MD05G1292900.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006276
Physical Location & Seq
Reverse (-)
42436817 .. 42440901
4085 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1292900.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGATTGCCACGAAAACCATGCTGTCGAGCAACAAGCTGAAGATTAAATTTGCCTGCAAAAGAATTGAAGCTGATCCTGGAAAAAAATTTGAAGCTGATACTAATTACTCTAGGAGTCACTCGTGTGACTATGGGCATCATGCCTCAATCAGGGAGGTCAGCGCAATGGCGAAATCTTCACTTCCAGGTTCTAAGAAGCGTGGATCCTCAGGCGATCTTGAGGGTCAGAAAGAGAAGAAGAGGAAGATTGATCGTAGTGTGAAGGTGCAATGTTTGACGATCCTCAAAACACTGATGAGTAAAAAGGAAAGCTGGCCTTTCAATGAACCAGTTGATCCTGTGAAGCTGGGAATCCCAGATTACTTTCGCATCATCTCTTATCCCATGGATTTGGGCACGGTAAAATCAAAGCTGCAGAAAGATTTGTACTTAAGCGCTGAAGAGTTTGCAGCTGATGTCAGGCTGACTTTTTCTAATGCCATGCTCTATAATCCTCCTGGCAATCCTTTTCATCATCTGGCAGAAAACCTGAATAGTTTTTTTGAGATGAGATGGGGTCTTTTAGATGAGAAGTTGAGTCATGGAAGCTCCAAGGTTGAATCAGGGAATCCATCGAATGGGCAAATTAAGAAAGTAACTTGTGCTAGACAGAATCCATGCAACTCTCCTCCCTTGCAAAATAGTTCGGTGAGCAAGAGGCCTATTTCAGGTGAAGAGAAGGTCAGAGTCCCTGTTGGTGCAACTGATGCTGAGGCAGAGCTTTCTAAAACAGCTCAGAATTCTAATCCCAAATTGTCATCAAAGAACTTAAATAGAAGCACCGATAGTGCCTGTAAACAAGCTTCAGGCTCTATTAATGCAAAGCAGCCATCAAGCCTGGTTTGCTTAAAATGTGGCTCATGTGGCAACGTTGCATGTCAATGCAGGCGTCCTAGTGACTCGACCCATAAGTCCTTTAATGACAAATCTTCTGAAAGGTCAATGGGCAGAGAGCATGGTTTGTCTAATTCTACTGTACCCAGACTGGATTGTCAAACAAAAAGCTTGTCAACATCCCAGACGATCAAATCAGATCCAGAATCTGATGGGGCTGTAAGTGCTTTGGATGGTGAAAACATATGTTCCAGTTCTAGGCTTACAACTCCAGTAGTGGATGCTGCTTCTGGAGAAGGATGGAGCACTTCTCTTTTTGATGTTGAACTGTCCCCAAAAAGGGCTCTTCGTGCTGCAATGCTAAAGAGTCGCTTTGCAGACACTATTTGGAAAGCTCAACAGCAGAAACTTCTAGATCATGGTGATAGAAGTGATCCGCTTAAGATGCAGCAAGAAAGGGCTAGACTGGAGAGGAAGCAGCGTGAAGAGAAAGCAAGGATCGAAGCAGAAATAAGAGCTGCTGAAGCTGCCACACGAATGAGGGCTGAGAATGAGTTGAAGCAGCAACGGGAAAGAAAAAGAGAAGAAGCTCGAATTGCACTTGAGAAGATGAGGCAAACAGTTGAGATCGAGACAAGCTTGAAGATCCTAGAGGAACTTGGAAGGTTTGCTGGGTTCTCCGTATCCGCTCCTATTCTCAACTTTAATCGTGGGTCTGGGGCTTTCGGGGGGTCACATCCCCAATCACCTTTGGAGCAGCTAGGTTTATTTCTAAAGCCCGAATATTCAGGAGACGACGACGACGACAACGATGAATCAACGCTGTACGGGGATGGGGAGGAAGGAGAGATCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain

Protein Analysis

577

Amino Acids

63.58

Weight (kDa)

8.84

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 91 - 175 5.1e-20 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 949, 1029
AccB7I CCANNNNNTGG 1 cut(s) 617
AccBSI CCGCTC 1 cut(s) 1562
AciI CCGC 2 cut(s) 1310, 1560
AclI AACGTT 1 cut(s) 909
AclWI GGATC 9 cut(s) 68, 198, 211, 274, 329, 1067, 1301, 1379, 1513
AcsI RAATTY 3 cut(s) 47, 86, 778
AcuI CTGAAG 4 cut(s) 59, 459, 828, 1416
AcyI GRCGYC 1 cut(s) 928
AfaI GTAC 3 cut(s) 428, 1017, 1700
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 6 cut(s) 150, 617, 707, 1131, 1212, 1213
AflII CTTAAG 2 cut(s) 430, 1313
AgsI TTSAA 7 cut(s) 68, 92, 322, 599, 1199, 1432, 1516
AjnI CCWGG 4 cut(s) 76, 184, 496, 876
AleI CACNNNNGTG 1 cut(s) 123
Alw21I GWGCWC 1 cut(s) 1181
Alw26I GTCTC 2 cut(s) 1499, 1659
AlwI GGATC 9 cut(s) 68, 198, 211, 274, 329, 1067, 1301, 1379, 1513
AlwNI CAGNNNCTG 1 cut(s) 1082
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 2 cut(s) 314, 698
ApoI RAATTY 3 cut(s) 47, 86, 778
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
AseI ATTAAT 1 cut(s) 855
AspLEI GCGC 2 cut(s) 164, 437
AsuHPI GGTGA 5 cut(s) 700, 722, 1121, 1307, 1611
AxyI CCTNAGG 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 398
BamHI GGATCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 1219
BarI GAAGNNNNNNTAC 2 cut(s) 826, 858
BauI CACGAG 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 1181
BbvCI CCTCAGC 1 cut(s) 750
BccI CCATC 7 cut(s) 546, 619, 877, 1079, 1100, 1167, 1700
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 4 cut(s) 78, 186, 498, 878
BciVI GTATCC 1 cut(s) 1567
BcoDI GTCTC 2 cut(s) 1499, 1659
BfaI CTAG 8 cut(s) 111, 645, 933, 1131, 1286, 1335, 1523, 1634
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 438
BfrI CTTAAG 2 cut(s) 430, 1313
BfuI GTATCC 1 cut(s) 1567
Bme1390I CCNGG 4 cut(s) 78, 186, 498, 878
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 4 cut(s) 78, 186, 498, 878
BmsI GCATC 5 cut(s) 145, 378, 736, 1144, 1308
BplI GAGNNNNNCTC 2 cut(s) 1168, 1200
BpmI CTGGAG 3 cut(s) 1128, 1185, 1361
Bpu10I CCTNAGC 1 cut(s) 750
BpuEI CTTGAG 2 cut(s) 239, 1496
BsaHI GRCGYC 1 cut(s) 928
BsaJI CCNNGG 2 cut(s) 384, 591
BsaXI ACNNNNNCTCC 1 cut(s) 1710
Bsc4I CCNNNNNNNGG 6 cut(s) 150, 617, 707, 1131, 1212, 1213
Bse1I ACTGG 5 cut(s) 329, 1029, 1125, 1145, 1344
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 3 cut(s) 171, 275, 1236
BseBI CCWGG 4 cut(s) 78, 186, 498, 878
BseDI CCNNGG 2 cut(s) 384, 591
BseGI GGATG 6 cut(s) 1052, 1111, 1159, 1178, 1609, 1711
BseLI CCNNNNNNNGG 6 cut(s) 150, 617, 707, 1131, 1212, 1213
BseMI GCAATG 3 cut(s) 171, 275, 1236
BseMII CTCAG 4 cut(s) 222, 741, 788, 1410
BseNI ACTGG 5 cut(s) 329, 1029, 1125, 1145, 1344
BseRI GAGGAG 1 cut(s) 657
BseSI GKGCMC 1 cut(s) 398
BseYI CCCAGC 2 cut(s) 346, 1544
BshFI GGCC 2 cut(s) 316, 700
BsiHKAI GWGCWC 1 cut(s) 1181
BslFI GGGAC 2 cut(s) 713, 1189
BslI CCNNNNNNNGG 6 cut(s) 150, 617, 707, 1131, 1212, 1213
BsmAI GTCTC 2 cut(s) 1499, 1659
BsmBI CGTCTC 1 cut(s) 1659
BsmFI GGGAC 2 cut(s) 713, 1189
BsnI GGCC 2 cut(s) 316, 700
Bsp1286I GDGCHC 3 cut(s) 398, 1181, 1219
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 1310, 1560
BspANI GGCC 2 cut(s) 316, 700
BspCNI CTCAG 4 cut(s) 221, 742, 787, 1411
BspLI GGNNCC 1 cut(s) 205
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 9 cut(s) 68, 198, 211, 274, 329, 1067, 1301, 1379, 1513
BspQI GCTCTTC 1 cut(s) 1224
BspTI CTTAAG 2 cut(s) 430, 1313
BsrBI CCGCTC 1 cut(s) 1562
BsrDI GCAATG 3 cut(s) 171, 275, 1236
BsrI ACTGG 5 cut(s) 329, 1029, 1125, 1145, 1344
BssECI CCNNGG 2 cut(s) 384, 591
BssNI GRCGYC 1 cut(s) 928
BssSI CACGAG 1 cut(s) 121
BssT1I CCWWGG 2 cut(s) 384, 591
Bst2BI CACGAG 1 cut(s) 121
Bst2UI CCWGG 4 cut(s) 78, 186, 498, 878
Bst4CI ACNGT 4 cut(s) 400, 1015, 1203, 1495
Bst6I CTCTTC 5 cut(s) 233, 435, 708, 1224, 1353
BstACI GRCGYC 1 cut(s) 928
BstAFI CTTAAG 2 cut(s) 430, 1313
BstAPI GCANNNNNTGC 1 cut(s) 746
BstC8I GCNNGC 3 cut(s) 55, 314, 926
BstDEI CTNAG 5 cut(s) 192, 208, 750, 774, 1419
BstDSI CCRYGG 1 cut(s) 384
BstENI CCTNNNNNAGG 2 cut(s) 148, 705
BstF5I GGATG 6 cut(s) 1052, 1111, 1159, 1178, 1609, 1711
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 2 cut(s) 164, 437
BstMAI GTCTC 2 cut(s) 1499, 1659
BstMWI GCNNNNNNNGC 9 cut(s) 746, 882, 903, 1097, 1223, 1318, 1397, 1400, 1469
BstNI CCWGG 4 cut(s) 78, 186, 498, 878
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 4 cut(s) 76, 184, 496, 876
BstSFI CTRYAG 1 cut(s) 413
BstSLI GKGCMC 1 cut(s) 398
BstX2I RGATCY 3 cut(s) 203, 1072, 1518
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 3 cut(s) 203, 1072, 1518
Bsu36I CCTNAGG 1 cut(s) 208
BsuI GTATCC 1 cut(s) 1567
BsuRI GGCC 2 cut(s) 316, 700
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 6 cut(s) 1052, 1111, 1159, 1178, 1609, 1711
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 3 cut(s) 55, 314, 926
CaiI CAGNNNCTG 1 cut(s) 1082
CfoI GCGC 2 cut(s) 164, 437
CseI GACGC 1 cut(s) 917
Csp6I GTAC 3 cut(s) 427, 1016, 1699
CviQI GTAC 3 cut(s) 427, 1016, 1699
DdeI CTNAG 5 cut(s) 192, 208, 750, 774, 1419
DrdI GACNNNNNNGTC 2 cut(s) 949, 1029
DseDI GACNNNNNNGTC 2 cut(s) 949, 1029
Eam1104I CTCTTC 5 cut(s) 233, 435, 708, 1224, 1353
EarI CTCTTC 5 cut(s) 233, 435, 708, 1224, 1353
Eco130I CCWWGG 2 cut(s) 384, 591
Eco147I AGGCCT 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 1219
Eco47III AGCGCT 1 cut(s) 436
Eco57I CTGAAG 4 cut(s) 59, 459, 828, 1416
Eco81I CCTNAGG 1 cut(s) 208
EcoNI CCTNNNNNAGG 2 cut(s) 148, 705
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 4 cut(s) 76, 184, 496, 876
EcoT14I CCWWGG 2 cut(s) 384, 591
EcoT38I GRGCYC 1 cut(s) 1219
ErhI CCWWGG 2 cut(s) 384, 591
Esp3I CGTCTC 1 cut(s) 1659
FalI AAGNNNNNCTT 4 cut(s) 301, 333, 1229, 1261
FaqI GGGAC 2 cut(s) 713, 1189
FauNDI CATATG 1 cut(s) 1118
FokI GGATG 6 cut(s) 1039, 1118, 1166, 1185, 1596, 1718
FriOI GRGCYC 1 cut(s) 1219
FspBI CTAG 8 cut(s) 111, 645, 933, 1131, 1286, 1335, 1523, 1634
GlaI GCGC 2 cut(s) 163, 436
GsaI CCCAGC 2 cut(s) 350, 1548
GsuI CTGGAG 3 cut(s) 1128, 1185, 1361
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 2 cut(s) 316, 700
HgaI GACGC 1 cut(s) 917
HhaI GCGC 2 cut(s) 164, 437
Hin1I GRCGYC 1 cut(s) 928
Hin6I GCGC 2 cut(s) 162, 435
HinP1I GCGC 2 cut(s) 162, 435
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 3 cut(s) 840, 1042, 1510
HphI GGTGA 5 cut(s) 700, 722, 1121, 1307, 1611
Hpy166II GTNNAC 2 cut(s) 836, 1050
Hpy188I TCNGA 6 cut(s) 228, 725, 777, 973, 1072, 1084
Hpy188III TCNNGA 6 cut(s) 218, 1076, 1164, 1286, 1504, 1662
Hpy8I GTNNAC 2 cut(s) 836, 1050
Hpy99I CGWCG 3 cut(s) 1673, 1676, 1679
HpyAV CCTTC 5 cut(s) 256, 712, 1163, 1530, 1709
HpyCH4III ACNGT 4 cut(s) 400, 1015, 1203, 1495
HpyCH4IV ACGT 1 cut(s) 909
HpyF10VI GCNNNNNNNGC 9 cut(s) 746, 882, 903, 1097, 1223, 1318, 1397, 1400, 1469
HpyF3I CTNAG 5 cut(s) 192, 208, 750, 774, 1419
HpySE526I ACGT 1 cut(s) 909
Hsp92I GRCGYC 1 cut(s) 928
HspAI GCGC 2 cut(s) 162, 435
LguI GCTCTTC 1 cut(s) 1224
LmnI GCTCC 4 cut(s) 593, 1176, 1567, 1627
LweI GCATC 5 cut(s) 145, 378, 736, 1144, 1308
MaeI CTAG 8 cut(s) 111, 645, 933, 1131, 1286, 1335, 1523, 1634
MaeII ACGT 1 cut(s) 909
MaeIII GTNAC 5 cut(s) 116, 125, 634, 935, 1605
MbiI CCGCTC 1 cut(s) 1562
MflI RGATCY 3 cut(s) 203, 1072, 1518
MhlI GDGCHC 3 cut(s) 398, 1181, 1219
MluCI AATT 9 cut(s) 47, 63, 86, 103, 624, 778, 791, 1006, 1467
MlyI GAGTC 5 cut(s) 124, 586, 735, 932, 1249
MseI TTAA 9 cut(s) 45, 431, 627, 809, 855, 887, 957, 1314, 1578
MslI CAYNNNNRTG 3 cut(s) 123, 919, 1409
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 2 cut(s) 430, 1313
MspR9I CCNGG 4 cut(s) 78, 186, 498, 878
MvaI CCWGG 4 cut(s) 78, 186, 498, 878
MwoI GCNNNNNNNGC 9 cut(s) 746, 882, 903, 1097, 1223, 1318, 1397, 1400, 1469
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 1118
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 4 cut(s) 116, 125, 935, 1605
NspI RCATGY 1 cut(s) 918
OliI CACNNNNGTG 1 cut(s) 123
PceI AGGCCT 1 cut(s) 700
PciSI GCTCTTC 1 cut(s) 1224
PcsI WCGNNNNNNNCGW 2 cut(s) 1674, 1680
PfeI GAWTC 6 cut(s) 351, 599, 607, 652, 1079, 1688
PflMI CCANNNNNTGG 1 cut(s) 617
PfoI TCCNGGA 1 cut(s) 76
PleI GAGTC 5 cut(s) 123, 585, 734, 932, 1248
PpsI GAGTC 5 cut(s) 123, 585, 734, 932, 1248
PshBI ATTAAT 1 cut(s) 855
Psp1406I AACGTT 1 cut(s) 909
Psp6I CCWGG 4 cut(s) 76, 184, 496, 876
PspFI CCCAGC 2 cut(s) 346, 1544
PspGI CCWGG 4 cut(s) 76, 184, 496, 876
PspN4I GGNNCC 1 cut(s) 205
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 3 cut(s) 203, 1072, 1518
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 428, 1017, 1700
RsaNI GTAC 3 cut(s) 427, 1016, 1699
RseI CAYNNNNRTG 3 cut(s) 123, 919, 1409
SapI GCTCTTC 1 cut(s) 1224
SaqAI TTAA 9 cut(s) 45, 431, 627, 809, 855, 887, 957, 1314, 1578
SchI GAGTC 5 cut(s) 124, 586, 735, 932, 1249
ScrFI CCNGG 4 cut(s) 78, 186, 498, 878
SduI GDGCHC 3 cut(s) 398, 1181, 1219
SfaNI GCATC 5 cut(s) 145, 378, 736, 1144, 1308
SfcI CTRYAG 1 cut(s) 413
SmiMI CAYNNNNRTG 3 cut(s) 123, 919, 1409
SmlI CTYRAG 4 cut(s) 218, 430, 1313, 1475
SmoI CTYRAG 4 cut(s) 218, 430, 1313, 1475
Sse9I AATT 9 cut(s) 47, 63, 86, 103, 624, 778, 791, 1006, 1467
SseBI AGGCCT 1 cut(s) 700
SsiI CCGC 2 cut(s) 1310, 1560
SspI AATATT 1 cut(s) 1658
SspMI CTAG 8 cut(s) 111, 645, 933, 1131, 1286, 1335, 1523, 1634
StuI AGGCCT 1 cut(s) 700
StyD4I CCNGG 4 cut(s) 76, 184, 496, 876
StyI CCWWGG 2 cut(s) 384, 591
TaaI ACNGT 4 cut(s) 400, 1015, 1203, 1495
TaiI ACGT 1 cut(s) 912
TaqI TCGA 6 cut(s) 26, 614, 941, 1374, 1465, 1503
TasI AATT 9 cut(s) 47, 63, 86, 103, 624, 778, 791, 1006, 1467
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 6 cut(s) 351, 599, 607, 652, 1079, 1688
Tru1I TTAA 9 cut(s) 45, 431, 627, 809, 855, 887, 957, 1314, 1578
Tru9I TTAA 9 cut(s) 45, 431, 627, 809, 855, 887, 957, 1314, 1578
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 4 cut(s) 116, 125, 935, 1605
Tsp45I GTSAC 4 cut(s) 116, 125, 935, 1605
TspDTI ATGAA 3 cut(s) 339, 500, 1701
TspGWI ACGGA 1 cut(s) 1543
TspRI CASTG 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 617
Vha464I CTTAAG 2 cut(s) 430, 1313
VspI ATTAAT 1 cut(s) 855
XagI CCTNNNNNAGG 2 cut(s) 148, 705
XapI RAATTY 3 cut(s) 47, 86, 778
XbaI TCTAGA 1 cut(s) 1285
XceI RCATGY 1 cut(s) 918
XspI CTAG 8 cut(s) 111, 645, 933, 1131, 1286, 1335, 1523, 1634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.