MD10G1268800.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006276
Physical Location & Seq
Reverse (-)
36153596 .. 36157567
3972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1268800.v1.1.491

Sequence Viewer

Length: 1722 bp
ATGATTACCACTAAAACCATGCTGTCGAGCAACAAGCTGAAGATTAAATTTGCCTGCAAAAGAATTGAAGCTGATCCCGGGAAAAAAATAGAAGCTGATTCTAATTATTCTAGGAGTCACTCACATGACTATGGGCACCTAGCCTCACTCAGGGAGGTCAGCTCAATGTCAAAATCTTCACTTCCGGGTTTTAAGAAGCGTGAACCCTCGGGGGATCTTGAGGGTCAGAAAGAGAAGAAGATGAAGATTGATCGTAGAGTGAAGGTGCAATGTTCAACGATTCTCAAAACACTGATGAATAACAAGGAAAGCTGGCCTTTCAATGAACCAGTTGATCCTGTGAAGCTGAGAATCCCTGATTACTTCCACATCATCTCTCATCCTATGGATTTGGGCACGGTAAAAGCAAAGCTGCAGAAAGATTTGTACTTCAGCGCTGAAGAGTTTGCTGGCGATGTCAGGCTGACTTTTTCTAATGCCATGCTCTATAATCCTCCTGGAAATCCTTTTCATCATATGGCAGAAAACCTGAATAGGATTTTTGAGATGAGATGGGGTCTTTTAGATGAGAAGTTGAGTCATGGAAGCTCAAAGGTTGAATCTGGGAAATCTTCGAGTGGGCAAATTAAGAAAGTTACTTGTGTGAGACAGAATCCCTGCAACACTCCTCCCTTGCAAAATAGGTTGGTGGTCAAGAGGTCTATTCCATGCGAAGAGAAGGTAAGAGTCCCTGTTGGTGCAACTGATGCTGAGGCGGAGCTTTCTAAAACAGCTCAGAATTCTAAGCCCAAATTATCATCGAAGTACTTAAATACAAGCACCGATAGTGGCAATAAACAAGCTTCAGGCATTAATGCAAAGCAGCCATCAAGCCTGGTTTGCTTAAAATGTGGTTCATGCGGAAACATTGCATGTCAATGCAGGCATTCTAGTGACTCAACCCTTACGTCCTTAAGTGACAAATCTTCTGAAAGGTCAATGGGCAGGGAGCACGGTTGGTCTAATTCTAATGTATCCAGACTGGATTGTCAAGCAAAAAGCTTGTCAACATCCCAGACGAGCAAATCAGATCCAGAATCTGATGGGGATGTAAGTGCTTTGGATGGGGAAAACATATGTTCCAGTCCTCAGCTTACAACTCCAGCATCGGATTCTGCTTCTGGAGAAGGATGGAGCACTTCTCATTTTGATGTTCAAATGTCACCCAAAAGGGCTCTCCGTGCTGCAATGCTAAAGAGCCGCTTTGCAGACACTATTTGGAAAGCTCAACAGCAGAAACTCCTAGATCATGATGACAGAAGTGATCCACTTAAGATGCAGCAGGAAAGGGCAAGATTGGAGAGGAAGCAGCGTGAAGAGAAAGCAAGGATCGAAGCTGAAATAAGAACTGCTGAAGCTGCCACACGAATGAGAGCCGAGAATGAGTTGAAGCAGCAACGGGAAAGAAAAAGAAAAGAAGCTCGAAATGCACTAGAGAAGATGAAGCGAACAGTTGAGGTTGAGACAAGCTTGAAGATCCTAGAGGAACTTGGAAGATTTACAGGGTTATCCGTATTTGCTCCTATTCTCAACTTTAATCGTGGGTCGGGGGGTTTCGGTGGATCACATCCCCCATCACCTTTGGAGCAGCTAGGTCTATTTGTAAAGCCTGAATATTCAGGAGACGACAACGATGATGAATCAATGCTGTACGAGGATGGGGAGGAAGGAGAGATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bromodomain

Protein Analysis

574

Amino Acids

63.85

Weight (kDa)

8.92

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bromodomain PF00439 91 - 175 2.9e-20 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1026
AccB1I GGYRCC 1 cut(s) 135
AciI CCGC 3 cut(s) 755, 900, 1240
AclWI GGATC 8 cut(s) 68, 222, 329, 1064, 1298, 1376, 1510, 1609
AcsI RAATTY 2 cut(s) 47, 778
AcuI CTGAAG 5 cut(s) 59, 415, 459, 828, 1413
AfaI GTAC 3 cut(s) 428, 806, 1691
AfeI AGCGCT 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 150
AflII CTTAAG 2 cut(s) 952, 1310
AgsI TTSAA 7 cut(s) 68, 276, 322, 599, 1196, 1429, 1513
AjnI CCWGG 2 cut(s) 496, 873
Alw21I GWGCWC 2 cut(s) 993, 1178
Alw26I GTCTC 3 cut(s) 640, 1496, 1656
AlwI GGATC 8 cut(s) 68, 222, 329, 1064, 1298, 1376, 1510, 1609
AlwNI CAGNNNCTG 1 cut(s) 1079
Ama87I CYCGRG 2 cut(s) 77, 208
Aor51HI AGCGCT 1 cut(s) 436
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 8 cut(s) 412, 862, 1223, 1318, 1348, 1397, 1432, 1627
ApoI RAATTY 2 cut(s) 47, 778
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
AseI ATTAAT 1 cut(s) 852
AspLEI GCGC 1 cut(s) 437
AsuC2I CCSGG 3 cut(s) 78, 79, 186
AsuHPI GGTGA 2 cut(s) 1194, 1608
AvaI CYCGRG 2 cut(s) 77, 208
BaeGI GKGCMC 2 cut(s) 138, 398
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 1 cut(s) 1216
Bbv12I GWGCWC 2 cut(s) 993, 1178
BbvCI CCTCAGC 2 cut(s) 750, 1128
BbvI GCAGC 8 cut(s) 399, 874, 1210, 1330, 1360, 1384, 1444, 1639
BccI CCATC 7 cut(s) 546, 874, 1076, 1097, 1164, 1621, 1691
BciT130I CCWGG 2 cut(s) 498, 875
BciVI GTATCC 1 cut(s) 1024
BcnI CCSGG 3 cut(s) 78, 79, 186
BcoDI GTCTC 3 cut(s) 640, 1496, 1656
BfaI CTAG 7 cut(s) 111, 140, 930, 1283, 1472, 1520, 1631
BfmI CTRYAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 438
BfrI CTTAAG 2 cut(s) 952, 1310
BfuI GTATCC 1 cut(s) 1024
BisI GCNGC 9 cut(s) 413, 863, 1224, 1240, 1319, 1349, 1398, 1433, 1628
BlsI GCNGC 9 cut(s) 414, 864, 1225, 1241, 1320, 1350, 1399, 1434, 1629
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 5 cut(s) 78, 79, 186, 498, 875
BmeT110I CYCGRG 2 cut(s) 77, 208
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 5 cut(s) 78, 79, 186, 498, 875
BmsI GCATC 3 cut(s) 736, 1154, 1305
BplI GAGNNNNNCTC 4 cut(s) 146, 178, 1165, 1197
BpmI CTGGAG 2 cut(s) 1125, 1182
Bpu10I CCTNAGC 2 cut(s) 750, 1128
BpuEI CTTGAG 1 cut(s) 239
BpuMI CCSGG 3 cut(s) 78, 79, 186
BsaJI CCNNGG 2 cut(s) 77, 207
BsaXI ACNNNNNCTCC 1 cut(s) 1701
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse1I ACTGG 3 cut(s) 329, 1026, 1122
Bse3DI GCAATG 3 cut(s) 275, 906, 1233
BseBI CCWGG 2 cut(s) 498, 875
BseDI CCNNGG 2 cut(s) 77, 207
BseGI GGATG 7 cut(s) 379, 1049, 1093, 1108, 1175, 1606, 1702
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 3 cut(s) 275, 906, 1233
BseMII CTCAG 5 cut(s) 163, 338, 741, 788, 1142
BseNI ACTGG 3 cut(s) 329, 1026, 1122
BseRI GAGGAG 1 cut(s) 657
BseSI GKGCMC 2 cut(s) 138, 398
BseXI GCAGC 8 cut(s) 399, 874, 1210, 1330, 1360, 1384, 1444, 1639
BshFI GGCC 1 cut(s) 316
BshNI GGYRCC 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 993, 1178
BsiHKCI CYCGRG 2 cut(s) 77, 208
BsiSI CCGG 2 cut(s) 78, 185
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 3 cut(s) 640, 1496, 1656
BsmBI CGTCTC 1 cut(s) 1656
BsmFI GGGAC 1 cut(s) 713
BsmI GAATGC 1 cut(s) 925
BsnI GGCC 1 cut(s) 316
BsoBI CYCGRG 2 cut(s) 77, 208
Bsp1286I GDGCHC 5 cut(s) 138, 398, 993, 1178, 1216
BspACI CCGC 3 cut(s) 755, 900, 1240
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 5 cut(s) 162, 339, 742, 787, 1141
BspHI TCATGA 1 cut(s) 1288
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 8 cut(s) 68, 222, 329, 1064, 1298, 1376, 1510, 1609
BspT107I GGYRCC 1 cut(s) 135
BspTI CTTAAG 2 cut(s) 952, 1310
BsrDI GCAATG 3 cut(s) 275, 906, 1233
BsrI ACTGG 3 cut(s) 329, 1026, 1122
BssECI CCNNGG 2 cut(s) 77, 207
Bst2UI CCWGG 2 cut(s) 498, 875
Bst4CI ACNGT 3 cut(s) 400, 995, 1492
Bst6I CTCTTC 3 cut(s) 435, 708, 1350
BstAFI CTTAAG 2 cut(s) 952, 1310
BstAPI GCANNNNNTGC 1 cut(s) 746
BstC8I GCNNGC 4 cut(s) 55, 314, 451, 923
BstDEI CTNAG 6 cut(s) 149, 347, 750, 774, 783, 1128
BstENI CCTNNNNNAGG 1 cut(s) 148
BstF5I GGATG 7 cut(s) 379, 1049, 1093, 1108, 1175, 1606, 1702
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 1 cut(s) 437
BstMAI GTCTC 3 cut(s) 640, 1496, 1656
BstMWI GCNNNNNNNGC 5 cut(s) 746, 879, 1220, 1397, 1466
BstNI CCWGG 2 cut(s) 498, 875
BstNSI RCATGY 1 cut(s) 915
BstSCI CCNGG 5 cut(s) 76, 77, 184, 496, 873
BstSFI CTRYAG 1 cut(s) 413
BstSLI GKGCMC 2 cut(s) 138, 398
BstV1I GCAGC 8 cut(s) 399, 874, 1210, 1330, 1360, 1384, 1444, 1639
BstX2I RGATCY 3 cut(s) 214, 1069, 1515
BstYI RGATCY 3 cut(s) 214, 1069, 1515
BsuI GTATCC 1 cut(s) 1024
BsuRI GGCC 1 cut(s) 316
BtgZI GCGATG 1 cut(s) 468
BtsCI GGATG 7 cut(s) 379, 1049, 1093, 1108, 1175, 1606, 1702
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 4 cut(s) 55, 314, 451, 923
CaiI CAGNNNCTG 1 cut(s) 1079
CciI TCATGA 1 cut(s) 1288
CfoI GCGC 1 cut(s) 437
Cfr9I CCCGGG 1 cut(s) 77
Csp6I GTAC 3 cut(s) 427, 805, 1690
CviAII CATG 8 cut(s) 19, 125, 481, 581, 708, 897, 912, 1289
CviQI GTAC 3 cut(s) 427, 805, 1690
DdeI CTNAG 6 cut(s) 149, 347, 750, 774, 783, 1128
DrdI GACNNNNNNGTC 1 cut(s) 1026
DseDI GACNNNNNNGTC 1 cut(s) 1026
Eam1104I CTCTTC 3 cut(s) 435, 708, 1350
EarI CTCTTC 3 cut(s) 435, 708, 1350
EciI GGCGGA 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 1216
Eco47III AGCGCT 1 cut(s) 436
Eco57I CTGAAG 5 cut(s) 59, 415, 459, 828, 1413
Eco88I CYCGRG 2 cut(s) 77, 208
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 496, 873
EcoT38I GRGCYC 1 cut(s) 1216
Esp3I CGTCTC 1 cut(s) 1656
FaeI CATG 8 cut(s) 22, 128, 484, 584, 711, 900, 915, 1292
FalI AAGNNNNNCTT 4 cut(s) 301, 333, 1226, 1258
FaqI GGGAC 1 cut(s) 713
FatI CATG 8 cut(s) 18, 124, 480, 580, 707, 896, 911, 1288
FauNDI CATATG 2 cut(s) 516, 1115
Fnu4HI GCNGC 9 cut(s) 413, 863, 1224, 1240, 1319, 1349, 1398, 1433, 1628
FokI GGATG 7 cut(s) 366, 1036, 1100, 1115, 1182, 1593, 1709
FriOI GRGCYC 1 cut(s) 1216
Fsp4HI GCNGC 9 cut(s) 413, 863, 1224, 1240, 1319, 1349, 1398, 1433, 1628
FspBI CTAG 7 cut(s) 111, 140, 930, 1283, 1472, 1520, 1631
GlaI GCGC 1 cut(s) 436
GluI GCNGC 9 cut(s) 413, 863, 1224, 1240, 1319, 1349, 1398, 1433, 1628
GsuI CTGGAG 2 cut(s) 1125, 1182
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 1 cut(s) 316
HapII CCGG 2 cut(s) 78, 185
HhaI GCGC 1 cut(s) 437
Hin1II CATG 8 cut(s) 22, 128, 484, 584, 711, 900, 915, 1292
Hin6I GCGC 1 cut(s) 435
HinP1I GCGC 1 cut(s) 435
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HindIII AAGCTT 3 cut(s) 840, 1039, 1507
HpaII CCGG 2 cut(s) 78, 185
HphI GGTGA 2 cut(s) 1194, 1608
Hpy166II GTNNAC 2 cut(s) 203, 1047
Hpy188I TCNGA 6 cut(s) 228, 777, 970, 1069, 1081, 1150
Hpy188III TCNNGA 7 cut(s) 218, 694, 1017, 1073, 1161, 1289, 1659
Hpy8I GTNNAC 2 cut(s) 203, 1047
HpyAV CCTTC 4 cut(s) 256, 712, 1160, 1700
HpyCH4III ACNGT 3 cut(s) 400, 995, 1492
HpyCH4IV ACGT 1 cut(s) 947
HpyF10VI GCNNNNNNNGC 5 cut(s) 746, 879, 1220, 1397, 1466
HpyF3I CTNAG 6 cut(s) 149, 347, 750, 774, 783, 1128
HpySE526I ACGT 1 cut(s) 947
Hsp92II CATG 8 cut(s) 22, 128, 484, 584, 711, 900, 915, 1292
HspAI GCGC 1 cut(s) 435
LmnI GCTCC 5 cut(s) 757, 988, 1173, 1564, 1624
Lsp1109I GCAGC 8 cut(s) 399, 874, 1210, 1330, 1360, 1384, 1444, 1639
LweI GCATC 3 cut(s) 736, 1154, 1305
MaeI CTAG 7 cut(s) 111, 140, 930, 1283, 1472, 1520, 1631
MaeII ACGT 1 cut(s) 947
MaeIII GTNAC 5 cut(s) 116, 634, 932, 956, 1200
MflI RGATCY 3 cut(s) 214, 1069, 1515
MhlI GDGCHC 5 cut(s) 138, 398, 993, 1178, 1216
MluCI AATT 7 cut(s) 47, 63, 103, 624, 778, 791, 1003
MlyI GAGTC 4 cut(s) 124, 586, 735, 929
MseI TTAA 9 cut(s) 45, 192, 627, 809, 852, 884, 953, 1311, 1575
MslI CAYNNNNRTG 6 cut(s) 123, 129, 916, 930, 1188, 1406
MspCI CTTAAG 2 cut(s) 952, 1310
MspI CCGG 2 cut(s) 78, 185
MspR9I CCNGG 5 cut(s) 78, 79, 186, 498, 875
Mva1269I GAATGC 1 cut(s) 925
MvaI CCWGG 2 cut(s) 498, 875
MwoI GCNNNNNNNGC 5 cut(s) 746, 879, 1220, 1397, 1466
NciI CCSGG 3 cut(s) 78, 79, 186
NdeI CATATG 2 cut(s) 516, 1115
NlaIII CATG 8 cut(s) 22, 128, 484, 584, 711, 900, 915, 1292
NlaIV GGNNCC 1 cut(s) 137
NmeAIII GCCGAG 1 cut(s) 1441
NmuCI GTSAC 4 cut(s) 116, 932, 956, 1200
NspI RCATGY 1 cut(s) 915
PagI TCATGA 1 cut(s) 1288
PctI GAATGC 1 cut(s) 925
PfeI GAWTC 8 cut(s) 98, 280, 351, 599, 652, 1076, 1151, 1679
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 9 cut(s) 414, 864, 1225, 1241, 1320, 1350, 1399, 1434, 1629
PleI GAGTC 4 cut(s) 123, 585, 734, 929
PpsI GAGTC 4 cut(s) 123, 585, 734, 929
PshBI ATTAAT 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 496, 873
PspGI CCWGG 2 cut(s) 496, 873
PspN4I GGNNCC 1 cut(s) 137
PstI CTGCAG 1 cut(s) 417
PstNI CAGNNNCTG 1 cut(s) 1079
PsuI RGATCY 3 cut(s) 214, 1069, 1515
RsaI GTAC 3 cut(s) 428, 806, 1691
RsaNI GTAC 3 cut(s) 427, 805, 1690
RseI CAYNNNNRTG 6 cut(s) 123, 129, 916, 930, 1188, 1406
SaqAI TTAA 9 cut(s) 45, 192, 627, 809, 852, 884, 953, 1311, 1575
SatI GCNGC 9 cut(s) 413, 863, 1224, 1240, 1319, 1349, 1398, 1433, 1628
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 4 cut(s) 124, 586, 735, 929
ScrFI CCNGG 5 cut(s) 78, 79, 186, 498, 875
SduI GDGCHC 5 cut(s) 138, 398, 993, 1178, 1216
SfaNI GCATC 3 cut(s) 736, 1154, 1305
SfcI CTRYAG 1 cut(s) 413
SmaI CCCGGG 1 cut(s) 79
SmiMI CAYNNNNRTG 6 cut(s) 123, 129, 916, 930, 1188, 1406
SmlI CTYRAG 3 cut(s) 218, 952, 1310
SmoI CTYRAG 3 cut(s) 218, 952, 1310
Sse9I AATT 7 cut(s) 47, 63, 103, 624, 778, 791, 1003
SsiI CCGC 3 cut(s) 755, 900, 1240
SspI AATATT 1 cut(s) 1655
SspMI CTAG 7 cut(s) 111, 140, 930, 1283, 1472, 1520, 1631
StyD4I CCNGG 5 cut(s) 76, 77, 184, 496, 873
TaaI ACNGT 3 cut(s) 400, 995, 1492
TaiI ACGT 1 cut(s) 950
TaqI TCGA 5 cut(s) 26, 614, 800, 1371, 1462
TasI AATT 7 cut(s) 47, 63, 103, 624, 778, 791, 1003
TatI WGTACW 2 cut(s) 426, 804
TauI GCSGC 1 cut(s) 1242
TfiI GAWTC 8 cut(s) 98, 280, 351, 599, 652, 1076, 1151, 1679
Tru1I TTAA 9 cut(s) 45, 192, 627, 809, 852, 884, 953, 1311, 1575
Tru9I TTAA 9 cut(s) 45, 192, 627, 809, 852, 884, 953, 1311, 1575
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 4 cut(s) 116, 932, 956, 1200
TseI GCWGC 8 cut(s) 412, 862, 1223, 1318, 1348, 1397, 1432, 1627
Tsp45I GTSAC 4 cut(s) 116, 932, 956, 1200
TspDTI ATGAA 7 cut(s) 257, 311, 339, 500, 885, 1496, 1692
TspGWI ACGGA 2 cut(s) 1208, 1540
TspMI CCCGGG 1 cut(s) 77
TspRI CASTG 1 cut(s) 297
Vha464I CTTAAG 2 cut(s) 952, 1310
VspI ATTAAT 1 cut(s) 852
XagI CCTNNNNNAGG 1 cut(s) 148
XapI RAATTY 2 cut(s) 47, 778
XceI RCATGY 1 cut(s) 915
XmaI CCCGGG 1 cut(s) 77
XspI CTAG 7 cut(s) 111, 140, 930, 1283, 1472, 1520, 1631
ZrmI AGTACT 1 cut(s) 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.