MD10G1278800.v1.1
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005527
Physical Location & Seq
Forward (+)
36984346 .. 36988192
3847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1278800.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGATGAGCTAGAATTTGGTTATCAGGAAGCTGAGAAGAGTAACGAGATCAGCGAGGAACAAGTAGAAAACGGATCAGTGGGATCGTCCCAGAAATTCTCATCCTTCGATTTGAATGAAGAAGCAAGTAGTGACAATGAAGAAAGAGGCCAAGGGAATAAAGCTGCAGATAATGATATCTCTAGTGAAGGAAATGAAGGGCGGAGAGTTAAAGTAAGGCAATATGTGAGATCGAAAATGCCTCGTCTTCGGTGGACACCTCAACTCCATCTTTCTTTTGTGCACGCTGTTGAAAGACTGGGTGGCCAAGATAGAGCAACTCCTAAATTAGTTCTTAAGCTAATGAATGTGAGAGGACTTAGCATTTCTCATGTAAAGAGTCACTTGCAGATGTATAGAAGTAAGAAGCTTGATGGGAATGGCCAAGTATTATCTGAAAACCACGAATCCAAGCATGGAAGGGATTACATATCTTGCATGGTGCACCAAACAACCAACCAGCCTCGTCGACATGTATACTTTAGAATGGACAACGGTGGCATCGTTGTAGCAACAAATCCACACGACTGTCATAGCCAATCACAGCCAGATTTTAAAGCTAGCTCAAGGTCAAGTTCTCTACTAAACAAAGAACTCGCAAAAGGCAACAACTCGAACAAATCTATAGATACAATCTTGCAAACTTTACCGATAAACCCTAGCAAGTTTCTTGAGGAAAGGCCTCCATTTGAAACGATTAACAAACAACAATGGAGGGCTAAAAGAAGACCAGCTGCCAAAATCAATTGGTCAGATAATCGTCATGGCTCCCAATCTGACCATCTAATGCATCATATGCACACCACACCAAGGTTGATAGGGGAATCTCACAATTTAAGACCACCAAACTGGCACCTTGGAGAGAGCAGAAACGTAAGGCAATTTCCATCAAACACACACAATTCTATCACCAATTCCAGTTGCTACAAAACCGAATGCAAGTCGCCATTTAGGTTTGAGCTGAATGAAGAAAATTTTTCTAAGTACAAAGAATGGATGCCACAACTGGAATTGGGTCTGGGCCAAAGAGTTGAGAATTATGAGGAAAAGAAGATTCTTTCCAGACATCGTATGGTTAGTGAAGATCATTATGGAACTGGGCAAAGAGTTAAACAGGAAATTAGCACTAAGCTTTCTCTTTCTCTTTAA

Protein Analysis

396

Amino Acids

45.47

Weight (kDa)

9.17

Isoelectric Point (pI)

62.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 82 - 133 3e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 891
AccI GTMKAC 2 cut(s) 508, 516
AciI CCGC 1 cut(s) 202
AclWI GGATC 2 cut(s) 82, 91
AcoI YGGCCR 2 cut(s) 304, 421
AcsI RAATTY 3 cut(s) 14, 95, 1012
AfaI GTAC 1 cut(s) 1025
AfiI CCNNNNNNNGG 1 cut(s) 849
AflII CTTAAG 1 cut(s) 335
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 3 cut(s) 115, 293, 731
Alw21I GWGCWC 2 cut(s) 285, 486
Alw44I GTGCAC 2 cut(s) 281, 482
AlwI GGATC 2 cut(s) 82, 91
AoxI GGCC 5 cut(s) 148, 304, 421, 719, 1060
ApaLI GTGCAC 2 cut(s) 281, 482
ApeKI GCWGC 2 cut(s) 164, 773
ApoI RAATTY 3 cut(s) 14, 95, 1012
AspS9I GGNCC 1 cut(s) 1060
AsuHPI GGTGA 1 cut(s) 940
AsuNHI GCTAGC 1 cut(s) 599
BaeGI GKGCMC 2 cut(s) 285, 486
BalI TGGCCA 2 cut(s) 306, 423
BanI GGYRCC 1 cut(s) 891
BbsI GAAGAC 2 cut(s) 239, 772
Bbv12I GWGCWC 2 cut(s) 285, 486
BbvI GCAGC 2 cut(s) 151, 760
BccI CCATC 4 cut(s) 276, 407, 828, 934
BfaI CTAG 4 cut(s) 11, 183, 600, 699
BfmI CTRYAG 2 cut(s) 165, 663
BfrI CTTAAG 1 cut(s) 335
BisI GCNGC 2 cut(s) 165, 774
BlsI GCNGC 2 cut(s) 166, 775
BmgT120I GGNCC 1 cut(s) 1060
BmiI GGNNCC 2 cut(s) 808, 893
BmrI ACTGGG 2 cut(s) 308, 1146
BmsI GCATC 3 cut(s) 549, 838, 1026
BmtI GCTAGC 1 cut(s) 603
BmuI ACTGGG 2 cut(s) 308, 1146
BpiI GAAGAC 2 cut(s) 239, 772
BpuEI CTTGAG 2 cut(s) 589, 731
BsaJI CCNNGG 3 cut(s) 151, 848, 895
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse1I ACTGG 5 cut(s) 303, 893, 957, 1050, 1141
BseDI CCNNGG 3 cut(s) 151, 848, 895
BseGI GGATG 3 cut(s) 10, 101, 1041
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 5 cut(s) 303, 893, 957, 1050, 1141
BseSI GKGCMC 2 cut(s) 285, 486
BseXI GCAGC 2 cut(s) 151, 760
BshFI GGCC 5 cut(s) 150, 306, 423, 721, 1062
BshNI GGYRCC 1 cut(s) 891
BsiHKAI GWGCWC 2 cut(s) 285, 486
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 1 cut(s) 849
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 980
BsnI GGCC 5 cut(s) 150, 306, 423, 721, 1062
Bsp1286I GDGCHC 2 cut(s) 285, 486
Bsp143I GATC 5 cut(s) 48, 74, 83, 230, 1123
BspACI CCGC 1 cut(s) 202
BspANI GGCC 5 cut(s) 150, 306, 423, 721, 1062
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 2 cut(s) 808, 893
BspMAI CTGCAG 1 cut(s) 169
BspOI GCTAGC 1 cut(s) 603
BspPI GGATC 2 cut(s) 82, 91
BspT107I GGYRCC 1 cut(s) 891
BspTI CTTAAG 1 cut(s) 335
BsrI ACTGG 5 cut(s) 303, 893, 957, 1050, 1141
BssECI CCNNGG 3 cut(s) 151, 848, 895
BssMI GATC 5 cut(s) 48, 74, 83, 230, 1123
BssNAI GTATAC 1 cut(s) 517
BssT1I CCWWGG 3 cut(s) 151, 848, 895
Bst1107I GTATAC 1 cut(s) 517
Bst4CI ACNGT 2 cut(s) 536, 569
Bst6I CTCTTC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 335
BstAPI GCANNNNNTGC 1 cut(s) 835
BstC8I GCNNGC 2 cut(s) 285, 601
BstDEI CTNAG 4 cut(s) 33, 359, 1020, 1167
BstF5I GGATG 3 cut(s) 10, 101, 1041
BstKTI GATC 5 cut(s) 51, 77, 86, 233, 1126
BstMBI GATC 5 cut(s) 48, 74, 83, 230, 1123
BstMWI GCNNNNNNNGC 1 cut(s) 835
BstNSI RCATGY 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 165, 663
BstSLI GKGCMC 2 cut(s) 285, 486
BstV1I GCAGC 2 cut(s) 151, 760
BstV2I GAAGAC 2 cut(s) 239, 772
BstXI CCANNNNNNTGG 1 cut(s) 888
BstZ17I GTATAC 1 cut(s) 517
BsuRI GGCC 5 cut(s) 150, 306, 423, 721, 1062
BtsCI GGATG 3 cut(s) 10, 101, 1041
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 285, 601
Cfr13I GGNCC 1 cut(s) 1060
Csp6I GTAC 1 cut(s) 1024
CviAII CATG 5 cut(s) 371, 455, 478, 512, 803
CviQI GTAC 1 cut(s) 1024
DdeI CTNAG 4 cut(s) 33, 359, 1020, 1167
DpnI GATC 5 cut(s) 50, 76, 85, 232, 1125
DpnII GATC 5 cut(s) 48, 74, 83, 230, 1123
DraI TTTAAA 1 cut(s) 595
EaeI YGGCCR 2 cut(s) 304, 421
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 217
Eco130I CCWWGG 3 cut(s) 151, 848, 895
Eco147I AGGCCT 1 cut(s) 721
Eco32I GATATC 1 cut(s) 178
EcoRV GATATC 1 cut(s) 178
EcoT14I CCWWGG 3 cut(s) 151, 848, 895
EcoT22I ATGCAT 1 cut(s) 831
ErhI CCWWGG 3 cut(s) 151, 848, 895
FaeI CATG 5 cut(s) 374, 458, 481, 515, 806
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 1 cut(s) 73
FatI CATG 5 cut(s) 370, 454, 477, 511, 802
FauNDI CATATG 1 cut(s) 834
FblI GTMKAC 2 cut(s) 508, 516
Fnu4HI GCNGC 2 cut(s) 165, 774
FokI GGATG 3 cut(s) 17, 88, 1048
Fsp4HI GCNGC 2 cut(s) 165, 774
FspBI CTAG 4 cut(s) 11, 183, 600, 699
GluI GCNGC 2 cut(s) 165, 774
HaeIII GGCC 5 cut(s) 150, 306, 423, 721, 1062
Hin1II CATG 5 cut(s) 374, 458, 481, 515, 806
HincII GTYRAC 1 cut(s) 509
HindII GTYRAC 1 cut(s) 509
HindIII AAGCTT 2 cut(s) 407, 1169
HinfI GANTC 4 cut(s) 379, 446, 863, 1093
HphI GGTGA 1 cut(s) 940
Hpy166II GTNNAC 5 cut(s) 255, 283, 484, 509, 517
Hpy188I TCNGA 3 cut(s) 436, 793, 817
Hpy188III TCNNGA 3 cut(s) 26, 710, 1101
Hpy8I GTNNAC 5 cut(s) 255, 283, 484, 509, 517
Hpy99I CGWCG 1 cut(s) 510
HpyAV CCTTC 4 cut(s) 115, 182, 191, 453
HpyCH4III ACNGT 2 cut(s) 536, 569
HpyCH4IV ACGT 1 cut(s) 912
HpyCH4V TGCA 9 cut(s) 167, 283, 388, 477, 484, 679, 829, 838, 978
HpyF10VI GCNNNNNNNGC 1 cut(s) 835
HpyF3I CTNAG 4 cut(s) 33, 359, 1020, 1167
HpySE526I ACGT 1 cut(s) 912
Hsp92II CATG 5 cut(s) 374, 458, 481, 515, 806
Kzo9I GATC 5 cut(s) 48, 74, 83, 230, 1123
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 2 cut(s) 151, 760
LweI GCATC 3 cut(s) 549, 838, 1026
MaeI CTAG 4 cut(s) 11, 183, 600, 699
MaeII ACGT 1 cut(s) 912
MaeIII GTNAC 3 cut(s) 41, 131, 380
MalI GATC 5 cut(s) 50, 76, 85, 232, 1125
MboI GATC 5 cut(s) 48, 74, 83, 230, 1123
MboII GAAGA 8 cut(s) 49, 131, 152, 239, 777, 1019, 1102, 1133
MfeI CAATTG 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 285, 486
MlsI TGGCCA 2 cut(s) 306, 423
MluNI TGGCCA 2 cut(s) 306, 423
MlyI GAGTC 1 cut(s) 388
Mox20I TGGCCA 2 cut(s) 306, 423
Mph1103I ATGCAT 1 cut(s) 831
MscI TGGCCA 2 cut(s) 306, 423
MseI TTAA 7 cut(s) 210, 336, 594, 738, 875, 1149, 1186
Msp20I TGGCCA 2 cut(s) 306, 423
MspA1I CMGCKG 1 cut(s) 773
MspCI CTTAAG 1 cut(s) 335
MunI CAATTG 1 cut(s) 784
Mva1269I GAATGC 1 cut(s) 980
MwoI GCNNNNNNNGC 1 cut(s) 835
NdeI CATATG 1 cut(s) 834
NdeII GATC 5 cut(s) 48, 74, 83, 230, 1123
NheI GCTAGC 1 cut(s) 599
NlaIII CATG 5 cut(s) 374, 458, 481, 515, 806
NlaIV GGNNCC 2 cut(s) 808, 893
NmuCI GTSAC 2 cut(s) 131, 380
NsiI ATGCAT 1 cut(s) 831
NspI RCATGY 1 cut(s) 515
PceI AGGCCT 1 cut(s) 721
PciI ACATGT 1 cut(s) 511
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 540
PctI GAATGC 1 cut(s) 980
PfeI GAWTC 3 cut(s) 446, 863, 1093
PkrI GCNGC 2 cut(s) 166, 775
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
PscI ACATGT 1 cut(s) 511
PspN4I GGNNCC 2 cut(s) 808, 893
PspPI GGNCC 1 cut(s) 1060
PstI CTGCAG 1 cut(s) 169
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
SalI GTCGAC 1 cut(s) 507
SaqAI TTAA 7 cut(s) 210, 336, 594, 738, 875, 1149, 1186
SatI GCNGC 2 cut(s) 165, 774
Sau3AI GATC 5 cut(s) 48, 74, 83, 230, 1123
Sau96I GGNCC 1 cut(s) 1060
SchI GAGTC 1 cut(s) 388
SduI GDGCHC 2 cut(s) 285, 486
SfaNI GCATC 3 cut(s) 549, 838, 1026
SfcI CTRYAG 2 cut(s) 165, 663
SmlI CTYRAG 3 cut(s) 335, 604, 710
SmoI CTYRAG 3 cut(s) 335, 604, 710
SseBI AGGCCT 1 cut(s) 721
SsiI CCGC 1 cut(s) 202
SspMI CTAG 4 cut(s) 11, 183, 600, 699
StuI AGGCCT 1 cut(s) 721
StyI CCWWGG 3 cut(s) 151, 848, 895
TaaI ACNGT 2 cut(s) 536, 569
TaiI ACGT 1 cut(s) 915
TaqI TCGA 4 cut(s) 108, 233, 508, 653
TatI WGTACW 1 cut(s) 1023
TfiI GAWTC 3 cut(s) 446, 863, 1093
Tru1I TTAA 7 cut(s) 210, 336, 594, 738, 875, 1149, 1186
Tru9I TTAA 7 cut(s) 210, 336, 594, 738, 875, 1149, 1186
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 2 cut(s) 131, 380
TseI GCWGC 2 cut(s) 164, 773
Tsp45I GTSAC 2 cut(s) 131, 380
TspDTI ATGAA 5 cut(s) 132, 153, 210, 359, 1020
TspGWI ACGGA 1 cut(s) 87
TspRI CASTG 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 335
VneI GTGCAC 2 cut(s) 281, 482
XapI RAATTY 3 cut(s) 14, 95, 1012
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XmiI GTMKAC 2 cut(s) 508, 516
XspI CTAG 4 cut(s) 11, 183, 600, 699
Zsp2I ATGCAT 1 cut(s) 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.