MD05G1255100.v1.1

Transcription initiation factor TFIID subunit 8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006787
Physical Location & Seq
Reverse (-)
38553906 .. 38554793
888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1255100.v1.1.491

Sequence Viewer

Length: 888 bp
ATGTGCCAAAAACCCAAATCACGACGCAAAACCACAAAAATCGAACAAACCCCAACACTCCCAACCCAAAACCCGACCCCACCAGAGTTGCATTTCGCCATAGCCAGGATCGCGGTGTCTCAGATCAGCCAATCAGTCGGATTCAAATCAACGAAGCACTCCGCCCTCGAAACCCTGACCCACATCGCCGTCAAGTACATCGGCGCCGTAGCCTCGGCCTCGGCCTCCTACGCCGCCATGGCCAAGCGCACCAACACGAACGTCTTCGACTTCGCCAACGCCCTCCACGACTTGCAGTCGGTGCAGGGATTCAGAGGCGCCTCCGCCCTCCACGAAAACGGATTCTGCGTATTGGGTTCGAGCATTTTGAGCGAACTCGCCGGGTTCGTGAGGTGGAATCGGGAAATCCCCTTCGCCAGGCCGATTCCGCGGGTGCAGAAATTGGGCGCGGAGAAAAGGGGAAATGAGGTTCCCATTCCTCCGGAACTGGATTCCCGGAGGGGCTTGCACGTGCCGAGATGGCTGCCGGCGTTTCCGGACATGGGGAAGGCGGTTGGGAAGAGGAGGAATGGGGAGGAGCTGTGGGAGAATGTAGTGGCTAGTAATGGCGGCGGTGGCGGTGAAAGTGGAAATGGGAAAGGGAATTGCAGGGAATCGGTGGGAAAGAGTAAGAGGGGGAGAGTGAGGTTTAGGGTAGGAGGAGGGGAGGAGGAGAGTGGGGTGGTGGGGGGAATGGGTGTGGATTTGAGAAGTGGGGTGTGTAGAGGAGGGAAGAGGGTGTCTTGGAGTAACAGTAAAATTGGTTACACTAATTTCAGTACTAGAACAAGAATTCATGAATTTGATGATGTTGATGTTGATGATGATGATGATGGTGATAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
Bromo_TP TAF8_C

Protein Analysis

296

Amino Acids

32.0

Weight (kDa)

9.74

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromo_TP PF07524 29 - 98 2.3e-16 Bromodomain associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 203, 317
AccII CGCG 3 cut(s) 113, 430, 449
AccIII TCCGGA 2 cut(s) 481, 535
AclWI GGATC 1 cut(s) 116
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 2 cut(s) 831, 839
AcvI CACGTG 1 cut(s) 511
AcyI GRCGYC 2 cut(s) 204, 318
AfaI GTAC 2 cut(s) 197, 820
AfiI CCNNNNNNNGG 3 cut(s) 105, 417, 542
AgsI TTSAA 1 cut(s) 145
AhdI GACNNNNNGTC 1 cut(s) 295
AjnI CCWGG 2 cut(s) 104, 416
AloI GAACNNNNNNTCC 2 cut(s) 453, 485
AluBI AGCT 1 cut(s) 580
AluI AGCT 1 cut(s) 580
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 1 cut(s) 116
Aor13HI TCCGGA 2 cut(s) 481, 535
AoxI GGCC 4 cut(s) 216, 222, 240, 419
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 2 cut(s) 831, 839
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 4 cut(s) 206, 249, 320, 449
AsuC2I CCSGG 2 cut(s) 382, 496
AsuHPI GGTGA 2 cut(s) 632, 887
BalI TGGCCA 1 cut(s) 242
BanI GGYRCC 2 cut(s) 203, 317
BbrPI CACGTG 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 510
BccI CCATC 2 cut(s) 513, 866
BceAI ACGGC 2 cut(s) 173, 191
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 2 cut(s) 106, 418
BcnI CCSGG 2 cut(s) 382, 496
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 2 cut(s) 600, 822
BfoI RGCGCY 2 cut(s) 207, 321
BglI GCCNNNNNGGC 2 cut(s) 239, 520
BisI GCNGC 3 cut(s) 234, 524, 610
BlsI GCNGC 3 cut(s) 235, 525, 611
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 4 cut(s) 106, 382, 418, 496
BmeRI GACNNNNNGTC 1 cut(s) 295
BmiI GGNNCC 3 cut(s) 205, 319, 471
BmrFI CCNGG 4 cut(s) 106, 382, 418, 496
BpiI GAAGAC 1 cut(s) 256
BpuMI CCSGG 2 cut(s) 382, 496
BsaAI YACGTR 1 cut(s) 511
BsaBI GATNNNNATC 1 cut(s) 145
BsaHI GRCGYC 2 cut(s) 204, 318
BsaJI CCNNGG 4 cut(s) 213, 219, 237, 428
BsaWI WCCGGW 2 cut(s) 481, 535
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 778, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 417, 542
Bse118I RCCGGY 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 145
BseAI TCCGGA 2 cut(s) 481, 535
BseBI CCWGG 2 cut(s) 106, 418
BseDI CCNNGG 4 cut(s) 213, 219, 237, 428
BseJI GATNNNNATC 1 cut(s) 145
BseLI CCNNNNNNNGG 3 cut(s) 105, 417, 542
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 492
BseRI GAGGAG 6 cut(s) 577, 590, 714, 722, 725, 780
BseXI GCAGC 1 cut(s) 510
BsgI GTGCAG 2 cut(s) 323, 455
Bsh1236I CGCG 3 cut(s) 113, 430, 449
BshFI GGCC 4 cut(s) 218, 224, 242, 421
BshNI GGYRCC 2 cut(s) 203, 317
BsiSI CCGG 5 cut(s) 381, 482, 496, 527, 536
BslI CCNNNNNNNGG 3 cut(s) 105, 417, 542
BsmAI GTCTC 1 cut(s) 123
BsnI GGCC 4 cut(s) 218, 224, 242, 421
Bsp13I TCCGGA 2 cut(s) 481, 535
Bsp143I GATC 2 cut(s) 108, 123
Bsp19I CCATGG 1 cut(s) 237
BspANI GGCC 4 cut(s) 218, 224, 242, 421
BspCNI CTCAG 1 cut(s) 133
BspEI TCCGGA 2 cut(s) 481, 535
BspFNI CGCG 3 cut(s) 113, 430, 449
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 3 cut(s) 205, 319, 471
BspPI GGATC 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 203, 317
BsrFI RCCGGY 1 cut(s) 526
BsrI ACTGG 1 cut(s) 492
BssAI RCCGGY 1 cut(s) 526
BssECI CCNNGG 4 cut(s) 213, 219, 237, 428
BssMI GATC 2 cut(s) 108, 123
BssNI GRCGYC 2 cut(s) 204, 318
BssT1I CCWWGG 1 cut(s) 237
Bst2UI CCWGG 2 cut(s) 106, 418
Bst4CI ACNGT 1 cut(s) 794
Bst6I CTCTTC 2 cut(s) 554, 767
BstACI GRCGYC 2 cut(s) 204, 318
BstAPI GCANNNNNTGC 1 cut(s) 301
BstBAI YACGTR 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 506, 528
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 2 cut(s) 237, 428
BstENI CCTNNNNNAGG 1 cut(s) 415
BstFNI CGCG 3 cut(s) 113, 430, 449
BstH2I RGCGCY 2 cut(s) 207, 321
BstHHI GCGC 4 cut(s) 206, 249, 320, 449
BstKTI GATC 2 cut(s) 111, 126
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 108, 123
BstMWI GCNNNNNNNGC 8 cut(s) 110, 230, 239, 301, 369, 427, 520, 615
BstNI CCWGG 2 cut(s) 106, 418
BstSCI CCNGG 4 cut(s) 104, 380, 416, 494
BstUI CGCG 3 cut(s) 113, 430, 449
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 4 cut(s) 218, 224, 242, 421
BtgI CCRYGG 2 cut(s) 237, 428
BtgZI GCGATG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 506, 528
CciI TCATGA 1 cut(s) 835
CfoI GCGC 4 cut(s) 206, 249, 320, 449
Cfr10I RCCGGY 1 cut(s) 526
Cfr42I CCGCGG 1 cut(s) 431
CseI GACGC 1 cut(s) 33
Csp6I GTAC 2 cut(s) 196, 819
CspCI CAANNNNNGTGG 2 cut(s) 69, 104
CviAII CATG 3 cut(s) 238, 541, 836
CviQI GTAC 2 cut(s) 196, 819
DdeI CTNAG 1 cut(s) 120
DinI GGCGCC 2 cut(s) 205, 319
DpnI GATC 2 cut(s) 110, 125
DpnII GATC 2 cut(s) 108, 123
DriI GACNNNNNGTC 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 240
Eam1104I CTCTTC 2 cut(s) 554, 767
Eam1105I GACNNNNNGTC 1 cut(s) 295
EarI CTCTTC 2 cut(s) 554, 767
EciI GGCGGA 2 cut(s) 151, 313
Eco130I CCWWGG 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 511
EcoNI CCTNNNNNAGG 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 831
EcoRII CCWGG 2 cut(s) 104, 416
EcoT14I CCWWGG 1 cut(s) 237
EgeI GGCGCC 2 cut(s) 205, 319
EheI GGCGCC 2 cut(s) 205, 319
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 3 cut(s) 241, 544, 839
FaiI YATR 4 cut(s) 101, 239, 542, 837
FatI CATG 3 cut(s) 237, 540, 835
FauI CCCGC 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 234, 524, 610
Fsp4HI GCNGC 3 cut(s) 234, 524, 610
FspBI CTAG 2 cut(s) 600, 822
GlaI GCGC 4 cut(s) 205, 248, 319, 448
GluI GCNGC 3 cut(s) 234, 524, 610
HaeII RGCGCY 2 cut(s) 207, 321
HaeIII GGCC 4 cut(s) 218, 224, 242, 421
HapII CCGG 5 cut(s) 381, 482, 496, 527, 536
HgaI GACGC 1 cut(s) 33
HhaI GCGC 4 cut(s) 206, 249, 320, 449
Hin1I GRCGYC 2 cut(s) 204, 318
Hin1II CATG 3 cut(s) 241, 544, 839
Hin6I GCGC 4 cut(s) 204, 247, 318, 447
HinP1I GCGC 4 cut(s) 204, 247, 318, 447
HinfI GANTC 7 cut(s) 141, 309, 342, 397, 424, 491, 653
HpaII CCGG 5 cut(s) 381, 482, 496, 527, 536
HphI GGTGA 2 cut(s) 632, 887
Hpy188I TCNGA 3 cut(s) 123, 140, 314
Hpy188III TCNNGA 6 cut(s) 21, 388, 401, 482, 536, 836
Hpy99I CGWCG 1 cut(s) 27
HpyAV CCTTC 2 cut(s) 421, 541
HpyCH4III ACNGT 1 cut(s) 794
HpyCH4IV ACGT 2 cut(s) 261, 510
HpyCH4V TGCA 6 cut(s) 91, 295, 304, 436, 508, 648
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 230, 239, 301, 369, 427, 520, 615
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 2 cut(s) 261, 510
Hsp92I GRCGYC 2 cut(s) 204, 318
Hsp92II CATG 3 cut(s) 241, 544, 839
HspAI GCGC 4 cut(s) 204, 247, 318, 447
KasI GGCGCC 2 cut(s) 203, 317
Kpn2I TCCGGA 2 cut(s) 481, 535
KroI GCCGGC 1 cut(s) 526
KroNI GCCGGC 1 cut(s) 528
KspI CCGCGG 1 cut(s) 431
Kzo9I GATC 2 cut(s) 108, 123
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 1 cut(s) 510
MaeI CTAG 2 cut(s) 600, 822
MaeII ACGT 2 cut(s) 261, 510
MaeIII GTNAC 2 cut(s) 788, 803
MalI GATC 2 cut(s) 110, 125
MboI GATC 2 cut(s) 108, 123
MboII GAAGA 3 cut(s) 256, 571, 784
MlsI TGGCCA 1 cut(s) 242
MluCI AATT 6 cut(s) 440, 643, 798, 811, 831, 839
MluNI TGGCCA 1 cut(s) 242
Mly113I GGCGCC 2 cut(s) 204, 318
MmeI TCCRAC 1 cut(s) 118
Mox20I TGGCCA 1 cut(s) 242
MroI TCCGGA 2 cut(s) 481, 535
MroNI GCCGGC 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 5 cut(s) 381, 482, 496, 527, 536
MspR9I CCNGG 4 cut(s) 106, 382, 418, 496
MvaI CCWGG 2 cut(s) 106, 418
MvnI CGCG 3 cut(s) 113, 430, 449
MwoI GCNNNNNNNGC 8 cut(s) 110, 230, 239, 301, 369, 427, 520, 615
NaeI GCCGGC 1 cut(s) 528
NarI GGCGCC 2 cut(s) 204, 318
NciI CCSGG 2 cut(s) 382, 496
NcoI CCATGG 1 cut(s) 237
NdeII GATC 2 cut(s) 108, 123
NgoMIV GCCGGC 1 cut(s) 526
NlaIII CATG 3 cut(s) 241, 544, 839
NlaIV GGNNCC 3 cut(s) 205, 319, 471
NmeAIII GCCGAG 3 cut(s) 194, 200, 540
PagI TCATGA 1 cut(s) 835
PcsI WCGNNNNNNNCGW 3 cut(s) 285, 345, 384
PdiI GCCGGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 7 cut(s) 141, 309, 342, 397, 424, 491, 653
PfoI TCCNGGA 1 cut(s) 494
PkrI GCNGC 3 cut(s) 235, 525, 611
PluTI GGCGCC 2 cut(s) 207, 321
PmaCI CACGTG 1 cut(s) 511
PmlI CACGTG 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 104, 416
PspCI CACGTG 1 cut(s) 511
PspGI CCWGG 2 cut(s) 104, 416
PspN4I GGNNCC 3 cut(s) 205, 319, 471
RsaI GTAC 2 cut(s) 197, 820
RsaNI GTAC 2 cut(s) 196, 819
SacII CCGCGG 1 cut(s) 431
SatI GCNGC 3 cut(s) 234, 524, 610
Sau3AI GATC 2 cut(s) 108, 123
ScaI AGTACT 1 cut(s) 820
ScrFI CCNGG 4 cut(s) 106, 382, 418, 496
SetI ASST 6 cut(s) 264, 395, 471, 513, 582, 689
SfoI GGCGCC 2 cut(s) 205, 319
Sfr303I CCGCGG 1 cut(s) 431
SgrBI CCGCGG 1 cut(s) 431
Sse9I AATT 6 cut(s) 440, 643, 798, 811, 831, 839
SspDI GGCGCC 2 cut(s) 203, 317
SspMI CTAG 2 cut(s) 600, 822
StyD4I CCNGG 4 cut(s) 104, 380, 416, 494
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 1 cut(s) 794
TaiI ACGT 2 cut(s) 264, 513
TaqI TCGA 4 cut(s) 42, 168, 267, 359
TasI AATT 6 cut(s) 440, 643, 798, 811, 831, 839
TatI WGTACW 2 cut(s) 195, 818
TauI GCSGC 2 cut(s) 236, 612
TfiI GAWTC 7 cut(s) 141, 309, 342, 397, 424, 491, 653
TseI GCWGC 1 cut(s) 523
TspDTI ATGAA 2 cut(s) 824, 852
TspGWI ACGGA 1 cut(s) 354
XagI CCTNNNNNAGG 1 cut(s) 415
XapI RAATTY 2 cut(s) 831, 839
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 2 cut(s) 600, 822
ZrmI AGTACT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.