Rw0G020830

Divergent PAP2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00956
OG0006452
Physical Location & Seq
Forward (+)
13586 .. 15723
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020830.1

Sequence Viewer

Length: 549 bp
ATGAAAAATTACGTTGACATCCATATCTTGCGATTTCTTCAGGTTCCATCGCTGATTTCCAGGTTTCTTCTACACCATGGTCCATGGGCATTATCTTTTAGTTTTGCCCATGGTGATTCGGTTTTGGTTGCAGCTGGTGTTTGTGAAGCAATTGGGAAGCTTTCCAAGCCTCTAACTAATGTAATTCTGTATGATAAAGAGTTTGATTTCAAGGCAACCGCTGGCGGCTTCCCTTCCACCCTATTTGGCTTAACTGTAGTTTATGCTAGTCTTGTATTGTATAATGCCCAGCTATCAATATATGGGGTGAGAAGAGAAGTAGGAAACCAAGCAAGAGTAGTGAACAAAACTTCGCCTCAAATTACAAGGCCTAAAACATCATCTTCGTTACAGTTGGATAGGCTCAAATCCCTTGCATTGGGGATGGAGAAGCAGGGTGTCATTGCCATCCACAGGGTTAAAGGGAAGAGTAAGCAGTTGGGAGTTGGTGGGACAGTGATGTTATCACTCGGAAGTTTAGTGAGAAGGAATCTCGACATCTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF212

Protein Analysis

182

Amino Acids

19.89

Weight (kDa)

10.15

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF212 PF02681 74 - 138 4e-06 Divergent PAP2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 219, 225
AcuI CTGAAG 1 cut(s) 23
AfiI CCNNNNNNNGG 2 cut(s) 418, 453
AgsI TTSAA 1 cut(s) 211
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 3 cut(s) 134, 160, 292
AluI AGCT 3 cut(s) 134, 160, 292
AoxI GGCC 1 cut(s) 368
ApeKI GCWGC 1 cut(s) 131
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 125, 319
AvaII GGWCC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 143
BccI CCATC 3 cut(s) 55, 418, 455
BciT130I CCWGG 1 cut(s) 61
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 255
BisI GCNGC 2 cut(s) 132, 226
BlsI GCNGC 2 cut(s) 133, 227
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 76, 83, 109
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 453
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 76, 83, 109
BseGI GGATG 3 cut(s) 18, 429, 447
BseLI CCNNNNNNNGG 2 cut(s) 418, 453
BseMI GCAATG 1 cut(s) 441
BseXI GCAGC 1 cut(s) 143
BseYI CCCAGC 1 cut(s) 288
BshFI GGCC 1 cut(s) 370
BslFI GGGAC 1 cut(s) 505
BslI CCNNNNNNNGG 2 cut(s) 418, 453
BsmFI GGGAC 1 cut(s) 505
BsnI GGCC 1 cut(s) 370
Bsp19I CCATGG 3 cut(s) 76, 83, 109
BspACI CCGC 2 cut(s) 219, 225
BspANI GGCC 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 441
BssECI CCNNGG 3 cut(s) 76, 83, 109
BssT1I CCWWGG 3 cut(s) 76, 83, 109
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 3 cut(s) 256, 393, 496
Bst6I CTCTTC 2 cut(s) 307, 461
BstC8I GCNNGC 1 cut(s) 223
BstDSI CCRYGG 3 cut(s) 76, 83, 109
BstF5I GGATG 3 cut(s) 18, 429, 447
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 255
BstV1I GCAGC 1 cut(s) 143
BsuRI GGCC 1 cut(s) 370
BtgI CCRYGG 3 cut(s) 76, 83, 109
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 18, 429, 447
BtsIMutI CAGTG 1 cut(s) 501
Cac8I GCNNGC 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 80
CspCI CAANNNNNGTGG 2 cut(s) 226, 261
CviAII CATG 3 cut(s) 77, 84, 110
CviJI RGCY 8 cut(s) 134, 160, 169, 228, 249, 292, 370, 403
CviKI_1 RGCY 8 cut(s) 134, 160, 169, 228, 249, 292, 370, 403
Eam1104I CTCTTC 2 cut(s) 307, 461
EarI CTCTTC 2 cut(s) 307, 461
Eco130I CCWWGG 3 cut(s) 76, 83, 109
Eco147I AGGCCT 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 3 cut(s) 76, 83, 109
ErhI CCWWGG 3 cut(s) 76, 83, 109
FaeI CATG 3 cut(s) 80, 87, 113
FaiI YATR 9 cut(s) 24, 78, 85, 111, 192, 264, 282, 301, 303
FaqI GGGAC 1 cut(s) 505
FatI CATG 3 cut(s) 76, 83, 109
Fnu4HI GCNGC 2 cut(s) 132, 226
FokI GGATG 3 cut(s) 5, 434, 436
Fsp4HI GCNGC 2 cut(s) 132, 226
FspBI CTAG 1 cut(s) 267
GluI GCNGC 2 cut(s) 132, 226
GsaI CCCAGC 1 cut(s) 292
HaeIII GGCC 1 cut(s) 370
Hin1II CATG 3 cut(s) 80, 87, 113
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 2 cut(s) 116, 529
HphI GGTGA 2 cut(s) 125, 319
Hpy166II GTNNAC 2 cut(s) 16, 343
Hpy188I TCNGA 1 cut(s) 512
Hpy188III TCNNGA 1 cut(s) 533
Hpy8I GTNNAC 2 cut(s) 16, 343
HpyAV CCTTC 2 cut(s) 243, 519
HpyCH4III ACNGT 3 cut(s) 256, 393, 496
HpyCH4IV ACGT 1 cut(s) 12
HpyCH4V TGCA 2 cut(s) 131, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 3 cut(s) 80, 87, 113
LpnPI CCDG 8 cut(s) 26, 46, 73, 120, 207, 302, 419, 439
Lsp1109I GCAGC 1 cut(s) 143
MaeI CTAG 1 cut(s) 267
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 1 cut(s) 387
MboII GAAGA 5 cut(s) 29, 59, 324, 375, 478
MfeI CAATTG 1 cut(s) 150
MluCI AATT 4 cut(s) 7, 150, 183, 360
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 2 cut(s) 180, 366
MseI TTAA 3 cut(s) 251, 459, 547
MspA1I CMGCKG 2 cut(s) 134, 221
MspR9I CCNGG 1 cut(s) 61
MunI CAATTG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 166
NcoI CCATGG 3 cut(s) 76, 83, 109
NlaIII CATG 3 cut(s) 80, 87, 113
NlaIV GGNNCC 1 cut(s) 45
PceI AGGCCT 1 cut(s) 370
PfeI GAWTC 2 cut(s) 116, 529
PkrI GCNGC 2 cut(s) 133, 227
Psp6I CCWGG 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 134
SaqAI TTAA 3 cut(s) 251, 459, 547
SatI GCNGC 2 cut(s) 132, 226
Sau96I GGNCC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 61
SetI ASST 6 cut(s) 15, 45, 65, 136, 162, 294
SfcI CTRYAG 1 cut(s) 255
SinI GGWCC 1 cut(s) 80
Sse9I AATT 4 cut(s) 7, 150, 183, 360
SseBI AGGCCT 1 cut(s) 370
SsiI CCGC 2 cut(s) 219, 225
SspMI CTAG 1 cut(s) 267
StuI AGGCCT 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 3 cut(s) 76, 83, 109
TaaI ACNGT 3 cut(s) 256, 393, 496
TaiI ACGT 1 cut(s) 15
TaqI TCGA 1 cut(s) 534
TasI AATT 4 cut(s) 7, 150, 183, 360
TauI GCSGC 1 cut(s) 228
TfiI GAWTC 2 cut(s) 116, 529
Tru1I TTAA 3 cut(s) 251, 459, 547
Tru9I TTAA 3 cut(s) 251, 459, 547
TscAI CASTG 1 cut(s) 501
TseI GCWGC 1 cut(s) 131
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 80
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.