Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0004431

Physical Location & Seq

Genomic Coordinates Reverse (-)

47213175 .. 47222064

Gene Structure 8890 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG349500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1062 bp

ATGGTAATGGTAAAGTACATATGCATCATAGCTTCTATATGCTTTTTTTTTTGTCCAAAAACTGGCTCAGGAAAATGGAGGTTGTTGTTGCAGGTGGAGCTGGCAAAACGGCTGGTGGCTTGCTCCTTTGCTGACCGTGTCTTTTTCTCAAACTCCGGAACTGAAGCAAATGAAGCTGCCATAAAATTTGCAAGAAAGTTCCAAAGATTTTCAAACCCTGTGAAGAAAAAGCCTACAACAGAATTCATTTCTTTCACTAACAGCTTCCATGGGAGGACGATGGGGTCCGTGGCTTTGACTAGTAAAGAGCATTACCGATCACCATTTGAGCCTCTCATGCCTGGAGTCACTTTCTTAGATTATGGAAATGTACAAGCTGCAAGAAAGCTAATTAAATCTGGCAAAATCGCTGCTGTGTTTGTTGAACCTATCCAGGGTGAAGGTGGGATAAATAGTGCAACTAAAGAATTTTTGCAATCCTTGCGTGGTGCTTGTGATGATTCAGGTTCTCTTCTGGTCTTTGATGAGGTTCAATGTGGGTTGGGTCGAACTGGTTATCTCTGGGCGCATGAAGCTTATGGTGTATTCCCAGATATAATGACTCTGGCCAAGCCTCTTGCAGGAGGACTTCCCATAGGAGCTGTATTAGTGACTGAAAAGGTTGCTGCTTCAATGAAGTATGGAGAGCATGGAAGCACTTTTGCTGGGGGGCCCCTTATCTGCAATGCTGCCCTTGCGGTCTTGGACAAAATTTCCAAGCCTGAGTTTCTCAAAGGCGTCTCAGAGAAAGGTCAGTACTTCAAAGAAGTGCTGACAAAGAAGCTGGGTACAAATCCACATGTGAGAGAAGTACGGGGTTTGGGTCTCATTGTTGGAGTAGAGCTGGACGTGTCTGCTTCACCGCTTGTCGATGCCTGTAGGAATTCTGGTCTTCTGATAATAACAGCCGGAAAAGGAAATGTCCTCAGGCTTGTGCCGCCGTTGATCATCTCTAAACCAGAATTGGAGCATGCAGCTGAGATTTTGCTTGAATGTTTGCCTGCCCTTGATGGCTCTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003992 GO:0005488 GO:0005507 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0008144 GO:0008150 GO:0008152 GO:0008483 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016053 GO:0016740 GO:0016769 GO:0019752 GO:0019842 GO:0022622 GO:0030170 GO:0032501 GO:0032502 GO:0036094 GO:0042742 GO:0042802 GO:0043167 GO:0043168 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044419 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0048037 GO:0048364 GO:0048731 GO:0048856 GO:0050662 GO:0050896 GO:0051704 GO:0051707 GO:0070279 GO:0071704 GO:0080022 GO:0097159 GO:0098542 GO:0099402 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

Aminotran_3

Protein Analysis

353

Amino Acids

38.02

Weight (kDa)

8.96

Isoelectric Point (pI)

26.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_3	PF00202	31 - 346	1.1e-95	Aminotransferase class-III

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80600
fragaria_vesca	FvH4_3g15500 FvH4_3g15500 FvH4_6g29020
malus_domestica	MD05G1222800.v1.1 MD05G1223000.v1.1 MD10G1204400.v1.1 MD17G1219100.v1.1
prunus_persica	Prupe.3G094600_v2.0.a1 Prupe.4G137500_v2.0.a1
pyrus_communis	pycom05g20540
rosa_chinensis	RchiOBHm_Chr2g0133691 RchiOBHm_Chr5g0026121
rosa_laevigata	RLG00000019356 RLG00000032903
rosa_multiflora	Rmu_sc0000924.1_g000010 Rmu_sc0002806.1_g000006 Rmu_sc0002909.1_g000021 Rmu_sc0007285.1_g000005
rosa_roxburghii	Rroxscaffold_1G00053190 Rroxscaffold_2G00110900
rosa_rugosa	Rorug02G0313000 Rorug05G0090700
rosa_samantha	Rh2AG366400 Rh2BG372000 Rh2CG349500 Rh2DG388900 Rh5CG199400 Rh5DG182000
rosa_wichuraiana	Rw0G015720 Rw2G029810 Rw5G016620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	82
AasI	GACNNNNNNGTC	1 cut(s)	283
Acc36I	ACCTGC	1 cut(s)	82
AccB7I	CCANNNNNTGG	1 cut(s)	62
AccIII	TCCGGA	1 cut(s)	155
AciI	CCGC	3 cut(s)	737, 902, 977
AcoI	YGGCCR	1 cut(s)	606
AcsI	RAATTY	5 cut(s)	185, 242, 467, 750, 922
AcuI	CTGAAG	1 cut(s)	183
AcyI	GRCGYC	1 cut(s)	777
AfaI	GTAC	5 cut(s)	17, 372, 797, 829, 852
AfiI	CCNNNNNNNGG	3 cut(s)	62, 434, 620
AflIII	ACRYGT	2 cut(s)	838, 888
AgsI	TTSAA	6 cut(s)	213, 425, 533, 672, 802, 1031
AhlI	ACTAGT	1 cut(s)	299
AjiI	CACGTC	1 cut(s)	889
AjnI	CCWGG	2 cut(s)	340, 432
Alw26I	GTCTC	2 cut(s)	784, 869
Aor13HI	TCCGGA	1 cut(s)	155
AoxI	GGCC	2 cut(s)	606, 710
ApaI	GGGCCC	1 cut(s)	714
ApeKI	GCWGC	6 cut(s)	176, 377, 410, 665, 728, 1013
ApoI	RAATTY	5 cut(s)	185, 242, 467, 750, 922
AspLEI	GCGC	1 cut(s)	568
AspS9I	GGNCC	3 cut(s)	285, 710, 711
AsuHPI	GGTGA	3 cut(s)	312, 449, 891
AvaII	GGWCC	1 cut(s)	285
AxyI	CCTNAGG	1 cut(s)	965
BaeGI	GKGCMC	1 cut(s)	714
BalI	TGGCCA	1 cut(s)	608
BanII	GRGCYC	1 cut(s)	714
BbsI	GAAGAC	1 cut(s)	923
BbvI	GCAGC	6 cut(s)	163, 364, 397, 652, 715, 1025
BccI	CCATC	2 cut(s)	274, 1043
BceAI	ACGGC	2 cut(s)	125, 964
BciT130I	CCWGG	2 cut(s)	342, 434
BclI	TGATCA	1 cut(s)	984
BcoDI	GTCTC	2 cut(s)	784, 869
BcuI	ACTAGT	1 cut(s)	299
BfaI	CTAG	1 cut(s)	300
BfmI	CTRYAG	1 cut(s)	916
BfuAI	ACCTGC	1 cut(s)	82
BisI	GCNGC	7 cut(s)	177, 378, 411, 666, 729, 977, 1014
BlsI	GCNGC	7 cut(s)	178, 379, 412, 667, 730, 978, 1015
BmcAI	AGTACT	1 cut(s)	797
Bme1390I	CCNGG	2 cut(s)	342, 434
Bme18I	GGWCC	1 cut(s)	285
BmgBI	CACGTC	1 cut(s)	889
BmgT120I	GGNCC	3 cut(s)	285, 710, 711
BmiI	GGNNCC	4 cut(s)	286, 711, 712, 713
BmrFI	CCNGG	2 cut(s)	342, 434
BmsI	GCATC	2 cut(s)	33, 901
BpiI	GAAGAC	1 cut(s)	923
BpmI	CTGGAG	1 cut(s)	363
Bpu10I	CCTNAGC	1 cut(s)	67
BsaHI	GRCGYC	1 cut(s)	777
BsaI	GGTCTC	1 cut(s)	869
BsaJI	CCNNGG	3 cut(s)	268, 288, 433
BsaWI	WCCGGW	1 cut(s)	155
Bsc4I	CCNNNNNNNGG	3 cut(s)	62, 434, 620
Bse1I	ACTGG	2 cut(s)	67, 556
Bse21I	CCTNAGG	1 cut(s)	965
Bse3DI	GCAATG	1 cut(s)	730
BseAI	TCCGGA	1 cut(s)	155
BseBI	CCWGG	2 cut(s)	342, 434
BseDI	CCNNGG	3 cut(s)	268, 288, 433
BseLI	CCNNNNNNNGG	3 cut(s)	62, 434, 620
BseMI	GCAATG	1 cut(s)	730
BseMII	CTCAG	5 cut(s)	81, 753, 795, 979, 1008
BseNI	ACTGG	2 cut(s)	67, 556
BseSI	GKGCMC	1 cut(s)	714
BseXI	GCAGC	6 cut(s)	163, 364, 397, 652, 715, 1025
BseYI	CCCAGC	2 cut(s)	704, 823
BshFI	GGCC	2 cut(s)	608, 712
BsiSI	CCGG	2 cut(s)	156, 948
BslI	CCNNNNNNNGG	3 cut(s)	62, 434, 620
BsmAI	GTCTC	2 cut(s)	784, 869
BsmBI	CGTCTC	1 cut(s)	784
BsnI	GGCC	2 cut(s)	608, 712
Bso31I	GGTCTC	1 cut(s)	869
Bsp120I	GGGCCC	1 cut(s)	710
Bsp1286I	GDGCHC	1 cut(s)	714
Bsp13I	TCCGGA	1 cut(s)	155
Bsp1407I	TGTACA	1 cut(s)	370
Bsp143I	GATC	2 cut(s)	317, 984
Bsp19I	CCATGG	1 cut(s)	268
BspACI	CCGC	3 cut(s)	737, 902, 977
BspANI	GGCC	2 cut(s)	608, 712
BspCNI	CTCAG	5 cut(s)	80, 754, 794, 978, 1009
BspEI	TCCGGA	1 cut(s)	155
BspLI	GGNNCC	4 cut(s)	286, 711, 712, 713
BspMI	ACCTGC	1 cut(s)	82
BspTNI	GGTCTC	1 cut(s)	869
BsrDI	GCAATG	1 cut(s)	730
BsrGI	TGTACA	1 cut(s)	370
BsrI	ACTGG	2 cut(s)	67, 556
BssECI	CCNNGG	3 cut(s)	268, 288, 433
BssMI	GATC	2 cut(s)	317, 984
BssNI	GRCGYC	1 cut(s)	777
BssT1I	CCWWGG	1 cut(s)	268
Bst2UI	CCWGG	2 cut(s)	342, 434
Bst4CI	ACNGT	1 cut(s)	137
Bst6I	CTCTTC	1 cut(s)	516
BstACI	GRCGYC	1 cut(s)	777
BstAPI	GCANNNNNTGC	1 cut(s)	481
BstAUI	TGTACA	1 cut(s)	370
BstC8I	GCNNGC	4 cut(s)	102, 121, 1011, 1041
BstDEI	CTNAG	6 cut(s)	67, 355, 762, 781, 965, 1017
BstDSI	CCRYGG	2 cut(s)	268, 288
BstENI	CCTNNNNNAGG	1 cut(s)	618
BstHHI	GCGC	1 cut(s)	568
BstKTI	GATC	2 cut(s)	320, 987
BstMAI	GTCTC	2 cut(s)	784, 869
BstMBI	GATC	2 cut(s)	317, 984
BstMWI	GCNNNNNNNGC	7 cut(s)	97, 173, 337, 481, 572, 734, 976
BstNI	CCWGG	2 cut(s)	342, 434
BstNSI	RCATGY	2 cut(s)	842, 1013
BstSCI	CCNGG	2 cut(s)	340, 432
BstSFI	CTRYAG	1 cut(s)	916
BstSLI	GKGCMC	1 cut(s)	714
BstV1I	GCAGC	6 cut(s)	163, 364, 397, 652, 715, 1025
BstV2I	GAAGAC	1 cut(s)	923
Bsu36I	CCTNAGG	1 cut(s)	965
BsuRI	GGCC	2 cut(s)	608, 712
BtgI	CCRYGG	2 cut(s)	268, 288
BtrI	CACGTC	1 cut(s)	889
BveI	ACCTGC	1 cut(s)	82
Cac8I	GCNNGC	4 cut(s)	102, 121, 1011, 1041
CfoI	GCGC	1 cut(s)	568
Cfr13I	GGNCC	3 cut(s)	285, 710, 711
CseI	GACGC	1 cut(s)	766
Csp6I	GTAC	5 cut(s)	16, 371, 796, 828, 851
CviAII	CATG	6 cut(s)	269, 337, 569, 689, 839, 1010
CviQI	GTAC	5 cut(s)	16, 371, 796, 828, 851
DdeI	CTNAG	6 cut(s)	67, 355, 762, 781, 965, 1017
DpnI	GATC	2 cut(s)	319, 986
DpnII	GATC	2 cut(s)	317, 984
DrdI	GACNNNNNNGTC	1 cut(s)	283
DseDI	GACNNNNNNGTC	1 cut(s)	283
EaeI	YGGCCR	1 cut(s)	606
Eam1104I	CTCTTC	1 cut(s)	516
EarI	CTCTTC	1 cut(s)	516
Eco130I	CCWWGG	1 cut(s)	268
Eco24I	GRGCYC	1 cut(s)	714
Eco31I	GGTCTC	1 cut(s)	869
Eco47I	GGWCC	1 cut(s)	285
Eco57I	CTGAAG	1 cut(s)	183
Eco81I	CCTNAGG	1 cut(s)	965
EcoNI	CCTNNNNNAGG	1 cut(s)	618
EcoO109I	RGGNCCY	2 cut(s)	710, 711
EcoRI	GAATTC	2 cut(s)	242, 922
EcoRII	CCWGG	2 cut(s)	340, 432
EcoT14I	CCWWGG	1 cut(s)	268
EcoT22I	ATGCAT	1 cut(s)	26
EcoT38I	GRGCYC	1 cut(s)	714
ErhI	CCWWGG	1 cut(s)	268
Esp3I	CGTCTC	1 cut(s)	784
FaeI	CATG	6 cut(s)	272, 340, 572, 692, 842, 1013
FatI	CATG	6 cut(s)	268, 336, 568, 688, 838, 1009
FauNDI	CATATG	1 cut(s)	20
FbaI	TGATCA	1 cut(s)	984
Fnu4HI	GCNGC	7 cut(s)	177, 378, 411, 666, 729, 977, 1014
FriOI	GRGCYC	1 cut(s)	714
Fsp4HI	GCNGC	7 cut(s)	177, 378, 411, 666, 729, 977, 1014
FspBI	CTAG	1 cut(s)	300
GlaI	GCGC	1 cut(s)	567
GluI	GCNGC	7 cut(s)	177, 378, 411, 666, 729, 977, 1014
GsaI	CCCAGC	2 cut(s)	708, 827
GsuI	CTGGAG	1 cut(s)	363
HaeIII	GGCC	2 cut(s)	608, 712
HapII	CCGG	2 cut(s)	156, 948
HgaI	GACGC	1 cut(s)	766
HhaI	GCGC	1 cut(s)	568
Hin1I	GRCGYC	1 cut(s)	777
Hin1II	CATG	6 cut(s)	272, 340, 572, 692, 842, 1013
Hin6I	GCGC	1 cut(s)	566
HinP1I	GCGC	1 cut(s)	566
HindIII	AAGCTT	1 cut(s)	573
HinfI	GANTC	3 cut(s)	345, 500, 601
HpaII	CCGG	2 cut(s)	156, 948
HphI	GGTGA	3 cut(s)	312, 449, 891
Hpy188I	TCNGA	2 cut(s)	784, 936
Hpy188III	TCNNGA	2 cut(s)	69, 156
HpyAV	CCTTC	1 cut(s)	434
HpyCH4III	ACNGT	1 cut(s)	137
HpyCH4IV	ACGT	1 cut(s)	888
HpyCH4V	TGCA	9 cut(s)	24, 91, 191, 380, 458, 475, 620, 723, 1013
HpyF10VI	GCNNNNNNNGC	7 cut(s)	97, 173, 337, 481, 572, 734, 976
HpyF3I	CTNAG	6 cut(s)	67, 355, 762, 781, 965, 1017
HpySE526I	ACGT	1 cut(s)	888
Hsp92I	GRCGYC	1 cut(s)	777
Hsp92II	CATG	6 cut(s)	272, 340, 572, 692, 842, 1013
HspAI	GCGC	1 cut(s)	566
Kpn2I	TCCGGA	1 cut(s)	155
Ksp22I	TGATCA	1 cut(s)	984
Kzo9I	GATC	2 cut(s)	317, 984
LmnI	GCTCC	4 cut(s)	97, 128, 638, 1006
Lsp1109I	GCAGC	6 cut(s)	163, 364, 397, 652, 715, 1025
LweI	GCATC	2 cut(s)	33, 901
MaeI	CTAG	1 cut(s)	300
MaeII	ACGT	1 cut(s)	888
MaeIII	GTNAC	2 cut(s)	346, 649
MalI	GATC	2 cut(s)	319, 986
MboI	GATC	2 cut(s)	317, 984
MboII	GAAGA	3 cut(s)	235, 503, 923
MhlI	GDGCHC	1 cut(s)	714
MlsI	TGGCCA	1 cut(s)	608
MluCI	AATT	7 cut(s)	185, 242, 390, 467, 750, 922, 1001
MluNI	TGGCCA	1 cut(s)	608
MlyI	GAGTC	2 cut(s)	354, 595
MmeI	TCCRAC	1 cut(s)	853
MnlI	CCTC	7 cut(s)	72, 267, 342, 520, 617, 624, 974
Mox20I	TGGCCA	1 cut(s)	608
Mph1103I	ATGCAT	1 cut(s)	26
MroI	TCCGGA	1 cut(s)	155
MscI	TGGCCA	1 cut(s)	608
MseI	TTAA	1 cut(s)	393
Msp20I	TGGCCA	1 cut(s)	608
MspA1I	CMGCKG	1 cut(s)	1016
MspI	CCGG	2 cut(s)	156, 948
MspR9I	CCNGG	2 cut(s)	342, 434
MvaI	CCWGG	2 cut(s)	342, 434
MwoI	GCNNNNNNNGC	7 cut(s)	97, 173, 337, 481, 572, 734, 976
NcoI	CCATGG	1 cut(s)	268
NdeI	CATATG	1 cut(s)	20
NdeII	GATC	2 cut(s)	317, 984
NlaIII	CATG	6 cut(s)	272, 340, 572, 692, 842, 1013
NlaIV	GGNNCC	4 cut(s)	286, 711, 712, 713
NmuCI	GTSAC	2 cut(s)	346, 649
NsiI	ATGCAT	1 cut(s)	26
NspI	RCATGY	2 cut(s)	842, 1013
PaeI	GCATGC	1 cut(s)	1013
PaqCI	CACCTGC	1 cut(s)	82
PciI	ACATGT	1 cut(s)	838
PfeI	GAWTC	1 cut(s)	500
PflFI	GACNNNGTC	1 cut(s)	137
PflMI	CCANNNNNTGG	1 cut(s)	62
PkrI	GCNGC	7 cut(s)	178, 379, 412, 667, 730, 978, 1015
PleI	GAGTC	2 cut(s)	353, 595
PpsI	GAGTC	2 cut(s)	353, 595
PscI	ACATGT	1 cut(s)	838
Psp6I	CCWGG	2 cut(s)	340, 432
PspFI	CCCAGC	2 cut(s)	704, 823
PspGI	CCWGG	2 cut(s)	340, 432
PspN4I	GGNNCC	4 cut(s)	286, 711, 712, 713
PspOMI	GGGCCC	1 cut(s)	710
PspPI	GGNCC	3 cut(s)	285, 710, 711
PsyI	GACNNNGTC	1 cut(s)	137
PvuII	CAGCTG	1 cut(s)	1016
RsaI	GTAC	5 cut(s)	17, 372, 797, 829, 852
RsaNI	GTAC	5 cut(s)	16, 371, 796, 828, 851
SaqAI	TTAA	1 cut(s)	393
SatI	GCNGC	7 cut(s)	177, 378, 411, 666, 729, 977, 1014
Sau3AI	GATC	2 cut(s)	317, 984
Sau96I	GGNCC	3 cut(s)	285, 710, 711
ScaI	AGTACT	1 cut(s)	797
SchI	GAGTC	2 cut(s)	354, 595
ScrFI	CCNGG	2 cut(s)	342, 434
SduI	GDGCHC	1 cut(s)	714
SfaNI	GCATC	2 cut(s)	33, 901
SfcI	CTRYAG	1 cut(s)	916
SinI	GGWCC	1 cut(s)	285
SpeI	ACTAGT	1 cut(s)	299
SphI	GCATGC	1 cut(s)	1013
Sse9I	AATT	7 cut(s)	185, 242, 390, 467, 750, 922, 1001
SsiI	CCGC	3 cut(s)	737, 902, 977
SspMI	CTAG	1 cut(s)	300
StyD4I	CCNGG	2 cut(s)	340, 432
StyI	CCWWGG	1 cut(s)	268
TaaI	ACNGT	1 cut(s)	137
TaiI	ACGT	1 cut(s)	891
TaqI	TCGA	2 cut(s)	547, 909
TasI	AATT	7 cut(s)	185, 242, 390, 467, 750, 922, 1001
TatI	WGTACW	3 cut(s)	15, 370, 795
TauI	GCSGC	1 cut(s)	979
TfiI	GAWTC	1 cut(s)	500
Tru1I	TTAA	1 cut(s)	393
Tru9I	TTAA	1 cut(s)	393
TseFI	GTSAC	2 cut(s)	346, 649
TseI	GCWGC	6 cut(s)	176, 377, 410, 665, 728, 1013
Tsp45I	GTSAC	2 cut(s)	346, 649
TspDTI	ATGAA	4 cut(s)	186, 235, 585, 689
TspGWI	ACGGA	1 cut(s)	277
Tth111I	GACNNNGTC	1 cut(s)	137
Van91I	CCANNNNNTGG	1 cut(s)	62
VpaK11BI	GGWCC	1 cut(s)	285
XagI	CCTNNNNNAGG	1 cut(s)	618
XapI	RAATTY	5 cut(s)	185, 242, 467, 750, 922
XceI	RCATGY	2 cut(s)	842, 1013
XcmI	CCANNNNNNNNNTGG	1 cut(s)	440
XspI	CTAG	1 cut(s)	300
ZrmI	AGTACT	1 cut(s)	797
Zsp2I	ATGCAT	1 cut(s)	26

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.