RLG00000032903

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004431
Physical Location & Seq
Forward (+)
20635636 .. 20639198
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032903

Sequence Viewer

Length: 1467 bp
ATGGCCATAATCGTGTCATGCCGCAGATTCTCCCAGATCTTCTCTCAGATTATAAGATCAAACTACGCTGAAATGACTTCCCTTCAAATCTCCCCCAACACAATTTCTCAACCTCTCAATCTCCGCAGTCAATTCAGCTCAAACTTGGACCAAAGGGTCCGACTAATCAATGGCCGGAGACCCGTGATGGCTTGCCTTAGCCTTGACGTGGAAGCACCAGTTACTGCATCAAAATCAGGGTTTTCGAAGAGCAAGGAGGTCAAAGAGACAGAGGCAAAGGTTCTGGTGGGGACATATGCAAGAACTCCAGTGGTGTTGGCCAGTGGCAAGGGTTGTAAATTGTACGACATAGAGGGGAGAGAGTATTTGGATTTGAGCTCAGGGATTGCGGTGAATGCACTTGGACATGGTGATGAGGACTGGTTGAAGGCTGTGGTTGAACAGGCCAATACCCTCACTCATGTCAGCAACGTCTACTACTCCATCCCTCAGGTGGAACTAGCAGAGCGTTTGGTGGGCTCTTGTTTTGCAGACCGTGTGTTCTTCACTAATTCTGGAACTGAAGCAAATGAAGCAGCCATTAAATTTGCGAGGAAGTTTCAGAGACACAATCATCCTGATGCGAAAGAGCCTGCGACAAACTTCATATCTTTCACTAACAGCTTCCATGGCAGGACCATGGGTGCGCTTGCCTTGACTAGCAAAGAGTATTACAGGTCTCCATTTGAGCCCGTCATGCCTGGAGTCACATTTGCAGAGTATGGAAATATAGAAGATACCAGAAAATTGATTCAGCCTGGAAAAACTGCTGCGGTTTTTGTTGAACCTATCCAGGGTGAAGGGGGCATATACAGTGCTACCAAGGAGTTTCTGCAATTTCTGCGTACTGCTTGTGATGAGGCTGGGGCTCTTCTGGTGTTCGACGAGGTTCAATGTGGTTTAGGCCGAACTGGGTATCTGTGGGCGCATGAGGCATTTGGTGTGTTCCCAGATATAATGACACTAGCTAAGCCACTAGCTGGAGGCCTACCCATTGGAGCTGCATTGGTGACTGAAAGAGTTGCTTCTACCATAGCTTTCGGTGACCATGGAAGTACTTTTGCTGGTGCACCTCTCGTGTGCAATGCTGCCTTAGCTGTTCTCAAAAAGATATCAAATCCCAATTTTCTGGACTGTGTCTCTAAGAAAGGTGTCTACTTCAAACATATCTTAAATCAGAAGCTGGGAGGAAACCCTCATGTGAGAGAAATACGCAGTTTTGGGCTTATTATTGGAGTTGAATTGGATGTCCCTGCCTCACCTCTTGTGGATGCATGTAGAAGTGCCGGCCTTCTAGTGTTAACTGCTGGAAACGGCAATGTTGTGAGGCTAGTGCCTCCATTGATCATAAGTGAGCAGGAGCTTGATCGTGCAGCTGAGATTTTGTACCAATCGTTGCCTGTACTTGACGAAAAAAATTCAAATTAG

Protein Analysis

489

Amino Acids

52.97

Weight (kDa)

6.77

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 102 - 477 9.6e-113 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 53
AasI GACNNNNNNGTC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 1019
AccI GTMKAC 2 cut(s) 474, 1194
AciI CCGC 4 cut(s) 22, 124, 389, 812
AcoI YGGCCR 3 cut(s) 3, 172, 318
AcsI RAATTY 2 cut(s) 584, 1456
AcuI CTGAAG 1 cut(s) 582
AfaI GTAC 5 cut(s) 344, 886, 1096, 1427, 1443
AfiI CCNNNNNNNGG 4 cut(s) 208, 493, 833, 1019
AgsI TTSAA 8 cut(s) 86, 427, 440, 824, 932, 1201, 1280, 1461
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 3 cut(s) 739, 796, 831
Alw21I GWGCWC 2 cut(s) 380, 1111
Alw26I GTCTC 5 cut(s) 172, 260, 598, 723, 1183
Alw44I GTGCAC 1 cut(s) 1107
AlwNI CAGNNNCTG 2 cut(s) 224, 1222
AoxI GGCC 7 cut(s) 3, 172, 318, 444, 943, 1024, 1327
ApaLI GTGCAC 1 cut(s) 1107
ApeKI GCWGC 5 cut(s) 575, 809, 1040, 1127, 1412
ApoI RAATTY 2 cut(s) 584, 1456
AspLEI GCGC 2 cut(s) 688, 967
AspS9I GGNCC 3 cut(s) 148, 157, 675
AsuHPI GGTGA 6 cut(s) 403, 422, 848, 1060, 1094, 1290
AsuII TTCGAA 1 cut(s) 245
AvaII GGWCC 3 cut(s) 148, 157, 675
AxyI CCTNAGG 1 cut(s) 489
BaeGI GKGCMC 1 cut(s) 1111
BalI TGGCCA 2 cut(s) 5, 320
BanII GRGCYC 4 cut(s) 380, 521, 732, 910
BauI CACGAG 1 cut(s) 1115
Bbv12I GWGCWC 2 cut(s) 380, 1111
BbvI GCAGC 5 cut(s) 587, 796, 1027, 1114, 1424
BccI CCATC 2 cut(s) 181, 491
BceAI ACGGC 1 cut(s) 1369
BciT130I CCWGG 3 cut(s) 741, 798, 833
BclI TGATCA 1 cut(s) 1383
BcoDI GTCTC 5 cut(s) 172, 260, 598, 723, 1183
BfaI CTAG 6 cut(s) 500, 699, 1004, 1016, 1334, 1370
BglII AGATCT 1 cut(s) 36
BisI GCNGC 6 cut(s) 22, 576, 810, 1041, 1128, 1413
BlpI GCTNAGC 1 cut(s) 1008
BlsI GCNGC 6 cut(s) 23, 577, 811, 1042, 1129, 1414
BmcAI AGTACT 1 cut(s) 1096
Bme1390I CCNGG 3 cut(s) 741, 798, 833
Bme18I GGWCC 3 cut(s) 148, 157, 675
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 148, 157, 675
BmiI GGNNCC 1 cut(s) 158
BmrFI CCNGG 3 cut(s) 741, 798, 833
BmrI ACTGGG 1 cut(s) 960
BmsI GCATC 3 cut(s) 236, 610, 1300
BmuI ACTGGG 1 cut(s) 960
BpmI CTGGAG 3 cut(s) 291, 762, 1041
Bpu10I CCTNAGC 3 cut(s) 197, 379, 1132
Bpu1102I GCTNAGC 1 cut(s) 1008
Bpu14I TTCGAA 1 cut(s) 245
BsaI GGTCTC 2 cut(s) 172, 723
BsaJI CCNNGG 5 cut(s) 667, 678, 832, 861, 1087
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 493, 833, 1019
Bse118I RCCGGY 1 cut(s) 1325
Bse1I ACTGG 5 cut(s) 218, 308, 321, 425, 955
Bse21I CCTNAGG 1 cut(s) 489
Bse3DI GCAATG 2 cut(s) 1129, 1363
BseBI CCWGG 3 cut(s) 741, 798, 833
BseDI CCNNGG 5 cut(s) 667, 678, 832, 861, 1087
BseGI GGATG 4 cut(s) 483, 613, 1291, 1315
BseLI CCNNNNNNNGG 4 cut(s) 208, 493, 833, 1019
BseMI GCAATG 2 cut(s) 1129, 1363
BseMII CTCAG 4 cut(s) 59, 393, 503, 1407
BseNI ACTGG 5 cut(s) 218, 308, 321, 425, 955
BseSI GKGCMC 1 cut(s) 1111
BseXI GCAGC 5 cut(s) 587, 796, 1027, 1114, 1424
BseYI CCCAGC 2 cut(s) 902, 1222
BsgI GTGCAG 1 cut(s) 1431
BshFI GGCC 7 cut(s) 5, 174, 320, 446, 945, 1026, 1329
BsiHKAI GWGCWC 2 cut(s) 380, 1111
BsiSI CCGG 2 cut(s) 175, 1326
BslFI GGGAC 2 cut(s) 304, 1274
BslI CCNNNNNNNGG 4 cut(s) 208, 493, 833, 1019
BsmAI GTCTC 5 cut(s) 172, 260, 598, 723, 1183
BsmFI GGGAC 2 cut(s) 304, 1274
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 7 cut(s) 5, 174, 320, 446, 945, 1026, 1329
Bso31I GGTCTC 2 cut(s) 172, 723
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 5 cut(s) 380, 521, 732, 910, 1111
Bsp143I GATC 4 cut(s) 36, 56, 1383, 1405
Bsp1720I GCTNAGC 1 cut(s) 1008
Bsp19I CCATGG 3 cut(s) 667, 678, 1087
BspACI CCGC 4 cut(s) 22, 124, 389, 812
BspANI GGCC 7 cut(s) 5, 174, 320, 446, 945, 1026, 1329
BspCNI CTCAG 4 cut(s) 58, 392, 502, 1408
BspLI GGNNCC 1 cut(s) 158
BspQI GCTCTTC 2 cut(s) 242, 915
BspT104I TTCGAA 1 cut(s) 245
BspTNI GGTCTC 2 cut(s) 172, 723
BsrDI GCAATG 2 cut(s) 1129, 1363
BsrFI RCCGGY 1 cut(s) 1325
BsrI ACTGG 5 cut(s) 218, 308, 321, 425, 955
BssAI RCCGGY 1 cut(s) 1325
BssECI CCNNGG 5 cut(s) 667, 678, 832, 861, 1087
BssMI GATC 4 cut(s) 36, 56, 1383, 1405
BssSI CACGAG 1 cut(s) 1115
BssT1I CCWWGG 4 cut(s) 667, 678, 861, 1087
Bst2BI CACGAG 1 cut(s) 1115
Bst2UI CCWGG 3 cut(s) 741, 798, 833
Bst4CI ACNGT 3 cut(s) 536, 854, 1175
Bst6I CTCTTC 2 cut(s) 242, 915
BstAPI GCANNNNNTGC 1 cut(s) 880
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 4 cut(s) 193, 633, 690, 1327
BstDEI CTNAG 8 cut(s) 45, 197, 379, 489, 1008, 1132, 1182, 1416
BstDSI CCRYGG 3 cut(s) 667, 678, 1087
BstEII GGTNACC 1 cut(s) 1082
BstF5I GGATG 4 cut(s) 483, 613, 1291, 1315
BstHHI GCGC 2 cut(s) 688, 967
BstKTI GATC 4 cut(s) 39, 59, 1386, 1408
BstMAI GTCTC 5 cut(s) 172, 260, 598, 723, 1183
BstMBI GATC 4 cut(s) 36, 56, 1383, 1405
BstMWI GCNNNNNNNGC 7 cut(s) 395, 572, 669, 736, 880, 971, 1133
BstNI CCWGG 3 cut(s) 741, 798, 833
BstNSI RCATGY 1 cut(s) 1317
BstPI GGTNACC 1 cut(s) 1082
BstSCI CCNGG 3 cut(s) 739, 796, 831
BstSLI GKGCMC 1 cut(s) 1111
BstV1I GCAGC 5 cut(s) 587, 796, 1027, 1114, 1424
BstX2I RGATCY 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 1168
BstYI RGATCY 1 cut(s) 36
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 7 cut(s) 5, 174, 320, 446, 945, 1026, 1329
BtgI CCRYGG 3 cut(s) 667, 678, 1087
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 4 cut(s) 483, 613, 1291, 1315
BtsIMutI CAGTG 3 cut(s) 315, 328, 859
Cac8I GCNNGC 4 cut(s) 193, 633, 690, 1327
CaiI CAGNNNCTG 2 cut(s) 224, 1222
CfoI GCGC 2 cut(s) 688, 967
Cfr10I RCCGGY 1 cut(s) 1325
Cfr13I GGNCC 3 cut(s) 148, 157, 675
Csp6I GTAC 5 cut(s) 343, 885, 1095, 1426, 1442
CviQI GTAC 5 cut(s) 343, 885, 1095, 1426, 1442
DdeI CTNAG 8 cut(s) 45, 197, 379, 489, 1008, 1132, 1182, 1416
DpnI GATC 4 cut(s) 38, 58, 1385, 1407
DpnII GATC 4 cut(s) 36, 56, 1383, 1405
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
EaeI YGGCCR 3 cut(s) 3, 172, 318
Eam1104I CTCTTC 2 cut(s) 242, 915
EarI CTCTTC 2 cut(s) 242, 915
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 4 cut(s) 667, 678, 861, 1087
Eco147I AGGCCT 1 cut(s) 1026
Eco24I GRGCYC 4 cut(s) 380, 521, 732, 910
Eco31I GGTCTC 2 cut(s) 172, 723
Eco32I GATATC 1 cut(s) 1152
Eco47I GGWCC 3 cut(s) 148, 157, 675
Eco53kI GAGCTC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 582
Eco81I CCTNAGG 1 cut(s) 489
Eco91I GGTNACC 1 cut(s) 1082
EcoICRI GAGCTC 1 cut(s) 378
EcoO65I GGTNACC 1 cut(s) 1082
EcoRII CCWGG 3 cut(s) 739, 796, 831
EcoRV GATATC 1 cut(s) 1152
EcoT14I CCWWGG 4 cut(s) 667, 678, 861, 1087
EcoT22I ATGCAT 1 cut(s) 1315
EcoT38I GRGCYC 4 cut(s) 380, 521, 732, 910
ErhI CCWWGG 4 cut(s) 667, 678, 861, 1087
FalI AAGNNNNNCTT 2 cut(s) 1048, 1080
FaqI GGGAC 2 cut(s) 304, 1274
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 1383
FblI GTMKAC 2 cut(s) 474, 1194
Fnu4HI GCNGC 6 cut(s) 22, 576, 810, 1041, 1128, 1413
FokI GGATG 4 cut(s) 470, 600, 1298, 1322
FriOI GRGCYC 4 cut(s) 380, 521, 732, 910
Fsp4HI GCNGC 6 cut(s) 22, 576, 810, 1041, 1128, 1413
FspBI CTAG 6 cut(s) 500, 699, 1004, 1016, 1334, 1370
GlaI GCGC 2 cut(s) 687, 966
GluI GCNGC 6 cut(s) 22, 576, 810, 1041, 1128, 1413
GsaI CCCAGC 2 cut(s) 906, 1226
GsuI CTGGAG 3 cut(s) 291, 762, 1041
HaeIII GGCC 7 cut(s) 5, 174, 320, 446, 945, 1026, 1329
HapII CCGG 2 cut(s) 175, 1326
HhaI GCGC 2 cut(s) 688, 967
Hin6I GCGC 2 cut(s) 686, 965
HinP1I GCGC 2 cut(s) 686, 965
HincII GTYRAC 1 cut(s) 1341
HindII GTYRAC 1 cut(s) 1341
HinfI GANTC 3 cut(s) 27, 744, 790
HpaI GTTAAC 1 cut(s) 1341
HpaII CCGG 2 cut(s) 175, 1326
HphI GGTGA 6 cut(s) 403, 422, 848, 1060, 1094, 1290
Hpy166II GTNNAC 4 cut(s) 475, 1109, 1195, 1341
Hpy188I TCNGA 4 cut(s) 48, 161, 603, 1218
Hpy188III TCNNGA 3 cut(s) 555, 617, 1169
Hpy8I GTNNAC 4 cut(s) 475, 1109, 1195, 1341
Hpy99I CGWCG 1 cut(s) 926
HpyAV CCTTC 4 cut(s) 92, 421, 833, 1340
HpyCH4III ACNGT 3 cut(s) 536, 854, 1175
HpyCH4IV ACGT 2 cut(s) 207, 471
HpyF10VI GCNNNNNNNGC 7 cut(s) 395, 572, 669, 736, 880, 971, 1133
HpyF3I CTNAG 8 cut(s) 45, 197, 379, 489, 1008, 1132, 1182, 1416
HpySE526I ACGT 2 cut(s) 207, 471
HspAI GCGC 2 cut(s) 686, 965
KroI GCCGGC 1 cut(s) 1325
KroNI GCCGGC 1 cut(s) 1327
Ksp22I TGATCA 1 cut(s) 1383
KspAI GTTAAC 1 cut(s) 1341
Kzo9I GATC 4 cut(s) 36, 56, 1383, 1405
LguI GCTCTTC 2 cut(s) 242, 915
LmnI GCTCC 2 cut(s) 1037, 1399
Lsp1109I GCAGC 5 cut(s) 587, 796, 1027, 1114, 1424
LweI GCATC 3 cut(s) 236, 610, 1300
MaeI CTAG 6 cut(s) 500, 699, 1004, 1016, 1334, 1370
MaeII ACGT 2 cut(s) 207, 471
MaeIII GTNAC 4 cut(s) 220, 745, 1048, 1082
MalI GATC 4 cut(s) 38, 58, 1385, 1407
MboI GATC 4 cut(s) 36, 56, 1383, 1405
MboII GAAGA 5 cut(s) 31, 259, 535, 785, 902
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 5 cut(s) 380, 521, 732, 910, 1111
MlsI TGGCCA 2 cut(s) 5, 320
MluNI TGGCCA 2 cut(s) 5, 320
MlyI GAGTC 1 cut(s) 753
MmeI TCCRAC 1 cut(s) 184
Mox20I TGGCCA 2 cut(s) 5, 320
Mph1103I ATGCAT 1 cut(s) 1315
MroNI GCCGGC 1 cut(s) 1325
MscI TGGCCA 2 cut(s) 5, 320
MseI TTAA 3 cut(s) 582, 1211, 1340
MslI CAYNNNNRTG 3 cut(s) 11, 411, 618
Msp20I TGGCCA 2 cut(s) 5, 320
MspA1I CMGCKG 1 cut(s) 1415
MspI CCGG 2 cut(s) 175, 1326
MspR9I CCNGG 3 cut(s) 741, 798, 833
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 3 cut(s) 741, 798, 833
MwoI GCNNNNNNNGC 7 cut(s) 395, 572, 669, 736, 880, 971, 1133
NaeI GCCGGC 1 cut(s) 1327
NcoI CCATGG 3 cut(s) 667, 678, 1087
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 36, 56, 1383, 1405
NgoMIV GCCGGC 1 cut(s) 1325
NlaIV GGNNCC 1 cut(s) 158
NmuCI GTSAC 3 cut(s) 745, 1048, 1082
NsiI ATGCAT 1 cut(s) 1315
NspI RCATGY 1 cut(s) 1317
NspV TTCGAA 1 cut(s) 245
PceI AGGCCT 1 cut(s) 1026
PciSI GCTCTTC 2 cut(s) 242, 915
PctI GAATGC 1 cut(s) 400
PdiI GCCGGC 1 cut(s) 1327
PfeI GAWTC 2 cut(s) 27, 790
PflFI GACNNNGTC 1 cut(s) 1175
PflMI CCANNNNNTGG 1 cut(s) 1019
PkrI GCNGC 6 cut(s) 23, 577, 811, 1042, 1129, 1414
PleI GAGTC 1 cut(s) 752
PpsI GAGTC 1 cut(s) 752
PsiI TTATAA 1 cut(s) 53
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 3 cut(s) 739, 796, 831
PspEI GGTNACC 1 cut(s) 1082
PspFI CCCAGC 2 cut(s) 902, 1222
PspGI CCWGG 3 cut(s) 739, 796, 831
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 3 cut(s) 148, 157, 675
PstNI CAGNNNCTG 2 cut(s) 224, 1222
PsuI RGATCY 1 cut(s) 36
PsyI GACNNNGTC 1 cut(s) 1175
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 5 cut(s) 344, 886, 1096, 1427, 1443
RsaNI GTAC 5 cut(s) 343, 885, 1095, 1426, 1442
RseI CAYNNNNRTG 3 cut(s) 11, 411, 618
SacI GAGCTC 1 cut(s) 380
SapI GCTCTTC 2 cut(s) 242, 915
SaqAI TTAA 3 cut(s) 582, 1211, 1340
SatI GCNGC 6 cut(s) 22, 576, 810, 1041, 1128, 1413
Sau3AI GATC 4 cut(s) 36, 56, 1383, 1405
Sau96I GGNCC 3 cut(s) 148, 157, 675
ScaI AGTACT 1 cut(s) 1096
SchI GAGTC 1 cut(s) 753
ScrFI CCNGG 3 cut(s) 741, 798, 833
SduI GDGCHC 5 cut(s) 380, 521, 732, 910, 1111
SfaNI GCATC 3 cut(s) 236, 610, 1300
SfuI TTCGAA 1 cut(s) 245
SinI GGWCC 3 cut(s) 148, 157, 675
SmiMI CAYNNNNRTG 3 cut(s) 11, 411, 618
SseBI AGGCCT 1 cut(s) 1026
SsiI CCGC 4 cut(s) 22, 124, 389, 812
SspMI CTAG 6 cut(s) 500, 699, 1004, 1016, 1334, 1370
SstI GAGCTC 1 cut(s) 380
StuI AGGCCT 1 cut(s) 1026
StyD4I CCNGG 3 cut(s) 739, 796, 831
StyI CCWWGG 4 cut(s) 667, 678, 861, 1087
TaaI ACNGT 3 cut(s) 536, 854, 1175
TaiI ACGT 2 cut(s) 210, 474
TaqI TCGA 2 cut(s) 245, 921
TatI WGTACW 2 cut(s) 1094, 1441
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 2 cut(s) 27, 790
Tru1I TTAA 3 cut(s) 582, 1211, 1340
Tru9I TTAA 3 cut(s) 582, 1211, 1340
TscAI CASTG 3 cut(s) 315, 328, 859
TseFI GTSAC 3 cut(s) 745, 1048, 1082
TseI GCWGC 5 cut(s) 575, 809, 1040, 1127, 1412
Tsp45I GTSAC 3 cut(s) 745, 1048, 1082
TspDTI ATGAA 2 cut(s) 585, 634
TspRI CASTG 3 cut(s) 315, 328, 859
Tth111I GACNNNGTC 1 cut(s) 1175
Van91I CCANNNNNTGG 1 cut(s) 1019
VneI GTGCAC 1 cut(s) 1107
VpaK11BI GGWCC 3 cut(s) 148, 157, 675
XapI RAATTY 2 cut(s) 584, 1456
XceI RCATGY 1 cut(s) 1317
XcmI CCANNNNNNNNNTGG 1 cut(s) 490
XmiI GTMKAC 2 cut(s) 474, 1194
XspI CTAG 6 cut(s) 500, 699, 1004, 1016, 1334, 1370
ZrmI AGTACT 1 cut(s) 1096
Zsp2I ATGCAT 1 cut(s) 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.