Rroxscaffold_1G00063660

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007664
Physical Location & Seq
Forward (+)
85462067 .. 85466826
4760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063660.1

Sequence Viewer

Length: 3387 bp
ATGGAGCCTGAGAGAACCACCGCTACATTCTCATCCTCAGCTACAAGCAACGTGAGGCCCAACAACAACACCATGATCACCGTCAAAAGAGATAAAGCAATTATTGCTCACTACAATGCAGACGCTGGACTTTTAAATGAGTTTGAACAGTCCACTGTGTCTGGTAAGTCCTTTAACTATACAAGATCAATGCTTCATCCACTTCAGTCTGTCCCTGAGGAGCACATAACTGCTTATTTCTCAAAAATCCAAAGAGGTGCTCTTGTTCAGTCTTTTGGGTGCATGCTTGCAATTGATGAACCTACGTTTAGGATTATCTGTTATAGTGAGAACTGCTCTGAGTTTTTGGGTTTGGATGGTACTTTTGAGTCAAAAGAAGGAAAGGGTCTGATTGGAATTGATGCTAAAACTCTGTTTACACCCTCATCTGGTGCTTCACTGGCCAAAGCGGCCGCTTCGAGGGAGCTATCATTGTTGAACCCAATTTGGGTGTATTCTAGGACTACCCAAAAGCCATTTTATGCTATATTGCATAGGATTGATGTTGGGATTGTGATTGATTTAGAGCCTGCCAAGTCTGGTGATCCTGCATTGTCCCTCGCAGGGGCTGTGCAGTCCCAGAAGCTTGCGGTTCGGGCAATCTCGAGGCTTCAGTCTCTCCCTGGAGGAGATATTGGGACTCTGTGTGATACTGTTGCGGAGGATGTTCAGAAGCTGACTGGATATGACAGAGTCATGGTGTACAAGTTTCATGAAGATGATCATGGGGAAATTGTGTCTGAGATCAGGAGGTCGGATTTGGAGTCCTATTTGGGTTTGCATTATCCTGCCACGGATATCCCTCAGGCGGCTCGGTTCTTGTTCAAGCAGAATCGGGTGCGGATGATTTGTGATTGCAATGCAAATCCTGTTAAACTCATTCAAAGTGAAGAGCTGGAGCAGCCTCTGTGCTTGGTGAATTCAACTCTCCGGTCACCACATGATTGCCACAGAACATACATGTCCAACATGGGCTCAGTTGCATCACTGGTCATGGCGGTTGCTATCAATAGTCCTGACTCCACCAAGCTTTGGGGGTTGGTGGTGTGCCATCACACTTCACCCCGCTATGTTCCTTTCCCTCTTCGCTATGCTTGTGAATTCCTTATGCAGGCATTTGTGTTGCAGCTTAACATGGAGCTTCAGTTGGCATTACAATTGGCCGAAAAGAAGATTCTCAGAACACAGACTTTACTTTGTGACATGCTCCTCCGGGATGCCCCGTCTGCTATCGTAACCCAATCTCCTAGTATAATGGATCTTGTCAAGTCTGATGGGGCTGCATTGTACTATAGTGGGACATGTTGGTTGACGGGTGTCACACCAACTGAGTCACAGGTTAAAGATATAGCCGAGTGGCTGCTGAAGAATCATGGGGATTCTACAGGACTGAGTACTGACAGTTTGGCTGAAGCTGGTTACCCTGCTGCAGCTCTACTAGGCAATGCGGTTTGTGGTATGGCTACTGCAAGAATCAGTTCAAAGGATTTTCTGTTTTGGTTCAGGTCTCACACCGCCGAGGAAGTCAAGTGGGGAGGAGCTAAGCATCATCCACAGGATAAGGATGATGGTGGAAGAATGCACCCAAGATCATCATTTAAGGCTTTTCTTGAAGTAGCGAAAACTAGAAGCTTGCCTTGGGAGGACTCCGAGATTAATGCCATCCACTCTTTACAGCTCATAATGAGAGACTCGTTCCAGGATATGGAGGAAACTGGTCCTAAGCCAACAAATAATGCTTGTCCTAGTGATGCTGAGATGCAGGTGGAGGGGATGGATGAGATCAGTTCTGTGGCATGTGAAATGGTTAAATTGATTGAGACGGCTCAAGTTCCGATTTTCGGGGTTGATTCAGAGGGTCTCATCAATGGATGGAATGCAAAGATGGCTGAATTGACAGGATTACAAGATGGTGAAGCAATGGGGAAGTCCCTTGTTAATGATATTGTTCATGAGGACTCGCGTGGAGCTGTTGAAAGTATCTTATCCAGAGCTTTACAAGGTGATGAGGACAAGAATATTGAGTTGAAACTGAGAAATTTTGGACTTGCTCAGCAAAACTCAGTGGTATATATTGTGGCCAACAGTTGCACAAGTAGGAATCATGCAAAGAATGTTGTTGGCGCATGCTTCGTGGGTCAAGACATTACTTGTGAAAAACTAGTTACGGATAAATTTATCCAGTTGCAAGGGGACTATAAGGCTATCATACAGAGTCTTAACCCATTGATTCCACCAATATTTGCATCTGATGAGAATGCCTACTGCTCTGAATGGAATGCAGCAATGGAAAAGCTGACTGGTTTGACAAGAGATGATGTTATTGGTAAAATGCTCCCTGGAGAAATCTTTGGAGAGTTCTGTCAATTGAAAGGTCAAGATTCGCTAACTAAATTTAGGATTGTACTATATCAAGGAATCATTGGGCAAGACATTGAAAAGTTTCCATTTGGGTTTTTTGATAGAGAAGGGAAGTATGTTGAGGTGCTCTTAACAGCAAGCAAGAGGACTGATGCCAATGGGAATATAATTGGTTGCTTCTGCTTCTTGCAAACATTTCTACCAGACCTGCAGCCAGCCATGGAAGGACTCGGACAAGAGGATAAATTCTCTTCAAAACTTAAGGAATTAACTTACATGCGACAAGAGATGAAAAATCCTTTAAGTGGTATCCGGTTTACACATAGACTCCTTGAAAATACAAATGTTTCAGAATATCAGAAGCAATTTCTTGACACTAATGATGCATGTGAAAGACAAATCATGGCAATCCTTGAGAATCTGGATACGAGGAGCATAGAGGAAGGCAGCAATGTGAAGCTGAACATGGAAGAATTTCTCTTGAGAACTCTTATAGATGCCATTGTGAGTCAATCAATGATCTCATTGAGGGAAAGAAATCTACAGTTATTTCATGAGATTCCAGAAGAACTCAAAACACTATCTTTGCTTGGCGACCAAATCAGGCTTCAGTTGGTCTTGTCAGATTTCTTGCTTAATGTGGTGCATCATACACCTTCTCCAGATGGCTGGGTTGAAATCAGAATTTCACCAGGTCTAAAGCGAATACATGATGGCAACACCTATATCCGTTTACAGTTCAGAATGACTCACCCTGGTCAAGGAATTCCCACTGCACTTATTCAAGATATGTTCGCCGGTGAAAACCAGTGGAATACACAAGAAGGACTTGGGCTAAACCTGTCCAGAAAACTTCTTGGTAGAATGAATGGTCAGGTCCAGTATGTAAGAGAGCATGATAAGTGTTACTTCCTCATTGATATTGAACTTAGAACACGGAAAGAAAGGCAGAGGGTGTTACTGTCACAATCTGATAGGAGCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1128

Amino Acids

125.88

Weight (kDa)

5.47

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 79 - 191 1.7e-33 PAS fold
GAF PF01590 224 - 394 5.1e-17 GAF domain
PHY PF00360 407 - 582 7.1e-57 Phytochrome region
PAS PF00989 614 - 729 2.1e-24 PAS fold
PAS_4 PF08448 621 - 730 9.6e-07 PAS fold
PAS PF00989 745 - 864 5e-18 PAS fold
HisKA PF00512 886 - 948 1.5e-09 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 998 - 1099 1.7e-11 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1792
Acc36I ACCTGC 2 cut(s) 1792, 2616
AccB7I CCANNNNNTGG 2 cut(s) 1071, 1744
AccII CGCG 1 cut(s) 2002
AclWI GGATC 2 cut(s) 578, 1305
AcoI YGGCCR 4 cut(s) 441, 450, 1200, 2118
AcsI RAATTY 9 cut(s) 958, 1139, 2077, 2213, 2432, 2645, 2873, 3084, 3165
AcuI CTGAAG 6 cut(s) 188, 635, 1166, 1424, 1470, 2993
AfaI GTAC 5 cut(s) 361, 743, 1328, 1435, 2445
AflII CTTAAG 1 cut(s) 2660
AflIII ACRYGT 2 cut(s) 999, 1340
AhlI ACTAGT 1 cut(s) 2200
AjnI CCWGG 5 cut(s) 661, 1737, 2377, 3091, 3154
AjuI GAANNNNNNNTTGG 4 cut(s) 1660, 1692, 2991, 3023
Alw21I GWGCWC 4 cut(s) 225, 262, 2529, 3383
Alw26I GTCTC 5 cut(s) 660, 1551, 1722, 1853, 1904
AlwI GGATC 2 cut(s) 578, 1305
AlwNI CAGNNNCTG 4 cut(s) 125, 608, 715, 946
Ama87I CYCGRG 1 cut(s) 643
AoxI GGCC 5 cut(s) 56, 441, 450, 1200, 2118
ApeKI GCWGC 9 cut(s) 940, 1165, 1319, 1399, 1466, 1469, 2321, 2611, 2847
ApoI RAATTY 9 cut(s) 958, 1139, 2077, 2213, 2432, 2645, 2873, 3084, 3165
AseI ATTAAT 1 cut(s) 1695
Asp700I GAANNNNTTC 3 cut(s) 1516, 2481, 2874
AspLEI GCGC 1 cut(s) 2165
AspS9I GGNCC 3 cut(s) 57, 1757, 3277
AsuC2I CCSGG 1 cut(s) 1253
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 2 cut(s) 1757, 3277
AxyI CCTNAGG 2 cut(s) 216, 843
BalI TGGCCA 2 cut(s) 443, 2120
BanII GRGCYC 1 cut(s) 1016
Bbv12I GWGCWC 4 cut(s) 225, 262, 2529, 3383
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 9 cut(s) 952, 1177, 1306, 1386, 1453, 1481, 2333, 2623, 2859
BceAI ACGGC 1 cut(s) 1878
BcgI CGANNNNNNTGC 2 cut(s) 1679, 1713
BciT130I CCWGG 5 cut(s) 663, 1739, 2379, 3093, 3156
BciVI GTATCC 2 cut(s) 2720, 2818
BclI TGATCA 2 cut(s) 75, 760
BcnI CCSGG 1 cut(s) 1253
BcoDI GTCTC 5 cut(s) 660, 1551, 1722, 1853, 1904
BcuI ACTAGT 1 cut(s) 2200
BfaI CTAG 6 cut(s) 498, 1287, 1478, 1665, 1785, 2201
BfmI CTRYAG 5 cut(s) 1330, 1422, 1467, 2609, 2942
BfrI CTTAAG 1 cut(s) 2660
BfuAI ACCTGC 2 cut(s) 1792, 2616
BfuI GTATCC 2 cut(s) 2720, 2818
BglI GCCNNNNNGGC 1 cut(s) 449
BlpI GCTNAGC 2 cut(s) 1581, 2091
BmcAI AGTACT 1 cut(s) 1435
Bme1390I CCNGG 6 cut(s) 663, 1253, 1739, 2379, 3093, 3156
Bme18I GGWCC 2 cut(s) 1757, 3277
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 3 cut(s) 57, 1757, 3277
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 6 cut(s) 663, 1253, 1739, 2379, 3093, 3156
BoxI GACNNNNGTC 1 cut(s) 1355
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpmI CTGGAG 4 cut(s) 684, 956, 2400, 3045
Bpu10I CCTNAGC 2 cut(s) 37, 1761
Bpu1102I GCTNAGC 2 cut(s) 1581, 2091
BpuEI CTTGAG 3 cut(s) 1851, 2834, 2902
BpuMI CCSGG 1 cut(s) 1253
BsaAI YACGTR 1 cut(s) 3384
BsaI GGTCTC 2 cut(s) 1551, 1904
BsaJI CCNNGG 7 cut(s) 661, 831, 1557, 1676, 2377, 2619, 3154
BsaWI WCCGGW 2 cut(s) 969, 2712
BsaXI ACNNNNNCTCC 8 cut(s) 1169, 1199, 1267, 1297, 2712, 2742, 3043, 3073
Bse118I RCCGGY 1 cut(s) 3197
Bse1I ACTGG 8 cut(s) 444, 724, 1032, 1759, 2221, 2344, 3208, 3280
Bse21I CCTNAGG 2 cut(s) 216, 843
Bse3DI GCAATG 5 cut(s) 904, 1489, 1965, 2331, 2857
BseBI CCWGG 5 cut(s) 663, 1739, 2379, 3093, 3156
BseDI CCNNGG 7 cut(s) 661, 831, 1557, 1676, 2377, 2619, 3154
BseMI GCAATG 5 cut(s) 904, 1489, 1965, 2331, 2857
BseNI ACTGG 8 cut(s) 444, 724, 1032, 1759, 2221, 2344, 3208, 3280
BseRI GAGGAG 5 cut(s) 233, 681, 1238, 1590, 2845
BseX3I CGGCCG 1 cut(s) 450
BseXI GCAGC 9 cut(s) 952, 1177, 1306, 1386, 1453, 1481, 2333, 2623, 2859
BseYI CCCAGC 1 cut(s) 3069
BsgI GTGCAG 2 cut(s) 632, 3159
Bsh1236I CGCG 1 cut(s) 2002
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 5 cut(s) 58, 443, 452, 1202, 2120
BsiEI CGRYCG 1 cut(s) 453
BsiHKAI GWGCWC 4 cut(s) 225, 262, 2529, 3383
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 4 cut(s) 970, 1252, 2713, 3198
BslFI GGGAC 7 cut(s) 197, 580, 601, 691, 1351, 1954, 2246
BsmAI GTCTC 5 cut(s) 660, 1551, 1722, 1853, 1904
BsmBI CGTCTC 1 cut(s) 1853
BsmFI GGGAC 7 cut(s) 197, 580, 601, 691, 1351, 1954, 2246
BsmI GAATGC 4 cut(s) 1623, 1921, 2302, 2323
BsnI GGCC 5 cut(s) 58, 443, 452, 1202, 2120
Bso31I GGTCTC 2 cut(s) 1551, 1904
BsoBI CYCGRG 1 cut(s) 643
Bsp1286I GDGCHC 5 cut(s) 225, 262, 1016, 2529, 3383
Bsp1407I TGTACA 1 cut(s) 741
Bsp143I GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
Bsp1720I GCTNAGC 2 cut(s) 1581, 2091
Bsp19I CCATGG 1 cut(s) 2619
BspANI GGCC 5 cut(s) 58, 443, 452, 1202, 2120
BspFNI CGCG 1 cut(s) 2002
BspHI TCATGA 3 cut(s) 751, 1990, 2953
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 2 cut(s) 1471, 2613
BspMI ACCTGC 2 cut(s) 1792, 2616
BspPI GGATC 2 cut(s) 578, 1305
BspQI GCTCTTC 1 cut(s) 924
BspTI CTTAAG 1 cut(s) 2660
BspTNI GGTCTC 2 cut(s) 1551, 1904
BsrDI GCAATG 5 cut(s) 904, 1489, 1965, 2331, 2857
BsrFI RCCGGY 1 cut(s) 3197
BsrGI TGTACA 1 cut(s) 741
BsrI ACTGG 8 cut(s) 444, 724, 1032, 1759, 2221, 2344, 3208, 3280
BssAI RCCGGY 1 cut(s) 3197
BssECI CCNNGG 7 cut(s) 661, 831, 1557, 1676, 2377, 2619, 3154
BssMI GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
BssT1I CCWWGG 2 cut(s) 1676, 2619
Bst2UI CCWGG 5 cut(s) 663, 1739, 2379, 3093, 3156
Bst4CI ACNGT 9 cut(s) 82, 150, 157, 694, 1442, 2126, 2946, 3138, 3363
Bst6I CTCTTC 3 cut(s) 924, 1128, 2656
BstAFI CTTAAG 1 cut(s) 2660
BstAPI GCANNNNNTGC 1 cut(s) 104
BstAUI TGTACA 1 cut(s) 741
BstBAI YACGTR 1 cut(s) 3384
BstC8I GCNNGC 9 cut(s) 284, 288, 570, 627, 1152, 1673, 2167, 2539, 2616
BstDSI CCRYGG 2 cut(s) 831, 2619
BstEII GGTNACC 2 cut(s) 972, 1457
BstENI CCTNNNNNAGG 2 cut(s) 1148, 3159
BstFNI CGCG 1 cut(s) 2002
BstHHI GCGC 1 cut(s) 2165
BstKTI GATC 9 cut(s) 78, 188, 586, 763, 786, 1300, 1631, 1824, 2922
BstMAI GTCTC 5 cut(s) 660, 1551, 1722, 1853, 1904
BstMBI GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 7 cut(s) 104, 440, 449, 635, 940, 1265, 1925
BstNI CCWGG 5 cut(s) 663, 1739, 2379, 3093, 3156
BstNSI RCATGY 8 cut(s) 286, 1003, 1246, 1344, 1839, 2169, 2680, 2790
BstPAI GACNNNNGTC 1 cut(s) 1355
BstPI GGTNACC 2 cut(s) 972, 1457
BstSCI CCNGG 6 cut(s) 661, 1251, 1737, 2377, 3091, 3154
BstSFI CTRYAG 5 cut(s) 1330, 1422, 1467, 2609, 2942
BstUI CGCG 1 cut(s) 2002
BstV1I GCAGC 9 cut(s) 952, 1177, 1306, 1386, 1453, 1481, 2333, 2623, 2859
BstX2I RGATCY 1 cut(s) 1297
BstXI CCANNNNNNTGG 1 cut(s) 3069
BstYI RGATCY 1 cut(s) 1297
BstZI CGGCCG 1 cut(s) 450
Bsu36I CCTNAGG 2 cut(s) 216, 843
BsuI GTATCC 2 cut(s) 2720, 2818
BsuRI GGCC 5 cut(s) 58, 443, 452, 1202, 2120
BtgI CCRYGG 2 cut(s) 831, 2619
BtsI GCAGTG 1 cut(s) 3171
BtsIMutI CAGTG 6 cut(s) 153, 437, 1025, 2109, 3171, 3215
BveI ACCTGC 2 cut(s) 1792, 2616
Cac8I GCNNGC 9 cut(s) 284, 288, 570, 627, 1152, 1673, 2167, 2539, 2616
CaiI CAGNNNCTG 4 cut(s) 125, 608, 715, 946
CciI TCATGA 3 cut(s) 751, 1990, 2953
CciNI GCGGCCGC 1 cut(s) 450
CfoI GCGC 1 cut(s) 2165
Cfr10I RCCGGY 1 cut(s) 3197
Cfr13I GGNCC 3 cut(s) 57, 1757, 3277
CseI GACGC 1 cut(s) 131
CsiI ACCWGGT 1 cut(s) 3091
Csp6I GTAC 5 cut(s) 360, 742, 1327, 1434, 2444
CviQI GTAC 5 cut(s) 360, 742, 1327, 1434, 2444
DpnI GATC 9 cut(s) 77, 187, 585, 762, 785, 1299, 1630, 1823, 2921
DpnII GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
DraI TTTAAA 1 cut(s) 135
EaeI YGGCCR 4 cut(s) 441, 450, 1200, 2118
EagI CGGCCG 1 cut(s) 450
Eam1104I CTCTTC 3 cut(s) 924, 1128, 2656
EarI CTCTTC 3 cut(s) 924, 1128, 2656
EclXI CGGCCG 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 1676, 2619
Eco24I GRGCYC 1 cut(s) 1016
Eco31I GGTCTC 2 cut(s) 1551, 1904
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 2 cut(s) 1757, 3277
Eco52I CGGCCG 1 cut(s) 450
Eco57I CTGAAG 6 cut(s) 188, 635, 1166, 1424, 1470, 2993
Eco81I CCTNAGG 2 cut(s) 216, 843
Eco88I CYCGRG 1 cut(s) 643
Eco91I GGTNACC 2 cut(s) 972, 1457
EcoNI CCTNNNNNAGG 2 cut(s) 1148, 3159
EcoO65I GGTNACC 2 cut(s) 972, 1457
EcoRI GAATTC 3 cut(s) 958, 1139, 3165
EcoRII CCWGG 5 cut(s) 661, 1737, 2377, 3091, 3154
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 1676, 2619
EcoT22I ATGCAT 1 cut(s) 2788
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 2 cut(s) 1676, 2619
Esp3I CGTCTC 1 cut(s) 1853
FalI AAGNNNNNCTT 6 cut(s) 1660, 1692, 3213, 3245, 3293, 3325
FaqI GGGAC 7 cut(s) 197, 580, 601, 691, 1351, 1954, 2246
FauI CCCGC 1 cut(s) 1112
FbaI TGATCA 2 cut(s) 75, 760
FriOI GRGCYC 1 cut(s) 1016
FspBI CTAG 6 cut(s) 498, 1287, 1478, 1665, 1785, 2201
GlaI GCGC 1 cut(s) 2164
GsaI CCCAGC 1 cut(s) 3073
GsuI CTGGAG 4 cut(s) 684, 956, 2400, 3045
HaeIII GGCC 5 cut(s) 58, 443, 452, 1202, 2120
HapII CCGG 4 cut(s) 970, 1252, 2713, 3198
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 2165
Hin6I GCGC 1 cut(s) 2163
HinP1I GCGC 1 cut(s) 2163
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HindIII AAGCTT 3 cut(s) 623, 1067, 1669
HpaII CCGG 4 cut(s) 970, 1252, 2713, 3198
Hpy166II GTNNAC 6 cut(s) 153, 417, 742, 1350, 2718, 3134
Hpy8I GTNNAC 6 cut(s) 153, 417, 742, 1350, 2718, 3134
HpyAV CCTTC 6 cut(s) 371, 2501, 2618, 2837, 3066, 3218
HpyCH4III ACNGT 9 cut(s) 82, 150, 157, 694, 1442, 2126, 2946, 3138, 3363
HpyCH4IV ACGT 3 cut(s) 51, 305, 3383
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 440, 449, 635, 940, 1265, 1925
HpySE526I ACGT 3 cut(s) 51, 305, 3383
HspAI GCGC 1 cut(s) 2163
Ksp22I TGATCA 2 cut(s) 75, 760
Kzo9I GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
LguI GCTCTTC 1 cut(s) 924
Lsp1109I GCAGC 9 cut(s) 952, 1177, 1306, 1386, 1453, 1481, 2333, 2623, 2859
MabI ACCWGGT 1 cut(s) 3091
MaeI CTAG 6 cut(s) 498, 1287, 1478, 1665, 1785, 2201
MaeII ACGT 3 cut(s) 51, 305, 3383
MalI GATC 9 cut(s) 77, 187, 585, 762, 785, 1299, 1630, 1823, 2921
MboI GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
MboII GAAGA 9 cut(s) 767, 941, 1115, 1222, 1417, 1626, 2643, 2882, 2978
MfeI CAATTG 3 cut(s) 291, 1196, 2405
MflI RGATCY 1 cut(s) 1297
MhlI GDGCHC 5 cut(s) 225, 262, 1016, 2529, 3383
MlsI TGGCCA 2 cut(s) 443, 2120
MluNI TGGCCA 2 cut(s) 443, 2120
MmeI TCCRAC 2 cut(s) 774, 1029
Mox20I TGGCCA 2 cut(s) 443, 2120
Mph1103I ATGCAT 1 cut(s) 2788
MroXI GAANNNNTTC 3 cut(s) 1516, 2481, 2874
MscI TGGCCA 2 cut(s) 443, 2120
MslI CAYNNNNRTG 2 cut(s) 114, 756
Msp20I TGGCCA 2 cut(s) 443, 2120
MspCI CTTAAG 1 cut(s) 2660
MspI CCGG 4 cut(s) 970, 1252, 2713, 3198
MspR9I CCNGG 6 cut(s) 663, 1253, 1739, 2379, 3093, 3156
MunI CAATTG 3 cut(s) 291, 1196, 2405
Mva1269I GAATGC 4 cut(s) 1623, 1921, 2302, 2323
MvaI CCWGG 5 cut(s) 663, 1739, 2379, 3093, 3156
MvnI CGCG 1 cut(s) 2002
MwoI GCNNNNNNNGC 7 cut(s) 104, 440, 449, 635, 940, 1265, 1925
NciI CCSGG 1 cut(s) 1253
NcoI CCATGG 1 cut(s) 2619
NdeII GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 1417, 1582
NmuCI GTSAC 5 cut(s) 972, 1238, 1357, 1371, 3363
NotI GCGGCCGC 1 cut(s) 450
NsiI ATGCAT 1 cut(s) 2788
NspI RCATGY 8 cut(s) 286, 1003, 1246, 1344, 1839, 2169, 2680, 2790
PaeI GCATGC 2 cut(s) 286, 2169
PaeR7I CTCGAG 1 cut(s) 643
PagI TCATGA 3 cut(s) 751, 1990, 2953
PaqCI CACCTGC 1 cut(s) 1792
PciI ACATGT 2 cut(s) 999, 1340
PciSI GCTCTTC 1 cut(s) 924
PctI GAATGC 4 cut(s) 1623, 1921, 2302, 2323
PdmI GAANNNNTTC 3 cut(s) 1516, 2481, 2874
PflFI GACNNNGTC 1 cut(s) 731
PflMI CCANNNNNTGG 2 cut(s) 1071, 1744
PfoI TCCNGGA 2 cut(s) 1251, 1737
Ppu21I YACGTR 1 cut(s) 3384
PscI ACATGT 2 cut(s) 999, 1340
PshAI GACNNNNGTC 1 cut(s) 1355
PshBI ATTAAT 1 cut(s) 1695
Psp6I CCWGG 5 cut(s) 661, 1737, 2377, 3091, 3154
PspEI GGTNACC 2 cut(s) 972, 1457
PspFI CCCAGC 1 cut(s) 3069
PspGI CCWGG 5 cut(s) 661, 1737, 2377, 3091, 3154
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 57, 1757, 3277
PsrI GAACNNNNNNTAC 2 cut(s) 7, 39
PstI CTGCAG 2 cut(s) 1471, 2613
PstNI CAGNNNCTG 4 cut(s) 125, 608, 715, 946
PsuI RGATCY 1 cut(s) 1297
PsyI GACNNNGTC 1 cut(s) 731
RsaI GTAC 5 cut(s) 361, 743, 1328, 1435, 2445
RsaNI GTAC 5 cut(s) 360, 742, 1327, 1434, 2444
RseI CAYNNNNRTG 2 cut(s) 114, 756
SapI GCTCTTC 1 cut(s) 924
Sau3AI GATC 9 cut(s) 75, 185, 583, 760, 783, 1297, 1628, 1821, 2919
Sau96I GGNCC 3 cut(s) 57, 1757, 3277
ScaI AGTACT 1 cut(s) 1435
ScrFI CCNGG 6 cut(s) 663, 1253, 1739, 2379, 3093, 3156
SduI GDGCHC 5 cut(s) 225, 262, 1016, 2529, 3383
SexAI ACCWGGT 1 cut(s) 3091
SfcI CTRYAG 5 cut(s) 1330, 1422, 1467, 2609, 2942
SfiI GGCCNNNNNGGCC 1 cut(s) 449
Sfr274I CTCGAG 1 cut(s) 643
SgrAI CRCCGGYG 1 cut(s) 3197
SinI GGWCC 2 cut(s) 1757, 3277
SlaI CTCGAG 1 cut(s) 643
SmiMI CAYNNNNRTG 2 cut(s) 114, 756
SmlI CTYRAG 5 cut(s) 643, 1866, 2660, 2813, 2881
SmoI CTYRAG 5 cut(s) 643, 1866, 2660, 2813, 2881
SpeI ACTAGT 1 cut(s) 2200
SphI GCATGC 2 cut(s) 286, 2169
SspI AATATT 2 cut(s) 2059, 2280
SspMI CTAG 6 cut(s) 498, 1287, 1478, 1665, 1785, 2201
StyD4I CCNGG 6 cut(s) 661, 1251, 1737, 2377, 3091, 3154
StyI CCWWGG 2 cut(s) 1676, 2619
TaaI ACNGT 9 cut(s) 82, 150, 157, 694, 1442, 2126, 2946, 3138, 3363
TaiI ACGT 3 cut(s) 54, 308, 3386
TaqI TCGA 2 cut(s) 458, 644
TatI WGTACW 4 cut(s) 741, 1326, 1433, 2443
TauI GCSGC 3 cut(s) 452, 455, 851
TscAI CASTG 6 cut(s) 160, 444, 1032, 2109, 3178, 3215
TseFI GTSAC 5 cut(s) 972, 1238, 1357, 1371, 3363
TseI GCWGC 9 cut(s) 940, 1165, 1319, 1399, 1466, 1469, 2321, 2611, 2847
Tsp45I GTSAC 5 cut(s) 972, 1238, 1357, 1371, 3363
TspDTI ATGAA 8 cut(s) 185, 312, 740, 768, 1979, 2705, 2942, 3281
TspGWI ACGGA 4 cut(s) 848, 2222, 3119, 3352
TspRI CASTG 6 cut(s) 160, 444, 1032, 2109, 3178, 3215
Tth111I GACNNNGTC 1 cut(s) 731
Van91I CCANNNNNTGG 2 cut(s) 1071, 1744
Vha464I CTTAAG 1 cut(s) 2660
VpaK11BI GGWCC 2 cut(s) 1757, 3277
VspI ATTAAT 1 cut(s) 1695
XagI CCTNNNNNAGG 2 cut(s) 1148, 3159
XapI RAATTY 9 cut(s) 958, 1139, 2077, 2213, 2432, 2645, 2873, 3084, 3165
XceI RCATGY 8 cut(s) 286, 1003, 1246, 1344, 1839, 2169, 2680, 2790
XhoI CTCGAG 1 cut(s) 643
XmnI GAANNNNTTC 3 cut(s) 1516, 2481, 2874
XspI CTAG 6 cut(s) 498, 1287, 1478, 1665, 1785, 2201
ZrmI AGTACT 1 cut(s) 1435
Zsp2I ATGCAT 1 cut(s) 2788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.