MD05G1324100.v1.1

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002149
Physical Location & Seq
Forward (+)
45096271 .. 45100619
4349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1324100.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAACCAGAAGGCTAGCGTTTCTAAAGAGCTTAATGCAAAACACAGAAAGAGATTGGAGGGTCTTCTTAAACTACAAGAGAACAGAGAATGCGCAGACTGCAAAACCAAAGCTCCACGATGGGCTAGCGTGAATCTTGGTATCTTCATATGCATGCAATGTTCAGGGATCCACAGGAGTCTTGGGGTACATATATCACAGGTGAGATCTGCCACTTTGGATACATGGCTCCCAGAGCAGGTTGCGTTTATCCAATCTATGGGAAACGATAAAGCGAATAGCTATTGGGAAGCCGAGTTGCCTCCTAAGTATGACAGAGTTGGAATCGAGAATTTCATTCGAGCAAAGTATGAACACAAAAAATGGGTTCCTGGGGATGGAAAAGCGAAATCACCTGCGAGGGTGAGTGAGGCAATGACTTCCGTTTATAGAGCGAGACCTGAAACTAGTACTCAACATGTCCATTTGAAAAAGATGGAACAGTTTTCTGAGGAGCAGAAAGGTGCTCGTACACACGGTACAAATAACTTGACAACGCAGCAGATCAGTCATGATCCAAAACCACAAGCACTTGTGCAGAAACCAAAACAACAAGAACTTGTGCAGAAACCAAAACAACAAGAACTTGTGCAGAAACCAAAACAACAAGAACTTGTTCAGAAACCTAAACCACAAGAACTTGCACAGGAACCAAAACCAGTAGTCTTGAAAGTTGAACCAGCAAGTGAGGCGGGTAGTGTTAGGCCCGTTTCAGTACCAGTTAAAGTGGATTATGCTACTCATTTGTTCAATTTGCTATCCATGGAGGAAGCTGTAGAAAATGACTCAGTGATATCTGCCAACACGAATACATTGGCTGGTAACCACGTTGAAACAAAGTTGACAGAAGAGAAAAGTAAAAGGCCAGAATCATCCGAAAGCAAGATTCAACCAAAATATGGTGGTCTCGAGGATTTATTTGTACATCCAACCCCCGACACTCAACCGTTCTCAATGAAACCTCAGAATTATGTGCAGAATGACATTATGAACTTATTCGAGAAGTCCAGTGTGGCTTCTCCATCCTCTGTGCATCAACAGCAACTTGCAATGCCATACCAACAACAGGCATTACCCATGGCGACTGTGGCGAAATCTAGTGGATCCCATTCCGTTCCTGCAAACGTACAACCTGGCTCCCATTCTATCCAGCACTTACCCGCCCGAAATGGGCAAAGCATTGGTCGCCAAGTTCCTGGATACCTGGTACCTGTTCATCCTCAACATTATCCTCAGATTGGAAACAGCTATCATACACGTCCGGTAGAGAATACAGTTCCTTTCCAAACATCAAGCATGTACAGGACGAGGCCAGTAACACCAATTAATGGTGTCACAAACATCAGAGCTAATGGATTTTCATCAGCGTCACCAATATCTTCTTTCCCTTCAACTAAGTCAGGAAGCGATTTCGATTTCTCATCATTAACTCAAGGATTGTTTACCAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

498

Amino Acids

55.34

Weight (kDa)

9.44

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 5.7e-41 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
Acc16I TGCGCA 1 cut(s) 94
Acc36I ACCTGC 2 cut(s) 229, 403
Acc65I GGTACC 1 cut(s) 1246
AccB1I GGYRCC 1 cut(s) 1246
AccB7I CCANNNNNTGG 3 cut(s) 259, 938, 1367
AciI CCGC 2 cut(s) 731, 1200
AclWI GGATC 5 cut(s) 163, 176, 548, 1137, 1150
AcsI RAATTY 1 cut(s) 331
AfaI GTAC 9 cut(s) 189, 451, 511, 520, 756, 963, 1167, 1248, 1340
AfiI CCNNNNNNNGG 8 cut(s) 238, 259, 377, 938, 1105, 1209, 1277, 1367
AflIII ACRYGT 2 cut(s) 457, 1295
AgsI TTSAA 7 cut(s) 469, 709, 716, 790, 872, 929, 1431
AhlI ACTAGT 1 cut(s) 446
AjiI CACGTC 1 cut(s) 1298
AjnI CCWGG 4 cut(s) 370, 1171, 1234, 1242
AluBI AGCT 6 cut(s) 31, 113, 282, 812, 1287, 1388
AluI AGCT 6 cut(s) 31, 113, 282, 812, 1287, 1388
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 2 cut(s) 430, 950
AlwI GGATC 5 cut(s) 163, 176, 548, 1137, 1150
Ama87I CYCGRG 1 cut(s) 947
AoxI GGCC 3 cut(s) 743, 902, 1349
ApeKI GCWGC 1 cut(s) 538
ApoI RAATTY 1 cut(s) 331
ArsI GACNNNNNNTTYG 2 cut(s) 1207, 1239
AseI ATTAAT 1 cut(s) 1365
Asp700I GAANNNNTTC 2 cut(s) 654, 1034
Asp718I GGTACC 1 cut(s) 1246
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 744
AsuHPI GGTGA 4 cut(s) 214, 384, 415, 1401
AsuNHI GCTAGC 2 cut(s) 14, 125
AvaI CYCGRG 1 cut(s) 947
BamHI GGATCC 2 cut(s) 168, 1142
BanI GGYRCC 1 cut(s) 1246
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 508
BbvI GCAGC 1 cut(s) 550
BccI CCATC 4 cut(s) 114, 371, 469, 1069
BciT130I CCWGG 4 cut(s) 372, 1173, 1236, 1244
BciVI GTATCC 2 cut(s) 214, 1232
BcoDI GTCTC 2 cut(s) 430, 950
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 15, 126, 447, 1137
BfmI CTRYAG 1 cut(s) 813
BfuAI ACCTGC 2 cut(s) 229, 403
BfuI GTATCC 2 cut(s) 214, 1232
BglII AGATCT 1 cut(s) 206
BisI GCNGC 1 cut(s) 539
BlsI GCNGC 1 cut(s) 540
BmcAI AGTACT 1 cut(s) 451
Bme1390I CCNGG 4 cut(s) 372, 1173, 1236, 1244
BmeT110I CYCGRG 1 cut(s) 947
BmgBI CACGTC 1 cut(s) 1298
BmgT120I GGNCC 1 cut(s) 744
BmiI GGNNCC 7 cut(s) 170, 230, 369, 690, 1144, 1177, 1248
BmrFI CCNGG 4 cut(s) 372, 1173, 1236, 1244
BmsI GCATC 1 cut(s) 1081
BmtI GCTAGC 2 cut(s) 18, 129
BpiI GAAGAC 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 1455
BsaBI GATNNNNATC 1 cut(s) 1399
BsaI GGTCTC 2 cut(s) 430, 950
BsaJI CCNNGG 3 cut(s) 371, 801, 1116
BsaWI WCCGGW 1 cut(s) 1300
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 8 cut(s) 238, 259, 377, 938, 1105, 1209, 1277, 1367
Bse1I ACTGG 4 cut(s) 698, 758, 1047, 1352
Bse3DI GCAATG 3 cut(s) 164, 420, 1095
Bse8I GATNNNNATC 1 cut(s) 1399
BseBI CCWGG 4 cut(s) 372, 1173, 1236, 1244
BseDI CCNNGG 3 cut(s) 371, 801, 1116
BseGI GGATG 5 cut(s) 382, 911, 964, 1061, 1255
BseJI GATNNNNATC 1 cut(s) 1399
BseLI CCNNNNNNNGG 8 cut(s) 238, 259, 377, 938, 1105, 1209, 1277, 1367
BseMI GCAATG 3 cut(s) 164, 420, 1095
BseMII CTCAG 4 cut(s) 480, 840, 1016, 1286
BseNI ACTGG 4 cut(s) 698, 758, 1047, 1352
BseRI GAGGAG 1 cut(s) 506
BseXI GCAGC 1 cut(s) 550
BsgI GTGCAG 4 cut(s) 596, 623, 650, 1034
BshFI GGCC 3 cut(s) 745, 904, 1351
BshNI GGYRCC 1 cut(s) 1246
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 1 cut(s) 947
BsiSI CCGG 1 cut(s) 1301
BslI CCNNNNNNNGG 8 cut(s) 238, 259, 377, 938, 1105, 1209, 1277, 1367
BsmAI GTCTC 2 cut(s) 430, 950
BsmI GAATGC 1 cut(s) 95
BsnI GGCC 3 cut(s) 745, 904, 1351
Bso31I GGTCTC 2 cut(s) 430, 950
BsoBI CYCGRG 1 cut(s) 947
Bsp1286I GDGCHC 1 cut(s) 508
Bsp1407I TGTACA 2 cut(s) 961, 1338
Bsp143I GATC 5 cut(s) 168, 206, 543, 553, 1142
Bsp19I CCATGG 2 cut(s) 801, 1116
BspACI CCGC 2 cut(s) 731, 1200
BspANI GGCC 3 cut(s) 745, 904, 1351
BspCNI CTCAG 4 cut(s) 481, 839, 1015, 1285
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 7 cut(s) 170, 230, 369, 690, 1144, 1177, 1248
BspMI ACCTGC 2 cut(s) 229, 403
BspOI GCTAGC 2 cut(s) 18, 129
BspPI GGATC 5 cut(s) 163, 176, 548, 1137, 1150
BspT107I GGYRCC 1 cut(s) 1246
BspTNI GGTCTC 2 cut(s) 430, 950
BsrDI GCAATG 3 cut(s) 164, 420, 1095
BsrGI TGTACA 2 cut(s) 961, 1338
BsrI ACTGG 4 cut(s) 698, 758, 1047, 1352
BssECI CCNNGG 3 cut(s) 371, 801, 1116
BssMI GATC 5 cut(s) 168, 206, 543, 553, 1142
BssT1I CCWWGG 2 cut(s) 801, 1116
Bst2UI CCWGG 4 cut(s) 372, 1173, 1236, 1244
Bst4CI ACNGT 6 cut(s) 483, 518, 987, 1126, 1315, 1491
Bst6I CTCTTC 1 cut(s) 882
BstAUI TGTACA 2 cut(s) 961, 1338
BstC8I GCNNGC 3 cut(s) 16, 127, 155
BstDEI CTNAG 6 cut(s) 306, 489, 826, 1002, 1272, 1434
BstDSI CCRYGG 2 cut(s) 801, 1116
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 5 cut(s) 382, 911, 964, 1061, 1255
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 171, 209, 546, 556, 1145
BstMAI GTCTC 2 cut(s) 430, 950
BstMBI GATC 5 cut(s) 168, 206, 543, 553, 1142
BstMWI GCNNNNNNNGC 6 cut(s) 99, 235, 728, 1078, 1127, 1224
BstNI CCWGG 4 cut(s) 372, 1173, 1236, 1244
BstNSI RCATGY 3 cut(s) 157, 461, 1339
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 4 cut(s) 370, 1171, 1234, 1242
BstSFI CTRYAG 1 cut(s) 813
BstV1I GCAGC 1 cut(s) 550
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 168, 206, 1142
BstXI CCANNNNNNTGG 1 cut(s) 1235
BstYI RGATCY 3 cut(s) 168, 206, 1142
BsuI GTATCC 2 cut(s) 214, 1232
BsuRI GGCC 3 cut(s) 745, 904, 1351
BtgI CCRYGG 2 cut(s) 801, 1116
BtrI CACGTC 1 cut(s) 1298
BtsCI GGATG 5 cut(s) 382, 911, 964, 1061, 1255
BtsIMutI CAGTG 2 cut(s) 834, 1054
BveI ACCTGC 2 cut(s) 229, 403
Cac8I GCNNGC 3 cut(s) 16, 127, 155
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 744
CseI GACGC 1 cut(s) 1395
CsiI ACCWGGT 1 cut(s) 1242
Csp6I GTAC 9 cut(s) 188, 450, 510, 519, 755, 962, 1166, 1247, 1339
CviAII CATG 7 cut(s) 154, 225, 458, 551, 802, 1117, 1336
CviQI GTAC 9 cut(s) 188, 450, 510, 519, 755, 962, 1166, 1247, 1339
DdeI CTNAG 6 cut(s) 306, 489, 826, 1002, 1272, 1434
DpnI GATC 5 cut(s) 170, 208, 545, 555, 1144
DpnII GATC 5 cut(s) 168, 206, 543, 553, 1142
Eam1104I CTCTTC 1 cut(s) 882
EarI CTCTTC 1 cut(s) 882
Eco130I CCWWGG 2 cut(s) 801, 1116
Eco31I GGTCTC 2 cut(s) 430, 950
Eco32I GATATC 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 947
Eco91I GGTNACC 1 cut(s) 860
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 4 cut(s) 370, 1171, 1234, 1242
EcoRV GATATC 1 cut(s) 834
EcoT14I CCWWGG 2 cut(s) 801, 1116
EcoT22I ATGCAT 1 cut(s) 155
ErhI CCWWGG 2 cut(s) 801, 1116
FaeI CATG 7 cut(s) 157, 228, 461, 554, 805, 1120, 1339
FatI CATG 7 cut(s) 153, 224, 457, 550, 801, 1116, 1335
FauI CCCGC 2 cut(s) 724, 1207
FauNDI CATATG 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 539
FokI GGATG 5 cut(s) 389, 898, 951, 1048, 1242
Fsp4HI GCNGC 1 cut(s) 539
FspBI CTAG 4 cut(s) 15, 126, 447, 1137
FspI TGCGCA 1 cut(s) 94
GlaI GCGC 1 cut(s) 94
GluI GCNGC 1 cut(s) 539
HaeIII GGCC 3 cut(s) 745, 904, 1351
HapII CCGG 1 cut(s) 1301
HgaI GACGC 1 cut(s) 1395
HhaI GCGC 1 cut(s) 95
Hin1II CATG 7 cut(s) 157, 228, 461, 554, 805, 1120, 1339
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 882
HindII GTYRAC 1 cut(s) 882
HinfI GANTC 6 cut(s) 133, 178, 324, 824, 908, 925
HpaII CCGG 1 cut(s) 1301
HphI GGTGA 4 cut(s) 214, 384, 415, 1401
Hpy166II GTNNAC 3 cut(s) 512, 882, 1482
Hpy188I TCNGA 6 cut(s) 490, 660, 916, 1005, 1275, 1385
Hpy188III TCNNGA 6 cut(s) 328, 551, 706, 947, 1039, 1440
Hpy8I GTNNAC 3 cut(s) 512, 882, 1482
HpyAV CCTTC 2 cut(s) 4, 1437
HpyCH4III ACNGT 6 cut(s) 483, 518, 987, 1126, 1315, 1491
HpyCH4IV ACGT 3 cut(s) 867, 1164, 1297
HpyF10VI GCNNNNNNNGC 6 cut(s) 99, 235, 728, 1078, 1127, 1224
HpyF3I CTNAG 6 cut(s) 306, 489, 826, 1002, 1272, 1434
HpySE526I ACGT 3 cut(s) 867, 1164, 1297
Hsp92II CATG 7 cut(s) 157, 228, 461, 554, 805, 1120, 1339
HspAI GCGC 1 cut(s) 93
KpnI GGTACC 1 cut(s) 1250
Kzo9I GATC 5 cut(s) 168, 206, 543, 553, 1142
LmnI GCTCC 4 cut(s) 118, 234, 493, 1181
Lsp1109I GCAGC 1 cut(s) 550
LweI GCATC 1 cut(s) 1081
MabI ACCWGGT 1 cut(s) 1242
MaeI CTAG 4 cut(s) 15, 126, 447, 1137
MaeII ACGT 3 cut(s) 867, 1164, 1297
MaeIII GTNAC 4 cut(s) 860, 1354, 1372, 1407
MalI GATC 5 cut(s) 170, 208, 545, 555, 1144
MboI GATC 5 cut(s) 168, 206, 543, 553, 1142
MboII GAAGA 4 cut(s) 56, 136, 899, 1410
MflI RGATCY 3 cut(s) 168, 206, 1142
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 4 cut(s) 331, 790, 1006, 1362
MlyI GAGTC 2 cut(s) 187, 818
MmeI TCCRAC 2 cut(s) 301, 992
Mph1103I ATGCAT 1 cut(s) 155
MroXI GAANNNNTTC 2 cut(s) 654, 1034
MseI TTAA 5 cut(s) 33, 69, 762, 1365, 1466
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 1 cut(s) 1301
MspR9I CCNGG 4 cut(s) 372, 1173, 1236, 1244
Mva1269I GAATGC 1 cut(s) 95
MvaI CCWGG 4 cut(s) 372, 1173, 1236, 1244
MwoI GCNNNNNNNGC 6 cut(s) 99, 235, 728, 1078, 1127, 1224
NcoI CCATGG 2 cut(s) 801, 1116
NdeI CATATG 1 cut(s) 149
NdeII GATC 5 cut(s) 168, 206, 543, 553, 1142
NheI GCTAGC 2 cut(s) 14, 125
NlaIII CATG 7 cut(s) 157, 228, 461, 554, 805, 1120, 1339
NlaIV GGNNCC 7 cut(s) 170, 230, 369, 690, 1144, 1177, 1248
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 1372, 1407
NsbI TGCGCA 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 155
NspI RCATGY 3 cut(s) 157, 461, 1339
PaeI GCATGC 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 947
PagI TCATGA 1 cut(s) 550
PaqCI CACCTGC 1 cut(s) 403
PciI ACATGT 1 cut(s) 457
PctI GAATGC 1 cut(s) 95
PdmI GAANNNNTTC 2 cut(s) 654, 1034
PfeI GAWTC 4 cut(s) 133, 324, 908, 925
PflMI CCANNNNNTGG 3 cut(s) 259, 938, 1367
PfoI TCCNGGA 1 cut(s) 1234
PkrI GCNGC 1 cut(s) 540
PleI GAGTC 2 cut(s) 186, 818
PpsI GAGTC 2 cut(s) 186, 818
PscI ACATGT 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 1365
Psp6I CCWGG 4 cut(s) 370, 1171, 1234, 1242
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 4 cut(s) 370, 1171, 1234, 1242
PspN4I GGNNCC 7 cut(s) 170, 230, 369, 690, 1144, 1177, 1248
PspPI GGNCC 1 cut(s) 744
PsuI RGATCY 3 cut(s) 168, 206, 1142
RsaI GTAC 9 cut(s) 189, 451, 511, 520, 756, 963, 1167, 1248, 1340
RsaNI GTAC 9 cut(s) 188, 450, 510, 519, 755, 962, 1166, 1247, 1339
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 5 cut(s) 33, 69, 762, 1365, 1466
SatI GCNGC 1 cut(s) 539
Sau3AI GATC 5 cut(s) 168, 206, 543, 553, 1142
Sau96I GGNCC 1 cut(s) 744
ScaI AGTACT 1 cut(s) 451
SchI GAGTC 2 cut(s) 187, 818
ScrFI CCNGG 4 cut(s) 372, 1173, 1236, 1244
SduI GDGCHC 1 cut(s) 508
SexAI ACCWGGT 1 cut(s) 1242
SfaNI GCATC 1 cut(s) 1081
SfcI CTRYAG 1 cut(s) 813
Sfr274I CTCGAG 1 cut(s) 947
SlaI CTCGAG 1 cut(s) 947
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 947, 1470
SmoI CTYRAG 2 cut(s) 947, 1470
SpeI ACTAGT 1 cut(s) 446
SphI GCATGC 1 cut(s) 157
Sse9I AATT 4 cut(s) 331, 790, 1006, 1362
SsiI CCGC 2 cut(s) 731, 1200
SspMI CTAG 4 cut(s) 15, 126, 447, 1137
StyD4I CCNGG 4 cut(s) 370, 1171, 1234, 1242
StyI CCWWGG 2 cut(s) 801, 1116
TaaI ACNGT 6 cut(s) 483, 518, 987, 1126, 1315, 1491
TaiI ACGT 3 cut(s) 870, 1167, 1300
TaqI TCGA 5 cut(s) 327, 340, 948, 1038, 1452
TasI AATT 4 cut(s) 331, 790, 1006, 1362
TatI WGTACW 3 cut(s) 449, 961, 1338
TfiI GAWTC 4 cut(s) 133, 324, 908, 925
Tru1I TTAA 5 cut(s) 33, 69, 762, 1365, 1466
Tru9I TTAA 5 cut(s) 33, 69, 762, 1365, 1466
TscAI CASTG 2 cut(s) 834, 1054
TseFI GTSAC 2 cut(s) 1372, 1407
TseI GCWGC 1 cut(s) 538
Tsp45I GTSAC 2 cut(s) 1372, 1407
TspDTI ATGAA 8 cut(s) 17, 136, 325, 366, 1010, 1043, 1244, 1389
TspGWI ACGGA 2 cut(s) 412, 1141
TspRI CASTG 2 cut(s) 834, 1054
Van91I CCANNNNNTGG 3 cut(s) 259, 938, 1367
VspI ATTAAT 1 cut(s) 1365
XapI RAATTY 1 cut(s) 331
XceI RCATGY 3 cut(s) 157, 461, 1339
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 1123
XhoI CTCGAG 1 cut(s) 947
XmnI GAANNNNTTC 2 cut(s) 654, 1034
XspI CTAG 4 cut(s) 15, 126, 447, 1137
ZrmI AGTACT 1 cut(s) 451
Zsp2I ATGCAT 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.