RchiOBHm_Chr5g0012921

Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0007664
Physical Location & Seq
Reverse (-)
8772441 .. 8776897
4457 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29347

Sequence Viewer

Length: 3447 bp
ATGTCCAAATTACTTGGGAAGCGTTTCAACAATAAAAACATGGAGCCTGAGAGAACCACCGGTACATTCTCATCCTCAGCTACAAGCAACGTGAGGCCCAACAACAACACCATGATCACCGTCAAAAGAGATAAAGCAATTATTGCTCACTACAATGCAGACGCTGGACTTTTAAATGAGTTTGAACAGTCCACTGTGTCTGGTAAGTCCTTTAACTATACAAGATCAATGCTTCATCCACTTCAGTCTGTCCCTGAGGAGCACATAACTGCTTATTTCTCAAAAATCCAAAGAGGCGCTCTTGTTCAGTCTTTTGGGTGCATGCTTGCAATTGATGAACCTACGTTTAGGATTATCTGTTATAGTGAGAACTGCTCTGAGTTTTTGGGTTTGGATGGTATTTTTGAGTCAAAAGAAGAAAAGGGTCTGATTGGAATTGATGCTAAAACTCTGTTTTCACCCTCATCTGGGGCTTCACTGGCCAAAGCGGCCGCTTCGACGGAGCTATCATTGCTGAACCCAATTTGGGTGTATTCTAGGACCACCCAAAAGCCATTTTATGCTATATTGCATAGGATTGATGTTGGGATTGTGATTGATTTAGAGCCTGCCAAGTGTGGTGATCCTGCATTGTCCCTTGCAGGGGCTGTGCAGTCCCAGAAGCTTGCGGTTCGGGCAATCTCGAGGCTTCAGTCTCTCCCTGGAGGAGATATTGGGACTCTGTGTGACACTGTTGCGGAGGATGTTCAGAAGCTGACCGGATATGACAGAGTTATGGTGTACAAGTTTCATGAAGATGATCATGGGGAAGTTGTGTCTGAGATCAGAAGGTCGGATTTGGAGTCCTATTTGGGGTTGCATTATCCTGCCACGGATATCCCTCAGGCGGCTCGGTTCTTGTTCAAGCAGAATCGGGTGCGCATGATTTGTGATTGCAATGCAAATCCTGTAAAACTCATTCAAAGTGAAGAGCTGGAGCAGCCTCTGTGCTTGGTGAATTCAACTCTCCGGTCACCACACGATTGCCACAGAACATACATGTTAAACATGGGCTCAGTTGCATCACTGGTCATGGCGGTTACTATCAATAGTCCTGATTCCACCAAGCTTTGGGGGTTGGTGGTGTGCCATCACACTTCACCCCGCTATGTTCCTTTCCCTCTTCGCTATGCTTGTGAATTTCTTATGCAGGCATTTGTGTTGCAGCTTAACATGGAGCTTCAGTTGGCATTACAATTGGCCGAAAAGAAGATTCTCAAAACACAGACTTTACTTTGTGACATGCTCCTCCGGGATGCCCCGTCTGGTATTGTAACCCAATCTCCTAGTATAATGGATCTTGTCAAGTCTGATGGGGCTGCATTGTACTATAGTGGGACGTGTTGGTTGACGGGTGTCACACCAACTGAGTCACAGGTTAAAGATATAGCCGAGTGGCTGCTGAAGAATCATGGGGATTCTACAGGACTGAGTACTGACAGTTTGGCTGAAGCTGGTTACCCTGCTGCAGCTCTACTAGGCAATGCGGTTTGTGGTATGGCTACTGCAAGAATCAGTTCAAACGATTTTCTGTTTTGGTTCAGGTCTCACACCGCCGAGGAAGTCAAGTGGGGAGGAGCTAAGCATCATCCACAGGATAAGGATGATGGTGGAAGAATGCACCCAAGATCATCATTTAAGGCTTTTCTTGAAGTAGCGAAAAGTAGAAGCTTGCCTTGGGAGGACTCCGAGATTAATGCCATCCACTCTTTACAGCTCATAATGAGAGACTCGTTCCAGGATATGGAGGAAACTGGTCCTAAGCCAATAAATAATAATGCTCGTCCTGGTGATGCTGAGATGCAGGTGGAGGGGATGGATGAGATCAGTTCTGTGGCATGTGAAATGGTTAAATTGATTGAGACGGCTCAAGTTCCGATTTTCGGGGTTGATTCAGAGGGTCTCATCAATGGATGGAATGCAAAGATGGCTGAATTGACAGGATTACAAGATGGTGAAGCAATGGGGAAGTCCCTTGTTAATGATATTGTTCATGAGGACTCGCGTGGAGCTGTTGAAAGTATCTTATCCAGAGCGTTACAAGGTGATGAGGACAAGAATATTGAGTTGAAACTGAGAAATTTTGGACTTGCTCAGCAAAACTCAGTGGTATATATCGTGGCCAACAGTTGCACAAGTAGGAATCATGCAAAGAATGTTGTTGGCGCATGCTTCGTGGGTCAAGACATTACTTGTGAAAAACTTGTTACGGATAAATTTATCCAGTTGCAAGGGGACTATAGGGCTATCATACAGAGTCCTAGCCCATTGATTCCACCAATATTTGCATCTGATGAGAATGCCTACTGCTCTGAATGGAATGCAGCAATGGAAAAGCTGACTGGTTTGACAAGAGATGATGTTATTGGTAAAATGCTCCCTGGAGAAATCTTTGGAGAGTTCTGTCAATTGAAAGGTCAAGATTCGCTAACCAAATTTAGGATTGTACTATATCAAGGAATCATTGGGCAAGACATTGAAAAGTTTCCATTTGGGTTTTTTGATAGAGAAGGGAAGTATGTTGAGGTGCTCTTAACAGCAAGCAAGAGGACTGATGCCAATGGGAATATAGTTGGTTGCTTCTGCTTCTTGCAAACATTTCTACCAGACCTGCAGCCAGCCATGGATGGACTCGGACAAGAGGATAAATTCTCTTCAAAACTTAAGGAATTAACTTACATGCGACAAGAGATGAAAAATCCTTTAAGTGGTATCCGGTTTACACACAGACTCCTTGAAAATACAAATGTTTCAGAATATCAGAAGCAATTTCTTGACACTAGTGATGCATGTGAGAGACAAATCATGGCAATCCTTGAGAATCTGGATACGAGGAGCATAGAGGAAGGCAATGTGAAGCTGAACATGGAAGAATTTCTCTTGAGAACTCTTATAGATGCCATTGTGAGTCAATCAATGATCTCATTGAGGGAAAGAAATCTACAGTTATTTCATGAGATTCCAGAAGAACTCAAAACACTATCTTTGCTTGGCGACCAAATCAGGCTTCAGTTGGTCTTGTCAGATTTCTTGCTCAATGTGGTGCATCATACACCTTCTCCAGATGGCTGGGTTGAAATCAGAATTTCACCAGGTCTAAAGCGAATACATGATGGCAACACCTATATCCGTTTACAGTTCAGAATGACTCACCCTGGTCAAGGAATTCCCACTGCACTTATTCAAGATATGTTCGCCGGTGAAGATCAGTGGAATACACAAGAAGGACTTGGGCTAAACCTGTCCAGAAAACTTCTCGGTAGAATGAATGGTCAGGTCCAGTACGTAAGAGAGCAAGGTAAGTGTTACTTCCTCATTGATATTGAACTTAGAACAAGGAAAGAAAGGCAGAGGGTGTTATTGTCACAATCTGATAGGAGCACGTTACTTAAATTTTCATCGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GAF HATPase_c HisKA PAS PAS_2 PHY

Protein Analysis

1148

Amino Acids

128.14

Weight (kDa)

5.52

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_2 PF08446 92 - 204 1.3e-33 PAS fold
GAF PF01590 237 - 407 4.7e-17 GAF domain
PHY PF00360 420 - 595 1.3e-56 Phytochrome region
PAS PF00989 628 - 743 2.1e-24 PAS fold
PAS_4 PF08448 635 - 744 9.8e-07 PAS fold
PAS PF00989 759 - 878 4.2e-18 PAS fold
HisKA PF00512 900 - 962 1.4e-09 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 1011 - 1119 3.3e-12 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1834
Acc16I TGCGCA 1 cut(s) 920
Acc36I ACCTGC 2 cut(s) 1834, 2658
AccB7I CCANNNNNTGG 2 cut(s) 1110, 1783
AccII CGCG 1 cut(s) 2044
AciI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
AclWI GGATC 2 cut(s) 617, 1344
AcoI YGGCCR 4 cut(s) 480, 489, 1239, 2160
AcuI CTGAAG 6 cut(s) 227, 674, 1205, 1463, 1509, 3032
AfaI GTAC 6 cut(s) 64, 782, 1367, 1474, 2487, 3323
AflII CTTAAG 1 cut(s) 2702
AflIII ACRYGT 2 cut(s) 1038, 1379
AgeI ACCGGT 1 cut(s) 59
AhlI ACTAGT 1 cut(s) 2819
AjiI CACGTC 1 cut(s) 1380
AjnI CCWGG 6 cut(s) 700, 1776, 1825, 2419, 3130, 3193
AjuI GAANNNNNNNTTGG 4 cut(s) 1699, 1731, 3030, 3062
Alw21I GWGCWC 3 cut(s) 264, 2571, 3422
Alw26I GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
AlwI GGATC 2 cut(s) 617, 1344
AlwNI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
Ama87I CYCGRG 1 cut(s) 682
AoxI GGCC 5 cut(s) 95, 480, 489, 1239, 2160
ApeKI GCWGC 8 cut(s) 979, 1204, 1358, 1438, 1505, 1508, 2363, 2653
AseI ATTAAT 1 cut(s) 1734
AsiGI ACCGGT 1 cut(s) 59
Asp700I GAANNNNTTC 4 cut(s) 23, 1555, 2523, 2913
AspLEI GCGC 3 cut(s) 299, 921, 2207
AspS9I GGNCC 4 cut(s) 96, 540, 1796, 3316
AsuC2I CCSGG 1 cut(s) 1292
AvaI CYCGRG 1 cut(s) 682
AvaII GGWCC 3 cut(s) 540, 1796, 3316
AxyI CCTNAGG 2 cut(s) 255, 882
BalI TGGCCA 2 cut(s) 482, 2162
BanII GRGCYC 1 cut(s) 1055
Bbv12I GWGCWC 3 cut(s) 264, 2571, 3422
BbvCI CCTCAGC 1 cut(s) 76
BbvI GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BceAI ACGGC 1 cut(s) 1920
BcgI CGANNNNNNTGC 2 cut(s) 1718, 1752
BciT130I CCWGG 6 cut(s) 702, 1778, 1827, 2421, 3132, 3195
BciVI GTATCC 2 cut(s) 2762, 2860
BclI TGATCA 2 cut(s) 114, 799
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BcuI ACTAGT 1 cut(s) 2819
BfaI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
BfmI CTRYAG 6 cut(s) 1369, 1461, 1506, 2278, 2651, 2981
BfoI RGCGCY 1 cut(s) 300
BfrI CTTAAG 1 cut(s) 2702
BfuAI ACCTGC 2 cut(s) 1834, 2658
BfuI GTATCC 2 cut(s) 2762, 2860
BglI GCCNNNNNGGC 1 cut(s) 488
BlpI GCTNAGC 2 cut(s) 1620, 2133
BmcAI AGTACT 1 cut(s) 1474
Bme1390I CCNGG 7 cut(s) 702, 1292, 1778, 1827, 2421, 3132, 3195
Bme18I GGWCC 3 cut(s) 540, 1796, 3316
BmeT110I CYCGRG 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 1380
BmgT120I GGNCC 4 cut(s) 96, 540, 1796, 3316
BmiI GGNNCC 1 cut(s) 45
BmrFI CCNGG 7 cut(s) 702, 1292, 1778, 1827, 2421, 3132, 3195
BoxI GACNNNNGTC 1 cut(s) 1394
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 4 cut(s) 723, 995, 2442, 3084
Bpu10I CCTNAGC 2 cut(s) 76, 1800
Bpu1102I GCTNAGC 2 cut(s) 1620, 2133
BpuEI CTTGAG 3 cut(s) 1893, 2876, 2941
BpuMI CCSGG 1 cut(s) 1292
BsaAI YACGTR 1 cut(s) 3325
BsaI GGTCTC 2 cut(s) 1590, 1946
BsaJI CCNNGG 7 cut(s) 700, 870, 1596, 1715, 2419, 2661, 3193
BsaWI WCCGGW 4 cut(s) 59, 758, 1008, 2754
BsaXI ACNNNNNCTCC 8 cut(s) 1208, 1238, 1306, 1336, 2754, 2784, 3082, 3112
Bse118I RCCGGY 2 cut(s) 59, 3236
Bse1I ACTGG 6 cut(s) 483, 1071, 1798, 2263, 2386, 3319
Bse21I CCTNAGG 2 cut(s) 255, 882
Bse3DI GCAATG 6 cut(s) 509, 943, 1528, 2007, 2373, 2896
BseBI CCWGG 6 cut(s) 702, 1778, 1827, 2421, 3132, 3195
BseDI CCNNGG 7 cut(s) 700, 870, 1596, 1715, 2419, 2661, 3193
BseMI GCAATG 6 cut(s) 509, 943, 1528, 2007, 2373, 2896
BseNI ACTGG 6 cut(s) 483, 1071, 1798, 2263, 2386, 3319
BseRI GAGGAG 5 cut(s) 272, 720, 1277, 1629, 2887
BseX3I CGGCCG 1 cut(s) 489
BseXI GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BseYI CCCAGC 1 cut(s) 3108
BsgI GTGCAG 2 cut(s) 671, 3198
Bsh1236I CGCG 1 cut(s) 2044
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BshTI ACCGGT 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 492
BsiHKAI GWGCWC 3 cut(s) 264, 2571, 3422
BsiHKCI CYCGRG 1 cut(s) 682
BsiSI CCGG 6 cut(s) 60, 759, 1009, 1291, 2755, 3237
BslFI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
BsmAI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
BsmI GAATGC 4 cut(s) 1662, 1963, 2344, 2365
BsnI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
Bso31I GGTCTC 2 cut(s) 1590, 1946
BsoBI CYCGRG 1 cut(s) 682
Bsp1286I GDGCHC 4 cut(s) 264, 1055, 2571, 3422
Bsp1407I TGTACA 1 cut(s) 780
Bsp1720I GCTNAGC 2 cut(s) 1620, 2133
Bsp19I CCATGG 1 cut(s) 2661
BspACI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
BspANI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BspFNI CGCG 1 cut(s) 2044
BspHI TCATGA 3 cut(s) 790, 2032, 2992
BspLI GGNNCC 1 cut(s) 45
BspMAI CTGCAG 2 cut(s) 1510, 2655
BspMI ACCTGC 2 cut(s) 1834, 2658
BspPI GGATC 2 cut(s) 617, 1344
BspQI GCTCTTC 1 cut(s) 963
BspTI CTTAAG 1 cut(s) 2702
BspTNI GGTCTC 2 cut(s) 1590, 1946
BsrDI GCAATG 6 cut(s) 509, 943, 1528, 2007, 2373, 2896
BsrFI RCCGGY 2 cut(s) 59, 3236
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 6 cut(s) 483, 1071, 1798, 2263, 2386, 3319
BssAI RCCGGY 2 cut(s) 59, 3236
BssECI CCNNGG 7 cut(s) 700, 870, 1596, 1715, 2419, 2661, 3193
BssT1I CCWWGG 2 cut(s) 1715, 2661
Bst2UI CCWGG 6 cut(s) 702, 1778, 1827, 2421, 3132, 3195
Bst4CI ACNGT 8 cut(s) 121, 189, 196, 733, 1481, 2168, 2985, 3177
Bst6I CTCTTC 3 cut(s) 963, 1167, 2698
BstAFI CTTAAG 1 cut(s) 2702
BstAPI GCANNNNNTGC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 1 cut(s) 3325
BstC8I GCNNGC 9 cut(s) 323, 327, 609, 666, 1191, 1712, 2209, 2581, 2658
BstDSI CCRYGG 2 cut(s) 870, 2661
BstEII GGTNACC 2 cut(s) 1011, 1496
BstENI CCTNNNNNAGG 1 cut(s) 3198
BstFNI CGCG 1 cut(s) 2044
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 3 cut(s) 299, 921, 2207
BstMAI GTCTC 6 cut(s) 699, 1590, 1761, 1895, 1946, 2830
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
BstNI CCWGG 6 cut(s) 702, 1778, 1827, 2421, 3132, 3195
BstNSI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
BstPAI GACNNNNGTC 1 cut(s) 1394
BstPI GGTNACC 2 cut(s) 1011, 1496
BstSCI CCNGG 7 cut(s) 700, 1290, 1776, 1825, 2419, 3130, 3193
BstSFI CTRYAG 6 cut(s) 1369, 1461, 1506, 2278, 2651, 2981
BstSNI TACGTA 1 cut(s) 3325
BstUI CGCG 1 cut(s) 2044
BstV1I GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
BstX2I RGATCY 1 cut(s) 1336
BstXI CCANNNNNNTGG 1 cut(s) 3108
BstYI RGATCY 1 cut(s) 1336
BstZI CGGCCG 1 cut(s) 489
Bsu36I CCTNAGG 2 cut(s) 255, 882
BsuI GTATCC 2 cut(s) 2762, 2860
BsuRI GGCC 5 cut(s) 97, 482, 491, 1241, 2162
BtgI CCRYGG 2 cut(s) 870, 2661
BtrI CACGTC 1 cut(s) 1380
BtsI GCAGTG 1 cut(s) 3210
BtsIMutI CAGTG 7 cut(s) 192, 476, 729, 1064, 2151, 3210, 3254
BveI ACCTGC 2 cut(s) 1834, 2658
Cac8I GCNNGC 9 cut(s) 323, 327, 609, 666, 1191, 1712, 2209, 2581, 2658
CaiI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
CciI TCATGA 3 cut(s) 790, 2032, 2992
CciNI GCGGCCGC 1 cut(s) 489
CfoI GCGC 3 cut(s) 299, 921, 2207
Cfr10I RCCGGY 2 cut(s) 59, 3236
Cfr13I GGNCC 4 cut(s) 96, 540, 1796, 3316
CseI GACGC 1 cut(s) 170
CsiI ACCWGGT 1 cut(s) 3130
Csp6I GTAC 6 cut(s) 63, 781, 1366, 1473, 2486, 3322
CspAI ACCGGT 1 cut(s) 59
CviQI GTAC 6 cut(s) 63, 781, 1366, 1473, 2486, 3322
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 4 cut(s) 480, 489, 1239, 2160
EagI CGGCCG 1 cut(s) 489
Eam1104I CTCTTC 3 cut(s) 963, 1167, 2698
EarI CTCTTC 3 cut(s) 963, 1167, 2698
EclXI CGGCCG 1 cut(s) 489
Eco105I TACGTA 1 cut(s) 3325
Eco130I CCWWGG 2 cut(s) 1715, 2661
Eco24I GRGCYC 1 cut(s) 1055
Eco31I GGTCTC 2 cut(s) 1590, 1946
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 3 cut(s) 540, 1796, 3316
Eco52I CGGCCG 1 cut(s) 489
Eco57I CTGAAG 6 cut(s) 227, 674, 1205, 1463, 1509, 3032
Eco81I CCTNAGG 2 cut(s) 255, 882
Eco88I CYCGRG 1 cut(s) 682
Eco91I GGTNACC 2 cut(s) 1011, 1496
EcoNI CCTNNNNNAGG 1 cut(s) 3198
EcoO65I GGTNACC 2 cut(s) 1011, 1496
EcoRI GAATTC 2 cut(s) 997, 3204
EcoRII CCWGG 6 cut(s) 700, 1776, 1825, 2419, 3130, 3193
EcoRV GATATC 1 cut(s) 877
EcoT14I CCWWGG 2 cut(s) 1715, 2661
EcoT22I ATGCAT 1 cut(s) 2830
EcoT38I GRGCYC 1 cut(s) 1055
ErhI CCWWGG 2 cut(s) 1715, 2661
Esp3I CGTCTC 1 cut(s) 1895
FalI AAGNNNNNCTT 6 cut(s) 1699, 1731, 3252, 3284, 3332, 3364
FaqI GGGAC 7 cut(s) 236, 619, 640, 730, 1390, 1996, 2288
FauI CCCGC 1 cut(s) 1151
FbaI TGATCA 2 cut(s) 114, 799
FriOI GRGCYC 1 cut(s) 1055
FspAI RTGCGCAY 1 cut(s) 920
FspBI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
FspI TGCGCA 1 cut(s) 920
GlaI GCGC 3 cut(s) 298, 920, 2206
GsaI CCCAGC 1 cut(s) 3112
GsuI CTGGAG 4 cut(s) 723, 995, 2442, 3084
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 5 cut(s) 97, 482, 491, 1241, 2162
HapII CCGG 6 cut(s) 60, 759, 1009, 1291, 2755, 3237
HgaI GACGC 1 cut(s) 170
HhaI GCGC 3 cut(s) 299, 921, 2207
Hin6I GCGC 3 cut(s) 297, 919, 2205
HinP1I GCGC 3 cut(s) 297, 919, 2205
HincII GTYRAC 1 cut(s) 1389
HindII GTYRAC 1 cut(s) 1389
HindIII AAGCTT 3 cut(s) 662, 1106, 1708
HpaII CCGG 6 cut(s) 60, 759, 1009, 1291, 2755, 3237
Hpy166II GTNNAC 5 cut(s) 192, 781, 1389, 2760, 3173
Hpy8I GTNNAC 5 cut(s) 192, 781, 1389, 2760, 3173
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 5 cut(s) 822, 2543, 2879, 3105, 3257
HpyCH4III ACNGT 8 cut(s) 121, 189, 196, 733, 1481, 2168, 2985, 3177
HpyCH4IV ACGT 5 cut(s) 90, 344, 1379, 3324, 3422
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
HpySE526I ACGT 5 cut(s) 90, 344, 1379, 3324, 3422
HspAI GCGC 3 cut(s) 297, 919, 2205
Ksp22I TGATCA 2 cut(s) 114, 799
LguI GCTCTTC 1 cut(s) 963
Lsp1109I GCAGC 8 cut(s) 991, 1216, 1345, 1425, 1492, 1520, 2375, 2665
MabI ACCWGGT 1 cut(s) 3130
MaeI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
MaeII ACGT 5 cut(s) 90, 344, 1379, 3324, 3422
MfeI CAATTG 3 cut(s) 330, 1235, 2447
MflI RGATCY 1 cut(s) 1336
MhlI GDGCHC 4 cut(s) 264, 1055, 2571, 3422
MlsI TGGCCA 2 cut(s) 482, 2162
MluNI TGGCCA 2 cut(s) 482, 2162
MmeI TCCRAC 1 cut(s) 813
Mox20I TGGCCA 2 cut(s) 482, 2162
Mph1103I ATGCAT 1 cut(s) 2830
MroXI GAANNNNTTC 4 cut(s) 23, 1555, 2523, 2913
MscI TGGCCA 2 cut(s) 482, 2162
MslI CAYNNNNRTG 2 cut(s) 153, 795
Msp20I TGGCCA 2 cut(s) 482, 2162
MspCI CTTAAG 1 cut(s) 2702
MspI CCGG 6 cut(s) 60, 759, 1009, 1291, 2755, 3237
MspR9I CCNGG 7 cut(s) 702, 1292, 1778, 1827, 2421, 3132, 3195
MunI CAATTG 3 cut(s) 330, 1235, 2447
Mva1269I GAATGC 4 cut(s) 1662, 1963, 2344, 2365
MvaI CCWGG 6 cut(s) 702, 1778, 1827, 2421, 3132, 3195
MvnI CGCG 1 cut(s) 2044
MwoI GCNNNNNNNGC 7 cut(s) 143, 479, 488, 511, 674, 979, 1967
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 2661
NlaIV GGNNCC 1 cut(s) 45
NmeAIII GCCGAG 2 cut(s) 1456, 1621
NmuCI GTSAC 6 cut(s) 725, 1011, 1277, 1396, 1410, 3402
NotI GCGGCCGC 1 cut(s) 489
NsbI TGCGCA 1 cut(s) 920
NsiI ATGCAT 1 cut(s) 2830
NspI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
PaeI GCATGC 2 cut(s) 325, 2211
PaeR7I CTCGAG 1 cut(s) 682
PagI TCATGA 3 cut(s) 790, 2032, 2992
PaqCI CACCTGC 1 cut(s) 1834
PciI ACATGT 1 cut(s) 1038
PciSI GCTCTTC 1 cut(s) 963
PctI GAATGC 4 cut(s) 1662, 1963, 2344, 2365
PdmI GAANNNNTTC 4 cut(s) 23, 1555, 2523, 2913
PflMI CCANNNNNTGG 2 cut(s) 1110, 1783
PfoI TCCNGGA 2 cut(s) 1290, 1776
PinAI ACCGGT 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 3325
PscI ACATGT 1 cut(s) 1038
PshAI GACNNNNGTC 1 cut(s) 1394
PshBI ATTAAT 1 cut(s) 1734
Psp6I CCWGG 6 cut(s) 700, 1776, 1825, 2419, 3130, 3193
PspEI GGTNACC 2 cut(s) 1011, 1496
PspFI CCCAGC 1 cut(s) 3108
PspGI CCWGG 6 cut(s) 700, 1776, 1825, 2419, 3130, 3193
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 4 cut(s) 96, 540, 1796, 3316
PsrI GAACNNNNNNTAC 2 cut(s) 46, 78
PstI CTGCAG 2 cut(s) 1510, 2655
PstNI CAGNNNCTG 4 cut(s) 164, 647, 754, 985
PsuI RGATCY 1 cut(s) 1336
RsaI GTAC 6 cut(s) 64, 782, 1367, 1474, 2487, 3323
RsaNI GTAC 6 cut(s) 63, 781, 1366, 1473, 2486, 3322
RseI CAYNNNNRTG 2 cut(s) 153, 795
SapI GCTCTTC 1 cut(s) 963
Sau96I GGNCC 4 cut(s) 96, 540, 1796, 3316
ScaI AGTACT 1 cut(s) 1474
ScrFI CCNGG 7 cut(s) 702, 1292, 1778, 1827, 2421, 3132, 3195
SduI GDGCHC 4 cut(s) 264, 1055, 2571, 3422
SexAI ACCWGGT 1 cut(s) 3130
SfcI CTRYAG 6 cut(s) 1369, 1461, 1506, 2278, 2651, 2981
SfiI GGCCNNNNNGGCC 1 cut(s) 488
Sfr274I CTCGAG 1 cut(s) 682
SgrAI CRCCGGYG 1 cut(s) 3236
SinI GGWCC 3 cut(s) 540, 1796, 3316
SlaI CTCGAG 1 cut(s) 682
SmiMI CAYNNNNRTG 2 cut(s) 153, 795
SmlI CTYRAG 5 cut(s) 682, 1908, 2702, 2855, 2920
SmoI CTYRAG 5 cut(s) 682, 1908, 2702, 2855, 2920
SnaBI TACGTA 1 cut(s) 3325
SpeI ACTAGT 1 cut(s) 2819
SphI GCATGC 2 cut(s) 325, 2211
SsiI CCGC 9 cut(s) 488, 492, 668, 737, 887, 1076, 1144, 1526, 1593
SspI AATATT 2 cut(s) 2101, 2322
SspMI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
StyD4I CCNGG 7 cut(s) 700, 1290, 1776, 1825, 2419, 3130, 3193
StyI CCWWGG 2 cut(s) 1715, 2661
TaaI ACNGT 8 cut(s) 121, 189, 196, 733, 1481, 2168, 2985, 3177
TaiI ACGT 5 cut(s) 93, 347, 1382, 3327, 3425
TaqI TCGA 2 cut(s) 497, 683
TatI WGTACW 4 cut(s) 780, 1365, 1472, 2485
TauI GCSGC 3 cut(s) 491, 494, 890
TscAI CASTG 7 cut(s) 199, 483, 736, 1071, 2151, 3217, 3254
TseFI GTSAC 6 cut(s) 725, 1011, 1277, 1396, 1410, 3402
TseI GCWGC 8 cut(s) 979, 1204, 1358, 1438, 1505, 1508, 2363, 2653
Tsp45I GTSAC 6 cut(s) 725, 1011, 1277, 1396, 1410, 3402
TspDTI ATGAA 9 cut(s) 224, 351, 779, 807, 2021, 2747, 2981, 3320, 3426
TspGWI ACGGA 4 cut(s) 515, 887, 2264, 3158
TspRI CASTG 7 cut(s) 199, 483, 736, 1071, 2151, 3217, 3254
Van91I CCANNNNNTGG 2 cut(s) 1110, 1783
Vha464I CTTAAG 1 cut(s) 2702
VpaK11BI GGWCC 3 cut(s) 540, 1796, 3316
VspI ATTAAT 1 cut(s) 1734
XagI CCTNNNNNAGG 1 cut(s) 3198
XceI RCATGY 7 cut(s) 325, 1042, 1285, 1881, 2211, 2722, 2832
XhoI CTCGAG 1 cut(s) 682
XmnI GAANNNNTTC 4 cut(s) 23, 1555, 2523, 2913
XspI CTAG 5 cut(s) 537, 1326, 1517, 2301, 2820
ZrmI AGTACT 1 cut(s) 1474
Zsp2I ATGCAT 1 cut(s) 2830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.