Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004431

Physical Location & Seq

Genomic Coordinates Reverse (-)

35549636 .. 35551144

Gene Structure 1509 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1223000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGTCTTCCCTTCACGTTTCCCCAAACACCATTTCTCAGACTCTCAATCTCCGCGGTCGATTCAGCCCCAACTTAGGCAGGGAAGTCCGACTAATCAACGTCCGGAGACCGGTCACTGGCTGCCTCAGCGTGGACGTGGAAGCACCCGATACCACCTCAAAACTAGAGATTTTGAAGAGCAACGAGGTTATGGAGATGGAGGCAAAGGTCCTGCTGCAGACCTACGCGAGGACTCCAGTGGTGCTCACCAGTGGCAAAGGTTGTAAATTGTACGACACCGAGGGGCGAGAGTATTTGGACCTGAGCTCGGGGATCGCGGTGAACGCGCTCGGCCATGGCGACGAGGACTGGTTGAAGGCTGTGGTTGATCAGGCCGGAACCCTCACTCACGTCAGCAATCTCTACTATTCGATCCCTCAGGTGGAGCTGGCAAAGCGTCTGGTGGGTGCTAGTTTTGCAGACCCCGGCCACGAGCTTCATATATCTTTCACAAACAGCTTCCATGGGAGGACCATGGGCGCTCTTGCCTTGACTAGCAAAGAGCATTACAGGTCCCCTTTTGAGCCTGTCATGCCTGGAGTCACGTTTGTGGAGTATGGAAATATACAGGCTACCAAAGAACTGATTCAGCCTGGAAAGACTGCCGCTGTTTTCGTTGAGCCGATTCAGGGTGAAGGGGGAATTTACAGTGCTTCAAAGGAGTTTCTGCAGTTTCTCCGTACTGCTTGTGATGATGCCGGTGCTCTTCTGGTATTTGACGAGATTCAATGTGGATTAGGTCGAACTGGCCATCTCTGGGCGCATGAGGCTTACGGTGTATTCCCAGATATCATGACACTGGCTAAGCCACTTGCTGGAGGACTTCCCATCGGAGCTGCATTGATGACTGNNNNN

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

Aminotran_3

Protein Analysis

298

Amino Acids

32.05

Weight (kDa)

5.72

Isoelectric Point (pI)

36.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_3	PF00202	77 - 151	3e-13	Aminotransferase class-III
Aminotran_3	PF00202	160 - 296	5.3e-46	Aminotransferase class-III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80600
fragaria_vesca	FvH4_3g15500 FvH4_3g15500 FvH4_6g29020
malus_domestica	MD05G1222800.v1.1 MD05G1223000.v1.1 MD10G1204400.v1.1 MD17G1219100.v1.1
prunus_persica	Prupe.3G094600_v2.0.a1 Prupe.4G137500_v2.0.a1
pyrus_communis	pycom05g20540
rosa_chinensis	RchiOBHm_Chr2g0133691 RchiOBHm_Chr5g0026121
rosa_laevigata	RLG00000019356 RLG00000032903
rosa_multiflora	Rmu_sc0000924.1_g000010 Rmu_sc0002806.1_g000006 Rmu_sc0002909.1_g000021 Rmu_sc0007285.1_g000005
rosa_roxburghii	Rroxscaffold_1G00053190 Rroxscaffold_2G00110900
rosa_rugosa	Rorug02G0313000 Rorug05G0090700
rosa_samantha	Rh2AG366400 Rh2BG372000 Rh2CG349500 Rh2DG388900 Rh5CG199400 Rh5DG182000
rosa_wichuraiana	Rw0G015720 Rw2G029810 Rw5G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	854
AccII	CGCG	4 cut(s)	54, 227, 317, 326
AccIII	TCCGGA	1 cut(s)	102
AciI	CCGC	4 cut(s)	52, 54, 317, 645
AclWI	GGATC	2 cut(s)	320, 406
AcoI	YGGCCR	3 cut(s)	331, 466, 787
AcsI	RAATTY	1 cut(s)	681
AfaI	GTAC	2 cut(s)	272, 721
AgeI	ACCGGT	1 cut(s)	109
AgsI	TTSAA	4 cut(s)	175, 355, 696, 767
AjiI	CACGTC	2 cut(s)	136, 391
AjnI	CCWGG	2 cut(s)	574, 631
AjuI	GAANNNNNNNTTGG	2 cut(s)	16, 48
AleI	CACNNNNGTG	1 cut(s)	587
AluBI	AGCT	5 cut(s)	306, 427, 475, 498, 875
AluI	AGCT	5 cut(s)	306, 427, 475, 498, 875
Alw21I	GWGCWC	3 cut(s)	246, 308, 745
Alw26I	GTCTC	1 cut(s)	100
AlwI	GGATC	2 cut(s)	320, 406
Ama87I	CYCGRG	1 cut(s)	307
Aor13HI	TCCGGA	1 cut(s)	102
AoxI	GGCC	4 cut(s)	331, 372, 466, 787
ApeKI	GCWGC	3 cut(s)	120, 214, 875
ApoI	RAATTY	1 cut(s)	681
AsiGI	ACCGGT	1 cut(s)	109
Asp700I	GAANNNNTTC	1 cut(s)	624
AspLEI	GCGC	3 cut(s)	328, 521, 802
AspS9I	GGNCC	4 cut(s)	208, 298, 510, 552
AsuC2I	CCSGG	1 cut(s)	465
AsuHPI	GGTGA	3 cut(s)	238, 331, 683
AvaI	CYCGRG	1 cut(s)	307
AvaII	GGWCC	4 cut(s)	208, 298, 510, 552
AxyI	CCTNAGG	1 cut(s)	417
BalI	TGGCCA	1 cut(s)	789
BanII	GRGCYC	1 cut(s)	308
BauI	CACGAG	1 cut(s)	470
Bbv12I	GWGCWC	3 cut(s)	246, 308, 745
BbvCI	CCTCAGC	1 cut(s)	125
BbvI	GCAGC	3 cut(s)	107, 201, 862
BccI	CCATC	3 cut(s)	190, 798, 875
BciT130I	CCWGG	2 cut(s)	576, 633
BclI	TGATCA	1 cut(s)	367
BcnI	CCSGG	1 cut(s)	465
BcoDI	GTCTC	1 cut(s)	100
BfaI	CTAG	3 cut(s)	164, 450, 534
BfmI	CTRYAG	2 cut(s)	215, 707
BfoI	RGCGCY	1 cut(s)	522
BisI	GCNGC	4 cut(s)	121, 215, 645, 876
BlpI	GCTNAGC	1 cut(s)	843
BlsI	GCNGC	4 cut(s)	122, 216, 646, 877
Bme1390I	CCNGG	3 cut(s)	465, 576, 633
Bme18I	GGWCC	4 cut(s)	208, 298, 510, 552
BmeT110I	CYCGRG	1 cut(s)	307
BmgBI	CACGTC	2 cut(s)	136, 391
BmgT120I	GGNCC	4 cut(s)	208, 298, 510, 552
BmiI	GGNNCC	2 cut(s)	379, 554
BmrFI	CCNGG	3 cut(s)	465, 576, 633
BmsI	GCATC	1 cut(s)	724
BpmI	CTGGAG	3 cut(s)	219, 597, 876
Bpu10I	CCTNAGC	2 cut(s)	125, 302
Bpu1102I	GCTNAGC	1 cut(s)	843
BpuMI	CCSGG	1 cut(s)	465
BsaI	GGTCTC	1 cut(s)	100
BsaJI	CCNNGG	6 cut(s)	52, 279, 334, 463, 502, 513
BsaWI	WCCGGW	2 cut(s)	102, 109
Bse118I	RCCGGY	2 cut(s)	109, 737
Bse1I	ACTGG	6 cut(s)	121, 236, 249, 353, 790, 843
Bse21I	CCTNAGG	1 cut(s)	417
BseAI	TCCGGA	1 cut(s)	102
BseBI	CCWGG	2 cut(s)	576, 633
BseDI	CCNNGG	6 cut(s)	52, 279, 334, 463, 502, 513
BseMII	CTCAG	4 cut(s)	50, 139, 293, 431
BseNI	ACTGG	6 cut(s)	121, 236, 249, 353, 790, 843
BseXI	GCAGC	3 cut(s)	107, 201, 862
Bsh1236I	CGCG	4 cut(s)	54, 227, 317, 326
Bsh1285I	CGRYCG	1 cut(s)	58
BshFI	GGCC	4 cut(s)	333, 374, 468, 789
BshTI	ACCGGT	1 cut(s)	109
BsiEI	CGRYCG	1 cut(s)	58
BsiHKAI	GWGCWC	3 cut(s)	246, 308, 745
BsiHKCI	CYCGRG	1 cut(s)	307
BsiSI	CCGG	5 cut(s)	103, 110, 375, 465, 738
BslFI	GGGAC	1 cut(s)	538
BsmAI	GTCTC	1 cut(s)	100
BsmFI	GGGAC	1 cut(s)	538
BsnI	GGCC	4 cut(s)	333, 374, 468, 789
Bso31I	GGTCTC	1 cut(s)	100
BsoBI	CYCGRG	1 cut(s)	307
Bsp1286I	GDGCHC	3 cut(s)	246, 308, 745
Bsp13I	TCCGGA	1 cut(s)	102
Bsp143I	GATC	3 cut(s)	312, 367, 411
Bsp1720I	GCTNAGC	1 cut(s)	843
Bsp19I	CCATGG	3 cut(s)	334, 502, 513
BspACI	CCGC	4 cut(s)	52, 54, 317, 645
BspANI	GGCC	4 cut(s)	333, 374, 468, 789
BspCNI	CTCAG	4 cut(s)	49, 138, 294, 430
BspEI	TCCGGA	1 cut(s)	102
BspFNI	CGCG	4 cut(s)	54, 227, 317, 326
BspHI	TCATGA	1 cut(s)	831
BspLI	GGNNCC	2 cut(s)	379, 554
BspMAI	CTGCAG	2 cut(s)	219, 711
BspPI	GGATC	2 cut(s)	320, 406
BspQI	GCTCTTC	2 cut(s)	170, 750
BspTNI	GGTCTC	1 cut(s)	100
BsrFI	RCCGGY	2 cut(s)	109, 737
BsrI	ACTGG	6 cut(s)	121, 236, 249, 353, 790, 843
BssAI	RCCGGY	2 cut(s)	109, 737
BssECI	CCNNGG	6 cut(s)	52, 279, 334, 463, 502, 513
BssMI	GATC	3 cut(s)	312, 367, 411
BssSI	CACGAG	1 cut(s)	470
BssT1I	CCWWGG	3 cut(s)	334, 502, 513
Bst2BI	CACGAG	1 cut(s)	470
Bst2UI	CCWGG	2 cut(s)	576, 633
Bst4CI	ACNGT	2 cut(s)	689, 815
Bst6I	CTCTTC	2 cut(s)	170, 750
BstC8I	GCNNGC	1 cut(s)	429
BstDEI	CTNAG	6 cut(s)	36, 73, 125, 302, 417, 843
BstDSI	CCRYGG	4 cut(s)	52, 334, 502, 513
BstENI	CCTNNNNNAGG	1 cut(s)	226
BstFNI	CGCG	4 cut(s)	54, 227, 317, 326
BstH2I	RGCGCY	1 cut(s)	522
BstHHI	GCGC	3 cut(s)	328, 521, 802
BstKTI	GATC	3 cut(s)	315, 370, 414
BstMAI	GTCTC	1 cut(s)	100
BstMBI	GATC	3 cut(s)	312, 367, 411
BstMCI	CGRYCG	1 cut(s)	58
BstMWI	GCNNNNNNNGC	6 cut(s)	126, 323, 433, 455, 571, 806
BstNI	CCWGG	2 cut(s)	576, 633
BstSCI	CCNGG	3 cut(s)	463, 574, 631
BstSFI	CTRYAG	2 cut(s)	215, 707
BstUI	CGCG	4 cut(s)	54, 227, 317, 326
BstV1I	GCAGC	3 cut(s)	107, 201, 862
Bsu36I	CCTNAGG	1 cut(s)	417
BsuRI	GGCC	4 cut(s)	333, 374, 468, 789
BtgI	CCRYGG	4 cut(s)	52, 334, 502, 513
BtrI	CACGTC	2 cut(s)	136, 391
BtsIMutI	CAGTG	5 cut(s)	114, 243, 256, 694, 836
Cac8I	GCNNGC	1 cut(s)	429
CciI	TCATGA	1 cut(s)	831
CfoI	GCGC	3 cut(s)	328, 521, 802
Cfr10I	RCCGGY	2 cut(s)	109, 737
Cfr13I	GGNCC	4 cut(s)	208, 298, 510, 552
Cfr42I	CCGCGG	1 cut(s)	55
CseI	GACGC	1 cut(s)	425
Csp6I	GTAC	2 cut(s)	271, 720
CspAI	ACCGGT	1 cut(s)	109
CviAII	CATG	6 cut(s)	335, 503, 514, 571, 803, 832
CviQI	GTAC	2 cut(s)	271, 720
DdeI	CTNAG	6 cut(s)	36, 73, 125, 302, 417, 843
DpnI	GATC	3 cut(s)	314, 369, 413
DpnII	GATC	3 cut(s)	312, 367, 411
EaeI	YGGCCR	3 cut(s)	331, 466, 787
Eam1104I	CTCTTC	2 cut(s)	170, 750
EarI	CTCTTC	2 cut(s)	170, 750
Ecl136II	GAGCTC	1 cut(s)	306
Eco130I	CCWWGG	3 cut(s)	334, 502, 513
Eco24I	GRGCYC	1 cut(s)	308
Eco31I	GGTCTC	1 cut(s)	100
Eco32I	GATATC	1 cut(s)	829
Eco47I	GGWCC	4 cut(s)	208, 298, 510, 552
Eco53kI	GAGCTC	1 cut(s)	306
Eco81I	CCTNAGG	1 cut(s)	417
Eco88I	CYCGRG	1 cut(s)	307
EcoICRI	GAGCTC	1 cut(s)	306
EcoNI	CCTNNNNNAGG	1 cut(s)	226
EcoO109I	RGGNCCY	2 cut(s)	208, 552
EcoRII	CCWGG	2 cut(s)	574, 631
EcoRV	GATATC	1 cut(s)	829
EcoT14I	CCWWGG	3 cut(s)	334, 502, 513
EcoT38I	GRGCYC	1 cut(s)	308
ErhI	CCWWGG	3 cut(s)	334, 502, 513
FaeI	CATG	6 cut(s)	338, 506, 517, 574, 806, 835
FaqI	GGGAC	1 cut(s)	538
FatI	CATG	6 cut(s)	334, 502, 513, 570, 802, 831
FbaI	TGATCA	1 cut(s)	367
Fnu4HI	GCNGC	4 cut(s)	121, 215, 645, 876
FriOI	GRGCYC	1 cut(s)	308
Fsp4HI	GCNGC	4 cut(s)	121, 215, 645, 876
FspBI	CTAG	3 cut(s)	164, 450, 534
GlaI	GCGC	3 cut(s)	327, 520, 801
GluI	GCNGC	4 cut(s)	121, 215, 645, 876
GsuI	CTGGAG	3 cut(s)	219, 597, 876
HaeII	RGCGCY	1 cut(s)	522
HaeIII	GGCC	4 cut(s)	333, 374, 468, 789
HapII	CCGG	5 cut(s)	103, 110, 375, 465, 738
HgaI	GACGC	1 cut(s)	425
HhaI	GCGC	3 cut(s)	328, 521, 802
Hin1II	CATG	6 cut(s)	338, 506, 517, 574, 806, 835
Hin6I	GCGC	3 cut(s)	326, 519, 800
HinP1I	GCGC	3 cut(s)	326, 519, 800
HinfI	GANTC	7 cut(s)	40, 60, 232, 579, 625, 664, 763
HpaII	CCGG	5 cut(s)	103, 110, 375, 465, 738
HphI	GGTGA	3 cut(s)	238, 331, 683
Hpy166II	GTNNAC	2 cut(s)	133, 322
Hpy188I	TCNGA	3 cut(s)	39, 89, 872
Hpy188III	TCNNGA	2 cut(s)	103, 832
Hpy8I	GTNNAC	2 cut(s)	133, 322
Hpy99I	CGWCG	1 cut(s)	344
HpyAV	CCTTC	3 cut(s)	20, 349, 668
HpyCH4III	ACNGT	2 cut(s)	689, 815
HpyCH4IV	ACGT	5 cut(s)	15, 99, 135, 390, 584
HpyCH4V	TGCA	4 cut(s)	217, 458, 709, 878
HpyF10VI	GCNNNNNNNGC	6 cut(s)	126, 323, 433, 455, 571, 806
HpyF3I	CTNAG	6 cut(s)	36, 73, 125, 302, 417, 843
HpySE526I	ACGT	5 cut(s)	15, 99, 135, 390, 584
Hsp92II	CATG	6 cut(s)	338, 506, 517, 574, 806, 835
HspAI	GCGC	3 cut(s)	326, 519, 800
Kpn2I	TCCGGA	1 cut(s)	102
Ksp22I	TGATCA	1 cut(s)	367
KspI	CCGCGG	1 cut(s)	55
Kzo9I	GATC	3 cut(s)	312, 367, 411
LguI	GCTCTTC	2 cut(s)	170, 750
LmnI	GCTCC	2 cut(s)	424, 872
Lsp1109I	GCAGC	3 cut(s)	107, 201, 862
LweI	GCATC	1 cut(s)	724
MaeI	CTAG	3 cut(s)	164, 450, 534
MaeII	ACGT	5 cut(s)	15, 99, 135, 390, 584
MaeIII	GTNAC	2 cut(s)	112, 580
MalI	GATC	3 cut(s)	314, 369, 413
MboI	GATC	3 cut(s)	312, 367, 411
MboII	GAAGA	2 cut(s)	187, 737
MhlI	GDGCHC	3 cut(s)	246, 308, 745
MlsI	TGGCCA	1 cut(s)	789
MluCI	AATT	2 cut(s)	266, 681
MluNI	TGGCCA	1 cut(s)	789
MlyI	GAGTC	3 cut(s)	34, 226, 588
MmeI	TCCRAC	1 cut(s)	112
Mox20I	TGGCCA	1 cut(s)	789
MroI	TCCGGA	1 cut(s)	102
MroXI	GAANNNNTTC	1 cut(s)	624
MscI	TGGCCA	1 cut(s)	789
MslI	CAYNNNNRTG	1 cut(s)	587
Msp20I	TGGCCA	1 cut(s)	789
MspA1I	CMGCKG	2 cut(s)	54, 647
MspI	CCGG	5 cut(s)	103, 110, 375, 465, 738
MspR9I	CCNGG	3 cut(s)	465, 576, 633
MvaI	CCWGG	2 cut(s)	576, 633
MvnI	CGCG	4 cut(s)	54, 227, 317, 326
MwoI	GCNNNNNNNGC	6 cut(s)	126, 323, 433, 455, 571, 806
NciI	CCSGG	1 cut(s)	465
NcoI	CCATGG	3 cut(s)	334, 502, 513
NdeII	GATC	3 cut(s)	312, 367, 411
NlaIII	CATG	6 cut(s)	338, 506, 517, 574, 806, 835
NlaIV	GGNNCC	2 cut(s)	379, 554
NmeAIII	GCCGAG	1 cut(s)	309
NmuCI	GTSAC	2 cut(s)	112, 580
OliI	CACNNNNGTG	1 cut(s)	587
PagI	TCATGA	1 cut(s)	831
PciSI	GCTCTTC	2 cut(s)	170, 750
PcsI	WCGNNNNNNNCGW	1 cut(s)	336
PdmI	GAANNNNTTC	1 cut(s)	624
PfeI	GAWTC	4 cut(s)	60, 625, 664, 763
PflMI	CCANNNNNTGG	1 cut(s)	854
PinAI	ACCGGT	1 cut(s)	109
PkrI	GCNGC	4 cut(s)	122, 216, 646, 877
PleI	GAGTC	3 cut(s)	34, 226, 587
PpsI	GAGTC	3 cut(s)	34, 226, 587
PpuMI	RGGWCCY	2 cut(s)	208, 552
Psp124BI	GAGCTC	1 cut(s)	308
Psp5II	RGGWCCY	2 cut(s)	208, 552
Psp6I	CCWGG	2 cut(s)	574, 631
PspGI	CCWGG	2 cut(s)	574, 631
PspN4I	GGNNCC	2 cut(s)	379, 554
PspPI	GGNCC	4 cut(s)	208, 298, 510, 552
PspPPI	RGGWCCY	2 cut(s)	208, 552
PstI	CTGCAG	2 cut(s)	219, 711
RsaI	GTAC	2 cut(s)	272, 721
RsaNI	GTAC	2 cut(s)	271, 720
RseI	CAYNNNNRTG	1 cut(s)	587
SacI	GAGCTC	1 cut(s)	308
SacII	CCGCGG	1 cut(s)	55
SapI	GCTCTTC	2 cut(s)	170, 750
SatI	GCNGC	4 cut(s)	121, 215, 645, 876
Sau3AI	GATC	3 cut(s)	312, 367, 411
Sau96I	GGNCC	4 cut(s)	208, 298, 510, 552
SchI	GAGTC	3 cut(s)	34, 226, 588
ScrFI	CCNGG	3 cut(s)	465, 576, 633
SduI	GDGCHC	3 cut(s)	246, 308, 745
SfaNI	GCATC	1 cut(s)	724
SfcI	CTRYAG	2 cut(s)	215, 707
Sfr303I	CCGCGG	1 cut(s)	55
SgrBI	CCGCGG	1 cut(s)	55
SinI	GGWCC	4 cut(s)	208, 298, 510, 552
SmiMI	CAYNNNNRTG	1 cut(s)	587
Sse9I	AATT	2 cut(s)	266, 681
SsiI	CCGC	4 cut(s)	52, 54, 317, 645
SspMI	CTAG	3 cut(s)	164, 450, 534
SstI	GAGCTC	1 cut(s)	308
StyD4I	CCNGG	3 cut(s)	463, 574, 631
StyI	CCWWGG	3 cut(s)	334, 502, 513
TaaI	ACNGT	2 cut(s)	689, 815
TaiI	ACGT	5 cut(s)	18, 102, 138, 393, 587
TaqI	TCGA	3 cut(s)	58, 410, 781
TasI	AATT	2 cut(s)	266, 681
TauI	GCSGC	1 cut(s)	647
TfiI	GAWTC	4 cut(s)	60, 625, 664, 763
TscAI	CASTG	5 cut(s)	121, 243, 256, 694, 843
TseFI	GTSAC	2 cut(s)	112, 580
TseI	GCWGC	3 cut(s)	120, 214, 875
Tsp45I	GTSAC	2 cut(s)	112, 580
TspDTI	ATGAA	1 cut(s)	467
TspGWI	ACGGA	1 cut(s)	707
TspRI	CASTG	5 cut(s)	121, 243, 256, 694, 843
Van91I	CCANNNNNTGG	1 cut(s)	854
VpaK11BI	GGWCC	4 cut(s)	208, 298, 510, 552
XagI	CCTNNNNNAGG	1 cut(s)	226
XapI	RAATTY	1 cut(s)	681
XmnI	GAANNNNTTC	1 cut(s)	624
XspI	CTAG	3 cut(s)	164, 450, 534

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.