Rh5DG081200
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005527
Physical Location & Seq
Reverse (-)
7114365 .. 7118399
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG081200.1

Sequence Viewer

Length: 1161 bp
ATGATGAATGAGGTGGAGTTAATTGGTGATCAAAAAGAAAAGACTGACAAAAATATCAGTGAGGACCAAGCAGAAAACATATCGGTGGGTTCTTCCCAGAAATGCTCATGCTTTGATTTAAATGAAGAAGCCAGCGATAACAGCGACAATGATAACAGCGATAACAGCAATGCCAACGTTAACCTGGATGATCATGAACTTAGTACAGTTAGAAATGATGAAAGAAGAGCCCTAGCCCAGGGAAACATTAATAGTAATGCAGCTACTATTAGTAATAGCTCTAGGGTTAAAGTAAGGCCATATGTACGATCCAAACTTCCTCGTCTGCGGTGGACACCCCAACTCCATCTTTCATTTTTGCATGCTTTGGAATTACTGGGAGGGCATGAGAGAGCAACTCCCAAGTTGGTTCTTCAGCTAATGAATGTGAGAGGACTAAGCATTTCCCACGTAAAGAGTCACTTGCAGATGTATCGAAGTAAGAAGCTTGATAGGGATGGTCAAGTAGTATCTGATGAGAAAACTTACGATTACACGGATGGAAGAGAATCAAGGGATCACATACCTAACTTCGATTCGAGGTCAAGTTCTCTGACAAAGAAAGAAGTCGTACAAGGCAACAATTTGAACAAAGCTTTGCATCAAACAAGCACTCCCATAAACCCTAGCCGATTACGTGAGGAAAAGAGGTGGCCTCCATTTCAAAGCATTAGCAAAAGCTGGAGGGCCAGAAGATTTGCTTCCAACAATATCAATTGGTCAAATAGTCATAACGGCTCATCCCAATCTGATATAATGCATCACATGATTAGCAACAGACCAACAGATCATGATCTACGTTCTTGGAACCTTGAAGGTCATAGCAATTCAAGTAGCTACAAACAAGAATTCGAACCGCCATTTAGGCTTGAGTGGAACAAGGAAAAAATTTTGAAGTACAAGGAATGGATGCCTGATTTGGAGTTGGGTCTGCAAAGCCAAAGAGTTTGGAATGAGATCAATGACGAGACTCAGCATCATCAGACGATTAGTCACGCAGAAGGAAATTATGAAACTGTGCAAGGAGTTATAGAGACTTCACAATATTACAAAGATCATCAATTCGAGGACATGAATAGCAAACAAGAAACAATTAGCACCAAGCTTTCCCTTTCTCTTTAA

Protein Analysis

386

Amino Acids

44.56

Weight (kDa)

6.29

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 108 - 159 3.7e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 328, 896
AclI AACGTT 1 cut(s) 177
AclWI GGATC 2 cut(s) 303, 564
AcsI RAATTY 2 cut(s) 887, 927
AcuI CTGAAG 1 cut(s) 398
AfaI GTAC 4 cut(s) 205, 306, 612, 938
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 5 cut(s) 628, 704, 854, 870, 934
AhdI GACNNNNNGTC 1 cut(s) 1029
AjnI CCWGG 2 cut(s) 183, 237
AluBI AGCT 8 cut(s) 263, 279, 418, 487, 635, 720, 876, 1144
AluI AGCT 8 cut(s) 263, 279, 418, 487, 635, 720, 876, 1144
Alw26I GTCTC 2 cut(s) 1001, 1067
AlwI GGATC 2 cut(s) 303, 564
AoxI GGCC 3 cut(s) 296, 692, 726
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 2 cut(s) 887, 927
AseI ATTAAT 1 cut(s) 249
AspS9I GGNCC 2 cut(s) 64, 726
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 891
AvaII GGWCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 232
BbvI GCAGC 1 cut(s) 272
BccI CCATC 3 cut(s) 354, 491, 533
BceAI ACGGC 1 cut(s) 790
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 2 cut(s) 185, 239
BclI TGATCA 2 cut(s) 28, 190
BcoDI GTCTC 2 cut(s) 1001, 1067
BfaI CTAG 3 cut(s) 233, 282, 666
BglI GCCNNNNNGGC 1 cut(s) 904
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 185, 239
Bme18I GGWCC 1 cut(s) 64
BmeRI GACNNNNNGTC 1 cut(s) 1029
BmgT120I GGNCC 2 cut(s) 64, 726
BmiI GGNNCC 1 cut(s) 848
BmrFI CCNGG 2 cut(s) 185, 239
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 4 cut(s) 649, 808, 939, 1024
BmuI ACTGGG 1 cut(s) 386
BplI GAGNNNNNCTC 4 cut(s) 382, 414, 679, 711
BpmI CTGGAG 1 cut(s) 742
Bpu14I TTCGAA 1 cut(s) 891
BpuEI CTTGAG 1 cut(s) 929
BsaAI YACGTR 2 cut(s) 451, 677
BsaBI GATNNNNATC 1 cut(s) 831
BsaJI CCNNGG 2 cut(s) 237, 238
BsaXI ACNNNNNCTCC 4 cut(s) 327, 357, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 831
BseBI CCWGG 2 cut(s) 185, 239
BseDI CCNNGG 2 cut(s) 237, 238
BseGI GGATG 5 cut(s) 193, 502, 544, 779, 954
BseJI GATNNNNATC 1 cut(s) 831
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 1025
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 272
BshFI GGCC 3 cut(s) 298, 694, 728
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 1001, 1067
BsnI GGCC 3 cut(s) 298, 694, 728
Bsp119I TTCGAA 1 cut(s) 891
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
BspACI CCGC 2 cut(s) 328, 896
BspANI GGCC 3 cut(s) 298, 694, 728
BspCNI CTCAG 1 cut(s) 1024
BspHI TCATGA 2 cut(s) 193, 829
BspLI GGNNCC 1 cut(s) 848
BspPI GGATC 2 cut(s) 303, 564
BspQI GCTCTTC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 891
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 237, 238
BssMI GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
Bst2UI CCWGG 2 cut(s) 185, 239
Bst4CI ACNGT 2 cut(s) 208, 1057
Bst6I CTCTTC 2 cut(s) 220, 538
BstBAI YACGTR 2 cut(s) 451, 677
BstBI TTCGAA 1 cut(s) 891
BstC8I GCNNGC 2 cut(s) 133, 363
BstDEI CTNAG 3 cut(s) 200, 437, 1011
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 5 cut(s) 193, 502, 544, 779, 954
BstKTI GATC 8 cut(s) 31, 193, 311, 559, 829, 835, 999, 1096
BstMAI GTCTC 2 cut(s) 1001, 1067
BstMBI GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
BstMWI GCNNNNNNNGC 3 cut(s) 141, 165, 904
BstNI CCWGG 2 cut(s) 185, 239
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 183, 237
BstV1I GCAGC 1 cut(s) 272
BsuRI GGCC 3 cut(s) 298, 694, 728
BtsCI GGATG 5 cut(s) 193, 502, 544, 779, 954
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 2 cut(s) 133, 363
CciI TCATGA 2 cut(s) 193, 829
Cfr13I GGNCC 2 cut(s) 64, 726
Csp6I GTAC 4 cut(s) 204, 305, 611, 937
CviAII CATG 7 cut(s) 108, 194, 362, 386, 805, 830, 1111
CviQI GTAC 4 cut(s) 204, 305, 611, 937
DdeI CTNAG 3 cut(s) 200, 437, 1011
DpnI GATC 8 cut(s) 30, 192, 310, 558, 828, 834, 998, 1095
DpnII GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
DraI TTTAAA 1 cut(s) 120
DriI GACNNNNNGTC 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 220, 538
Eam1105I GACNNNNNGTC 1 cut(s) 1029
EarI CTCTTC 2 cut(s) 220, 538
Eco24I GRGCYC 1 cut(s) 232
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 398
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 887
EcoRII CCWGG 2 cut(s) 183, 237
EcoT22I ATGCAT 1 cut(s) 801
EcoT38I GRGCYC 1 cut(s) 232
FaeI CATG 7 cut(s) 111, 197, 365, 389, 808, 833, 1114
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 724, 756
FatI CATG 7 cut(s) 107, 193, 361, 385, 804, 829, 1110
FauNDI CATATG 1 cut(s) 301
FbaI TGATCA 2 cut(s) 28, 190
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 5 cut(s) 200, 509, 551, 766, 961
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 3 cut(s) 233, 282, 666
GluI GCNGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 742
HaeIII GGCC 3 cut(s) 298, 694, 728
Hin1II CATG 7 cut(s) 111, 197, 365, 389, 808, 833, 1114
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 3 cut(s) 485, 633, 1142
HinfI GANTC 4 cut(s) 457, 548, 575, 1009
HpaI GTTAAC 1 cut(s) 181
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 181, 333
Hpy188I TCNGA 4 cut(s) 514, 594, 790, 1023
Hpy188III TCNNGA 2 cut(s) 194, 830
Hpy8I GTNNAC 2 cut(s) 181, 333
HpyAV CCTTC 2 cut(s) 848, 1034
HpyCH4III ACNGT 2 cut(s) 208, 1057
HpyCH4IV ACGT 4 cut(s) 177, 450, 676, 838
HpyCH4V TGCA 7 cut(s) 260, 361, 466, 640, 799, 973, 1060
HpyF10VI GCNNNNNNNGC 3 cut(s) 141, 165, 904
HpyF3I CTNAG 3 cut(s) 200, 437, 1011
HpySE526I ACGT 4 cut(s) 177, 450, 676, 838
Hsp92II CATG 7 cut(s) 111, 197, 365, 389, 808, 833, 1114
Ksp22I TGATCA 2 cut(s) 28, 190
KspAI GTTAAC 1 cut(s) 181
Kzo9I GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
LguI GCTCTTC 1 cut(s) 220
Lsp1109I GCAGC 1 cut(s) 272
LweI GCATC 4 cut(s) 649, 808, 939, 1024
MaeI CTAG 3 cut(s) 233, 282, 666
MaeII ACGT 4 cut(s) 177, 450, 676, 838
MaeIII GTNAC 2 cut(s) 458, 1031
MalI GATC 8 cut(s) 30, 192, 310, 558, 828, 834, 998, 1095
MboI GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
MboII GAAGA 6 cut(s) 84, 137, 237, 404, 555, 744
MfeI CAATTG 1 cut(s) 754
MhlI GDGCHC 1 cut(s) 232
MlyI GAGTC 2 cut(s) 466, 1003
MmeI TCCRAC 1 cut(s) 768
Mph1103I ATGCAT 1 cut(s) 801
MseI TTAA 6 cut(s) 20, 119, 180, 249, 288, 1159
MslI CAYNNNNRTG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 185, 239
MunI CAATTG 1 cut(s) 754
MvaI CCWGG 2 cut(s) 185, 239
MwoI GCNNNNNNNGC 3 cut(s) 141, 165, 904
NdeI CATATG 1 cut(s) 301
NdeII GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
NlaIII CATG 7 cut(s) 111, 197, 365, 389, 808, 833, 1114
NlaIV GGNNCC 1 cut(s) 848
NmuCI GTSAC 2 cut(s) 458, 1031
NsiI ATGCAT 1 cut(s) 801
NspI RCATGY 1 cut(s) 365
NspV TTCGAA 1 cut(s) 891
PaeI GCATGC 1 cut(s) 365
PagI TCATGA 2 cut(s) 193, 829
PasI CCCWGGG 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 220
PfeI GAWTC 2 cut(s) 548, 575
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 2 cut(s) 465, 1003
PpsI GAGTC 2 cut(s) 465, 1003
Ppu21I YACGTR 2 cut(s) 451, 677
PshBI ATTAAT 1 cut(s) 249
Psp1406I AACGTT 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 183, 237
PspGI CCWGG 2 cut(s) 183, 237
PspN4I GGNNCC 1 cut(s) 848
PspPI GGNCC 2 cut(s) 64, 726
RsaI GTAC 4 cut(s) 205, 306, 612, 938
RsaNI GTAC 4 cut(s) 204, 305, 611, 937
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 6 cut(s) 20, 119, 180, 249, 288, 1159
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 8 cut(s) 28, 190, 308, 556, 826, 832, 996, 1093
Sau96I GGNCC 2 cut(s) 64, 726
SchI GAGTC 2 cut(s) 466, 1003
ScrFI CCNGG 2 cut(s) 185, 239
SduI GDGCHC 1 cut(s) 232
SfaNI GCATC 4 cut(s) 649, 808, 939, 1024
SfuI TTCGAA 1 cut(s) 891
SinI GGWCC 1 cut(s) 64
SmiI ATTTAAAT 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
SphI GCATGC 1 cut(s) 365
SsiI CCGC 2 cut(s) 328, 896
SspI AATATT 1 cut(s) 1085
SspMI CTAG 3 cut(s) 233, 282, 666
StyD4I CCNGG 2 cut(s) 183, 237
SwaI ATTTAAAT 1 cut(s) 120
TaaI ACNGT 2 cut(s) 208, 1057
TaiI ACGT 4 cut(s) 180, 453, 679, 841
TaqI TCGA 5 cut(s) 475, 573, 578, 891, 1104
TatI WGTACW 2 cut(s) 203, 936
TfiI GAWTC 2 cut(s) 548, 575
Tru1I TTAA 6 cut(s) 20, 119, 180, 249, 288, 1159
Tru9I TTAA 6 cut(s) 20, 119, 180, 249, 288, 1159
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 2 cut(s) 458, 1031
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 2 cut(s) 458, 1031
TspDTI ATGAA 8 cut(s) 20, 138, 210, 234, 342, 437, 1065, 1127
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 236
XapI RAATTY 2 cut(s) 887, 927
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XspI CTAG 3 cut(s) 233, 282, 666
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.