PcsI	WCGNNNNNNNCGW	1 cut(s)	4147
PctI	GAATGC	1 cut(s)	3570
PdmI	GAANNNNTTC	1 cut(s)	4237
PflMI	CCANNNNNTGG	2 cut(s)	3194, 4913
PfoI	TCCNGGA	2 cut(s)	4129, 5167
PpuMI	RGGWCCY	2 cut(s)	1601, 2252
PscI	ACATGT	1 cut(s)	1227
PshAI	GACNNNNGTC	1 cut(s)	1434
PshBI	ATTAAT	1 cut(s)	2396
Psp124BI	GAGCTC	1 cut(s)	4126
Psp1406I	AACGTT	1 cut(s)	1482
Psp5II	RGGWCCY	2 cut(s)	1601, 2252
PspFI	CCCAGC	1 cut(s)	4881
PspOMI	GGGCCC	1 cut(s)	3388
PspPPI	RGGWCCY	2 cut(s)	1601, 2252
PstI	CTGCAG	3 cut(s)	1666, 5481, 5553
PstNI	CAGNNNCTG	8 cut(s)	23, 1523, 1670, 4136, 4835, 5476, 5926, 6064
PvuII	CAGCTG	3 cut(s)	2027, 3025, 5476
RruI	TCGCGA	1 cut(s)	4359
RsaI	GTAC	4 cut(s)	1234, 1320, 2866, 3378
RsaNI	GTAC	4 cut(s)	1233, 1319, 2865, 3377
RseI	CAYNNNNRTG	4 cut(s)	2687, 3087, 3864, 4208
SacI	GAGCTC	1 cut(s)	4126
SalI	GTCGAC	1 cut(s)	360
SapI	GCTCTTC	2 cut(s)	4131, 5074
ScaI	AGTACT	1 cut(s)	2866
SduI	GDGCHC	7 cut(s)	3392, 3589, 4126, 5326, 5657, 5795, 5905
SfcI	CTRYAG	4 cut(s)	1662, 2386, 5477, 5549
SmiMI	CAYNNNNRTG	4 cut(s)	2687, 3087, 3864, 4208
SmlI	CTYRAG	7 cut(s)	191, 1019, 1605, 2124, 5183, 5607, 5772
SmoI	CTYRAG	7 cut(s)	191, 1019, 1605, 2124, 5183, 5607, 5772
SpeI	ACTAGT	1 cut(s)	1193
SphI	GCATGC	3 cut(s)	2446, 2743, 4207
SseBI	AGGCCT	1 cut(s)	2701
SspI	AATATT	2 cut(s)	1264, 3486
SstI	GAGCTC	1 cut(s)	4126
StuI	AGGCCT	1 cut(s)	2701
StyI	CCWWGG	8 cut(s)	54, 528, 3338, 3841, 3894, 4456, 4862, 4912
TaiI	ACGT	4 cut(s)	744, 1485, 4002, 4662
TaqI	TCGA	9 cut(s)	120, 361, 568, 604, 804, 1269, 2400, 3274, 5261
TatI	WGTACW	3 cut(s)	1232, 2864, 3376
TauI	GCSGC	4 cut(s)	4381, 4759, 5032, 5485
TseFI	GTSAC	6 cut(s)	289, 1223, 1502, 3476, 4809, 6009
Tsp45I	GTSAC	6 cut(s)	289, 1223, 1502, 3476, 4809, 6009
TspGWI	ACGGA	4 cut(s)	545, 920, 3301, 5205
Van91I	CCANNNNNTGG	2 cut(s)	3194, 4913
VneI	GTGCAC	1 cut(s)	5322
VspI	ATTAAT	1 cut(s)	2396
XagI	CCTNNNNNAGG	2 cut(s)	1997, 2039
XbaI	TCTAGA	1 cut(s)	3160
XceI	RCATGY	5 cut(s)	1231, 2446, 2743, 4207, 5758
XcmI	CCANNNNNNNNNTGG	1 cut(s)	61
XmiI	GTMKAC	5 cut(s)	254, 361, 1707, 3311, 6089
XmnI	GAANNNNTTC	1 cut(s)	4237
ZrmI	AGTACT	1 cut(s)	2866
Zsp2I	ATGCAT	3 cut(s)	6, 462, 1623

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.