MD05G1241400.v1.1

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006452
Physical Location & Seq
Forward (+)
37186524 .. 37188900
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1241400.v1.1.491

Sequence Viewer

Length: 822 bp
ATGTTGCTGCTGCAGCCTTGGAGCGTTTCGAGCTCCACCGCGCTTCACAATTCTCTGGCTTTTTCCCACTCGGAAACCCAGCACAAAGCCATTTATAAACGACGCCGTTCAGCAGCACCCTTTTGCCTCAGAGCTCAGTTGCTGGACGGAATCGATCAGCTCGCGCATAACAAGGTTTTGATTGCGGCTGGAGTTTCAATGGCAGTTGGGCAGCTCACGAAGCCGGTGACTTCTGTGATTTTGTATGGGAAAGAGTTTGATTTCAAGGCGGTCGTTCAGGCTGGGGGATTCCCTTCTTCCCATTCCTCTGCAATGGTGGCTACTGCAACAACCCTTGGCCTCGAAAGGGGCCTTTCGGATTCAATATTTGGCCTTACTGTTGTTTATGCAGGCATTGTGATGTATGATGCTCAGGGGGTGCGAAGAGAAGTTGGCAATCATGCAAAAGTACTCAACAAAATGCTGCCCCTGAACACAACGGTGAACTCAGTCCCCACCCAGGACAGAGGTAGAATTAACTCTCAACCTCAAAAACCATTGTTGAAGTCGGAAAGCGTTGAGTCTCTGTTGTCTGAGGAAGCAAACATTCTCTCACCAAAAGCAACAAATGGCCCTTTAGCGTCTCAGTCCAAGGAGATAACAATGCAAGCTACTCAGACGCAGGTTTCTTCGGGCTTAGGAAGTAATGCCGAGGAAGGTTTAGAAAGAGCTGCCAATACTTCAACTCGGCTAAAAGAATCGATCGGCCATACTGAAGTAGAAGTCGCGGCCGGCGCTTTGTTGGGTTTCTTGGTAGGATTGGCGGTGTATGCGATAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

274

Amino Acids

28.97

Weight (kDa)

8.97

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 56 - 265 2.7e-37 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 652
AccII CGCG 3 cut(s) 41, 164, 767
AciI CCGC 5 cut(s) 39, 185, 269, 767, 803
AcoI YGGCCR 2 cut(s) 745, 768
AcuI CTGAAG 1 cut(s) 774
AcyI GRCGYC 1 cut(s) 103
AfaI GTAC 1 cut(s) 450
AfiI CCNNNNNNNGG 2 cut(s) 346, 499
AgsI TTSAA 5 cut(s) 198, 265, 363, 544, 723
AjnI CCWGG 1 cut(s) 498
AleI CACNNNNGTG 1 cut(s) 479
AluBI AGCT 6 cut(s) 33, 134, 160, 214, 650, 710
AluI AGCT 6 cut(s) 33, 134, 160, 214, 650, 710
Alw21I GWGCWC 2 cut(s) 35, 136
Alw26I GTCTC 2 cut(s) 567, 627
AlwNI CAGNNNCTG 1 cut(s) 142
AoxI GGCC 6 cut(s) 337, 349, 370, 610, 745, 768
ApeKI GCWGC 7 cut(s) 7, 10, 13, 113, 211, 463, 710
AspLEI GCGC 3 cut(s) 43, 166, 776
AspS9I GGNCC 2 cut(s) 349, 611
AsuHPI GGTGA 3 cut(s) 238, 493, 585
BanII GRGCYC 2 cut(s) 35, 136
Bbv12I GWGCWC 2 cut(s) 35, 136
BbvI GCAGC 5 cut(s) 25, 125, 223, 450, 697
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 500
BcoDI GTCTC 2 cut(s) 567, 627
BfmI CTRYAG 1 cut(s) 11
BfoI RGCGCY 1 cut(s) 777
BfuAI ACCTGC 1 cut(s) 652
BisI GCNGC 9 cut(s) 8, 11, 14, 114, 186, 212, 464, 711, 768
BlsI GCNGC 9 cut(s) 9, 12, 15, 115, 187, 213, 465, 712, 769
BmcAI AGTACT 1 cut(s) 450
Bme1390I CCNGG 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 349, 611
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 1 cut(s) 500
BmsI GCATC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 2 cut(s) 411, 676
Bsa29I ATCGAT 2 cut(s) 153, 740
BsaHI GRCGYC 1 cut(s) 103
BsaJI CCNNGG 5 cut(s) 17, 334, 498, 630, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 499
Bse118I RCCGGY 2 cut(s) 223, 770
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 500
BseCI ATCGAT 2 cut(s) 153, 740
BseDI CCNNGG 5 cut(s) 17, 334, 498, 630, 690
BseLI CCNNNNNNNGG 2 cut(s) 346, 499
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 7 cut(s) 142, 149, 425, 501, 564, 638, 668
BseX3I CGGCCG 1 cut(s) 768
BseXI GCAGC 5 cut(s) 25, 125, 223, 450, 697
BseYI CCCAGC 2 cut(s) 78, 281
Bsh1236I CGCG 3 cut(s) 41, 164, 767
Bsh1285I CGRYCG 3 cut(s) 273, 744, 771
BshFI GGCC 6 cut(s) 339, 351, 372, 612, 747, 770
BshVI ATCGAT 2 cut(s) 153, 740
BsiEI CGRYCG 3 cut(s) 273, 744, 771
BsiHKAI GWGCWC 2 cut(s) 35, 136
BsiSI CCGG 2 cut(s) 224, 771
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 2 cut(s) 346, 499
BsmAI GTCTC 2 cut(s) 567, 627
BsmBI CGTCTC 1 cut(s) 627
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 6 cut(s) 339, 351, 372, 612, 747, 770
Bsp1286I GDGCHC 2 cut(s) 35, 136
Bsp143I GATC 2 cut(s) 154, 741
BspACI CCGC 5 cut(s) 39, 185, 269, 767, 803
BspANI GGCC 6 cut(s) 339, 351, 372, 612, 747, 770
BspCNI CTCAG 7 cut(s) 141, 148, 424, 500, 565, 637, 667
BspDI ATCGAT 2 cut(s) 153, 740
BspFNI CGCG 3 cut(s) 41, 164, 767
BspLI GGNNCC 1 cut(s) 350
BspMAI CTGCAG 1 cut(s) 15
BspMI ACCTGC 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 2 cut(s) 223, 770
BssAI RCCGGY 2 cut(s) 223, 770
BssECI CCNNGG 5 cut(s) 17, 334, 498, 630, 690
BssMI GATC 2 cut(s) 154, 741
BssNI GRCGYC 1 cut(s) 103
BssT1I CCWWGG 3 cut(s) 17, 334, 630
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 379, 481
Bst6I CTCTTC 1 cut(s) 418
BstACI GRCGYC 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 162, 391, 648, 772
BstDEI CTNAG 8 cut(s) 128, 135, 411, 487, 573, 624, 654, 676
BstFNI CGCG 3 cut(s) 41, 164, 767
BstH2I RGCGCY 1 cut(s) 777
BstHHI GCGC 3 cut(s) 43, 166, 776
BstKTI GATC 2 cut(s) 157, 744
BstMAI GTCTC 2 cut(s) 567, 627
BstMBI GATC 2 cut(s) 154, 741
BstMCI CGRYCG 3 cut(s) 273, 744, 771
BstMWI GCNNNNNNNGC 6 cut(s) 13, 30, 220, 317, 773, 809
BstNI CCWGG 1 cut(s) 500
BstSCI CCNGG 1 cut(s) 498
BstSFI CTRYAG 1 cut(s) 11
BstUI CGCG 3 cut(s) 41, 164, 767
BstV1I GCAGC 5 cut(s) 25, 125, 223, 450, 697
BstZI CGGCCG 1 cut(s) 768
Bsu15I ATCGAT 2 cut(s) 153, 740
BsuRI GGCC 6 cut(s) 339, 351, 372, 612, 747, 770
BsuTUI ATCGAT 2 cut(s) 153, 740
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 4 cut(s) 162, 391, 648, 772
CaiI CAGNNNCTG 1 cut(s) 142
CfoI GCGC 3 cut(s) 43, 166, 776
Cfr10I RCCGGY 2 cut(s) 223, 770
Cfr13I GGNCC 2 cut(s) 349, 611
ClaI ATCGAT 2 cut(s) 153, 740
CseI GACGC 3 cut(s) 111, 609, 667
Csp6I GTAC 1 cut(s) 449
CviAII CATG 1 cut(s) 440
CviQI GTAC 1 cut(s) 449
DdeI CTNAG 8 cut(s) 128, 135, 411, 487, 573, 624, 654, 676
DpnI GATC 2 cut(s) 156, 743
DpnII GATC 2 cut(s) 154, 741
EaeI YGGCCR 2 cut(s) 745, 768
EagI CGGCCG 1 cut(s) 768
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
Ecl136II GAGCTC 2 cut(s) 33, 134
EclXI CGGCCG 1 cut(s) 768
Eco130I CCWWGG 3 cut(s) 17, 334, 630
Eco24I GRGCYC 2 cut(s) 35, 136
Eco52I CGGCCG 1 cut(s) 768
Eco53kI GAGCTC 2 cut(s) 33, 134
Eco57I CTGAAG 1 cut(s) 774
EcoICRI GAGCTC 2 cut(s) 33, 134
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 3 cut(s) 17, 334, 630
EcoT38I GRGCYC 2 cut(s) 35, 136
ErhI CCWWGG 3 cut(s) 17, 334, 630
Esp3I CGTCTC 1 cut(s) 627
FaeI CATG 1 cut(s) 443
FaiI YATR 8 cut(s) 96, 168, 246, 387, 405, 441, 750, 810
FaqI GGGAC 1 cut(s) 476
FatI CATG 1 cut(s) 439
Fnu4HI GCNGC 9 cut(s) 8, 11, 14, 114, 186, 212, 464, 711, 768
FriOI GRGCYC 2 cut(s) 35, 136
Fsp4HI GCNGC 9 cut(s) 8, 11, 14, 114, 186, 212, 464, 711, 768
GlaI GCGC 3 cut(s) 42, 165, 775
GluI GCNGC 9 cut(s) 8, 11, 14, 114, 186, 212, 464, 711, 768
GsaI CCCAGC 2 cut(s) 82, 285
GsuI CTGGAG 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 777
HaeIII GGCC 6 cut(s) 339, 351, 372, 612, 747, 770
HapII CCGG 2 cut(s) 224, 771
HgaI GACGC 3 cut(s) 111, 609, 667
HhaI GCGC 3 cut(s) 43, 166, 776
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 1 cut(s) 443
Hin6I GCGC 3 cut(s) 41, 164, 774
HinP1I GCGC 3 cut(s) 41, 164, 774
HinfI GANTC 5 cut(s) 150, 288, 359, 560, 737
HpaII CCGG 2 cut(s) 224, 771
HphI GGTGA 3 cut(s) 238, 493, 585
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 6 cut(s) 73, 131, 358, 550, 574, 657
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 2 cut(s) 303, 689
HpyCH4III ACNGT 2 cut(s) 379, 481
HpyCH4V TGCA 6 cut(s) 13, 311, 326, 389, 443, 646
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 30, 220, 317, 773, 809
HpyF3I CTNAG 8 cut(s) 128, 135, 411, 487, 573, 624, 654, 676
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 1 cut(s) 443
HspAI GCGC 3 cut(s) 41, 164, 774
KroI GCCGGC 1 cut(s) 770
KroNI GCCGGC 1 cut(s) 772
Kzo9I GATC 2 cut(s) 154, 741
LmnI GCTCC 2 cut(s) 21, 38
Lsp1109I GCAGC 5 cut(s) 25, 125, 223, 450, 697
LweI GCATC 1 cut(s) 397
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 156, 743
MboI GATC 2 cut(s) 154, 741
MboII GAAGA 3 cut(s) 288, 435, 660
MhlI GDGCHC 2 cut(s) 35, 136
MluCI AATT 2 cut(s) 49, 513
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 528
MnlI CCTC 7 cut(s) 137, 316, 350, 500, 537, 568, 685
MroNI GCCGGC 1 cut(s) 770
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 2 cut(s) 398, 479
MspI CCGG 2 cut(s) 224, 771
MspR9I CCNGG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 500
MvnI CGCG 3 cut(s) 41, 164, 767
MwoI GCNNNNNNNGC 6 cut(s) 13, 30, 220, 317, 773, 809
NaeI GCCGGC 1 cut(s) 772
NdeII GATC 2 cut(s) 154, 741
NgoMIV GCCGGC 1 cut(s) 770
NlaIII CATG 1 cut(s) 443
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 2 cut(s) 706, 715
NmuCI GTSAC 1 cut(s) 226
OliI CACNNNNGTG 1 cut(s) 479
PdiI GCCGGC 1 cut(s) 772
PfeI GAWTC 4 cut(s) 150, 288, 359, 737
PkrI GCNGC 9 cut(s) 9, 12, 15, 115, 187, 213, 465, 712, 769
Ple19I CGATCG 1 cut(s) 744
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PsiI TTATAA 1 cut(s) 96
Psp124BI GAGCTC 2 cut(s) 35, 136
Psp6I CCWGG 1 cut(s) 498
PspFI CCCAGC 2 cut(s) 78, 281
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 2 cut(s) 349, 611
PstI CTGCAG 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 142
PvuI CGATCG 1 cut(s) 744
RsaI GTAC 1 cut(s) 450
RsaNI GTAC 1 cut(s) 449
RseI CAYNNNNRTG 2 cut(s) 398, 479
SacI GAGCTC 2 cut(s) 35, 136
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 9 cut(s) 8, 11, 14, 114, 186, 212, 464, 711, 768
Sau3AI GATC 2 cut(s) 154, 741
Sau96I GGNCC 2 cut(s) 349, 611
ScaI AGTACT 1 cut(s) 450
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 1 cut(s) 500
SduI GDGCHC 2 cut(s) 35, 136
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 398, 479
Sse9I AATT 2 cut(s) 49, 513
SsiI CCGC 5 cut(s) 39, 185, 269, 767, 803
SspI AATATT 1 cut(s) 366
SstI GAGCTC 2 cut(s) 35, 136
StyD4I CCNGG 1 cut(s) 498
StyI CCWWGG 3 cut(s) 17, 334, 630
TaaI ACNGT 2 cut(s) 379, 481
TaqI TCGA 4 cut(s) 29, 153, 342, 740
TasI AATT 2 cut(s) 49, 513
TatI WGTACW 1 cut(s) 448
TauI GCSGC 2 cut(s) 188, 770
TfiI GAWTC 4 cut(s) 150, 288, 359, 737
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 7 cut(s) 7, 10, 13, 113, 211, 463, 710
Tsp45I GTSAC 1 cut(s) 226
TspGWI ACGGA 1 cut(s) 162
ZrmI AGTACT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.