Rroxscaffold_1G00065390
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005527
Physical Location & Seq
Forward (+)
87058696 .. 87061937
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00065390.1

Sequence Viewer

Length: 1152 bp
ATGATGAATGAGCTGGAGTTAATTGGTGATCAGAAAGAAAAGACTGACAAAAATATTAGTGAGGACCAAGCAGAAAACATATCGGTGGGTTCTTCCCAGAAATGCTCATGCTTTGATTTAAATGAAGAAGCTAGCGATAACAGCGACAATGATATTAGCAATGCCAACGTTAACCTGGCTGATCATGAACTTAGTACAGTTAGCAATGATGAAAGAAGAGCCCTAGCCCAGGGAAACATTAATAGTAATGCAGCTAATATTAGTAATAGCTCTAGGGTTAAAGTAAGGCCATATGTACGATCCAAACTACCTCGTCTGCGGTGGACACCCCAACTTCATCTTTCATTTTTGAATGCTTTGGAATTACTGGGAGGGCATGAGAGAGCAACTCCCAAGTTGGTTCTTCAGCTAATGAATGTGAGAGGACTAAGCATTTCCCACGTAAAGAGTCACTTGCAGATGTATCGAAGTAAGAAGCTTGATAGGGATGGTCAAGTAGTATCTGATAAGAAAACTTACGACTACACGGATGGAAGAGAATCAAGGGATCACATACCTAACTTCGTTTCGAGGTCAAGTTCTCTGACAAAGAAAGAAGTCGTACAAGGCAACAATTTGAACAAAGCTTTGCATCATACAAGCACTCCCATAAACCCTAGCCGATTACACGAGGAAAAGAGGTGGCCTCCATTTCAAAGCATTAGCAAAAGCTGGAGGGCCAGAAGATTTGCTTCCAACAATATCAATTGGTTAAATAGTCATAATGGCTCATCCCAATCTGATATAATGCATCATATGATTAGCAACAGACCAACAGATCATGATCTACGTTGTTGGAACCTTGAAGGTCATAGCAATTCAAGTAGCTACAAACAAGAATTCGAACCGCCATTTAGGCTTGAGTGGAACAAGGAAAAAATTTTGAAGTACAAGGAATGGATACCTGATTTGAAGTTGAGTCTGCAGAGCCAAAGAGTTTGGAATGAGATCAATGACGAGACTCAGCATCATCAGACGATTAGGCATGCAGAAGAACATTATGAAACTGTGCAAGGAGTTATAGAGACTTCACAACATTACAAAGATCATCAATTCGAGGACATGAACAGCAAACAAGAAACTATTAGCACCAAGCTTTCCCTTTCTCTTTAA

Protein Analysis

383

Amino Acids

44.3

Weight (kDa)

7.06

Isoelectric Point (pI)

57.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 105 - 156 2.5e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 319, 887
AclI AACGTT 1 cut(s) 168
AclWI GGATC 2 cut(s) 294, 555
AcsI RAATTY 2 cut(s) 878, 918
AcuI CTGAAG 1 cut(s) 389
AfaI GTAC 4 cut(s) 196, 297, 603, 929
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 7 cut(s) 352, 619, 695, 845, 861, 925, 952
AjnI CCWGG 2 cut(s) 174, 228
Alw26I GTCTC 2 cut(s) 992, 1058
AlwI GGATC 2 cut(s) 294, 555
AoxI GGCC 3 cut(s) 287, 683, 717
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 2 cut(s) 878, 918
AseI ATTAAT 1 cut(s) 240
AspS9I GGNCC 2 cut(s) 64, 717
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 882
AsuNHI GCTAGC 1 cut(s) 131
AvaII GGWCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 223
BauI CACGAG 1 cut(s) 668
BbvI GCAGC 1 cut(s) 263
BccI CCATC 2 cut(s) 482, 524
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 2 cut(s) 176, 230
BciVI GTATCC 1 cut(s) 933
BclI TGATCA 2 cut(s) 28, 181
BcoDI GTCTC 2 cut(s) 992, 1058
BfaI CTAG 4 cut(s) 132, 224, 273, 657
BfmI CTRYAG 1 cut(s) 962
BfuI GTATCC 1 cut(s) 933
BglI GCCNNNNNGGC 1 cut(s) 895
BisI GCNGC 1 cut(s) 252
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 2 cut(s) 176, 230
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 717
BmiI GGNNCC 1 cut(s) 839
BmrFI CCNGG 2 cut(s) 176, 230
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 3 cut(s) 640, 799, 1015
BmtI GCTAGC 1 cut(s) 135
BmuI ACTGGG 1 cut(s) 377
BplI GAGNNNNNCTC 4 cut(s) 373, 405, 670, 702
BpmI CTGGAG 2 cut(s) 35, 733
Bpu14I TTCGAA 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 920
BsaAI YACGTR 1 cut(s) 442
BsaBI GATNNNNATC 1 cut(s) 822
BsaJI CCNNGG 2 cut(s) 228, 229
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 372
Bse3DI GCAATG 2 cut(s) 166, 211
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 2 cut(s) 176, 230
BseDI CCNNGG 2 cut(s) 228, 229
BseGI GGATG 3 cut(s) 493, 535, 770
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 2 cut(s) 166, 211
BseMII CTCAG 1 cut(s) 1016
BseNI ACTGG 1 cut(s) 372
BseXI GCAGC 1 cut(s) 263
BshFI GGCC 3 cut(s) 289, 685, 719
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 2 cut(s) 992, 1058
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 3 cut(s) 289, 685, 719
Bsp119I TTCGAA 1 cut(s) 882
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
BspACI CCGC 2 cut(s) 319, 887
BspANI GGCC 3 cut(s) 289, 685, 719
BspCNI CTCAG 1 cut(s) 1015
BspHI TCATGA 2 cut(s) 184, 820
BspLI GGNNCC 1 cut(s) 839
BspMAI CTGCAG 1 cut(s) 966
BspOI GCTAGC 1 cut(s) 135
BspPI GGATC 2 cut(s) 294, 555
BspQI GCTCTTC 1 cut(s) 211
BspT104I TTCGAA 1 cut(s) 882
BsrDI GCAATG 2 cut(s) 166, 211
BsrI ACTGG 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 228, 229
BssMI GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
BssSI CACGAG 1 cut(s) 668
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 2 cut(s) 176, 230
Bst4CI ACNGT 2 cut(s) 199, 1048
Bst6I CTCTTC 2 cut(s) 211, 529
BstBAI YACGTR 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 882
BstC8I GCNNGC 2 cut(s) 133, 1026
BstDEI CTNAG 3 cut(s) 191, 428, 1002
BstENI CCTNNNNNAGG 1 cut(s) 227
BstF5I GGATG 3 cut(s) 493, 535, 770
BstKTI GATC 8 cut(s) 31, 184, 302, 550, 820, 826, 990, 1087
BstMAI GTCTC 2 cut(s) 992, 1058
BstMBI GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 141, 895
BstNI CCWGG 2 cut(s) 176, 230
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 2 cut(s) 174, 228
BstSFI CTRYAG 1 cut(s) 962
BstV1I GCAGC 1 cut(s) 263
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 3 cut(s) 289, 685, 719
BtsCI GGATG 3 cut(s) 493, 535, 770
Cac8I GCNNGC 2 cut(s) 133, 1026
CciI TCATGA 2 cut(s) 184, 820
Cfr13I GGNCC 2 cut(s) 64, 717
Csp6I GTAC 4 cut(s) 195, 296, 602, 928
CviAII CATG 6 cut(s) 108, 185, 377, 821, 1025, 1102
CviQI GTAC 4 cut(s) 195, 296, 602, 928
DdeI CTNAG 3 cut(s) 191, 428, 1002
DpnI GATC 8 cut(s) 30, 183, 301, 549, 819, 825, 989, 1086
DpnII GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
DraI TTTAAA 1 cut(s) 120
Eam1104I CTCTTC 2 cut(s) 211, 529
EarI CTCTTC 2 cut(s) 211, 529
Eco24I GRGCYC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 389
EcoNI CCTNNNNNAGG 1 cut(s) 227
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 2 cut(s) 174, 228
EcoT22I ATGCAT 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 6 cut(s) 111, 188, 380, 824, 1028, 1105
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 715, 747
FatI CATG 6 cut(s) 107, 184, 376, 820, 1024, 1101
FauNDI CATATG 2 cut(s) 292, 795
FbaI TGATCA 2 cut(s) 28, 181
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 3 cut(s) 500, 542, 757
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 4 cut(s) 132, 224, 273, 657
GluI GCNGC 1 cut(s) 252
GsuI CTGGAG 2 cut(s) 35, 733
HaeIII GGCC 3 cut(s) 289, 685, 719
Hin1II CATG 6 cut(s) 111, 188, 380, 824, 1028, 1105
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 3 cut(s) 476, 624, 1133
HinfI GANTC 4 cut(s) 448, 539, 958, 1000
HpaI GTTAAC 1 cut(s) 172
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 2 cut(s) 172, 324
Hpy188I TCNGA 5 cut(s) 33, 505, 585, 781, 1014
Hpy188III TCNNGA 2 cut(s) 185, 821
Hpy8I GTNNAC 2 cut(s) 172, 324
HpyAV CCTTC 1 cut(s) 839
HpyCH4III ACNGT 2 cut(s) 199, 1048
HpyCH4IV ACGT 3 cut(s) 168, 441, 829
HpyCH4V TGCA 7 cut(s) 251, 457, 631, 790, 964, 1028, 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 141, 895
HpyF3I CTNAG 3 cut(s) 191, 428, 1002
HpySE526I ACGT 3 cut(s) 168, 441, 829
Hsp92II CATG 6 cut(s) 111, 188, 380, 824, 1028, 1105
Ksp22I TGATCA 2 cut(s) 28, 181
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
LguI GCTCTTC 1 cut(s) 211
LpnPI CCDG 9 cut(s) 110, 161, 188, 215, 242, 353, 697, 733, 957
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 3 cut(s) 640, 799, 1015
MaeI CTAG 4 cut(s) 132, 224, 273, 657
MaeII ACGT 3 cut(s) 168, 441, 829
MaeIII GTNAC 1 cut(s) 449
MalI GATC 8 cut(s) 30, 183, 301, 549, 819, 825, 989, 1086
MboI GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
MboII GAAGA 7 cut(s) 84, 137, 228, 395, 546, 735, 1043
MfeI CAATTG 1 cut(s) 745
MhlI GDGCHC 1 cut(s) 223
MluCI AATT 8 cut(s) 21, 362, 613, 745, 856, 878, 918, 1091
MlyI GAGTC 3 cut(s) 457, 967, 994
MmeI TCCRAC 2 cut(s) 759, 815
Mph1103I ATGCAT 1 cut(s) 792
MseI TTAA 7 cut(s) 20, 119, 171, 240, 279, 752, 1150
MslI CAYNNNNRTG 1 cut(s) 83
MspR9I CCNGG 2 cut(s) 176, 230
MunI CAATTG 1 cut(s) 745
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 2 cut(s) 176, 230
MwoI GCNNNNNNNGC 2 cut(s) 141, 895
NdeI CATATG 2 cut(s) 292, 795
NdeII GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
NheI GCTAGC 1 cut(s) 131
NlaIII CATG 6 cut(s) 111, 188, 380, 824, 1028, 1105
NlaIV GGNNCC 1 cut(s) 839
NmuCI GTSAC 1 cut(s) 449
NsiI ATGCAT 1 cut(s) 792
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 1 cut(s) 882
PaeI GCATGC 1 cut(s) 1028
PagI TCATGA 2 cut(s) 184, 820
PasI CCCWGGG 1 cut(s) 229
PciSI GCTCTTC 1 cut(s) 211
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 539
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 3 cut(s) 456, 966, 994
PpsI GAGTC 3 cut(s) 456, 966, 994
Ppu21I YACGTR 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 168
Psp6I CCWGG 2 cut(s) 174, 228
PspGI CCWGG 2 cut(s) 174, 228
PspN4I GGNNCC 1 cut(s) 839
PspPI GGNCC 2 cut(s) 64, 717
PstI CTGCAG 1 cut(s) 966
RsaI GTAC 4 cut(s) 196, 297, 603, 929
RsaNI GTAC 4 cut(s) 195, 296, 602, 928
RseI CAYNNNNRTG 1 cut(s) 83
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 7 cut(s) 20, 119, 171, 240, 279, 752, 1150
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 8 cut(s) 28, 181, 299, 547, 817, 823, 987, 1084
Sau96I GGNCC 2 cut(s) 64, 717
SchI GAGTC 3 cut(s) 457, 967, 994
ScrFI CCNGG 2 cut(s) 176, 230
SduI GDGCHC 1 cut(s) 223
SfaNI GCATC 3 cut(s) 640, 799, 1015
SfcI CTRYAG 1 cut(s) 962
SfuI TTCGAA 1 cut(s) 882
SinI GGWCC 1 cut(s) 64
SmiI ATTTAAAT 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
SphI GCATGC 1 cut(s) 1028
Sse9I AATT 8 cut(s) 21, 362, 613, 745, 856, 878, 918, 1091
SsiI CCGC 2 cut(s) 319, 887
SspI AATATT 2 cut(s) 55, 259
SspMI CTAG 4 cut(s) 132, 224, 273, 657
StyD4I CCNGG 2 cut(s) 174, 228
SwaI ATTTAAAT 1 cut(s) 120
TaaI ACNGT 2 cut(s) 199, 1048
TaiI ACGT 3 cut(s) 171, 444, 832
TaqI TCGA 4 cut(s) 466, 569, 882, 1095
TasI AATT 8 cut(s) 21, 362, 613, 745, 856, 878, 918, 1091
TatI WGTACW 2 cut(s) 194, 927
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 7 cut(s) 20, 119, 171, 240, 279, 752, 1150
Tru9I TTAA 7 cut(s) 20, 119, 171, 240, 279, 752, 1150
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 9 cut(s) 20, 138, 201, 225, 326, 333, 428, 1056, 1118
TspGWI ACGGA 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 64
VspI ATTAAT 1 cut(s) 240
XagI CCTNNNNNAGG 1 cut(s) 227
XapI RAATTY 2 cut(s) 878, 918
XceI RCATGY 1 cut(s) 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 4 cut(s) 132, 224, 273, 657
Zsp2I ATGCAT 1 cut(s) 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.