Rh5AG050600

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002149
Physical Location & Seq
Reverse (-)
4286334 .. 4290577
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG050600.1

Sequence Viewer

Length: 1434 bp
ATGAACCAAAAGGCTAACGTTTCTAAAGAGCTTAATGCCAAGCACAGAAAGATATTGGAAGGTCTTCTTAAATTGCCAGAGAACAGAGAATGTGCAGACTGCAAAGCCATAGCTCCACGATGGGCTAGTGTCAATCTCGGGATCTTTATATGCATGCAGTGTTCTGGGGCCCATAGAAGTCTTGGGGTACACATATCAAAGGTGAGATCTGCCACTTTGGACACATGGCTTCCAGAGCAGATTGCATTTATCCAATCGATGGGAAATGAGAAATCGAACAGTTACTGGGAAGCAGAGTTGCCTCCTAATTATGACAGAGTTGGAATCGAGAATTTCATCCGTGCAAAGTATGAATCGAAAAAATGGGTTCCTAGGGATGACAAACTAAAATCACCTCCCAGGGTGACTGCGCAGAAAACTTCAGTTTATGGAGCAAGGCCTGAAATTAGAAGCAAGCATGGCGATGTCAGAAATATTGAACAGTCTTATGAGCAGAAAGTTGCTCATCGACCTGGTAGAATAAACAGGATACCACAGCAGGTTAGTGATGATTTGAAACCGAAAAACATGGAAAAGAAATCACCAGAAGCTAGTCAAAAACCACAAGAACTTGAGCAGAAAACAGAACCAGTAGCTCCAAAACCTGAACCAGCAAGTCATAGAGCTAGTGCTACTCCAATTTCTGCACCAGTTAAAGTGGACTATGCTACTGATTTGTTCAATATGCTCTCCATGGAGGAATCAAGAGGAAGTGGCTCCGTGACATTTGCCAATAACATTACATTGGCTACTATGCATTCTGCTGAAACAAAACTGGCAGAAGAGAAAAACAATGCACCAAAATCATCTGAAAGCAAGGTGCAATCAAAATATGGTATTGAGGATTTGTTTATAGATTCTCCATCAACCACTCAAACCTTTTCAGTGAAACCTCAGACTGATGTCAAGAATGACATTATAAACTTATTTGAGAAGTCAAGTATAGTTTCTCCATTCTCGATGCATCAACAACAACAACAACAACAACAAGCAATGCTCTCCCAACAACATTCATTACTAATGGCTGCTGCGCCAAATTCTAGCGGATCCCATTCCTTTTCTTCAAGTTTACAACCTGCCTCCCAAGCTGTTCACTTGCCCACACAAAATGGGCGAAGCGTTGGCCAACAAGTTCCTGGCAACCCAACGCCACCTAATCTACAATATTATAGTCAGATTGGGAATGGTTATCATGCACGTCCAATAGGCAATGCAGTTTCATACTCGACATCAAGCATGTATACGATGAGGCCGGTAGCTCCAATCAACGGTGTCAGAAGCATCGGAGGAAGCAGAGTTTCAACACCACCGCCAAAATCTTCTGTCACTCCAACTCCTTCAGGAAGGGACTTTGATTTTTCATCGTTAACTCAAGGATTGTTTACTAAACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap

Protein Analysis

477

Amino Acids

52.64

Weight (kDa)

9.49

Isoelectric Point (pI)

57.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 17 - 123 3.9e-40 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 411
Acc36I ACCTGC 2 cut(s) 529, 1123
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 1 cut(s) 1280
AciI CCGC 2 cut(s) 1083, 1349
AclI AACGTT 1 cut(s) 18
AclWI GGATC 3 cut(s) 149, 1080, 1093
AcoI YGGCCR 1 cut(s) 1162
AcsI RAATTY 2 cut(s) 331, 1075
AcuI CTGAAG 2 cut(s) 405, 1362
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 259, 1307
AgsI TTSAA 5 cut(s) 479, 556, 721, 1104, 1341
AjiI CACGTC 1 cut(s) 1238
AjnI CCWGG 3 cut(s) 398, 511, 1174
AluBI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1127, 1298
AluI AGCT 7 cut(s) 31, 113, 590, 635, 665, 1127, 1298
AlwI GGATC 3 cut(s) 149, 1080, 1093
AlwNI CAGNNNCTG 1 cut(s) 285
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 4 cut(s) 168, 437, 1162, 1289
ApaI GGGCCC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 1064, 1067
ApoI RAATTY 2 cut(s) 331, 1075
Asp700I GAANNNNTTC 1 cut(s) 63
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 2 cut(s) 412, 1072
AspS9I GGNCC 2 cut(s) 168, 169
AsuHPI GGTGA 4 cut(s) 214, 384, 415, 573
AvaI CYCGRG 1 cut(s) 137
AvrII CCTAGG 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 1164
BamHI GGATCC 1 cut(s) 1085
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 1051, 1054
BccI CCATC 3 cut(s) 114, 253, 910
BciT130I CCWGG 3 cut(s) 400, 513, 1176
BciVI GTATCC 1 cut(s) 522
BfaI CTAG 5 cut(s) 126, 372, 591, 666, 1080
BfuAI ACCTGC 2 cut(s) 529, 1123
BfuI GTATCC 1 cut(s) 522
BglII AGATCT 1 cut(s) 206
BisI GCNGC 2 cut(s) 1065, 1068
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 2 cut(s) 1066, 1069
Bme1390I CCNGG 3 cut(s) 400, 513, 1176
BmeT110I CYCGRG 1 cut(s) 137
BmgBI CACGTC 1 cut(s) 1238
BmgT120I GGNCC 2 cut(s) 168, 169
BmiI GGNNCC 5 cut(s) 169, 170, 369, 757, 1087
BmrFI CCNGG 3 cut(s) 400, 513, 1176
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 3 cut(s) 990, 1012, 1329
BmuI ACTGGG 1 cut(s) 295
BoxI GACNNNNGTC 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 56
BpuEI CTTGAG 2 cut(s) 632, 1395
Bsa29I ATCGAT 1 cut(s) 257
BsaJI CCNNGG 4 cut(s) 371, 398, 399, 732
BsaXI ACNNNNNCTCC 2 cut(s) 1357, 1387
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 1307
Bse118I RCCGGY 1 cut(s) 1291
Bse1I ACTGG 4 cut(s) 290, 629, 689, 819
Bse3DI GCAATG 2 cut(s) 1038, 1255
BseBI CCWGG 3 cut(s) 400, 513, 1176
BseCI ATCGAT 1 cut(s) 257
BseDI CCNNGG 4 cut(s) 371, 398, 399, 732
BseGI GGATG 2 cut(s) 336, 382
BseLI CCNNNNNNNGG 2 cut(s) 259, 1307
BseMI GCAATG 2 cut(s) 1038, 1255
BseMII CTCAG 1 cut(s) 947
BseNI ACTGG 4 cut(s) 290, 629, 689, 819
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 2 cut(s) 1051, 1054
BsgI GTGCAG 2 cut(s) 114, 669
BshFI GGCC 4 cut(s) 170, 439, 1164, 1291
BshVI ATCGAT 1 cut(s) 257
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 1292
BslFI GGGAC 1 cut(s) 1400
BslI CCNNNNNNNGG 2 cut(s) 259, 1307
BsmFI GGGAC 1 cut(s) 1400
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 4 cut(s) 170, 439, 1164, 1291
BsoBI CYCGRG 1 cut(s) 137
Bsp120I GGGCCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 141, 206, 1085
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 2 cut(s) 1083, 1349
BspANI GGCC 4 cut(s) 170, 439, 1164, 1291
BspCNI CTCAG 1 cut(s) 946
BspDI ATCGAT 1 cut(s) 257
BspLI GGNNCC 5 cut(s) 169, 170, 369, 757, 1087
BspMI ACCTGC 2 cut(s) 529, 1123
BspPI GGATC 3 cut(s) 149, 1080, 1093
BsrDI GCAATG 2 cut(s) 1038, 1255
BsrFI RCCGGY 1 cut(s) 1291
BsrI ACTGG 4 cut(s) 290, 629, 689, 819
BssAI RCCGGY 1 cut(s) 1291
BssECI CCNNGG 4 cut(s) 371, 398, 399, 732
BssMI GATC 3 cut(s) 141, 206, 1085
BssNAI GTATAC 1 cut(s) 1281
BssT1I CCWWGG 2 cut(s) 371, 732
Bst1107I GTATAC 1 cut(s) 1281
Bst2UI CCWGG 3 cut(s) 400, 513, 1176
Bst4CI ACNGT 3 cut(s) 281, 483, 1310
Bst6I CTCTTC 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 155, 455
BstDEI CTNAG 1 cut(s) 933
BstDSI CCRYGG 1 cut(s) 732
BstF5I GGATG 2 cut(s) 336, 382
BstHHI GCGC 2 cut(s) 412, 1072
BstKTI GATC 3 cut(s) 144, 209, 1088
BstMBI GATC 3 cut(s) 141, 206, 1085
BstMWI GCNNNNNNNGC 3 cut(s) 235, 459, 1124
BstNI CCWGG 3 cut(s) 400, 513, 1176
BstNSI RCATGY 2 cut(s) 157, 1279
BstPAI GACNNNNGTC 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 398, 511, 1174
BstSLI GKGCMC 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 1051, 1054
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 3 cut(s) 141, 206, 1085
BstYI RGATCY 3 cut(s) 141, 206, 1085
BstZ17I GTATAC 1 cut(s) 1281
Bsu15I ATCGAT 1 cut(s) 257
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 4 cut(s) 170, 439, 1164, 1291
BsuTUI ATCGAT 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 732
BtgZI GCGATG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 1238
BtsCI GGATG 2 cut(s) 336, 382
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 164, 930
BveI ACCTGC 2 cut(s) 529, 1123
Cac8I GCNNGC 2 cut(s) 155, 455
CaiI CAGNNNCTG 1 cut(s) 285
CfoI GCGC 2 cut(s) 412, 1072
Cfr10I RCCGGY 1 cut(s) 1291
Cfr13I GGNCC 2 cut(s) 168, 169
ClaI ATCGAT 1 cut(s) 257
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 1 cut(s) 188
CviAII CATG 7 cut(s) 154, 225, 458, 568, 733, 1232, 1276
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 1 cut(s) 933
DpnI GATC 3 cut(s) 143, 208, 1087
DpnII GATC 3 cut(s) 141, 206, 1085
EaeI YGGCCR 1 cut(s) 1162
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
Eco130I CCWWGG 2 cut(s) 371, 732
Eco147I AGGCCT 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 405, 1362
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 168
EcoRII CCWGG 3 cut(s) 398, 511, 1174
EcoT14I CCWWGG 2 cut(s) 371, 732
EcoT22I ATGCAT 3 cut(s) 155, 798, 1005
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 371, 732
FaeI CATG 7 cut(s) 157, 228, 461, 571, 736, 1235, 1279
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 1400
FatI CATG 7 cut(s) 153, 224, 457, 567, 732, 1231, 1275
FblI GTMKAC 1 cut(s) 1280
Fnu4HI GCNGC 2 cut(s) 1065, 1068
FokI GGATG 2 cut(s) 323, 389
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 2 cut(s) 1065, 1068
FspBI CTAG 5 cut(s) 126, 372, 591, 666, 1080
FspI TGCGCA 1 cut(s) 411
GlaI GCGC 2 cut(s) 411, 1071
GluI GCNGC 2 cut(s) 1065, 1068
HaeIII GGCC 4 cut(s) 170, 439, 1164, 1291
HapII CCGG 1 cut(s) 1292
HhaI GCGC 2 cut(s) 412, 1072
Hin1II CATG 7 cut(s) 157, 228, 461, 571, 736, 1235, 1279
Hin6I GCGC 2 cut(s) 410, 1070
HinP1I GCGC 2 cut(s) 410, 1070
HincII GTYRAC 1 cut(s) 1407
HindII GTYRAC 1 cut(s) 1407
HinfI GANTC 4 cut(s) 324, 353, 740, 896
HpaI GTTAAC 1 cut(s) 1407
HpaII CCGG 1 cut(s) 1292
HphI GGTGA 4 cut(s) 214, 384, 415, 573
Hpy166II GTNNAC 7 cut(s) 190, 700, 1109, 1132, 1281, 1407, 1422
Hpy188I TCNGA 6 cut(s) 470, 850, 936, 1215, 1316, 1325
Hpy188III TCNNGA 7 cut(s) 139, 233, 328, 744, 946, 997, 1380
Hpy8I GTNNAC 7 cut(s) 190, 700, 1109, 1132, 1281, 1407, 1422
HpyAV CCTTC 3 cut(s) 53, 1377, 1386
HpyCH4III ACNGT 3 cut(s) 281, 483, 1310
HpyCH4IV ACGT 2 cut(s) 18, 1237
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 459, 1124
HpyF3I CTNAG 1 cut(s) 933
HpySE526I ACGT 2 cut(s) 18, 1237
Hsp92II CATG 7 cut(s) 157, 228, 461, 571, 736, 1235, 1279
HspAI GCGC 2 cut(s) 410, 1070
KspAI GTTAAC 1 cut(s) 1407
Kzo9I GATC 3 cut(s) 141, 206, 1085
LmnI GCTCC 5 cut(s) 118, 431, 640, 761, 1303
Lsp1109I GCAGC 2 cut(s) 1051, 1054
LweI GCATC 3 cut(s) 990, 1012, 1329
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 5 cut(s) 126, 372, 591, 666, 1080
MaeII ACGT 2 cut(s) 18, 1237
MaeIII GTNAC 4 cut(s) 281, 403, 760, 1363
MalI GATC 3 cut(s) 143, 208, 1087
MboI GATC 3 cut(s) 141, 206, 1085
MboII GAAGA 4 cut(s) 56, 833, 1092, 1350
MflI RGATCY 3 cut(s) 141, 206, 1085
MhlI GDGCHC 1 cut(s) 172
MlsI TGGCCA 1 cut(s) 1164
MluCI AATT 6 cut(s) 71, 307, 331, 444, 678, 1075
MluNI TGGCCA 1 cut(s) 1164
MmeI TCCRAC 2 cut(s) 301, 1394
MnlI CCTC 9 cut(s) 312, 405, 730, 740, 874, 942, 1129, 1281, 1319
Mox20I TGGCCA 1 cut(s) 1164
Mph1103I ATGCAT 3 cut(s) 155, 798, 1005
MroXI GAANNNNTTC 1 cut(s) 63
MscI TGGCCA 1 cut(s) 1164
MseI TTAA 4 cut(s) 33, 69, 693, 1406
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 1164
MspI CCGG 1 cut(s) 1292
MspR9I CCNGG 3 cut(s) 400, 513, 1176
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 3 cut(s) 400, 513, 1176
MwoI GCNNNNNNNGC 3 cut(s) 235, 459, 1124
NcoI CCATGG 1 cut(s) 732
NdeII GATC 3 cut(s) 141, 206, 1085
NlaIII CATG 7 cut(s) 157, 228, 461, 571, 736, 1235, 1279
NlaIV GGNNCC 5 cut(s) 169, 170, 369, 757, 1087
NmuCI GTSAC 3 cut(s) 403, 760, 1363
NsbI TGCGCA 1 cut(s) 411
NsiI ATGCAT 3 cut(s) 155, 798, 1005
NspI RCATGY 2 cut(s) 157, 1279
PaeI GCATGC 1 cut(s) 157
PasI CCCWGGG 1 cut(s) 399
PceI AGGCCT 1 cut(s) 439
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 324, 353, 740, 896
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 2 cut(s) 1066, 1069
PshAI GACNNNNGTC 1 cut(s) 941
PsiI TTATAA 1 cut(s) 959
Psp1406I AACGTT 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 398, 511, 1174
PspGI CCWGG 3 cut(s) 398, 511, 1174
PspN4I GGNNCC 5 cut(s) 169, 170, 369, 757, 1087
PspOMI GGGCCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 168, 169
PstNI CAGNNNCTG 1 cut(s) 285
PsuI RGATCY 3 cut(s) 141, 206, 1085
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 4 cut(s) 33, 69, 693, 1406
SatI GCNGC 2 cut(s) 1065, 1068
Sau3AI GATC 3 cut(s) 141, 206, 1085
Sau96I GGNCC 2 cut(s) 168, 169
ScrFI CCNGG 3 cut(s) 400, 513, 1176
SduI GDGCHC 1 cut(s) 172
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 3 cut(s) 990, 1012, 1329
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 2 cut(s) 611, 1410
SmoI CTYRAG 2 cut(s) 611, 1410
SphI GCATGC 1 cut(s) 157
Sse9I AATT 6 cut(s) 71, 307, 331, 444, 678, 1075
SseBI AGGCCT 1 cut(s) 439
SsiI CCGC 2 cut(s) 1083, 1349
SspI AATATT 2 cut(s) 475, 1205
SspMI CTAG 5 cut(s) 126, 372, 591, 666, 1080
StuI AGGCCT 1 cut(s) 439
StyD4I CCNGG 3 cut(s) 398, 511, 1174
StyI CCWWGG 2 cut(s) 371, 732
TaaI ACNGT 3 cut(s) 281, 483, 1310
TaiI ACGT 2 cut(s) 21, 1240
TaqI TCGA 7 cut(s) 257, 275, 327, 356, 508, 998, 1265
TasI AATT 6 cut(s) 71, 307, 331, 444, 678, 1075
TfiI GAWTC 4 cut(s) 324, 353, 740, 896
Tru1I TTAA 4 cut(s) 33, 69, 693, 1406
Tru9I TTAA 4 cut(s) 33, 69, 693, 1406
TscAI CASTG 2 cut(s) 164, 930
TseFI GTSAC 3 cut(s) 403, 760, 1363
TseI GCWGC 2 cut(s) 1064, 1067
Tsp45I GTSAC 3 cut(s) 403, 760, 1363
TspDTI ATGAA 6 cut(s) 17, 325, 366, 1041, 1248, 1389
TspGWI ACGGA 2 cut(s) 329, 748
TspRI CASTG 2 cut(s) 164, 930
Van91I CCANNNNNTGG 1 cut(s) 259
XapI RAATTY 2 cut(s) 331, 1075
XceI RCATGY 2 cut(s) 157, 1279
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 1172
XmaJI CCTAGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 1280
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 5 cut(s) 126, 372, 591, 666, 1080
Zsp2I ATGCAT 3 cut(s) 155, 798, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.