Rh5CG108400

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007664
Physical Location & Seq
Reverse (-)
8942338 .. 8947140
4803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG108400.1

Sequence Viewer

Length: 2892 bp
ATGTCCAAATTACTTGGGAAGCGTTTCAACAATAAAAACATGGAGCCTGAGAGAACCACCGGTACATTCTCATCCTCAGCTACAAGCAACGTGAGGCCCAACAACAACACCATGATCACCGTCAAAAGAGATAAAGCAATTATTGCTCACTACAATGCAGACGCTGGACTTTTAAATGAGTTTGAACAGTCCACTGTGTCTGGTAAGTCCTTTAACTATACAAGATCAATGCTTCATCCACTTCAGTCTGTCCCTGAGGAGCACATAACTGCTTATTTCTCAAAAATCCAAAGAGGCGCTCTTGTTCAGTCTTTTGGGTGCATGCTTGCAATTGATGAACCTACGTTTAGGATTATCTGTTATAGTGAGAACTGCTCTGAGTTTTTGGGTTTGGATGGTATTTTTGAGTCAAAAGAAGAAAAGGGTCTGATTGGAATTGATGCTAAAACTCTGTTTTCACCCTCATCTGGGGCTTCACTGGCCAAAGCGGCCGCTTCGACGGAGCTATCATTGCTGAACCCAATTTGGGTGTATTCTAGGACCACCCAAAAGCCATTTTATGCTATATTGCATAGGATTGATGTTGGGATTGTGATTGATTTAGAGCCTGCCAAGTGTGGTGATCCTGCATTGTCCCTTGCAGGGGCTGTGCAGTCCCAGAAGCTTGCGGTTCGGGCAATCTCGAGGCTTCAGTCTCTCCCTGGAGGAGATATTGGGACTCTGTGTGACACTGTTGCGGAGGATGTTCAGAAGCTGACCGGATATGACAGAGTTATGGTGTACAAGTTTCATGAAGATGATCATGGGGAAGTTGTGTCTGAGATCAGAAGGTCGGATTTGGAGTCCTATTTGGGGTTGCATTATCCTGCCACGGATATCCCTCAGGCGGCTCGGTTCTTGTTCAAGCAGAATCGGGTGCGCATGATTTGTGATTGCAATGCAAATCCTGTAAAACTCATTCAAAGTGAAGAGCTGGAGCAGCCTCTGTGCTTGGTGAATTCAACTCTCCGGTCACCACACGATTGCCACAGAACATACATGTTAAACATGGGCTCAGTTGCATCACTGGTCATGGCGGTTACTATCAATAGTCCTGATTCCACCAAGCTTTGGGGGTTGGTGGTGTGCCATCACACTTCACCCCGCTATGTTCCTTTCCCTCTTCGCTATGCTTGTGAATTTCTTATGCAGGCATTTGTGTTGCAGCTTAACATGGAGCTTCAGTTGGCATTACAATTGGCCGAAAAGAAGATTCTCAAAACACAGACTTTACTTTGTGACATGCTCCTCCGGGATGCCCCGTCTGGTATTGTAACCCAATCTCCTAGTATAATGGATCTTGTCAAGTCTGATGGGGCTGCATTGTACTATAGTGGGACGTGTTGGTTGACGGGTGTCACACCAACTGAGTCACAGGTTAAAGATATAGCCGAGTGGCTGCTGAAGAATCATGGGGATTCTACAGGACTGAGTACTGACAGTTTGGCTGAAGCTGGTTACCCTGCTGCAGCTCTACTAGGCAATGCGGTTTGTGGTATGGCTACTGCAAGAATCAGTTCAAACGATTTTCTGTTTTGGTTCAGGTCTCACACCGCCGAGGAAGTCAAGTGGGGAGGAGCTAAGCATCATCCACAGGATAAGGATGATGGTGGAAGAATGCACCCAAGATCATCATTTAAGGCTTTTCTTGAAGTAGCGAAAAGTAGAAGCTTGCCTTGGGAGGACTCCGAGATTAATGCCATCCACTCTTTACAGCTCATAATGAGAGACTCGTTCCAGGATATGGAGGAAACTGGTCCTAAGCCAATAAATAATAATGCTCGTCCTGGTGATGCTGAGATGCAGGTGGAGGGGATGGATGAGATCAGTTCTGTGGCATGTGAAATGGTTAAATTGATTGAGACGGCTCAAGTTCCGATTTTCGGGGTTGATTCAGAGGGTCTCATCAATGGATGGAATGCAAAGATGGCTGAATTGACAGGATTACAAGATGGTGAAGCAATGGGGAAGTCCCTTGTTAATGATATTGTTCATGAGGACTCGCGTGGAGCTGTTGAAAGTATCTTATCCAGAGCGTTACAAGGTGATGAGGACAAGAATATTGAGTTGAAACTGAGAAATTTTGGACTTGCTCAGCAAAACTCAGTGGTATATATCGTGGCCAACAGTTGCACAAGTAGGAATCATGCAAAGAATGTTGTTGGCGCATGCTTCGTGGGTCAAGACATTACTTGTGAAAAACTTGTTACGGATAAATTTATCCAGTTGCAAGGGGACTATAGGGCTATCATACAGAGTCCTAGCCCATTGATTCCACCAATATTTGCATCTGATGAGAATGCCTACTGCTCTGAATGGAATGCAGCAATGGAAAAGCTGACTGGTTTGACAAGAGATGATGTTATTGGTAAAATGCTCCCTGGAGAAATCTTTGGAGAGTTCTGTCAATTGAAAGGTCAAGATTCGCTAACCAAATTTAGGATTGTACTATATCAAGGAATCATTGGGCAAGACATTGAAAAGTTTCCATTTGGGTTTTTTGATAGAGAAGGGAAGTATGTTGAGGTGCTCTTAACAGCAAGCAAGAGGACTGATGCCAATGGGAATATAGTTGGTTGCTTCTGCTTCTTGCAAACATTTCTACCAGACCTGCAGCCAGCCATGGATGGACTCGGACAAGAGGATAAATTCTCTTCAAAACTTAAGGAATTAACTTACATGCGACAAGAGATGAAAAATCCTTTAAGTGGTATCCGGTTTACACACAGACTCCTTGAAAATACAAATGTTTCAGAATATCAGAAGCAATTTCTTGACACTAGTGATGCATGTGAGAGACAAATCATGGCAATCCTTGAGAATCTGGATACGAGGAGCATAGAGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

963

Amino Acids

106.7

Weight (kDa)

5.29

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 92 - 204 1e-33 PAS fold
GAF PF01590 237 - 407 3.6e-17 GAF domain
PHY PF00360 420 - 595 9.8e-57 Phytochrome region
PAS PF00989 628 - 743 1.7e-24 PAS fold
PAS_4 PF08448 635 - 744 7.7e-07 PAS fold
PAS PF00989 759 - 878 3.3e-18 PAS fold
HisKA PF00512 900 - 962 1.1e-09 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1834
Acc16I TGCGCA 1 cut(s) 920
Acc36I ACCTGC 2 cut(s) 1834, 2658
AccB7I CCANNNNNTGG 2 cut(s) 1110, 1783
AccII CGCG 1 cut(s) 2044
AciI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
AclWI GGATC 2 cut(s) 617, 1344
AcoI YGGCCR 4 cut(s) 480, 489, 1239, 2160
AcsI RAATTY 6 cut(s) 997, 1178, 2119, 2255, 2474, 2687
AcuI CTGAAG 5 cut(s) 227, 674, 1205, 1463, 1509
AfaI GTAC 5 cut(s) 64, 782, 1367, 1474, 2487
AflII CTTAAG 1 cut(s) 2702
AflIII ACRYGT 2 cut(s) 1038, 1379
AgeI ACCGGT 1 cut(s) 59
AhlI ACTAGT 1 cut(s) 2819
AjiI CACGTC 1 cut(s) 1380
AjnI CCWGG 4 cut(s) 700, 1776, 1825, 2419
AjuI GAANNNNNNNTTGG 2 cut(s) 1699, 1731
Alw21I GWGCWC 2 cut(s) 264, 2571
Alw26I GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
AlwI GGATC 2 cut(s) 617, 1344
AlwNI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
Ama87I CYCGRG 1 cut(s) 682
AoxI GGCC 5 cut(s) 95, 480, 489, 1239, 2160
ApeKI GCWGC 8 cut(s) 979, 1204, 1358, 1438, 1505, 1508, 2363, 2653
ApoI RAATTY 6 cut(s) 997, 1178, 2119, 2255, 2474, 2687
AseI ATTAAT 1 cut(s) 1734
AsiGI ACCGGT 1 cut(s) 59
Asp700I GAANNNNTTC 3 cut(s) 23, 1555, 2523
AspLEI GCGC 3 cut(s) 299, 921, 2207
AspS9I GGNCC 3 cut(s) 96, 540, 1796
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 9 cut(s) 109, 450, 632, 1005, 1006, 1131, 1841, 2006, 2096
AvaI CYCGRG 1 cut(s) 682
AvaII GGWCC 2 cut(s) 540, 1796
AxyI CCTNAGG 2 cut(s) 255, 882
BalI TGGCCA 2 cut(s) 482, 2162
BanII GRGCYC 1 cut(s) 1055
Bbv12I GWGCWC 2 cut(s) 264, 2571
BbvCI CCTCAGC 1 cut(s) 76
BbvI GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BceAI ACGGC 1 cut(s) 1920
BcgI CGANNNNNNTGC 2 cut(s) 1718, 1752
BciT130I CCWGG 4 cut(s) 702, 1778, 1827, 2421
BciVI GTATCC 2 cut(s) 2762, 2860
BclI TGATCA 2 cut(s) 114, 799
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BcuI ACTAGT 1 cut(s) 2819
BfaI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
BfmI CTRYAG 5 cut(s) 1369, 1461, 1506, 2278, 2651
BfoI RGCGCY 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 2702
BfuAI ACCTGC 2 cut(s) 1834, 2658
BfuI GTATCC 2 cut(s) 2762, 2860
BglI GCCNNNNNGGC 1 cut(s) 488
BlpI GCTNAGC 2 cut(s) 1620, 2133
BmcAI AGTACT 1 cut(s) 1474
Bme1390I CCNGG 5 cut(s) 702, 1292, 1778, 1827, 2421
Bme18I GGWCC 2 cut(s) 540, 1796
BmeT110I CYCGRG 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 1380
BmgT120I GGNCC 3 cut(s) 96, 540, 1796
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 5 cut(s) 702, 1292, 1778, 1827, 2421
BmsI GCATC 9 cut(s) 430, 1070, 1285, 1633, 1822, 1830, 2336, 2584, 2815
BoxI GACNNNNGTC 1 cut(s) 1394
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 3 cut(s) 723, 995, 2442
Bpu10I CCTNAGC 2 cut(s) 76, 1800
Bpu1102I GCTNAGC 2 cut(s) 1620, 2133
BpuEI CTTGAG 2 cut(s) 1893, 2876
BpuMI CCSGG 1 cut(s) 1292
BsaI GGTCTC 2 cut(s) 1590, 1946
BsaJI CCNNGG 6 cut(s) 700, 870, 1596, 1715, 2419, 2661
BsaWI WCCGGW 4 cut(s) 59, 758, 1008, 2754
BsaXI ACNNNNNCTCC 6 cut(s) 1208, 1238, 1306, 1336, 2754, 2784
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 5 cut(s) 483, 1071, 1798, 2263, 2386
Bse21I CCTNAGG 2 cut(s) 255, 882
Bse3DI GCAATG 5 cut(s) 509, 943, 1528, 2007, 2373
BseBI CCWGG 4 cut(s) 702, 1778, 1827, 2421
BseDI CCNNGG 6 cut(s) 700, 870, 1596, 1715, 2419, 2661
BseMI GCAATG 5 cut(s) 509, 943, 1528, 2007, 2373
BseNI ACTGG 5 cut(s) 483, 1071, 1798, 2263, 2386
BseRI GAGGAG 5 cut(s) 272, 720, 1277, 1629, 2887
BseX3I CGGCCG 1 cut(s) 489
BseXI GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BsgI GTGCAG 1 cut(s) 671
Bsh1236I CGCG 1 cut(s) 2044
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BshTI ACCGGT 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 264, 2571
BsiHKCI CYCGRG 1 cut(s) 682
BsiSI CCGG 5 cut(s) 60, 759, 1009, 1291, 2755
BslFI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
BsmAI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
BsmI GAATGC 4 cut(s) 1662, 1963, 2344, 2365
BsnI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
Bso31I GGTCTC 2 cut(s) 1590, 1946
BsoBI CYCGRG 1 cut(s) 682
Bsp1286I GDGCHC 3 cut(s) 264, 1055, 2571
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
Bsp1720I GCTNAGC 2 cut(s) 1620, 2133
Bsp19I CCATGG 1 cut(s) 2661
BspACI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
BspANI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BspFNI CGCG 1 cut(s) 2044
BspHI TCATGA 2 cut(s) 790, 2032
BspLI GGNNCC 1 cut(s) 45
BspMAI CTGCAG 2 cut(s) 1510, 2655
BspMI ACCTGC 2 cut(s) 1834, 2658
BspPI GGATC 2 cut(s) 617, 1344
BspQI GCTCTTC 1 cut(s) 963
BspTI CTTAAG 1 cut(s) 2702
BspTNI GGTCTC 2 cut(s) 1590, 1946
BsrDI GCAATG 5 cut(s) 509, 943, 1528, 2007, 2373
BsrFI RCCGGY 1 cut(s) 59
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 5 cut(s) 483, 1071, 1798, 2263, 2386
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 6 cut(s) 700, 870, 1596, 1715, 2419, 2661
BssMI GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
BssT1I CCWWGG 2 cut(s) 1715, 2661
Bst2UI CCWGG 4 cut(s) 702, 1778, 1827, 2421
Bst4CI ACNGT 6 cut(s) 121, 189, 196, 733, 1481, 2168
Bst6I CTCTTC 3 cut(s) 963, 1167, 2698
BstAFI CTTAAG 1 cut(s) 2702
BstAPI GCANNNNNTGC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 780
BstC8I GCNNGC 9 cut(s) 323, 327, 609, 666, 1191, 1712, 2209, 2581, 2658
BstDSI CCRYGG 2 cut(s) 870, 2661
BstEII GGTNACC 2 cut(s) 1011, 1496
BstFNI CGCG 1 cut(s) 2044
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 3 cut(s) 299, 921, 2207
BstKTI GATC 8 cut(s) 117, 227, 625, 802, 825, 1339, 1670, 1866
BstMAI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BstMBI GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
BstNI CCWGG 4 cut(s) 702, 1778, 1827, 2421
BstNSI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
BstPAI GACNNNNGTC 1 cut(s) 1394
BstPI GGTNACC 2 cut(s) 1011, 1496
BstSCI CCNGG 5 cut(s) 700, 1290, 1776, 1825, 2419
BstSFI CTRYAG 5 cut(s) 1369, 1461, 1506, 2278, 2651
BstUI CGCG 1 cut(s) 2044
BstV1I GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BstX2I RGATCY 1 cut(s) 1336
BstYI RGATCY 1 cut(s) 1336
BstZI CGGCCG 1 cut(s) 489
Bsu36I CCTNAGG 2 cut(s) 255, 882
BsuI GTATCC 2 cut(s) 2762, 2860
BsuRI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BtgI CCRYGG 2 cut(s) 870, 2661
BtrI CACGTC 1 cut(s) 1380
BtsIMutI CAGTG 5 cut(s) 192, 476, 729, 1064, 2151
BveI ACCTGC 2 cut(s) 1834, 2658
Cac8I GCNNGC 9 cut(s) 323, 327, 609, 666, 1191, 1712, 2209, 2581, 2658
CaiI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
CciI TCATGA 2 cut(s) 790, 2032
CciNI GCGGCCGC 1 cut(s) 489
CfoI GCGC 3 cut(s) 299, 921, 2207
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 96, 540, 1796
CseI GACGC 1 cut(s) 170
Csp6I GTAC 5 cut(s) 63, 781, 1366, 1473, 2486
CspAI ACCGGT 1 cut(s) 59
CviQI GTAC 5 cut(s) 63, 781, 1366, 1473, 2486
DpnI GATC 8 cut(s) 116, 226, 624, 801, 824, 1338, 1669, 1865
DpnII GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 4 cut(s) 480, 489, 1239, 2160
EagI CGGCCG 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 963, 1167, 2698
EarI CTCTTC 3 cut(s) 963, 1167, 2698
EclXI CGGCCG 1 cut(s) 489
Eco130I CCWWGG 2 cut(s) 1715, 2661
Eco24I GRGCYC 1 cut(s) 1055
Eco31I GGTCTC 2 cut(s) 1590, 1946
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 2 cut(s) 540, 1796
Eco52I CGGCCG 1 cut(s) 489
Eco57I CTGAAG 5 cut(s) 227, 674, 1205, 1463, 1509
Eco81I CCTNAGG 2 cut(s) 255, 882
Eco88I CYCGRG 1 cut(s) 682
Eco91I GGTNACC 2 cut(s) 1011, 1496
EcoO65I GGTNACC 2 cut(s) 1011, 1496
EcoRI GAATTC 1 cut(s) 997
EcoRII CCWGG 4 cut(s) 700, 1776, 1825, 2419
EcoRV GATATC 1 cut(s) 877
EcoT14I CCWWGG 2 cut(s) 1715, 2661
EcoT22I ATGCAT 1 cut(s) 2830
EcoT38I GRGCYC 1 cut(s) 1055
ErhI CCWWGG 2 cut(s) 1715, 2661
Esp3I CGTCTC 1 cut(s) 1895
FalI AAGNNNNNCTT 2 cut(s) 1699, 1731
FaqI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
FauI CCCGC 1 cut(s) 1151
FbaI TGATCA 2 cut(s) 114, 799
FriOI GRGCYC 1 cut(s) 1055
FspAI RTGCGCAY 1 cut(s) 920
FspBI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
FspI TGCGCA 1 cut(s) 920
GlaI GCGC 3 cut(s) 298, 920, 2206
GsuI CTGGAG 3 cut(s) 723, 995, 2442
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 5 cut(s) 97, 482, 491, 1241, 2162
HapII CCGG 5 cut(s) 60, 759, 1009, 1291, 2755
HgaI GACGC 1 cut(s) 170
HhaI GCGC 3 cut(s) 299, 921, 2207
Hin6I GCGC 3 cut(s) 297, 919, 2205
HinP1I GCGC 3 cut(s) 297, 919, 2205
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HindIII AAGCTT 3 cut(s) 662, 1106, 1708
HpaII CCGG 5 cut(s) 60, 759, 1009, 1291, 2755
HphI GGTGA 9 cut(s) 109, 450, 632, 1005, 1006, 1131, 1841, 2006, 2096
Hpy166II GTNNAC 4 cut(s) 192, 781, 1389, 2760
Hpy8I GTNNAC 4 cut(s) 192, 781, 1389, 2760
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 3 cut(s) 822, 2543, 2879
HpyCH4III ACNGT 6 cut(s) 121, 189, 196, 733, 1481, 2168
HpyCH4IV ACGT 3 cut(s) 90, 344, 1379
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
HpySE526I ACGT 3 cut(s) 90, 344, 1379
HspAI GCGC 3 cut(s) 297, 919, 2205
Ksp22I TGATCA 2 cut(s) 114, 799
Kzo9I GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
LguI GCTCTTC 1 cut(s) 963
Lsp1109I GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
LweI GCATC 9 cut(s) 430, 1070, 1285, 1633, 1822, 1830, 2336, 2584, 2815
MaeI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
MaeII ACGT 3 cut(s) 90, 344, 1379
MalI GATC 8 cut(s) 116, 226, 624, 801, 824, 1338, 1669, 1865
MboI GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
MboII GAAGA 8 cut(s) 428, 806, 980, 1154, 1261, 1456, 1665, 2685
MfeI CAATTG 3 cut(s) 330, 1235, 2447
MflI RGATCY 1 cut(s) 1336
MhlI GDGCHC 3 cut(s) 264, 1055, 2571
MlsI TGGCCA 2 cut(s) 482, 2162
MluNI TGGCCA 2 cut(s) 482, 2162
MmeI TCCRAC 1 cut(s) 813
Mox20I TGGCCA 2 cut(s) 482, 2162
Mph1103I ATGCAT 1 cut(s) 2830
MroXI GAANNNNTTC 3 cut(s) 23, 1555, 2523
MscI TGGCCA 2 cut(s) 482, 2162
MslI CAYNNNNRTG 2 cut(s) 153, 795
Msp20I TGGCCA 2 cut(s) 482, 2162
MspCI CTTAAG 1 cut(s) 2702
MspI CCGG 5 cut(s) 60, 759, 1009, 1291, 2755
MspR9I CCNGG 5 cut(s) 702, 1292, 1778, 1827, 2421
MunI CAATTG 3 cut(s) 330, 1235, 2447
Mva1269I GAATGC 4 cut(s) 1662, 1963, 2344, 2365
MvaI CCWGG 4 cut(s) 702, 1778, 1827, 2421
MvnI CGCG 1 cut(s) 2044
MwoI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 2661
NdeII GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
NlaIV GGNNCC 1 cut(s) 45
NmeAIII GCCGAG 2 cut(s) 1456, 1621
NmuCI GTSAC 5 cut(s) 725, 1011, 1277, 1396, 1410
NotI GCGGCCGC 1 cut(s) 489
NsbI TGCGCA 1 cut(s) 920
NsiI ATGCAT 1 cut(s) 2830
NspI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
PaeI GCATGC 2 cut(s) 325, 2211
PaeR7I CTCGAG 1 cut(s) 682
PagI TCATGA 2 cut(s) 790, 2032
PaqCI CACCTGC 1 cut(s) 1834
PciI ACATGT 1 cut(s) 1038
PciSI GCTCTTC 1 cut(s) 963
PctI GAATGC 4 cut(s) 1662, 1963, 2344, 2365
PdmI GAANNNNTTC 3 cut(s) 23, 1555, 2523
PflMI CCANNNNNTGG 2 cut(s) 1110, 1783
PfoI TCCNGGA 2 cut(s) 1290, 1776
PinAI ACCGGT 1 cut(s) 59
PscI ACATGT 1 cut(s) 1038
PshAI GACNNNNGTC 1 cut(s) 1394
PshBI ATTAAT 1 cut(s) 1734
Psp6I CCWGG 4 cut(s) 700, 1776, 1825, 2419
PspEI GGTNACC 2 cut(s) 1011, 1496
PspGI CCWGG 4 cut(s) 700, 1776, 1825, 2419
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 3 cut(s) 96, 540, 1796
PsrI GAACNNNNNNTAC 2 cut(s) 46, 78
PstI CTGCAG 2 cut(s) 1510, 2655
PstNI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
PsuI RGATCY 1 cut(s) 1336
RsaI GTAC 5 cut(s) 64, 782, 1367, 1474, 2487
RsaNI GTAC 5 cut(s) 63, 781, 1366, 1473, 2486
RseI CAYNNNNRTG 2 cut(s) 153, 795
SapI GCTCTTC 1 cut(s) 963
Sau3AI GATC 8 cut(s) 114, 224, 622, 799, 822, 1336, 1667, 1863
Sau96I GGNCC 3 cut(s) 96, 540, 1796
ScaI AGTACT 1 cut(s) 1474
ScrFI CCNGG 5 cut(s) 702, 1292, 1778, 1827, 2421
SduI GDGCHC 3 cut(s) 264, 1055, 2571
SfaNI GCATC 9 cut(s) 430, 1070, 1285, 1633, 1822, 1830, 2336, 2584, 2815
SfcI CTRYAG 5 cut(s) 1369, 1461, 1506, 2278, 2651
SfiI GGCCNNNNNGGCC 1 cut(s) 488
Sfr274I CTCGAG 1 cut(s) 682
SinI GGWCC 2 cut(s) 540, 1796
SlaI CTCGAG 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 153, 795
SmlI CTYRAG 4 cut(s) 682, 1908, 2702, 2855
SmoI CTYRAG 4 cut(s) 682, 1908, 2702, 2855
SpeI ACTAGT 1 cut(s) 2819
SphI GCATGC 2 cut(s) 325, 2211
SsiI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
SspI AATATT 2 cut(s) 2101, 2322
SspMI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
StyD4I CCNGG 5 cut(s) 700, 1290, 1776, 1825, 2419
StyI CCWWGG 2 cut(s) 1715, 2661
TaaI ACNGT 6 cut(s) 121, 189, 196, 733, 1481, 2168
TaiI ACGT 3 cut(s) 93, 347, 1382
TaqI TCGA 2 cut(s) 497, 683
TatI WGTACW 4 cut(s) 780, 1365, 1472, 2485
TauI GCSGC 3 cut(s) 491, 494, 890
TscAI CASTG 5 cut(s) 199, 483, 736, 1071, 2151
TseFI GTSAC 5 cut(s) 725, 1011, 1277, 1396, 1410
TseI GCWGC 8 cut(s) 979, 1204, 1358, 1438, 1505, 1508, 2363, 2653
Tsp45I GTSAC 5 cut(s) 725, 1011, 1277, 1396, 1410
TspDTI ATGAA 6 cut(s) 224, 351, 779, 807, 2021, 2747
TspGWI ACGGA 3 cut(s) 515, 887, 2264
TspRI CASTG 5 cut(s) 199, 483, 736, 1071, 2151
Van91I CCANNNNNTGG 2 cut(s) 1110, 1783
Vha464I CTTAAG 1 cut(s) 2702
VpaK11BI GGWCC 2 cut(s) 540, 1796
VspI ATTAAT 1 cut(s) 1734
XapI RAATTY 6 cut(s) 997, 1178, 2119, 2255, 2474, 2687
XceI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
XhoI CTCGAG 1 cut(s) 682
XmnI GAANNNNTTC 3 cut(s) 23, 1555, 2523
XspI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
ZrmI AGTACT 1 cut(s) 1474
Zsp2I ATGCAT 1 cut(s) 2830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.