MD10G1267600.v1.1

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007664
Physical Location & Seq
Forward (+)
36059450 .. 36063715
4266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1267600.v1.1.491

Sequence Viewer

Length: 3399 bp
ATGGAGCTTGGCAAAAGCAGTGACAGAACACGCGCCGCCGCATTCTCCTCCTCCTCCGCCACAAGCAACATGAAACCCACCCCCGCGGCCGCCAAACCCGATAGGTCAATCGCTCACTACAGAGAGGACGCCAACCTCTTGGCCGAGTACGAGCAGTCTGCCGTCTCCGGTACGCCCTTCCACTACACAAGGTCAGTGCTTTTTCCTCCGGATTCTGTTCCCGAGGAGCAAATCGTTGCCTACCTTTCGAGAATCCAAAGGGGTTCTCTCGTCCAATCTTTTGGCTGTATGCTTGCAATTAAAGAACCCACTTTTCGAATTATCGGTTTTAGTGAGAATTGCTTTGAATTGCTGGGTCTGGATAAATCGGGTAGTGTTTTTGGGTCAAATGAGTTAAAGGGTTTGATTGGAATTGACTGTAGAACTCTGTTTACTCCTTCTTCCGCGGCTTCTTTGGCGAAAGCTGCTGCTTCGAGGGAGATTTCGCTGTTAAACCCGGTTTGGGTGTATTCTAGGAGCACCCAGAAGCCATTTTATGCTATTTTGCATAGAATTGATGTGGGGATTGTGATTGATTTGGAGCCTGCTAGGTCTGGTGATCCTGCATTGTCACTTGCAGGAGCTGTGCAGTCTCAGAAACTCGCGGTTCGCGCAATTTCTAGGCTTCAGTCTCTCCCTGGAGGGGACATTGGCGTGTTGTGTGATACTGTTGTGGAGGATGTTCAGAAGCTTACTGGATATGACAGAGTTATGGTTTATAAGTTCCATGACGATGATCACGGTGAGGTTGTGTCTGAAATCAGAAGGTCGGATTTGGAGTCTTATTTGGGTTTGCATTATCCTGCCACGGATATCCCCCAAGCTGCCCGTTTCTTGTTCAAGCAGAATCGCGTGCGTATGATCTGTGATTGCAATGTGAATCCGGTTAAAGTCATTCAAAGTGAAGAACTAAAGCAGCCTTTGTGCTTGGTCAATTCAACCCTCCGGTCTCCACATGGCTGCCACAGACAGTACATGGCGAATATGGGCTCGATTGCCTCACTGGTGATGGCGGTTATCATCAATGGTAATGATTCAACAAAGCTTTGGGGGTTGGTGGTGTGCCACCACACTTCCCCCCGCTATGTCCCTTTCCCTCTTCGCTATGCTTGTCAGTTTCTTATGCAGGCATTTGGGCTGCAAATCTGCATGGAGCTTCAATTGGCTTCGCAGTTGGCTGAGAAAAAGATTCTCAGAACACAAACCTTGCTTTGTGACATGCTCCTCCGGGACTCCCCATCTGGTATTGTGACCCAATCTCCGAGTATAATGGATCTCGTGAAGTGTGACGGAGCTGCATTTTACTATGGCGGGACATGTTGGTTGATGGGGGTAACTCCAACCGAATCACAGGTTAAAGATATAGCAGAGTGGCTGCTAAAAAATCATGGGGATTCCACAGGTCTGAGTACTGATAGTTTGGCGGAAGTTGGTTACCCTGGTGCACCTTTACTGGGTAATGCAGTTTGTGGTATGGCTACCGCAAGAGTCAGTTCAAAAGATTTCTTGTTTTGGTTCAGGTCGCACACGGCAGAGCAAGTCAACTGGGGAGGAGCTAAGCATCATCCAGAGGATAAGGATGACGGTGGAAAGATGCACCCGAGATCATCATTTAAAGCTTTTATTGAAATAGTAAAAAGTAGAAGTTTGCCTTGGGAGATCTCTGAGATTAATGCCATCCACTCTTTACAGCTCATAATGAGAGACTCGTTTCAGGATATCGAGGAAACTGGTTCAAAGGCAGTACAACAGAGTGATGCTGAGATGCAGTTGCAGGAGATAGATGAACTCAGTTCCGCGGCATGTGAAATGGTTAAATTGATCGAGACAGCTTCAGTTCCGATTTTTGGGGTTGATTCGAATGGTCTCATCAATGGATGGAATGCAAAAATAGCTGAGTTGACAGGACTACAAGATAGCGAAGCTATGGGCAAGTCCCTTGTTGACGAAATTGTTTATGAGGACTCGCGTGAAGCGGTTGAAAATCTTTTACGTAGAGCGTTACAAGGTGAGGAGGACAAGAACATTGAGTTAAAATTGAGAAATTTTGGGCTTCCACAACATAAGTCGGTTGTCTATATTGTGGCCAACACTTGCATGAGTAGGAGTCGTGCAAAGGATGTTGTCGGTGTATGCTTCGTGGGTCAGGATATTACTTGTGAAAAGGTTGTTATGGATAAATTCATCCGCTTGCAAGGGGATTACAAGGCCATCATACAGAGTCTTAACCCCTTGATTCCACCGATATTTGCATCTGATGAGAAAGCATGTTGCTCAGAATGGAATGCAGCCATGGAAACGCTGACTGGTTGGACTCGAGAAGACGTTATTGGTAAAACGCTTATTGGGGAAATCTTTGGAGGTTTCTGTCAACTGAAAGGTCAATATACGCTGACTAAGTTTATGATTATGTTGTACCAAGGAATCAGTGGCACAGAGATTGAAAAGTTTCCATTCGGGTTTTTTGATAGAAAGGGTAATTTTGTTGAGGTGCTCTTAACTGCTAGCAAGAGGACTGATGCAGGTGGGAATATAATCGGTTGTTTTTGTTTCTTGCAAATTTTTTTGCGTGACATGCAGCCGGCCTTGGAAGGACACAAACAAGAGGATACAGAATACTTTTCGAAACTTAAGCAATTAACATACATGCGGCAAGAGATGAAAAATCCTTTAAATGGCATTCGGTTTACCCACAGACTCCTTGAAAGTACAACTATTTCAGAAAATCAGAAGCAATTTCTTGACACTAGCGATGCATGTGAAAGACAAATCATGGCGATCATCGAGGATATGGATGTGAGAAGCGTAGCCGAAGGTAGCGCGAAGCTGAACATGGAAGAATTTATGTTGGGAAATGTTTTGGATGTCATCATTTGCCAATCAATGCTCTCGCTGAGGGAAAAGAACTTGCAGCTCCTTCATGAGATTCCAGAAGAGATCAAATCACTATCTTTGCAAGGCGATCAAATCCGGCTTCAGTTGGTTTTGTCAGATTTCTTGCTCAATGTAGTGAGTCATGCACCTTCCCCAAATGGATGGGTCGAAATCAAAATTTCACCTGGTCTGAAGTTAATACAGGACAACAACAATTATATCCGTCTCCAGATCAGAATGACACACCCCGGCCAAGGCCTTCCGGCGGTGCTCATCCAGGATATGTTCGAGGGTGGAAACAGATGGACGACACAAGAAGGGTTCGGGCTAAATCTGTCGCGGAAACTTCTGAATAGAATGAATGGTAAGGTGCAGTATGTTAGAGAGCACAATAAATGTTACTTTCTCGTTGACATCGAACTTCAGATGAAGAAAGAGAGGCAGAGGCTGCCTGTGTCTGAAGCTGATATGAGCACAACGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1133

Amino Acids

126.2

Weight (kDa)

5.76

Isoelectric Point (pI)

53.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 80 - 196 2.6e-33 PAS fold
GAF PF01590 230 - 398 4.2e-18 GAF domain
PHY PF00360 412 - 585 2.1e-56 Phytochrome region
PAS PF00989 616 - 731 1.4e-25 PAS fold
PAS_4 PF08448 624 - 732 8.2e-08 PAS fold
PAS PF00989 747 - 866 3.4e-18 PAS fold
HisKA PF00512 889 - 944 1.3e-10 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1000 - 1101 1.2e-11 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AarI CACCTGC 1 cut(s) 2552
Acc36I ACCTGC 1 cut(s) 2552
AccIII TCCGGA 1 cut(s) 208
AclWI GGATC 2 cut(s) 593, 1320
AcoI YGGCCR 4 cut(s) 87, 141, 2124, 3163
AcsI RAATTY 5 cut(s) 2083, 2219, 2598, 2879, 3090
AcuI CTGAAG 6 cut(s) 650, 1857, 2999, 3125, 3320, 3393
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 7 cut(s) 149, 172, 1013, 1450, 1785, 2456, 2749
AfiI CCNNNNNNNGG 7 cut(s) 502, 682, 1493, 1886, 2714, 3167, 3178
AflII CTTAAG 1 cut(s) 2669
AflIII ACRYGT 1 cut(s) 1355
AjnI CCWGG 4 cut(s) 676, 1477, 3097, 3189
AjuI GAANNNNNNNTTGG 4 cut(s) 1675, 1707, 2352, 2384
Alw21I GWGCWC 6 cut(s) 521, 1486, 2535, 3186, 3303, 3389
Alw26I GTCTC 8 cut(s) 169, 636, 675, 993, 1737, 1859, 1910, 3143
Alw44I GTGCAC 1 cut(s) 1482
AlwI GGATC 2 cut(s) 593, 1320
AlwNI CAGNNNCTG 2 cut(s) 623, 3361
Ama87I CYCGRG 3 cut(s) 221, 1639, 2355
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 7 cut(s) 87, 141, 2124, 2247, 2622, 3163, 3169
ApaLI GTGCAC 1 cut(s) 1482
ApoI RAATTY 5 cut(s) 2083, 2219, 2598, 2879, 3090
AseI ATTAAT 1 cut(s) 1710
Asp700I GAANNNNTTC 1 cut(s) 2487
AspLEI GCGC 3 cut(s) 35, 653, 2861
AsuC2I CCSGG 3 cut(s) 497, 1268, 3162
AsuHPI GGTGA 5 cut(s) 608, 794, 1057, 2060, 3087
AsuII TTCGAA 3 cut(s) 316, 1898, 2663
AsuNHI GCTAGC 1 cut(s) 2543
AvaI CYCGRG 3 cut(s) 221, 1639, 2355
BaeGI GKGCMC 1 cut(s) 1486
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 2126
BanII GRGCYC 1 cut(s) 1031
BauI CACGAG 1 cut(s) 1316
BbsI GAAGAC 1 cut(s) 2367
Bbv12I GWGCWC 6 cut(s) 521, 1486, 2535, 3186, 3303, 3389
BbvCI CCTCAGC 1 cut(s) 2933
BccI CCATC 8 cut(s) 1042, 1285, 1360, 1724, 1911, 2258, 3069, 3210
BceAI ACGGC 2 cut(s) 146, 1584
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 4 cut(s) 678, 1479, 3099, 3191
BciVI GTATCC 1 cut(s) 2641
BclI TGATCA 1 cut(s) 775
BcnI CCSGG 3 cut(s) 497, 1268, 3162
BcoDI GTCTC 8 cut(s) 169, 636, 675, 993, 1737, 1859, 1910, 3143
BfaI CTAG 5 cut(s) 513, 588, 660, 2544, 2787
BfmI CTRYAG 2 cut(s) 118, 418
BfrI CTTAAG 1 cut(s) 2669
BfuAI ACCTGC 1 cut(s) 2552
BfuI GTATCC 1 cut(s) 2641
BglII AGATCT 1 cut(s) 1698
BlpI GCTNAGC 1 cut(s) 1596
BmcAI AGTACT 1 cut(s) 1450
Bme1390I CCNGG 7 cut(s) 497, 678, 1268, 1479, 3099, 3162, 3191
BmeT110I CYCGRG 3 cut(s) 221, 1639, 2355
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 7 cut(s) 497, 678, 1268, 1479, 3099, 3162, 3191
BmrI ACTGGG 2 cut(s) 1502, 1594
BmsI GCATC 7 cut(s) 1609, 1623, 1786, 1794, 2300, 2548, 2782
BmtI GCTAGC 1 cut(s) 2547
BmuI ACTGGG 2 cut(s) 1502, 1594
BpiI GAAGAC 1 cut(s) 2367
BpmI CTGGAG 2 cut(s) 699, 3125
Bpu10I CCTNAGC 1 cut(s) 2933
Bpu1102I GCTNAGC 1 cut(s) 1596
Bpu14I TTCGAA 3 cut(s) 316, 1898, 2663
BpuMI CCSGG 3 cut(s) 497, 1268, 3162
BsaAI YACGTR 1 cut(s) 2033
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 2 cut(s) 993, 1910
BsaWI WCCGGW 4 cut(s) 167, 208, 922, 984
BsaXI ACNNNNNCTCC 2 cut(s) 1282, 1312
Bsc4I CCNNNNNNNGG 7 cut(s) 502, 682, 1493, 1886, 2714, 3167, 3178
Bse118I RCCGGY 1 cut(s) 2620
Bse1I ACTGG 6 cut(s) 739, 1047, 1497, 1589, 1774, 2350
Bse3DI GCAATG 1 cut(s) 919
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 4 cut(s) 678, 1479, 3099, 3191
BseLI CCNNNNNNNGG 7 cut(s) 502, 682, 1493, 1886, 2714, 3167, 3178
BseMI GCAATG 1 cut(s) 919
BseNI ACTGG 6 cut(s) 739, 1047, 1497, 1589, 1774, 2350
BseRI GAGGAG 7 cut(s) 37, 40, 43, 239, 1253, 1605, 2066
BseSI GKGCMC 1 cut(s) 1486
BseX3I CGGCCG 1 cut(s) 87
BseYI CCCAGC 1 cut(s) 352
BsgI GTGCAG 2 cut(s) 647, 3305
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 7 cut(s) 89, 143, 2126, 2249, 2624, 3165, 3171
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 6 cut(s) 521, 1486, 2535, 3186, 3303, 3389
BsiHKCI CYCGRG 3 cut(s) 221, 1639, 2355
BslFI GGGAC 5 cut(s) 698, 1112, 1283, 1366, 1960
BslI CCNNNNNNNGG 7 cut(s) 502, 682, 1493, 1886, 2714, 3167, 3178
BsmAI GTCTC 8 cut(s) 169, 636, 675, 993, 1737, 1859, 1910, 3143
BsmBI CGTCTC 2 cut(s) 169, 3143
BsmFI GGGAC 5 cut(s) 698, 1112, 1283, 1366, 1960
BsmI GAATGC 4 cut(s) 41, 1927, 2329, 2718
BsnI GGCC 7 cut(s) 89, 143, 2126, 2249, 2624, 3165, 3171
Bso31I GGTCTC 2 cut(s) 993, 1910
BsoBI CYCGRG 3 cut(s) 221, 1639, 2355
Bsp119I TTCGAA 3 cut(s) 316, 1898, 2663
Bsp1286I GDGCHC 7 cut(s) 521, 1031, 1486, 2535, 3186, 3303, 3389
Bsp13I TCCGGA 1 cut(s) 208
Bsp1720I GCTNAGC 1 cut(s) 1596
Bsp19I CCATGG 1 cut(s) 2331
BspANI GGCC 7 cut(s) 89, 143, 2126, 2249, 2624, 3165, 3171
BspEI TCCGGA 1 cut(s) 208
BspHI TCATGA 1 cut(s) 2959
BspLI GGNNCC 1 cut(s) 582
BspMI ACCTGC 1 cut(s) 2552
BspOI GCTAGC 1 cut(s) 2547
BspPI GGATC 2 cut(s) 593, 1320
BspT104I TTCGAA 3 cut(s) 316, 1898, 2663
BspTI CTTAAG 1 cut(s) 2669
BspTNI GGTCTC 2 cut(s) 993, 1910
BsrDI GCAATG 1 cut(s) 919
BsrFI RCCGGY 1 cut(s) 2620
BsrI ACTGG 6 cut(s) 739, 1047, 1497, 1589, 1774, 2350
BssAI RCCGGY 1 cut(s) 2620
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 1 cut(s) 1316
BssT1I CCWWGG 5 cut(s) 1691, 2331, 2458, 2625, 3166
Bst2BI CACGAG 1 cut(s) 1316
Bst2UI CCWGG 4 cut(s) 678, 1479, 3099, 3191
Bst4CI ACNGT 5 cut(s) 419, 709, 782, 1011, 1625
Bst6I CTCTTC 2 cut(s) 1143, 2967
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 2669
BstAPI GCANNNNNTGC 1 cut(s) 3361
BstBAI YACGTR 1 cut(s) 2033
BstBI TTCGAA 3 cut(s) 316, 1898, 2663
BstC8I GCNNGC 7 cut(s) 294, 585, 893, 1167, 2231, 2545, 2622
BstDSI CCRYGG 5 cut(s) 84, 444, 846, 1836, 2331
BstEII GGTNACC 1 cut(s) 1472
BstHHI GCGC 3 cut(s) 35, 653, 2861
BstMAI GTCTC 8 cut(s) 169, 636, 675, 993, 1737, 1859, 1910, 3143
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 6 cut(s) 455, 464, 650, 1931, 2614, 3361
BstNI CCWGG 4 cut(s) 678, 1479, 3099, 3191
BstNSI RCATGY 7 cut(s) 1261, 1359, 1845, 2310, 2617, 2689, 2799
BstPI GGTNACC 1 cut(s) 1472
BstSCI CCNGG 7 cut(s) 495, 676, 1266, 1477, 3097, 3160, 3189
BstSFI CTRYAG 2 cut(s) 118, 418
BstSLI GKGCMC 1 cut(s) 1486
BstSNI TACGTA 1 cut(s) 2033
BstV2I GAAGAC 1 cut(s) 2367
BstX2I RGATCY 2 cut(s) 1312, 1698
BstXI CCANNNNNNTGG 3 cut(s) 139, 281, 3075
BstYI RGATCY 2 cut(s) 1312, 1698
BstZI CGGCCG 1 cut(s) 87
BsuI GTATCC 1 cut(s) 2641
BsuRI GGCC 7 cut(s) 89, 143, 2126, 2249, 2624, 3165, 3171
BtgI CCRYGG 5 cut(s) 84, 444, 846, 1836, 2331
BtgZI GCGATG 1 cut(s) 2805
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 4 cut(s) 25, 201, 1040, 2473
BveI ACCTGC 1 cut(s) 2552
Cac8I GCNNGC 7 cut(s) 294, 585, 893, 1167, 2231, 2545, 2622
CaiI CAGNNNCTG 2 cut(s) 623, 3361
CciI TCATGA 1 cut(s) 2959
CciNI GCGGCCGC 1 cut(s) 87
CfoI GCGC 3 cut(s) 35, 653, 2861
Cfr10I RCCGGY 1 cut(s) 2620
Cfr42I CCGCGG 3 cut(s) 87, 447, 1839
CseI GACGC 1 cut(s) 137
CsiI ACCWGGT 1 cut(s) 3097
Csp6I GTAC 7 cut(s) 148, 171, 1012, 1449, 1784, 2455, 2748
CviQI GTAC 7 cut(s) 148, 171, 1012, 1449, 1784, 2455, 2748
DraI TTTAAA 2 cut(s) 1654, 2712
EaeI YGGCCR 4 cut(s) 87, 141, 2124, 3163
EagI CGGCCG 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 1143, 2967
EarI CTCTTC 2 cut(s) 1143, 2967
EciI GGCGGA 2 cut(s) 46, 1478
EclXI CGGCCG 1 cut(s) 87
Eco105I TACGTA 1 cut(s) 2033
Eco130I CCWWGG 5 cut(s) 1691, 2331, 2458, 2625, 3166
Eco147I AGGCCT 1 cut(s) 3171
Eco24I GRGCYC 1 cut(s) 1031
Eco31I GGTCTC 2 cut(s) 993, 1910
Eco32I GATATC 2 cut(s) 853, 1759
Eco52I CGGCCG 1 cut(s) 87
Eco57I CTGAAG 6 cut(s) 650, 1857, 2999, 3125, 3320, 3393
Eco88I CYCGRG 3 cut(s) 221, 1639, 2355
Eco91I GGTNACC 1 cut(s) 1472
EcoO65I GGTNACC 1 cut(s) 1472
EcoRII CCWGG 4 cut(s) 676, 1477, 3097, 3189
EcoRV GATATC 2 cut(s) 853, 1759
EcoT14I CCWWGG 5 cut(s) 1691, 2331, 2458, 2625, 3166
EcoT22I ATGCAT 1 cut(s) 2797
EcoT38I GRGCYC 1 cut(s) 1031
ErhI CCWWGG 5 cut(s) 1691, 2331, 2458, 2625, 3166
Esp3I CGTCTC 2 cut(s) 169, 3143
FalI AAGNNNNNCTT 2 cut(s) 1675, 1707
FaqI GGGAC 5 cut(s) 698, 1112, 1283, 1366, 1960
FauI CCCGC 3 cut(s) 91, 1127, 1343
FbaI TGATCA 1 cut(s) 775
FriOI GRGCYC 1 cut(s) 1031
FspBI CTAG 5 cut(s) 513, 588, 660, 2544, 2787
GlaI GCGC 3 cut(s) 34, 652, 2860
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 699, 3125
HaeIII GGCC 7 cut(s) 89, 143, 2126, 2249, 2624, 3165, 3171
HgaI GACGC 1 cut(s) 137
HhaI GCGC 3 cut(s) 35, 653, 2861
Hin1I GRCGYC 1 cut(s) 129
Hin6I GCGC 3 cut(s) 33, 651, 2859
HinP1I GCGC 3 cut(s) 33, 651, 2859
HincII GTYRAC 5 cut(s) 1582, 1941, 1984, 2411, 3325
HindII GTYRAC 5 cut(s) 1582, 1941, 1984, 2411, 3325
HindIII AAGCTT 3 cut(s) 728, 1082, 1656
HphI GGTGA 5 cut(s) 608, 794, 1057, 2060, 3087
Hpy166II GTNNAC 8 cut(s) 432, 1484, 1582, 1941, 1984, 2411, 2727, 3325
Hpy8I GTNNAC 8 cut(s) 432, 1484, 1582, 1941, 1984, 2411, 2727, 3325
HpyAV CCTTC 9 cut(s) 187, 447, 798, 2624, 2846, 2966, 3072, 3182, 3224
HpyCH4III ACNGT 5 cut(s) 419, 709, 782, 1011, 1625
HpyCH4IV ACGT 2 cut(s) 2032, 2364
HpyF10VI GCNNNNNNNGC 6 cut(s) 455, 464, 650, 1931, 2614, 3361
HpySE526I ACGT 2 cut(s) 2032, 2364
Hsp92I GRCGYC 1 cut(s) 129
HspAI GCGC 3 cut(s) 33, 651, 2859
Kpn2I TCCGGA 1 cut(s) 208
KroI GCCGGC 1 cut(s) 2620
KroNI GCCGGC 1 cut(s) 2622
Ksp22I TGATCA 1 cut(s) 775
KspI CCGCGG 3 cut(s) 87, 447, 1839
LweI GCATC 7 cut(s) 1609, 1623, 1786, 1794, 2300, 2548, 2782
MabI ACCWGGT 1 cut(s) 3097
MaeI CTAG 5 cut(s) 513, 588, 660, 2544, 2787
MaeII ACGT 2 cut(s) 2032, 2364
MboII GAAGA 7 cut(s) 432, 956, 1130, 2372, 2888, 2984, 3355
MfeI CAATTG 1 cut(s) 1199
MflI RGATCY 2 cut(s) 1312, 1698
MhlI GDGCHC 7 cut(s) 521, 1031, 1486, 2535, 3186, 3303, 3389
MlsI TGGCCA 1 cut(s) 2126
MluNI TGGCCA 1 cut(s) 2126
MmeI TCCRAC 3 cut(s) 789, 1403, 2330
Mox20I TGGCCA 1 cut(s) 2126
Mph1103I ATGCAT 1 cut(s) 2797
MroI TCCGGA 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 2620
MroXI GAANNNNTTC 1 cut(s) 2487
MscI TGGCCA 1 cut(s) 2126
MslI CAYNNNNRTG 3 cut(s) 692, 771, 2135
Msp20I TGGCCA 1 cut(s) 2126
MspA1I CMGCKG 3 cut(s) 86, 446, 1838
MspCI CTTAAG 1 cut(s) 2669
MspR9I CCNGG 7 cut(s) 497, 678, 1268, 1479, 3099, 3162, 3191
MunI CAATTG 1 cut(s) 1199
Mva1269I GAATGC 4 cut(s) 41, 1927, 2329, 2718
MvaI CCWGG 4 cut(s) 678, 1479, 3099, 3191
MwoI GCNNNNNNNGC 6 cut(s) 455, 464, 650, 1931, 2614, 3361
NaeI GCCGGC 1 cut(s) 2622
NciI CCSGG 3 cut(s) 497, 1268, 3162
NcoI CCATGG 1 cut(s) 2331
NgoMIV GCCGGC 1 cut(s) 2620
NheI GCTAGC 1 cut(s) 2543
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 1 cut(s) 169
NmuCI GTSAC 6 cut(s) 20, 609, 1253, 1288, 1325, 2609
NotI GCGGCCGC 1 cut(s) 87
NsiI ATGCAT 1 cut(s) 2797
NspI RCATGY 7 cut(s) 1261, 1359, 1845, 2310, 2617, 2689, 2799
NspV TTCGAA 3 cut(s) 316, 1898, 2663
PaeR7I CTCGAG 1 cut(s) 2355
PagI TCATGA 1 cut(s) 2959
PaqCI CACCTGC 1 cut(s) 2552
PceI AGGCCT 1 cut(s) 3171
PciI ACATGT 1 cut(s) 1355
PcsI WCGNNNNNNNCGW 1 cut(s) 3327
PctI GAATGC 4 cut(s) 41, 1927, 2329, 2718
PdiI GCCGGC 1 cut(s) 2622
PdmI GAANNNNTTC 1 cut(s) 2487
PfoI TCCNGGA 2 cut(s) 1266, 3189
Ppu21I YACGTR 1 cut(s) 2033
PscI ACATGT 1 cut(s) 1355
PshBI ATTAAT 1 cut(s) 1710
PsiI TTATAA 1 cut(s) 759
Psp6I CCWGG 4 cut(s) 676, 1477, 3097, 3189
PspEI GGTNACC 1 cut(s) 1472
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 4 cut(s) 676, 1477, 3097, 3189
PspN4I GGNNCC 1 cut(s) 582
PsrI GAACNNNNNNTAC 2 cut(s) 415, 447
PstNI CAGNNNCTG 2 cut(s) 623, 3361
PsuI RGATCY 2 cut(s) 1312, 1698
RsaI GTAC 7 cut(s) 149, 172, 1013, 1450, 1785, 2456, 2749
RsaNI GTAC 7 cut(s) 148, 171, 1012, 1449, 1784, 2455, 2748
RseI CAYNNNNRTG 3 cut(s) 692, 771, 2135
SacII CCGCGG 3 cut(s) 87, 447, 1839
ScaI AGTACT 1 cut(s) 1450
ScrFI CCNGG 7 cut(s) 497, 678, 1268, 1479, 3099, 3162, 3191
SduI GDGCHC 7 cut(s) 521, 1031, 1486, 2535, 3186, 3303, 3389
SexAI ACCWGGT 1 cut(s) 3097
SfaNI GCATC 7 cut(s) 1609, 1623, 1786, 1794, 2300, 2548, 2782
SfcI CTRYAG 2 cut(s) 118, 418
Sfr274I CTCGAG 1 cut(s) 2355
Sfr303I CCGCGG 3 cut(s) 87, 447, 1839
SfuI TTCGAA 3 cut(s) 316, 1898, 2663
SgrBI CCGCGG 3 cut(s) 87, 447, 1839
SlaI CTCGAG 1 cut(s) 2355
SmiMI CAYNNNNRTG 3 cut(s) 692, 771, 2135
SmlI CTYRAG 2 cut(s) 2355, 2669
SmoI CTYRAG 2 cut(s) 2355, 2669
SnaBI TACGTA 1 cut(s) 2033
SseBI AGGCCT 1 cut(s) 3171
SspMI CTAG 5 cut(s) 513, 588, 660, 2544, 2787
StuI AGGCCT 1 cut(s) 3171
StyD4I CCNGG 7 cut(s) 495, 676, 1266, 1477, 3097, 3160, 3189
StyI CCWWGG 5 cut(s) 1691, 2331, 2458, 2625, 3166
TaaI ACNGT 5 cut(s) 419, 709, 782, 1011, 1625
TaiI ACGT 2 cut(s) 2035, 2367
TatI WGTACW 4 cut(s) 1011, 1448, 1783, 2747
TauI GCSGC 7 cut(s) 38, 41, 89, 92, 449, 1841, 2692
TscAI CASTG 4 cut(s) 25, 201, 1047, 2473
TseFI GTSAC 6 cut(s) 20, 609, 1253, 1288, 1325, 2609
Tsp45I GTSAC 6 cut(s) 20, 609, 1253, 1288, 1325, 2609
TspDTI ATGAA 7 cut(s) 86, 1839, 2212, 2714, 2948, 3287, 3356
TspGWI ACGGA 3 cut(s) 863, 1344, 3125
TspRI CASTG 4 cut(s) 25, 201, 1047, 2473
Vha464I CTTAAG 1 cut(s) 2669
VneI GTGCAC 1 cut(s) 1482
VspI ATTAAT 1 cut(s) 1710
XapI RAATTY 5 cut(s) 2083, 2219, 2598, 2879, 3090
XceI RCATGY 7 cut(s) 1261, 1359, 1845, 2310, 2617, 2689, 2799
XcmI CCANNNNNNNNNTGG 1 cut(s) 2465
XhoI CTCGAG 1 cut(s) 2355
XmnI GAANNNNTTC 1 cut(s) 2487
XspI CTAG 5 cut(s) 513, 588, 660, 2544, 2787
ZrmI AGTACT 1 cut(s) 1450
Zsp2I ATGCAT 1 cut(s) 2797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.