MD05G1291600.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007664
Physical Location & Seq
Forward (+)
42312892 .. 42317137
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1291600.v1.1.491

Sequence Viewer

Length: 3393 bp
ATGGAGCTTGAAAAAAGCAGTGACAGAACACGCGCCGCCGCATTCTCCTCCTCCTCCGCCACAAGCAACATGAAACCCACCCCCGCTGCTGCCAGACCCGACAAGTCAATCGCTCACTACAGGGAGGACGCCAACCTCTTGGCCGAGTACGAGCAGTCTGCCGTCTCCGGTAAGTCCTTCAACTACACAAGGTCAGTGCTTTTTCCTCCGGATTCTGTCCCCGAGGAGCAAGTCAGTGCCTACTTATCGAGAATCCAAAGGGGTTCTCTCGTCCAATCCTTTGGCTGCATGCTTGCAATTGAAGAACCTACTTTTCGAATTATCGGTTTTAGTGAGAATTGCCATGAATTGCTCGGTTTGGATAAATTGGATAGTGTTTTCGAGTCAAATGAGTTAATGGGTTTGATTGGGGTGGATTGTAGAACTCTGTTTACTCCTCCTTCCGCGGCTTCATTGACGAAAGCTGCTGCTTCGAGGGAGATTTCGCTGTTAAACCCGATTTGGGTGTATTCTAGGAGTACCCAGAAGCCATTTTATGCTATTTTGCATAGAATTGATGTGGGAATTGTGGTTGATTTGGAGCCTGCTAGGTCTGGTGATCCTGCATTGTCACTTGCAGGGGCCGTACAGTCACAGAAGCTCGCGGTTCGCGCAATTTCTAGGCTTCAGTCTCTCCCTGGAGGGGACCTTGGTGTGTTGTGTGACACAGTTGTAGAAGATGTTCGGAAGCTTACTGGATATGACAGAGTTATGGTTTATAAGTTCCATGACGATGATCACGGTGAGGTTGTGTCTGAAATTAGAAGGTCGGATTTGGAGTCTTATTTGGGTTTGCATTACCCTGCCACGGATATCCCTCAAGCTGCCCGTTTCTTGTTCAAGCAGAATCGCGTGCGTATGATCTGCGATTGCTATGCGAATCCAGTTAAAGTCATTCAAAGTGAAGAACTGAAGCAACCTCTGTGCTTGGTCAATTCAACTCTCCGGTCTCCACACGGCTGCCACAGACAGTACATGGCGAACATGGGCTCGATTGCCTCGTTGGTGATGGCGGTTATTATCAATGGTAATGATTCAACGAAGCTTTGGGGGTTGGTGGTGTGCCACCATACTTCCCCCCGCCATGTCCCTTTTCCTCTTCGCTACGCTTGCGAGTTTCTTATGCAGGCATTTGGGCTGCAAATCTACATGGAGCTTCAATTGGCATCGCAGTTGGCTGAGAAAAAGATTCTCAGAACACAAACCTTGCTTTGTGACATGCTCCTCCGTGATTCCCCATCCGGTATTGTGACCCAATCTCCGAGTATAATGAATCTCGTCAAGTGTGATGGGGCTGCATTGTACTATGGTGGGACATGCTGGTTGATGGGTGTAACTCCAACTGAATCACAGGTTAAAGATATAGCAGAGTGGCTGCTAAACAATCATGGGGATTCCACCGGTCTGAGTACCGATAGTTTGGCGGAAGCTGGTTACCCCGGTGCACCTTTACTGGGCAATGCAGTTTGTGGTATGGCTACCGCAAGGGTCAGTTCAAAAGATTTCTTGTTTTGGTTCAGGTCCCACACGGCAGAGGAAGTTAAATGGGGAGGAGCTAAGCATCATCCAGGGGACAAGGATGACGGTGAAAGGATGCACCCGAGATCATCGTTTAAAGCTTTTCTTGAGATAGTAAAAACTAGAAGTTTGCCTTGGGAGATCTCTGAGATTAATGCCATCCACTCTTTGCAGCTCATCATGAGAGACTCGTTTCAGGATATCGAGGAAACTGGTTCGAGGGCAGTGCAACAGAGTGACGCTGAGATGCAGTTGCAGGGGATGGATGAACTCAGTTCCGTGGCATGTGAAATGGTTAAGTTGATTGAGACAGCTTCTGTTCCAATTTTCGGGGTTGATTCGGATGGTCTCATCAATGGATGGAACGCAAAAATAGCTGAGTTGACAGGATTACAAGATAGCGAAGCGATGGGGAAGTCCCTAGTTAATGAGATTGTTTACGAGGACTCGCGTGAAGCTGTTGAAAATCTTTTACGCAGAGCCTTACAAGGTGAGGAGGACAAGAACGTTGAGTTAAAACTGAGAAATTTCGGGCTTTCGCAGCATAACTCGGTTGTCTATATTGTTGCCAGCACTTGCACAAGTAGGAGTCCTGCAAAGAATGTTGTCGGTGCATGCTTTGTGGGGCAGGATATTACTTGTGAAAAGGTTGTTATGGAAAAATTCATCCGCCTGCAAGGGGATTACAAGGCAATCATACAAAGTCTTAACCCGTTGATTCCACCGATATTTGCATCTGACGAGAATGCCTGCTGCTCCGAATGGAATTCAGCCATGGAAAAGCTTACTGGTTGGACAAGAGATAACGTTATTGGTAAAACGCTTCCTGGGGAAATCTTTGGAGGTCTTTGTCGACTGAGAGGTCAAGATATACTGACTAAATTCATGATTATGTTGTACCAAGGAATCAGTGGTAAAGAGATTGAGAAGTTTCCGTTCGGGTTTTTTGATAGAAAGGGGAAGTTCGTTGAGGCGCTCTTAACAGCTAACAAGAGGACTGATGCAGGTGGGAATATAATTGGTTGCTTCTGTTTCCTGCAAATTTTTTTGCCTGACTTGCAGTCGGCCTTGGAAGGACACATACAAGAGGATGGAGAATGCTTTTCGAAACTTAAGGAATTAGCATACATGCGACAAGAGATGAAAAACGCGTTAAGTGGTATTCAGTTCACCCACAGACTCCTTGAAAGTACGACTATTTCAGAATATCAGAAGCAATTCCTCGACACTAGCAATGCGTGCGAAAGACAAATCATGACAATCATCGAGGATATAGATGTGAAGAGCATAGCCGAAGGTAGCGTGAAGCTGAACATGGAAGAATTTATGTTGGGAAGCGTAGTGGACGCCATTATTAGCCAATCAATGCTCTTGCTGAGGGAAAAGAATCTGCAGCTCTTTCATGAGATTCCAGAAGAAATTAAGTCACTGTCTTTACATGGCGATCAAATCCGGCTTCAGTTGGTTCTGTCAGATTTCTTGCTCAATGTGGTGAATCACACACCTTCCTCGAATGGCTGGGTCGAAATTAAAATTTCACCTGGTTTAAAGTTGATACAGGACGGCAACAATTACATCCGCCTCCAGTTCAGAATGACACACCCCGGCCAAGGCCTTCCAACGACGCTTGTACAGGATATGTTCGAGGGAGGAAACAGATGGACGACACAGGAAGGTCTCGGACTAAATCTGTCACGAAAAATTCTGAACAGAATGAATGGTCAGGTGCAGTATGTTAGAGAGCACGATAAATGCTACTTCCTCGTTAACATTGAACTTAAACACAAGAAAGAAAGGCAGAGGGTATTTGTGTCTCATGAGAGCACAACGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1131

Amino Acids

125.94

Weight (kDa)

5.71

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 80 - 196 3.9e-33 PAS fold
GAF PF01590 229 - 397 7.1e-19 GAF domain
PHY PF00360 412 - 585 9.8e-57 Phytochrome region
PAS PF00989 616 - 731 2e-25 PAS fold
PAS_4 PF08448 624 - 732 3.6e-08 PAS fold
PAS PF00989 747 - 866 3.3e-18 PAS fold
HisKA PF00512 889 - 949 1.7e-08 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1000 - 1101 4e-12 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AarI CACCTGC 1 cut(s) 2552
AasI GACNNNNNNGTC 1 cut(s) 2418
Acc36I ACCTGC 1 cut(s) 2552
AccI GTMKAC 1 cut(s) 2410
AccII CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
AccIII TCCGGA 1 cut(s) 208
AclI AACGTT 2 cut(s) 2064, 2364
AclWI GGATC 1 cut(s) 593
AcoI YGGCCR 2 cut(s) 141, 3163
AcsI RAATTY 8 cut(s) 2083, 2219, 2323, 2438, 2598, 2879, 3090, 3258
AcuI CTGAAG 3 cut(s) 650, 971, 2999
AcyI GRCGYC 2 cut(s) 129, 2904
AfaI GTAC 9 cut(s) 149, 520, 627, 1013, 1343, 1450, 2456, 2749, 3189
AfiI CCNNNNNNNGG 7 cut(s) 502, 682, 847, 1493, 1886, 2236, 3167
AflII CTTAAG 1 cut(s) 2669
AflIII ACRYGT 1 cut(s) 2706
AgeI ACCGGT 1 cut(s) 1439
AhdI GACNNNNNGTC 1 cut(s) 2617
AjnI CCWGG 4 cut(s) 676, 1606, 2383, 3097
AjuI GAANNNNNNNTTGG 2 cut(s) 1675, 1707
Alw21I GWGCWC 3 cut(s) 1486, 3303, 3383
Alw26I GTCTC 8 cut(s) 169, 675, 993, 1737, 1859, 1910, 3239, 3375
Alw44I GTGCAC 1 cut(s) 1482
AlwI GGATC 1 cut(s) 593
AlwNI CAGNNNCTG 1 cut(s) 1874
Ama87I CYCGRG 2 cut(s) 221, 1639
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 5 cut(s) 141, 621, 2622, 3163, 3169
ApaLI GTGCAC 1 cut(s) 1482
ApoI RAATTY 8 cut(s) 2083, 2219, 2323, 2438, 2598, 2879, 3090, 3258
AseI ATTAAT 1 cut(s) 1710
AsiGI ACCGGT 1 cut(s) 1439
Asp700I GAANNNNTTC 2 cut(s) 720, 2774
AspLEI GCGC 3 cut(s) 35, 653, 2533
AspS9I GGNCC 3 cut(s) 621, 685, 1560
AsuC2I CCSGG 2 cut(s) 1479, 3162
AsuHPI GGTGA 8 cut(s) 608, 794, 1057, 1637, 2060, 2719, 3061, 3087
AsuII TTCGAA 2 cut(s) 316, 2663
AvaI CYCGRG 2 cut(s) 221, 1639
AvaII GGWCC 2 cut(s) 685, 1560
BaeGI GKGCMC 1 cut(s) 1486
BanII GRGCYC 1 cut(s) 1031
BarI GAAGNNNNNNTAC 2 cut(s) 2622, 2654
Bbv12I GWGCWC 3 cut(s) 1486, 3303, 3383
BbvCI CCTCAGC 1 cut(s) 2933
BceAI ACGGC 5 cut(s) 146, 608, 1012, 1584, 3136
BcgI CGANNNNNNTGC 6 cut(s) 140, 174, 896, 930, 1765, 1799
BciT130I CCWGG 4 cut(s) 678, 1608, 2385, 3099
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 2 cut(s) 1479, 3162
BcoDI GTCTC 8 cut(s) 169, 675, 993, 1737, 1859, 1910, 3239, 3375
BfaI CTAG 6 cut(s) 513, 588, 660, 1680, 1979, 2787
BfmI CTRYAG 2 cut(s) 118, 2948
BfoI RGCGCY 1 cut(s) 2534
BfrI CTTAAG 1 cut(s) 2669
BfuAI ACCTGC 1 cut(s) 2552
BglII AGATCT 1 cut(s) 1698
BlpI GCTNAGC 1 cut(s) 1596
Bme1390I CCNGG 6 cut(s) 678, 1479, 1608, 2385, 3099, 3162
Bme18I GGWCC 2 cut(s) 685, 1560
BmeRI GACNNNNNGTC 1 cut(s) 2617
BmeT110I CYCGRG 2 cut(s) 221, 1639
BmgT120I GGNCC 3 cut(s) 621, 685, 1560
BmiI GGNNCC 4 cut(s) 582, 622, 686, 1562
BmrFI CCNGG 6 cut(s) 678, 1479, 1608, 2385, 3099, 3162
BmrI ACTGGG 1 cut(s) 1502
BmsI GCATC 6 cut(s) 1214, 1609, 1623, 1794, 2300, 2548
BmuI ACTGGG 1 cut(s) 1502
BpmI CTGGAG 2 cut(s) 699, 3125
Bpu10I CCTNAGC 1 cut(s) 2933
Bpu1102I GCTNAGC 1 cut(s) 1596
Bpu14I TTCGAA 2 cut(s) 316, 2663
BpuEI CTTGAG 2 cut(s) 843, 1685
BpuMI CCSGG 2 cut(s) 1479, 3162
BsaHI GRCGYC 2 cut(s) 129, 2904
BsaI GGTCTC 3 cut(s) 993, 1910, 3239
BsaWI WCCGGW 5 cut(s) 167, 208, 984, 1280, 1439
BsaXI ACNNNNNCTCC 2 cut(s) 1282, 1312
Bsc4I CCNNNNNNNGG 7 cut(s) 502, 682, 847, 1493, 1886, 2236, 3167
Bse118I RCCGGY 1 cut(s) 1439
Bse1I ACTGG 6 cut(s) 739, 923, 1497, 1774, 2350, 3142
Bse3DI GCAATG 2 cut(s) 1504, 2797
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 4 cut(s) 678, 1608, 2385, 3099
BseLI CCNNNNNNNGG 7 cut(s) 502, 682, 847, 1493, 1886, 2236, 3167
BseMI GCAATG 2 cut(s) 1504, 2797
BseNI ACTGG 6 cut(s) 739, 923, 1497, 1774, 2350, 3142
BseRI GAGGAG 8 cut(s) 37, 40, 43, 239, 426, 1253, 1605, 2066
BseSI GKGCMC 1 cut(s) 1486
BseYI CCCAGC 1 cut(s) 3075
BsgI GTGCAG 1 cut(s) 3305
Bsh1236I CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
BshFI GGCC 5 cut(s) 143, 623, 2624, 3165, 3171
BshTI ACCGGT 1 cut(s) 1439
BsiHKAI GWGCWC 3 cut(s) 1486, 3303, 3383
BsiHKCI CYCGRG 2 cut(s) 221, 1639
BsiSI CCGG 8 cut(s) 168, 209, 985, 1281, 1440, 1479, 3010, 3162
BslFI GGGAC 7 cut(s) 203, 698, 1112, 1366, 1546, 1625, 1960
BslI CCNNNNNNNGG 7 cut(s) 502, 682, 847, 1493, 1886, 2236, 3167
BsmAI GTCTC 8 cut(s) 169, 675, 993, 1737, 1859, 1910, 3239, 3375
BsmBI CGTCTC 1 cut(s) 169
BsmFI GGGAC 7 cut(s) 203, 698, 1112, 1366, 1546, 1625, 1960
BsmI GAATGC 3 cut(s) 41, 2308, 2660
BsnI GGCC 5 cut(s) 143, 623, 2624, 3165, 3171
Bso31I GGTCTC 3 cut(s) 993, 1910, 3239
BsoBI CYCGRG 2 cut(s) 221, 1639
Bsp119I TTCGAA 2 cut(s) 316, 2663
Bsp1286I GDGCHC 4 cut(s) 1031, 1486, 3303, 3383
Bsp13I TCCGGA 1 cut(s) 208
Bsp1407I TGTACA 1 cut(s) 3187
Bsp143I GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
Bsp1720I GCTNAGC 1 cut(s) 1596
Bsp19I CCATGG 1 cut(s) 2331
BspANI GGCC 5 cut(s) 143, 623, 2624, 3165, 3171
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
BspHI TCATGA 5 cut(s) 1737, 2442, 2811, 2959, 3373
BspLI GGNNCC 4 cut(s) 582, 622, 686, 1562
BspMAI CTGCAG 1 cut(s) 2952
BspMI ACCTGC 1 cut(s) 2552
BspPI GGATC 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 2834
BspT104I TTCGAA 2 cut(s) 316, 2663
BspTI CTTAAG 1 cut(s) 2669
BspTNI GGTCTC 3 cut(s) 993, 1910, 3239
BsrDI GCAATG 2 cut(s) 1504, 2797
BsrFI RCCGGY 1 cut(s) 1439
BsrGI TGTACA 1 cut(s) 3187
BsrI ACTGG 6 cut(s) 739, 923, 1497, 1774, 2350, 3142
BssAI RCCGGY 1 cut(s) 1439
BssMI GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
BssNI GRCGYC 2 cut(s) 129, 2904
BssT1I CCWWGG 6 cut(s) 688, 1691, 2331, 2458, 2625, 3166
Bst2UI CCWGG 4 cut(s) 678, 1608, 2385, 3099
Bst4CI ACNGT 6 cut(s) 630, 709, 782, 1011, 1625, 2988
Bst6I CTCTTC 2 cut(s) 1143, 2834
BstACI GRCGYC 2 cut(s) 129, 2904
BstAFI CTTAAG 1 cut(s) 2669
BstAPI GCANNNNNTGC 1 cut(s) 2796
BstAUI TGTACA 1 cut(s) 3187
BstBI TTCGAA 2 cut(s) 316, 2663
BstDSI CCRYGG 4 cut(s) 444, 846, 1836, 2331
BstEII GGTNACC 1 cut(s) 1472
BstFNI CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
BstH2I RGCGCY 1 cut(s) 2534
BstHHI GCGC 3 cut(s) 35, 653, 2533
BstKTI GATC 6 cut(s) 601, 778, 903, 1646, 1701, 3004
BstMAI GTCTC 8 cut(s) 169, 675, 993, 1737, 1859, 1910, 3239, 3375
BstMBI GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
BstMWI GCNNNNNNNGC 6 cut(s) 650, 1149, 1931, 2098, 2614, 2796
BstNI CCWGG 4 cut(s) 678, 1608, 2385, 3099
BstNSI RCATGY 6 cut(s) 292, 1261, 1359, 1845, 2175, 2689
BstPI GGTNACC 1 cut(s) 1472
BstSCI CCNGG 6 cut(s) 676, 1477, 1606, 2383, 3097, 3160
BstSFI CTRYAG 2 cut(s) 118, 2948
BstSLI GKGCMC 1 cut(s) 1486
BstUI CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
BstX2I RGATCY 1 cut(s) 1698
BstXI CCANNNNNNTGG 2 cut(s) 139, 281
BstYI RGATCY 1 cut(s) 1698
BsuRI GGCC 5 cut(s) 143, 623, 2624, 3165, 3171
BtgI CCRYGG 4 cut(s) 444, 846, 1836, 2331
BtgZI GCGATG 2 cut(s) 1191, 1979
BtsI GCAGTG 2 cut(s) 25, 1788
BtsIMutI CAGTG 6 cut(s) 25, 201, 241, 1788, 2473, 2984
BveI ACCTGC 1 cut(s) 2552
CaiI CAGNNNCTG 1 cut(s) 1874
CciI TCATGA 5 cut(s) 1737, 2442, 2811, 2959, 3373
CfoI GCGC 3 cut(s) 35, 653, 2533
Cfr10I RCCGGY 1 cut(s) 1439
Cfr13I GGNCC 3 cut(s) 621, 685, 1560
Cfr42I CCGCGG 1 cut(s) 447
CseI GACGC 4 cut(s) 137, 1805, 2912, 3190
CsiI ACCWGGT 1 cut(s) 3097
Csp6I GTAC 9 cut(s) 148, 519, 626, 1012, 1342, 1449, 2455, 2748, 3188
CspAI ACCGGT 1 cut(s) 1439
CviQI GTAC 9 cut(s) 148, 519, 626, 1012, 1342, 1449, 2455, 2748, 3188
DpnI GATC 6 cut(s) 600, 777, 902, 1645, 1700, 3003
DpnII GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
DraI TTTAAA 2 cut(s) 1654, 3105
DrdI GACNNNNNNGTC 1 cut(s) 2418
DriI GACNNNNNGTC 1 cut(s) 2617
DseDI GACNNNNNNGTC 1 cut(s) 2418
EaeI YGGCCR 2 cut(s) 141, 3163
Eam1104I CTCTTC 2 cut(s) 1143, 2834
Eam1105I GACNNNNNGTC 1 cut(s) 2617
EarI CTCTTC 2 cut(s) 1143, 2834
EciI GGCGGA 4 cut(s) 46, 1478, 2216, 3125
Eco130I CCWWGG 6 cut(s) 688, 1691, 2331, 2458, 2625, 3166
Eco147I AGGCCT 1 cut(s) 3171
Eco24I GRGCYC 1 cut(s) 1031
Eco31I GGTCTC 3 cut(s) 993, 1910, 3239
Eco32I GATATC 2 cut(s) 853, 1759
Eco47I GGWCC 2 cut(s) 685, 1560
Eco57I CTGAAG 3 cut(s) 650, 971, 2999
Eco88I CYCGRG 2 cut(s) 221, 1639
Eco91I GGTNACC 1 cut(s) 1472
EcoO109I RGGNCCY 2 cut(s) 685, 1560
EcoO65I GGTNACC 1 cut(s) 1472
EcoRI GAATTC 1 cut(s) 2323
EcoRII CCWGG 4 cut(s) 676, 1606, 2383, 3097
EcoRV GATATC 2 cut(s) 853, 1759
EcoT14I CCWWGG 6 cut(s) 688, 1691, 2331, 2458, 2625, 3166
EcoT38I GRGCYC 1 cut(s) 1031
ErhI CCWWGG 6 cut(s) 688, 1691, 2331, 2458, 2625, 3166
Esp3I CGTCTC 1 cut(s) 169
FalI AAGNNNNNCTT 4 cut(s) 1647, 1679, 1675, 1707
FaqI GGGAC 7 cut(s) 203, 698, 1112, 1366, 1546, 1625, 1960
FauI CCCGC 2 cut(s) 91, 1127
FbaI TGATCA 1 cut(s) 775
FblI GTMKAC 1 cut(s) 2410
FriOI GRGCYC 1 cut(s) 1031
FspBI CTAG 6 cut(s) 513, 588, 660, 1680, 1979, 2787
GlaI GCGC 3 cut(s) 34, 652, 2532
GsaI CCCAGC 1 cut(s) 3079
GsuI CTGGAG 2 cut(s) 699, 3125
HaeII RGCGCY 1 cut(s) 2534
HaeIII GGCC 5 cut(s) 143, 623, 2624, 3165, 3171
HapII CCGG 8 cut(s) 168, 209, 985, 1281, 1440, 1479, 3010, 3162
HgaI GACGC 4 cut(s) 137, 1805, 2912, 3190
HhaI GCGC 3 cut(s) 35, 653, 2533
Hin1I GRCGYC 2 cut(s) 129, 2904
Hin6I GCGC 3 cut(s) 33, 651, 2531
HinP1I GCGC 3 cut(s) 33, 651, 2531
HincII GTYRAC 3 cut(s) 1941, 2411, 3325
HindII GTYRAC 3 cut(s) 1941, 2411, 3325
HindIII AAGCTT 4 cut(s) 728, 1082, 1656, 2339
HpaI GTTAAC 1 cut(s) 3325
HpaII CCGG 8 cut(s) 168, 209, 985, 1281, 1440, 1479, 3010, 3162
HphI GGTGA 8 cut(s) 608, 794, 1057, 1637, 2060, 2719, 3061, 3087
Hpy166II GTNNAC 8 cut(s) 432, 1484, 1941, 1996, 2411, 2727, 2902, 3325
Hpy8I GTNNAC 8 cut(s) 432, 1484, 1941, 1996, 2411, 2727, 2902, 3325
Hpy99I CGWCG 1 cut(s) 3184
HpyAV CCTTC 8 cut(s) 187, 450, 798, 2624, 2846, 3072, 3182, 3224
HpyCH4III ACNGT 6 cut(s) 630, 709, 782, 1011, 1625, 2988
HpyCH4IV ACGT 2 cut(s) 2064, 2364
HpyF10VI GCNNNNNNNGC 6 cut(s) 650, 1149, 1931, 2098, 2614, 2796
HpySE526I ACGT 2 cut(s) 2064, 2364
Hsp92I GRCGYC 2 cut(s) 129, 2904
HspAI GCGC 3 cut(s) 33, 651, 2531
Kpn2I TCCGGA 1 cut(s) 208
Ksp22I TGATCA 1 cut(s) 775
KspAI GTTAAC 1 cut(s) 3325
KspI CCGCGG 1 cut(s) 447
Kzo9I GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
LguI GCTCTTC 1 cut(s) 2834
LmnI GCTCC 7 cut(s) 4, 226, 580, 1192, 1266, 1592, 2318
LweI GCATC 6 cut(s) 1214, 1609, 1623, 1794, 2300, 2548
MabI ACCWGGT 1 cut(s) 3097
MaeI CTAG 6 cut(s) 513, 588, 660, 1680, 1979, 2787
MaeII ACGT 2 cut(s) 2064, 2364
MalI GATC 6 cut(s) 600, 777, 902, 1645, 1700, 3003
MboI GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
MboII GAAGA 7 cut(s) 314, 728, 956, 1130, 2851, 2888, 2984
MfeI CAATTG 2 cut(s) 297, 1199
MflI RGATCY 1 cut(s) 1698
MhlI GDGCHC 4 cut(s) 1031, 1486, 3303, 3383
MluI ACGCGT 1 cut(s) 2706
MlyI GAGTC 6 cut(s) 392, 827, 1739, 1997, 2155, 2730
MmeI TCCRAC 4 cut(s) 789, 1403, 2330, 3200
MroI TCCGGA 1 cut(s) 208
MroXI GAANNNNTTC 2 cut(s) 720, 2774
MslI CAYNNNNRTG 2 cut(s) 771, 2447
MspA1I CMGCKG 2 cut(s) 86, 446
MspCI CTTAAG 1 cut(s) 2669
MspI CCGG 8 cut(s) 168, 209, 985, 1281, 1440, 1479, 3010, 3162
MspR9I CCNGG 6 cut(s) 678, 1479, 1608, 2385, 3099, 3162
MunI CAATTG 2 cut(s) 297, 1199
Mva1269I GAATGC 3 cut(s) 41, 2308, 2660
MvaI CCWGG 4 cut(s) 678, 1608, 2385, 3099
MvnI CGCG 7 cut(s) 33, 446, 644, 651, 891, 2008, 2708
MwoI GCNNNNNNNGC 6 cut(s) 650, 1149, 1931, 2098, 2614, 2796
NciI CCSGG 2 cut(s) 1479, 3162
NcoI CCATGG 1 cut(s) 2331
NdeII GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
NlaIV GGNNCC 4 cut(s) 582, 622, 686, 1562
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 9 cut(s) 20, 609, 630, 701, 1253, 1288, 1793, 2982, 3249
NspI RCATGY 6 cut(s) 292, 1261, 1359, 1845, 2175, 2689
NspV TTCGAA 2 cut(s) 316, 2663
PaeI GCATGC 2 cut(s) 292, 2175
PagI TCATGA 5 cut(s) 1737, 2442, 2811, 2959, 3373
PaqCI CACCTGC 1 cut(s) 2552
PceI AGGCCT 1 cut(s) 3171
PciSI GCTCTTC 1 cut(s) 2834
PcsI WCGNNNNNNNCGW 1 cut(s) 1037
PctI GAATGC 3 cut(s) 41, 2308, 2660
PdmI GAANNNNTTC 2 cut(s) 720, 2774
PinAI ACCGGT 1 cut(s) 1439
PleI GAGTC 6 cut(s) 391, 826, 1739, 1997, 2154, 2730
PpsI GAGTC 6 cut(s) 391, 826, 1739, 1997, 2154, 2730
PpuMI RGGWCCY 2 cut(s) 685, 1560
PshBI ATTAAT 1 cut(s) 1710
PsiI TTATAA 1 cut(s) 759
Psp1406I AACGTT 2 cut(s) 2064, 2364
Psp5II RGGWCCY 2 cut(s) 685, 1560
Psp6I CCWGG 4 cut(s) 676, 1606, 2383, 3097
PspEI GGTNACC 1 cut(s) 1472
PspFI CCCAGC 1 cut(s) 3075
PspGI CCWGG 4 cut(s) 676, 1606, 2383, 3097
PspN4I GGNNCC 4 cut(s) 582, 622, 686, 1562
PspPI GGNCC 3 cut(s) 621, 685, 1560
PspPPI RGGWCCY 2 cut(s) 685, 1560
PsrI GAACNNNNNNTAC 4 cut(s) 415, 447, 705, 737
PstI CTGCAG 1 cut(s) 2952
PstNI CAGNNNCTG 1 cut(s) 1874
PsuI RGATCY 1 cut(s) 1698
RsaI GTAC 9 cut(s) 149, 520, 627, 1013, 1343, 1450, 2456, 2749, 3189
RsaNI GTAC 9 cut(s) 148, 519, 626, 1012, 1342, 1449, 2455, 2748, 3188
RseI CAYNNNNRTG 2 cut(s) 771, 2447
SacII CCGCGG 1 cut(s) 447
SalI GTCGAC 1 cut(s) 2409
SapI GCTCTTC 1 cut(s) 2834
Sau3AI GATC 6 cut(s) 598, 775, 900, 1643, 1698, 3001
Sau96I GGNCC 3 cut(s) 621, 685, 1560
SchI GAGTC 6 cut(s) 392, 827, 1739, 1997, 2155, 2730
ScrFI CCNGG 6 cut(s) 678, 1479, 1608, 2385, 3099, 3162
SduI GDGCHC 4 cut(s) 1031, 1486, 3303, 3383
SexAI ACCWGGT 1 cut(s) 3097
SfaNI GCATC 6 cut(s) 1214, 1609, 1623, 1794, 2300, 2548
SfcI CTRYAG 2 cut(s) 118, 2948
Sfr303I CCGCGG 1 cut(s) 447
SfuI TTCGAA 2 cut(s) 316, 2663
SgrBI CCGCGG 1 cut(s) 447
SinI GGWCC 2 cut(s) 685, 1560
SmiMI CAYNNNNRTG 2 cut(s) 771, 2447
SmlI CTYRAG 3 cut(s) 858, 1664, 2669
SmoI CTYRAG 3 cut(s) 858, 1664, 2669
SphI GCATGC 2 cut(s) 292, 2175
SseBI AGGCCT 1 cut(s) 3171
SspMI CTAG 6 cut(s) 513, 588, 660, 1680, 1979, 2787
StuI AGGCCT 1 cut(s) 3171
StyD4I CCNGG 6 cut(s) 676, 1477, 1606, 2383, 3097, 3160
StyI CCWWGG 6 cut(s) 688, 1691, 2331, 2458, 2625, 3166
TaaI ACNGT 6 cut(s) 630, 709, 782, 1011, 1625, 2988
TaiI ACGT 2 cut(s) 2067, 2367
TatI WGTACW 3 cut(s) 1011, 1341, 3187
TauI GCSGC 3 cut(s) 38, 41, 449
TscAI CASTG 6 cut(s) 25, 201, 241, 1788, 2473, 2991
TseFI GTSAC 9 cut(s) 20, 609, 630, 701, 1253, 1288, 1793, 2982, 3249
Tsp45I GTSAC 9 cut(s) 20, 609, 630, 701, 1253, 1288, 1793, 2982, 3249
TspGWI ACGGA 4 cut(s) 863, 1256, 1825, 2481
TspRI CASTG 6 cut(s) 25, 201, 241, 1788, 2473, 2991
Vha464I CTTAAG 1 cut(s) 2669
VneI GTGCAC 1 cut(s) 1482
VpaK11BI GGWCC 2 cut(s) 685, 1560
VspI ATTAAT 1 cut(s) 1710
XapI RAATTY 8 cut(s) 2083, 2219, 2323, 2438, 2598, 2879, 3090, 3258
XceI RCATGY 6 cut(s) 292, 1261, 1359, 1845, 2175, 2689
XcmI CCANNNNNNNNNTGG 1 cut(s) 2465
XmiI GTMKAC 1 cut(s) 2410
XmnI GAANNNNTTC 2 cut(s) 720, 2774
XspI CTAG 6 cut(s) 513, 588, 660, 1680, 1979, 2787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.