MD05G1299300.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005527
Physical Location & Seq
Forward (+)
43289198 .. 43292861
3664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1299300.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGATGAGCTAGAATTTGGTAATCAGGAAGCTGAGAAGAGTAACGAGATCAGCGAGGAACAAGTAGAAAACGGATCAGTGGGATCATCCCAGAAATGCTCATCCTTCGATTTGAATGAAGAAGCAAGTAGTGACAATGAAGAAAGAGGCCAAGGGAATAATGCTGCATATAATGATAGTGAAGGAAATGAAAGGCGGAGGGTTAAAGTTAGGCAATATGTGAGATCAAAAATGCCTCGTCTACGGTGGACTCCTCAACTCCATCTTTCTTTTGTGCATGCTGTTGAACGACTGGGTGGCCAAGATAGAGCAACTCCCAAGTTAATTCTTCAGCTAATGAATGTGAGAGGACTTAGCATTTCTCATGTAAAGAGTCACTTGCAGATGTATAGAAGTAAGAAGCTTGACGGGAATGGCCAAGTATTATCTGAAAAACACGAATCCAAGCGTGGAAGGGATAACATATCTTGCAAGGTGCACCAAACAATCAACCAGCCTTGTCGACATGTATATTTTGGAATGGACAACGGTAGCATTGTTGTAACAACAAGTCCACACGACTGTCAGAGCCAACCACAGCCAGATTTTAAAGCTGTCTCAAGGTCAAGTTCTTTACTAAACGAAGAACTTGTACAAGGCAACAACTCCAACAAATCTATGGATACAATCTTGCGAACTGTACCAATAAACCCTAGCCGGCTTCTGGAGGACAAGAGGTGGCCTCCATCTGAAACGATTAACCAACAACAATGGAGGGCTAAAAGAATACCTGCTGCCAAAATCAGTTGGTCAGATGATTGTAATGGCTCCCAATCTGACCATCTAATGCATCATATGCACACCACACCGAGGTTGATAAGGGAATCCCACAATATAAGGCCACCAAACTGGAACCTTGGAGAGAGCAGAAACATAAGGCAGTTTCCATCAAACTCCCACAATTCTATCACCAATTCCAGTTGCTACAAAACTGAATTCGAGCCGCCATTTGGGTTTGAGTTAAATGAAGAAAAAAGCTCGAAGTACAAAGGGTGGATGCCACAACTGGAGTTGTGTCTGAGTCAAAGTGTTGAGAATTATGAGGAAACGAAGATTATTTCCAGACATCGTATGGTTGGTGAAGAACTTTATGGAACTGGGCAAAGAGTTAAACAGGAAATTAGCACTAAACTTTCTCTTTCTCTTTAA

Protein Analysis

396

Amino Acids

45.21

Weight (kDa)

8.11

Isoelectric Point (pI)

65.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 80 - 131 1.5e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 778
AccI GTMKAC 2 cut(s) 241, 502
AciI CCGC 2 cut(s) 196, 983
AclWI GGATC 2 cut(s) 82, 91
AcoI YGGCCR 2 cut(s) 298, 415
AcsI RAATTY 2 cut(s) 14, 974
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 3 cut(s) 633, 681, 1025
AfiI CCNNNNNNNGG 3 cut(s) 703, 849, 989
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 2 cut(s) 115, 287
AluBI AGCT 6 cut(s) 10, 32, 334, 403, 593, 1017
AluI AGCT 6 cut(s) 10, 32, 334, 403, 593, 1017
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 601
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 2 cut(s) 82, 91
AoxI GGCC 5 cut(s) 148, 298, 415, 719, 878
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 164, 773
ApoI RAATTY 2 cut(s) 14, 974
AsuHPI GGTGA 2 cut(s) 940, 1130
BaeGI GKGCMC 1 cut(s) 480
BalI TGGCCA 2 cut(s) 300, 417
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 151, 760
BccI CCATC 4 cut(s) 270, 733, 828, 934
BciVI GTATCC 1 cut(s) 655
BcoDI GTCTC 1 cut(s) 601
BfaI CTAG 2 cut(s) 11, 693
BfuAI ACCTGC 1 cut(s) 778
BfuI GTATCC 1 cut(s) 655
BisI GCNGC 3 cut(s) 165, 774, 983
BlsI GCNGC 3 cut(s) 166, 775, 984
BmiI GGNNCC 2 cut(s) 808, 893
BmrI ACTGGG 2 cut(s) 302, 1146
BmsI GCATC 2 cut(s) 838, 1026
BmuI ACTGGG 2 cut(s) 302, 1146
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 2 cut(s) 725, 1067
BpuEI CTTGAG 1 cut(s) 583
BsaJI CCNNGG 3 cut(s) 151, 848, 895
Bsc4I CCNNNNNNNGG 3 cut(s) 703, 849, 989
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 5 cut(s) 297, 893, 957, 1050, 1141
BseDI CCNNGG 3 cut(s) 151, 848, 895
BseGI GGATG 4 cut(s) 10, 86, 101, 1041
BseLI CCNNNNNNNGG 3 cut(s) 703, 849, 989
BseMII CTCAG 2 cut(s) 24, 1049
BseNI ACTGG 5 cut(s) 297, 893, 957, 1050, 1141
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 2 cut(s) 151, 760
BshFI GGCC 5 cut(s) 150, 300, 417, 721, 880
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 1 cut(s) 697
BslI CCNNNNNNNGG 3 cut(s) 703, 849, 989
BsmAI GTCTC 1 cut(s) 601
BsnI GGCC 5 cut(s) 150, 300, 417, 721, 880
Bsp1286I GDGCHC 1 cut(s) 480
Bsp1407I TGTACA 1 cut(s) 631
Bsp143I GATC 4 cut(s) 48, 74, 83, 224
BspACI CCGC 2 cut(s) 196, 983
BspANI GGCC 5 cut(s) 150, 300, 417, 721, 880
BspCNI CTCAG 2 cut(s) 25, 1050
BspLI GGNNCC 2 cut(s) 808, 893
BspMI ACCTGC 1 cut(s) 778
BspPI GGATC 2 cut(s) 82, 91
BsrFI RCCGGY 1 cut(s) 696
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 5 cut(s) 297, 893, 957, 1050, 1141
BssAI RCCGGY 1 cut(s) 696
BssECI CCNNGG 3 cut(s) 151, 848, 895
BssMI GATC 4 cut(s) 48, 74, 83, 224
BssT1I CCWWGG 2 cut(s) 151, 895
Bst4CI ACNGT 4 cut(s) 246, 530, 563, 679
Bst6I CTCTTC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 835
BstAUI TGTACA 1 cut(s) 631
BstC8I GCNNGC 2 cut(s) 279, 698
BstDEI CTNAG 3 cut(s) 33, 353, 1058
BstF5I GGATG 4 cut(s) 10, 86, 101, 1041
BstKTI GATC 4 cut(s) 51, 77, 86, 227
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 4 cut(s) 48, 74, 83, 224
BstMWI GCNNNNNNNGC 1 cut(s) 835
BstNSI RCATGY 2 cut(s) 281, 509
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 151, 760
BstXI CCANNNNNNTGG 1 cut(s) 888
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 5 cut(s) 150, 300, 417, 721, 880
BtsCI GGATG 4 cut(s) 10, 86, 101, 1041
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 778
Cac8I GCNNGC 2 cut(s) 279, 698
Cfr10I RCCGGY 1 cut(s) 696
Csp6I GTAC 3 cut(s) 632, 680, 1024
CviAII CATG 3 cut(s) 278, 365, 506
CviQI GTAC 3 cut(s) 632, 680, 1024
DdeI CTNAG 3 cut(s) 33, 353, 1058
DpnI GATC 4 cut(s) 50, 76, 85, 226
DpnII GATC 4 cut(s) 48, 74, 83, 224
DraI TTTAAA 1 cut(s) 589
EaeI YGGCCR 2 cut(s) 298, 415
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 151, 895
Eco57I CTGAAG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 974
EcoT14I CCWWGG 2 cut(s) 151, 895
EcoT22I ATGCAT 1 cut(s) 831
ErhI CCWWGG 2 cut(s) 151, 895
FaeI CATG 3 cut(s) 281, 368, 509
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FatI CATG 3 cut(s) 277, 364, 505
FauNDI CATATG 1 cut(s) 834
FblI GTMKAC 2 cut(s) 241, 502
Fnu4HI GCNGC 3 cut(s) 165, 774, 983
FokI GGATG 4 cut(s) 17, 73, 88, 1048
Fsp4HI GCNGC 3 cut(s) 165, 774, 983
FspBI CTAG 2 cut(s) 11, 693
GluI GCNGC 3 cut(s) 165, 774, 983
GsuI CTGGAG 2 cut(s) 725, 1067
HaeIII GGCC 5 cut(s) 150, 300, 417, 721, 880
HapII CCGG 1 cut(s) 697
Hin1II CATG 3 cut(s) 281, 368, 509
HincII GTYRAC 1 cut(s) 503
HindII GTYRAC 1 cut(s) 503
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 5 cut(s) 250, 373, 440, 863, 1060
HpaII CCGG 1 cut(s) 697
HphI GGTGA 2 cut(s) 940, 1130
Hpy166II GTNNAC 5 cut(s) 242, 249, 478, 503, 554
Hpy188I TCNGA 6 cut(s) 430, 567, 730, 793, 817, 1059
Hpy188III TCNNGA 3 cut(s) 26, 704, 1101
Hpy8I GTNNAC 5 cut(s) 242, 249, 478, 503, 554
HpyAV CCTTC 3 cut(s) 115, 176, 447
HpyCH4III ACNGT 4 cut(s) 246, 530, 563, 679
HpyCH4V TGCA 7 cut(s) 167, 277, 382, 471, 478, 829, 838
HpyF10VI GCNNNNNNNGC 1 cut(s) 835
HpyF3I CTNAG 3 cut(s) 33, 353, 1058
Hsp92II CATG 3 cut(s) 281, 368, 509
KroI GCCGGC 1 cut(s) 696
KroNI GCCGGC 1 cut(s) 698
Kzo9I GATC 4 cut(s) 48, 74, 83, 224
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 2 cut(s) 151, 760
LweI GCATC 2 cut(s) 838, 1026
MaeI CTAG 2 cut(s) 11, 693
MaeIII GTNAC 4 cut(s) 41, 131, 374, 541
MalI GATC 4 cut(s) 50, 76, 85, 226
MboI GATC 4 cut(s) 48, 74, 83, 224
MboII GAAGA 8 cut(s) 49, 131, 152, 320, 635, 1019, 1102, 1133
MhlI GDGCHC 1 cut(s) 480
MlsI TGGCCA 2 cut(s) 300, 417
MluCI AATT 7 cut(s) 14, 324, 940, 952, 974, 1075, 1158
MluNI TGGCCA 2 cut(s) 300, 417
MlyI GAGTC 3 cut(s) 244, 382, 1069
MmeI TCCRAC 1 cut(s) 672
Mox20I TGGCCA 2 cut(s) 300, 417
Mph1103I ATGCAT 1 cut(s) 831
MroNI GCCGGC 1 cut(s) 696
MscI TGGCCA 2 cut(s) 300, 417
MseI TTAA 7 cut(s) 204, 323, 588, 738, 1001, 1149, 1186
Msp20I TGGCCA 2 cut(s) 300, 417
MspI CCGG 1 cut(s) 697
MwoI GCNNNNNNNGC 1 cut(s) 835
NaeI GCCGGC 1 cut(s) 698
NdeI CATATG 1 cut(s) 834
NdeII GATC 4 cut(s) 48, 74, 83, 224
NgoMIV GCCGGC 1 cut(s) 696
NlaIII CATG 3 cut(s) 281, 368, 509
NlaIV GGNNCC 2 cut(s) 808, 893
NmuCI GTSAC 2 cut(s) 131, 374
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 2 cut(s) 281, 509
PaeI GCATGC 1 cut(s) 281
PciI ACATGT 1 cut(s) 505
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PdiI GCCGGC 1 cut(s) 698
PfeI GAWTC 2 cut(s) 440, 863
PkrI GCNGC 3 cut(s) 166, 775, 984
PleI GAGTC 3 cut(s) 244, 381, 1068
PpsI GAGTC 3 cut(s) 244, 381, 1068
PscI ACATGT 1 cut(s) 505
PspN4I GGNNCC 2 cut(s) 808, 893
RsaI GTAC 3 cut(s) 633, 681, 1025
RsaNI GTAC 3 cut(s) 632, 680, 1024
SalI GTCGAC 1 cut(s) 501
SaqAI TTAA 7 cut(s) 204, 323, 588, 738, 1001, 1149, 1186
SatI GCNGC 3 cut(s) 165, 774, 983
Sau3AI GATC 4 cut(s) 48, 74, 83, 224
SchI GAGTC 3 cut(s) 244, 382, 1069
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 2 cut(s) 838, 1026
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
SphI GCATGC 1 cut(s) 281
Sse9I AATT 7 cut(s) 14, 324, 940, 952, 974, 1075, 1158
SsiI CCGC 2 cut(s) 196, 983
SspMI CTAG 2 cut(s) 11, 693
StyI CCWWGG 2 cut(s) 151, 895
TaaI ACNGT 4 cut(s) 246, 530, 563, 679
TaqI TCGA 4 cut(s) 108, 502, 978, 1019
TasI AATT 7 cut(s) 14, 324, 940, 952, 974, 1075, 1158
TatI WGTACW 2 cut(s) 631, 1023
TauI GCSGC 1 cut(s) 985
TfiI GAWTC 2 cut(s) 440, 863
Tru1I TTAA 7 cut(s) 204, 323, 588, 738, 1001, 1149, 1186
Tru9I TTAA 7 cut(s) 204, 323, 588, 738, 1001, 1149, 1186
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 2 cut(s) 131, 374
TseI GCWGC 2 cut(s) 164, 773
Tsp45I GTSAC 2 cut(s) 131, 374
TspDTI ATGAA 5 cut(s) 132, 153, 204, 353, 1020
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 84
VneI GTGCAC 1 cut(s) 476
XapI RAATTY 2 cut(s) 14, 974
XceI RCATGY 2 cut(s) 281, 509
XcmI CCANNNNNNNNNTGG 2 cut(s) 655, 1108
XmiI GTMKAC 2 cut(s) 241, 502
XspI CTAG 2 cut(s) 11, 693
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.